ATE408710T1 - Nachweis von legionella durch ein pcr und fret verwendendes verfahren - Google Patents
Nachweis von legionella durch ein pcr und fret verwendendes verfahrenInfo
- Publication number
- ATE408710T1 ATE408710T1 AT02731686T AT02731686T ATE408710T1 AT E408710 T1 ATE408710 T1 AT E408710T1 AT 02731686 T AT02731686 T AT 02731686T AT 02731686 T AT02731686 T AT 02731686T AT E408710 T1 ATE408710 T1 AT E408710T1
- Authority
- AT
- Austria
- Prior art keywords
- legionella
- detection
- pcr
- fret
- pneumophila
- Prior art date
Links
- 241000589248 Legionella Species 0.000 title abstract 4
- 208000007764 Legionnaires' Disease Diseases 0.000 title abstract 4
- 238000001514 detection method Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 241000589242 Legionella pneumophila Species 0.000 abstract 3
- 239000000523 sample Substances 0.000 abstract 2
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000003753 real-time PCR Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Diaphragms For Electromechanical Transducers (AREA)
- Ropes Or Cables (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28922201P | 2001-05-07 | 2001-05-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE408710T1 true ATE408710T1 (de) | 2008-10-15 |
Family
ID=23110577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02731686T ATE408710T1 (de) | 2001-05-07 | 2002-05-07 | Nachweis von legionella durch ein pcr und fret verwendendes verfahren |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US6830888B2 (de) |
| EP (1) | EP1392362B1 (de) |
| AT (1) | ATE408710T1 (de) |
| DE (1) | DE60228952D1 (de) |
| WO (1) | WO2002089844A1 (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7615780B2 (en) * | 2000-10-23 | 2009-11-10 | General Electric Company | DNA biosensor and methods for making and using the same |
| JP2005523701A (ja) * | 2002-04-26 | 2005-08-11 | ユニバーシティ・オブ・ユタ・リサーチ・ファウンデーション | 二本鎖核酸−特異的色素を使用する二次構造の融解分析による一本鎖核酸の特徴付け |
| US7282331B2 (en) * | 2003-03-18 | 2007-10-16 | Advandx, Inc. | Method for improved specificity in probe based assays |
| KR100681837B1 (ko) * | 2005-06-09 | 2007-02-12 | 건국대학교 산학협력단 | 리얼타임 pcr을 이용한 리케챠 및 스크럽 티푸스그룹(stg)균의 검출방법 |
| EP2400037A1 (de) * | 2005-10-17 | 2011-12-28 | Gen-Probe Incorporated | Zusammensetzungen und Verfahren zum Nachweisen von Legionella-pneumophila-Nukleinsäure |
| US8609829B2 (en) * | 2005-10-17 | 2013-12-17 | Gen-Probe Incorporated | Compositions and methods to detect Legionella pneumophila nucleic acid |
| EP1852512B1 (de) * | 2006-05-02 | 2011-11-02 | Universite Pierre Et Marie Curie | Methode zur Bestimmung und Auszählung von Mikroorganismen |
| US8535888B2 (en) * | 2006-12-29 | 2013-09-17 | Mayo Foundation For Medical Education And Research | Compositions and methods for detecting methicillin-resistant S. aureus |
| WO2010014672A1 (en) * | 2008-07-30 | 2010-02-04 | General Electric Company | Method for evaluating the virulence of pathogenic biphasic bacteria |
| EP3502272A1 (de) * | 2010-06-21 | 2019-06-26 | Life Technologies Corporation | Zusammensetzungen, kits und verfahren zur synthese und/oder zum nachweis von nukleinsäuren |
| US10738327B2 (en) * | 2017-08-28 | 2020-08-11 | Inscripta, Inc. | Electroporation cuvettes for automation |
| BR112021015031A2 (pt) * | 2019-01-31 | 2021-10-05 | Quest Diagnostics Investments Llc | Métodos para detectar legionella |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4851333A (en) | 1984-02-24 | 1989-07-25 | Genetic Systems Corporation | Method and composition for the detection and diagnosis of Legionella pneumophila |
| US4722891A (en) | 1984-06-20 | 1988-02-02 | Board Of Regents, The University Of Texas System | Methods and compositions for detection of Legionnaires' disease |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4996143A (en) | 1985-12-23 | 1991-02-26 | Syngene, Inc. | Fluorescent stokes shift probes for polynucleotide hybridization |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US5541308A (en) | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| US5994059A (en) | 1986-11-24 | 1999-11-30 | Gen-Probe Incorporated | Nucleic acid probes and methods for detecting Streptomyces enterococci |
| US5035996A (en) | 1989-06-01 | 1991-07-30 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
| US5683896A (en) | 1989-06-01 | 1997-11-04 | Life Technologies, Inc. | Process for controlling contamination of nucleic acid amplification reactions |
| US5491225A (en) * | 1991-12-19 | 1996-02-13 | Hoffmann-La Roche Inc. | PCR primers for detection of legionella species and methods for controlling visual intensity in hybridization assays |
| WO1993009128A1 (en) * | 1991-11-07 | 1993-05-13 | Nanotronics, Inc. | Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system |
| WO1994028174A1 (en) | 1993-05-24 | 1994-12-08 | Amoco Corporation | Nucleic acid probes for bacteria of the genus legionella |
| US6174670B1 (en) | 1996-06-04 | 2001-01-16 | University Of Utah Research Foundation | Monitoring amplification of DNA during PCR |
| WO1997046707A2 (en) | 1996-06-04 | 1997-12-11 | University Of Utah Research Foundation | System and method for monitoring for dna amplification by fluorescence |
| US5968739A (en) * | 1996-12-10 | 1999-10-19 | Abbott Laboratories | Nucleic acid primers and probes for detecting Legionella pneumophila |
| WO1998048046A2 (en) | 1997-04-22 | 1998-10-29 | Bavarian Nordic Research Institute A/S | Taqmantm-pcr for the detection of pathogenic e. coli strains |
| GB9803382D0 (en) * | 1998-02-19 | 1998-04-15 | Secr Defence | Detection system |
| JP2000184894A (ja) * | 1998-10-15 | 2000-07-04 | Japan Bioindustry Association | 一対の核酸プロ―ブおよびそのプロ―ブを用いる複合微生物系または共生微生物系における特定グル―プ若しくは特定菌の存在量を測定する方法 |
| US6162605A (en) | 1999-04-12 | 2000-12-19 | Becton Dickinson And Company | Amplification and detection of shiga-like toxin I producing organisms |
| US6387621B1 (en) * | 1999-04-27 | 2002-05-14 | University Of Utah Research Foundation | Automated analysis of real-time nucleic acid amplification |
| US6251609B1 (en) | 2000-07-27 | 2001-06-26 | Becton, Dickinson And Company | Amplification and detection of Legionella pneumophila targeting the mip gene |
-
2002
- 2002-05-07 AT AT02731686T patent/ATE408710T1/de not_active IP Right Cessation
- 2002-05-07 US US10/140,741 patent/US6830888B2/en not_active Expired - Lifetime
- 2002-05-07 DE DE60228952T patent/DE60228952D1/de not_active Expired - Lifetime
- 2002-05-07 WO PCT/US2002/014313 patent/WO2002089844A1/en not_active Ceased
- 2002-05-07 EP EP02731686A patent/EP1392362B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US6830888B2 (en) | 2004-12-14 |
| EP1392362A1 (de) | 2004-03-03 |
| DE60228952D1 (de) | 2008-10-30 |
| WO2002089844A1 (en) | 2002-11-14 |
| US20030082577A1 (en) | 2003-05-01 |
| EP1392362A4 (de) | 2006-08-30 |
| EP1392362B1 (de) | 2008-09-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties | ||
| REN | Ceased due to non-payment of the annual fee |