CA2591550C - Monitoring hybridization during pcr - Google Patents

Monitoring hybridization during pcr Download PDF

Info

Publication number
CA2591550C
CA2591550C CA002591550A CA2591550A CA2591550C CA 2591550 C CA2591550 C CA 2591550C CA 002591550 A CA002591550 A CA 002591550A CA 2591550 A CA2591550 A CA 2591550A CA 2591550 C CA2591550 C CA 2591550C
Authority
CA
Canada
Prior art keywords
amplification
fluorescence
nucleic acid
temperature
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002591550A
Other languages
English (en)
French (fr)
Other versions
CA2591550A1 (en
Inventor
Carl T. Wittwer
Kirk M. Ririe
Randy P. Rasmussen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Utah Research Foundation Inc
Original Assignee
University of Utah Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27097737&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2591550(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University of Utah Research Foundation Inc filed Critical University of Utah Research Foundation Inc
Priority to CA2658290A priority Critical patent/CA2658290C/en
Publication of CA2591550A1 publication Critical patent/CA2591550A1/en
Application granted granted Critical
Publication of CA2591550C publication Critical patent/CA2591550C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Fats And Perfumes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
CA002591550A 1996-06-04 1997-06-04 Monitoring hybridization during pcr Expired - Lifetime CA2591550C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2658290A CA2658290C (en) 1996-06-04 1997-06-04 Monitoring hybridization during pcr using fluorescent dye specific to double-stranded dna

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US65899396A 1996-06-04 1996-06-04
US08/658,993 1996-06-04
US81826797A 1997-03-17 1997-03-17
US08/818,267 1997-03-17
CA002257109A CA2257109C (en) 1996-06-04 1997-06-04 Monitoring hybridization during pcr

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA002257109A Division CA2257109C (en) 1996-06-04 1997-06-04 Monitoring hybridization during pcr

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA2658290A Division CA2658290C (en) 1996-06-04 1997-06-04 Monitoring hybridization during pcr using fluorescent dye specific to double-stranded dna

Publications (2)

Publication Number Publication Date
CA2591550A1 CA2591550A1 (en) 1997-12-11
CA2591550C true CA2591550C (en) 2009-04-28

Family

ID=27097737

Family Applications (3)

Application Number Title Priority Date Filing Date
CA002591550A Expired - Lifetime CA2591550C (en) 1996-06-04 1997-06-04 Monitoring hybridization during pcr
CA002257109A Expired - Lifetime CA2257109C (en) 1996-06-04 1997-06-04 Monitoring hybridization during pcr
CA2658290A Expired - Lifetime CA2658290C (en) 1996-06-04 1997-06-04 Monitoring hybridization during pcr using fluorescent dye specific to double-stranded dna

Family Applications After (2)

Application Number Title Priority Date Filing Date
CA002257109A Expired - Lifetime CA2257109C (en) 1996-06-04 1997-06-04 Monitoring hybridization during pcr
CA2658290A Expired - Lifetime CA2658290C (en) 1996-06-04 1997-06-04 Monitoring hybridization during pcr using fluorescent dye specific to double-stranded dna

Country Status (12)

Country Link
US (9) US6174670B1 (enExample)
EP (3) EP1033411B1 (enExample)
JP (4) JP4540754B2 (enExample)
AT (3) ATE318327T1 (enExample)
AU (1) AU726501B2 (enExample)
CA (3) CA2591550C (enExample)
DE (3) DE69733282T2 (enExample)
DK (2) DK1179600T3 (enExample)
ES (2) ES2326050T5 (enExample)
NZ (2) NZ333136A (enExample)
PT (2) PT1179600E (enExample)
WO (1) WO1997046714A1 (enExample)

Families Citing this family (800)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070269799A9 (en) * 1994-06-22 2007-11-22 Zhang David Y Nucleic acid amplification methods
ATE318327T1 (de) * 1996-06-04 2006-03-15 Univ Utah Res Found Fluoreszenz-donor-akzeptor paar
WO1998036096A1 (en) * 1997-02-14 1998-08-20 E.I. Du Pont De Nemours And Company Detection of double-stranded dna in a homogeneous solution
US20030165888A1 (en) * 2001-07-18 2003-09-04 Brown Bob D. Oligonucleotide probes and primers comprising universal bases for diagnostic purposes
US6518017B1 (en) * 1997-10-02 2003-02-11 Oasis Biosciences Incorporated Combinatorial antisense library
US6355421B1 (en) 1997-10-27 2002-03-12 Boston Probes, Inc. Methods, kits and compositions pertaining to PNA molecular beacons
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
JP4502502B2 (ja) * 1997-10-28 2010-07-14 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア フローサイトメトリーを用いるｄｎａ多型の同定方法
AU741141B2 (en) * 1997-11-04 2001-11-22 Roche Diagnostics Gmbh Specific and sensitive method for detecting nucleic acids
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
WO1999040219A1 (en) * 1998-02-05 1999-08-12 Bavarian Nordic Research Institute A/S Quantification by inhibition of amplification
GB9803382D0 (en) * 1998-02-19 1998-04-15 Secr Defence Detection system
US6270973B1 (en) * 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
DE19811729C2 (de) * 1998-03-18 2000-05-18 November Ag Molekulare Medizin Verfahren und Vorrichtung zum Nachweis einer Nukleotidsequenz
DE19811731A1 (de) * 1998-03-18 1999-09-23 November Ag Molekulare Medizin Verfahren zum Nachweis einer Nukleotidsequenz
US6361942B1 (en) 1998-03-24 2002-03-26 Boston Probes, Inc. Method, kits and compositions pertaining to detection complexes
US6132968A (en) * 1998-05-13 2000-10-17 University Of Alberta Methods for quantitating low level modifications of nucleotide sequences
GB9811483D0 (en) * 1998-05-29 1998-07-29 Photonic Research Systems Limi Luminescence assay using cyclical excitation wavelength sequence
US7799521B2 (en) * 1998-06-24 2010-09-21 Chen & Chen, Llc Thermal cycling
US6780617B2 (en) 2000-12-29 2004-08-24 Chen & Chen, Llc Sample processing device and method
GB9815799D0 (en) * 1998-07-21 1998-09-16 Pharmagene Lab Limited Quantitative analysis of gene expression
GB9819417D0 (en) 1998-09-07 1998-10-28 Secr Defence Reaction method
GB9819418D0 (en) * 1998-09-07 1998-10-28 Secr Defence Amplification method
DE19840897A1 (de) * 1998-09-08 2000-03-09 Dirk Happich Faktor V-Leiden-Test
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
GB9821989D0 (en) * 1998-10-08 1998-12-02 Hybaid Ltd Detection of nucleic acid polymorphism
DE19850593A1 (de) * 1998-11-03 2000-05-04 Biochip Technologies Gmbh Verfahren zum Nachweis von Hybridisierungsereignissen bei DNA
US6216049B1 (en) * 1998-11-20 2001-04-10 Becton, Dickinson And Company Computerized method and apparatus for analyzing nucleic acid assay readings
EP1013775A1 (en) * 1998-12-21 2000-06-28 LUTZ, Hans Quantitative polymerase chain reaction using a fluorogenic real-time detection system
GB9901072D0 (en) * 1999-01-19 1999-03-10 Imp Cancer Res Tech Methods for detecting changes to a macromolecular component of a cell
US6306578B1 (en) * 1999-03-19 2001-10-23 Genencor International, Inc. Multi-through hole testing plate for high throughput screening
US6303305B1 (en) 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
KR20020013519A (ko) * 1999-04-08 2002-02-20 추후제출 보편적 및/또는 축퇴성 염기를 포함하는 안티센스올리고뉴클레오티드
GB9908437D0 (en) * 1999-04-13 1999-06-09 Minton Treharne & Davies Limit Methods of marking materials
US6699661B1 (en) * 1999-04-20 2004-03-02 Kankyo Engineering Co., Ltd. Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
US6387621B1 (en) * 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
JP2003510017A (ja) * 1999-06-22 2003-03-18 インビトロジェン コーポレイション 核酸の検出および識別のために改良されたプライマーおよび方法
AU7627100A (en) * 1999-07-27 2001-02-13 University Of Utah Research Foundation Homogeneous fluorescence method for assaying structural modifications of biomolecules
US6197520B1 (en) * 1999-08-13 2001-03-06 University Of Utah Research Foundation Solution-based color compensation adjusted for temperature and electronic gains
US7192698B1 (en) * 1999-08-17 2007-03-20 Purdue Research Foundation EphA2 as a diagnostic target for metastatic cancer
US6927203B1 (en) * 1999-08-17 2005-08-09 Purdue Research Foundation Treatment of metastatic disease
US20040170966A1 (en) * 1999-11-29 2004-09-02 Furfine Eric Steven Continuous time-resolved resonance energy-transfer assay for polynucleic acid polymerases
GB9928232D0 (en) * 1999-12-01 2000-01-26 Skelton Stephen Detection system
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
US7700279B2 (en) * 1999-12-24 2010-04-20 Quest Diagnostics Investments Incorporated Assay for bcr/abl gene rearrangement
US7250252B2 (en) * 1999-12-30 2007-07-31 David Aaron Katz Amplification based polymorphism detection
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
GB0005281D0 (en) * 2000-03-07 2000-04-26 Secr Defence Analytical method
US6691041B2 (en) * 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids
DE10045521A1 (de) * 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen
DE10018465A1 (de) * 2000-04-14 2001-10-18 Aventis Res & Tech Gmbh & Co Testsystem zum Nachweis einer Spleißreaktion, sowie dessen Verwendung
US6783934B1 (en) 2000-05-01 2004-08-31 Cepheid, Inc. Methods for quantitative analysis of nucleic acid amplification reaction
EP1158449A3 (en) * 2000-05-19 2004-12-15 Becton Dickinson and Company Computerized method and apparatus for analyzing readings of nucleic acid assays
GB0016516D0 (en) * 2000-07-06 2000-08-23 Owen Mumford Ltd Improvements relating to needle protection devices
NZ523507A (en) 2000-07-07 2005-01-28 Aventis Pharma Inc High throughput, possibly automated, process for transposition-mediated sequencing of target DNA fragments in which the amount of non-target DNA sequence is minimised
CA2417986C (en) 2000-08-11 2013-11-26 University Of Utah Research Foundation Single-labeled oligonucleotide probes
EP1360324A2 (en) 2000-08-31 2003-11-12 Mayo Foundation For Medical Education And Research Detection of varicella-zoster virus
US7101976B1 (en) 2000-09-12 2006-09-05 Purdue Research Foundation EphA2 monoclonal antibodies and methods of making and using same
US6558929B2 (en) * 2000-09-15 2003-05-06 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forshung E.V. PCR reaction mixture for fluorescence-based gene expression and gene mutation analyses
US6346386B1 (en) * 2000-09-29 2002-02-12 Arup Institue Method of solution-based scanning for alterations in a DNA segment using a double-stranded DNA binding dye and fluorescence melting profiles
WO2002034942A2 (en) * 2000-10-23 2002-05-02 Cancer Research Technology Limited Nucleic acid amplification-based methods for the determination of a methylation profile and reagents therefor
GB0025913D0 (en) * 2000-10-23 2000-12-06 Guldberg Per Materials and methods relating to nucleic acid amplification and profiling
US8900811B2 (en) * 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
EP1337851B1 (de) * 2000-11-16 2005-07-20 Roche Diagnostics GmbH Farbstoffpaare für fluoreszenz-resonanz-energie-transfer (fret) messungen
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
WO2002061141A1 (en) * 2001-01-31 2002-08-08 Mayo Foundation For Medical Education And Research Detection of bordetella
WO2002061390A2 (en) 2001-01-31 2002-08-08 Mayo Foundation For Medical Education And Research Detection of herpes simplex virus
US7691571B2 (en) * 2001-01-31 2010-04-06 Mayo Foundation For Medical Education And Research Detection of bordetella
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US7559879B2 (en) * 2001-04-16 2009-07-14 Brunswick Corporation Stride adjustment mechanism
WO2002089844A1 (en) 2001-05-07 2002-11-14 Mayo Foundation For Medical Education And Research Legionella detection with fluorescent resonance energy transfer
US6979530B2 (en) * 2001-05-21 2005-12-27 Applera Corporation Peptide conjugates and fluorescence detection methods for intracellular caspase assay
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
WO2003002959A1 (en) * 2001-06-15 2003-01-09 Mj Research, Inc. Controller for a fluorometer
MXPA03012075A (es) * 2001-06-22 2005-07-01 Marshfield Clinic Metodos y oligonucleotidos para la deteccion de salmonela sp., e. coli o157:h7 y listeria monocitogenes.
DE10132211A1 (de) * 2001-06-27 2003-01-16 Epigenomics Ag Nachweis spezifischer Dinukleotide in DNA-Proben durch Fluoreszenzresonanzenergietransfer (FRET)
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
DE10132785A1 (de) * 2001-07-06 2003-01-16 Clondiag Chip Tech Gmbh Verfahren zum Nachweis von in einer Polymerase-Kettenreaktion amplifizierten Nukleinsäuremolekülen
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
ATE522776T1 (de) * 2001-07-16 2011-09-15 Idaho Technology Inc Temperaturwechselsystem und verwendungsverfahren
US7226732B2 (en) * 2001-07-16 2007-06-05 Cepheid Methods, apparatus, and computer programs for verifying the integrity of a probe
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
US6979541B1 (en) 2001-07-26 2005-12-27 University Of Utah Research Foundation Methods for identifying chromosomal aneuploidy
US7188030B2 (en) * 2001-08-21 2007-03-06 Applera Corporation Automatic threshold setting for quantitative polymerase chain reaction
US20050175987A1 (en) * 2001-08-23 2005-08-11 Kathrin Jansen Fluorescent multiplex hpv pcr assays using multiple fluorophores
ATE335850T1 (de) * 2001-08-31 2006-09-15 Univ Utah Res Found Echtzeit-quantifizierung mit internen standards
CN1262351C (zh) * 2001-09-11 2006-07-05 伊库姆有限公司 试样容器
US20030082563A1 (en) * 2001-10-15 2003-05-01 Bell Constance A. Detection of bacillus anthracis
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
EP1308524B1 (en) * 2001-11-02 2006-05-31 Roche Diagnostics GmbH Detection of variola virus
DE60235491D1 (de) * 2001-11-28 2010-04-08 Bio Rad Laboratories Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
US7424013B1 (en) * 2001-12-20 2008-09-09 Applied Micro Circuits Corporation System and method for granting arbitrated bids in the switching of information
US8418129B1 (en) 2001-12-14 2013-04-09 Qualcomm Incorporated Method for automatically generating code to define a system of hardware elements
US7352694B1 (en) * 2001-12-14 2008-04-01 Applied Micro Circuits Corporation System and method for tolerating data link faults in a packet communications switch fabric
JP2005516300A (ja) 2002-01-25 2005-06-02 アプレラ コーポレイション 製品およびサービスに対する注文を発注し、受理し、および充足する方法
US7228237B2 (en) * 2002-02-07 2007-06-05 Applera Corporation Automatic threshold setting and baseline determination for real-time PCR
US7373253B2 (en) 2002-02-12 2008-05-13 Idaho Technology Multi-test analysis of real-time nucleic acid amplification
US6593093B1 (en) 2002-02-20 2003-07-15 Mayo Foundation For Medical Education And Research Detection of group a Streptococcus
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
US20050065330A1 (en) * 2002-04-24 2005-03-24 The Cleveland Clinic Foundation Identification of Histoplasma capsulatum using a PCR assay
US7297484B2 (en) * 2002-04-26 2007-11-20 Idaho Technology Characterization of single stranded nucleic acids by melting analysis of secondary structure using double strand-specific nucleic acid dye
AUPS205802A0 (en) * 2002-05-01 2002-06-06 Bio-Molecular Holdings Pty Limited Improved cycling device and method
US7785776B2 (en) * 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
US20030215814A1 (en) * 2002-05-17 2003-11-20 Cockerill Franklin R. Detection of Shiga toxin- or Shiga-like toxin-producing organisms
AUPS267802A0 (en) 2002-05-30 2002-06-20 Bio-Molecular Holdings Pty Limited Improved dna amplification apparatus and method
EP1509624A2 (en) * 2002-05-31 2005-03-02 Secretary, Department Of Atomic Energy Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands
AU2003253651C1 (en) 2002-06-14 2010-06-03 Gen-Probe Incorporated Compositions and methods for detecting hepatitis B virus
US6896727B2 (en) * 2002-06-28 2005-05-24 Seh America, Inc. Method of determining nitrogen concentration within a wafer
US7083974B2 (en) * 2002-07-12 2006-08-01 Applera Corporation Rotatable sample disk and method of loading a sample disk
US20040219557A1 (en) * 2002-08-01 2004-11-04 Dobrowolski Steven F. Real time PCR assays to detect mutations in the biotinidase gene for newborn screening
US8277753B2 (en) * 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
US9394332B2 (en) * 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
PT2143803T (pt) * 2002-09-03 2016-07-14 Quanta Biosciences Inc Composições e métodos melhorados para a síntese de adnc
JP2005538734A (ja) * 2002-09-17 2005-12-22 フアルマシア・コーポレーシヨン 自動液体処理中のｒｎａ及び逆転写酵素の保存
ATE386111T1 (de) * 2002-09-18 2008-03-15 Univ Pennsylvania Zusammensetzungen, verfahren und kits zum nachweis eines antigens auf einer zelle und in einem biologischen gemisch
US7074598B2 (en) * 2002-09-25 2006-07-11 Mayo Foundation For Medical Education And Research Detection of vancomycin-resistant enterococcus spp.
US7365176B2 (en) 2002-09-26 2008-04-29 Mayo Foundation For Medical Education And Research Detection of Epstein-Barr virus
US7074599B2 (en) 2002-09-27 2006-07-11 Mayo Foundation For Medical Education And Research Detection of mecA-containing Staphylococcus spp.
ES2315397T3 (es) * 2002-09-30 2009-04-01 F. Hoffmann-La Roche Ag Oligonucleotidos para obtener el genotipo del gen de timidilato sintasa.
US9850381B2 (en) * 2002-10-23 2017-12-26 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
US7582429B2 (en) 2002-10-23 2009-09-01 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
CN105349657A (zh) 2002-12-06 2016-02-24 霍夫曼-拉罗奇有限公司 病原生物的多种分析检测
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
US20060094108A1 (en) * 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
US8275554B2 (en) * 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
CA2510166A1 (en) 2002-12-20 2004-09-30 Caliper Life Sciences, Inc. Single molecule amplification and detection of dna
DE60319146T2 (de) * 2002-12-20 2009-07-09 Stratagene, La Jolla Zusammensetzungen und verfahren für den nachweis von polynukleotiden
US20050042639A1 (en) * 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
US7560231B2 (en) * 2002-12-20 2009-07-14 Roche Molecular Systems, Inc. Mannitol and glucitol derivatives
CA2521999A1 (en) 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
DE602004009038T3 (de) * 2003-01-29 2015-06-25 Roche Diagnostics Gmbh Verbessertes Verfahren zur Behandlung durch Bisulfit
EP1597602A4 (en) * 2003-02-05 2009-07-22 Ge Healthcare Bio Sciences SOLID PHASE SEQUENCING
EP2574400B1 (en) * 2003-02-05 2016-09-28 Iquum, Inc. Sample processing
JP4608483B2 (ja) * 2003-02-18 2011-01-12 アプライド バイオシステムズ， エルエルシー ポリヌクレオチドの複合的分析のための組成物および方法
US7282331B2 (en) * 2003-03-18 2007-10-16 Advandx, Inc. Method for improved specificity in probe based assays
EP2390352A1 (en) 2003-03-18 2011-11-30 Quantum Genetics Ireland Limited Systems and methods for improving protein and milk production of dairy herds
US20040191776A1 (en) * 2003-03-21 2004-09-30 Pei-Jer Chen Method for genotyping and quantifying Hepatitis B Virus
GB2399776A (en) * 2003-03-24 2004-09-29 Pa Knowledge Ltd Cyclical heating and cooling device and associated methods
ES2524048T3 (es) 2003-03-31 2014-12-03 F. Hoffmann-La Roche Ag Composiciones y métodos para la detección de ciertos flavivirus, incluidos los miembros del serogrupo del virus de la encefalitis japonesa
EP2284278A1 (en) 2003-04-04 2011-02-16 Roche Diagnostics GmbH Improved system for multi color real time PCR
CA2463719A1 (en) * 2003-04-05 2004-10-05 F. Hoffmann-La Roche Ag Nucleotide analogs with six membered rings
WO2004094986A2 (en) * 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
WO2005030027A2 (en) * 2003-04-29 2005-04-07 The Cleveland Clinic Foundation Salmonella detection identification
US7148043B2 (en) * 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
WO2004105947A2 (en) * 2003-05-23 2004-12-09 Bio-Rad Laboratories, Inc. Localized temperature control for spatial arrays of reaction media
US20070059701A1 (en) * 2003-06-30 2007-03-15 Astrazeneca Ab Fluorescence assays for nucleic acid polymerase activity
EP1493822A1 (de) * 2003-07-02 2005-01-05 Labor Becker, Olgemoeller & Kollegen GbR Detektionsverfahren für Nucleinsäuren mit interner Amplifikationskontrolle
GB0317343D0 (en) * 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
EP1502958A1 (en) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH New detection format for hot start real time polymerase chain reaction
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
US7788039B2 (en) 2003-09-25 2010-08-31 Roche Molecular Systems, Inc. Quantitation of nucleic acids using growth curves
WO2005043167A2 (en) * 2003-11-04 2005-05-12 Roche Diagnostic Gmbh Method for distinguishing aml subtypes with differents gene dosages
WO2005045434A2 (en) * 2003-11-04 2005-05-19 Roche Diagnostics Gmbh Method for distinguishing aml subtypes with recurring genetic aberrations
WO2005045435A2 (en) * 2003-11-04 2005-05-19 Roche Diagnostics Gmbh METHOD FOR DISTINGUISHING T(11q23)/MLL-POSITIVE LEUKEMIAS FROM T(11q23)MLL NEGATIVE LEUKEMIAS
EP1530046A1 (en) * 2003-11-04 2005-05-11 Ludwig-Maximilians-Universität München Method for distinguishing AML subtypes with aberrant and prognostically intermediate karyotypes
WO2005043164A2 (en) * 2003-11-04 2005-05-12 Roche Diagnostics Gmbh Method for distinguishing cbf-positive aml subtypes from cbf-negative aml subtypes
EP1682901A2 (en) * 2003-11-04 2006-07-26 Roche Diagnostics GmbH Method for distinguishing leukemia subtypes
WO2005045437A2 (en) * 2003-11-04 2005-05-19 Roche Diagnostics Gmbh Method for distinguishing immunologically defined all subtypes
EP1682903A2 (en) * 2003-11-04 2006-07-26 Roche Diagnostics GmbH Method for distinguishing aml-specific flt3 length mutations from tkd mutations
EP1533618A1 (en) * 2003-11-04 2005-05-25 Ludwig-Maximilians-Universität München Method for distinguishing prognostically definable AML
US20070212687A1 (en) * 2003-11-04 2007-09-13 Martin Dugas Method For Distinguishing Mll-Ptd-Positive Aml From Other Aml Subtypes
DE502004010402D1 (enExample) * 2003-11-06 2009-12-31 Juerg Dual
US7427475B2 (en) * 2003-11-18 2008-09-23 Mayo Foundation For Medical Education And Research Detection of group B streptococcus
US7667024B2 (en) * 2003-11-19 2010-02-23 Allelogic Biosciences Corp. Oligonucleotides labeled with a plurality of fluorophores
US7582452B2 (en) * 2003-11-24 2009-09-01 Life Genetics Lab, Llc Assay for species sources
CA2494571C (en) * 2003-12-02 2010-02-09 F.Hoffmann-La Roche Ag Oligonucleotides containing molecular rods
WO2005054502A1 (en) 2003-12-02 2005-06-16 Roche Diagnostics Gmbh Improved method for bisulfite treatment
US20050130213A1 (en) * 2003-12-10 2005-06-16 Tom Morrison Selective ligation and amplification assay
US8697433B2 (en) * 2003-12-10 2014-04-15 Samsung Electronics Co., Ltd. Polymerase chain reaction (PCR) module and multiple PCR system using the same
ATE485401T1 (de) 2004-02-10 2010-11-15 Hoffmann La Roche Neue primer und sonden zum nachweis von parvovirus b19
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
JP2007525217A (ja) 2004-02-19 2007-09-06 ザ ガバナーズ オブ ザ ユニバーシティー オブ アルバータ レプチンプロモーター多型及びその使用
US20050250134A1 (en) * 2004-03-02 2005-11-10 Dawei Sheng Fluorescent energy transfer labeled nucleic acid substrates and methods of use thereof
AU2005220746A1 (en) 2004-03-05 2005-09-22 Medical College Of Ohio Methods and compositions for assessing nucleic acids and alleles
WO2005089945A1 (en) 2004-03-12 2005-09-29 Biotrove, Inc. Nanoliter array loading
EP1740711A2 (en) 2004-04-01 2007-01-10 Bio-Rad Laboratories, Inc. Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity
EP1745157A4 (en) 2004-04-12 2008-06-11 Ohio Med College METHOD AND COMPOSITIONS FOR TESTING ANALYTES
EP1745120A4 (en) 2004-04-16 2010-04-14 Spartan Bioscience Inc SYSTEM FOR RAPID AMPLIFICATION AND DETECTION OF NUCLEIC ACIDS
US9657347B2 (en) 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
US7387887B2 (en) 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US20050250112A1 (en) * 2004-05-07 2005-11-10 Padmabandu Gothami A Nucleic acids, compositions, methods, and kits for detecting mycoplasma and acholeplasma species
JP5007440B2 (ja) * 2004-05-13 2012-08-22 ナノバイオシン， インコーポレイテッド ナノ−ｐｃｒ：核酸増幅及び検出のための方法及び装置
DE102004025538A1 (de) * 2004-05-25 2005-12-22 Advalytix Ag Temperierverfahren und -vorrichtung für die Temperaturbehandlung kleiner Flüssigkeitsmengen
EP2471924A1 (en) * 2004-05-28 2012-07-04 Asuragen, INC. Methods and compositions involving microRNA
EP1605060A1 (en) * 2004-06-10 2005-12-14 Biodynamics S.R.L. Methods and reaction mixture reagent for increasing the specificity and fidelity of polymerase reactions
EP1610170A1 (en) * 2004-06-25 2005-12-28 Sony Deutschland GmbH A method of applying a particle film to create a surface having light-diffusive and/or reduced glare properties
JP4782784B2 (ja) * 2004-06-25 2011-09-28 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 病原性大腸菌の検出および識別のためのｄｎａ配列
GB0416944D0 (en) 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
TWI349704B (en) * 2004-08-10 2011-10-01 Food And Drug Administration Dept Of Health A method for rapidly detecting quinolone-resistant salmonella spp. and the probes and primers utilized therein
WO2006020933A2 (en) * 2004-08-13 2006-02-23 Stratagene California Dual labeled fluorescent probes
EP1632578A1 (en) 2004-09-03 2006-03-08 Roche Diagnostics GmbH DNA decontamination method
EP2093297A3 (en) 2004-09-07 2009-11-18 Telethon Institute for Child Health Research Agents for treatment or prevention of an allergic disorder
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
US20060051796A1 (en) * 2004-09-09 2006-03-09 Inga Boell Real time PCR with the addition of pyrophosphatase
KR20070085275A (ko) * 2004-09-20 2007-08-27 유니버시티 오브 피츠버그 오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 멀티플 모드에 의한 멀티플렉스 반응 억제 방법
DE602005025782D1 (de) 2004-09-21 2011-02-17 Life Technologies Corp Zweifarbige echtzeit/endpunkt-quantifizierung von mikro-rnas (mirnas)
US20060099621A1 (en) * 2004-10-07 2006-05-11 Chronix Biomedical Detection of nucleic acids to assess risk for Creutzfeldt-Jakob disease
US20060078894A1 (en) * 2004-10-12 2006-04-13 Winkler Matthew M Methods and compositions for analyzing nucleic acids
WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification
US20060194222A1 (en) * 2004-10-20 2006-08-31 Stratagene California Triplex probe compositions and methods for polynucleotide detection
WO2006045392A2 (en) 2004-10-21 2006-05-04 GSF-Forschungszentrum für Umwelt und Gesundheit GmbH Kaspp (lrrke) gene, its production and use for the detection and treatment of neurodegenerative disorders
AU2005299590B2 (en) * 2004-10-27 2010-09-16 Cepheid Closed-system multi-stage nucleic acid amplification reactions
WO2006048262A2 (en) * 2004-11-04 2006-05-11 Roche Diagnostics Gmbh Classification of acute myeloid leukemia
WO2008073922A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Functions and targets of let-7 micro rnas
EP2302053A1 (en) 2004-11-12 2011-03-30 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
US20060188902A1 (en) * 2005-01-03 2006-08-24 The Gov. of the USA as represented by the Secretary of the Dept. of Health and Human Primer for nucleic acid detection
WO2006074233A2 (en) * 2005-01-06 2006-07-13 Applera Corporation Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US7315376B2 (en) * 2005-01-07 2008-01-01 Advanced Molecular Systems, Llc Fluorescence detection system
DE602005000877T2 (de) 2005-01-18 2007-12-20 Roche Diagnostics Gmbh Fluoreszenzabbildung mittels Telezentrizität
EP1681555B1 (en) 2005-01-18 2007-03-21 Roche Diagnostics GmbH Imaging of fluorescence signals using telecentric excitation and imaging optics
US8669052B2 (en) * 2008-06-10 2014-03-11 Rapid Pathogen Screening, Inc. Lateral flow nucleic acid detector
US8470608B2 (en) * 2008-05-20 2013-06-25 Rapid Pathogen Screening, Inc Combined visual/fluorescence analyte detection test
US9464310B2 (en) * 2005-02-10 2016-10-11 Medical Diagnostic Laboratories, Llc Integrated method for collection and maintenance of detectability of a plurality of microbiological agents in a single clinical sample and for handling a plurality of samples for reporting a sum of diagnostic results for each sample
US20060178838A1 (en) * 2005-02-10 2006-08-10 Adelson Martin E Method of receiving and handling a plurality of clinical samples for reporting a sum of diagnostic results for each sample
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US20060246423A1 (en) * 2005-02-10 2006-11-02 Adelson Martin E Method and kit for the collection and maintenance of the detectability of a plurality of microbiological species in a single gynecological sample
US20060188891A1 (en) * 2005-02-23 2006-08-24 Bickmore William D Jr Methods and apparatus for controlling DNA amplification
JP4398886B2 (ja) * 2005-03-07 2010-01-13 ソニー株式会社 通信端末装置、通信システム、通信方法、およびプログラム
US7932081B2 (en) 2005-03-10 2011-04-26 Gen-Probe Incorporated Signal measuring system for conducting real-time amplification assays
EP2348320B1 (en) 2005-03-10 2024-05-01 Gen-Probe Incorporated Methods and systems for detecting multiple fluorescent emission signals
US7759469B2 (en) 2005-03-10 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
EP1700922B1 (en) 2005-03-10 2016-08-24 Roche Diagnostics GmbH 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them
US7680604B2 (en) 2005-03-11 2010-03-16 Roche Molecular Systems, Inc. PCR elbow determination by rotational transform after zero slope alignment
US7776567B2 (en) * 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7456281B2 (en) * 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
EP1877585A2 (en) * 2005-04-28 2008-01-16 Merck & Co., Inc. Real-time hpv pcr assays
AU2006242201A1 (en) * 2005-05-02 2006-11-09 Stratagene California Oligonucleotide probe/primer compositions and methods for polynucleotide detection
US7737281B2 (en) * 2005-05-24 2010-06-15 Enzo Life Sciences, Inc. C/O Enzo Biochem, Inc. Purine based fluorescent dyes
US8362250B2 (en) 2005-05-24 2013-01-29 Enzo Biochem, Inc. Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest
US8357801B2 (en) 2005-05-24 2013-01-22 Enzo Life Sciences, Inc. Labeling of target molecules, identification of organelles and other applications, novel compositions, methods and kits
US7569695B2 (en) * 2005-05-24 2009-08-04 Enzo Life Sciences, Inc. Dyes for the detection or quantification of desirable target molecules
AU2006341607B2 (en) * 2005-05-31 2011-03-17 Applied Biosystems, Llc. Multiplexed amplification of short nucleic acids
US20060281099A1 (en) * 2005-06-14 2006-12-14 Bio-Rad Laboratories, Inc. Q-melt for polymorphism detection
EP3492602A1 (en) 2005-06-15 2019-06-05 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US20060286590A1 (en) * 2005-06-20 2006-12-21 Applera Corporation. Cis-regulatory modules
US20060292578A1 (en) * 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
KR101515821B1 (ko) * 2005-09-01 2015-04-30 오스다이어그나스틱스 피티와이 엘티디. 핵산의 증폭, 정량분석 및 동정 방법
US7569367B2 (en) 2005-09-07 2009-08-04 Roche Diagnostics Operations, Inc. Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US7991558B2 (en) 2005-09-29 2011-08-02 Roche Molecular Systems, Inc. Systems and methods for determining real-time PCR cycle thresholds using cluster analysis
US7991561B2 (en) 2005-09-29 2011-08-02 Roche Molecular Systems, Inc. Ct determination by cluster analysis with variable cluster endpoint
WO2007041201A2 (en) 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
CA2624896C (en) 2005-10-07 2017-11-07 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
WO2007047606A2 (en) * 2005-10-17 2007-04-26 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Electrokinetic thermal cycler and reactor
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
WO2007120208A2 (en) * 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
DK1954834T3 (da) 2005-11-29 2012-11-26 Cambridge Entpr Ltd Brystcancermarkører
EP1798291B1 (en) * 2005-12-13 2011-05-18 Institut Curie Methods and compositions for assaying mutations and/or large scale alterations in nucleic acids and their uses in diagnosis of genetic diseases and cancers
EP1963352B1 (en) * 2005-12-14 2010-09-08 Roche Diagnostics GmbH New method for bisulfite treatment
EP1798542A1 (en) * 2005-12-19 2007-06-20 Roche Diagnostics GmbH Analytical method and instrument
EP1798650A1 (en) * 2005-12-19 2007-06-20 Roche Diagnostics GmbH Analytical method and instrument
US7844403B2 (en) 2005-12-20 2010-11-30 Roche Molecular Systems, Inc. Temperature step correction with double sigmoid Levenberg-Marquardt and robust linear regression
US7668663B2 (en) 2005-12-20 2010-02-23 Roche Molecular Systems, Inc. Levenberg-Marquardt outlier spike removal method
US7991562B2 (en) 2005-12-20 2011-08-02 Roche Molecular Systems, Inc. PCR elbow determination using curvature analysis of a double sigmoid
US7680868B2 (en) 2005-12-20 2010-03-16 Roche Molecular Systems, Inc. PCR elbow determination by use of a double sigmoid function curve fit with the Levenburg-Marquardt algorithm and normalization
US7981606B2 (en) * 2005-12-21 2011-07-19 Roche Molecular Systems, Inc. Control for nucleic acid testing
WO2007084568A2 (en) 2006-01-17 2007-07-26 Health Research, Inc. Heteroduplex tracking assay
US20090305238A1 (en) * 2006-01-23 2009-12-10 Applera Corporation Microarray Microcard
US7785786B2 (en) 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
WO2007092538A2 (en) * 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
GB0603190D0 (en) * 2006-02-16 2006-03-29 Enigma Diagnostics Ltd Detection system
CA2638854C (en) 2006-02-27 2012-05-15 F. Hoffmann-La Roche Ag Pcr hot start by magnesium sequestration
MX2008011185A (es) * 2006-03-01 2008-09-10 Perlegen Sciences Inc Marcadores para adiccion.
US20110143344A1 (en) * 2006-03-01 2011-06-16 The Washington University Genetic polymorphisms and substance dependence
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
US8778637B2 (en) 2006-03-28 2014-07-15 Canon U.S. Life Sciences, Inc. Method and apparatus for applying continuous flow and uniform temperature to generate thermal melting curves in a microfluidic device
WO2007115059A2 (en) 2006-03-29 2007-10-11 Merial Limited Vaccine against streptococci
US8293473B2 (en) 2006-04-04 2012-10-23 Labonnet Ltd. Assessment of reaction kinetics compatibility between polymerase chain reactions
US20070238108A1 (en) * 2006-04-07 2007-10-11 Agilent Technologies, Inc. Validation of comparative genomic hybridization
US20070238093A1 (en) * 2006-04-11 2007-10-11 Espy Mark J Detection of influenza A virus
US20070238095A1 (en) * 2006-04-11 2007-10-11 Mayo Foundation For Medical Education And Research , A Minnesota Corporation Detection of Influenza A Virus
US9862984B2 (en) 2006-04-21 2018-01-09 Nanobiosym, Inc. Single-molecule platform for drug discovery: methods and apparatuses for drug discovery, including discovery of anticancer and antiviral agents
CA2650820C (en) 2006-04-28 2014-09-16 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates to improve nucleic acid detection
US8900828B2 (en) 2006-05-01 2014-12-02 Cepheid Methods and apparatus for sequential amplification reactions
US8232091B2 (en) * 2006-05-17 2012-07-31 California Institute Of Technology Thermal cycling system
EP2027251A4 (en) * 2006-05-17 2010-05-05 California Inst Of Techn Thermal cycling system
EP2029775B1 (en) * 2006-06-05 2014-10-15 California Institute Of Technology Real time micro arrays
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8637436B2 (en) 2006-08-24 2014-01-28 California Institute Of Technology Integrated semiconductor bioarray
US8119352B2 (en) 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
US7629124B2 (en) * 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008021010A2 (en) * 2006-08-07 2008-02-21 Stratagene California Methods for real-time quantitative pcr
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US8080645B2 (en) 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
US8097419B2 (en) 2006-09-12 2012-01-17 Longhorn Vaccines & Diagnostics Llc Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
EP1900824A1 (en) * 2006-09-14 2008-03-19 Deutsches Krebsforschungszentrum Stiftung Des Öffentlichen Rechts Gene expression signature for the prognosis, diagnosis and therapy of prostate cancer and uses thereof
JP2010510964A (ja) * 2006-09-19 2010-04-08 アシュラジェン インコーポレイテッド 治療的介入の標的としての、ｍｉＲ−１５、ｍｉＲ−２６、ｍｉＲ−３１、ｍｉＲ−１４５、ｍｉＲ−１４７、ｍｉＲ−１８８、ｍｉＲ−２１５、ｍｉＲ−２１６、ｍｉＲ−３３１、ｍｍｕ−ｍｉＲ−２９２−３ｐによって調節される遺伝子および経路
JP5350257B2 (ja) * 2006-11-02 2013-11-27 ベリデックス・エルエルシー 免疫磁気ｍｒｉ造影剤を用いた活性化された血管内皮の画像化
US9114398B2 (en) 2006-11-29 2015-08-25 Canon U.S. Life Sciences, Inc. Device and method for digital multiplex PCR assays
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
CN101627121A (zh) * 2006-12-08 2010-01-13 奥斯瑞根公司 作为治疗干预的靶标的miRNA调控基因和路径
CA2671194A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-20 regulated genes and pathways as targets for therapeutic intervention
CA2671294A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-21 regulated genes and pathways as targets for therapeutic intervention
US7955802B2 (en) 2006-12-13 2011-06-07 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time
WO2008074101A2 (en) 2006-12-21 2008-06-26 Agriculture Victoria Services Pty Limited Artificial selection method and reagents
US8535888B2 (en) 2006-12-29 2013-09-17 Mayo Foundation For Medical Education And Research Compositions and methods for detecting methicillin-resistant S. aureus
CA2671270A1 (en) * 2006-12-29 2008-07-17 Asuragen, Inc. Mir-16 regulated genes and pathways as targets for therapeutic intervention
EP2126114B1 (en) * 2007-02-01 2016-09-28 Abacus Diagnostica OY Method for detection of presence of target polynucleotide in samples
NZ589179A (en) 2007-02-02 2011-01-28 Genera Biosystems Ltd Generation of nucleic acid molecules using solid phase amplification
US20100190167A1 (en) * 2007-02-14 2010-07-29 Genisphere Inc. Methods, Reagents and Kits for Detection of Nucleic Acid Molecules
JP4942508B2 (ja) * 2007-02-20 2012-05-30 アークレイ株式会社 ａｂｌ遺伝子変異の検出用プローブおよびその用途
US9475051B2 (en) * 2007-02-27 2016-10-25 Sony Corporation Nucleic acid amplifier
GB0703996D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
GB0703997D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
EP3091088B1 (en) 2007-03-28 2019-02-06 Signal Diagnostics System and method for high resolution analysis of nucleic acids to detect sequence variations
JP5191041B2 (ja) * 2007-04-05 2013-04-24 エフ．ホフマン−ラ ロシュ アーゲー 急速ワンステップｒｔ−ｐｃｒ
JP2010524454A (ja) 2007-04-19 2010-07-22 モレキュラー ディテクション インコーポレーテッド 抗生物質耐性細菌の検出および分析のための方法、組成物、およびキット
US20090232893A1 (en) * 2007-05-22 2009-09-17 Bader Andreas G miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
CN101801419A (zh) * 2007-06-08 2010-08-11 米尔纳疗法公司 作为治疗干预的靶标的miR-34调控的基因和路径
DE102007031137A1 (de) * 2007-06-13 2008-12-18 Attomol Gmbh Molekulare Diagnostika Verfahren und Sonden/Primärsystem zum "real time" Nachweis eines Nukleinsäuretargets
EP2167964B1 (de) * 2007-06-13 2015-03-11 Attomol GmbH Molekulare Diagnostika Verfahren zur durchführung und auswertung von mix&measure assays für die messung von reaktionskinetiken sowie von konzentrationen und affinitäten von analyten im multiplexformat
CA2691197C (en) 2007-06-21 2013-03-12 Gen-Probe Incorporated Instrument and receptacles for use in performing processes
US7635566B2 (en) 2007-06-29 2009-12-22 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
US20090023138A1 (en) * 2007-07-17 2009-01-22 Zila Biotechnology, Inc. Oral cancer markers and their detection
US20090055243A1 (en) 2007-08-21 2009-02-26 Jayson Lee Lusk Systems and methods for predicting a livestock marketing method
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US8386184B2 (en) 2007-08-28 2013-02-26 Becton, Dickinson And Company Systems and methods for determining an amount of starting reagent using the polymerase chain reaction
US8380457B2 (en) * 2007-08-29 2013-02-19 Canon U.S. Life Sciences, Inc. Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes
DE102007041864B4 (de) 2007-09-04 2012-05-03 Sirs-Lab Gmbh Verfahren zum Nachweis von Bakterien und Pilzen
EP2198050A1 (en) 2007-09-14 2010-06-23 Asuragen, INC. Micrornas differentially expressed in cervical cancer and uses thereof
US20090119020A1 (en) 2007-09-25 2009-05-07 Roche Molecular Systems, Inc. Pcr elbow determination using quadratic test for curvature analysis of a double sigmoid
BRPI0816047A2 (pt) 2007-09-28 2018-06-05 3M Innovative Properties Co conjunto de oligonucleotídeos para detecção de um ácido nucleico alvo, métodos para detecção da presença ou ausência de um ácido nucleico alvo e de pelo menos dois ácidos nucleicos alvo, kit, mistura de reação e amplicon identificado por fluorescência
US20090186015A1 (en) * 2007-10-18 2009-07-23 Latham Gary J Micrornas differentially expressed in lung diseases and uses thereof
WO2009055050A1 (en) * 2007-10-25 2009-04-30 Canon U.S. Life Sciences, Inc High-resolution melting analysis
FR2922897B1 (fr) * 2007-10-25 2009-11-27 Bio Rad Pasteur Mesure d'une population d'acides nucleiques,en particulier par pcr en temps reel.
CN105349647B (zh) 2007-10-30 2020-08-28 完整基因有限公司 用于核酸高通量测序的方法
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
US20090137058A1 (en) * 2007-11-28 2009-05-28 General Electric Company Chemical and biological detection method and device based on measurements of fluorescence and reflectivity
WO2009070805A2 (en) * 2007-12-01 2009-06-04 Asuragen, Inc. Mir-124 regulated genes and pathways as targets for therapeutic intervention
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
US20090162863A1 (en) * 2007-12-13 2009-06-25 Hitachi High-Technologies Corporation Nucleic acid detection probe
US20090192114A1 (en) * 2007-12-21 2009-07-30 Dmitriy Ovcharenko miR-10 Regulated Genes and Pathways as Targets for Therapeutic Intervention
WO2009081966A1 (ja) * 2007-12-26 2009-07-02 Arkray, Inc. 核酸増幅判定方法および核酸増幅判定装置
CN101663408B (zh) * 2007-12-26 2012-11-28 爱科来株式会社 熔解曲线分析方法以及熔解曲线分析装置
DE102007063102B4 (de) 2007-12-28 2022-02-10 Robert Bosch Gmbh Verfahren zur Erfassung eines periodisch pulsierenden Betriebsparameters
EP2238459B1 (en) * 2008-01-23 2019-05-08 Roche Diagnostics GmbH Integrated instrument performing synthesis and amplification
JP5665548B2 (ja) * 2008-02-08 2015-02-04 メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ クロストリジウム・ディフィシレの検出
WO2009100430A2 (en) * 2008-02-08 2009-08-13 Asuragen, Inc miRNAs DIFFERENTIALLY EXPRESSED IN LYMPH NODES FROM CANCER PATIENTS
WO2009103003A2 (en) * 2008-02-15 2009-08-20 Bio-Rad Laboratories, Inc. Scanning fluorescent reader with diffuser system
WO2009111475A2 (en) 2008-03-03 2009-09-11 Heatflow Technologies, Inc. Heat flow polymerase chain reaction systems and methods
WO2009111072A1 (en) * 2008-03-05 2009-09-11 Clean Earth Technologies, Llc Multiplexed pcr-coupled pna labeled beads flow cytometric assay for simultaneous detection of multiple biological agents
CA2658520C (en) * 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
US20090253780A1 (en) * 2008-03-26 2009-10-08 Fumitaka Takeshita COMPOSITIONS AND METHODS RELATED TO miR-16 AND THERAPY OF PROSTATE CANCER
EP2108699B1 (en) 2008-04-08 2014-06-25 F.Hoffmann-La Roche Ag Analytical processing and detection device
US20090258928A1 (en) * 2008-04-08 2009-10-15 Asuragen, Inc. Methods and compositions for diagnosing and modulating human papillomavirus (hpv)
ATE537272T1 (de) * 2008-04-08 2011-12-15 Hoffmann La Roche Detektion von pcr-produkten in der gelelektrophorese
US8258111B2 (en) * 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
US20130196310A1 (en) 2008-05-20 2013-08-01 Rapid Pathogen Screening, Inc. Method and Device for Combined Detection of Viral and Bacterial Infections
US9068981B2 (en) 2009-12-04 2015-06-30 Rapid Pathogen Screening, Inc. Lateral flow assays with time delayed components
US8815609B2 (en) 2008-05-20 2014-08-26 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with diverting zone
US8962260B2 (en) 2008-05-20 2015-02-24 Rapid Pathogen Screening, Inc. Method and device for combined detection of viral and bacterial infections
US8609433B2 (en) 2009-12-04 2013-12-17 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with sample compressor
ES2439951T3 (es) 2008-06-06 2014-01-27 F. Hoffmann-La Roche Ag Detección y cuantificación multiplex de ácidos nucleicos microbianos controlada de forma interna
US20110086359A1 (en) 2008-06-10 2011-04-14 Rapid Pathogen Screening, Inc. Lateral flow assays
EP2138588A1 (en) * 2008-06-23 2009-12-30 Koninklijke Philips Electronics N.V. Melting curve measurement during amplification
US8097412B2 (en) 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass
DE102008040513B4 (de) * 2008-07-17 2010-08-26 BAM Bundesanstalt für Materialforschung und -prüfung Verwendung einer langwellig emittierenden Cyaninverbindung als NIR-Fluoreszenzstandard und Kit zur Kalibrierung von Photolumineszenzmesssystemen
EP2148187A1 (de) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion
CA2638458A1 (en) * 2008-07-31 2010-01-31 Spartan Bioscience Inc. Thermal recycling by positioning relative to fixed-temperature source
GB0909333D0 (en) 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
CN102112631A (zh) 2008-08-01 2011-06-29 霍夫曼-拉罗奇有限公司 用于mRNA扩增的改进的裂解和逆转录
US20100055733A1 (en) * 2008-09-04 2010-03-04 Lutolf Matthias P Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels
US9250249B2 (en) 2008-09-08 2016-02-02 Enzo Biochem, Inc. Autophagy and phospholipidosis pathway assays
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
US7910720B2 (en) * 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
CN102187215A (zh) 2008-09-09 2011-09-14 生物辐射实验室股份有限公司 基于回归的多级pcr分析系统
US8219324B2 (en) 2008-09-12 2012-07-10 Roche Molecular Systems, Inc. Real-time PCR elbow calling by equation-less algorithm
US9540686B2 (en) * 2008-09-18 2017-01-10 Canon U.S. Life Sciences, Inc. Systems and methods for the amplification of DNA
CA2682439A1 (en) 2008-10-17 2010-04-17 F. Hoffmann-La Roche Ag Cell monitoring and molecular analysis
US8170804B2 (en) * 2008-10-17 2012-05-01 Roche Molecular Systems, Inc. Determination of melting temperatures of DNA
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
US20100119454A1 (en) * 2008-11-03 2010-05-13 Ping Shen Use of the conserved Drosophila NPFR1 system for uncovering interacting genes and pathways important in nociception and stress response
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
EP3699296A1 (en) 2008-11-07 2020-08-26 Adaptive Biotechnologies Corporation Methods of monitoring conditions by sequence analysis
US8691510B2 (en) 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
WO2010056737A2 (en) * 2008-11-11 2010-05-20 Mirna Therapeutics, Inc. Methods and compositions involving mirnas in cancer stem cells
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
WO2010067055A1 (en) 2008-12-09 2010-06-17 Oxitec Ltd. Enhanced taqman probe based amplification
CN102301005A (zh) 2008-12-17 2011-12-28 生命技术公司 用于检测等位基因变体的方法、组合物和试剂盒
CA2688174C (en) 2008-12-19 2018-08-07 F. Hoffmann-La Roche Ag Dry composition of reaction compounds with stabilized polymerase
US20100159452A1 (en) * 2008-12-22 2010-06-24 Roche Molecular Systems, Inc. Method For Detecting a Target Nucleic Acid in a Sample
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
JP5818688B2 (ja) * 2009-01-08 2015-11-18 バイオ−ラッド ラボラトリーズ インコーポレーティッド 核酸増幅反応の効率を改善するための方法および組成物
SG183029A1 (en) 2009-01-13 2012-08-30 Fluidigm Corp Single-cell nucleic acid analysis
DK3059337T3 (da) 2009-01-15 2019-07-22 Adaptive Biotechnologies Corp Adaptive immunity profiling og metoder til frembringelse af monoklonale antibodier
CA2750900C (en) 2009-01-30 2017-03-28 Gen-Probe Incorporated Systems and methods for detecting a signal and applying thermal energy to a signal transmission element
SI2384368T1 (sl) 2009-01-30 2016-04-29 Kantonsspital Aarau Ag Analiza genskega odmerjanja
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US8606527B2 (en) 2009-02-27 2013-12-10 Bio-Rad Laboratories, Inc. SNP detection by melt curve clustering
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
US9542526B2 (en) * 2009-03-10 2017-01-10 Canon U.S. Life Sciences, Inc. Method and system for temperature correction in thermal melt analysis
EP2411543B1 (en) 2009-03-27 2017-04-19 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
GB0905325D0 (en) 2009-03-30 2009-05-13 Selex Sensors & Airborne Sys Detection system
WO2010115160A2 (en) * 2009-04-03 2010-10-07 Helixis, Inc. Devices and methods for heating biological samples
EP2419527B1 (en) 2009-04-07 2013-06-26 Koninklijke Philips Electronics N.V. Method for the detection and characterization of a toxinogenic clostridium difficile strain
CN104630220A (zh) 2009-04-17 2015-05-20 巴斯夫植物科学有限公司 胚乳中有效的植物启动子及其用途
US20120122161A1 (en) 2009-05-22 2012-05-17 Esther Musgrave-Brown Sorting Asymmetrically Tagged Nucleic Acids by Selective Primer Extension
US8691504B2 (en) 2009-05-26 2014-04-08 Xiamen University Method for detecting variations in nucleic acid sequences
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
EP2435461B1 (en) 2009-05-29 2017-08-09 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
SG176691A1 (en) 2009-06-25 2012-01-30 Hutchinson Fred Cancer Res Method of measuring adaptive immunity
EP3018218B1 (en) 2009-07-21 2018-01-24 Gen-Probe Incorporated Methods for detection of nucleic acid sequences over an extended dynamic range
WO2011022589A2 (en) * 2009-08-20 2011-02-24 Mayo Foundation For Medical Education And Research Detection of enterovirus
WO2011021102A2 (en) 2009-08-20 2011-02-24 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
FI20090306A0 (fi) 2009-08-25 2009-08-25 Qret Technologies Oy Erotusvapaa määritysmenetelmä
US8219366B2 (en) 2009-08-26 2012-07-10 Roche Molecular Sytems, Inc. Determination of elbow values for PCR for parabolic shaped curves
CN201837588U (zh) * 2009-09-09 2011-05-18 海利克斯公司 用于多个反应的光学系统
US8815515B1 (en) 2009-09-24 2014-08-26 University Of Utah Research Foundation Methods, compositions, and kits for rare allele detection
US20110111403A1 (en) 2009-10-30 2011-05-12 Life Technologies Corporation Multi-primer assay for mycoplasma detection
US8524450B2 (en) 2009-10-30 2013-09-03 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
US20120245041A1 (en) 2009-11-04 2012-09-27 Sydney Brenner Base-by-base mutation screening
US9133343B2 (en) * 2009-11-30 2015-09-15 Enzo Biochem, Inc. Dyes and compositions, and processes for using same in analysis of protein aggregation and other applications
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
WO2011087789A2 (en) 2009-12-22 2011-07-21 Becton, Dickinson And Company Methods for the detection of microorganisms
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
WO2011086006A1 (en) * 2010-01-15 2011-07-21 Steffen Mergemeier Method for detecting more than one target in a pcr-based approach applying an unspecific dye which is not interfering with the emission of fluorophore-labeled probes
US20110177512A1 (en) * 2010-01-19 2011-07-21 Predictive Biosciences, Inc. Method for assuring amplification of an abnormal nucleic acid in a sample
WO2011101744A2 (en) 2010-02-22 2011-08-25 Population Genetics Technologies Ltd. Region of interest extraction and normalization methods
WO2011107887A2 (en) 2010-03-02 2011-09-09 Population Genetic Technologies Ltd. Methods for replicating polynucleotides with secondary structure
GB201004339D0 (en) 2010-03-16 2010-04-28 Enigma Diagnostics Ltd Sequence detection assay
WO2011122501A1 (ja) * 2010-03-29 2011-10-06 凸版印刷株式会社 標的塩基配列の識別方法
DE102010003781B4 (de) 2010-04-08 2012-08-16 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
US8774494B2 (en) 2010-04-30 2014-07-08 Complete Genomics, Inc. Method and system for accurate alignment and registration of array for DNA sequencing
US20130065790A1 (en) 2010-05-05 2013-03-14 Check-Points Holding B.V. Assays, compositions and methods for detecting drug resistant micro-organisms
CN104673899A (zh) 2010-05-06 2015-06-03 赛昆塔公司 利用克隆型谱监测健康和疾病状态
GB201007867D0 (en) 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Signalling system
GB201007868D0 (en) 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Sequence detection assay
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
EP3290529B1 (en) 2010-06-11 2019-05-22 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
WO2011161549A2 (en) 2010-06-24 2011-12-29 Population Genetics Technologies Ltd. Methods and compositions for polynucleotide library production, immortalization and region of interest extraction
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
DE112011102524T5 (de) 2010-07-29 2013-07-11 Roche Diagnostics Gmbh Präparation generischer Proben
CA2802548C (en) 2010-07-29 2017-01-03 F. Hoffmann-La Roche Ag Qualitative and quantitative detection of microbial nucleic acids
WO2012013734A1 (en) 2010-07-29 2012-02-02 F. Hoffmann - La Roche Ag Control nucleic acids for multiple parameters
WO2012013732A1 (en) 2010-07-29 2012-02-02 F. Hoffmann - La Roche Ag Generic pcr
CN103210079B (zh) 2010-08-02 2015-07-22 B·L·韦特 用于聚合酶链式反应的可增压筒
JP5959516B2 (ja) 2010-08-18 2016-08-02 ライフ テクノロジーズ コーポレーション 電気化学的検出装置のためのマイクロウェルの化学コーティング法
WO2012034130A2 (en) 2010-09-10 2012-03-15 Qiagen Methods and compositions for nucleic acid detection
US20130338020A1 (en) 2010-09-13 2013-12-19 Commonwealth Scientific And Industrial Research Organisation Epigenetic markers of colorectal cancers and diagnostic methods using the same
CN110878345A (zh) 2010-09-21 2020-03-13 安捷伦科技有限公司 通过分子计数提高等位基因调用的置信度
WO2012038049A2 (en) 2010-09-22 2012-03-29 Roche Diagnostics Gmbh Amplification of distant nucleic acid targets using engineered primers
EP2625285B1 (en) 2010-10-04 2020-04-29 Roche Diagniostics GmbH Method for cell lysis and pcr within the same reaction vessel
WO2012045668A1 (en) 2010-10-04 2012-04-12 Roche Diagnostics Gmbh Method for cell lysis in a rt-pcr reaction buffer
EP2625286B1 (en) 2010-10-04 2020-04-15 Roche Diagniostics GmbH Method for cell lysis in a pcr reaction buffer
GB201017011D0 (en) 2010-10-08 2010-11-24 Skova Services Ltd Assay
EP3561073A1 (en) 2010-10-08 2019-10-30 President and Fellows of Harvard College High-throughput immune sequencing
EP2625320B1 (en) 2010-10-08 2019-03-27 President and Fellows of Harvard College High-throughput single cell barcoding
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
KR101420094B1 (ko) 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량ｐｃｒ을 이용한 병원균의 전자동 생균수검사방법, 정량면역ｐｃｒ을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
SG10201407036YA (en) 2010-11-05 2014-12-30 Transbio Ltd Markers of Endothelial Progenitor Cells and Uses Thereof
RU2451086C1 (ru) * 2010-12-03 2012-05-20 Государственное образовательное учреждение высшего профессионального образования Смоленская государственная медицинская академия федерального агентства по здравоохранению и социальному развитию Способ детекции специфических нуклеотидных последовательностей и нуклеотидных замен с помощью пцр в режиме реального времени с эффектом гашения флуоресценции зонда праймером
US8569254B2 (en) 2010-12-10 2013-10-29 National Yang Ming University Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same
EP2465945A1 (en) 2010-12-17 2012-06-20 F. Hoffmann-La Roche AG Generic matrix for control nucleic acids
WO2012087135A1 (en) 2010-12-22 2012-06-28 Academisch Ziekenhuis Leiden H.O.D.N. Lumc Genetic markers specific for clostridium difficile ribotypes 027 (nap01/b1; rt 027) and 078 (nap7/8; rt 078) and their use
WO2012118555A1 (en) 2010-12-29 2012-09-07 Life Technologies Corporation Time-warped background signal for sequencing-by-synthesis operations
US10146906B2 (en) 2010-12-30 2018-12-04 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
EP2663652B1 (en) 2011-01-11 2018-11-14 Roche Diagniostics GmbH High resolution melting analysis as a prescreening tool
MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
US9096892B1 (en) 2011-02-04 2015-08-04 K2 Biomicrosystems, LLC Nucleic acid amplification and monitoring apparatus
US9365897B2 (en) 2011-02-08 2016-06-14 Illumina, Inc. Selective enrichment of nucleic acids
FR2971257B1 (fr) 2011-02-09 2020-12-04 Bio Rad Pasteur Combinaison de biomarqueurs pour le pronostic d'une reponse ou non-reponse a un traitement anti-vhc
FR2971258B1 (fr) 2011-02-09 2020-12-04 Bio Rad Pasteur Combinaison de biomarqueurs pour le pronostic d'une reponse ou non-reponse a un traitement anti-vhc
FR2971256B1 (fr) 2011-02-09 2024-09-27 Bio Rad Pasteur Combinaison de biomarqueurs pour la detection et l'evaluation d'une fibrose hepatique
ES2827293T3 (es) 2011-03-08 2021-05-20 Univ Laval Dispositivo centrípeto fluídico
EP3878975A1 (en) 2011-04-08 2021-09-15 Life Technologies Corporation Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
CN106148512B (zh) 2011-04-15 2020-07-10 贝克顿·迪金森公司 扫描实时微流体热循环仪和用于同步的热循环和扫描光学检测的方法
WO2012142656A1 (en) 2011-04-18 2012-10-26 Garvan Institute Of Medical Research Method of diagnosing cancer
KR102252447B1 (ko) * 2011-05-04 2021-05-17 바이오셉트 인코포레이티드 핵산 서열 변이체를 검출하는 방법
CN103635594B (zh) 2011-05-09 2018-02-13 富鲁达公司 基于探针的核酸检测
WO2012153153A1 (en) 2011-05-11 2012-11-15 Diagon Kft. Procedure for rapid determination of viruses using nucleic acid-based molecular diagnostics, and a kit for this purpose
WO2012159025A2 (en) 2011-05-18 2012-11-22 Life Technologies Corporation Chromosome conformation analysis
DK2801626T3 (en) 2011-05-24 2016-06-20 Elitechgroup B V Detection of methicillin-resistant Staphylococcus aureus.
US9034581B2 (en) 2011-05-26 2015-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Staphylococcus aureus
WO2012166913A1 (en) * 2011-06-01 2012-12-06 Streck, Inc. Rapid thermocycler system for rapid amplification of nucleic acids and related methods
WO2012171997A1 (en) 2011-06-14 2012-12-20 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for determining the expression level of a gene of interest including correction of rt-qpcr data for genomic dna-derived signals
EP4324935B1 (en) 2011-08-18 2025-02-19 Life Technologies Corporation Methods, systems and computer readable media for making base calls in nucleic acid sequencing
HK1199066A1 (en) 2011-08-18 2015-06-19 Nestec S.A. Compositions and methods for detecting allelic variants
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
EP2756101B1 (en) 2011-09-15 2018-05-23 David A. Shafer Probe: antiprobe compositions for high specificity dna or rna detection
EP3263718A1 (en) 2011-09-16 2018-01-03 Lexogen GmbH Nucleic acid transcription method
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
CA2847943C (en) 2011-09-23 2017-08-29 F.Hoffmann-La Roche Ag Use of g-clamp for improved allele-specific pcr
EP2768607B1 (en) 2011-09-26 2021-08-18 Thermo Fisher Scientific GENEART GmbH Multiwell plate for high efficiency, small volume nucleic acid synthesis
EP2761032B1 (en) 2011-09-29 2017-07-19 Luminex Corporation Hydrolysis probes
US9416153B2 (en) 2011-10-11 2016-08-16 Enzo Life Sciences, Inc. Fluorescent dyes
EP2766711B1 (en) * 2011-10-14 2017-12-13 Becton, Dickinson and Company Square wave thermal cycling
EP2766498B1 (en) 2011-10-14 2019-06-19 President and Fellows of Harvard College Sequencing by structure assembly
HK1201565A1 (en) 2011-10-21 2015-09-04 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
US9447458B2 (en) 2011-11-16 2016-09-20 Canon U.S. Life Sciences, Inc. Detection of neighboring variants
US9260714B2 (en) 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
AU2012347460B2 (en) 2011-12-09 2017-05-25 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
US20140377762A1 (en) 2011-12-19 2014-12-25 360 Genomics Ltd. Method for enriching and detection of variant target nucleic acids
US8981318B1 (en) 2011-12-30 2015-03-17 Gene Capture, Inc. Multi-dimensional scanner for nano-second time scale signal detection
US9194840B2 (en) 2012-01-19 2015-11-24 Life Technologies Corporation Sensor arrays and methods for making same
WO2013112458A1 (en) 2012-01-24 2013-08-01 Beth Israel Deaconess Medical Center, Inc. Novel chrebp isoforms and methods using the same
US9864846B2 (en) 2012-01-31 2018-01-09 Life Technologies Corporation Methods and computer program products for compression of sequencing data
US9515676B2 (en) 2012-01-31 2016-12-06 Life Technologies Corporation Methods and computer program products for compression of sequencing data
WO2013113748A1 (en) * 2012-02-02 2013-08-08 Primer Design Ltd Method for detecting and genotyping target nucleic acid
KR101912401B1 (ko) 2012-02-03 2018-10-26 캘리포니아 인스티튜트 오브 테크놀로지 다중화된 생화학적 분석에서의 시그널 인코딩 및 디코딩
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
WO2013124743A1 (en) 2012-02-22 2013-08-29 Population Genetics Technologies Ltd. Compositions and methods for intramolecular nucleic acid rearrangement ii
EP2820155B1 (en) 2012-02-28 2017-07-26 Population Genetics Technologies Ltd. Method for attaching a counter sequence to a nucleic acid sample
JP6302847B2 (ja) 2012-03-05 2018-03-28 アダプティヴ バイオテクノロジーズ コーポレーション 頻度が一致したサブユニットからの、対をなす免疫受容体鎖の決定
US9650665B2 (en) 2012-03-05 2017-05-16 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence by PTO cleavage and extension assay
EP2828400B1 (en) 2012-03-21 2018-09-19 Roche Diagniostics GmbH Methods for assessing rna quality
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
EP2831276B1 (en) 2012-05-08 2016-04-20 Adaptive Biotechnologies Corporation Compositions and method for measuring and calibrating amplification bias in multiplexed pcr reactions
CA3152533C (en) 2012-05-11 2023-10-17 Commonwealth Scientific And Industrial Research Organisation Diagnostic gene marker panel for colorectal cancer
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US10941449B2 (en) 2012-05-18 2021-03-09 Clinical Genomics Pty. Ltd. Method of screening for colorectal cancer
EP2855704A4 (en) * 2012-05-25 2016-03-16 Accugenomics Inc NUCLEIC ACID AMPLIFICATION AND USE THEREOF
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
US9488823B2 (en) 2012-06-07 2016-11-08 Complete Genomics, Inc. Techniques for scanned illumination
US9628676B2 (en) 2012-06-07 2017-04-18 Complete Genomics, Inc. Imaging systems with movable scan mirrors
KR20230074845A (ko) 2012-06-14 2023-05-31 라이프 테크놀로지스 코포레이션 폴리머라제 연쇄 반응 (pcr)을 위한 신규 조성물, 방법 및 키트
US9384320B2 (en) 2012-07-19 2016-07-05 President And Fellows Of Harvard College Methods of storing information using nucleic acids
EP2877952B1 (en) 2012-07-27 2023-04-12 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
WO2014022827A1 (en) 2012-08-03 2014-02-06 California Institute Of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
US9957557B2 (en) 2012-08-13 2018-05-01 Life Genetics Lab, Llc Development of a highly sensitive quantification system for assessing DNA degradation and quality in forensic samples
US10993418B2 (en) 2012-08-13 2021-05-04 Life Genetics Lab, Llc Method for measuring tumor burden in patient derived xenograft (PDX) mice
US9982313B2 (en) 2012-08-17 2018-05-29 Roche Molecular Systems, Inc. Compositions and methods for detection of herpes simplex virus 1 and 2
ES2887939T3 (es) 2012-09-10 2021-12-29 Biofire Diagnostics Llc Detección de ciclos de amplificación múltiples
US9914968B2 (en) 2012-09-26 2018-03-13 Cepheid Honeycomb tube
JP6449160B2 (ja) 2012-10-01 2019-01-09 アダプティブ バイオテクノロジーズ コーポレイション 適応免疫受容体多様性およびクローン性特性決定による免疫能力評価関連出願の相互参照
US10176293B2 (en) 2012-10-02 2019-01-08 Roche Molecular Systems, Inc. Universal method to determine real-time PCR cycle threshold values
EP3379245A1 (en) * 2012-10-09 2018-09-26 University Of Utah Research Foundation Monitoring temperature with fluorescence
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
EP2722399A1 (en) 2012-10-18 2014-04-23 Roche Diagniostics GmbH Method for preventing high molecular weight products during amplification
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
CN103114131B (zh) 2012-11-30 2018-10-02 珠海市坤元农业科技有限公司 一种引物中部序列干扰pcr技术
CN108624668B (zh) 2013-02-01 2022-12-02 加利福尼亚大学董事会 用于基因组组装及单体型定相的方法
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
EP2770065B1 (en) 2013-02-25 2017-12-13 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
WO2014142575A1 (en) 2013-03-13 2014-09-18 Seegene, Inc. Quantification of target nucleic acid using melting peak analysis
US10563240B2 (en) 2013-03-14 2020-02-18 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
AU2013202788B2 (en) * 2013-03-14 2015-10-01 Gen-Probe Incorporated Indexing signal detection module
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
JP6169245B2 (ja) 2013-03-15 2017-07-26 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ 後部アクセス可能トラックシステムを有する自動診断分析装置および関連方法
US20140273181A1 (en) 2013-03-15 2014-09-18 Biofire Diagnostics, Inc. Compact optical system for substantially simultaneous monitoring of samples in a sample array
EP2925895B1 (en) 2013-03-15 2018-11-28 Avellino Lab USA, Inc. Methods for improved isolation of genomic dna templates for allele detection
ES2970108T3 (es) 2013-03-15 2024-05-27 Abbott Lab Analizadores de diagnóstico con carruseles de pretratamiento y métodos relacionados
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
CN109358202B (zh) 2013-03-15 2023-04-07 雅培制药有限公司 具有竖直布置的圆盘传送带的自动化诊断分析仪及相关方法
WO2014144495A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
EP2976435B1 (en) 2013-03-19 2017-10-25 Directed Genomics, LLC Enrichment of target sequences
CN105492624B (zh) 2013-04-23 2019-10-18 恩姆菲舍尔科技公司 熔解曲线分析
CA2912312C (en) 2013-05-20 2023-01-24 Board Of Trustees Of The University Of Arkansas Gep5 model for multiple myeloma
WO2014205083A1 (en) 2013-06-19 2014-12-24 Luminex Corporation Real-time multiplexed hydrolysis probe assay using spectrally identifiable microspheres
CN111349692B (zh) 2013-06-25 2023-10-13 C.威特沃 进行聚合酶链式反应的方法及其相关应用
WO2014210593A1 (en) 2013-06-28 2014-12-31 Streck, Inc. Devices for real-time polymerase chain reaction
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
KR101863943B1 (ko) 2013-07-15 2018-06-01 주식회사 씨젠 Ｐｔｏ 절단 및 연장-의존적 고정화 올리고뉴클레오타이드 혼성화를 이용한 타겟 핵산 서열의 검출
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
EP3461913B1 (en) 2013-08-09 2020-06-24 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
US20160186265A1 (en) 2013-08-15 2016-06-30 Centre Hospitalier Universitarie Vaudois Methods for Typing HLA Alleles
US10410739B2 (en) 2013-10-04 2019-09-10 Life Technologies Corporation Methods and systems for modeling phasing effects in sequencing using termination chemistry
AU2014331828B2 (en) 2013-10-09 2020-05-14 Fluoresentric, Inc. Multiplex probes
US10072288B2 (en) 2013-11-11 2018-09-11 Roche Molecular Systems, Inc. Detecting single nucleotide polymorphism using overlapped primer and melting probe
EP3872192A1 (en) 2013-11-15 2021-09-01 Avellino Lab USA, Inc. Methods for multiplex detection of alleles associated with ophthalmic conditions
ES2693217T3 (es) 2013-12-02 2018-12-10 Personal Genome Diagnostics Inc. Método para evaluar variantes minoritarias en una muestra
US9476853B2 (en) 2013-12-10 2016-10-25 Life Technologies Corporation System and method for forming microwells
WO2015089243A1 (en) 2013-12-11 2015-06-18 The Regents For Of The University Of California Methods for labeling dna fragments to recontruct physical linkage and phase
JP2017506080A (ja) 2014-02-21 2017-03-02 プレジデント アンド フェローズ オブ ハーバード カレッジ アロステリックタンパク質の新規設計
US20170292149A1 (en) 2014-03-05 2017-10-12 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US10195610B2 (en) 2014-03-10 2019-02-05 Click Diagnostics, Inc. Cartridge-based thermocycler
EP2921556A1 (en) 2014-03-21 2015-09-23 Lexogen GmbH Copy number preserving RNA analysis method
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
EP3792366A1 (en) 2014-04-04 2021-03-17 Affymetrix, Inc. Improved compositions and methods for molecular inversion probe assays
BE1022048B1 (nl) * 2014-04-11 2016-02-10 Ugentec Bvba Methoden voor fluorescentie data correctie
AU2015274693B2 (en) 2014-06-10 2019-01-03 Reolab, Inc. Discrete elements for 3D microfluidics
DE102014212657B4 (de) * 2014-06-30 2016-03-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. System und Verfahren zur bedarfsgerechten Zuführung von Beleuchtungsenergie an Pflanzen
GB201411567D0 (en) * 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
WO2016019360A1 (en) 2014-08-01 2016-02-04 Dovetail Genomics Llc Tagging nucleic acids for sequence assembly
DE102014215352A1 (de) * 2014-08-04 2016-02-04 Berthold Technologies Gmbh & Co. Kg Messgerät und Verfahren zum Bestimmen einer Messgröße
WO2016025452A1 (en) 2014-08-11 2016-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acids assays
NZ730044A (en) 2014-09-15 2024-08-30 Abvitro Llc High-throughput nucleotide library sequencing
US11352672B2 (en) 2014-09-15 2022-06-07 Garvan Institute Of Medical Research Methods for diagnosis, prognosis and monitoring of breast cancer and reagents therefor
HUE055583T2 (hu) * 2014-09-16 2021-12-28 Sangamo Therapeutics Inc Eljárások és készítmények nukleáz által közvetített genommódosításhoz és -javításhoz hematopoetikus õssejtekben
WO2016049694A1 (en) 2014-09-30 2016-04-07 Genetic Technologies Limited Methods for assessing risk of developing breast cancer
WO2016060974A1 (en) 2014-10-13 2016-04-21 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
US10392663B2 (en) 2014-10-29 2019-08-27 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from a large number of samples
CA2967036A1 (en) 2014-11-13 2016-05-19 The Board Of Trustees Of The University Of Illinois Bio-engineered hyper-functional "super" helicases
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
US9745618B2 (en) 2014-11-19 2017-08-29 Roche Molecular Systems, Inc. Photoblocked probes and methods for sequential detection of nucleic acids
EP3498866A1 (en) 2014-11-25 2019-06-19 Adaptive Biotechnologies Corp. Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
US9920381B2 (en) 2014-12-02 2018-03-20 Roche Molecular Systems, Inc. Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus
CN115927293A (zh) 2014-12-09 2023-04-07 生命技术公司 高效小体积核酸合成
WO2016093838A1 (en) 2014-12-11 2016-06-16 New England Biolabs, Inc. Enrichment of target sequences
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
ES2898103T3 (es) 2014-12-31 2022-03-03 Visby Medical Inc Dispositivos para pruebas de diagnóstico molecular
US10421993B2 (en) 2015-02-11 2019-09-24 Paragon Genomics, Inc. Methods and compositions for reducing non-specific amplification products
JP6777966B2 (ja) 2015-02-17 2020-10-28 ダブテイル ゲノミクス エルエルシー 核酸配列アセンブリ
ES3002665T3 (en) 2015-02-24 2025-03-07 Adaptive Biotechnologies Corp Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
GB2554572B (en) 2015-03-26 2021-06-23 Dovetail Genomics Llc Physical linkage preservation in DNA storage
US9957393B2 (en) 2015-03-30 2018-05-01 Enzo Biochem, Inc. Monoazo dyes with cyclic amine as fluorescence quenchers
AU2016242967B2 (en) 2015-04-01 2021-07-01 Adaptive Biotechnologies Corp. Method of identifying human compatible T cell receptors specific for an antigenic target
CA2983819A1 (en) 2015-04-24 2016-10-27 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
US10968475B2 (en) 2015-05-01 2021-04-06 Vanderbilt University Monitoring and analysis of nucleic acid hybridization state and amplification using L-DNA
EP4220645A3 (en) 2015-05-14 2023-11-08 Life Technologies Corporation Barcode sequences, and related systems and methods
CN107849617B (zh) 2015-06-04 2021-11-02 美之贺美蒂股份有限公司 复数个标的核酸的检测套组及使用其的检测方法
JP2018525305A (ja) * 2015-06-08 2018-09-06 ザ・ユニバーシティ・オブ・シカゴＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃｈｉｃａｇｏ 単分散貴金属ナノ結晶の双角錐鋳型合成
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
EP3353326B1 (en) 2015-09-24 2022-08-24 AbVitro LLC Affinity-oligonucleotide conjugates and uses thereof
EP3353550A1 (en) 2015-09-25 2018-08-01 AbVitro LLC High throughput process for t cell receptor target identification of natively-paired t cell receptor sequences
US11266730B2 (en) 2015-09-29 2022-03-08 The General Hospital Corporation Methods of treating and diagnosing disease using biomarkers for BCG therapy
JP7300831B2 (ja) 2015-10-19 2023-06-30 ダブテイル ゲノミクス エルエルシー ゲノムアセンブリ、ハプロタイプフェージング、および標的に依存しない核酸検出のための方法
US10808287B2 (en) 2015-10-23 2020-10-20 Rapid Pathogen Screening, Inc. Methods and devices for accurate diagnosis of infections
USD799715S1 (en) 2015-10-23 2017-10-10 Gene POC, Inc. Fluidic centripetal device
JP6929865B2 (ja) 2015-11-13 2021-09-01 タラ ムーア 角膜ジストロフィーの治療方法
EP3387107B1 (en) 2015-12-11 2020-08-12 Spartan Bioscience Inc. Tube sealing system and methods for nucleic acid amplification
SG10202111007PA (en) 2015-12-18 2021-11-29 Biofire Defense Llc Solid fluorescence standard
AU2017212152B2 (en) 2016-01-28 2019-07-11 The University Of Melbourne Methods for assessing risk of developing colorectal cancer
KR102744506B1 (ko) 2016-02-23 2024-12-18 더브테일 제노믹스 엘엘씨 게놈 어셈블리를 위한 페이징된 판독 세트의 생성 및 반수체형 페이징
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
US11369965B2 (en) 2016-03-10 2022-06-28 Pioneer Hi-Bred International, Inc. Light-mediated polymerase chain reaction amplification and product detection system and methods of use
CA3017265C (en) 2016-03-11 2022-08-16 F. Hoffmann-La Roche Ag Compositions and methods for detection of zika virus
US10987674B2 (en) 2016-04-22 2021-04-27 Visby Medical, Inc. Printed circuit board heater for an amplification module
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
SG11201810088SA (en) 2016-05-13 2018-12-28 Dovetail Genomics Llc Recovering long-range linkage information from preserved samples
ES2929367T3 (es) 2016-05-18 2022-11-28 Hoffmann La Roche Amplificación por PCR ultrarrápida cuantitativa usando un dispositivo basado en electrohumectación
WO2017202894A1 (en) 2016-05-27 2017-11-30 Roche Diagnostics Gmbh Compositions and methods for detection of trichomonas vaginalis
US10612101B2 (en) 2016-05-27 2020-04-07 Roche Molecular Systems, Inc. Compositions and methods for detection of Mycoplasma genitalium
CN109643578B (zh) 2016-06-01 2023-07-21 生命科技股份有限公司 用于设计基因组合的方法和系统
US20170356025A1 (en) 2016-06-14 2017-12-14 Roche Molecular Systems, Inc. Internal control probes for improving pcr assay performance
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
RU2691763C2 (ru) * 2016-06-21 2019-06-18 Федеральное государственное бюджетное учреждение науки Институт аналитического приборостроения Российской академии наук (ИАП РАН) Устройство для одновременного контроля в реальном масштабе времени множества амплификаций нуклеиновой кислоты
WO2018005710A1 (en) 2016-06-29 2018-01-04 Click Diagnostics, Inc. Devices and methods for the detection of molecules using a flow cell
US10626454B2 (en) 2016-07-27 2020-04-21 SeqMatic, LLC Compositions and methods for nucleic acid amplification and analysis
AU2017301881A1 (en) 2016-07-29 2019-02-07 Juno Therapeutics, Inc. Methods for assessing the presence or absence of replication competent virus
WO2018024562A1 (en) 2016-08-02 2018-02-08 Roche Diagnostics Gmbh Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids
CA3033250C (en) 2016-09-15 2023-08-15 F. Hoffmann-La Roche Ag Methods for performing multiplexed real-time pcr
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US10487358B2 (en) 2016-09-23 2019-11-26 Grail, Inc. Methods of preparing and analyzing cell-free nucleic acid sequencing libraries
AU2017332495A1 (en) 2016-09-24 2019-04-11 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
US9851345B1 (en) 2016-10-12 2017-12-26 Viasphere, Llc Compositions and methods for disease diagnosis using single cell analysis
WO2018073872A1 (ja) 2016-10-17 2018-04-26 オリンパス株式会社 標的核酸分子の検出方法
CN109906276A (zh) 2016-11-07 2019-06-18 格里尔公司 用于检测早期癌症中体细胞突变特征的识别方法
US10793923B2 (en) 2016-11-09 2020-10-06 Roche Molecular Systems, Inc. Compositions and methods for detection of BK virus
JP2020503857A (ja) 2016-12-12 2020-02-06 セファイド 自動反応カートリッジにおける統合化イムノｐｃｒ及び核酸分析
WO2018111872A1 (en) 2016-12-12 2018-06-21 Grail, Inc. Methods for tagging and amplifying rna template molecules for preparing sequencing libraries
US20190211377A1 (en) 2016-12-22 2019-07-11 Roche Molecular Systems, Inc. Cobra probes to detect a marker for epidemic ribotypes of clostridium difficile
US10982351B2 (en) 2016-12-23 2021-04-20 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
US10793901B2 (en) 2016-12-28 2020-10-06 Roche Molecular Systems, Inc. Reversibly protected nucleotide reagents with high thermal stability
US10851410B2 (en) 2017-01-04 2020-12-01 Mgi Tech Co., Ltd. Nucleic acid sequencing using affinity reagents
EP3568493B1 (en) 2017-01-10 2021-03-24 Paragon Genomics, Inc. Methods and compositions for reducing redundant molecular barcodes created in primer extension reactions
EP4212634A1 (en) 2017-03-24 2023-07-19 Gen-Probe Incorporated Compositions and methods for detection of flu b in samples
WO2018175715A1 (en) 2017-03-24 2018-09-27 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Herpes simplex virus type-1(hsv-1) vaccine strain vc2 generating an anti-ehv-1 immune response
US11274344B2 (en) 2017-03-30 2022-03-15 Grail, Inc. Enhanced ligation in sequencing library preparation
WO2018183942A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification
US11118222B2 (en) 2017-03-31 2021-09-14 Grail, Inc. Higher target capture efficiency using probe extension
CA3061620A1 (en) 2017-04-28 2018-11-01 Avellino Lab Usa, Inc. Methods for detecting alleles associated with keratoconus
WO2018213803A1 (en) 2017-05-19 2018-11-22 Neon Therapeutics, Inc. Immunogenic neoantigen identification
US20190093155A1 (en) 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
AU2018273999A1 (en) 2017-05-26 2019-12-05 Abvitro Llc High-throughput polynucleotide library sequencing and transcriptome analysis
US20180363044A1 (en) 2017-06-14 2018-12-20 Roche Molecular Systems, Inc. Compositions and methods for improving the thermal stability of nucleic acid amplification reagents
US11450121B2 (en) 2017-06-27 2022-09-20 The Regents Of The University Of California Label-free digital brightfield analysis of nucleic acid amplification
AU2018291032A1 (en) 2017-06-29 2020-01-16 Juno Therapeutics, Inc. Mouse model for assessing toxicities associated with immunotherapies
CA3156539A1 (en) 2017-07-10 2019-01-17 Gen-Probe Incorporated Analytical systems and methods for nucleic acid amplification using sample assigning parameters
US10760137B2 (en) 2017-07-18 2020-09-01 Roche Molecular Systems, Inc. Compositions and methods for detection of Babesia
EP3661528B1 (en) 2017-07-29 2025-01-15 Juno Therapeutics, Inc. Reagents for expanding cells expressing recombinant receptors
WO2019045532A2 (en) 2017-08-31 2019-03-07 Seegene, Inc. EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS
WO2019046832A1 (en) 2017-09-01 2019-03-07 Juno Therapeutics, Inc. GENE EXPRESSION AND EVALUATION OF RISK OF DEVELOPMENT OF TOXICITY FOLLOWING CELL THERAPY
WO2019055819A1 (en) 2017-09-14 2019-03-21 Grail, Inc. METHODS FOR PREPARING A SEQUENCING LIBRARY FROM SINGLE STRANDED DNA
EP4269583A3 (en) 2017-09-28 2024-01-17 Grail, LLC Enrichment of short nucleic acid fragments in sequencing library preparation
WO2019066461A2 (en) 2017-09-29 2019-04-04 Seegene, Inc. DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO
WO2019063661A1 (en) 2017-09-29 2019-04-04 Roche Diagnostics Gmbh COMPOSITIONS AND METHODS FOR THE DETECTION OF TRICHOMONAS VAGINALIS
WO2019084055A1 (en) 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS
KR20200097253A (ko) 2017-11-01 2020-08-18 주노 쎄러퓨티크스 인코퍼레이티드 T 세포 조성물의 생산방법
AU2018364741B2 (en) 2017-11-09 2021-03-25 Visby Medical, Inc. Portable molecular diagnostic device and methods for the detection of target viruses
US11078534B2 (en) 2017-11-27 2021-08-03 Roche Molecular Systems, Inc. Photocleavable nucleotide reagents with high stability
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019118925A1 (en) 2017-12-15 2019-06-20 Grail, Inc. Methods for enriching for duplex reads in sequencing and error correction
US20190237161A1 (en) 2017-12-22 2019-08-01 Grail, Inc. Error removal using improved library preparation methods
MX2020006923A (es) 2018-01-05 2020-09-09 Seegene Inc Metodo para determinar la presencia o ausencia de m. tuberculosis, m. bovis y m. bovis bcg en una muestra.
EP4502180A3 (en) 2018-01-29 2025-04-30 Gen-Probe Incorporated Analytical systems and methods
US12378592B2 (en) 2018-01-31 2025-08-05 Dovetail Genomics, Llc. Sample prep for DNA linkage recovery
US11535903B2 (en) 2018-01-31 2022-12-27 Juno Therapeutics, Inc. Methods and reagents for assessing the presence or absence of replication competent virus
US20190292596A1 (en) 2018-03-21 2019-09-26 Roche Molecular Systems, Inc. Modified nucleoside phosphates with high thermal stability
EP3781710A4 (en) 2018-04-17 2022-01-05 Chromacode, Inc. METHODS AND SYSTEMS FOR MULTIPLEX ANALYSIS
US11384376B2 (en) 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
JP7470095B2 (ja) 2018-07-10 2024-04-17 ジェン－プローブ・インコーポレーテッド 核酸を検出および定量するための方法およびシステム
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
WO2020028194A1 (en) 2018-07-30 2020-02-06 Readcoor, Inc. Methods and systems for sample processing or analysis
WO2020037290A1 (en) 2018-08-16 2020-02-20 Life Technologies Corporation Reagents, mixtures, kits and methods for amplification of nucleic acids
AU2019337088A1 (en) 2018-09-03 2021-05-06 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
JP7221491B2 (ja) 2018-09-18 2023-02-14 株式会社ミズホメディー 複数の標的核酸を検出するキットを用いる検出方法
EP3874059B1 (en) 2018-10-29 2025-08-20 Koninklijke Nederlandse Akademie van Wetenschappen Single cell full length rna sequencing
CA3117720A1 (en) 2018-11-06 2020-05-14 Juno Therapeutics, Inc. Process for producing genetically engineered t cells
EP3891302B1 (en) 2018-12-03 2025-04-16 F. Hoffmann-La Roche AG Compositions and methods for detection of candida auris
AU2019398307A1 (en) 2018-12-13 2021-06-10 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
CA3121923A1 (en) 2018-12-18 2020-06-25 Wenying Pan Methods for detecting disease using analysis of rna
JP7491934B2 (ja) 2019-01-03 2024-05-28 ディーエヌエー スクリプト オリゴヌクレオチドセットのワンポット合成
EP3937780A4 (en) 2019-03-14 2022-12-07 InSilixa, Inc. METHODS AND SYSTEMS FOR TIMED FLUORESCENCE-BASED DETECTION
MX2021012607A (es) 2019-04-17 2022-03-11 Alpine Immune Sciences Inc Metodos y usos de proteinas de fusion de ligando icos (icosl) variante.
MX2021012888A (es) 2019-04-28 2021-11-17 Visby Medical Inc Dispositivos de diagnostico molecular con capacidad de deteccion digital y conectividad inalambrica.
CN116786186A (zh) * 2019-05-01 2023-09-22 卢米耐克斯公司 用于热循环的装置以及样品模块
CN113785073A (zh) 2019-05-02 2021-12-10 豪夫迈·罗氏有限公司 基于链分离温度利用dITP进行RNA靶标相比于DNA靶标的优先性/选择性扩增
JP7592027B2 (ja) 2019-05-07 2024-11-29 エフ． ホフマン－ラ ロシュ アーゲー ナイセリア・ゴノレア（Ｎｅｉｓｓｅｒｉａ Ｇｏｎｏｒｒｏｈｅａｅ）を検出するための組成物および方法
JP2022537700A (ja) 2019-06-12 2022-08-29 ジュノー セラピューティクス インコーポレイテッド 細胞媒介細胞傷害性療法と生存促進性ｂｃｌ２ファミリータンパク質の阻害剤との併用療法
JP7653924B2 (ja) 2019-06-27 2025-03-31 ダブテイル ゲノミクス エルエルシー 近接ライゲーションのための方法および組成物
EP4004240B1 (en) 2019-07-25 2024-03-13 F. Hoffmann-La Roche AG Compositions and methods for detection of epstein barr virus (ebv)
JP2022545467A (ja) 2019-08-22 2022-10-27 ジュノー セラピューティクス インコーポレイテッド Ｔ細胞療法とｚｅｓｔｅホモログ２エンハンサー（ＥＺＨ２）阻害剤との併用療法および関連方法
ES3039650T3 (en) 2019-08-27 2025-10-23 Hoffmann La Roche Compositions and methods for amplification and detection of hepatitis b virus rna, including hbv rna transcribed from cccdna
US11441167B2 (en) 2019-11-20 2022-09-13 Roche Molecular Systems, Inc. Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi
US20230050863A1 (en) 2019-12-09 2023-02-16 Roche Molecular Systems, Inc. Dicationic fluorescent dyes
JP7716408B2 (ja) 2019-12-27 2025-07-31 エフ． ホフマン－ラ ロシュ アーゲー メチシリン耐性黄色ブドウ球菌を検出するための組成物および方法
EP4085149A4 (en) 2020-01-03 2024-03-06 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
WO2021140173A1 (en) 2020-01-10 2021-07-15 Biouniversa S.R.L. Methods and uses for treating fibrotic solid tumors with bags inhibitors
EP4090734B1 (en) 2020-01-13 2024-11-06 Aspen Neuroscience, Inc. Method of differentiating neural cells and related compositions and methods of use
JP2023516789A (ja) 2020-03-09 2023-04-20 ヤンセン バイオテツク，インコーポレーテツド 組換えベクター核酸の統合を定量化するための組成物及び方法
CN116171333A (zh) 2020-03-09 2023-05-26 豪夫迈·罗氏有限公司 用于检测严重急性呼吸综合征冠状病毒2(sars-cov-2)、甲型流感和乙型流感的组合物和方法
EP4121554B1 (en) 2020-03-18 2024-10-30 MGI Tech Co., Ltd. Restoring phase in massively parallel sequencing
EP4150057A2 (en) 2020-05-13 2023-03-22 Juno Therapeutics, Inc. Process for producing donor-batched cells expressing a recombinant receptor
US10941453B1 (en) 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
IL298097A (en) 2020-05-27 2023-01-01 Genetic Tech Limited Methods of assessing risk of developing a severe response to coronavirus infection
JP2023531531A (ja) 2020-06-26 2023-07-24 ジュノ セラピューティクス ゲーエムベーハー 組換え受容体を条件付きで発現する操作されたｔ細胞、関連ポリヌクレオチド、および方法
US20230129884A1 (en) * 2020-07-20 2023-04-27 Photothermal Spectroscopy Corp. Fluorescence enhanced photothermal infrared spectroscopy and confocal fluorescence imaging
EP4237573A1 (en) 2020-10-29 2023-09-06 DNA Script Enzymatic synthesis of polynucleotide probes
CN112461806B (zh) * 2020-11-18 2022-02-11 厦门大学 基于智能手机的荧光光谱检测方法
JP2023552546A (ja) 2020-12-04 2023-12-18 エフ． ホフマン－ラ ロシュ アーゲー マラリアを検出するための組成物および方法
JP7209980B2 (ja) 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
WO2022136477A1 (en) 2020-12-22 2022-06-30 F. Hoffmann-La Roche Ag Methods for performing multiplexed real-time pcr with the use of large stokes shift fluorescent dyes
US20220205020A1 (en) 2020-12-30 2022-06-30 Roche Molecular Systems, Inc. Compositions and methods for detection of bacteria and fungi associated with bacterial and candida vaginosis
KR20230142832A (ko) 2021-01-08 2023-10-11 셀라노메, 인크. 생물학적 샘플을 분석하기 위한 디바이스 및 방법
EP4288567A1 (en) 2021-02-05 2023-12-13 F. Hoffmann-La Roche AG Compositions and methods for detection of human parainfluenza viruses 1-4 (hpiv 1-4)
US20220290221A1 (en) 2021-03-15 2022-09-15 Roche Molecular Systems, Inc. Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2) variants having spike protein mutations
CN117858960A (zh) 2021-05-06 2024-04-09 豪夫迈·罗氏有限公司 用于通过双靶标测定来检测丁型肝炎病毒的组合物和方法
WO2023283452A2 (en) 2021-07-09 2023-01-12 Cepheid High-level multiplexing reaction vessel, reagent spotting device and associated methods
US20240329032A1 (en) 2021-07-21 2024-10-03 Aspen Neuroscience, Inc. Methods of differentiating neural cells and predicting engraftment thereof and related compositions
WO2023043280A1 (ko) 2021-09-17 2023-03-23 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
US20230121442A1 (en) 2021-10-06 2023-04-20 Johnson & Johnson Consumer Inc. Method of Quantifying Product Impact on Human Microbiome
CN118076439A (zh) 2021-10-06 2024-05-24 拜耳公司 用于微孔板中的化学、生物化学或分子生物学反应的集成系统
WO2023079032A1 (en) 2021-11-05 2023-05-11 F. Hoffmann-La Roche Ag Compositions and methods for detection of malaria
US20250011882A1 (en) 2021-11-22 2025-01-09 Roche Molecular Systems, Inc. Compositions and Methods for Detecting vanA and/or vanB Genes Associated with Multidrug Resistance
CA3241326A1 (en) 2021-12-03 2023-06-08 Medicinal Genomics Corporation Psilocybe assay
KR20240141247A (ko) 2022-01-26 2024-09-26 주식회사 씨젠 샘플 내 타겟 핵산을 검출하는 방법
WO2023201361A1 (en) 2022-04-15 2023-10-19 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells
US12091715B2 (en) 2022-04-21 2024-09-17 Paragon Genomics, Inc. Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
EP4532695A1 (en) 2022-05-25 2025-04-09 Celgene Corporation Methods of manufacturing t cell therapies
IL317970A (en) 2022-06-29 2025-02-01 Actome Gmbh Identifying biomolecules in single cells
JP2025521678A (ja) 2022-06-30 2025-07-10 カパ バイオシステムズ，インコーポレイティド 古細菌ヒストン様タンパク質を使用したタグメンテーション配列決定ライブラリーインサートサイズの制御
WO2024003260A1 (en) 2022-06-30 2024-01-04 F. Hoffmann-La Roche Ag Compositions and methods for detecting lymphogranuloma venereum (lgv) serovars of chlamydia trachomatis
CN115181787A (zh) * 2022-07-01 2022-10-14 广州医科大学附属第一医院（广州呼吸中心） 一种pcr扩增后的产物快速检测方法
WO2024015766A1 (en) 2022-07-12 2024-01-18 Topogene Inc. Scalable, submicron-resolution replication of dna arrays
WO2024042042A1 (en) 2022-08-24 2024-02-29 F. Hoffmann-La Roche Ag Compositions and methods for detecting monkeypox virus
EP4587594A1 (en) 2022-09-13 2025-07-23 Medicinal Genomics Corporation <smallcapsilocybe assay
IL319782A (en) 2022-09-20 2025-05-01 Cost Bry Pty Ltd Trading As Biomebank Compositions and methods for reducing endogenous sulfide in inflammatory bowel diseases
KR20250110818A (ko) 2022-11-16 2025-07-21 주식회사 씨젠 퀀칭-pto 절단 및 연장(quenching-pto cleavage and extension；q-ptoce) 어세이에 의한 타겟 핵산의 검출
WO2024123131A1 (ko) 2022-12-08 2024-06-13 주식회사 씨젠 체외 진단용 시약 제품의 연구개발 모듈을 업데이트하는 방법 및 연구 개발 관리 장치
WO2024123122A1 (ko) 2022-12-08 2024-06-13 주식회사 씨젠 체외 진단용 시약 제품의 연구개발 관리 장치 및 방법
CN120418449A (zh) 2022-12-25 2025-08-01 豪夫迈·罗氏有限公司 用于检测耐碳青霉烯的醋酸钙不动杆菌-鲍氏不动杆菌(crab)的组合物和方法
WO2024156896A2 (en) 2023-01-26 2024-08-02 Dna Script Enzymatic synthesis of polynucleotide probes
CN120731278A (zh) 2023-02-25 2025-09-30 豪夫迈·罗氏有限公司 用于念珠菌属物种检测的组合物和方法
AU2024228388A1 (en) 2023-02-27 2025-10-02 Adaptive Biotechnologies Corp. Therapeutic t cell receptors targeting kras g12d
CN120917150A (zh) 2023-03-03 2025-11-07 豪夫迈·罗氏有限公司 用于检测b族链球菌（无乳链球菌）和克林霉素抗性基因决定子的组合物和方法
EP4455303A1 (en) * 2023-04-28 2024-10-30 Mobidiag Oy Nucleic acid amplification process controls
WO2024235904A1 (en) 2023-05-16 2024-11-21 Roche Molecular Systems, Inc. Detection of target analytes using multimodal signal probes
WO2025027080A1 (en) 2023-08-03 2025-02-06 F. Hoffmann-La Roche Ag Use of 5'-tailed long primers to improve amplification performance when targeting short primer-binding sites
WO2025072571A1 (en) 2023-09-27 2025-04-03 Cellanome, Inc. Cell culture within microfluidic structures
WO2025202488A1 (en) 2024-03-28 2025-10-02 Roche Diagnostics Gmbh Enhancer of nucleic acid amplification
WO2025245399A1 (en) 2024-05-23 2025-11-27 Indapta Therapeutics, Inc. Method of treating cancer with natural killer cells

Family Cites Families (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1006767A (en) 1910-01-19 1911-10-24 Gen Electric Electric hair-drier.
US1456005A (en) 1922-07-24 1923-05-22 Harris Jack Incubator
US2379474A (en) 1943-11-10 1945-07-03 Bramson Maurice Heating cabinet, incubator, and the like
US3219416A (en) 1962-10-30 1965-11-23 Scientific Industries Apparatus for the automatic chemical sequential treatment and analysis of small quantities of material
US3556659A (en) 1966-02-03 1971-01-19 Applied Physics Corp Laser-excited raman spectrometer
US3616264A (en) 1969-06-30 1971-10-26 Beckman Instruments Inc Temperature-controlled discrete sample analyzer
US3718133A (en) 1971-01-12 1973-02-27 Damon Corp Container unit for liquid samples
US4038055A (en) 1975-10-10 1977-07-26 Block Engineering, Inc. Gas chromatograph for continuous operation with infrared spectrometer
US4168017A (en) 1977-04-05 1979-09-18 Anderwald Cecil E Container means preventing accidental use by children
AU528259B2 (en) 1978-09-14 1983-04-21 Union Carbide Corp Method and apparatus for assaying liquid materials
IT1101295B (it) 1978-11-22 1985-09-28 Erba Strumentazione Camera gas-cromatografica
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
US4420679A (en) 1982-02-26 1983-12-13 Delta Associates, Inc. Gas chromatographic oven using symmetrical flow of preheated - premixed ambient air
US4468423A (en) 1982-11-17 1984-08-28 Arlie Hall Insulating cell element and structures composed thereof
US4468426A (en) * 1982-11-24 1984-08-28 E. I. Du Pont De Nemours And Company Adherable reinforced poly(ethylene terephthalate) composite sheet
US4481405A (en) 1983-04-27 1984-11-06 Malick Franklin S Cooking appliance
US4599169A (en) 1984-02-28 1986-07-08 Varian Associates, Inc. Heating and cooling apparatus for chromatography column
US4701415A (en) 1984-03-02 1987-10-20 Mallinckrodt, Inc. Controlled atmosphere enclosure
JPS60212986A (ja) 1984-04-06 1985-10-25 日本特殊陶業株式会社 直流電圧印加用セラミツクヒ−タ−
JPS60241884A (ja) 1984-05-15 1985-11-30 Tokyo Daigaku 自動サイクリング反応装置およびこれを用いる自動分析装置
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5038852A (en) 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
JPS6212986A (ja) 1985-07-10 1987-01-21 Hitachi Ltd 磁気バブルメモリデバイスのパツケ−ジ
US4740472A (en) 1985-08-05 1988-04-26 The United States Of America As Represented By The United States Department Of Energy Method and apparatus for automated processing and aliquoting of whole blood samples for analysis in a centrifugal fast analyzer
US4868104A (en) * 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US4675300A (en) 1985-09-18 1987-06-23 The Board Of Trustees Of The Leland Stanford Junior University Laser-excitation fluorescence detection electrokinetic separation
CA1273552A (en) 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
EP0232967B1 (en) * 1986-01-10 1993-04-28 Amoco Corporation Competitive homogeneous assay
US4868103A (en) 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
CA1339653C (en) 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4708782A (en) 1986-09-15 1987-11-24 Sepragen Corporation Chromatography column-electrophoresis system
US4684465A (en) 1986-10-10 1987-08-04 Combustion Engineering, Inc. Supercritical fluid chromatograph with pneumatically controlled pump
US4902624A (en) 1987-11-23 1990-02-20 Eastman Kodak Company Temperature cycling cuvette
DE3808942A1 (de) 1988-03-17 1989-09-28 Bio Med Gmbh Ges Fuer Biotechn Inkubator, insbes. fuer die polymerase-ketten-methode
GB8807297D0 (en) 1988-03-26 1988-04-27 Dean P D G Intelligent heating block
US4908112A (en) 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
US4865986A (en) 1988-10-06 1989-09-12 Coy Corporation Temperature control apparatus
US5169511A (en) 1988-11-29 1992-12-08 Isco, Inc. Capillary electrophoresis technique
US5536648A (en) 1988-12-09 1996-07-16 Amrad Corporation Limited Amplified DNA assay using a double stranded DNA binding protein
JPH03505004A (ja) 1989-02-08 1991-10-31 シティ・オブ・ホープ 化学反応処理を連続して行う装置及びその方法
DE69012887D1 (de) 1989-06-20 1994-11-03 Claudio Bonini Prüfröhrchen mit einer linsenförmigen Aussenfläche, insbesondere für automatische klinische Analysen.
US5449621A (en) 1989-07-31 1995-09-12 Biotope, Inc. Method for measuring specific binding assays
US5346672A (en) 1989-11-17 1994-09-13 Gene Tec Corporation Devices for containing biological specimens for thermal processing
US5075556A (en) * 1989-12-01 1991-12-24 Technicon Instruments Corporation Acridine orange derivatives and their use in the quantitation of reticulocytes in whole blood
US5169521A (en) 1989-12-13 1992-12-08 The United States Of America As Represented By The Department Of Health And Human Services Apparatus for countercurrent chromatography separations
US5173163A (en) 1990-01-11 1992-12-22 Isco, Inc. Capillary electrophoresis technique
US5141621A (en) 1990-01-26 1992-08-25 The Board Of Trustees Of The Leland Stanford Junior University Capillary electrophoresis injection device and method
US5207987A (en) * 1990-05-21 1993-05-04 Pb Diagnostic Systems Inc. Temperature controlled chamber for diagnostic analyzer
DE69104775T2 (de) 1990-05-29 1995-06-01 Waters Investments Ltd Verfahren und Vorrichtung zum Durchführen der Kapillarelektroforese.
US7081226B1 (en) 1996-06-04 2006-07-25 University Of Utah Research Foundation System and method for fluorescence monitoring
US5455175A (en) 1990-06-04 1995-10-03 University Of Utah Research Foundation Rapid thermal cycling device
US5187084A (en) 1990-06-22 1993-02-16 The Dow Chemical Company Automatic air temperature cycler and method of use in polymerose chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5269900A (en) 1990-09-13 1993-12-14 University Of North Carolina At Chapel Hill Method and device for high speed separation of complex molecules
DE69128520T2 (de) * 1990-10-31 1998-07-09 Tosoh Corp Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
US5240577A (en) 1990-11-13 1993-08-31 University Of North Carolina At Chapel Hill Two-dimensional high-performance liquid chromatography/capillary electrophoresis
US5131998A (en) 1990-11-13 1992-07-21 The University Of North Carolina At Chapel Hill Two-dimensional high-performance liquid chromatography/capillary electrophoresis
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
US5380489A (en) 1992-02-18 1995-01-10 Eastman Kodak Company Element and method for nucleic acid amplification and detection using adhered probes
EP0533909B1 (en) 1991-03-28 1997-01-08 Perseptive Biosystems, Inc. On-line product identification in a chromatography effluent by subtraction
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
WO1992020778A1 (en) 1991-05-24 1992-11-26 Kindconi Pty Limited Biochemical reaction control
US5340715A (en) 1991-06-07 1994-08-23 Ciba Corning Diagnostics Corp. Multiple surface evanescent wave sensor with a reference
US5316913A (en) 1991-09-06 1994-05-31 Stanford University Neutrophil LECAM-1 as indicator of neutrophil activation
US5114551A (en) 1991-09-30 1992-05-19 Bio-Rad Laboratories, Inc. Multi-point detection method for electrophoresis and chromatography in capillaries
US5116471A (en) 1991-10-04 1992-05-26 Varian Associates, Inc. System and method for improving sample concentration in capillary electrophoresis
ES2159282T3 (es) * 1991-11-07 2001-10-01 Nanotronics Inc Hibridacion de polinucleotidos conjugados con cromoforas y fluoroforos con el fin de generar sistemas de transferencia de energia de donador a donador.
US5348853A (en) 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
DE4234086A1 (de) 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
DE69207580T2 (de) 1992-03-23 1996-08-08 Hoffmann La Roche Verfahren zum DNS-Nachweis
AU4047893A (en) 1992-04-06 1993-11-08 Abbott Laboratories Method and device for detection of nucleic acid or analyte using total internal reflectance
JPH0634546A (ja) 1992-07-17 1994-02-08 Tosoh Corp 蛍光検出器
EP0609431B1 (en) 1992-08-24 1997-10-08 Dade MicroScan Inc. Sealable vessel for containing and processing analytical samples
US5340728A (en) * 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
US5593840A (en) 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
US5364790A (en) 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
US5798215A (en) 1993-02-18 1998-08-25 Biocircuits Corporation Device for use in analyte detection assays
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US6027884A (en) * 1993-06-17 2000-02-22 The Research Foundation Of The State University Of New York Thermodynamics, design, and use of nucleic acid sequences
JP3598123B2 (ja) 1993-07-15 2004-12-08 浜松ホトニクス株式会社 核酸の変性検出装置
ATE208658T1 (de) 1993-07-28 2001-11-15 Pe Corp Ny Vorrichtung und verfahren zur nukleinsäurevervielfältigung
CA2129787A1 (en) * 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
CA2126391C (en) 1993-09-13 2002-01-08 Nobuko Yamamoto Determination of nucleic acid by pcr, measurement of number of microbial cells, genes, or gene-copies by pcr, and measuring-kit employed for the same
US5415839A (en) 1993-10-21 1995-05-16 Abbott Laboratories Apparatus and method for amplifying and detecting target nucleic acids
US5804380A (en) 1993-11-12 1998-09-08 Geron Corporation Telomerase activity assays
US5863726A (en) 1993-11-12 1999-01-26 Geron Corporation Telomerase activity assays
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
DK1921169T3 (da) * 1993-11-12 2012-06-04 Phri Properties Inc Hybridiseringssonder til nukleinsyredetektion, universelle stamceller, fremgangsmåder og kits
GB9401535D0 (en) 1994-01-27 1994-03-23 Steer Rupert P Timing devices
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
JP3383310B2 (ja) 1994-02-01 2003-03-04 ロバート イー． フィールズ， 分子分析器および使用方法
JP3265113B2 (ja) * 1994-03-04 2002-03-11 三菱製紙株式会社 インクジェット記録シート
CA2143365A1 (en) 1994-03-14 1995-09-15 Hugh V. Cottingham Nucleic acid amplification method and apparatus
CA2148140C (en) 1994-04-29 2010-08-10 John W. Sutherland Homogeneous method for assay of double-stranded nucleic acids using fluorescent dyes and kit useful therein
ATE198511T1 (de) 1994-04-29 2001-01-15 Perkin Elmer Corp Verfahren und vorrichtung zur echtzeiterfassung der produkte von nukleinsäureamplifikation
WO1995032306A1 (en) * 1994-05-23 1995-11-30 Biotronics Corporation Method for detecting a target nucleic acid
US5766889A (en) 1994-06-08 1998-06-16 The Perkin-Elmer Corporation Method for determining the characteristics of the concentration growth of target nucleic acid molecules in polymerase chain reaction sample
US5622821A (en) * 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
DE69527125T2 (de) * 1994-11-09 2003-02-27 Hitachi, Ltd. Verfahren und Vorrichtung zur DNS Analyse
JPH11506605A (ja) * 1995-06-07 1999-06-15 アボツト・ラボラトリーズ 増幅反応におけるバックグラウンドを減少させるためにプローブをマスキングする方法
US6008373A (en) * 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
WO1996041864A1 (en) 1995-06-13 1996-12-27 The Regents Of The University Of California Diode laser heated micro-reaction chamber with sample detection means
US5863727A (en) * 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
ATE318327T1 (de) * 1996-06-04 2006-03-15 Univ Utah Res Found Fluoreszenz-donor-akzeptor paar
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
EP0822261B1 (en) * 1996-07-29 2002-07-03 Academia Sinica Methods for rapid antimicrobial susceptibility testing
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6153411A (en) * 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification

Also Published As

Publication number Publication date
CA2658290C (en) 2012-04-10
JP4118932B2 (ja) 2008-07-16
JP4540754B2 (ja) 2010-09-08
CA2591550A1 (en) 1997-12-11
EP1033411A3 (en) 2000-10-11
CA2658290A1 (en) 1997-12-11
JP4401416B2 (ja) 2010-01-20
JP2010046063A (ja) 2010-03-04
CA2257109A1 (en) 1997-12-11
EP0912766A1 (en) 1999-05-06
EP0912766B2 (en) 2011-12-14
AU3481297A (en) 1998-01-05
ES2326050T3 (es) 2009-09-29
US20020058258A1 (en) 2002-05-16
ES2243393T3 (es) 2005-12-01
EP1033411B1 (en) 2006-02-22
US20090311673A1 (en) 2009-12-17
JP2000509608A (ja) 2000-08-02
US20040002098A1 (en) 2004-01-01
ATE295427T1 (de) 2005-05-15
NZ333136A (en) 2000-03-27
CA2257109C (en) 2009-10-06
DK0912766T4 (da) 2012-04-02
DE69733282T2 (de) 2006-01-19
EP1179600A1 (en) 2002-02-13
WO1997046714A1 (en) 1997-12-11
US8343754B2 (en) 2013-01-01
US7160998B2 (en) 2007-01-09
NZ502323A (en) 2001-09-28
EP1179600B1 (en) 2005-05-11
US6232079B1 (en) 2001-05-15
ATE428801T1 (de) 2009-05-15
DK0912766T3 (da) 2009-08-10
ES2326050T5 (es) 2012-04-26
PT1179600E (pt) 2005-08-31
DE69735313T2 (de) 2006-11-02
DE69739357D1 (de) 2009-05-28
PT912766E (pt) 2009-07-16
US6245514B1 (en) 2001-06-12
JP2007185188A (ja) 2007-07-26
JP2008173127A (ja) 2008-07-31
US20060029965A1 (en) 2006-02-09
DK1179600T3 (da) 2005-09-05
US7670832B2 (en) 2010-03-02
US6174670B1 (en) 2001-01-16
EP1033411A2 (en) 2000-09-06
US20090258414A1 (en) 2009-10-15
US6569627B2 (en) 2003-05-27
US20120258524A1 (en) 2012-10-11
DE69735313D1 (de) 2006-04-27
ATE318327T1 (de) 2006-03-15
EP0912766B1 (en) 2009-04-15
DE69733282D1 (de) 2005-06-16
AU726501B2 (en) 2000-11-09

Similar Documents

Publication Publication Date Title
CA2591550C (en) Monitoring hybridization during pcr
EP1117834B1 (en) Multiplex genotyping using fluorescent hybridization probes
EP0912760B1 (en) System and methods for monitoring for dna amplification by fluorescence
CA2525482C (en) Improved system for multicolor real-time pcr comprising 3 to 6 pairs of forester resonance energy transfer (fret) probes
JP2005504543A (ja) 定量的ｐｃｒのための適応ベースラインアルゴリズム
Wittwer et al. Fluorescence monitoring of rapid cycle PCR for quantification
US20060040303A1 (en) Reaction mixture for positioning a reaction vessel relative to a detection unit
KR100479789B1 (ko) Pcr하는동안에하이브리드반응의모니터방법
AU747662B2 (en) Fluorescent donor-acceptor pair
KR20000016161A (ko) 형광에 의한 dna 증폭 모니터링 방법 및 시스템

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20170605