IN2015DN01609A - - Google Patents

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Publication number
IN2015DN01609A
IN2015DN01609A IN1609DEN2015A IN2015DN01609A IN 2015DN01609 A IN2015DN01609 A IN 2015DN01609A IN 1609DEN2015 A IN1609DEN2015 A IN 1609DEN2015A IN 2015DN01609 A IN2015DN01609 A IN 2015DN01609A
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IN
India
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presented
methods
amplicons
approaches
quantifying
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English (en)
Inventor
Imran R Malik
Xiomara L Madero
Erika F Garcia
Sheel M Shah
Axel Scherer
Original Assignee
California Inst Of Techn
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Publication of IN2015DN01609A publication Critical patent/IN2015DN01609A/en

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6486Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Algebra (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mathematical Analysis (AREA)
  • Mathematical Optimization (AREA)
  • Mathematical Physics (AREA)
  • Pure & Applied Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Time-Division Multiplex Systems (AREA)
IN1609DEN2015 2012-08-03 2013-08-02 IN2015DN01609A (enExample)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261679547P 2012-08-03 2012-08-03
PCT/US2013/053512 WO2014022827A1 (en) 2012-08-03 2013-08-02 Multiplexing and quantification in pcr with reduced hardware and requirements

Publications (1)

Publication Number Publication Date
IN2015DN01609A true IN2015DN01609A (enExample) 2015-07-03

Family

ID=50025861

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1609DEN2015 IN2015DN01609A (enExample) 2012-08-03 2013-08-02

Country Status (5)

Country Link
US (3) US9791372B2 (enExample)
EP (2) EP2880184B1 (enExample)
CN (2) CN104662172B (enExample)
IN (1) IN2015DN01609A (enExample)
WO (1) WO2014022827A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104271767B (zh) 2012-02-03 2017-08-25 加州理工学院 多路生化测定中信号的编码和解码
IN2015DN01609A (enExample) 2012-08-03 2015-07-03 California Inst Of Techn
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
DE102014105129B3 (de) * 2014-04-10 2015-07-02 Kist Europe-Korea Institute of Science and Technologie Europe Forschungsgesellschaft mbh Verfahren und Master-Mix für die quantitative Echtzeit-PCR für Multiplex-Ziel-Nukleinsäuremoleküle
CN103884679B (zh) * 2014-04-18 2016-01-20 山西大学 结合卡尔曼滤波器的腔衰荡光谱技术气体浓度监测方法
US10876155B2 (en) 2014-12-09 2020-12-29 Seegene, Inc. Differentiation of signals for target nucleic acid sequences
EP3130679B1 (de) * 2015-08-13 2018-02-28 Cladiac GmbH Verfahren und testsystem zum nachweis und/oder quantifizieren einer ziel-nukleinsäure in einer probe
CN109642252A (zh) * 2016-06-17 2019-04-16 加州理工学院 核酸反应及相关方法和组合物
EP3559261A4 (en) * 2016-12-23 2020-12-23 The Regents of The University of California HIGH RESOLUTION DIGITAL FUSION PROCESS AND DEVICE
US20190112636A1 (en) * 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
WO2019204357A1 (en) 2018-04-17 2019-10-24 ChromaCode, Inc. Methods and systems for multiplex analysis
CN108841939B (zh) * 2018-06-21 2020-09-22 北京致雨生物科技有限公司 多重数字pcr浓度测量方法及微滴式数字pcr系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
JP7221491B2 (ja) * 2018-09-18 2023-02-14 株式会社ミズホメディー 複数の標的核酸を検出するキットを用いる検出方法
CA3147297A1 (en) 2019-07-16 2021-01-21 Meliolabs Inc. Methods and devices for single-cell based digital high resolution melt
WO2025235889A1 (en) * 2024-05-10 2025-11-13 Guardant Health, Inc. Methods involving multiplexed pooled pcr

Family Cites Families (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3785591T2 (de) 1986-01-10 1993-09-02 Amoco Corp Kompetitiver homogener test.
US6127155A (en) 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US5079351A (en) 1986-11-26 1992-01-07 Cetus Corporation Oligonucleotides and kits for detection of htlvi and htlvii viruses by hybridization
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP0519338B1 (en) 1991-06-20 1996-08-28 F. Hoffmann-La Roche Ag Improved methods for nucleic acid amplification
DE69333650T2 (de) 1992-02-19 2006-01-12 The Public Health Research Institute Of The City Of New York, Inc. Neue anordnungn von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzieren und manipulieren von nukleinsäuren
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5948360A (en) 1994-07-11 1999-09-07 Tekmar Company Autosampler with robot arm
EP1522845A1 (en) 1995-02-02 2005-04-13 Chugai Seiyaku Kabushiki Kaisha Method for assay of analyte by adjustment of chemiluminescence
WO1996036436A1 (en) 1995-04-25 1996-11-21 Irori Remotely programmable matrices with memories and uses thereof
JP4040087B2 (ja) 1995-05-05 2008-01-30 アプレラ コーポレイション Pcr増幅とハイブリダイゼーションプロービングアッセイとを組み合わせた方法およびそのための試薬
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US20050112637A1 (en) 1995-09-08 2005-05-26 Chatterjee Deb K. Mutant DNA polymerases and uses thereof
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6007994A (en) 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization
WO1997034015A1 (en) 1996-03-15 1997-09-18 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
PT938320E (pt) 1996-03-26 2010-09-22 Michael S Kopreski Método que permite a utilização de arn extracelular extraído de plasma ou de soro para detectar, monitorizar ou avaliar o cancro
WO1997046714A1 (en) 1996-06-04 1997-12-11 University Of Utah Research Foundation Monitoring hybridization during pcr
US5834203A (en) * 1997-08-25 1998-11-10 Applied Spectral Imaging Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore
IL135593A (en) 1997-10-14 2004-06-01 Luminex Corp Precisely fluorescently painted polymeric microspheres and methods for making and using them
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
WO1999052708A1 (en) 1998-04-13 1999-10-21 Luminex Corporation Liquid labeling with fluorescent microparticles
EP2360271A1 (en) 1998-06-24 2011-08-24 Illumina, Inc. Decoding of array sensors with microspheres
JP4646404B2 (ja) 1998-07-02 2011-03-09 ジェン−プローブ・インコーポレーテッド 分子トーチ(torch)
US6642062B2 (en) 1998-09-03 2003-11-04 Trellis Bioinformatics, Inc. Multihued labels
WO2000065094A2 (en) 1999-04-22 2000-11-02 The Albert Einstein College Of Medicine Of Yeshiva University Assay of gene expression patterns by multi-fluor fish
WO2001007640A2 (en) 1999-07-23 2001-02-01 The Secretary Of State For The Home Department Method for detecting single nucleotide polymorphisms
WO2001031062A1 (en) 1999-10-22 2001-05-03 The Public Health Research Institute Of The City Of New York, Inc. Assays for short sequence variants
AU2001234883A1 (en) * 2000-02-10 2001-08-20 The Penn State Research Foundation Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion
US20040053230A1 (en) * 2001-03-07 2004-03-18 Schaffer Michael Edward Real time quantitative pcr with intercalating dye for single and multiplex target dna
EP1715063B1 (en) 2000-03-29 2011-01-26 LGC Limited Hybridisation beacon and method of rapid sequence detection and discrimination
US6734420B2 (en) 2000-04-06 2004-05-11 Quantum Dot Corporation Differentiable spectral bar code methods and systems
CA2314398A1 (en) 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US20020142345A1 (en) 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
EP2089413A4 (en) 2001-02-21 2009-10-28 Amnis Corp METHOD AND DEVICE FOR MARKING AND ANALYZING CELLULAR CONSTITUENTS
EP1373569A4 (en) 2001-03-02 2006-02-08 Univ Pittsburgh PCR METHOD
WO2003002979A2 (en) 2001-06-28 2003-01-09 Illumina, Inc. Multiplex decoding of array sensors with microspheres
CA2450725A1 (en) 2001-06-28 2003-01-09 Advanced Research And Technology Institute, Inc. Methods of preparing multicolor quantum dot tagged beads and conjugates thereof
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
EP1288308A1 (en) 2001-08-28 2003-03-05 Roche Diagnostics GmbH A method for the determination of multiple analytes
US6852491B2 (en) 2001-09-04 2005-02-08 Abbott Laboratories Amplification and detection reagents for HIV-1
US20030134320A1 (en) 2002-01-15 2003-07-17 Myriad Genetics, Incorporated Method system and computer program product for quality assurance in detecting biochemical markers
EP1426448A1 (en) 2002-12-03 2004-06-09 bioMérieux BV Method for lowering the effects of sequence variations in a diagnostic hybridization assay, probe for use in the assay and assay
EP2284278A1 (en) 2003-04-04 2011-02-16 Roche Diagnostics GmbH Improved system for multi color real time PCR
US7385043B1 (en) 2003-04-30 2008-06-10 The Public Health Research Institute Of The City Of New York, Inc. Homogeneous multiplex screening assays and kits
US20050053950A1 (en) 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
TWI232934B (en) 2003-11-19 2005-05-21 Ind Tech Res Inst A biochip containing splitable reaction confinement and method for producing same and application thereof
WO2005051967A2 (en) 2003-11-19 2005-06-09 Allelogic Biosciences Corp. Oligonucleotides labeled with a plurality of fluorophores
EP1727913B8 (en) 2004-03-24 2009-08-19 Applied Biosystems, LLC Ligation and amplification reactions for determining target molecules
JP4972541B2 (ja) 2004-04-01 2012-07-11 バイオ−ラッド ラボラトリーズ インコーポレーティッド 標識されたプローブおよび3’→5’エキソヌクレアーゼ活性を用いた定量的増幅方法
EP1745155B1 (en) 2004-05-07 2014-10-15 Cepheid Multiplexed detection of biological agents
US7575864B2 (en) 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA
DE102004043870B4 (de) 2004-09-10 2009-11-19 Eppendorf Ag Verfahren zur Erweiterung des dynamischen Erfassungsbereich bei Mikroarrays
AU2005287517A1 (en) 2004-09-24 2006-03-30 Universitaet Bern Molecular beacons
KR101424812B1 (ko) * 2004-10-18 2014-08-07 브랜데이스 유니버시티 핵산 증폭을 위한 프라이머, 프로브 및 방법
JP4999103B2 (ja) 2004-10-27 2012-08-15 セフィード 閉鎖系の多段階核酸増幅反応
JP2008533974A (ja) 2005-01-21 2008-08-28 サード・ウェーブ・テクノロジーズ・インク 核酸分子の増大したダイナミックレンジ検出のための組成物および方法
US7507575B2 (en) * 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
US7709249B2 (en) * 2005-04-01 2010-05-04 3M Innovative Properties Company Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector
US7919244B2 (en) 2005-06-16 2011-04-05 Biotools Biotechnological & Medical Laboratories, S.A. Nucleic acid detection method involving the direct generation of a measurable signal
US7465561B2 (en) 2005-06-30 2008-12-16 Roche Molecular Systems, Inc. Probes and methods for hepatitis C virus typing using single probe analysis
US7553955B2 (en) 2005-09-01 2009-06-30 The Trustees Of The University Of Pennsylvania Molecular beacons, methods and kits for detecting DNA damage response
EP1937834B1 (en) 2005-09-01 2014-06-11 AusDiagnostics Pty Ltd. Methods for the amplification, quantitation and identification of nucleic acids
CN1936019B (zh) 2005-10-18 2013-01-30 厦门大学 用于多重实时核酸扩增检测的探针编码方法
CA2635215C (en) 2005-12-23 2016-08-30 Nanostring Technologies, Inc. Compositions comprising oriented, immobilized macromolecules and methods for their preparation
WO2007076129A2 (en) 2005-12-23 2007-07-05 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
WO2007080583A2 (en) 2006-01-10 2007-07-19 Applied Spectral Imaging Ltd. Methods and systems for analyzing biological samples
US8455184B2 (en) 2006-04-18 2013-06-04 The United States Of America As Represented By The Secretary Of The Air Force Differential multiplexing with pattern recognition
US20090062129A1 (en) 2006-04-19 2009-03-05 Agencourt Personal Genomics, Inc. Reagents, methods, and libraries for gel-free bead-based sequencing
EP2030011B1 (en) 2006-05-22 2017-02-01 Nanostring Technologies, Inc. Systems and methods for analyzing nanoreporters
EP2210955A3 (en) 2006-05-23 2010-09-15 Molecular Detection, Inc. Ambient temperature stable kits for molecular diagnostics
EP2041318A2 (en) 2006-06-26 2009-04-01 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
WO2008036614A1 (en) 2006-09-18 2008-03-27 California Institute Of Technology Apparatus for detecting target molecules and related methods
EP2407558A1 (en) 2006-10-31 2012-01-18 Noxxon Pharma AG Methods for the detection of a single- or double-stranded nucleic acid molecule
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
US7930106B2 (en) 2006-11-30 2011-04-19 Gen-Probe Incorporated Quantitative method employing adjustment of pre-defined master calibration curves
KR101433208B1 (ko) * 2006-12-13 2014-08-22 루미넥스 코포레이션 실시간으로 pcr의 다중 분석을 위한 시스템과 방법
CA2580589C (en) 2006-12-19 2016-08-09 Fio Corporation Microfluidic detection system
US20090042735A1 (en) 2007-03-05 2009-02-12 Blair Steven M Methods and Compositions Related to Nucleic Acid Detection
WO2008118998A2 (en) 2007-03-27 2008-10-02 Primera Biosystems Inc. Method for multiplex detection and quantitation of nucleic acids
AU2008237018B2 (en) 2007-04-10 2014-04-03 Bruker Spatial Biology, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
WO2009003493A2 (en) 2007-07-03 2009-01-08 Dako Denmark A/S Mhc multimers, methods for their generation, labeling and use
US8374802B2 (en) * 2007-08-29 2013-02-12 Eppendorf Ag Device and method for radiometric measurement of a plurality of samples
EP2195453A4 (en) 2007-09-20 2011-02-02 Molecular Plant Breeding Nominees Ltd METHOD FOR AMPLIFYING NUCLEIC ACID
KR20090127893A (ko) 2007-12-26 2009-12-14 아크레이 가부시키가이샤 표적 핵산 서열의 증폭 방법 및 그것에 이용하는 프로브
EP2116614A1 (en) 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
GB0812862D0 (en) 2008-07-14 2008-08-20 Lumora Ltd Nucleic acid amplification
CN102171366B (zh) 2008-07-31 2014-11-26 奥西泰克有限公司 多重扩增与检测
EP2315844A1 (en) 2008-08-08 2011-05-04 Smiths Detection Inc. Detection algorithm for pcr assay
WO2010019826A1 (en) 2008-08-14 2010-02-18 Nanostring Technologies, Inc Stable nanoreporters
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
BRPI0916143A2 (pt) * 2008-11-21 2015-11-03 Koninkl Philips Electronics Nv "método para monitorar a multiplicação de um ou mais ácidos nucleicos visados"
WO2010063732A1 (en) 2008-12-01 2010-06-10 Biotype Ag Novel combination of fluorescent dyes for the detection of nucleic acids
US9534255B2 (en) 2008-12-17 2017-01-03 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
US9689028B2 (en) * 2008-12-22 2017-06-27 University Of Utah Foundation Monochrome multiplex quantitative PCR
US8361738B2 (en) 2009-03-06 2013-01-29 California Institute Of Technology Methods for quantitative target detection and related devices and systems
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
CN101570782B (zh) 2009-03-20 2012-01-11 曹际娟 乳制品中8种致病菌检测试剂盒及其检测方法
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
US20120040349A1 (en) 2009-05-12 2012-02-16 Piia Von Lode Method for detecting nucleic acids
US9359639B2 (en) 2009-05-26 2016-06-07 Xiamen University Method for the detection of multiple single nucleotide variations or single nucleotide polymorphisms in a single tube
US8691504B2 (en) 2009-05-26 2014-04-08 Xiamen University Method for detecting variations in nucleic acid sequences
EP2280080A1 (en) 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides
MX352971B (es) 2009-09-10 2017-12-15 Diasorin S P A Compensacion para diafonia espectral en amplificacion de acido nucleico multiplexado.
KR101590175B1 (ko) 2009-09-24 2016-01-29 주식회사 씨젠 반복적 엑소핵산 절단 반응에 의한 타겟 핵산서열의 검출
US8962251B2 (en) 2009-10-08 2015-02-24 Medical Service Consultation International, Llc Methods and compositions for identifying sulfur and iron modifying bacteria
ES2627063T3 (es) 2009-10-13 2017-07-26 Nanostring Technologies, Inc Detección de proteína a través de nanoinformadores
US9752187B2 (en) 2009-12-11 2017-09-05 Nucleix Categorization of DNA samples
AU2011215753B2 (en) 2010-02-11 2015-09-03 Bruker Spatial Biology, Inc. Compositions and methods for the detection of small RNAs
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP3392349A1 (en) 2010-02-12 2018-10-24 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US20110229888A1 (en) 2010-03-16 2011-09-22 Hengen Paul N Compositions and Methods for the Detection of Genomic Features
WO2011146629A2 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
CN102269759B (zh) * 2010-06-07 2014-05-07 常州楚天生物科技有限公司 一种基于免疫pcr和dna熔解曲线分析的多重检测方法
CN102985566B (zh) 2010-06-30 2015-06-17 简.探针公司 使用分析物和过程控制信号的单次读取确定而鉴定含分析物的样品的方法和设备
US8877464B2 (en) 2010-07-29 2014-11-04 Roche Molecular Systems, Inc. Qualitative and quantitative detection of microbial nucleic acids
GB201017978D0 (en) * 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
EP2665995B1 (en) 2011-01-20 2018-08-29 University of Washington through its Center for Commercialization Methods and systems for performing digital measurements
WO2012106428A2 (en) 2011-02-03 2012-08-09 University Of Florida Research Foundation, Inc. Detection methods for target dna
WO2012129115A2 (en) 2011-03-18 2012-09-27 University Of South Florida Materials and methods for profiling micrornas
JP2014509865A (ja) 2011-03-18 2014-04-24 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド シグナルの組合せ使用による多重化デジタルアッセイ
US9758834B2 (en) 2011-03-28 2017-09-12 Nanostring Technologies, Inc. Compositions and methods for diagnosing cancer
AU2012272763B2 (en) 2011-06-24 2016-11-03 Nanostring Technologies, Inc. Multivariate diagnostic assays and methods for using same
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US8858623B2 (en) 2011-11-04 2014-10-14 Valtech Cardio, Ltd. Implant having multiple rotational assemblies
CN102559868B (zh) * 2011-11-28 2014-11-05 厦门大学 一种单管定性并定量检测多个目标核酸序列的方法
ES2952728T3 (es) 2011-12-22 2023-11-03 Harvard College Métodos para la detección de analitos
CN104271767B (zh) 2012-02-03 2017-08-25 加州理工学院 多路生化测定中信号的编码和解码
CA2877933A1 (en) 2012-06-27 2014-01-03 Yuri Bushkin Rapid assays for t-cell activation by rna measurements using flow cytometry
IN2015DN01609A (enExample) 2012-08-03 2015-07-03 California Inst Of Techn
GB201218909D0 (en) 2012-10-22 2012-12-05 Univ Singapore Assay for the parallel detection of biological material based on PCR
EP2948566B1 (en) 2013-01-24 2025-03-26 California Institute of Technology Chromophore-based characterization and detection methods
EP2971095B1 (en) 2013-03-12 2019-11-20 Life Technologies Corporation Universal reporter-based genotyping methods, reaction mixture and kit
EP2873740A1 (en) 2013-11-19 2015-05-20 Oncodiag Methods for the surveillance, diagnosis and screening of bladder cancer
US9670530B2 (en) 2014-01-30 2017-06-06 Illumina, Inc. Haplotype resolved genome sequencing
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
CA2985134A1 (en) 2014-05-09 2015-11-12 Sebastian Gromminger Detection of dna that originates from a specific cell-type and related methods
ES2835101T3 (es) 2016-02-10 2021-06-21 Univ Michigan Regents Detección de ácidos nucleicos
EP4219519A3 (en) 2016-04-01 2023-09-13 Chromacode, Inc. Competitive probes for engineering signal generation
CN109642252A (zh) 2016-06-17 2019-04-16 加州理工学院 核酸反应及相关方法和组合物
US11435338B2 (en) 2016-10-24 2022-09-06 Ontera Inc. Fractional abundance of polynucleotide sequences in a sample
CN118668012A (zh) 2017-06-28 2024-09-20 克罗玛科德公司 使用微滴式pcr系统的多重荧光测量
US20190112636A1 (en) 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
US20200407794A1 (en) 2018-02-28 2020-12-31 ChromaCode, Inc. Molecular targets for fetal nucleic acid analysis

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US20180052110A1 (en) 2018-02-22
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EP2880184A4 (en) 2016-03-30
US11959856B2 (en) 2024-04-16
US20240201090A1 (en) 2024-06-20
CN108753932B (zh) 2022-12-02
EP2880184B1 (en) 2021-03-31
CN104662172A (zh) 2015-05-27
US20140038195A1 (en) 2014-02-06
WO2014022827A1 (en) 2014-02-06
US9791372B2 (en) 2017-10-17
CN108753932A (zh) 2018-11-06
EP3901243A1 (en) 2021-10-27

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