IN2015DN01609A - - Google Patents

Download PDF

Info

Publication number
IN2015DN01609A
IN2015DN01609A IN1609DEN2015A IN2015DN01609A IN 2015DN01609 A IN2015DN01609 A IN 2015DN01609A IN 1609DEN2015 A IN1609DEN2015 A IN 1609DEN2015A IN 2015DN01609 A IN2015DN01609 A IN 2015DN01609A
Authority
IN
India
Prior art keywords
presented
methods
amplicons
approaches
quantifying
Prior art date
Application number
Other languages
English (en)
Inventor
Imran R Malik
Xiomara L Madero
Erika F Garcia
Sheel M Shah
Axel Scherer
Original Assignee
California Inst Of Techn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Inst Of Techn filed Critical California Inst Of Techn
Publication of IN2015DN01609A publication Critical patent/IN2015DN01609A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Algebra (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mathematical Analysis (AREA)
  • Mathematical Optimization (AREA)
  • Mathematical Physics (AREA)
  • Pure & Applied Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Time-Division Multiplex Systems (AREA)
IN1609DEN2015 2012-08-03 2013-08-02 IN2015DN01609A (enExample)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261679547P 2012-08-03 2012-08-03
PCT/US2013/053512 WO2014022827A1 (en) 2012-08-03 2013-08-02 Multiplexing and quantification in pcr with reduced hardware and requirements

Publications (1)

Publication Number Publication Date
IN2015DN01609A true IN2015DN01609A (enExample) 2015-07-03

Family

ID=50025861

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1609DEN2015 IN2015DN01609A (enExample) 2012-08-03 2013-08-02

Country Status (5)

Country Link
US (3) US9791372B2 (enExample)
EP (2) EP3901243A1 (enExample)
CN (2) CN108753932B (enExample)
IN (1) IN2015DN01609A (enExample)
WO (1) WO2014022827A1 (enExample)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107326085B (zh) 2012-02-03 2022-05-03 加州理工学院 多路生化测定中信号的编码和解码
IN2015DN01609A (enExample) * 2012-08-03 2015-07-03 California Inst Of Techn
WO2015147370A1 (en) * 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
DE102014105129B3 (de) * 2014-04-10 2015-07-02 Kist Europe-Korea Institute of Science and Technologie Europe Forschungsgesellschaft mbh Verfahren und Master-Mix für die quantitative Echtzeit-PCR für Multiplex-Ziel-Nukleinsäuremoleküle
CN103884679B (zh) * 2014-04-18 2016-01-20 山西大学 结合卡尔曼滤波器的腔衰荡光谱技术气体浓度监测方法
KR102084405B1 (ko) 2014-12-09 2020-03-04 주식회사 씨젠 타겟 핵산 서열에 대한 시그널의 구별
EP3130679B1 (de) * 2015-08-13 2018-02-28 Cladiac GmbH Verfahren und testsystem zum nachweis und/oder quantifizieren einer ziel-nukleinsäure in einer probe
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
EP3559261A4 (en) * 2016-12-23 2020-12-23 The Regents of The University of California HIGH RESOLUTION DIGITAL FUSION PROCESS AND DEVICE
US20190112636A1 (en) * 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
CN108841939B (zh) * 2018-06-21 2020-09-22 北京致雨生物科技有限公司 多重数字pcr浓度测量方法及微滴式数字pcr系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
JP7221491B2 (ja) * 2018-09-18 2023-02-14 株式会社ミズホメディー 複数の標的核酸を検出するキットを用いる検出方法
KR102789582B1 (ko) 2019-07-16 2025-03-31 멜리오랩스 인코포레이티드 단일-세포 기반 디지털 고해상도 용융을 위한 방법 및 장치
WO2025235889A1 (en) * 2024-05-10 2025-11-13 Guardant Health, Inc. Methods involving multiplexed pooled pcr

Family Cites Families (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0232967B1 (en) 1986-01-10 1993-04-28 Amoco Corporation Competitive homogeneous assay
US6127155A (en) 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US5079351A (en) 1986-11-26 1992-01-07 Cetus Corporation Oligonucleotides and kits for detection of htlvi and htlvii viruses by hybridization
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
ES2091976T3 (es) 1991-06-20 1996-11-16 Hoffmann La Roche Metodos perfeccionados para la amplificacion del acido nucleico.
AU3728093A (en) 1992-02-19 1993-09-13 Public Health Research Institute Of The City Of New York, Inc., The Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5948360A (en) 1994-07-11 1999-09-07 Tekmar Company Autosampler with robot arm
AU709045B2 (en) 1995-02-02 1999-08-19 Chugai Seiyaku Kabushiki Kaisha Method for assay of analyte by adjustment of chemiluminescence
KR19990008052A (ko) 1995-04-25 1999-01-25 마이클 피 노바 기억 장치를 갖는 원격적으로 프로그램 가능한 매트릭스 및 이의 용도
EP0826066B1 (en) 1995-05-05 2000-09-13 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US20050112637A1 (en) 1995-09-08 2005-05-26 Chatterjee Deb K. Mutant DNA polymerases and uses thereof
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6007994A (en) 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization
US6156504A (en) 1996-03-15 2000-12-05 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
ES2347854T5 (es) 1996-03-26 2014-07-24 Michael S. Kopreski Método que permite el uso de RNA extracelular extraído de plasma o suero para detectar, monitorizar o evaluar un cáncer
ATE428801T1 (de) 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5834203A (en) * 1997-08-25 1998-11-10 Applied Spectral Imaging Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore
JP4357112B2 (ja) 1997-10-14 2009-11-04 ルミネックス コーポレイション 精密蛍光染色された粒子及びその製造及び使用方法
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
AU3555599A (en) 1998-04-13 1999-11-01 Luminex Corporation Liquid labeling with fluorescent microparticles
US7060431B2 (en) 1998-06-24 2006-06-13 Illumina, Inc. Method of making and decoding of array sensors with microspheres
JP4646404B2 (ja) 1998-07-02 2011-03-09 ジェン−プローブ・インコーポレーテッド 分子トーチ（ｔｏｒｃｈ）
US6642062B2 (en) 1998-09-03 2003-11-04 Trellis Bioinformatics, Inc. Multihued labels
EP1171636A2 (en) 1999-04-22 2002-01-16 Albert Einstein College Of Medicine Of Yeshiva University Assay of gene expression patterns by multi-fluor fish
AU782170B2 (en) 1999-07-23 2005-07-07 Forensic Science Service Ltd. Improvements in and relating to analysis of DNA
DE60030145T2 (de) 1999-10-22 2008-01-10 PHRI Properties, Inc., Newark Nachweisverfahren für kurze sequenzvarianten
AU2001234883A1 (en) * 2000-02-10 2001-08-20 The Penn State Research Foundation Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion
US20040053230A1 (en) 2001-03-07 2004-03-18 Schaffer Michael Edward Real time quantitative pcr with intercalating dye for single and multiplex target dna
DK1715063T3 (da) 2000-03-29 2011-05-16 Lgc Ltd Hydridiseringsbeacon og fremgangsmåde til hurtig sekvensdetektion og distinktion
US6734420B2 (en) 2000-04-06 2004-05-11 Quantum Dot Corporation Differentiable spectral bar code methods and systems
CA2314398A1 (en) 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US20020142345A1 (en) 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
AU2002324422A1 (en) 2001-02-21 2002-12-23 Amnis Corporation Method and apparatus for labeling and analyzing cellular components
WO2002070751A1 (en) 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
EP1410031A2 (en) 2001-06-28 2004-04-21 ADVANCED RESEARCH AND TECHNOLOGY INSTITUTE, Inc. Methods of preparing multicolor quantum dot tagged beads and conjugates thereof
AU2002322457A1 (en) 2001-06-28 2003-03-03 Illumina, Inc. Multiplex decoding of array sensors with microspheres
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
EP1288308A1 (en) 2001-08-28 2003-03-05 Roche Diagnostics GmbH A method for the determination of multiple analytes
US6852491B2 (en) 2001-09-04 2005-02-08 Abbott Laboratories Amplification and detection reagents for HIV-1
US20030134320A1 (en) 2002-01-15 2003-07-17 Myriad Genetics, Incorporated Method system and computer program product for quality assurance in detecting biochemical markers
EP1426448A1 (en) 2002-12-03 2004-06-09 bioMérieux BV Method for lowering the effects of sequence variations in a diagnostic hybridization assay, probe for use in the assay and assay
WO2004087950A2 (en) 2003-04-04 2004-10-14 Roche Diagnostics Gmbh Improved system for multi color real time pcr
US7385043B1 (en) 2003-04-30 2008-06-10 The Public Health Research Institute Of The City Of New York, Inc. Homogeneous multiplex screening assays and kits
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
TWI232934B (en) 2003-11-19 2005-05-21 Ind Tech Res Inst A biochip containing splitable reaction confinement and method for producing same and application thereof
WO2005051967A2 (en) 2003-11-19 2005-06-09 Allelogic Biosciences Corp. Oligonucleotides labeled with a plurality of fluorophores
US7604937B2 (en) 2004-03-24 2009-10-20 Applied Biosystems, Llc Encoding and decoding reactions for determining target polynucleotides
US7445898B2 (en) 2004-04-01 2008-11-04 Bio-Rad Laboratories, Inc. Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity
EP1745155B1 (en) 2004-05-07 2014-10-15 Cepheid Multiplexed detection of biological agents
US7575864B2 (en) 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA
DE102004043870B4 (de) 2004-09-10 2009-11-19 Eppendorf Ag Verfahren zur Erweiterung des dynamischen Erfassungsbereich bei Mikroarrays
US7671184B2 (en) 2004-09-24 2010-03-02 Universitact Bern Molecular beacons
ES2668467T3 (es) * 2004-10-18 2018-05-18 Brandeis University Métodos de amplificación LATE y secuenciación de un ácido nucleico
CN101068932B (zh) 2004-10-27 2013-02-13 塞弗德公司 封闭系统多阶段核酸扩增反应
EP1838880A2 (en) 2005-01-21 2007-10-03 Third Wave Technologies, Inc. Methods and compositions for increased dynamic range detection of nucleic acid molecules
US7709249B2 (en) * 2005-04-01 2010-05-04 3M Innovative Properties Company Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector
US7507575B2 (en) * 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
AU2005333512A1 (en) 2005-06-16 2006-12-28 Biotools Biotechnological & Medical Laboratories, S.A. Nucleic acid detection method involving the direct generation of a measurable signal
US7919242B2 (en) 2005-06-30 2011-04-05 Roche Molecular Systems, Inc. Light emission modifiers and their uses in nucleic acid detection, amplification and analysis
US7553955B2 (en) 2005-09-01 2009-06-30 The Trustees Of The University Of Pennsylvania Molecular beacons, methods and kits for detecting DNA damage response
ES2494922T3 (es) 2005-09-01 2014-09-16 Ausdiagnostics Pty Ltd. Métodos para la amplificación, cuantificación e identificación de ácidos nucleicos
CN1936019B (zh) 2005-10-18 2013-01-30 厦门大学 用于多重实时核酸扩增检测的探针编码方法
JP5700911B2 (ja) 2005-12-23 2015-04-15 ナノストリング テクノロジーズ，インコーポレーテッド 配向され、固定化された巨大分子を含む組成物とその製造法
ATE525482T1 (de) 2005-12-23 2011-10-15 Nanostring Technologies Inc Nanoreporter und verfahren zu deren herstellung und verwendung
US9133506B2 (en) 2006-01-10 2015-09-15 Applied Spectral Imaging Ltd. Methods and systems for analyzing biological samples
US8455184B2 (en) 2006-04-18 2013-06-04 The United States Of America As Represented By The Secretary Of The Air Force Differential multiplexing with pattern recognition
JP2009538123A (ja) 2006-04-19 2009-11-05 アプライド バイオシステムズ， エルエルシー ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー
CA2653095C (en) 2006-05-22 2013-07-16 Nanostring Technologies, Inc. Systems and methods for analyzing nanoreporters
JP2009538146A (ja) 2006-05-23 2009-11-05 モレキュラー ディテクション，インコーポレイテッド 室温で安定な分子診断用キット
US7608399B2 (en) 2006-06-26 2009-10-27 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
WO2008036614A1 (en) 2006-09-18 2008-03-27 California Institute Of Technology Apparatus for detecting target molecules and related methods
EP2061901A2 (en) 2006-10-31 2009-05-27 Noxxon Pharma AG Methods for detection of a single- or double-stranded nucleic acid molecule
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
US7930106B2 (en) 2006-11-30 2011-04-19 Gen-Probe Incorporated Quantitative method employing adjustment of pre-defined master calibration curves
CN102851369B (zh) * 2006-12-13 2015-01-21 卢米耐克斯公司 用于实时pcr多重分析的系统和方法
CA2580589C (en) 2006-12-19 2016-08-09 Fio Corporation Microfluidic detection system
US20090042735A1 (en) 2007-03-05 2009-02-12 Blair Steven M Methods and Compositions Related to Nucleic Acid Detection
WO2008118998A2 (en) 2007-03-27 2008-10-02 Primera Biosystems Inc. Method for multiplex detection and quantitation of nucleic acids
AU2008237018B2 (en) 2007-04-10 2014-04-03 Bruker Spatial Biology, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
EP2167537A2 (en) 2007-07-03 2010-03-31 Dako Denmark A/S Compiled methods for analysing and sorting samples
CN101821609A (zh) * 2007-08-29 2010-09-01 艾本德股份有限公司 用于多个样品的辐射度测量的设备和方法
CA2697532A1 (en) 2007-09-20 2009-03-26 Molecular Plant Breeding Nominees Ltd. Method of amplifying nucleic acid
JPWO2009081967A1 (ja) 2007-12-26 2011-05-06 アークレイ株式会社 標的核酸配列の増幅方法およびそれに用いるプローブ
EP2116614A1 (en) 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
GB0812862D0 (en) 2008-07-14 2008-08-20 Lumora Ltd Nucleic acid amplification
US9234232B2 (en) 2008-07-31 2016-01-12 Oxitec Limited Multiplex amplification and detection
JP2011530296A (ja) * 2008-08-08 2011-12-22 スミスズ ディテクション インク． Ｐｃｒアッセイのための検出アルゴリズム
ES2684317T3 (es) 2008-08-14 2018-10-02 Nanostring Technologies, Inc Nanoinformadores estables
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
JP2012509078A (ja) * 2008-11-21 2012-04-19 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 二本鎖核酸特異色素を用いる固体表面でのリアルタイムマルチプレックスｐｃｒ検出
EP2370596B1 (en) 2008-12-01 2017-06-07 QIAGEN GmbH Novel combination of fluorescent dyes for the detection of nucleic acids
EP2376659B1 (en) 2008-12-17 2015-12-02 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
JP5840950B2 (ja) * 2008-12-22 2016-01-06 ユニバーシティ・オブ・ユタ・リサーチ・ファウンデイション 単色マルチプレックス定量ｐｃｒ
US8361738B2 (en) 2009-03-06 2013-01-29 California Institute Of Technology Methods for quantitative target detection and related devices and systems
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
CN101570782B (zh) 2009-03-20 2012-01-11 曹际娟 乳制品中8种致病菌检测试剂盒及其检测方法
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
WO2010130877A2 (en) 2009-05-12 2010-11-18 Abacus Diagnostica Oy Method for detecting nucleic acids
CN102449166B (zh) 2009-05-26 2013-12-04 厦门大学 一种单管检测多个单核苷酸变异或单核苷酸多态性的方法
US8691504B2 (en) 2009-05-26 2014-04-08 Xiamen University Method for detecting variations in nucleic acid sequences
EP2280080A1 (en) 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of nucleic acids using anchor oligonucleotides and adapter oligonucleotides
DK2475788T3 (en) 2009-09-10 2018-07-30 Diasorin S P A COMPENSATION FOR SPECTRAL CRYSTAL SAMPLES IN MULTIPLEX NUCLEIC ACID AMPLIFICATION
US9447457B2 (en) 2009-09-24 2016-09-20 Seegene, Inc. Detection of target nucleic acid sequences by cyclic exonucleolytic reactions
US8962251B2 (en) 2009-10-08 2015-02-24 Medical Service Consultation International, Llc Methods and compositions for identifying sulfur and iron modifying bacteria
WO2011047087A2 (en) 2009-10-13 2011-04-21 Nanostring Technologies, Inc. Protein detection via nanoreporters
US9752187B2 (en) 2009-12-11 2017-09-05 Nucleix Categorization of DNA samples
US9714446B2 (en) 2010-02-11 2017-07-25 Nanostring Technologies, Inc. Compositions and methods for the detection of small RNAs
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
JP5934657B2 (ja) 2010-02-12 2016-06-15 レインダンス テクノロジーズ， インコーポレイテッド デジタル検体分析
WO2011116088A2 (en) 2010-03-16 2011-09-22 Nanostring Technologies, Inc. Compositions and methods for the detection of genomic features
US9422593B2 (en) 2010-05-19 2016-08-23 Qiagen Gaithresburg, Inc Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
CN102269759B (zh) * 2010-06-07 2014-05-07 常州楚天生物科技有限公司 一种基于免疫pcr和dna熔解曲线分析的多重检测方法
EP2588629B1 (en) 2010-06-30 2017-05-17 Gen-Probe Incorporated Method and apparatus for identifying analyte-containing samples using single-read determination of analyte and process control signals
US8877464B2 (en) 2010-07-29 2014-11-04 Roche Molecular Systems, Inc. Qualitative and quantitative detection of microbial nucleic acids
GB201017978D0 (en) * 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
CN107502660B (zh) 2011-01-20 2021-08-10 华盛顿大学商业中心 进行数字测量的方法和系统
US20150140554A1 (en) 2011-02-03 2015-05-21 Richard O. Snyder Detection Methods for Target DNA
WO2012129115A2 (en) 2011-03-18 2012-09-27 University Of South Florida Materials and methods for profiling micrornas
AU2012231098B2 (en) 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US9758834B2 (en) 2011-03-28 2017-09-12 Nanostring Technologies, Inc. Compositions and methods for diagnosing cancer
EP2723897A4 (en) 2011-06-24 2015-03-18 Nanostring Technologies Inc Multivariate diagnostic tests and methods for their use
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US8858623B2 (en) 2011-11-04 2014-10-14 Valtech Cardio, Ltd. Implant having multiple rotational assemblies
CN102559868B (zh) * 2011-11-28 2014-11-05 厦门大学 一种单管定性并定量检测多个目标核酸序列的方法
EP4491741B1 (en) 2011-12-22 2025-10-15 President And Fellows Of Harvard College Compositions and methods for analyte detection
CN107326085B (zh) 2012-02-03 2022-05-03 加州理工学院 多路生化测定中信号的编码和解码
CA2877933A1 (en) 2012-06-27 2014-01-03 Yuri Bushkin Rapid assays for t-cell activation by rna measurements using flow cytometry
IN2015DN01609A (enExample) 2012-08-03 2015-07-03 California Inst Of Techn
GB201218909D0 (en) 2012-10-22 2012-12-05 Univ Singapore Assay for the parallel detection of biological material based on PCR
JP2016509480A (ja) 2013-01-24 2016-03-31 カリフォルニア インスティチュート オブ テクノロジー 発色団に基づく特徴づけおよび検出方法
US11254977B2 (en) 2013-03-12 2022-02-22 Life Technologies Corporation Universal reporter-based genotyping methods and materials
EP2873740A1 (en) 2013-11-19 2015-05-20 Oncodiag Methods for the surveillance, diagnosis and screening of bladder cancer
US9670530B2 (en) 2014-01-30 2017-06-06 Illumina, Inc. Haplotype resolved genome sequencing
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
US10801067B2 (en) 2014-05-09 2020-10-13 Eurofins Lifecodexx Gmbh Detection of DNA that originates from a specific cell-type and related methods
JP2019513345A (ja) 2016-02-10 2019-05-30 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン 核酸の検知
EP4219519A3 (en) 2016-04-01 2023-09-13 Chromacode, Inc. Competitive probes for engineering signal generation
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
MX2018013686A (es) 2016-10-24 2019-05-02 Two Pore Guys Inc Abundancia fraccional de secuencias de polinucleotidos en una muestra.
WO2019006023A1 (en) 2017-06-28 2019-01-03 ChromaCode, Inc. MULTIPLEXED FLUORIMETRIC MEASUREMENTS WITH DROPLET PCR SYSTEMS
US20190112636A1 (en) 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
US20200407794A1 (en) 2018-02-28 2020-12-31 ChromaCode, Inc. Molecular targets for fetal nucleic acid analysis

Also Published As

Publication number Publication date
EP2880184A1 (en) 2015-06-10
EP2880184A4 (en) 2016-03-30
CN108753932B (zh) 2022-12-02
EP2880184B1 (en) 2021-03-31
US20180052110A1 (en) 2018-02-22
WO2014022827A1 (en) 2014-02-06
CN108753932A (zh) 2018-11-06
US20140038195A1 (en) 2014-02-06
CN104662172B (zh) 2018-07-06
CN104662172A (zh) 2015-05-27
US20240201090A1 (en) 2024-06-20
EP3901243A1 (en) 2021-10-27
US11959856B2 (en) 2024-04-16
US9791372B2 (en) 2017-10-17

Similar Documents

Publication Publication Date Title
MY194686A (en) Detection of chromosome interactions
CA2840149C (en) Methods and nucleic acids for determining the prognosis of a cancer subject
EP4524972A3 (en) Analysis of genetic variants
EP3816303A3 (en) Breast cancer detection kit or device, and method for detecting breast cancer
IN2014DN10121A (enExample)
EP3757225A3 (en) Pancreatic cancer detection kit or device, and detection method
EP3071333A4 (en) Cartridges and instruments for sample analysis
EP3800253A3 (en) Prostate cancer detection kit or device, and detection method
GB201305223D0 (en) Reflection detection type measurement apparatus for skin autofluorescence
EP2813029A4 (en) SYSTEM AND METHOD FOR CREATING AND PROTECTING CRYPTOGRAPHIC KEYS
GB2497693A (en) Managing a user interface for an application program
EP2614511A4 (en) PRESSURE MEASURING DEVICES AND METHOD
EP3608021A3 (en) Analyte detection devices, multiplex and tabletop devices for detection of analytes, and uses thereof
MX2018004074A (es) Sistemas y metodos para el ajuste de dispositivos.
GB2502715A (en) Malware Detection
EP2695127A4 (en) SYSTEM AND METHOD FOR AUDITING AUDIENTS BASED ON UNIT-COMPONENT CONTEXTUAL ENTRIES
BR112012026536A2 (pt) sistemas e métodos para predizer deficiência gastrointestinal
CL2015001568A1 (es) Métodos de detección multiplex de ácidos nucleicos
GB2496557A (en) Products and methods for identifying rock samples
EP3798316A3 (en) Genes and gene signatures for diagnosis and treatment of melanoma
EP3058097A4 (en) Sense-antisense gene pairs for patient stratification, prognosis, and therapeutic biomarkers identification
NZ629555A (en) Monocyte biomarkers for cancer detection
WO2012075069A3 (en) Signatures and determinants associated with cancer and methods of use thereof
GB2545850A (en) System and method using a combined modality optical probe
EP3001410A4 (en) MUSIC INTERPRETATION RECORDING SYSTEM, MUSICAL INTERPRETATION RECORDING METHOD, AND MUSIC INSTRUMENT