JP2018042563A5 - - Google Patents

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Publication number
JP2018042563A5
JP2018042563A5 JP2017224810A JP2017224810A JP2018042563A5 JP 2018042563 A5 JP2018042563 A5 JP 2018042563A5 JP 2017224810 A JP2017224810 A JP 2017224810A JP 2017224810 A JP2017224810 A JP 2017224810A JP 2018042563 A5 JP2018042563 A5 JP 2018042563A5
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Japan
Prior art keywords
protein
genetic element
molecule
gene product
aqueous
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JP2017224810A
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English (en)
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JP2018042563A (ja
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Priority claimed from US10/961,695 external-priority patent/US7968287B2/en
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Publication of JP2018042563A publication Critical patent/JP2018042563A/ja
Publication of JP2018042563A5 publication Critical patent/JP2018042563A5/ja
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Claims (14)

  1. 化合物を分析するための方法であって、
    マイクロ流体制御下で油中に複数の水性マイクロカプセルを形成させる工程、ここに、各水性マイクロカプセルは遺伝要素および細胞を含む;
    前記第1の遺伝要素が前記細胞と関連した分子と相互作用するように、前記複数の水性マイクロカプセルをインキュベートする工程;
    前記水性マイクロカプセルの1つ以上の内部で前記細胞と関連した分子が関与する増幅反応を実施する工程、ここに、前記遺伝要素または前記遺伝要素によりコードされる遺伝子産物が前記増幅反応に影響する;および
    前記水性マイクロカプセルの1つ以上における増幅反応の産物によってひき起こされる選択可能な変化を検出する工程
    を含む、方法。
  2. 複数の水性マイクロカプセルが、界面活性剤の存在に起因して融合または合体できない、請求項1に記載の方法。
  3. 選択可能な変化が光学的シグナルを含む、請求項1に記載の方法。
  4. 遺伝要素が増幅のための1つ以上のプライマ配列を含む、請求項1に記載の方法。
  5. 遺伝要素がビーズに付着されている、請求項1に記載の方法。
  6. 分子がレポータタンパク質である、請求項1に記載の方法。
  7. 分子が遺伝子を含む、請求項1に記載の方法。
  8. 遺伝子産物を形成させるために遺伝子を発現させる工程をさらに含む、請求項7に記載の方法。
  9. 遺伝子産物がタンパク質である、請求項8に記載の方法。
  10. タンパク質が抗体である、請求項9に記載の方法。
  11. タンパク質が細胞内に位置する、請求項9に記載の方法。
  12. タンパク質が細胞外に位置する、請求項9に記載の方法。
  13. 分子がDNAまたはRNAである、請求項1に記載の方法。
  14. 遺伝子産物がcDNAである、請求項8に記載の方法。
JP2017224810A 2004-10-08 2017-11-22 マイクロ流体システム内のインビトロ進化 Pending JP2018042563A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/961,695 2004-10-08
US10/961,695 US7968287B2 (en) 2004-10-08 2004-10-08 In vitro evolution in microfluidic systems

Related Parent Applications (1)

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JP2015235625A Division JP2016063824A (ja) 2004-10-08 2015-12-02 マイクロ流体システム内のインビトロ進化

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JP2019043813A Division JP2019122394A (ja) 2004-10-08 2019-03-11 マイクロ流体システム内のインビトロ進化

Publications (2)

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JP2018042563A JP2018042563A (ja) 2018-03-22
JP2018042563A5 true JP2018042563A5 (ja) 2018-05-10

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JP2007535247A Pending JP2008515421A (ja) 2004-10-08 2005-10-10 マイクロ流体システム内のインビトロ進化
JP2013077141A Active JP5903067B2 (ja) 2004-10-08 2013-04-02 マイクロ流体システム内のインビトロ進化
JP2015235625A Pending JP2016063824A (ja) 2004-10-08 2015-12-02 マイクロ流体システム内のインビトロ進化
JP2017224810A Pending JP2018042563A (ja) 2004-10-08 2017-11-22 マイクロ流体システム内のインビトロ進化
JP2019043813A Withdrawn JP2019122394A (ja) 2004-10-08 2019-03-11 マイクロ流体システム内のインビトロ進化
JP2021165419A Pending JP2022068841A (ja) 2004-10-08 2021-10-07 マイクロ流体システム内のインビトロ進化

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JP2007535247A Pending JP2008515421A (ja) 2004-10-08 2005-10-10 マイクロ流体システム内のインビトロ進化
JP2013077141A Active JP5903067B2 (ja) 2004-10-08 2013-04-02 マイクロ流体システム内のインビトロ進化
JP2015235625A Pending JP2016063824A (ja) 2004-10-08 2015-12-02 マイクロ流体システム内のインビトロ進化

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JP2019043813A Withdrawn JP2019122394A (ja) 2004-10-08 2019-03-11 マイクロ流体システム内のインビトロ進化
JP2021165419A Pending JP2022068841A (ja) 2004-10-08 2021-10-07 マイクロ流体システム内のインビトロ進化

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US (9) US7968287B2 (ja)
EP (6) EP2949761A1 (ja)
JP (6) JP2008515421A (ja)
AT (1) ATE527381T1 (ja)
AU (1) AU2005291025A1 (ja)
CA (1) CA2582476C (ja)
WO (1) WO2006038035A2 (ja)

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