DE69615411T2 - Verfahren zur Herstellung von Polynucleotiden mit erwünschten Eigenschaften mittels wiederholter Selektion und Rekombination - Google Patents

Verfahren zur Herstellung von Polynucleotiden mit erwünschten Eigenschaften mittels wiederholter Selektion und Rekombination

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Publication number
DE69615411T2
DE69615411T2 DE69615411T DE69615411T DE69615411T2 DE 69615411 T2 DE69615411 T2 DE 69615411T2 DE 69615411 T DE69615411 T DE 69615411T DE 69615411 T DE69615411 T DE 69615411T DE 69615411 T2 DE69615411 T2 DE 69615411T2
Authority
DE
Germany
Prior art keywords
sequence
recombination
polynucleotide
dna
sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69615411T
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German (de)
English (en)
Other versions
DE69615411D1 (de
Inventor
Andreas Crameri
Willem P.C. Stemmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Maxygen Inc
Original Assignee
Maxygen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27073702&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69615411(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US08/564,955 external-priority patent/US5811238A/en
Application filed by Maxygen Inc filed Critical Maxygen Inc
Publication of DE69615411D1 publication Critical patent/DE69615411D1/de
Application granted granted Critical
Publication of DE69615411T2 publication Critical patent/DE69615411T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/02006—Beta-lactamase (3.5.2.6)
    • H—ELECTRICITY
    • H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10N—ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10N60/00—Superconducting devices
    • H10N60/10—Junction-based devices
    • H10N60/128—Junction-based devices having three or more electrodes, e.g. transistor-like structures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

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  • Chemical & Material Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE69615411T 1995-11-30 1996-12-02 Verfahren zur Herstellung von Polynucleotiden mit erwünschten Eigenschaften mittels wiederholter Selektion und Rekombination Expired - Fee Related DE69615411T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/564,955 US5811238A (en) 1994-02-17 1995-11-30 Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US08/621,859 US6117679A (en) 1994-02-17 1996-03-25 Methods for generating polynucleotides having desired characteristics by iterative selection and recombination

Publications (2)

Publication Number Publication Date
DE69615411D1 DE69615411D1 (de) 2001-10-25
DE69615411T2 true DE69615411T2 (de) 2002-06-20

Family

ID=27073702

Family Applications (4)

Application Number Title Priority Date Filing Date
DE69615411T Expired - Fee Related DE69615411T2 (de) 1995-11-30 1996-12-02 Verfahren zur Herstellung von Polynucleotiden mit erwünschten Eigenschaften mittels wiederholter Selektion und Rekombination
DE0876509T Pending DE876509T1 (de) 1995-11-30 1996-12-02 Verfahren zur herstellung von polynukleotiden mit gewünschten eigenschaftendurch iterative selektion und rekombination
DE0911396T Pending DE911396T1 (de) 1995-11-30 1996-12-02 Verfahren zur Herstellung von Polynucleotiden mit erwünschten Eigenschaften mittels wiederholter Selektion und Rekombination
DE69615406T Expired - Lifetime DE69615406T3 (de) 1995-11-30 1996-12-02 Verfahren zur herstellung von polynukleotiden mit gewünschten eigenschaften durch iterative selektion und rekombination

Family Applications After (3)

Application Number Title Priority Date Filing Date
DE0876509T Pending DE876509T1 (de) 1995-11-30 1996-12-02 Verfahren zur herstellung von polynukleotiden mit gewünschten eigenschaftendurch iterative selektion und rekombination
DE0911396T Pending DE911396T1 (de) 1995-11-30 1996-12-02 Verfahren zur Herstellung von Polynucleotiden mit erwünschten Eigenschaften mittels wiederholter Selektion und Rekombination
DE69615406T Expired - Lifetime DE69615406T3 (de) 1995-11-30 1996-12-02 Verfahren zur herstellung von polynukleotiden mit gewünschten eigenschaften durch iterative selektion und rekombination

Country Status (11)

Country Link
US (8) US6117679A (enExample)
EP (4) EP0876509B2 (enExample)
JP (3) JP4015193B2 (enExample)
AT (2) ATE205878T1 (enExample)
AU (1) AU713952B2 (enExample)
CA (1) CA2239099C (enExample)
DE (4) DE69615411T2 (enExample)
DK (2) DK0876509T4 (enExample)
ES (2) ES2165124T3 (enExample)
IL (1) IL124675A0 (enExample)
WO (1) WO1997020078A1 (enExample)

Families Citing this family (650)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6808904B2 (en) 1994-06-16 2004-10-26 Syngenta Participations Ag Herbicide-tolerant protox genes produced by DNA shuffling
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6562594B1 (en) 1999-09-29 2003-05-13 Diversa Corporation Saturation mutagenesis in directed evolution
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US20020028443A1 (en) * 1999-09-27 2002-03-07 Jay M. Short Method of dna shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US7018793B1 (en) 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6764835B2 (en) 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6096548A (en) * 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6696251B1 (en) 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6773910B1 (en) 1996-07-10 2004-08-10 Walter Brieden Method of preparing (S)- or (R) -3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid
US6025485A (en) 1997-02-14 2000-02-15 Arcaris, Inc. Methods and compositions for peptide libraries displayed on light-emitting scaffolds
US5955275A (en) * 1997-02-14 1999-09-21 Arcaris, Inc. Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
US7148054B2 (en) * 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
WO1998031837A1 (en) 1997-01-17 1998-07-23 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
GB9701425D0 (en) 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6623922B1 (en) 1997-02-14 2003-09-23 Deltagen Proteomics Methods for identifying, characterizing, and evolving cell-type specific CIS regulatory elements
US6159687A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods for generating recombined polynucleotides
US6159688A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods of producing polynucleotide variants
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
ATE280221T1 (de) 1997-04-09 2004-11-15 Danisco Verwendung von lipase zur verbesserung von teigen und backwaren
US6046925A (en) * 1997-04-14 2000-04-04 The Regents Of The University Of California Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins
GB9712512D0 (en) 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
DE19725371B4 (de) * 1997-06-16 2005-01-20 Bornholdt, Stefan, Dr. Verfahren zur Beschleunigung der evolutiven Optimierung von Biopolymeren und zur Verbesserung der damit hergestellten Biopolymere
DE19737562A1 (de) * 1997-08-28 1999-05-06 Otogene Biotechnologische Fors Verfahren zur Identifizierung von Wechselwirkungen zwischen Proteinen bzw. Peptiden
GB9722131D0 (en) * 1997-10-20 1997-12-17 Medical Res Council Method
AU1124499A (en) 1997-10-28 1999-05-17 Maxygen, Inc. Human papillomavirus vectors
US6596539B1 (en) * 1997-10-31 2003-07-22 Maxygen, Inc. Modification of virus tropism and host range by viral genome shuffling
US6156509A (en) * 1997-11-12 2000-12-05 Genencor International, Inc. Method of increasing efficiency of directed evolution of a gene using phagemid
EP2270234B1 (en) 1997-12-08 2013-03-06 California Institute of Technology Method for creating polynucleotide and polypeptide sequences
AR017831A1 (es) 1997-12-10 2001-10-24 Pioneer Hi Bred Int Metodo para alterar la composicion de aminoacidos de una proteina nativa de interes, proteina elaborada, y polinucleotido
EP1555316A3 (en) * 1998-02-11 2008-01-30 Maxygen, Inc. Antigen library immunization
JP2002507393A (ja) * 1998-02-11 2002-03-12 マキシジェン， インコーポレイテッド 抗原ライブラリー免疫
EP1518927A3 (en) * 1998-02-11 2005-06-22 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
US6541011B2 (en) 1998-02-11 2003-04-01 Maxygen, Inc. Antigen library immunization
JP2002503461A (ja) * 1998-02-11 2002-02-05 マキシジェン， インコーポレイテッド 遺伝子ワクチンベクター工学
DE19805334A1 (de) * 1998-02-11 1999-08-12 Otogene Biotechnologische Fors Verfahren zur Entwicklung eines pharmazeutischen Wirkstoffes
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
AU2535599A (en) * 1998-02-17 1999-08-30 Zeneca Limited Insecticidal peptides
KR20010082543A (ko) 1998-05-01 2001-08-30 추후제출 Ｄｎａ 재편성을 이용한 내병충성 유전자의 최적화 방법
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
US7153655B2 (en) * 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
KR20010052894A (ko) 1998-06-17 2001-06-25 맥시겐, 인크. 목적하는 특성을 보유한 폴리뉴클레오티드를 생성하는 방법
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
IL140100A0 (en) * 1998-06-29 2002-02-10 Phylos Inc Methods of generating highly diverse libraries
DE69904941T3 (de) 1998-07-21 2008-01-31 Danisco A/S Lebensmittel
JP2003503005A (ja) * 1998-07-28 2003-01-28 カリフォルニア インスティチュート オブ テクノロジー 機能的真核生物タンパク質の発現
US20040191772A1 (en) * 1998-08-12 2004-09-30 Dupret Daniel Marc Method of shuffling polynucleotides using templates
US6991922B2 (en) * 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US20030104417A1 (en) * 1998-08-12 2003-06-05 Proteus S.A. Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides
US20020058249A1 (en) * 1998-08-12 2002-05-16 Venkiteswaran Subramanian Dna shuffling to produce herbicide selective crops
US20030092023A1 (en) * 1998-08-12 2003-05-15 Daniel Dupret Method of shuffling polynucleotides using templates
EP1104459A1 (en) 1998-08-12 2001-06-06 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
US6951719B1 (en) * 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
JP4704565B2 (ja) 1998-09-23 2011-06-15 ザイモジェネティクス， インコーポレイテッド サイトカインレセプターｚａｌｐｈａ１１
EP1119616A2 (en) * 1998-10-07 2001-08-01 Maxygen, Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
EP1129182A1 (en) * 1998-11-10 2001-09-05 Maxygen, Inc. Modified ribulose 1,5-bisphosphate carboxylase/oxygenase
EP1129184A1 (en) 1998-11-10 2001-09-05 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimation of plant phenotypes
US6438561B1 (en) * 1998-11-19 2002-08-20 Navigation Technologies Corp. Method and system for using real-time traffic broadcasts with navigation systems
IT1303776B1 (it) * 1998-11-19 2001-02-23 S I S S A Scuola Internaz Supe Processo per la preparazione di genoteche, di polipeptidi utilizzandodette genoteche e i polipepti ottenuti.
US20040005673A1 (en) * 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
DE60042730D1 (de) 1999-01-05 2009-09-24 Univ Boston Verbessertes klonierungsverfahren
DK1642972T3 (da) 1999-01-07 2010-05-10 Zymogenetics Inc Terapeutisk anvendelse af BR43X2 opløselige receptorer
CA2337949C (en) * 1999-01-18 2011-03-15 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20030054390A1 (en) * 1999-01-19 2003-03-20 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) * 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
EP1151409A1 (en) * 1999-01-18 2001-11-07 Maxygen, Inc. Methods of populating data stuctures for use in evolutionary simulations
US7873477B1 (en) 2001-08-21 2011-01-18 Codexis Mayflower Holdings, Llc Method and system using systematically varied data libraries
KR20010042037A (ko) * 1999-01-19 2001-05-25 맥시겐, 인크. 목적으로 하는 특징을 갖는 문자열인, 폴리뉴클레오타이드및 폴리펩타이드의 제작 방법
US20070065838A1 (en) * 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
EP1108783A3 (en) * 1999-01-19 2001-09-05 Maxygen, Inc. Oligonucleotide-mediated nucleic acid recombination
US7024312B1 (en) * 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US8457903B1 (en) 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
MXPA00009723A (es) * 1999-02-04 2003-09-22 Diversa Corp Generacion no estocastica de vacunas y enzimas geneticas.
US20090130718A1 (en) * 1999-02-04 2009-05-21 Diversa Corporation Gene site saturation mutagenesis
US7384387B1 (en) 1999-02-11 2008-06-10 Maxygen, Inc. High throughput mass spectrometry
JP3399518B2 (ja) * 1999-03-03 2003-04-21 インターナショナル・ビジネス・マシーンズ・コーポレーション 半導体構造およびその製造方法
CA2362737A1 (en) 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
AU3879300A (en) * 1999-03-09 2000-09-28 Diversa Corporation End selection in directed evolution
EP2295577A3 (en) 1999-03-09 2012-08-08 ZymoGenetics, Inc. Human cytokine as ligand of the z-alpha receptor and uses thereof
US8148110B2 (en) * 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
AU2005225057A1 (en) * 1999-03-26 2005-12-01 Bp Corporation North America Inc. Synthetic ligation reassembly in directed evolution
CA2329122A1 (en) * 1999-03-26 2000-10-05 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US20040137515A1 (en) * 1999-06-21 2004-07-15 Gary Lynch Methods and device for in vitro detection and characterization of psychoactives using analysis of repetitive electrical activity in a neuronal sample
JP2003503039A (ja) * 1999-06-25 2003-01-28 マキシジェン， インコーポレイテッド 弱毒化したワクチンの操作のための方法および組成物
WO2001004287A1 (en) * 1999-07-07 2001-01-18 Maxygen Aps A method for preparing modified polypeptides
ES2286029T3 (es) 1999-07-07 2007-12-01 Zymogenetics Inc Receptor de citocina humano.
US7179638B2 (en) 1999-07-30 2007-02-20 Large Scale Biology Corporation Microarrays and their manufacture by slicing
US6713309B1 (en) 1999-07-30 2004-03-30 Large Scale Proteomics Corporation Microarrays and their manufacture
NZ516517A (en) 1999-08-12 2003-10-31 Pfizer Prod Inc Mutations in the aveC allele of Streptomyces avermitilis gene that reduce the ratio of cyclohexyl B2:cyclohexyl B1 avermectins
US6586230B1 (en) * 2000-10-27 2003-07-01 Lexicon Genetics Incorporated Human kinase and polynucleotides encoding the same
US6797510B1 (en) * 2001-05-24 2004-09-28 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US20080050809A1 (en) * 1999-09-28 2008-02-28 Alejandro Abuin Novel human kinases and polynucleotides encoding the same
US20040002474A1 (en) * 1999-10-07 2004-01-01 Maxygen Inc. IFN-alpha homologues
US7430477B2 (en) * 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
WO2001032829A2 (en) * 1999-11-05 2001-05-10 Novozymes A/S Methods to screen microorganisms or gene libraries for products secreted from a cell
DE19953854C2 (de) 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
EP2194130A1 (en) 1999-12-23 2010-06-09 ZymoGenetics, L.L.C. Cytokine ZCYTO18
ES2327606T3 (es) 2000-01-10 2009-11-02 Maxygen Holdings Ltd Conjugados de g-csf.
IL150291A0 (en) * 2000-01-11 2002-12-01 Maxygen Inc Integrated systems and methods for diversity generation and screening
MXPA02007078A (es) 2000-02-09 2003-03-27 Basf Ag Nuevo gen de elongasa y procedimiento para la obtencion de acidos grasos poliinsaturados.
WO2001058935A2 (en) 2000-02-11 2001-08-16 Maxygen Aps FACTOR VII OR VIIa-LIKE MOLECULES
WO2001064864A2 (en) * 2000-02-28 2001-09-07 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
AU2001247676A1 (en) * 2000-03-21 2001-10-03 Neugenesis Corporation Methods for in vivo diversification of single genes
US20010049104A1 (en) * 2000-03-24 2001-12-06 Stemmer Willem P.C. Methods for modulating cellular and organismal phenotypes
WO2001075767A2 (en) * 2000-03-30 2001-10-11 Maxygen, Inc. In silico cross-over site selection
EP1303602B1 (en) 2000-04-05 2006-12-27 ZymoGenetics, Inc. Soluble zalpha11 cytokine receptors
US7115403B1 (en) 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US20020045175A1 (en) * 2000-05-23 2002-04-18 Zhen-Gang Wang Gene recombination and hybrid protein development
US20030032059A1 (en) * 2000-05-23 2003-02-13 Zhen-Gang Wang Gene recombination and hybrid protein development
US6686188B2 (en) 2000-05-26 2004-02-03 Amersham Plc Polynucleotide encoding a human myosin-like polypeptide expressed predominantly in heart and muscle
EP1360290A2 (en) * 2000-06-23 2003-11-12 Maxygen, Inc. Co-stimulatory molecules
AU2001268716A1 (en) 2000-06-23 2002-01-08 Maxygen, Inc. Novel chimeric promoters
AU2001273032A1 (en) 2000-06-26 2002-01-08 Zymogenetics Inc. Cytokine receptor zcytor17
EP2281878A1 (en) 2000-06-26 2011-02-09 Novozymes A/S Lipolytic enzyme
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
AU2001271912A1 (en) * 2000-07-07 2002-01-21 Maxygen, Inc. Molecular breeding of transposable elements
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US6858422B2 (en) * 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
US7087415B2 (en) * 2000-07-31 2006-08-08 Athena Biotechnologies, Inc. Methods and compositions for directed gene assembly
US20020132308A1 (en) * 2000-08-24 2002-09-19 Mpep @ Page 300-M Novel constructs and their use in metabolic pathway engineering
AU2001288478B2 (en) 2000-08-25 2006-11-02 Basf Plant Science Gmbh Plant polynucleotides encoding prenyl proteases
WO2002022810A2 (en) * 2000-09-15 2002-03-21 Diversa Corporation Combinatorial screening of mixed populations of organisms
AU2002213183A1 (en) 2000-10-12 2002-04-22 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US6912470B2 (en) * 2001-05-21 2005-06-28 Ecopia Biosciences, Inc. Genes and proteins involved in the biosynthesis of enediyne ring structures
US7045283B2 (en) 2000-10-18 2006-05-16 The Regents Of The University Of California Methods of high-throughput screening for internalizing antibodies
US7202070B2 (en) * 2000-10-31 2007-04-10 Biocatalytics, Inc. Method for reductive amination of a ketone using a mutated enzyme
WO2002042438A2 (en) 2000-11-20 2002-05-30 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US6958213B2 (en) * 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
WO2002048333A2 (en) * 2000-12-12 2002-06-20 Lexicon Genetics Incorporated Novel human kinases and uses thereof
PT1341909E (pt) * 2000-12-12 2005-10-31 Alligator Bioscience Ab Metodo para a evolucao molecular in vitro da funcao de proteina
GB2370038B (en) * 2000-12-12 2003-03-26 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
AU2002228974A1 (en) * 2000-12-13 2002-06-24 Nugen Technologies, Inc Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
US20020150895A1 (en) * 2000-12-22 2002-10-17 Raymond Wheeler Method and apparatus for filtering and extending RNA alignment coverage
WO2002059325A2 (en) * 2000-12-27 2002-08-01 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US6849443B2 (en) * 2001-01-23 2005-02-01 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
EP1356037B1 (en) * 2001-01-25 2011-03-09 Evolva Ltd. A library of a collection of cells
JP2004517638A (ja) * 2001-02-02 2004-06-17 マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファオ 機能的核酸の同定方法
US20050003354A1 (en) * 2001-02-21 2005-01-06 Maxygen, Inc. Methods for improving or altering promoter/enhancer properties
WO2002072772A2 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
WO2002074932A2 (en) 2001-03-20 2002-09-26 Lexicon Genetics Incorporated Novel human kinase and polynucleotides encoding the same
US20030087254A1 (en) * 2001-04-05 2003-05-08 Simon Delagrave Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics
CA2443516A1 (en) * 2001-04-06 2002-10-17 Lexicon Genetics Incorporated Novel human kinases and polynucleotides encoding the same
WO2002083868A2 (en) 2001-04-16 2002-10-24 California Institute Of Technology Peroxide-driven cytochrome p450 oxygenase variants
AU2002258997A1 (en) * 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
JP2004528850A (ja) * 2001-05-03 2004-09-24 レンセレアー ポリテクニック インスティテュート 定方向進化の新規方法
EP1463749A4 (en) 2001-05-09 2005-04-13 Lexicon Genetics Inc NEW HUMAN KINASES AND THESE CODING POLYNUCLEOTIDES
CA2444960C (en) 2001-05-18 2011-08-09 Danisco A/S Method of improving dough and bread quality
CA2447240C (en) * 2001-05-18 2013-02-19 Wisconsin Alumni Research Foundation Method for the synthesis of dna sequences
EP2298868B1 (en) 2001-06-26 2015-01-07 Novozymes A/S Polypeptides having cellobiohydrolase I activity and polynucleotides encoding same
US20030143612A1 (en) * 2001-07-18 2003-07-31 Pointilliste, Inc. Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
WO2003008563A2 (en) 2001-07-20 2003-01-30 California Institute Of Technology Improved cytochrome p450 oxygenases
DE60210096D1 (de) * 2001-07-26 2006-05-11 Ecopia Biosciences Inc Gene und proteine zur rosaramicin biosynthese
US8067556B2 (en) * 2001-07-26 2011-11-29 Agilent Technologies, Inc. Multi-site mutagenesis
US6833441B2 (en) 2001-08-01 2004-12-21 Abmaxis, Inc. Compositions and methods for generating chimeric heteromultimers
US20040078837A1 (en) * 2001-08-02 2004-04-22 Shannon Mark E. Four human zinc-finger-containing proteins: MDZ3, MDZ4, MDZ7 and MDZ12
CA2456229A1 (en) * 2001-08-03 2003-02-13 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods for making and using them
US6943001B2 (en) * 2001-08-03 2005-09-13 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods for making and using them
US6987079B2 (en) * 2001-08-14 2006-01-17 W.R. Grace & Co.-Conn. Supported catalyst systems
US7647184B2 (en) * 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
MXPA04002817A (es) 2001-09-27 2004-07-05 Pioneer Hi Bred Int Polinucleotidos de fitato y metodos de uso.
WO2003029456A1 (en) 2001-10-01 2003-04-10 Dyax Corp. Multi-chain eukaryotic display vectors and uses thereof
NZ532027A (en) 2001-10-10 2008-09-26 Neose Technologies Inc Remodeling and glycoconjugation of peptides
WO2003035842A2 (en) * 2001-10-24 2003-05-01 Dyax Corporation Hybridization control of sequence variation
US7175983B2 (en) * 2001-11-02 2007-02-13 Abmaxis, Inc. Adapter-directed display systems
DK1451322T3 (da) 2001-11-05 2010-02-01 Zymogenetics Inc Il-21-antagonister
GB0126887D0 (en) * 2001-11-08 2002-01-02 Univ London Method for producing and identifying soluble protein domains
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
CA2467460A1 (en) * 2001-11-19 2003-05-30 Parallele Bioscience, Inc. Multiplex oligonucleotide addition and target amplification
US6768476B2 (en) * 2001-12-05 2004-07-27 Etenna Corporation Capacitively-loaded bent-wire monopole on an artificial magnetic conductor
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
US20040137547A1 (en) * 2001-12-28 2004-07-15 Medtronic Minimed, Inc. Method for formulating a glucose oxidase enzyme with a desired property or properties and a glucose oxidase enzyme with the desired property
US20040009498A1 (en) * 2002-01-14 2004-01-15 Diversa Corporation Chimeric antigen binding molecules and methods for making and using them
WO2003061570A2 (en) * 2002-01-16 2003-07-31 Zyomyx, Inc. Engineered binding proteins
ES2546797T3 (es) 2002-01-18 2015-09-28 Zymogenetics, Inc. Multímeros Zcytor17 receptores de citocinas
EP1476541B1 (en) 2002-01-18 2008-07-16 ZymoGenetics, Inc. Cytokine (zcytor17 ligand)
US20040048311A1 (en) * 2002-01-24 2004-03-11 Dana Ault-Riche Use of collections of binding sites for sample profiling and other applications
JP4472345B2 (ja) * 2002-01-25 2010-06-02 エヴォルヴァ・リミテッド 複数パラメータースクリーニングおよび複数機能性小分子産生細胞への進化方法
US20040096826A1 (en) * 2002-01-30 2004-05-20 Evans Glen A. Methods for creating recombination products between nucleotide sequences
PL211693B1 (pl) 2002-02-12 2012-06-29 Pfizer Prod Inc Gen Streptomyces Avermitilis kierujący stosunkiem awermektyn B2:B1
ES2310237T3 (es) 2002-02-21 2009-01-01 Asm Scientific, Inc. Amplificacion por recombinasa polimerasa.
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
ATE414142T1 (de) 2002-02-22 2008-11-15 Progenics Pharm Inc Anti-ccr5 antikörper
AU2003217716A1 (en) * 2002-02-25 2003-09-09 Cabot Corporation Custom ligand design for biomolecular filtration and purification for bioseperation
US20030224404A1 (en) * 2002-02-25 2003-12-04 Manuel Vega High throughput directed evolution of nucleic acids by rational mutagenesis
EP1476563B1 (en) 2002-02-26 2008-09-17 E.I. Du Pont De Nemours And Company Method for the recombination of genetic elements
WO2003072742A2 (en) 2002-02-27 2003-09-04 California Institute Of Technology Novel glucose 6-oxidases
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
WO2003075129A2 (en) 2002-03-01 2003-09-12 Maxygen, Inc. Methods, systems, and software for identifying functional bio-molecules
IL163597A0 (en) * 2002-03-01 2005-12-18 Ravgen Inc Rapid analysis of variations in a genome
US7747391B2 (en) * 2002-03-01 2010-06-29 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
WO2003078583A2 (en) 2002-03-09 2003-09-25 Maxygen, Inc. Optimization of crossover points for directed evolution
US7176025B2 (en) * 2002-03-11 2007-02-13 Nugen Technologies, Inc. Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination
CN100497378C (zh) 2002-03-22 2009-06-10 拜尔生物科学公司 新的苏云金芽孢杆菌杀昆虫蛋白质
EP1572927A4 (en) 2002-04-08 2007-10-17 Pioneer Hi Bred Int REINFORCED EXTRACTION OF SILK UNDER STRESS
JP2005523014A (ja) * 2002-04-16 2005-08-04 プロメガ　コーポレイション 相同的組換えの増強方法
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
CN1659274A (zh) 2002-04-19 2005-08-24 津莫吉尼蒂克斯公司 细胞因子受体
ES2395898T3 (es) 2002-04-19 2013-02-15 Dsm Ip Assets B.V. Fosfolipasas, ácidos nucleicos que las codifican, y métodos para obtenerlas y usarlas
EP1501927A2 (en) * 2002-05-02 2005-02-02 University Of North Carolina At Chapel Hill In vitro mutagenesis, phenotyping, and gene mapping
FR2839317A1 (fr) * 2002-05-06 2003-11-07 Entomed Procede d'evolution fonctionnelle artificielle de sequences polynucleotidiques, polynucleotides recombines obtenus et peptides, polypeptides et proteines codes par ceux-ci
US7442506B2 (en) * 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US20070178478A1 (en) * 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
GB2388604B (en) * 2002-05-17 2005-01-26 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
DK1504098T4 (da) * 2002-05-17 2011-05-23 Alligator Bioscience Ab Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion
CA2487133A1 (en) 2002-06-07 2003-12-18 Zymogenetics, Inc. Use of il-21 in cancer and other therapeutic applications
GB0213816D0 (en) * 2002-06-14 2002-07-24 Univ Aston Method of producing DNA and protein libraries
US20030232395A1 (en) * 2002-06-14 2003-12-18 Dyax Corporation Recombination of nucleic acid library members
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
DE10233047A1 (de) * 2002-07-19 2004-02-26 Amaxa Gmbh Verfahren zur Herstellung eines künstlichen Polypeptids und nach diesem Verfahren hergestelltes künstliches Protein
EP1527173A1 (en) * 2002-07-23 2005-05-04 Nuevolution A/S Gene shuffing by template switching
WO2004011605A2 (en) 2002-07-25 2004-02-05 Mj Bioworks Incorporated Hybrid protein methods and compositions
EP1529110B1 (en) * 2002-08-01 2011-10-05 Evolva Ltd. Methods of mixing large numbers of heterologous genes
CA2494798A1 (en) 2002-08-06 2005-01-13 Verdia, Inc. Ap1 amine oxidase variants
US7047591B2 (en) * 2002-09-20 2006-05-23 Colgate-Palmolive Company Oral care implement
AU2003263552A1 (en) * 2002-09-09 2004-03-29 Nautilus Biotech Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules
US20050202438A1 (en) * 2002-09-09 2005-09-15 Rene Gantier Rational directed protein evolution using two-dimensional rational mutagenesis scanning
US20060020396A1 (en) * 2002-09-09 2006-01-26 Rene Gantier Rational directed protein evolution using two-dimensional rational mutagenesis scanning
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
CA2499552C (en) * 2002-09-20 2010-11-16 Colgate-Palmolive Company Toothbrush with gripping area
AU2002951899A0 (en) * 2002-10-04 2002-10-24 Macquarie University Random drift mutagenesis
JP3447009B1 (ja) * 2002-10-29 2003-09-16 實 平垣 構築物用構成体およびその製造方法
EP1576126A3 (en) * 2002-10-30 2005-10-26 Pointilliste, Inc. Systems for capture and analysis of biological particles and methods using the systems
US7135290B2 (en) * 2003-04-12 2006-11-14 Solazyme, Inc. Methods and compositions for evolving hydrogenase genes
US7090979B2 (en) * 2002-11-22 2006-08-15 The Regents Of The University Of California Derivatized versions of ligase enzymes for constructing DNA sequences
DK1578964T4 (da) 2002-12-20 2013-12-02 Novozymes As Polypeptider med cellobiohydrolase II-aktivitet og polynukleotider, der koder for samme
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
DE602004030000D1 (de) 2003-01-17 2010-12-23 Danisco Verfahren zur in-situ-herstellung eines emulgators in einem nahrungsmittel
US20050196766A1 (en) 2003-12-24 2005-09-08 Soe Jorn B. Proteins
ES2661213T3 (es) 2003-01-17 2018-03-28 Dupont Nutrition Biosciences Aps Método para la producción in situ de un emulsionante en un producto alimenticio
EP1585752B1 (en) 2003-01-21 2007-11-21 Thallion Pharmaceuticals Inc. Polyene polyketides, processes for their production and their use as a pharmaceutical
DE102004002243A1 (de) * 2003-02-07 2004-09-16 Trikon Technologies Limited, Newport Elektrostatische Klemmhalterung für dünne Wafer in einer Vakuumkammer zur Plasmabearbeitung
US20040241748A1 (en) * 2003-02-10 2004-12-02 Dana Ault-Riche Self-assembling arrays and uses thereof
EP2194133B1 (en) 2003-03-06 2015-12-02 BASF Enzymes LLC Amylases, nucleic acids encoding them and methods for making and using them
JP4829105B2 (ja) 2003-03-07 2011-12-07 ヴェレニウム コーポレイション ヒドロラーゼ、それをコードする核酸並びにその製造および使用方法
US7083931B2 (en) 2003-04-04 2006-08-01 Stratagene California Renilla GFP mutants with increased fluorescent intensity and spectral shift
PL1613733T3 (pl) 2003-04-04 2015-11-30 Basf Enzymes Llc Liazy pektynianowe, kodujące je kwasy nukleinowe oraz sposoby ich wytwarzania i zastosowania
MXPA05010773A (es) 2003-04-09 2005-12-12 Neose Technologies Inc Metodos de glicopegilacion y proteinas/peptidos producidos por los metodos.
US7402386B2 (en) 2003-04-14 2008-07-22 Nugen Technologies, Inc. Global amplification using random priming by a composite primer
HRP20050930A2 (en) 2003-04-29 2006-03-31 E. I. du Pont de Nemours and Company Pioneer Hi-Bred International Verdia Novel glyphosate-n-acetyltransferase (gat) genes
EP1628680B1 (en) 2003-05-15 2012-10-24 Novartis Vaccines and Diagnostics, Inc. Hiv polynucleotides and polypeptides derived from botswana mj4
US7524664B2 (en) 2003-06-17 2009-04-28 California Institute Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome P450
WO2005017106A2 (en) 2003-06-17 2005-02-24 California Institute Of Technology Libraries of optimized cytochrome p450 enzymes and the optimized p450 enzymes
US20050048578A1 (en) * 2003-06-26 2005-03-03 Epitomics, Inc. Methods of screening for monoclonal antibodies with desirable activity
EP2404931A1 (en) 2003-07-02 2012-01-11 Verenium Corporation Glucanases, nucleic acids encoding them and methods for making and using them
EP1660646B1 (en) 2003-08-11 2014-12-31 California Institute Of Technology Thermostable peroxide-driven cytochrome p450 oxygenase variants and methods of use
US7741089B2 (en) 2003-08-11 2010-06-22 Verenium Corporation Laccases, nucleic acids encoding them and methods for making and using them
AU2004266199B2 (en) 2003-08-25 2010-09-16 Amyra Biotech Ag Novel fungal proteins and nucleic acids encoding same
DE602004032042D1 (de) * 2003-08-27 2011-05-12 Proterec Ltd Bibliotheken rekombinanter chimärischer proteine
US20070149496A1 (en) * 2003-10-31 2007-06-28 Jack Tuszynski Water-soluble compound
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
AU2004312215B2 (en) 2003-12-24 2009-03-05 Dupont Nutrition Biosciences Aps Proteins
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
ES2552053T3 (es) 2004-02-25 2015-11-25 Pioneer Hi-Bred International Inc. Nuevos polipéptidos cristalinos de Bacillus thuringiensis, polinucleótidos, y composiciones de los mismos
EP1718742B1 (en) 2004-02-25 2014-12-24 Novozymes A/S Fungal cell wall degrading enzyme
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
US7622125B2 (en) * 2004-05-05 2009-11-24 Novartis Vaccines And Diagnostics, Inc. Polycistronic HIV vector constructs
EP3985125A1 (en) * 2004-06-01 2022-04-20 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification
AU2015203225B2 (en) * 2004-06-01 2017-05-04 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification
CA2734561A1 (en) 2004-06-09 2005-12-29 Pioneer Hi-Bred International, Inc. Plastid transit peptides
WO2005123956A2 (en) * 2004-06-09 2005-12-29 Wisconsin Alumni Research Foundation Rapid production of oligonucleotides
EA013993B1 (ru) 2004-06-16 2010-08-30 Верениум Корпорейшн Композиции для ферментативного обесцвечивания хлорофилла и способы
PL1776455T3 (pl) 2004-07-16 2015-09-30 Dupont Nutrition Biosci Aps Enzym lipolityczny, jego zastosowania w przemyśle spożywczym
GB0421598D0 (en) 2004-09-29 2004-10-27 Cambridge Advanced Tech Modification of plant development and morphology
CA2584984A1 (en) * 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US20060204979A1 (en) * 2004-12-01 2006-09-14 Invitrogen Corporation Reverse two-hybrid system for identification of interaction domains
DE102005005438A1 (de) * 2005-02-05 2006-08-24 Bayer Healthcare Ag Mutanten des fluoreszierenden Proteins CGFPs, sowie deren Verwendung
EP1856156A2 (en) 2005-02-08 2007-11-21 ZymoGenetics, Inc. Anti-il-20, anti-il-22 and anti-il-22ra antibodies and binding partners and methods of using in inflammation
CA2614769A1 (en) 2005-03-10 2006-09-21 Verenium Corporation Lyase enzymes, nucleic acids encoding them and methods for making and using them
CA2611859C (en) 2005-03-15 2015-03-31 Verenium Corporation Cellulases, nucleic acids encoding them and methods for making and using them
US11214817B2 (en) 2005-03-28 2022-01-04 California Institute Of Technology Alkane oxidation by modified hydroxylases
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
US20070009928A1 (en) * 2005-03-31 2007-01-11 Lathrop Richard H Gene synthesis using pooled DNA
US8076108B2 (en) * 2005-04-26 2011-12-13 Cargill, Incorporated Polypeptides and biosynthetic pathways for the production of stereoisomers of monatin and their precursors
ES2609429T3 (es) 2005-05-12 2017-04-20 Zymogenetics, Inc. Composiciones y métodos para modular respuestas inmunitarias
MX2007016032A (es) 2005-06-21 2008-03-10 Xoma Technology Ltd Anticuerpos de enlace a il-1-beta y fragmentos de los mismos.
EP2385140B1 (en) 2005-07-25 2014-07-16 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
US7939258B2 (en) * 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
AU2006318652A1 (en) * 2005-11-18 2007-05-31 Gloucester Pharmaceuticals, Inc. Metabolite derivatives of the HDAC inhibitor FK228
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
EP1973937B1 (en) * 2006-01-11 2011-05-25 Axxam S.P.A. Luminescent transgenic non human animals, progeny, cell derivatives and uses thereof
WO2007092314A2 (en) 2006-02-02 2007-08-16 Verenium Corporation Esterases and related nucleic acids and methods
CA2637013A1 (en) 2006-02-10 2007-08-23 Verenium Corporation Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
BRPI0707784B1 (pt) 2006-02-14 2018-05-22 Verenium Corporation Ácido nucléico isolado, sintético ou recombinante, cassete de expressão, vetor ou veículo de clonagem, célula hospedeira isolada transformada, e método para produção de um polipeptídeo recombinante
ES2383767T3 (es) 2006-03-07 2012-06-26 Verenium Corporation Aldolasas, ácidos nucleicos que las codifican y métodos para producir y usar las mismas
WO2007103389A2 (en) 2006-03-07 2007-09-13 Cargill, Incorporated Aldolases, nucleic acids encoding them and methods for making and using them
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
CA2649877A1 (en) * 2006-04-24 2007-12-21 Gloucester Pharmaceuticals Gemcitabine combination therapy
JP2009535053A (ja) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック， インコーポレイテッド レコンビナーゼポリメラーゼ増幅
WO2007137163A2 (en) 2006-05-19 2007-11-29 Georgia Tech Research Corporation Abc transporter ligand
WO2007146730A2 (en) 2006-06-08 2007-12-21 Gloucester Pharmaceuticals Deacetylase inhibitor therapy
US7572618B2 (en) 2006-06-30 2009-08-11 Bristol-Myers Squibb Company Polynucleotides encoding novel PCSK9 variants
WO2008008873A2 (en) 2006-07-14 2008-01-17 Georgia Tech Research Corporation Clc channel ligand
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8026085B2 (en) 2006-08-04 2011-09-27 California Institute Of Technology Methods and systems for selective fluorination of organic molecules
EP2444413A1 (en) 2006-08-04 2012-04-25 Verenium Corporation Methods for oil or gas well drilling, washing and/or fracturing
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
MX2009003034A (es) 2006-09-21 2009-11-18 Verenium Corp Fosfolipasas, acidos nucleicos que las codifican, y metodos de hacerlas y usarlas.
EP2617822A3 (en) 2006-09-21 2013-10-16 Verenium Corporation Phytases, nucleic acids encoding them and methods for making and using them
CN101578370B (zh) 2006-12-20 2015-11-25 孟山都巴西有限公司 编码c3hc4家族植物蛋白质的核酸分子和改变植物纤维素和木素含量的方法
BRPI0720468A2 (pt) 2006-12-20 2014-01-14 Alellyx Sa Construtos de ácido nucleico e métodos para alterar o comprimento da fibra da planta e/ou a altura da planta
ES2531167T5 (es) 2006-12-21 2018-05-21 Basf Enzymes Llc Amilasas y glucoamilasas, ácidos nucleicos que las codifican y métodos para formarlas y utilizarlas
BRPI0720991A8 (pt) * 2006-12-29 2016-03-15 Gloucester Pharmaceuticals Inc Preparação da romidepsina
AU2007342030B2 (en) * 2006-12-29 2013-08-15 Celgene Corporation Romidepsin-based treatments for cancer
DE102007003143A1 (de) 2007-01-16 2008-07-17 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
AU2007344910B2 (en) 2007-01-25 2013-03-28 Dupont Nutrition Biosciences Aps Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
NZ610301A (en) 2007-01-30 2015-03-27 Bp Corp North America Inc Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
WO2009008908A2 (en) 2007-02-12 2009-01-15 Codexis, Inc. Structure-activity relationships
EP2124988B1 (en) 2007-02-23 2014-11-12 The Trustees of Columbia University in the City of New York Methods to activate or block the hla-e/qa-1 restricted cd8+ t cell regulatory pathway to treat immunological disease
US7883879B2 (en) 2007-10-01 2011-02-08 Codexis, Inc. Ketoreductase polypeptides for the production of azetidinone
US8486680B2 (en) 2007-10-03 2013-07-16 Bp Corporation North America Inc. Xylanases, nucleic acids encoding them and methods for making and using them
AU2008308509B2 (en) 2007-10-04 2014-10-23 Zymogenetics, Inc. B7 family member zB7H6 and related compositions and methods
DE102007051092A1 (de) 2007-10-24 2009-04-30 Henkel Ag & Co. Kgaa Subtilisin aus Becillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin
CN101998965B (zh) 2007-11-01 2014-03-12 安斯泰来制药有限公司 免疫抑制性多肽与核酸
WO2009059379A1 (en) 2007-11-07 2009-05-14 Dynamic Microbials Limited Antimicrobial compositions, formulations and uses thereof
MX2010005565A (es) 2007-11-20 2010-06-11 Pioneer Hi Bred Int Genes de señalizacion de etileno en maiz y su modulacion para mejor tolerancia al estres en plantas.
WO2009065415A1 (en) 2007-11-21 2009-05-28 Roskilde Universitet Polypeptides comprising an ice-binding activity
WO2009065934A1 (en) * 2007-11-22 2009-05-28 Novozymes A/S Polypeptides having ferulic acid esterase activity and polynucleotides encoding same
NZ585870A (en) 2007-12-21 2012-04-27 Danisco Process for edible oil refining using a lipid acyltransferase
CA2710922A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Isomerases, nucleic acids encoding them and methods for making and using them
US8709772B2 (en) 2008-01-03 2014-04-29 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
BRPI0907640A2 (pt) 2008-01-25 2015-11-03 Univ Aarhus inibição exosítio-seletiva da atividade de papp-a contra igfbp-4
WO2009102901A1 (en) 2008-02-12 2009-08-20 Codexis, Inc. Method of generating an optimized, diverse population of variants
US8034568B2 (en) * 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US8504498B2 (en) 2008-02-12 2013-08-06 Codexis, Inc. Method of generating an optimized, diverse population of variants
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
CN102066409A (zh) 2008-04-17 2011-05-18 诺维信公司 具有阿魏酸酯酶活性的多肽和编码该多肽的多核苷酸
GB0807161D0 (en) * 2008-04-18 2008-05-21 Danisco Process
CA2990650C (en) 2008-05-23 2021-02-09 Pioneer Hi-Bred International, Inc. Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
CN102131813B (zh) 2008-06-24 2014-07-30 科德克希思公司 用于制备基本上立体异构纯的稠合二环脯氨酸化合物的生物催化方法
US8426178B2 (en) * 2008-08-27 2013-04-23 Codexis, Inc. Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
US8288141B2 (en) * 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
WO2010027710A2 (en) 2008-08-27 2010-03-11 Codexis, Inc. Ketoreductase polypeptides and uses thereof
WO2010025085A2 (en) 2008-08-29 2010-03-04 Codexis, Inc. Ketoreductase polypeptides for the stereoselective production of (4s)-3[(5s)-5(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
US8153391B2 (en) 2008-08-29 2012-04-10 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8357503B2 (en) 2008-08-29 2013-01-22 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US20100081575A1 (en) * 2008-09-22 2010-04-01 Robert Anthony Williamson Methods for creating diversity in libraries and libraries, display vectors and methods, and displayed molecules
DK2346995T3 (en) 2008-09-26 2019-02-11 Tocagen Inc GENETERIVE VECTORS AND CYTOSINDEAMINASES
AU2009313560B2 (en) 2008-11-07 2016-04-14 Fabrus Llc Combinatorial antibody libraries and uses thereof
US8247192B2 (en) * 2008-11-10 2012-08-21 Codexis, Inc. Penicillin-G acylases
JP5645840B2 (ja) 2008-12-02 2014-12-24 株式会社Ｗａｖｅ Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ Ｊａｐａｎ リン原子修飾核酸の合成方法
EP2789618B1 (en) 2008-12-03 2016-07-27 The Scripps Research Institute Stem cell cultures
WO2010065867A1 (en) 2008-12-04 2010-06-10 Pioneer Hi-Bred International, Inc. Methods and compositions for enhanced yield by targeted expression of knotted1
US8187856B2 (en) * 2008-12-18 2012-05-29 Codexis, Inc. Recombinant halohydrin dehalogenase polypeptides
SG172783A1 (en) 2008-12-25 2011-08-29 Codexis Inc Enone reductases
US20120034650A1 (en) 2009-02-13 2012-02-09 Novartis Ag Nucleic acid molecule of a biosynthetic cluster encoding non ribosomal peptide synthases and uses thereof
US8715996B2 (en) 2009-02-26 2014-05-06 Codexis, Inc. Beta-glucosidase variant enzymes and related polynucleotides
WO2010099501A2 (en) 2009-02-26 2010-09-02 Codexis, Inc. Transaminase biocatalysts
WO2010101818A1 (en) 2009-03-02 2010-09-10 Pioneer Hi-Bred International, Inc. Nac transcriptional activators involved in abiotic stress tolerance
EP2408908B1 (en) 2009-03-17 2015-05-27 Codexis, Inc. Variant endoglucanases and related polynucleotides
MX2011010097A (es) 2009-03-27 2011-10-19 Proyecto Biomedicina Cima Sl Metodos y composiciones para el tratamiento de cirrosis y fibrosis hepatica.
WO2010120557A1 (en) 2009-03-31 2010-10-21 Codexis, Inc. Improved endoglucanases
US20120058222A1 (en) 2009-05-19 2012-03-08 Danisco A/S Use
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
CA2763283C (en) 2009-05-21 2021-01-12 Verenium Corporation Phytases, nucleic acids encoding them and methods for making and using them
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
AR077042A1 (es) 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina
CA2765715A1 (en) 2009-06-16 2010-12-23 Codexis, Inc. .beta.-glucosidase variants
CN102597226B (zh) 2009-06-22 2015-08-19 科德克希思公司 转氨酶反应
DK3409765T3 (da) 2009-06-22 2021-09-27 Codexis Inc Ketoreduktase-medieret stereoselektiv rute til alpha-chloralkoholer
IN2012DN00720A (enExample) 2009-07-06 2015-06-19 Ontorii Inc
US8614081B2 (en) 2009-07-23 2013-12-24 Codexis, Inc. Nitrilase biocatalysts
WO2011017492A2 (en) 2009-08-05 2011-02-10 Pioneer Hi-Bred International, Inc. Novel eto1 genes and use of same for reduced ethylene and improved stress tolerance in plants
SG10201405022PA (en) 2009-08-19 2014-10-30 Codexis Inc Ketoreductase polypeptides for the preparation of phenylephrine
EP2467395A1 (en) 2009-08-20 2012-06-27 Pioneer Hi-Bred International Inc. Functional expression of shuffled yeast nitrate transporter (ynti) in maize to improve nitrate uptake under low nitrate environment
US8785170B2 (en) 2009-09-04 2014-07-22 Codexis, Inc. Variant CBH2 cellulases and related polynucleotides
WO2011041594A1 (en) 2009-09-30 2011-04-07 Codexis, Inc. Recombinant c1 b-glucosidase for production of sugars from cellulosic biomass
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
CA2780974C (en) 2009-11-20 2016-02-02 Codexis, Inc. Mutli-cellulase enzyme compositions for hydrolysis of cellulosic biomass
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
EP2706119B1 (en) 2009-11-25 2015-08-12 Codexis, Inc. Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass
EP2510089B1 (en) 2009-12-08 2015-10-21 Codexis, Inc. Synthesis of prazole compounds
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
WO2011100265A2 (en) 2010-02-10 2011-08-18 Codexis, Inc. Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system
PL2545150T3 (pl) 2010-03-12 2019-01-31 Dupont Nutrition Biosciences Aps Sposób
WO2011114251A1 (en) 2010-03-18 2011-09-22 Danisco A/S Foodstuff
WO2011123567A1 (en) 2010-03-31 2011-10-06 Codexis, Inc. Production of geranyl diphosphate
WO2011140219A1 (en) 2010-05-04 2011-11-10 Codexis, Inc. Biocatalysts for ezetimibe synthesis
EP2569426A4 (en) 2010-05-14 2013-10-09 Codexis Inc ZELLBIOHYDROLASE VARIANTS
CA2798937A1 (en) 2010-05-28 2011-12-01 Codexis, Inc. Pentose fermentation by a recombinant microorganism
EP2580238A1 (en) 2010-06-09 2013-04-17 Zymogenetics, Inc. Dimeric vstm3 fusion proteins and related compositions and methods
EP2582809A1 (en) 2010-06-17 2013-04-24 DuPont Nutrition Biosciences ApS Process
SG10201505475XA (en) 2010-07-12 2015-09-29 Celgene Corp Romidepsin solid forms and uses thereof
WO2012021794A1 (en) 2010-08-13 2012-02-16 Pioneer Hi-Bred International, Inc. Chimeric promoters and methods of use
EP2606139B1 (en) 2010-08-16 2015-07-15 Codexis, Inc. Biocatalysts and methods for the synthesis of (1r,2r)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine
BR112013003906A2 (pt) 2010-08-20 2018-07-17 Codexis Inc uso de proteínas da família glicosideo hidrolase no processamento de celulose.
US9322027B2 (en) 2010-08-20 2016-04-26 Shell Oil Company Expression constructs comprising fungal promoters
US8859502B2 (en) 2010-09-13 2014-10-14 Celgene Corporation Therapy for MLL-rearranged leukemia
US10428019B2 (en) 2010-09-24 2019-10-01 Wave Life Sciences Ltd. Chiral auxiliaries
EP2625269A2 (en) 2010-10-06 2013-08-14 BP Corporation North America Inc. Variant cbh i polypeptides with reduced product inhibition
US20130344541A1 (en) 2010-11-02 2013-12-26 Codexis, Inc. Compositions and methods for production of fermentable sugars
US9068235B2 (en) 2010-11-02 2015-06-30 Codexis, Inc. Fungal strains
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
WO2012078800A2 (en) 2010-12-08 2012-06-14 Codexis, Inc. Biocatalysts and methods for the synthesis of armodafinil
US8906689B2 (en) 2010-12-21 2014-12-09 Codexis, Inc. Endoglucanase variants
WO2012106579A1 (en) 2011-02-03 2012-08-09 Bristol-Myers Squibb Company Amino acid dehydrogenase and its use in preparing amino acids from keto acids
CA2825628A1 (en) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Method
US9493749B2 (en) 2011-02-23 2016-11-15 Dupont Nutrition Biosciences Aps Process modifications to enhance chlorophyll degradation
AR085251A1 (es) 2011-02-23 2013-09-18 Danisco Proceso para tratar aceite vegetal
EP2691517A4 (en) 2011-03-30 2015-04-22 Codexis Inc FERMENTATION OF PENTOSES BY A RECOMBINANT MICROORGANISM
ES2905686T3 (es) 2011-04-07 2022-04-11 Abbott Diagnostics Scarborough Inc Control de mezclas de amplificación de recombinasa polimerasa
CA2830579A1 (en) 2011-04-08 2012-10-11 Danisco Us Inc. Compositions
WO2012142302A2 (en) 2011-04-13 2012-10-18 Codexis, Inc. Biocatalytic process for preparing eslicarbazepine and analogs thereof
BR112013033614A2 (pt) 2011-06-30 2018-05-29 Codexis Inc fermentação de pentose por microorganismo recombinante
RU2014105311A (ru) 2011-07-19 2015-08-27 Уэйв Лайф Сайенсес Пте. Лтд. Способы синтеза функционализованных нуклеиновых кислот
US9322007B2 (en) 2011-07-22 2016-04-26 The California Institute Of Technology Stable fungal Cel6 enzyme variants
EP2748317B1 (en) 2011-08-22 2017-04-19 Codexis, Inc. Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity
EP2748187A4 (en) 2011-08-23 2015-03-04 Codexis Inc VARIANTS OF CELLOBIOHYDROLASE
EP3594340B1 (en) 2011-08-26 2021-06-30 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US9109209B2 (en) 2011-09-08 2015-08-18 Codexis, Inc. Biocatalysts and methods for the synthesis of substituted lactams
EP2760997A4 (en) 2011-09-30 2015-02-11 Codexis Inc MUSHROOM PROTEASE
CN103906835A (zh) 2011-10-25 2014-07-02 先锋国际良种公司 改变植物细胞壁组成以改善生物燃料生产和青贮饲料可消化性的方法
CN109897103B (zh) 2011-11-04 2024-05-17 酵活英属哥伦比亚有限公司 在Fc结构域中具有突变的稳定异源二聚的抗体设计
CN104053771B (zh) 2011-11-18 2016-11-09 科德克希思公司 用于制备羟基取代的氨基甲酸酯的生物催化剂
WO2013096082A1 (en) 2011-12-20 2013-06-27 Codexis, Inc. Fatty alcohol forming acyl reductase (far) variants and methods of use
US9611462B2 (en) 2011-12-20 2017-04-04 Codexis, Inc. Endoglucanase 1B (EG1B) variants
WO2013104659A2 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process
WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
EP2825647A4 (en) * 2012-03-15 2015-10-14 Codexis Inc METHODS OF GENE REARRANGING
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
SG11201405990PA (en) 2012-03-23 2014-11-27 Codexis Inc Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs
US20150133698A1 (en) 2012-04-20 2015-05-14 Codexis, Inc. Production of fatty alcohols from engineered microorganisms
EP3543350B1 (en) 2012-04-24 2021-11-10 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2013160372A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
WO2013160374A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling
RU2014148769A (ru) 2012-05-04 2016-06-27 Е.И. Дюпон Де Немур Энд Компани Композиции и способы, включающие последовательности, характеризующиеся мегануклеазной активностью
SG11201407295YA (en) 2012-05-08 2014-12-30 Codexis Inc Biocatalysts and methods for hydroxylation of chemical compounds
WO2013170050A1 (en) 2012-05-11 2013-11-14 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
US10045499B2 (en) 2012-05-24 2018-08-14 Iowa State University Research Foundation, Inc. Arabidopsis nonhost resistance gene(s) and use thereof to engineer disease resistant plants
US8980578B2 (en) 2012-06-11 2015-03-17 Codexis, Inc. Fungal beta-xylosidase variants
CA2877823A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
SG11201500232UA (en) 2012-07-13 2015-04-29 Wave Life Sciences Pte Ltd Chiral control
SG11201500239VA (en) 2012-07-13 2015-03-30 Wave Life Sciences Japan Asymmetric auxiliary group
CA2879066C (en) 2012-07-13 2019-08-13 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant
WO2014015278A1 (en) 2012-07-20 2014-01-23 Codexis, Inc. Production of fatty alcohols from engineered microorganisms
US9506087B2 (en) 2012-08-07 2016-11-29 Codexis, Inc. Glucose and xylose co-utilization in E. coli
AU2013302535B2 (en) 2012-08-16 2018-12-06 Bangladesh Jute Research Institute Pectin degrading enzymes from Macrophomina phaseolina and uses thereof
PT2885406T (pt) 2012-08-16 2018-11-16 Bangladesh Jute Res Institute Enzimas degradantes de celulose e/ou hemiceluloses de macrophomina phaseolina e utilizações das mesmas
AU2013202506B2 (en) 2012-09-07 2015-06-18 Celgene Corporation Resistance biomarkers for hdac inhibitors
WO2014063097A1 (en) 2012-10-19 2014-04-24 Danisco Us Inc. Stabilization of biomimetic membranes
US9663532B2 (en) 2012-10-29 2017-05-30 University Of Rochester Artemisinin derivatives, methods for their preparation and their use as antimalarial agents
AU2013202507B9 (en) 2012-11-14 2015-08-13 Celgene Corporation Inhibition of drug resistant cancer cells
BR112015011466A8 (pt) 2012-11-20 2018-05-02 Codexis Inc Célula hospedeira fúngica recombinante, constructo de ácido nucleico recombinante, sequência de polpeptídeo isolada, e, métodos para produzir um produto de fermentação e pelo menos um produto final a partir de pelo menos um substrato celulósico
CN104837994A (zh) * 2012-12-06 2015-08-12 安捷伦科技有限公司 分子制造
WO2014093070A1 (en) 2012-12-14 2014-06-19 Codexis, Inc. Modified native beta-ketoacyl-acp synthases and engineered microorganisms
CN120829884A (zh) 2012-12-21 2025-10-24 科德克希思公司 用于合成手性胺的工程化的生物催化剂和方法
EP2946006B1 (en) 2013-01-18 2019-04-10 Codexis, Inc. Engineered biocatalysts useful for carbapenem synthesis
KR20150113166A (ko) 2013-01-31 2015-10-07 코덱시스, 인코포레이티드 상호작용 성분을 이용하여 생체분자를 확인하기 위한 방법, 시스템, 및 소프트웨어
US20140288097A1 (en) 2013-02-07 2014-09-25 The Regents Of The University Of California Use of translational profiling to identify target molecules for therapeutic treatment
SI2961844T1 (sl) 2013-02-28 2018-11-30 Codexis, Inc. Spremenjeni polipeptidi transaminaze za industrijsko biokatalizo
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
US20140289906A1 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International, Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
WO2014153242A1 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
EP2970869A4 (en) 2013-03-14 2016-08-03 Codexis Inc REPRESSIBLE PROMOTER WITH LOW PHOSPHATE CONTENT
EP2970361B1 (en) * 2013-03-15 2023-02-15 Gen9, Inc. Compositions and methods for multiplex nucleic acids synthesis
EP2971000A4 (en) 2013-03-15 2016-11-23 Pioneer Hi Bred Int PHI-4 POLYPEPTIDES AND METHOD FOR THEIR USE
US20160024516A1 (en) 2013-03-15 2016-01-28 Pioneer Hi-Bred International, Inc. Modulation of ACC Deaminase Expression
DK2986722T3 (da) 2013-04-18 2019-05-13 Codexis Inc Manipulerede phenylalaninammoniak-lyasepolypeptider
WO2014202089A2 (en) 2013-06-18 2014-12-24 Roskilde Universitet Variants of anti-freeze polypeptides
KR20160033711A (ko) 2013-07-25 2016-03-28 바스프 엔자임스 엘엘씨 피타아제
CA2920339C (en) 2013-08-16 2023-10-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3223359A1 (en) 2013-09-13 2015-03-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
DK3049973T3 (en) 2013-09-27 2018-10-22 Codexis Inc AUTOMATED SCREENING OF ENZYM VARIENTS
US9822346B2 (en) 2013-11-13 2017-11-21 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
EP3083936B1 (en) 2013-12-19 2018-07-04 Danisco US Inc. Use of hydrophobins to increase gas transfer in aerobic fermentation processes
WO2015100277A2 (en) 2013-12-23 2015-07-02 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
NZ630311A (en) 2013-12-27 2016-03-31 Celgene Corp Romidepsin formulations and uses thereof
JPWO2015108047A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤
US10149905B2 (en) 2014-01-15 2018-12-11 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having antitumor effect and antitumor agent
WO2015108046A1 (ja) 2014-01-15 2015-07-23 株式会社新日本科学 抗アレルギー作用を有するキラル核酸アジュバンド及び抗アレルギー剤
KR102423317B1 (ko) 2014-01-16 2022-07-22 웨이브 라이프 사이언시스 리미티드 키랄 디자인
US10227608B2 (en) 2014-02-07 2019-03-12 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
EP3102684B1 (en) 2014-02-07 2020-05-06 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3126509A1 (en) 2014-04-01 2017-02-08 DuPont Nutrition Biosciences ApS Method for increasing crude palm oil yields
CA2943432A1 (en) 2014-04-16 2015-10-22 Codexis, Inc. Engineered tyrosine ammonia lyase
US9708587B2 (en) 2014-07-09 2017-07-18 Codexis, Inc. P450-BM3 variants with improved activity
EP3778891A1 (en) 2014-08-27 2021-02-17 New England Biolabs, Inc. Synthon formation
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
WO2016033296A1 (en) 2014-08-28 2016-03-03 Codexis, Inc. N-substituted 4-aminoquinazoline derivatives and methods of use
US10045987B2 (en) 2014-08-28 2018-08-14 Codexis, Inc. Imidazoyl anilide derivatives and methods of use
MX381370B (es) 2014-10-16 2025-03-12 Corteva Agriscience Llc Proteinas insecticidas y metodos de uso.
EP3224354B1 (en) 2014-11-25 2021-01-13 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
WO2016183532A1 (en) 2015-05-13 2016-11-17 Synlogic, Inc. Bacteria engineered to treat a disease or disorder
AU2015370125B2 (en) 2014-12-22 2022-02-03 Codexis, Inc. Human alpha-galactosidase variants
AU2016207026B2 (en) 2015-01-15 2021-12-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3256577B1 (en) 2015-02-10 2020-08-26 Codexis, Inc. Ketoreductase polypeptides for the synthesis of chiral compounds
CN107529763B (zh) 2015-03-11 2021-08-20 先锋国际良种公司 Pip-72的杀昆虫组合及使用方法
EP3280817A2 (en) 2015-04-07 2018-02-14 Novozymes A/S Methods for selecting enzymes having enhanced activity
CN107864658A (zh) 2015-04-07 2018-03-30 诺维信公司 用于选择具有脂肪酶活性的酶的方法
AU2016258091B2 (en) 2015-05-07 2020-10-01 Codexis, Inc. Penicillin-G acylases
US9920102B2 (en) 2015-05-15 2018-03-20 Albert Einstein College Of Medicine, Inc. Fusion tags for protein expression
CA2985198A1 (en) 2015-05-19 2016-11-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
JP6867302B2 (ja) * 2015-05-21 2021-04-28 フル スペクトラム ジェネティクス， インコーポレイテッド タンパク質の特徴を改善する方法
WO2016210384A2 (en) 2015-06-25 2016-12-29 Synlogic, Inc. Bacteria engineered to treat metabolic diseases
WO2017007547A1 (en) 2015-07-07 2017-01-12 Codexis, Inc. Novel p450-bm3 variants with improved activity
CN109475096B (zh) 2015-08-06 2022-08-23 先锋国际良种公司 植物来源的杀昆虫蛋白及其使用方法
CA2997263C (en) 2015-09-08 2022-10-04 Theripion, Inc. Apoa-1 fusion polypeptides and related compositions and methods
GB201516348D0 (en) * 2015-09-15 2015-10-28 Labgenius Ltd Compositions and methods for polynucleotide assembly
JP6821598B2 (ja) 2015-12-07 2021-01-27 ザイマージェン インコーポレイテッド Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍ ｇｌｕｔａｍｉｃｕｍ由来のプロモーター
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US20180325119A1 (en) 2015-12-18 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2017123592A1 (en) 2016-01-11 2017-07-20 Synlogic, Inc. Bacteria engineered to treat disorders associated with bile salts
WO2017123676A1 (en) 2016-01-11 2017-07-20 Synlogic, Inc. Recombinant bacteria engineered to treat diseases and disorders associated with amino acid metabolism and methods of use thereof
EP3402497A1 (en) 2016-01-11 2018-11-21 Synlogic Operating Company, Inc. Bacteria engineered to treat metabolic diseases
WO2017139708A1 (en) 2016-02-10 2017-08-17 Synlogic, Inc. Bacteria engineered to treat nonalcoholic steatohepatitis (nash)
EP3440207A1 (en) 2016-04-07 2019-02-13 Novozymes A/S Methods for selecting enzymes having protease activity
EA201892293A1 (ru) 2016-05-04 2019-04-30 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
WO2017193022A1 (en) 2016-05-05 2017-11-09 Codexis, Inc. Penicillin-g acylases
CN109715817B (zh) 2016-06-09 2022-12-09 科德克希思公司 用于化合物的羟基化的生物催化剂和方法
WO2017218325A1 (en) 2016-06-15 2017-12-21 Codexis, Inc. Engineered beta-glucosidases and glucosylation methods
US20190330641A1 (en) 2016-06-17 2019-10-31 National Hellenic Research Foundation Systems for recombinant protein production
US10544411B2 (en) 2016-06-30 2020-01-28 Zymergen Inc. Methods for generating a glucose permease library and uses thereof
US10544390B2 (en) 2016-06-30 2020-01-28 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
EP3954202A1 (en) 2016-07-01 2022-02-16 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
JP7316662B2 (ja) 2016-08-26 2023-07-28 コデクシス， インコーポレイテッド 操作されたイミンレダクターゼ、ならびにケトン化合物およびアミン化合物の還元的アミノ化のための方法
MX387927B (es) 2016-11-01 2025-03-19 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
WO2018136163A2 (en) 2016-12-09 2018-07-26 Theripion, Inc. Tandem apoa-1 fusion polypeptides
US11174295B2 (en) 2016-12-14 2021-11-16 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3558004A1 (en) 2016-12-22 2019-10-30 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3048475A1 (en) 2017-01-05 2018-07-12 Codexis, Inc. Penicillin-g acylases
EP3882354A3 (en) 2017-02-03 2021-12-15 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
MX2019009371A (es) 2017-02-08 2019-09-23 Pionner Hi Bred Int Inc Combinaciones insecticidas de proteinas insecticidas derivadas de plantas y metodos para su uso.
BR112019016634A2 (pt) 2017-02-13 2020-04-07 Codexis Inc polipeptídeos, sequência de polinucleotídeos modificados por engenharia, vetor de expressão, célula hospedeira, métodos para produzir um polipeptídeo de fenilalanina amônia liase modificado por engenharia em uma célula hospedeira e para tratar e/ou prevenir os sintomas de fenilcetonúria em um indivíduo, composição, e, uso
WO2018200214A2 (en) 2017-04-27 2018-11-01 Codexis, Inc. Ketoreductase polypeptides and polynucleotides
WO2018208665A1 (en) 2017-05-08 2018-11-15 Codexis, Inc. Engineered ligase variants
WO2018209301A1 (en) 2017-05-11 2018-11-15 Cytodyn Inc. Methods for treating or preventing graft-versus-host disease involving the administration of anti-ccr5 receptor agents
WO2018208882A1 (en) 2017-05-11 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2018226880A1 (en) * 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving escherichia coli
JP7350659B2 (ja) * 2017-06-06 2023-09-26 ザイマージェン インコーポレイテッド Ｓａｃｃｈａｒｏｐｏｌｙｓｐｏｒａ ｓｐｉｎｏｓａの改良のためのハイスループット（ＨＴＰ）ゲノム操作プラットフォーム
WO2018231462A1 (en) 2017-06-14 2018-12-20 Codexis, Inc. Engineered transaminase polypeptides for industrial biocatalysis
US11643642B2 (en) 2017-06-27 2023-05-09 Codexis, Inc. Penicillin-g acylases
US10793841B2 (en) 2017-06-30 2020-10-06 Codexis, Inc. T7 RNA polymerase variants
JP2020530267A (ja) 2017-06-30 2020-10-22 コデクシス， インコーポレイテッド Ｔ７ ｒｎａポリメラーゼバリアント
WO2019040335A1 (en) 2017-08-19 2019-02-28 University Of Rochester MICHELOLID DERIVATIVES, PREPARATION METHODS AND THEIR USE AS ANTICANCER AND ANTI-INFLAMMATORY AGENTS
US11319531B2 (en) 2017-11-07 2022-05-03 Codexis, Inc. Transglutaminase variants
SG11202004185PA (en) 2017-12-13 2020-06-29 Codexis Inc Carboxyesterase polypeptides for amide coupling
AU2019234566B2 (en) 2018-03-14 2024-09-26 Hexima Limited Insecticidal proteins from plants and methods for their use
EP3764796B1 (en) 2018-03-14 2025-12-03 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
WO2019222226A2 (en) 2018-05-17 2019-11-21 Bp Corporation North America Inc. Production of 2-keto-3-deoxy-d-gluconic acid in filamentous fungi
EP3807409A4 (en) 2018-06-12 2022-08-03 Codexis, Inc. Engineered tyrosine ammonia lyase
WO2020014046A1 (en) 2018-07-09 2020-01-16 Codexis, Inc. Engineered phosphopentomutase variant enzymes
KR20210031926A (ko) 2018-07-09 2021-03-23 코덱시스, 인코포레이티드 조작된 퓨린 뉴클레오사이드 포스포릴라제 변이체 효소
MX2021000328A (es) 2018-07-09 2021-03-25 Codexis Inc Enzimas de variantes de galactosa oxidasa modificadas geneticamente.
CA3103819A1 (en) 2018-07-09 2020-01-16 Codexis, Inc. Engineered pantothenate kinase variant enzymes
SG11202012107RA (en) 2018-07-09 2021-01-28 Codexis Inc Engineered deoxyribose-phosphate aldolases
EP3820833A4 (en) 2018-07-12 2022-08-03 Codexis, Inc. MODIFIED AMMONIA-LYASE PHENYLALANINE POLYPEPTIDES
BR112021001661A8 (pt) 2018-07-30 2022-08-09 Codexis Inc Glicosiltransferase modificada, polinucleotídeo modificado, vetor, célula hospedeira, métodos para produzir pelo menos uma glicosiltransferase modificada, para produzir pelo menos uma variante de sacarose sintase, para glicosilação de um substrato, para produzir rebaudiosídeo m, rebaudiosídeo a e/ou rebaudiosídeo i e rebaudiosídeo d, composição, sacarose sintase modificada, pelo menos um rebaudiosídeo, rebaudiosídeo m, rebaudiosídeo a, rebaudiosídeo i, e, rebaudiosídeo d.
CN112969792A (zh) 2018-09-07 2021-06-15 巴斯夫植物科学有限公司 用于在植物中产生高水平pufa的改进方法
EP3847264A1 (en) 2018-09-07 2021-07-14 Basf Plant Science Company GmbH Improved method for the production of high levels of pufa in plants
AU2019334671A1 (en) 2018-09-07 2021-03-25 Basf Plant Science Company Gmbh Improved method for the production of high levels of PUFA in plants
SG11202103639SA (en) 2018-10-29 2021-05-28 Codexis Inc Engineered dna polymerase variants
CA3122524A1 (en) 2018-12-14 2020-06-18 Codexis, Inc. Engineered tyrosine ammonia lyase
CA3123598A1 (en) 2018-12-20 2020-06-25 Codexis, Inc. Human alpha-galactosidase variants
EP3906312A4 (en) 2019-01-02 2022-09-28 Intima Bioscience, Inc. MODIFIED ADENO-ASSOCIATED VIRAL VECTORS FOR USE IN GENETIC ENGINEERING
WO2020150125A1 (en) 2019-01-15 2020-07-23 Codexis, Inc. Engineered transaminase polypeptides
CN113874392B (zh) 2019-03-28 2025-10-21 丹尼斯科美国公司 工程化抗体
CN114096662B (zh) 2019-05-01 2025-04-15 科德克希思公司 工程化葡萄糖脱氢酶和用于酮和胺化合物的还原胺化的方法
US12215368B2 (en) 2019-05-01 2025-02-04 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
AU2020299358A1 (en) 2019-07-02 2021-12-23 Codexis, Inc. Engineered acetate kinase variant enzymes
CA3142636A1 (en) 2019-07-02 2021-01-07 Codexis, Inc. Engineered sucrose phosphorylase variant enzymes
BR112022001037A2 (pt) 2019-08-02 2022-06-07 Cytodyn Inc Métodos para tratamento ou prevenção de cânceres envolvendo a administração de agentes receptores anti-ccr5
AU2020336468A1 (en) 2019-08-30 2022-03-03 Societe Des Produits Nestle S.A. Engineered lipase variants
WO2021050348A1 (en) 2019-09-12 2021-03-18 Codexis, Inc. Peroxidase activity towards 10-acetyl-3,7-dihydroxyphenoxazine
JP7720098B2 (ja) 2019-11-26 2025-08-07 コデクシス， インコーポレイテッド 化合物のヒドロキシル化のための生体触媒および方法
EP4077679A4 (en) 2019-12-20 2024-04-24 Codexis, Inc. MODIFIED ACID ALPHA-GLUCOSIDASE VARIANTS
KR20220119408A (ko) 2019-12-20 2022-08-29 바스프 에스이 터펜의 독성 감소 및 미생물에서 생산 잠재성 증가
EP4087932A4 (en) 2020-01-10 2024-01-17 The Regents of the University of California Biosynthetic platform for the production of olivetolic avid and analogues of olivetolic acid
KR20220137057A (ko) 2020-02-04 2022-10-11 코덱시스, 인코포레이티드 조작된 류신 데카르복실라제
US11045546B1 (en) 2020-03-30 2021-06-29 Cytodyn Inc. Methods of treating coronavirus infection
JP2023521772A (ja) 2020-04-10 2023-05-25 コデクシス， インコーポレイテッド 操作されたトランスアミナーゼポリペプチド
US11499166B2 (en) 2020-04-27 2022-11-15 4D Molecular Therapeutics Inc. Adeno-associated variants, formulations and methods for pulmonary delivery
WO2021245064A1 (en) 2020-06-04 2021-12-09 Isobionics B.V. Synthetic santalene synthases
CA3186978A1 (en) 2020-07-14 2022-01-20 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3191900A1 (en) 2020-08-28 2022-03-03 Codexis, Inc. Engineered amylase variants
BR112023003606A2 (pt) 2020-08-28 2023-03-28 Codexis Inc Protease recombinante, composição, sequência polinucleotídica recombinante, vetor de expressão, célula hospedeira, métodos para produzir uma protease recombinante e para tratar e/ou prevenir os sintomas de insuficiência pancreática, e, uso
CA3201757A1 (en) 2020-12-11 2022-06-16 Mathias Schuetz Recombinant acyl activating enzyme (aae) genes for enhanced biosynthesis of cannabinoids and cannabinoid precursors
WO2022133289A2 (en) 2020-12-18 2022-06-23 Codexis, Inc. Engineered uridine phosphorylase variant enzymes
EP4015626A1 (en) 2020-12-18 2022-06-22 Isobionics B.V. Enzymes and methods for fermentative production of monoterpene esters
CA3207134A1 (en) 2021-02-19 2022-08-25 Jeffrey A. Ledbetter Dnase fusion polypeptides and related compositions and methods
EP4314262A1 (en) 2021-04-02 2024-02-07 Codexis, Inc. Engineered guanylate kinase variant enzymes
EP4314261A1 (en) 2021-04-02 2024-02-07 Codexis, Inc. Engineered acetate kinase variant enzymes
EP4314264A1 (en) 2021-04-02 2024-02-07 Codexis, Inc. Engineered cyclic gmp-amp synthase (cgas) variant enzymes
CN117120600A (zh) 2021-04-02 2023-11-24 科德克希思公司 工程化腺苷酸激酶变体酶
JP2024519906A (ja) 2021-05-21 2024-05-21 コデクシス， インコーポレイテッド 操作されたメチオニンガンマリアーゼバリアント
CA3227215A1 (en) 2021-07-30 2023-02-02 Trish Choudhary Recombinant prenyltransferase polypeptides engineered for enhanced biosynthesis of cannabinoids
WO2023012111A2 (en) 2021-08-02 2023-02-09 Basf Se Novel production of aroma compounds with ionylideneethane synthases
WO2023023621A1 (en) 2021-08-19 2023-02-23 Willow Biosciences, Inc. Recombinant olivetolic acid cyclase polypeptides engineered for enhanced biosynthesis of cannabinoids
JP2024539636A (ja) 2021-10-15 2024-10-29 コデクシス， インコーポレイテッド 操作されたｄｎａポリメラーゼバリアント
WO2023069921A1 (en) 2021-10-19 2023-04-27 Epimeron Usa, Inc. Recombinant thca synthase polypeptides engineered for enhanced biosynthesis of cannabinoids
EP4426827A2 (en) 2021-11-01 2024-09-11 Syntis Bio, Inc. Engineered leucine decarboxylases
CN114015672B (zh) * 2021-12-06 2022-05-31 江南大学 一种Pfu DNA聚合酶
EP4490202A1 (en) 2022-03-11 2025-01-15 University of Rochester Cyclopeptibodies and uses thereof
WO2023245171A1 (en) 2022-06-17 2023-12-21 Synlogic Operating Company, Inc. Bacteria engineered to treat diseases associated with bile acid metabolism and methods of use thereof
WO2023245168A1 (en) 2022-06-17 2023-12-21 Synlogic Operating Company, Inc. Bacteria engineered to treat diseases associated with bile acid metabolism and methods of use thereof
WO2023250478A1 (en) 2022-06-23 2023-12-28 Synlogic Operating Company, Inc. Recombinant bacteria engineered to treat diseases associated with methionine metabolism and methods of use thereof
WO2024040020A1 (en) 2022-08-15 2024-02-22 Absci Corporation Quantitative affinity activity specific cell enrichment
WO2024259220A1 (en) 2023-06-15 2024-12-19 Theripion, Inc. Pon3 and evolved pon1 fusion polypeptides
WO2025144700A1 (en) 2023-12-27 2025-07-03 Absci Corporation Nanobody library screening using bacterial surface display
WO2025199112A2 (en) 2024-03-19 2025-09-25 General Biological Corporation Bio-based production of succinic acid using vibrio natriegens
WO2025224232A1 (en) * 2024-04-25 2025-10-30 Danmarks Tekniske Universitet Pcr leaping and applications thereof
WO2025235589A1 (en) 2024-05-08 2025-11-13 Codexis, Inc. Dna polymerase variants

Family Cites Families (119)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994379A (en) 1983-03-09 1991-02-19 Cetus Corporation Modified signal peptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5422266A (en) * 1984-12-31 1995-06-06 University Of Georgia Research Foundation, Inc. Recombinant DNA vectors capable of expressing apoaequorin
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US6492107B1 (en) * 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
DE229046T1 (de) 1985-03-30 1987-12-17 Marc Genf/Geneve Ballivet Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren.
US4959312A (en) * 1985-05-31 1990-09-25 The University Of Tennessee Research Corporation Full spectrum mutagenesis
DE3688920D1 (de) * 1985-07-03 1993-09-30 Genencor Int Hybride Polypeptide und Verfahren zu deren Herstellung.
US5866363A (en) 1985-08-28 1999-02-02 Pieczenik; George Method and means for sorting and identifying biological information
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
DK311186D0 (da) * 1986-06-30 1986-06-30 Novo Industri As Enzymer
US5824469A (en) 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
FR2641793B1 (fr) 1988-12-26 1993-10-01 Setratech Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases
DD282028A5 (de) 1989-02-16 1990-08-29 Akad Wissenschaften Ddr Verfahren zur herstellung einer thermostabilen, hybriden bacillus-beta-1,3-1,4-glukanase
US5106727A (en) 1989-04-27 1992-04-21 Life Technologies, Inc. Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5556750A (en) * 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
CA2016841C (en) 1989-05-16 1999-09-21 William D. Huse A method for producing polymers having a preselected activity
CA2016842A1 (en) * 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
IT1231157B (it) * 1989-07-20 1991-11-19 Crc Ricerca Chim Nuovi geni ibridi funzionali di bacillus thuringiensis ottenuti mediante ricombinazione in vivo.
US5574205A (en) 1989-07-25 1996-11-12 Cell Genesys Homologous recombination for universal donor cells and chimeric mammalian hosts
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
WO1991006570A1 (en) * 1989-10-25 1991-05-16 The University Of Melbourne HYBRID Fc RECEPTOR MOLECULES
US5071743A (en) * 1989-10-27 1991-12-10 Her Majesty The Queen In Right Of Canada, As Represented By The National Research Council Of Canada Process for conducting site-directed mutagenesis
DE3936258C1 (enExample) * 1989-10-31 1991-04-25 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften Ev, 3400 Goettingen, De
WO1991007506A1 (en) * 1989-11-08 1991-05-30 The United States Of America, Represented By The Secretary, United States Department Of Commerce A method of synthesizing double-stranded dna molecules
US5242937A (en) 1990-03-19 1993-09-07 Research Corporation Technologies, Inc. Topically active ocular thiadiazole sulfonamide carbonic anhydrase inhibitors
EP0527809B1 (en) * 1990-04-05 1995-08-16 CREA, Roberto Walk-through mutagenesis
EP0528881A4 (en) * 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5851813A (en) * 1990-07-12 1998-12-22 President And Fellows Of Harvard College Primate lentivirus antigenic compositions
JP3392863B2 (ja) * 1990-07-24 2003-03-31 エフ．ホフマン ― ラ ロシュ アーゲー 修飾核酸塩基を使用するインビトロ核酸増幅における非特異的増幅の低減
US5264563A (en) 1990-08-24 1993-11-23 Ixsys Inc. Process for synthesizing oligonucleotides with random codons
DE69130647T2 (de) 1990-08-24 1999-05-06 Ixsys, Inc., San Diego, Calif. Verfahren zur herstellung von oligonukleotiden mit regellosen codonen
IL99553A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing oligonucleotides linked to expression elements,a kit for the preparation of vectors useful for the expression of a diverse population of random peptides and methods utilizing the same
US5770434A (en) 1990-09-28 1998-06-23 Ixsys Incorporated Soluble peptides having constrained, secondary conformation in solution and method of making same
US5698426A (en) 1990-09-28 1997-12-16 Ixsys, Incorporated Surface expression libraries of heteromeric receptors
US5871974A (en) 1990-09-28 1999-02-16 Ixsys Inc. Surface expression libraries of heteromeric receptors
US5858725A (en) * 1990-10-10 1999-01-12 Glaxo Wellcome Inc. Preparation of chimaeric antibodies using the recombinant PCR strategy
US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
US5234824A (en) * 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
US5489523A (en) * 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
EP0563296B1 (en) 1990-12-20 1999-03-17 Ixsys, Inc. Optimization of binding proteins
US5098437A (en) 1991-02-13 1992-03-24 Pfizer Hospital Products Group, Inc. Acetabular cup positioning insert
DE4112440C1 (enExample) 1991-04-16 1992-10-22 Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De
US5795747A (en) * 1991-04-16 1998-08-18 Evotec Biosystems Gmbh Process for manufacturing new biopolymers
US5512463A (en) * 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
US5514568A (en) * 1991-04-26 1996-05-07 Eli Lilly And Company Enzymatic inverse polymerase chain reaction
ES2091976T3 (es) 1991-06-20 1996-11-16 Hoffmann La Roche Metodos perfeccionados para la amplificacion del acido nucleico.
WO1993000103A1 (en) * 1991-06-21 1993-01-07 The Wistar Institute Of Anatomy And Biology Chimeric envelope proteins for viral targeting
JP3442774B2 (ja) * 1991-07-01 2003-09-02 バーレックス ラボラトリーズ，インコーポレイティド 新規な突然変異誘発法および組成物
US6071889A (en) * 1991-07-08 2000-06-06 Neurospheres Holdings Ltd. In vivo genetic modification of growth factor-responsive neural precursor cells
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
GB9115364D0 (en) * 1991-07-16 1991-08-28 Wellcome Found Antibody
US5279952A (en) * 1991-08-09 1994-01-18 Board Of Regents, The University Of Texas System PCR-based strategy of constructing chimeric DNA molecules
DE69229477T2 (de) * 1991-09-23 1999-12-09 Cambridge Antibody Technology Ltd., Melbourn Methoden zur Herstellung humanisierter Antikörper
GB9125979D0 (en) * 1991-12-06 1992-02-05 Wellcome Found Antibody
CA2127126A1 (en) * 1992-01-30 1993-08-05 Helen M. Wilks Chiral synthesis with modified enzymes
US5773267A (en) * 1992-02-07 1998-06-30 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University D29 shuttle phasmids and uses thereof
GB9202796D0 (en) * 1992-02-11 1992-03-25 Wellcome Found Antiviral antibody
US5843643A (en) * 1992-02-21 1998-12-01 Ratner; Paul L. Site-specific transfection of eukaryotic cells using polypeptide-linked recombinant nucleic acid
WO1993018141A1 (en) * 1992-03-02 1993-09-16 Biogen, Inc. Thrombin receptor antagonists
ATE280219T1 (de) 1992-03-16 2004-11-15 Jacob Nathaniel Wohlstadter Mutagenese und selektionsmethoden in den selben wirtszellen
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
CA2115346A1 (en) * 1992-06-15 1993-12-23 John E. Shively Chimeric anti-cea antibody
JP2979178B2 (ja) * 1992-07-10 1999-11-15 エナジー バイオシステムズ コーポレーション 脱硫用生物触媒をコードする組換えｄｎａ
US6107062A (en) * 1992-07-30 2000-08-22 Inpax, Inc. Antisense viruses and antisense-ribozyme viruses
FR2694754B1 (fr) * 1992-08-12 1994-09-16 Bio Merieux Fragments d'ADN de mycobactéries, amorces d'amplification, sondes d'hybridation, réactifs et procédé de détection de détection de mycobactéries.
EP0627932B1 (en) * 1992-11-04 2002-05-08 City Of Hope Antibody construct
EP0675953B1 (en) 1992-11-10 2009-01-28 Applied Molecular Evolution, Inc. Soluble peptides having constrained, secondary conformation in solution and method of making same
US5360728A (en) * 1992-12-01 1994-11-01 Woods Hole Oceanographic Institution (W.H.O.I.) Modified apoaequorin having increased bioluminescent activity
DK0672142T3 (da) * 1992-12-04 2001-06-18 Medical Res Council Multivalente og multispecifikke bindingsproteiner samt fremstilling og anvendelse af disse
US5380530A (en) 1992-12-29 1995-01-10 Whitehill Oral Technologies Oral care composition coated gum
IT1264712B1 (it) * 1993-07-13 1996-10-04 Eniricerche Spa Metodo di sintesi biologica di peptidi
US5498531A (en) 1993-09-10 1996-03-12 President And Fellows Of Harvard College Intron-mediated recombinant techniques and reagents
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
DE4343591A1 (de) * 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5521077A (en) * 1994-04-28 1996-05-28 The Leland Stanford Junior University Method of generating multiple protein variants and populations of protein variants prepared thereby
US5514588A (en) 1994-12-13 1996-05-07 Exxon Research And Engineering Company Surfactant-nutrients for bioremediation of hydrocarbon contaminated soils and water
US5629179A (en) * 1995-03-17 1997-05-13 Novagen, Inc. Method and kit for making CDNA library
DE19612631A1 (de) 1995-03-31 1996-10-02 Mazda Motor Multiplexdatenübermittlungssystem
CA2219136A1 (en) * 1995-04-24 1996-10-31 Chromaxome Corp. Methods for generating and screening novel metabolic pathways
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US6168919B1 (en) 1996-07-17 2001-01-02 Diversa Corporation Screening methods for enzymes and enzyme kits
US6030779A (en) 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6004788A (en) 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
DE69620766T2 (de) * 1995-08-11 2004-11-18 Novozymes A/S Verfahren zur herstellung von polypeptidabkömmlingen
US5962258A (en) 1995-08-23 1999-10-05 Diversa Corporation Carboxymethyl cellulase fromthermotoga maritima
US6051409A (en) * 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
US5756316A (en) 1995-11-02 1998-05-26 Genencor International, Inc. Molecular cloning by multimerization of plasmids
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
JP2000502568A (ja) * 1996-01-10 2000-03-07 ノボ ノルディスク アクティーゼルスカブ 細胞内での突然変異ライブラリーの ｉｎ ｖｉｖｏ製造のための方法
US6096548A (en) * 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US6093873A (en) 1996-08-19 2000-07-25 Institut National De La Sante Et De La Recherche Medicale Genetically engineered mice containing alterations in the gene encoding RXR
WO1998028416A1 (en) * 1996-12-20 1998-07-02 Novo Nordisk A/S In vivo recombination
KR20000076363A (ko) * 1997-03-18 2000-12-26 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 Ｄｎａ 라이브러리를 제조하기 위한 시험관내 방법
JP4263248B2 (ja) * 1997-03-18 2009-05-13 ノボザイムス アクティーゼルスカブ Ｄｎａのシャッフリングによるライブラリーの作成方法
AU738461B2 (en) * 1997-03-18 2001-09-20 Novozymes A/S Shuffling of heterologous DNA sequences
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US6051049A (en) 1997-05-29 2000-04-18 Exothermic Distribution Corporation Utilization of strontium aluminate in steelmaking
US6087341A (en) 1998-02-12 2000-07-11 The Board Of Trustees Of The Leland Standford Junior University Introduction of nucleic acid into skin cells by topical application

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