AU2001280968A1 - Compositions and methods for directed gene assembly - Google Patents
Compositions and methods for directed gene assemblyInfo
- Publication number
- AU2001280968A1 AU2001280968A1 AU2001280968A AU8096801A AU2001280968A1 AU 2001280968 A1 AU2001280968 A1 AU 2001280968A1 AU 2001280968 A AU2001280968 A AU 2001280968A AU 8096801 A AU8096801 A AU 8096801A AU 2001280968 A1 AU2001280968 A1 AU 2001280968A1
- Authority
- AU
- Australia
- Prior art keywords
- compositions
- methods
- gene assembly
- directed gene
- directed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22213900P | 2000-07-31 | 2000-07-31 | |
US60222139 | 2000-07-31 | ||
PCT/US2001/024189 WO2002010183A1 (en) | 2000-07-31 | 2001-07-31 | Compositions and methods for directed gene assembly |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2001280968A1 true AU2001280968A1 (en) | 2002-02-13 |
Family
ID=22831012
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001280968A Abandoned AU2001280968A1 (en) | 2000-07-31 | 2001-07-31 | Compositions and methods for directed gene assembly |
Country Status (3)
Country | Link |
---|---|
US (2) | US7087415B2 (en) |
AU (1) | AU2001280968A1 (en) |
WO (1) | WO2002010183A1 (en) |
Families Citing this family (33)
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US7338800B2 (en) * | 2002-01-16 | 2008-03-04 | Baylor College Of Medicine | In vivo gene transfer |
JP4597528B2 (en) * | 2002-02-26 | 2010-12-15 | ジェネンコー・インターナショナル・インク | Subtilisin Carlsberg protein with reduced immunogenicity |
JP4572304B2 (en) * | 2004-01-27 | 2010-11-04 | 独立行政法人情報通信研究機構 | Chemical genetic programming device and chemical genetic programming method |
US11293021B1 (en) | 2016-06-23 | 2022-04-05 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
US20190002874A1 (en) | 2017-06-30 | 2019-01-03 | Inscripta, Inc. | Cell libraries created using rationally designed nucleic acids |
US10557216B2 (en) | 2018-04-24 | 2020-02-11 | Inscripta, Inc. | Automated instrumentation for production of T-cell receptor peptide libraries |
US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
US10501738B2 (en) | 2018-04-24 | 2019-12-10 | Inscripta, Inc. | Automated instrumentation for production of peptide libraries |
CN114854720A (en) | 2018-06-30 | 2022-08-05 | 因思科瑞普特公司 | Apparatus, modules and methods for improved detection of editing sequences in living cells |
US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
US11214781B2 (en) | 2018-10-22 | 2022-01-04 | Inscripta, Inc. | Engineered enzyme |
WO2020086475A1 (en) | 2018-10-22 | 2020-04-30 | Inscripta, Inc. | Engineered enzymes |
CN109903854B (en) * | 2019-01-25 | 2023-04-07 | 电子科技大学 | Core medicine identification method based on traditional Chinese medicine literature |
WO2020180506A1 (en) * | 2019-02-25 | 2020-09-10 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
US10815467B2 (en) | 2019-03-25 | 2020-10-27 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
US11001831B2 (en) | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
WO2020247587A1 (en) | 2019-06-06 | 2020-12-10 | Inscripta, Inc. | Curing for recursive nucleic acid-guided cell editing |
AU2020297499A1 (en) | 2019-06-21 | 2022-02-03 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli |
US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
WO2021102059A1 (en) | 2019-11-19 | 2021-05-27 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
CA3157131A1 (en) | 2019-12-10 | 2021-06-17 | Inscripta, Inc. | Novel mad nucleases |
AU2020407048A1 (en) | 2019-12-18 | 2022-06-09 | Inscripta, Inc. | Cascade/dCas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells |
KR20220133257A (en) | 2020-01-27 | 2022-10-04 | 인스크립타 인코포레이티드 | Electroporation modules and instruments |
US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
EP4214314A1 (en) | 2020-09-15 | 2023-07-26 | Inscripta, Inc. | Crispr editing to embed nucleic acid landing pads into genomes of live cells |
US11512297B2 (en) | 2020-11-09 | 2022-11-29 | Inscripta, Inc. | Affinity tag for recombination protein recruitment |
EP4271802A1 (en) | 2021-01-04 | 2023-11-08 | Inscripta, Inc. | Mad nucleases |
EP4274890A1 (en) | 2021-01-07 | 2023-11-15 | Inscripta, Inc. | Mad nucleases |
US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
Family Cites Families (53)
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US4760025A (en) * | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
US5310675A (en) * | 1983-06-24 | 1994-05-10 | Genencor, Inc. | Procaryotic carbonyl hydrolases |
US5972682A (en) * | 1984-05-29 | 1999-10-26 | Genencor International, Inc. | Enzymatically active modified subtilisins |
US5346823A (en) * | 1984-05-29 | 1994-09-13 | Genencor, Inc. | Subtilisin modifications to enhance oxidative stability |
US5185258A (en) * | 1984-05-29 | 1993-02-09 | Genencor International, Inc. | Subtilisin mutants |
US5801038A (en) * | 1984-05-29 | 1998-09-01 | Genencor International Inc. | Modified subtilisins having amino acid alterations |
US5182204A (en) * | 1984-05-29 | 1993-01-26 | Genencor International, Inc. | Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors |
US5204015A (en) * | 1984-05-29 | 1993-04-20 | Genencor International, Inc. | Subtilisin mutants |
US5155033A (en) * | 1989-01-06 | 1992-10-13 | Genencor, Inc. | Subtilisins modified at position 225 resulting in a shift in catalytic activity |
DE3590766C2 (en) * | 1985-03-30 | 1991-01-10 | Marc Genf/Geneve Ch Ballivet | |
EP0208491B1 (en) * | 1985-07-03 | 1993-08-25 | Genencor International, Inc. | Hybrid polypeptides and process for their preparation |
WO1987004461A1 (en) * | 1986-01-15 | 1987-07-30 | Amgen | THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF |
US4980288A (en) * | 1986-02-12 | 1990-12-25 | Genex Corporation | Subtilisin with increased thermal stability |
US4990452A (en) * | 1986-02-12 | 1991-02-05 | Genex Corporation | Combining mutations for stabilization of subtilisin |
US5763192A (en) * | 1986-11-20 | 1998-06-09 | Ixsys, Incorporated | Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique |
US4914031A (en) * | 1987-04-10 | 1990-04-03 | Amgen, Inc. | Subtilisin analogs |
JPH03505976A (en) * | 1989-05-17 | 1991-12-26 | アムジエン・インコーポレーテツド | Multiple mutant subtilisin |
US5665587A (en) * | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
US5674833A (en) * | 1990-09-18 | 1997-10-07 | Novo Nordisk A/S | Detergent compositions containing protease and novel inhibitors for use therein |
US5482849A (en) * | 1990-12-21 | 1996-01-09 | Novo Nordisk A/S | Subtilisin mutants |
US5340735A (en) * | 1991-05-29 | 1994-08-23 | Cognis, Inc. | Bacillus lentus alkaline protease variants with increased stability |
JP3507073B2 (en) | 1992-03-24 | 2004-03-15 | ケンブリッジ アンティボディー テクノロジー リミティド | Methods for producing members of a specific binding pair |
US5316935A (en) * | 1992-04-06 | 1994-05-31 | California Institute Of Technology | Subtilisin variants suitable for hydrolysis and synthesis in organic media |
US5512452A (en) * | 1993-05-06 | 1996-04-30 | President And Fellows Of Harvard College | Selection of bacterial genes induced in host tissues |
US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5837458A (en) * | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US5521077A (en) * | 1994-04-28 | 1996-05-28 | The Leland Stanford Junior University | Method of generating multiple protein variants and populations of protein variants prepared thereby |
US5985598A (en) * | 1994-10-27 | 1999-11-16 | Thomas Jefferson University | TCL-1 gene and protein and related methods and compositions |
ES2181900T3 (en) * | 1995-06-07 | 2003-03-01 | Invitrogen Corp | RECOMBINANT CLONING IN VITRO USANTO RECOMBINATION SITES BY GENETIC ENGINEERING. |
US6057103A (en) * | 1995-07-18 | 2000-05-02 | Diversa Corporation | Screening for novel bioactivities |
US6030779A (en) * | 1995-07-18 | 2000-02-29 | Diversa Corporation | Screening for novel bioactivities |
US6004788A (en) * | 1995-07-18 | 1999-12-21 | Diversa Corporation | Enzyme kits and libraries |
US5958672A (en) * | 1995-07-18 | 1999-09-28 | Diversa Corporation | Protein activity screening of clones having DNA from uncultivated microorganisms |
JP4156666B2 (en) | 1995-08-11 | 2008-09-24 | ノボザイムス アクティーゼルスカブ | Methods for preparing polypeptide variants |
US5965408A (en) * | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
US5939250A (en) * | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
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US6096548A (en) * | 1996-03-25 | 2000-08-01 | Maxygen, Inc. | Method for directing evolution of a virus |
EP2261373A3 (en) | 1997-01-17 | 2011-12-14 | Codexis Mayflower Holdings, LLC | Evolution of whole cells and organisms by recursive sequence recombination |
US6153410A (en) | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
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WO1999029902A1 (en) | 1997-12-08 | 1999-06-17 | California Institute Of Technology | Method for creating polynucleotide and polypeptide sequences |
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JP2002522089A (en) | 1998-08-12 | 2002-07-23 | マキシジェン, インコーポレイテッド | DNA shuffling to produce herbicide-selective crops |
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CA2345203A1 (en) | 1998-10-07 | 2000-04-13 | Maxygen Inc. | Dna shuffling to produce nucleic acids for mycotoxin detoxification |
EP1129185A1 (en) | 1998-11-10 | 2001-09-05 | Maxygen, Inc. | Modified phosphoenolpyruvate carboxylase for improvement and optimization of plant phenotypes |
WO2000028008A1 (en) | 1998-11-10 | 2000-05-18 | Maxygen, Inc. | Modified ribulose 1,5-bisphosphate carboxylase/oxygenase |
WO2000028018A1 (en) | 1998-11-10 | 2000-05-18 | Maxygen, Inc. | Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes |
US20020150898A1 (en) * | 2000-04-18 | 2002-10-17 | Tang Y. Tom | Novel nucleic acids and polypeptides |
-
2001
- 2001-07-31 WO PCT/US2001/024189 patent/WO2002010183A1/en active Application Filing
- 2001-07-31 AU AU2001280968A patent/AU2001280968A1/en not_active Abandoned
- 2001-07-31 US US09/920,118 patent/US7087415B2/en not_active Expired - Fee Related
-
2006
- 2006-05-12 US US11/433,614 patent/US20060205019A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US20020102734A1 (en) | 2002-08-01 |
US7087415B2 (en) | 2006-08-08 |
US20060205019A1 (en) | 2006-09-14 |
WO2002010183A1 (en) | 2002-02-07 |
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