GB2388604B - A method for in vitro molecular evolution of protein function - Google Patents

A method for in vitro molecular evolution of protein function

Info

Publication number
GB2388604B
GB2388604B GB0211369A GB0211369A GB2388604B GB 2388604 B GB2388604 B GB 2388604B GB 0211369 A GB0211369 A GB 0211369A GB 0211369 A GB0211369 A GB 0211369A GB 2388604 B GB2388604 B GB 2388604B
Authority
GB
United Kingdom
Prior art keywords
protein function
molecular evolution
vitro molecular
vitro
evolution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
GB0211369A
Other versions
GB0211369D0 (en
GB2388604A (en
Inventor
Roland Carlsson
Christina Furebring
Carl Arne Krister Borrebaeck
Hager Ann-Christin Malmborg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alligator Bioscience AB
Original Assignee
Alligator Bioscience AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alligator Bioscience AB filed Critical Alligator Bioscience AB
Priority to GB0211369A priority Critical patent/GB2388604B/en
Publication of GB0211369D0 publication Critical patent/GB0211369D0/en
Priority to DE60312507T priority patent/DE60312507T3/en
Priority to EP03722867A priority patent/EP1504098B2/en
Priority to PT03722867T priority patent/PT1504098E/en
Priority to DK03722867.3T priority patent/DK1504098T4/en
Priority to AU2003230027A priority patent/AU2003230027A1/en
Priority to CA2485506A priority patent/CA2485506C/en
Priority to JP2004506491A priority patent/JP4372679B2/en
Priority to PCT/GB2003/002102 priority patent/WO2003097834A2/en
Priority to ES03722867T priority patent/ES2285118T5/en
Priority to US10/514,399 priority patent/US7262012B2/en
Priority to AT03722867T priority patent/ATE356871T1/en
Publication of GB2388604A publication Critical patent/GB2388604A/en
Priority to DK200401821A priority patent/DK200401821A/en
Application granted granted Critical
Publication of GB2388604B publication Critical patent/GB2388604B/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
GB0211369A 2002-05-17 2002-05-17 A method for in vitro molecular evolution of protein function Expired - Lifetime GB2388604B (en)

Priority Applications (13)

Application Number Priority Date Filing Date Title
GB0211369A GB2388604B (en) 2002-05-17 2002-05-17 A method for in vitro molecular evolution of protein function
PT03722867T PT1504098E (en) 2002-05-17 2003-05-16 A method for in vitro molecular evolution of protein function
US10/514,399 US7262012B2 (en) 2002-05-17 2003-05-16 Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations
DE60312507T DE60312507T3 (en) 2002-05-17 2003-05-16 A METHOD FOR THE IN VITRO MOLECULAR EVOLUTION OF THE PROTEIN FUNCTION
DK03722867.3T DK1504098T4 (en) 2002-05-17 2003-05-16 Process for molecular evolution in vitro of protein function
AU2003230027A AU2003230027A1 (en) 2002-05-17 2003-05-16 A method for in vitro molecular evolution of protein function
CA2485506A CA2485506C (en) 2002-05-17 2003-05-16 A method for in vitro molecular evolution of protein function
JP2004506491A JP4372679B2 (en) 2002-05-17 2003-05-16 In vitro molecular evolution of protein function
PCT/GB2003/002102 WO2003097834A2 (en) 2002-05-17 2003-05-16 A method for in vitro molecular evolution of protein function
ES03722867T ES2285118T5 (en) 2002-05-17 2003-05-16 A METHOD FOR IN VITRO MOLECULAR DEVELOPMENT OF A PROTEIC FUNCTION.
EP03722867A EP1504098B2 (en) 2002-05-17 2003-05-16 A method for in vitro molecular evolution of protein function
AT03722867T ATE356871T1 (en) 2002-05-17 2003-05-16 A METHOD FOR IN VITRO MOLECULAR EVOLUTION OF PROTEIN FUNCTION
DK200401821A DK200401821A (en) 2002-05-17 2004-11-23 Process for in vitro molecular evolution of protein function

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB0211369A GB2388604B (en) 2002-05-17 2002-05-17 A method for in vitro molecular evolution of protein function

Publications (3)

Publication Number Publication Date
GB0211369D0 GB0211369D0 (en) 2002-06-26
GB2388604A GB2388604A (en) 2003-11-19
GB2388604B true GB2388604B (en) 2005-01-26

Family

ID=9936894

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0211369A Expired - Lifetime GB2388604B (en) 2002-05-17 2002-05-17 A method for in vitro molecular evolution of protein function

Country Status (2)

Country Link
GB (1) GB2388604B (en)
PT (1) PT1504098E (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997020078A1 (en) * 1995-11-30 1997-06-05 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1998058080A1 (en) * 1997-06-16 1998-12-23 Bioinvent International Ab A method for in vitro molecular evolution of protein function
WO2002006469A2 (en) * 2000-07-18 2002-01-24 Enchira Biotechnology Corporation Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997020078A1 (en) * 1995-11-30 1997-06-05 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1998058080A1 (en) * 1997-06-16 1998-12-23 Bioinvent International Ab A method for in vitro molecular evolution of protein function
WO2002006469A2 (en) * 2000-07-18 2002-01-24 Enchira Biotechnology Corporation Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GENE, Vol. 243, 2000, KIKUCHI ET AL, "An effective family shuffling method...", pp. 133-137 *

Also Published As

Publication number Publication date
PT1504098E (en) 2007-06-26
GB0211369D0 (en) 2002-06-26
GB2388604A (en) 2003-11-19

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Legal Events

Date Code Title Description
S73 Revocation on comptroller's initiative (section 73/patents act 1977)

Free format text: PATENT REVOKED; PATENT REVOKED UNDER 73(2) ON 20 OCTOBER 2015