DE3936258C1 - - Google Patents

Info

Publication number
DE3936258C1
DE3936258C1 DE19893936258 DE3936258A DE3936258C1 DE 3936258 C1 DE3936258 C1 DE 3936258C1 DE 19893936258 DE19893936258 DE 19893936258 DE 3936258 A DE3936258 A DE 3936258A DE 3936258 C1 DE3936258 C1 DE 3936258C1
Authority
DE
Germany
Prior art keywords
dna
strand
dna molecule
mutagenesis
exonuclease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE19893936258
Other languages
German (de)
English (en)
Inventor
Fritz Prof. Dr. Eckstein
David 3400 Goettingen De Olsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority to DE19893936258 priority Critical patent/DE3936258C1/de
Priority to PCT/EP1990/001939 priority patent/WO1991006645A1/de
Application granted granted Critical
Publication of DE3936258C1 publication Critical patent/DE3936258C1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE19893936258 1989-10-31 1989-10-31 Expired - Lifetime DE3936258C1 (enExample)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE19893936258 DE3936258C1 (enExample) 1989-10-31 1989-10-31
PCT/EP1990/001939 WO1991006645A1 (de) 1989-10-31 1990-10-30 Verfahren zur mutagenese von doppelsträngiger zirkulärer dna

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19893936258 DE3936258C1 (enExample) 1989-10-31 1989-10-31

Publications (1)

Publication Number Publication Date
DE3936258C1 true DE3936258C1 (enExample) 1991-04-25

Family

ID=6392593

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19893936258 Expired - Lifetime DE3936258C1 (enExample) 1989-10-31 1989-10-31

Country Status (2)

Country Link
DE (1) DE3936258C1 (enExample)
WO (1) WO1991006645A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10030376A1 (de) * 2000-06-21 2002-01-24 Bayer Ag Neues Verfahren zum Auffinden neuer Wirkstoffe

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9320459D0 (en) * 1993-10-05 1993-11-24 Nbl Gene Sciences Ltd In vitro method for single-standed dna preparation
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
IL136574A0 (en) 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
AU1098001A (en) * 1999-10-19 2001-04-30 Enchira Biotechnology Corporation Method for directed evolution by random chimeragenesis on transient templates
WO2001029212A1 (en) * 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Methods for chimeragenesis of whole genomes or large polynucleotides
GB0018120D0 (en) * 2000-07-24 2000-09-13 Fermentas Ab Nuclease
GB0025144D0 (en) * 2000-10-13 2000-11-29 Medical Res Council Concatenated nucleic acid sequences
WO2004072252A2 (en) * 2003-02-06 2004-08-26 Rensselaer Polytechnic Institute Polymerase-based protocols for the introduction of deletions and insertions

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Gene 69, S. 325-330, 1988 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10030376A1 (de) * 2000-06-21 2002-01-24 Bayer Ag Neues Verfahren zum Auffinden neuer Wirkstoffe

Also Published As

Publication number Publication date
WO1991006645A1 (de) 1991-05-16

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DE3936258C1 (enExample)
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Legal Events

Date Code Title Description
8100 Publication of the examined application without publication of unexamined application
D1 Grant (no unexamined application published) patent law 81
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee