DE69615406D1 - Verfahren zur herstellung von polynukleotiden mit gewünschten eigenschaften durch iterative selektion und rekombination - Google Patents

Verfahren zur herstellung von polynukleotiden mit gewünschten eigenschaften durch iterative selektion und rekombination

Info

Publication number
DE69615406D1
DE69615406D1 DE69615406T DE69615406T DE69615406D1 DE 69615406 D1 DE69615406 D1 DE 69615406D1 DE 69615406 T DE69615406 T DE 69615406T DE 69615406 T DE69615406 T DE 69615406T DE 69615406 D1 DE69615406 D1 DE 69615406D1
Authority
DE
Germany
Prior art keywords
polynucleotides
sequence
producing
template polynucleotide
recombined
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69615406T
Other languages
English (en)
Other versions
DE69615406T2 (de
DE69615406T3 (de
Inventor
Willem P C Stemmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Maxygen Inc
Original Assignee
Maxygen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27073702&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69615406(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US08/564,955 external-priority patent/US5811238A/en
Application filed by Maxygen Inc filed Critical Maxygen Inc
Publication of DE69615406D1 publication Critical patent/DE69615406D1/de
Publication of DE69615406T2 publication Critical patent/DE69615406T2/de
Application granted granted Critical
Publication of DE69615406T3 publication Critical patent/DE69615406T3/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/02006—Beta-lactamase (3.5.2.6)
    • H—ELECTRICITY
    • H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10N—ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10N60/00—Superconducting devices
    • H10N60/10—Junction-based devices
    • H10N60/128—Junction-based devices having three or more electrodes, e.g. transistor-like structures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
DE69615406T 1995-11-30 1996-12-02 Verfahren zur herstellung von polynukleotiden mit gewünschten eigenschaften durch iterative selektion und rekombination Expired - Lifetime DE69615406T3 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US564955 1995-11-30
US08/564,955 US5811238A (en) 1994-02-17 1995-11-30 Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US08/621,859 US6117679A (en) 1994-02-17 1996-03-25 Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US621859 1996-03-25
PCT/US1996/019256 WO1997020078A1 (en) 1995-11-30 1996-12-02 Methods for generating polynucleotides having desired characteristics by iterative selection and recombination

Publications (3)

Publication Number Publication Date
DE69615406D1 true DE69615406D1 (de) 2001-10-25
DE69615406T2 DE69615406T2 (de) 2002-06-20
DE69615406T3 DE69615406T3 (de) 2010-05-20

Family

ID=27073702

Family Applications (4)

Application Number Title Priority Date Filing Date
DE0911396T Pending DE911396T1 (de) 1995-11-30 1996-12-02 Verfahren zur Herstellung von Polynucleotiden mit erwünschten Eigenschaften mittels wiederholter Selektion und Rekombination
DE0876509T Pending DE876509T1 (de) 1995-11-30 1996-12-02 Verfahren zur herstellung von polynukleotiden mit gewünschten eigenschaftendurch iterative selektion und rekombination
DE69615406T Expired - Lifetime DE69615406T3 (de) 1995-11-30 1996-12-02 Verfahren zur herstellung von polynukleotiden mit gewünschten eigenschaften durch iterative selektion und rekombination
DE69615411T Expired - Fee Related DE69615411T2 (de) 1995-11-30 1996-12-02 Verfahren zur Herstellung von Polynucleotiden mit erwünschten Eigenschaften mittels wiederholter Selektion und Rekombination

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DE0911396T Pending DE911396T1 (de) 1995-11-30 1996-12-02 Verfahren zur Herstellung von Polynucleotiden mit erwünschten Eigenschaften mittels wiederholter Selektion und Rekombination
DE0876509T Pending DE876509T1 (de) 1995-11-30 1996-12-02 Verfahren zur herstellung von polynukleotiden mit gewünschten eigenschaftendurch iterative selektion und rekombination

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69615411T Expired - Fee Related DE69615411T2 (de) 1995-11-30 1996-12-02 Verfahren zur Herstellung von Polynucleotiden mit erwünschten Eigenschaften mittels wiederholter Selektion und Rekombination

Country Status (11)

Country Link
US (8) US6117679A (de)
EP (4) EP1103606A3 (de)
JP (3) JP4015193B2 (de)
AT (2) ATE205877T1 (de)
AU (1) AU713952B2 (de)
CA (1) CA2239099C (de)
DE (4) DE911396T1 (de)
DK (2) DK0911396T3 (de)
ES (2) ES2164929T5 (de)
IL (1) IL124675A0 (de)
WO (1) WO1997020078A1 (de)

Families Citing this family (621)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6808904B2 (en) 1994-06-16 2004-10-26 Syngenta Participations Ag Herbicide-tolerant protox genes produced by DNA shuffling
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6562594B1 (en) 1999-09-29 2003-05-13 Diversa Corporation Saturation mutagenesis in directed evolution
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US20020028443A1 (en) * 1999-09-27 2002-03-07 Jay M. Short Method of dna shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process
US6537776B1 (en) * 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
WO2000053744A2 (en) * 1999-03-09 2000-09-14 Diversa Corporation End selection in directed evolution
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US7018793B1 (en) 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6764835B2 (en) 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6696251B1 (en) 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
DE59712943D1 (de) 1996-07-10 2008-07-10 Lonza Ag Verfahren zur herstellung von (s)- oder (r)-3,3,3-trifluor-2-hydroxy-2-methylpropionsaüre
US5955275A (en) 1997-02-14 1999-09-21 Arcaris, Inc. Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US6025485A (en) 1997-02-14 2000-02-15 Arcaris, Inc. Methods and compositions for peptide libraries displayed on light-emitting scaffolds
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
EP2261373A3 (de) 1997-01-17 2011-12-14 Codexis Mayflower Holdings, LLC Evolution prokaryotischer ganzer Zellen durch rekursive Sequenzrekombination
US7148054B2 (en) * 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
GB9701425D0 (en) 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6623922B1 (en) 1997-02-14 2003-09-23 Deltagen Proteomics Methods for identifying, characterizing, and evolving cell-type specific CIS regulatory elements
US6159688A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods of producing polynucleotide variants
US6159687A (en) * 1997-03-18 2000-12-12 Novo Nordisk A/S Methods for generating recombined polynucleotides
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
DE69806586T2 (de) * 1997-04-09 2003-03-27 Danisco As Kopenhagen Koebenha Verbessertes verfahren zur herstellung von mehlteigwaren sowie produkte hergestellt aus solchen teigwaren unter verwendung von glyzerinoxidase
US6046925A (en) * 1997-04-14 2000-04-04 The Regents Of The University Of California Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
DE19725371B4 (de) * 1997-06-16 2005-01-20 Bornholdt, Stefan, Dr. Verfahren zur Beschleunigung der evolutiven Optimierung von Biopolymeren und zur Verbesserung der damit hergestellten Biopolymere
DE19737562A1 (de) * 1997-08-28 1999-05-06 Otogene Biotechnologische Fors Verfahren zur Identifizierung von Wechselwirkungen zwischen Proteinen bzw. Peptiden
GB9722131D0 (en) * 1997-10-20 1997-12-17 Medical Res Council Method
US6399383B1 (en) 1997-10-28 2002-06-04 Maxygen, Inc. Human papilloma virus vectors
US6596539B1 (en) * 1997-10-31 2003-07-22 Maxygen, Inc. Modification of virus tropism and host range by viral genome shuffling
US6156509A (en) * 1997-11-12 2000-12-05 Genencor International, Inc. Method of increasing efficiency of directed evolution of a gene using phagemid
WO1999029902A1 (en) * 1997-12-08 1999-06-17 California Institute Of Technology Method for creating polynucleotide and polypeptide sequences
AR017831A1 (es) * 1997-12-10 2001-10-24 Pioneer Hi Bred Int Metodo para alterar la composicion de aminoacidos de una proteina nativa de interes, proteina elaborada, y polinucleotido
AU2674299A (en) * 1998-02-11 1999-08-30 Maxygen, Inc. Targeting of genetic vaccine vectors
US6541011B2 (en) 1998-02-11 2003-04-01 Maxygen, Inc. Antigen library immunization
EP1555316A3 (de) * 1998-02-11 2008-01-30 Maxygen, Inc. Antigenbibliothek Immunisierung
EP1518927A3 (de) * 1998-02-11 2005-06-22 Maxygen, Inc. Optimisierung von Immunmodulierungsfähigkeiten von genetischen Impfstoffe
US7390619B1 (en) * 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
CA2320958A1 (en) * 1998-02-11 1999-08-19 Maxygen, Inc. Antigen library immunization
DE19805334A1 (de) * 1998-02-11 1999-08-12 Otogene Biotechnologische Fors Verfahren zur Entwicklung eines pharmazeutischen Wirkstoffes
WO1999041393A1 (en) * 1998-02-17 1999-08-19 Zeneca Limited Insecticidal peptides
CN1314911A (zh) 1998-05-01 2001-09-26 麦克西根股份有限公司 用dna改组优化害虫抗性基因
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
US7153655B2 (en) * 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
EP1090024A2 (de) 1998-06-17 2001-04-11 Maxygen, Inc. Methode für die herstellung von polynukleotiden mit gewünschten eigenschaften
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
KR20010071613A (ko) * 1998-06-29 2001-07-28 추후기재 매우 다양한 라이브러리의 생산방법
ES2188190T5 (es) 1998-07-21 2007-11-16 Danisco A/S Producto alimentario.
MXPA01000224A (es) * 1998-07-28 2002-10-17 California Inst Of Techn Expresion de proteinas eucarioticas funcionales.
US20040191772A1 (en) * 1998-08-12 2004-09-30 Dupret Daniel Marc Method of shuffling polynucleotides using templates
US20030092023A1 (en) * 1998-08-12 2003-05-15 Daniel Dupret Method of shuffling polynucleotides using templates
US6991922B2 (en) * 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
US20030104417A1 (en) * 1998-08-12 2003-06-05 Proteus S.A. Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides
JP2002522089A (ja) * 1998-08-12 2002-07-23 マキシジェン， インコーポレイテッド 除草剤選択性作物を生成するためのｄｎａシャッフリング
US6951719B1 (en) * 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
CA2332615A1 (en) 1998-08-12 2000-02-24 Maxygen Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
DK1115862T3 (da) 1998-09-23 2009-11-16 Zymogenetics Inc Cytokinreceptor Zalpha11
CA2345203A1 (en) * 1998-10-07 2000-04-13 Maxygen Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
WO2000028008A1 (en) * 1998-11-10 2000-05-18 Maxygen, Inc. Modified ribulose 1,5-bisphosphate carboxylase/oxygenase
WO2000028018A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes
IT1303776B1 (it) * 1998-11-19 2001-02-23 S I S S A Scuola Internaz Supe Processo per la preparazione di genoteche, di polipeptidi utilizzandodette genoteche e i polipepti ottenuti.
US6438561B1 (en) * 1998-11-19 2002-08-20 Navigation Technologies Corp. Method and system for using real-time traffic broadcasts with navigation systems
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
JP2002537762A (ja) * 1999-01-05 2002-11-12 トラスティーズ オブ ボストン ユニバーシティ 改良型核酸クローニング
US20040005673A1 (en) * 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
DK1642973T3 (da) 1999-01-07 2009-11-02 Zymogenetics Inc Terapeutisk anvendelser af BR43X2-oplöselige receptorer
EP1151409A1 (de) * 1999-01-18 2001-11-07 Maxygen, Inc. Verfahren zum bevölkern von datenstrukturen für evolutionäre-simulationen
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20030054390A1 (en) * 1999-01-19 2003-03-20 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6917882B2 (en) * 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
JP4899024B2 (ja) * 1999-01-18 2012-03-21 コデクシス メイフラワー ホールディングス， エルエルシー 進化シュミレーションにおける使用のためにデータ構造を居住させる方法
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US7873477B1 (en) 2001-08-21 2011-01-18 Codexis Mayflower Holdings, Llc Method and system using systematically varied data libraries
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
KR20010042037A (ko) * 1999-01-19 2001-05-25 맥시겐, 인크. 목적으로 하는 특징을 갖는 문자열인, 폴리뉴클레오타이드및 폴리펩타이드의 제작 방법
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
EP1108783A3 (de) 1999-01-19 2001-09-05 Maxygen, Inc. Oligonukletiden-gesteuerte Nukleinsäurerekombination
US8457903B1 (en) 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
US20070065838A1 (en) * 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20090130718A1 (en) * 1999-02-04 2009-05-21 Diversa Corporation Gene site saturation mutagenesis
MXPA00009723A (es) * 1999-02-04 2003-09-22 Diversa Corp Generacion no estocastica de vacunas y enzimas geneticas.
US7384387B1 (en) 1999-02-11 2008-06-10 Maxygen, Inc. High throughput mass spectrometry
JP3399518B2 (ja) * 1999-03-03 2003-04-21 インターナショナル・ビジネス・マシーンズ・コーポレーション 半導体構造およびその製造方法
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
WO2000052155A2 (en) * 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids
ATE377076T1 (de) 1999-03-09 2007-11-15 Zymogenetics Inc Humanes cytokin als ligand des zalpha rezeptors und dessen verwendungen
US8148110B2 (en) * 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
AU2005225057A1 (en) * 1999-03-26 2005-12-01 Bp Corporation North America Inc. Synthetic ligation reassembly in directed evolution
MXPA00011569A (es) * 1999-03-26 2004-12-03 Diversa Corp Re-ensamble de acidos nucleicos mediado por exonucleasa en evolucion dirigida.
US20040137515A1 (en) * 1999-06-21 2004-07-15 Gary Lynch Methods and device for in vitro detection and characterization of psychoactives using analysis of repetitive electrical activity in a neuronal sample
CA2377084A1 (en) * 1999-06-25 2001-01-04 Doris Apt Methods and compositions for engineering of attenuated vaccines
AU6069800A (en) 1999-07-07 2001-01-30 Zymogenetics Inc. Human cytokine receptor
WO2001004287A1 (en) * 1999-07-07 2001-01-18 Maxygen Aps A method for preparing modified polypeptides
CZ304402B6 (cs) 1999-08-12 2014-04-16 Zoetis P Llc Polynukleotidová molekula, vektor a hostitelská buňka, mutovaný kmen S. avermitilis a způsob produkce avermektinů
US6797510B1 (en) 2001-05-24 2004-09-28 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US6586230B1 (en) 2000-10-27 2003-07-01 Lexicon Genetics Incorporated Human kinase and polynucleotides encoding the same
US20080050809A1 (en) * 1999-09-28 2008-02-28 Alejandro Abuin Novel human kinases and polynucleotides encoding the same
US20040002474A1 (en) * 1999-10-07 2004-01-01 Maxygen Inc. IFN-alpha homologues
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
WO2001032829A2 (en) * 1999-11-05 2001-05-10 Novozymes A/S Methods to screen microorganisms or gene libraries for products secreted from a cell
DE19953854C2 (de) * 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) * 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
EP1242600B1 (de) 1999-12-23 2010-03-03 ZymoGenetics, Inc. Zytokin zcyt018
SK11672002A3 (sk) 2000-01-10 2002-12-03 Maxygen Holdings Ltd. Polypeptidový konjugát, spôsob jeho výroby, farmaceutický prostriedok s jeho obsahom a jeho použitie
WO2001051663A2 (en) * 2000-01-11 2001-07-19 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
PL207026B1 (pl) 2000-02-09 2010-10-29 Basf Ag Wyizolowany kwas nukleinowy, konstrukt genowy zawierający ten kwas, sekwencja aminokwasowa kodowana przez wyizolowaną sekwencję tego kwasu nukleinowego, wektor zawierający kwas nukleinowy lub konstrukt genowy, roślina transgeniczna lub mikroorganizm transgeniczny zawierający kwas nukleinowy, konstrukt genowy lub wektor, przeciwciało specyficznie wiążące się z polipeptydem kodowanym przez kwas nukleinowy, cząsteczka antysensownego kwasu nukleinowego oraz sposób wytwarzania wielonienasyconych kwasów tłuszczowych (PUFA)
JP2003521930A (ja) 2000-02-11 2003-07-22 マキシゲン・エイピーエス 第ＶＩＩ因子または第ＶＩＩａ因子様分子
WO2001064864A2 (en) * 2000-02-28 2001-09-07 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
AU2001247676A1 (en) * 2000-03-21 2001-10-03 Neugenesis Corporation Methods for in vivo diversification of single genes
EP1276861A2 (de) * 2000-03-24 2003-01-22 Maxygen, Inc. Methoden zur modulation zellulärer und organismenspezifischer phenotypen
WO2001075767A2 (en) * 2000-03-30 2001-10-11 Maxygen, Inc. In silico cross-over site selection
AU2001253127A1 (en) 2000-04-05 2001-10-23 Zymogenetics Inc. Soluble zalpha11 cytokine receptors
US7115403B1 (en) 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US20020045175A1 (en) * 2000-05-23 2002-04-18 Zhen-Gang Wang Gene recombination and hybrid protein development
US20030032059A1 (en) * 2000-05-23 2003-02-13 Zhen-Gang Wang Gene recombination and hybrid protein development
US6686188B2 (en) 2000-05-26 2004-02-03 Amersham Plc Polynucleotide encoding a human myosin-like polypeptide expressed predominantly in heart and muscle
US7074590B2 (en) 2000-06-23 2006-07-11 Maxygen, Inc. Chimeric promoters
CA2411828A1 (en) * 2000-06-23 2002-01-03 Maxygen, Inc. Novel co-stimulatory molecules
EP2287296A1 (de) 2000-06-26 2011-02-23 Novozymes A/S Lipolytisches Enzym
AU2001273032A1 (en) 2000-06-26 2002-01-08 Zymogenetics Inc. Cytokine receptor zcytor17
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
WO2002004629A2 (en) * 2000-07-07 2002-01-17 Maxygen, Inc. Molecular breeding of transposable elements
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US6858422B2 (en) * 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
WO2002010183A1 (en) * 2000-07-31 2002-02-07 Menzel, Rolf Compositions and methods for directed gene assembly
US20020132308A1 (en) * 2000-08-24 2002-09-19 Mpep @ Page 300-M Novel constructs and their use in metabolic pathway engineering
BR0113512A (pt) 2000-08-25 2005-05-10 Basf Plant Science Gmbh Polinucleotìdeos de planta que codificam novas prenil proteases
JP2004524811A (ja) * 2000-09-15 2004-08-19 ディバーサ コーポレーション 有機体の混合集団の組み合わせスクリーニング
WO2002031129A2 (en) 2000-10-12 2002-04-18 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US7045283B2 (en) 2000-10-18 2006-05-16 The Regents Of The University Of California Methods of high-throughput screening for internalizing antibodies
US7202070B2 (en) * 2000-10-31 2007-04-10 Biocatalytics, Inc. Method for reductive amination of a ketone using a mutated enzyme
AU2002228633A1 (en) * 2000-11-20 2002-06-03 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
GB2370038B (en) * 2000-12-12 2003-03-26 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
DE60110926T3 (de) * 2000-12-12 2012-06-14 Alligator Bioscience Ab Eine methode zur in vitro molekularen evolution der proteinfunktion
AU2002232642B2 (en) * 2000-12-12 2007-02-01 Lexicon Pharmaceuticals, Inc. Novel human kinases and uses thereof
JP2005503755A (ja) * 2000-12-13 2005-02-10 ニューゲン テクノロジーズ， インコーポレイテッド 核酸配列の複数コピーを作製するための方法および組成物、ならびにそれらを検出する方法
US20020150895A1 (en) * 2000-12-22 2002-10-17 Raymond Wheeler Method and apparatus for filtering and extending RNA alignment coverage
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
AU2002246858A1 (en) * 2000-12-27 2002-08-06 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US6849443B2 (en) * 2001-01-23 2005-02-01 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US7838287B2 (en) * 2001-01-25 2010-11-23 Evolva Sa Library of a collection of cells
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
CA2434881A1 (en) * 2001-02-02 2002-08-15 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Method for identifying functional nucleic acids
US20050003354A1 (en) * 2001-02-21 2005-01-06 Maxygen, Inc. Methods for improving or altering promoter/enhancer properties
CA2416963C (en) * 2001-03-09 2012-01-10 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
WO2002074932A2 (en) 2001-03-20 2002-09-26 Lexicon Genetics Incorporated Novel human kinase and polynucleotides encoding the same
US20030087254A1 (en) * 2001-04-05 2003-05-08 Simon Delagrave Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics
EP1383879A4 (de) 2001-04-06 2005-05-11 Lexicon Genetics Inc Neue menschliche kinasen und diese codierende polynukleotide
US7465567B2 (en) 2001-04-16 2008-12-16 California Institute Of Technology Peroxide-driven cytochrome P450 oxygenase variants
AU2002258997A1 (en) * 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
JP2004528850A (ja) * 2001-05-03 2004-09-24 レンセレアー ポリテクニック インスティテュート 定方向進化の新規方法
JP2004537986A (ja) 2001-05-09 2004-12-24 レキシコン・ジェネティクス・インコーポレーテッド 新規キナーゼおよびそれをコードするポリヌクレオチド
BR0209154A (pt) 2001-05-18 2004-07-20 Danisco Processo de preparação de uma massa com uma enzima
WO2002095073A1 (en) * 2001-05-18 2002-11-28 Wisconsin Alumni Research Foundation Method for the synthesis of dna sequences
CA2387401C (en) * 2001-05-21 2004-10-12 Ecopia Biosciences Inc. Compositions, methods and systems for the production of enediynes
EP2298868B1 (de) 2001-06-26 2015-01-07 Novozymes A/S Polypeptide mit Cellobiohydrolase I-Aktivität und dafür kodierende Polynucleotide
US20030143612A1 (en) * 2001-07-18 2003-07-31 Pointilliste, Inc. Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
US7226768B2 (en) 2001-07-20 2007-06-05 The California Institute Of Technology Cytochrome P450 oxygenases
ATE432981T1 (de) * 2001-07-26 2009-06-15 Stratagene California Multi-stellen-mutagenese
ATE321139T1 (de) * 2001-07-26 2006-04-15 Ecopia Biosciences Inc Gene und proteine zur rosaramicin biosynthese
US6833441B2 (en) * 2001-08-01 2004-12-21 Abmaxis, Inc. Compositions and methods for generating chimeric heteromultimers
US20040078837A1 (en) * 2001-08-02 2004-04-22 Shannon Mark E. Four human zinc-finger-containing proteins: MDZ3, MDZ4, MDZ7 and MDZ12
US6979733B2 (en) * 2001-08-03 2005-12-27 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods of making and using them
US6943001B2 (en) * 2001-08-03 2005-09-13 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods for making and using them
US6987079B2 (en) * 2001-08-14 2006-01-17 W.R. Grace & Co.-Conn. Supported catalyst systems
US7647184B2 (en) * 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
EP1961293A3 (de) 2001-09-27 2009-01-28 Pioneer Hi-Bred International, Inc. Phytatpolynucleotide und Verfahren zu ihrer Verwendung
DE60232672D1 (de) 2001-10-01 2009-07-30 Dyax Corp Mehrkettige eukaryontische display-vektoren und deren verwendungen
DK2279753T3 (en) 2001-10-10 2015-11-23 Novo Nordisk As The conversion of peptides and glycokonjugering
PT2279755E (pt) 2001-10-10 2014-06-04 Ratiopharm Gmbh Remodelação e glicoconjugação do factor de crescimento de fibroblastos (fgf)
US20040005709A1 (en) * 2001-10-24 2004-01-08 Hoogenboom Henricus Renerus Jacobus Mattheus Hybridization control of sequence variation
US7175983B2 (en) * 2001-11-02 2007-02-13 Abmaxis, Inc. Adapter-directed display systems
DK1451322T3 (da) 2001-11-05 2010-02-01 Zymogenetics Inc Il-21-antagonister
GB0126887D0 (en) * 2001-11-08 2002-01-02 Univ London Method for producing and identifying soluble protein domains
CN1697882A (zh) * 2001-11-19 2005-11-16 帕拉里勒生物科学公司 多重pcr
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
WO2003050914A1 (en) * 2001-12-05 2003-06-19 E-Tenna Corporation Capacitively-loaded bent-wire monopole on an artificial magnetic conductor
US20040137547A1 (en) * 2001-12-28 2004-07-15 Medtronic Minimed, Inc. Method for formulating a glucose oxidase enzyme with a desired property or properties and a glucose oxidase enzyme with the desired property
US20040009498A1 (en) * 2002-01-14 2004-01-15 Diversa Corporation Chimeric antigen binding molecules and methods for making and using them
WO2003061570A2 (en) * 2002-01-16 2003-07-31 Zyomyx, Inc. Engineered binding proteins
DK1476541T3 (da) 2002-01-18 2008-11-03 Zymogenetics Inc Cytokin (zcytor17-ligand)
US7494804B2 (en) 2002-01-18 2009-02-24 Zymogenetics, Inc. Polynucleotide encoding cytokine receptor zcytor17 multimer
EP1474504A2 (de) * 2002-01-24 2004-11-10 Pointilliste, Inc. Verwendung von sammlungen von bindungsstellen für die probenprofilierung und andere anwendungen
CA2511229A1 (en) * 2002-01-25 2003-07-31 Evolva Ltd. Methods for multiple parameter screening and evolution of cells to produce small molecules with multiple functionalities
US20040096826A1 (en) * 2002-01-30 2004-05-20 Evans Glen A. Methods for creating recombination products between nucleotide sequences
ATE426659T1 (de) 2002-02-12 2009-04-15 Pfizer Prod Inc Streptomyces avermitilis gen zur regulierung des verhaltnisses der b2:b1 avermectine
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
EP1499738B1 (de) 2002-02-21 2008-07-09 ASM Scientific, Inc. Rekombinase-polymerase-amplifikation
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
CA2476901C (en) 2002-02-22 2012-10-09 Progenics Pharmaceuticals, Inc. Anti-ccr5 antibody
US20030166010A1 (en) * 2002-02-25 2003-09-04 Affholter Joseph A. Custom ligand design for biomolecular filtration and purification for bioseperation
US20030224404A1 (en) * 2002-02-25 2003-12-04 Manuel Vega High throughput directed evolution of nucleic acids by rational mutagenesis
KR20040088538A (ko) * 2002-02-26 2004-10-16 이 아이 듀폰 디 네모아 앤드 캄파니 유전 인자의 재조합 방법
US7220563B2 (en) 2002-02-27 2007-05-22 California Institute Of Technology Glucose 6-oxidases
US7747391B2 (en) * 2002-03-01 2010-06-29 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
DK1493027T3 (en) 2002-03-01 2014-11-17 Codexis Mayflower Holdings Llc Methods, systems and software for identifying functional biomolecules
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
NZ535044A (en) * 2002-03-01 2008-12-24 Ravgen Inc Non-invasive method to determine the genetic sequence of foetal DNA from a sample from a pregnant female thereby detecting any alternation in gene sequence as compared with the wild type sequence
US7620500B2 (en) * 2002-03-09 2009-11-17 Maxygen, Inc. Optimization of crossover points for directed evolution
AU2003222269A1 (en) * 2002-03-11 2003-09-29 Nugen Technologies, Inc. Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination
CN101508725B (zh) 2002-03-22 2013-08-07 拜尔作物科学公司 新的苏云金芽孢杆菌杀昆虫蛋白质
CA2481504C (en) 2002-04-08 2011-08-23 Pioneer Hi-Bred International, Inc. Enhanced silk exsertion under stress
WO2003089587A2 (en) * 2002-04-16 2003-10-30 Promega Corporation Method to enhance homologous recombination
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
PT1497418E (pt) 2002-04-19 2013-01-25 Dsm Ip Assets Bv Fosfolípases, ácidos nucleicos que as codificam e métodos para as preparar e utilizar
EP1497415B1 (de) 2002-04-19 2010-12-29 ZymoGenetics, L.L.C. Verfahren zum nachweis oder zur modulierung der wechselwirkung eines cytokinrezeptors mit seinem ligand
US7208317B2 (en) * 2002-05-02 2007-04-24 The University Of North Carolina At Chapel Hill In Vitro mutagenesis, phenotyping, and gene mapping
FR2839317A1 (fr) * 2002-05-06 2003-11-07 Entomed Procede d'evolution fonctionnelle artificielle de sequences polynucleotidiques, polynucleotides recombines obtenus et peptides, polypeptides et proteines codes par ceux-ci
US7442506B2 (en) * 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US20070178478A1 (en) * 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
GB2388604B (en) * 2002-05-17 2005-01-26 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
JP4372679B2 (ja) * 2002-05-17 2009-11-25 アリゲーター・バイオサイエンス・アーベー タンパク質機能をインヴィトロで分子進化させる方法
EP2377547A1 (de) 2002-06-07 2011-10-19 ZymoGenetics, Inc. Verwendung von IL-21als antimikrobiellen Mittel
GB0213816D0 (en) * 2002-06-14 2002-07-24 Univ Aston Method of producing DNA and protein libraries
AU2003240005B2 (en) * 2002-06-14 2010-08-12 Takeda Pharmaceutical Company Limited Recombination of nucleic acid library members
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
DE10233047A1 (de) * 2002-07-19 2004-02-26 Amaxa Gmbh Verfahren zur Herstellung eines künstlichen Polypeptids und nach diesem Verfahren hergestelltes künstliches Protein
AU2003242516A1 (en) * 2002-07-23 2004-02-09 Nuevolution A/S Gene shuffling by template switching
CA2518588A1 (en) 2002-07-25 2004-02-05 Mj Bioworks Incorporated Hybrid dna polymerase proteins having increased polymerase to exonuclease activity
DK1529110T3 (da) * 2002-08-01 2012-01-30 Evolva Ltd Fremgangsmåde til blanding af store antal af heterologe gener
EP1576151A4 (de) 2002-08-06 2006-05-17 Verdia Inc Ap1-aminoxidase-varianten
US7047591B2 (en) * 2002-09-20 2006-05-23 Colgate-Palmolive Company Oral care implement
ATE466085T1 (de) * 2002-09-09 2010-05-15 Hanall Pharmaceutical Co Ltd Protease-resistente modifizierte interferon alpha polypeptide
US20050202438A1 (en) * 2002-09-09 2005-09-15 Rene Gantier Rational directed protein evolution using two-dimensional rational mutagenesis scanning
US20060020396A1 (en) * 2002-09-09 2006-01-26 Rene Gantier Rational directed protein evolution using two-dimensional rational mutagenesis scanning
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
CA2644185C (en) * 2002-09-20 2013-12-03 Colgate-Palmolive Company Toothbrush with gripping area
AU2002951899A0 (en) * 2002-10-04 2002-10-24 Macquarie University Random drift mutagenesis
JP3447009B1 (ja) * 2002-10-29 2003-09-16 實 平垣 構築物用構成体およびその製造方法
AU2003287384A1 (en) * 2002-10-30 2004-06-07 Pointilliste, Inc. Systems for capture and analysis of biological particles and methods using the systems
US7135290B2 (en) * 2003-04-12 2006-11-14 Solazyme, Inc. Methods and compositions for evolving hydrogenase genes
US7090979B2 (en) * 2002-11-22 2006-08-15 The Regents Of The University Of California Derivatized versions of ligase enzymes for constructing DNA sequences
WO2004056981A2 (en) 2002-12-20 2004-07-08 Novozymes A/S Polypeptides having cellobiohydrolase ii activity and polynucleotides encoding same
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
MXPA05007654A (es) 2003-01-17 2005-09-30 Danisco Metodo.
ATE533849T1 (de) 2003-01-17 2011-12-15 Danisco Verfahren zur in-situ-herstellung eines emulgators in einem nahrungsmittel
US20050196766A1 (en) 2003-12-24 2005-09-08 Soe Jorn B. Proteins
WO2004065401A1 (en) 2003-01-21 2004-08-05 Ecopia Biosciences Inc. Polyene polyketides, processes for their production and their use as a pharmaceutical
DE102004002243A1 (de) * 2003-02-07 2004-09-16 Trikon Technologies Limited, Newport Elektrostatische Klemmhalterung für dünne Wafer in einer Vakuumkammer zur Plasmabearbeitung
EP1597577A4 (de) * 2003-02-10 2007-02-21 Pointilliste Inc Selbstanordnende arrays und ihre anwendung
CN102618564B (zh) 2003-03-06 2014-10-29 维莱尼姆公司 淀粉酶、编码它们的核酸及其制备和应用方法
MX295545B (es) 2003-03-07 2012-02-03 Diversa Corp Hidrolasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas.
US7083931B2 (en) 2003-04-04 2006-08-01 Stratagene California Renilla GFP mutants with increased fluorescent intensity and spectral shift
US7592434B2 (en) 2003-04-04 2009-09-22 Verenium Corporation Pectate lyases, nucleic encoding them and methods for making and using them
EP1615945B1 (de) 2003-04-09 2011-09-28 BioGeneriX AG Glycopegylierungsverfahren und durch die verfahren hergestellte proteine/peptide
WO2004092418A2 (en) * 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
EP2322629A3 (de) 2003-04-29 2011-11-02 Pioneer Hi-Bred International Inc. Neue Glyphosat-N-Acetyltransferase-Gene
US20070073048A1 (en) 2003-05-15 2007-03-29 Ying Lian Hiv polynucleotides and polypeptides derived from botswana mj4
WO2005017105A2 (en) 2003-06-17 2005-02-24 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
WO2005017106A2 (en) 2003-06-17 2005-02-24 California Institute Of Technology Libraries of optimized cytochrome p450 enzymes and the optimized p450 enzymes
US20050048578A1 (en) * 2003-06-26 2005-03-03 Epitomics, Inc. Methods of screening for monoclonal antibodies with desirable activity
JP2007529993A (ja) 2003-07-02 2007-11-01 シンジェンタ パーティシペーションズ アーゲー グルカナーゼ、それらをコードする核酸並びにそれらを製造及び使用する方法
EP1660646B1 (de) 2003-08-11 2014-12-31 California Institute Of Technology Temperaturstabile peroxidgetriebene cytochrom-p450-oxygenase-varianten und verwendungsverfahren
CA2535526C (en) 2003-08-11 2015-09-29 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
ATE510011T1 (de) 2003-08-25 2011-06-15 Funzyme Biotechnologies Sa Neue pilzproteine und diese codierende nukleinsäuren
EP1670932B1 (de) * 2003-08-27 2011-03-30 Proterec Ltd Bibliotheken rekombinanter chimärischer proteine
US20070149496A1 (en) * 2003-10-31 2007-06-28 Jack Tuszynski Water-soluble compound
AU2004312215B2 (en) 2003-12-24 2009-03-05 Dupont Nutrition Biosciences Aps Proteins
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
EP1718742B1 (de) 2004-02-25 2014-12-24 Novozymes A/S Pilzliche zellwand abbauendes enzym
CN101671681B (zh) 2004-02-25 2012-11-28 先锋高级育种国际公司 新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
US7622125B2 (en) * 2004-05-05 2009-11-24 Novartis Vaccines And Diagnostics, Inc. Polycistronic HIV vector constructs
AU2015203225B2 (en) * 2004-06-01 2017-05-04 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification
JP5026958B2 (ja) * 2004-06-01 2012-09-19 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅
AR049214A1 (es) 2004-06-09 2006-07-05 Pioneer Hi Bred Int Peptidos de transito a plastidos
US20100016178A1 (en) * 2004-06-09 2010-01-21 Sussman Michael R Methods for rapid production of target double-stranded dna sequences
BRPI0510939A (pt) 2004-06-16 2007-07-17 Diversa Corp composições e métodos para descoloração enzimática de clorofila
ATE514767T1 (de) 2004-07-16 2011-07-15 Danisco Enzymatisches öldegummierungsverfahren
GB0421598D0 (en) 2004-09-29 2004-10-27 Cambridge Advanced Tech Modification of plant development and morphology
CA2584984A1 (en) * 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US20060204979A1 (en) * 2004-12-01 2006-09-14 Invitrogen Corporation Reverse two-hybrid system for identification of interaction domains
DE102005005438A1 (de) * 2005-02-05 2006-08-24 Bayer Healthcare Ag Mutanten des fluoreszierenden Proteins CGFPs, sowie deren Verwendung
US20060177447A1 (en) 2005-02-08 2006-08-10 Wenfeng Xu Anti-IL-20, anti-IL-22 and anti-IL-22RA antibodies and binding partners and methods of using in inflammation
US20090038023A1 (en) 2005-03-10 2009-02-05 Verenium Corporation Lyase Enzymes, Nucleic Acids Encoding Them and Methods For Making and Using Them
NZ594810A (en) 2005-03-15 2012-12-21 Verenium Corp Cellulases, nucleic acids encoding them and methods for making and using them
US11214817B2 (en) 2005-03-28 2022-01-04 California Institute Of Technology Alkane oxidation by modified hydroxylases
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
US20070009928A1 (en) * 2005-03-31 2007-01-11 Lathrop Richard H Gene synthesis using pooled DNA
US8076108B2 (en) * 2005-04-26 2011-12-13 Cargill, Incorporated Polypeptides and biosynthetic pathways for the production of stereoisomers of monatin and their precursors
ES2609429T3 (es) 2005-05-12 2017-04-20 Zymogenetics, Inc. Composiciones y métodos para modular respuestas inmunitarias
NZ565138A (en) 2005-06-21 2012-02-24 Xoma Technology Ltd IL-1beta Binding antibodies and fragments thereof
US8062850B2 (en) 2005-07-25 2011-11-22 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
EP1929046B1 (de) 2005-09-07 2012-07-11 Nugen Technologies, Inc. Verbessertes nukleinsäureamplifikationsverfahren
CA2630216A1 (en) * 2005-11-18 2007-05-31 Gloucester Pharmaceuticals, Inc. Metabolite derivatives of the hdac inhibitor fk228
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
DE602007013695D1 (de) * 2006-01-11 2011-05-19 Axxam Spa Leuchtende stammzellen und ihre verwendung
EP2216403A3 (de) 2006-02-02 2010-11-24 Verenium Corporation Esterasen und dazugehörige Nukleinsäuren und Verfahren
DK2420570T3 (en) 2006-02-10 2014-03-10 Bp Corp North America Inc Arabinofuranosidaseenzymer, nucleic acids encoding them, and methods for making and using them
EP2548955A1 (de) 2006-02-14 2013-01-23 Verenium Corporation Xylanasen, Nukleinsäuren, die diese codieren, und Verfahren zu deren Herstellung und Verwendung
US8546118B2 (en) 2006-03-07 2013-10-01 Verenium Corporation Aldolases, nucleic acids encoding them and methods for making and using them
BRPI0708673A2 (pt) 2006-03-07 2011-06-07 Cargill Inc método para fabricação de polipeptìdeos com atividade de aldolase e polinucleotìdeos que codificam estes polipeptìdeos
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US20100137239A1 (en) * 2006-04-24 2010-06-03 Gloucester Pharmaceuticals Gemcitabine combination therapy
WO2008035205A2 (en) 2006-05-04 2008-03-27 Asm Scientific, Inc. Recombinase polymerase amplification
WO2007137163A2 (en) 2006-05-19 2007-11-29 Georgia Tech Research Corporation Abc transporter ligand
US8957027B2 (en) * 2006-06-08 2015-02-17 Celgene Corporation Deacetylase inhibitor therapy
US7572618B2 (en) 2006-06-30 2009-08-11 Bristol-Myers Squibb Company Polynucleotides encoding novel PCSK9 variants
US8324158B2 (en) 2006-07-14 2012-12-04 Georgia Tech Research Corporation Methods for inhibiting CLC-2 channel with GATX2
EP2069513A4 (de) 2006-08-04 2012-03-21 California Inst Of Techn Verfahren und systeme zur selektiven fluorinierung organischer moleküle
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
CA3020590A1 (en) 2006-08-04 2009-02-12 Bp Corporation North America Inc. Glucanases, nucleic acids encoding them and methods for making and using them
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
PT2057274E (pt) 2006-09-21 2014-03-13 Dsm Ip Assets Bv Fosfolípases, ácidos nucleicos que as codificam e métodos de preparação e utilização das mesmas
ES2531135T3 (es) 2006-09-21 2015-03-11 Basf Enzymes Llc Fitasas, ácidos nucleicos que las codifican y métodos para su producción y uso
CN101583719B (zh) 2006-12-20 2015-03-25 孟山都巴西有限公司 用于改变纤维长度和/或植物高度的核酸构建体和方法
CN101578370B (zh) 2006-12-20 2015-11-25 孟山都巴西有限公司 编码c3hc4家族植物蛋白质的核酸分子和改变植物纤维素和木素含量的方法
PL2069490T5 (pl) 2006-12-21 2018-09-28 Basf Enzymes Llc Amylazy i glukoamylazy, kwasy nukleinowe kodujące te związki oraz sposoby wytwarzania tych związków oraz stosowania ich
EP2815761A1 (de) * 2006-12-29 2014-12-24 Celgene Corporation Tumorbehandlung auf der basis von romidepsin
WO2008083288A2 (en) * 2006-12-29 2008-07-10 Gloucester Pharmaceuticals Purifiction of romidepsin
DE102007003143A1 (de) 2007-01-16 2008-07-17 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
CA2673954C (en) 2007-01-25 2015-09-15 Danisco A/S Production of a lipid acyltransferase from transformed bacillus licheniformis cells
US20100189706A1 (en) 2007-01-30 2010-07-29 Cathy Chang Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
SG178753A1 (en) 2007-02-12 2012-03-29 Codexis Inc Structure-activity relationships
CN101663047B (zh) 2007-02-23 2014-07-09 纽约哥伦比亚大学理事会 通过激活或阻断HLA-E/Qa-1限制性CD8+T细胞调控途径来治疗免疫疾病的方法
JP5646328B2 (ja) 2007-10-01 2014-12-24 コデクシス， インコーポレイテッド アゼチジノンの生産のためのケトレダクターゼポリペプチド
JP5744518B2 (ja) 2007-10-03 2015-07-08 ビーピー・コーポレーション・ノース・アメリカ・インコーポレーテッド キシラナーゼ、キシラナーゼをコードする核酸並びにそれらを製造及び使用する方法
CA2971794C (en) 2007-10-04 2020-03-24 Zymogenetics, Inc. B7 family member zb7h6 and related compositions and methods
DE102007051092A1 (de) 2007-10-24 2009-04-30 Henkel Ag & Co. Kgaa Subtilisin aus Becillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin
EP2612868B1 (de) 2007-11-01 2018-08-15 Astellas Pharma Inc. Immunsuppressive Polypeptide und Nucleinsäuren
WO2009059379A1 (en) 2007-11-07 2009-05-14 Dynamic Microbials Limited Antimicrobial compositions, formulations and uses thereof
CA2706439C (en) 2007-11-20 2015-04-21 Pioneer Hi-Bred International, Inc. Maize ethylene signaling genes and modulation of same for improved stress tolerance in plants
EP2225267B1 (de) 2007-11-21 2015-01-14 Roskilde Universitet Polypeptide mit ice-bindender aktivität
WO2009065934A1 (en) * 2007-11-22 2009-05-28 Novozymes A/S Polypeptides having ferulic acid esterase activity and polynucleotides encoding same
EA020035B1 (ru) 2007-12-21 2014-08-29 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Способы
DK2238261T3 (da) 2008-01-03 2014-02-10 Verenium Corp Isomeraser, nukleinsyrer der koder for dem og fremgangsmåder til fremstilling og anvendelse deraf
US8709772B2 (en) 2008-01-03 2014-04-29 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
AU2009207645B2 (en) 2008-01-25 2014-11-13 Aarhus Universitet Selective exosite inhibition of PAPP-A activity against IGFBP-4
US8768871B2 (en) * 2008-02-12 2014-07-01 Codexis, Inc. Method of generating an optimized, diverse population of variants
DK2250595T3 (en) 2008-02-12 2017-09-04 Codexis Inc PROCEDURE FOR SELECTING AN OPTIMIZED VARIETY OF VARIETIES
US8034568B2 (en) * 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
CN102066409A (zh) 2008-04-17 2011-05-18 诺维信公司 具有阿魏酸酯酶活性的多肽和编码该多肽的多核苷酸
GB0807161D0 (en) * 2008-04-18 2008-05-21 Danisco Process
AU2009248931A1 (en) 2008-05-23 2009-11-26 E. I. Du Pont De Nemours And Company Novel DGAT genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
KR20110048509A (ko) 2008-06-24 2011-05-11 코덱시스, 인코포레이티드 실질적으로 입체이성질체적으로 순수한 융합 이환식 프롤린 화합물의 제조를 위한 생체촉매 공정
EP2329013B1 (de) * 2008-08-27 2015-10-28 Codexis, Inc. Ketoreduktasepolypeptide zur herstellung von einem 3-aryl-3-hydroxypropanamin aus einem 3-aryl-3-ketopropanamin
WO2010027710A2 (en) 2008-08-27 2010-03-11 Codexis, Inc. Ketoreductase polypeptides and uses thereof
US8288141B2 (en) * 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
US8153391B2 (en) 2008-08-29 2012-04-10 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8357503B2 (en) 2008-08-29 2013-01-22 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8273554B2 (en) 2008-08-29 2012-09-25 Codexis, Inc. Ketoreductase polypeptides for the stereoselective production of (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
US20100081575A1 (en) * 2008-09-22 2010-04-01 Robert Anthony Williamson Methods for creating diversity in libraries and libraries, display vectors and methods, and displayed molecules
MX357418B (es) 2008-09-26 2018-07-09 Tocagen Inc Vectores de terapia genica y citosina deaminasas.
US9403904B2 (en) 2008-11-07 2016-08-02 Fabrus, Inc. Anti-DLL4 antibodies and uses thereof
WO2010054319A2 (en) 2008-11-10 2010-05-14 Codexis, Inc. Penicillin-g acylases
JP5645840B2 (ja) 2008-12-02 2014-12-24 株式会社Ｗａｖｅ Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ Ｊａｐａｎ リン原子修飾核酸の合成方法
EP3936508B1 (de) 2008-12-03 2023-12-06 The Scripps Research Institute Zusammensetzung umfassend stammzellkulturen
CA2743707A1 (en) 2008-12-04 2010-06-10 Pioneer Hi-Bred International, Inc. Methods and compositions for enhanced yield by targeted expression of knotted1
WO2010080635A2 (en) 2008-12-18 2010-07-15 Codexis, Inc. Recombinant halohydrin dehalogenase polypeptides
WO2010075574A2 (en) * 2008-12-25 2010-07-01 Codexis, Inc. Enone reductases
AU2010212851B2 (en) 2009-02-13 2012-12-13 Novartis Ag Nucleic acid molecule of a biosynthetic cluster encoding non ribosomal peptide synthases and uses thereof
CA2752818A1 (en) 2009-02-26 2010-09-02 Codexis, Inc. Beta-glucosidase variant enzymes and related polynucleotides
WO2010099501A2 (en) 2009-02-26 2010-09-02 Codexis, Inc. Transaminase biocatalysts
US8716553B2 (en) 2009-03-02 2014-05-06 Pioneer Hi Bred International Inc NAC transcriptional activators involved in abiotic stress tolerance
CA2754580C (en) 2009-03-17 2014-06-17 Codexis, Inc. Variant endoglucanases and related polynucleotides
EP2412387B1 (de) 2009-03-27 2014-11-19 Proyecto de Biomedicina Cima, S.L. Verfahren und zusammensetzungen zur behandlung von zirrhose und leberfibrose
BRPI1010239A2 (pt) 2009-03-31 2016-10-11 Codexis Inc endoglucanases aprimoradas, derivados e seus usos
US20120058222A1 (en) 2009-05-19 2012-03-08 Danisco A/S Use
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
US9695403B2 (en) 2009-05-21 2017-07-04 Syngenta Participations Ag Phytases, nucleic acids encoding them and methods for making and using them
EP3360974A1 (de) 2009-06-05 2018-08-15 Alere San Diego, Inc. Reagentien zur rekombinase polymerase amplifikation
AR077042A1 (es) 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina
EP2443235A4 (de) 2009-06-16 2013-07-31 Codexis Inc ß-GLUCOSIDASE-VARIANTEN
SG177331A1 (en) 2009-06-22 2012-02-28 Codexis Inc Ketoreductase-mediated stereoselective route to alpha chloroalcohols
EP2446026B1 (de) 2009-06-22 2017-08-09 Codexis, Inc. Transaminasereaktionen
EP2451461A4 (de) 2009-07-06 2013-05-29 Ontorii Inc Neuartige nukleinsäure-prodrugs und verwendungsverfahren dafür
WO2011011630A2 (en) 2009-07-23 2011-01-27 Codexis, Inc. Nitrilase biocatalysts
US20110035843A1 (en) 2009-08-05 2011-02-10 Pioneer Hi-Bred International, Inc. Novel eto1 genes and use of same for reduced ethylene and improved stress tolerance in plants
US9102959B2 (en) 2009-08-19 2015-08-11 Codexis, Inc. Ketoreductase polypeptides for the preparation of phenylephrine
EP2467395A1 (de) 2009-08-20 2012-06-27 Pioneer Hi-Bred International Inc. Funktionale expression eines gemischten hefenitrattransporters (ynti) bei mais zur verbesserung der nitrataufnahme unter nitratarmen bedingungen
EP2473604B1 (de) 2009-09-04 2017-01-11 Codexis, Inc. Variante cbh2-zellulasen und entsprechende polynukleotide
WO2011041594A1 (en) 2009-09-30 2011-04-07 Codexis, Inc. Recombinant c1 b-glucosidase for production of sugars from cellulosic biomass
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
CA2780974C (en) 2009-11-20 2016-02-02 Codexis, Inc. Mutli-cellulase enzyme compositions for hydrolysis of cellulosic biomass
WO2011066457A2 (en) 2009-11-25 2011-06-03 Codexis, Inc. Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
SI2510089T1 (sl) 2009-12-08 2015-12-31 Codexis, Inc. Sinteza prazolnih spojin
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
WO2011100265A2 (en) 2010-02-10 2011-08-18 Codexis, Inc. Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system
PL2545150T3 (pl) 2010-03-12 2019-01-31 Dupont Nutrition Biosciences Aps Sposób
WO2011114251A1 (en) 2010-03-18 2011-09-22 Danisco A/S Foodstuff
EP2553108A4 (de) 2010-03-31 2015-01-28 Codexis Inc Herstellung von monoterpenen
US9040262B2 (en) 2010-05-04 2015-05-26 Codexis, Inc. Biocatalysts for ezetimibe synthesis
US8759064B2 (en) 2010-05-14 2014-06-24 Codexis, Inc. Cellobiohydrolase variants
US20110294170A1 (en) 2010-05-28 2011-12-01 Codexis, Inc. Pentose Fermentation By a Recombinant Microorganism
EA022932B1 (ru) 2010-06-09 2016-03-31 Зимодженетикс, Инк. Димерные слитые белки vstm3 и связанные с ними композиции и способы
CA2798152A1 (en) 2010-06-17 2011-12-22 Dupont Nutrition Biosciences Aps Process
ES2755909T3 (es) 2010-07-12 2020-04-24 Celgene Corp Formas sólidas de la romidepsina y sus usos
US8993837B2 (en) 2010-08-13 2015-03-31 Pioneer Hi-Bred International, Inc Chimeric promoters and methods of use
US8932836B2 (en) 2010-08-16 2015-01-13 Codexis, Inc. Biocatalysts and methods for the synthesis of (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine
WO2012024662A2 (en) 2010-08-20 2012-02-23 Codexis, Inc. Expression constructs comprising fungal promoters
CN103080306B (zh) 2010-08-20 2015-02-18 科德克希思公司 糖苷水解酶61家族蛋白在纤维素加工中的用途
US8859502B2 (en) 2010-09-13 2014-10-14 Celgene Corporation Therapy for MLL-rearranged leukemia
EP2620428B1 (de) 2010-09-24 2019-05-22 Wave Life Sciences Ltd. Asymmetrische hilfsgruppe
CA2811789A1 (en) 2010-10-06 2012-04-12 Bp Corporation North America Inc. Variant cbh i polypeptides with reduced product inhibition
CN103189516A (zh) 2010-11-02 2013-07-03 科德克希思公司 用于产生可发酵糖类的组合物和方法
BR112013010813A2 (pt) 2010-11-02 2016-07-12 Codexis Inc células fúngicas, misturadas de enzimas, métodos para geração de glicose e meio de fermentação.
WO2012078312A2 (en) 2010-11-12 2012-06-14 Gen9, Inc. Methods and devices for nucleic acids synthesis
EP4039363A1 (de) 2010-11-12 2022-08-10 Gen9, Inc. Proteinanordnungen und verfahren zu ihrer herstellung und verwendung
DK2649187T3 (da) 2010-12-08 2018-01-29 Codexis Inc Biokatalysatorer og fremgangsmåder til syntesen af armodafinil
WO2012088159A2 (en) 2010-12-21 2012-06-28 Codexis, Inc. Endoglucanase variants
WO2012106579A1 (en) 2011-02-03 2012-08-09 Bristol-Myers Squibb Company Amino acid dehydrogenase and its use in preparing amino acids from keto acids
US20140051146A1 (en) 2011-02-23 2014-02-20 Dupont Nutrition Biosciences Aps Method
WO2012114234A1 (en) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Process
WO2012114232A1 (en) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Process
US8663962B2 (en) 2011-03-30 2014-03-04 Codexis, Inc. Pentose fermentation by a recombinant microorganism
CN105907846A (zh) 2011-04-07 2016-08-31 美艾利尔圣地亚哥有限公司 重组聚合酶扩增混合物的监测
JP6027092B2 (ja) 2011-04-08 2016-11-16 ダニスコ・ユーエス・インク 組成物
EP2697662B1 (de) 2011-04-13 2018-06-06 Codexis, Inc. Biokatalytisches verfahren zur herstellung von eslicarbazepin und analoga davon
BR112013033614A2 (pt) 2011-06-30 2018-05-29 Codexis Inc fermentação de pentose por microorganismo recombinante
US9605019B2 (en) 2011-07-19 2017-03-28 Wave Life Sciences Ltd. Methods for the synthesis of functionalized nucleic acids
US9322007B2 (en) 2011-07-22 2016-04-26 The California Institute Of Technology Stable fungal Cel6 enzyme variants
WO2013028701A1 (en) 2011-08-22 2013-02-28 Codexis, Inc. Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity
BR112014003740A2 (pt) 2011-08-23 2018-08-14 Codexis Inc variantes da celobiohidrolase
CN104066844B (zh) 2011-08-26 2016-12-07 Gen9股份有限公司 用于核酸的高保真组装的组合物和方法
CN103998461B (zh) 2011-09-08 2018-02-02 科德克希思公司 用于合成取代的内酰胺的生物催化剂和方法
EP2760997A4 (de) 2011-09-30 2015-02-11 Codexis Inc Pilzproteasen
CN103906835A (zh) 2011-10-25 2014-07-02 先锋国际良种公司 改变植物细胞壁组成以改善生物燃料生产和青贮饲料可消化性的方法
WO2013063702A1 (en) 2011-11-04 2013-05-10 Zymeworks Inc. Stable heterodimeric antibody design with mutations in the fc domain
WO2013074650A1 (en) 2011-11-18 2013-05-23 Codexis, Inc. Biocatalysts for the preparation of hydroxy substituted carbamates
EP2794893A4 (de) 2011-12-20 2015-08-12 Codexis Inc Verfahren zur herstellung von gesättigten fettalkoholen aus manipulierten mikroorganismen
BR112014015030A2 (pt) 2011-12-20 2017-06-13 Codexis Inc variantes da endoflucanase 1b (eg1b)
WO2013104659A2 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process
WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
US20150050658A1 (en) * 2012-03-15 2015-02-19 Codexix, Inc. Gene shuffling methods
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
HUE036985T2 (hu) 2012-03-23 2018-08-28 Codexis Inc Biokatalizátor és eljárások triptamin és triptamin-analógok származékainak szintetizálására
WO2013159055A1 (en) 2012-04-20 2013-10-24 Codexis, Inc. Production of fatty alcohols from engineered microorganisms
EP4001427A1 (de) 2012-04-24 2022-05-25 Gen9, Inc. Verfahren zum sortieren von nukleinsäuren und zur multiplexierten, vorbereitenden in-vitro-klonung
WO2013160372A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
WO2013160374A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling
CA2872096A1 (en) 2012-05-04 2013-11-07 E. I. Du Pont De Nemours And Company Compositions and methods comprising sequences having meganuclease activity
SG11201407295YA (en) 2012-05-08 2014-12-30 Codexis Inc Biocatalysts and methods for hydroxylation of chemical compounds
WO2013170050A1 (en) 2012-05-11 2013-11-14 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
US10045499B2 (en) 2012-05-24 2018-08-14 Iowa State University Research Foundation, Inc. Arabidopsis nonhost resistance gene(s) and use thereof to engineer disease resistant plants
WO2013188313A2 (en) 2012-06-11 2013-12-19 Codexis, Inc. Fungal xylanases and xylosidases
CN104685116A (zh) 2012-06-25 2015-06-03 Gen9股份有限公司 用于核酸组装和高通量测序的方法
BR112015000784A8 (pt) 2012-07-13 2018-04-03 Wave Life Sciences Japan Grupo auxiliar assimétrico
RU2677639C2 (ru) 2012-07-13 2019-01-18 Шин Ниппон Биомедикал Лэбораториз, Лтд. Хиральный адъювант нуклеиновой кислоты
PL2872147T3 (pl) 2012-07-13 2023-09-25 Wave Life Sciences Ltd. Sposób wytwarzania chiralnych oligonukleotydów
WO2014015278A1 (en) 2012-07-20 2014-01-23 Codexis, Inc. Production of fatty alcohols from engineered microorganisms
US9506087B2 (en) 2012-08-07 2016-11-29 Codexis, Inc. Glucose and xylose co-utilization in E. coli
ES2693461T3 (es) 2012-08-16 2018-12-11 Bangladesh Jute Research Institute Enzimas que degradan celulosa y/o hemicelulosas de Macrophomina phaseolina y usos de las mismas
ES2674922T3 (es) 2012-08-16 2018-07-05 Bangladesh Jute Research Institute Enzimas degradantes de pectina a partir de macrophomina phaseolina y usos de las mismas
AU2013202506B2 (en) 2012-09-07 2015-06-18 Celgene Corporation Resistance biomarkers for hdac inhibitors
EP2909230B1 (de) 2012-10-19 2019-05-22 Danisco US Inc. Verfahren zur stabilisierung von biometrischen membranen
US9663532B2 (en) 2012-10-29 2017-05-30 University Of Rochester Artemisinin derivatives, methods for their preparation and their use as antimalarial agents
AU2013202507B9 (en) 2012-11-14 2015-08-13 Celgene Corporation Inhibition of drug resistant cancer cells
CA2891482A1 (en) 2012-11-20 2014-05-30 Codexis, Inc. Pentose fermentation by a recombinant microorganism
WO2014088693A1 (en) * 2012-12-06 2014-06-12 Agilent Technologies, Inc. Molecular fabrication
US9512447B2 (en) 2012-12-14 2016-12-06 Codexis, Inc. Modified native beta-ketoacyl-ACP synthases and engineered microorganisms
CN105008528A (zh) 2012-12-21 2015-10-28 科德克希思公司 用于合成手性胺的工程化的生物催化剂和方法
HUE044110T2 (hu) 2013-01-18 2019-09-30 Codexis Inc Módosított biokatalizátorok, amelyek alkalmasak karbapeném szintézisére
NZ710299A (en) 2013-01-31 2020-01-31 Codexis Inc Methods, systems, and software for identifying bio-molecules with interacting components
US20140243356A1 (en) 2013-02-07 2014-08-28 The Regents Of The University Of California Use of translational profiling to identify target molecules for therapeutic treatment
CN105164263B (zh) 2013-02-28 2022-07-01 科德克希思公司 用于工业生物催化的工程化转氨酶多肽
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
BR112015023286A2 (pt) 2013-03-14 2018-03-06 Arzeda Corp polipeptídeo recombinante com atividade da dicamba descarboxilase, construto de polinucleotídeo, célula, método de produção de uma célula hospedeira compreendendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade da dicamba descarboxilase, método para descarboxilar dicamba, um derivado de dicamba ou um metabolito de dicamba, método para a detecção de um polipeptideo e método para a detecção da presença de um polinucleotideo que codifica um polipeptideo tendo atividade da dicamba descarboxilase
WO2014153234A1 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
WO2014159850A2 (en) 2013-03-14 2014-10-02 Codexis, Inc. Low-phosphate repressible promoter
US20160024516A1 (en) 2013-03-15 2016-01-28 Pioneer Hi-Bred International, Inc. Modulation of ACC Deaminase Expression
CN114525272A (zh) * 2013-03-15 2022-05-24 Gen9股份有限公司 多重核酸合成的组合物和方法
WO2014150914A2 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
LT2986722T (lt) 2013-04-18 2019-09-10 Codexis, Inc. Rekombinantiniai fenilalanino amonio liazės polipeptidai
WO2014202089A2 (en) 2013-06-18 2014-12-24 Roskilde Universitet Variants of anti-freeze polypeptides
CN105408492A (zh) 2013-07-25 2016-03-16 巴斯夫酶有限责任公司 肌醇六磷酸酶
US10006045B2 (en) 2013-08-16 2018-06-26 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3175967A1 (en) 2013-09-13 2015-03-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3049973B1 (de) 2013-09-27 2018-08-08 Codexis, Inc. Automatisiertes screening von enzymvarianten
CA2929664C (en) 2013-11-13 2023-01-24 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
EP3083936B1 (de) 2013-12-19 2018-07-04 Danisco US Inc. Verwendung von hydrophobinen zur erhöhung des gastransfers in einem aeroben fermentationsprozess
EP3087088B1 (de) 2013-12-23 2022-05-25 University Of Rochester Verfahren und zusammensetzungen zur ribosomalen synthese von makrocyclischen peptiden
NZ630311A (en) 2013-12-27 2016-03-31 Celgene Corp Romidepsin formulations and uses thereof
WO2015108046A1 (ja) 2014-01-15 2015-07-23 株式会社新日本科学 抗アレルギー作用を有するキラル核酸アジュバンド及び抗アレルギー剤
EP3095459A4 (de) 2014-01-15 2017-08-23 Shin Nippon Biomedical Laboratories, Ltd. Chirales nukleinsäureadjuvans mit antitumorwirkung und antitumormittel
US10144933B2 (en) 2014-01-15 2018-12-04 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having immunity induction activity, and immunity induction activator
CN113278617A (zh) 2014-01-16 2021-08-20 波涛生命科学有限公司 手性设计
UA120608C2 (uk) 2014-02-07 2020-01-10 Піонір Хай-Бред Інтернешнл, Інк. Очищений поліпептид ptip-83 та спосіб його застосування
WO2015120270A1 (en) 2014-02-07 2015-08-13 Pioneer Hi Bred International, Inc. Insecticidal proteins and methods for their use
US20170121741A1 (en) 2014-04-01 2017-05-04 Dupont Nutrition Biosciences Aps Method for increasing crude palm oil yields
US9605252B2 (en) 2014-04-16 2017-03-28 Codexis, Inc. Engineered tyrosine ammonia lyase
DK3167052T3 (da) 2014-07-09 2020-02-10 Codexis Inc P450-bm3-varianter med forbedret aktivitet
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
CN113584015A (zh) 2014-08-27 2021-11-02 新英格兰生物实验室公司 合成子的形成
US10045987B2 (en) 2014-08-28 2018-08-14 Codexis, Inc. Imidazoyl anilide derivatives and methods of use
WO2016033296A1 (en) 2014-08-28 2016-03-03 Codexis, Inc. N-substituted 4-aminoquinazoline derivatives and methods of use
BR112017007932A2 (pt) 2014-10-16 2018-01-23 Du Pont proteínas inseticidas e métodos para uso das mesmas
JP6719463B2 (ja) 2014-11-25 2020-07-15 コデクシス， インコーポレイテッド ケトン化合物およびアミン化合物の還元的アミノ化のための操作されたイミンレダクターゼおよび方法
EP4234699A1 (de) 2014-12-22 2023-08-30 Codexis, Inc. Menschliche alpha-galactosidasevarianten
WO2016130412A1 (en) 2015-02-10 2016-08-18 Codexis, Inc. Ketoreductase polypeptides for the synthesis of chiral compounds
BR112017019419B1 (pt) 2015-03-11 2022-10-11 E. I. Du Pont De Nemours And Company Construto de dna, método de obtenção de uma planta transgênica e método para controlar uma população de praga de verme da raiz do milho
WO2016164583A2 (en) 2015-04-07 2016-10-13 Novozymes A/S Methods for selecting enzymes having enhanced activity
CN107864658A (zh) 2015-04-07 2018-03-30 诺维信公司 用于选择具有脂肪酶活性的酶的方法
EP3292136B1 (de) 2015-05-07 2021-02-17 Codexis, Inc. Penicillin-g-acylasen
EP3294757B1 (de) 2015-05-13 2023-12-27 Synlogic Operating Company, Inc. Manipulierte bakterien zur behandlung einer krankheit oder einer störung
US9920102B2 (en) 2015-05-15 2018-03-20 Albert Einstein College Of Medicine, Inc. Fusion tags for protein expression
CN108064233B (zh) 2015-05-19 2022-07-15 先锋国际良种公司 杀昆虫蛋白及其使用方法
JP6867302B2 (ja) * 2015-05-21 2021-04-28 フル スペクトラム ジェネティクス， インコーポレイテッド タンパク質の特徴を改善する方法
US11291693B2 (en) 2015-06-25 2022-04-05 Synlogic Operating Company, Inc. Bacteria engineered to treat metabolic diseases
CA2991195C (en) 2015-07-07 2021-06-29 Codexis, Inc. Novel p450-bm3 variants with improved activity
CN109475096B (zh) 2015-08-06 2022-08-23 先锋国际良种公司 植物来源的杀昆虫蛋白及其使用方法
WO2017044424A1 (en) 2015-09-08 2017-03-16 Theripion, Inc. Apoa-1 fusion polypeptides and related compositions and methods
GB201516348D0 (en) 2015-09-15 2015-10-28 Labgenius Ltd Compositions and methods for polynucleotide assembly
WO2017100376A2 (en) 2015-12-07 2017-06-15 Zymergen, Inc. Promoters from corynebacterium glutamicum
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US20180325119A1 (en) 2015-12-18 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2017123592A1 (en) 2016-01-11 2017-07-20 Synlogic, Inc. Bacteria engineered to treat disorders associated with bile salts
WO2017123676A1 (en) 2016-01-11 2017-07-20 Synlogic, Inc. Recombinant bacteria engineered to treat diseases and disorders associated with amino acid metabolism and methods of use thereof
WO2017123418A1 (en) 2016-01-11 2017-07-20 Synlogic, Inc. Bacteria engineered to treat metabolic diseases
WO2017139708A1 (en) 2016-02-10 2017-08-17 Synlogic, Inc. Bacteria engineered to treat nonalcoholic steatohepatitis (nash)
CN109312333A (zh) 2016-04-07 2019-02-05 诺维信公司 选择具有蛋白酶活性的酶的方法
EA201892293A1 (ru) 2016-05-04 2019-04-30 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
AU2017260355B2 (en) 2016-05-05 2023-03-30 Codexis, Inc. Penicillin-G acylases
CN109715817B (zh) 2016-06-09 2022-12-09 科德克希思公司 用于化合物的羟基化的生物催化剂和方法
CN109715182A (zh) 2016-06-15 2019-05-03 科德克希思公司 工程化β-葡糖苷酶和葡糖基化方法
EP3472186A1 (de) 2016-06-17 2019-04-24 National Hellenic Research Foundation Systeme zur rekombinanten proteinproduktion
WO2018005793A1 (en) 2016-06-30 2018-01-04 Zymergen Inc. Methods for generating a glucose permease library and uses thereof
KR102345899B1 (ko) 2016-06-30 2021-12-31 지머젠 인코포레이티드 박테리아 헤모글로빈 라이브러리를 생성하는 방법 및 이의 용도
US11155829B2 (en) 2016-07-01 2021-10-26 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
EP3515926B1 (de) 2016-08-26 2023-10-25 Codexis, Inc. Manipulierte iminreduktasen und verfahren zur reduktiven aminierung von keton- und aminverbindungen
BR112019008800A2 (pt) 2016-11-01 2019-07-16 Pioneer Hi Bred Int polipeptídeo inseticida, composição inseticida, polinucleotídeo recombinante, construto de dna, célula de planta ou planta transgênica, método para inibir o crescimento ou exterminar uma população de praga de inseto agrícola, método para inibir o crescimento ou exterminar uma praga de inseto, método para controlar infestação de inseto lepidoptera e/ou coleoptera em uma planta transgênica e fornecer gerenciamento de resistência de inseto e uso de pelo menos um polipeptídeo inseticida
WO2018136163A2 (en) 2016-12-09 2018-07-26 Theripion, Inc. Tandem apoa-1 fusion polypeptides
BR112019012339A2 (pt) 2016-12-14 2019-11-26 Pioneer Hi Bred Int polipeptídeo inseticida recombinante, composição, construto de dna, célula hospedeira, planta transgênica, método para inibir o crescimento ou extermínio de uma praga de inseto ou população de praga, polipeptídeo ipd093 quimérico e proteína de fusão
CA3046226A1 (en) 2016-12-22 2018-06-28 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
AU2018205718B2 (en) 2017-01-05 2023-09-28 Codexis, Inc. Penicillin-G acylases
MX2019009233A (es) 2017-02-03 2019-09-19 Codexis Inc Glucosiltransferasas modificadas con ingenieria genetica y metodos de glucosilacion de glucosido de esteviol.
CA3052794A1 (en) 2017-02-08 2018-08-16 Pioneer Hi-Bred International, Inc. Insecticidal combinations of plant derived insecticidal proteins and methods for their use
NZ755628A (en) 2017-02-13 2023-09-29 Codexis Inc Engineered phenylalanine ammonia lyase polypeptides
EP3615058A4 (de) 2017-04-27 2021-06-02 Codexis, Inc. Ketoreduktase-polypeptide und polynukleotide
CN110914415A (zh) 2017-05-08 2020-03-24 科德克希思公司 工程化连接酶变体
WO2018209301A1 (en) 2017-05-11 2018-11-15 Cytodyn Inc. Methods for treating or preventing graft-versus-host disease involving the administration of anti-ccr5 receptor agents
RU2019140646A (ru) 2017-05-11 2021-06-11 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
EP3485013B1 (de) * 2017-06-06 2021-03-24 Zymergen, Inc. Genomische htp-engineering-plattform zur verbesserung von escherichia coli
KR20200015606A (ko) * 2017-06-06 2020-02-12 지머젠 인코포레이티드 사카로폴리스포라 스피노사를 개량하기 위한 고 처리량(htp) 게놈 공학 플랫폼
US11359183B2 (en) 2017-06-14 2022-06-14 Codexis, Inc. Engineered transaminase polypeptides for industrial biocatalysis
US11643642B2 (en) 2017-06-27 2023-05-09 Codexis, Inc. Penicillin-g acylases
EP3645711A4 (de) 2017-06-30 2021-04-21 Codexis, Inc. T7-rna-polymerasevarianten
KR20200023454A (ko) 2017-06-30 2020-03-04 코덱시스, 인코포레이티드 T7 rna 폴리머라제 변이체
US11634400B2 (en) 2017-08-19 2023-04-25 University Of Rochester Micheliolide derivatives, methods for their preparation and their use as anticancer and antiinflammatory agents
SG11202003094PA (en) 2017-11-07 2020-05-28 Codexis Inc Transglutaminase variants
MX2020006222A (es) 2017-12-13 2020-08-31 Codexis Inc Polipeptidos de carboxiesterasa para acoplamiento de amidas.
BR112020023407A2 (pt) 2018-05-17 2021-02-09 Bp Corporation North America Inc. produção de ácido 2-ceto-3-desoxi-d-glucônico em fungos filamentosos
CA3102968A1 (en) 2018-06-12 2019-12-19 Codexis, Inc. Engineered tyrosine ammonia lyase
JP2021530982A (ja) 2018-07-09 2021-11-18 コデクシス， インコーポレイテッド 操作されたガラクトースオキシダーゼ改変体酵素
KR20210031933A (ko) 2018-07-09 2021-03-23 코덱시스, 인코포레이티드 조작된 포스포펜토뮤타제 변이체 효소
CA3103718A1 (en) 2018-07-09 2020-01-16 Codexis, Inc. Engineered purine nucleoside phosphorylase variant enzymes
SG11202012136YA (en) 2018-07-09 2021-01-28 Codexis Inc Engineered pantothenate kinase variant enzymes
KR20210032417A (ko) 2018-07-09 2021-03-24 코덱시스, 인코포레이티드 조작된 데옥시리보스-포스페이트 알돌라아제
EP3820833A4 (de) 2018-07-12 2022-08-03 Codexis, Inc. Manipulierte phenylalanin-ammoniak-lyase-polypeptide
BR112021001661A8 (pt) 2018-07-30 2022-08-09 Codexis Inc Glicosiltransferase modificada, polinucleotídeo modificado, vetor, célula hospedeira, métodos para produzir pelo menos uma glicosiltransferase modificada, para produzir pelo menos uma variante de sacarose sintase, para glicosilação de um substrato, para produzir rebaudiosídeo m, rebaudiosídeo a e/ou rebaudiosídeo i e rebaudiosídeo d, composição, sacarose sintase modificada, pelo menos um rebaudiosídeo, rebaudiosídeo m, rebaudiosídeo a, rebaudiosídeo i, e, rebaudiosídeo d.
WO2020049157A1 (en) 2018-09-07 2020-03-12 Basf Plant Science Company Gmbh Improved method for the production of high levels of pufa in plants
US20210317467A1 (en) 2018-09-07 2021-10-14 Basf Plant Science Copmany Gmbh Improved method for the production of high levels of pufa in plants
EP3847262A1 (de) 2018-09-07 2021-07-14 Basf Plant Science Company GmbH Verbessertes verfahren zur herstellung von hohen pufa-spiegeln in pflanzen
US11060075B2 (en) 2018-10-29 2021-07-13 Codexis, Inc. Engineered DNA polymerase variants
AU2019397401A1 (en) 2018-12-14 2021-06-17 Codexis, Inc. Engineered tyrosine ammonia lyase
AU2019403323A1 (en) 2018-12-20 2021-07-01 Codexis, Inc. Human alpha-galactosidase variants
WO2020198731A2 (en) 2019-03-28 2020-10-01 Danisco Us Inc Engineered antibodies
JP2022542524A (ja) 2019-08-02 2022-10-04 サイトダイン インコーポレイテッド 抗ｃｃｒ５受容体薬剤の投与を含むがんを処置または予防する方法
MX2022007714A (es) 2019-12-20 2022-07-19 Basf Se Disminucion de la toxicidad de terpenos y aumento de la posible produccion en microorganismos.
US11045546B1 (en) 2020-03-30 2021-06-29 Cytodyn Inc. Methods of treating coronavirus infection
JP2023521772A (ja) 2020-04-10 2023-05-25 コデクシス， インコーポレイテッド 操作されたトランスアミナーゼポリペプチド
CN115516102A (zh) 2020-06-04 2022-12-23 艾瑟拜奥尼克斯公司 合成的檀香烯合酶
CA3186978A1 (en) 2020-07-14 2022-01-20 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
JP2023539634A (ja) 2020-08-28 2023-09-15 コデクシス， インコーポレイテッド 操作されたアミラーゼバリアント
US20220186231A1 (en) 2020-12-11 2022-06-16 Willow Biosciences, Inc. Recombinant acyl activating enzyme (aae) genes for enhanced biosynthesis of cannabinoids and cannabinoid precursors
WO2022133289A2 (en) 2020-12-18 2022-06-23 Codexis, Inc. Engineered uridine phosphorylase variant enzymes
EP4015626A1 (de) 2020-12-18 2022-06-22 Isobionics B.V. Enzyme und verfahren zur fermentativen herstellung von monoterpenestern
US20240101982A1 (en) 2021-02-19 2024-03-28 Theripion, Inc. Paraoxonase fusion polypeptides and related compositions and methods
CA3214972A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered adenylate kinase variant enzymes
WO2022212832A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered cyclic gmp-amp synthase (cgas) variant enzymes
CN117120599A (zh) 2021-04-02 2023-11-24 科德克希思公司 工程化鸟苷酸激酶变体酶
US11913036B2 (en) 2021-04-02 2024-02-27 Codexis, Inc. Engineered acetate kinase variant enzymes
CA3227215A1 (en) 2021-07-30 2023-02-02 Trish Choudhary Recombinant prenyltransferase polypeptides engineered for enhanced biosynthesis of cannabinoids
CN117795087A (zh) 2021-08-02 2024-03-29 巴斯夫欧洲公司 用紫罗兰亚基乙烷合酶进行的芳香化合物的新颖生产
CA3227236A1 (en) 2021-08-19 2023-02-23 Trish Choudhary Recombinant olivetolic acid cyclase polypeptides engineered for enhanced biosynthesis of cannabinoids
WO2023069921A1 (en) 2021-10-19 2023-04-27 Epimeron Usa, Inc. Recombinant thca synthase polypeptides engineered for enhanced biosynthesis of cannabinoids
CN114015672B (zh) * 2021-12-06 2022-05-31 江南大学 一种Pfu DNA聚合酶
WO2023173084A1 (en) 2022-03-11 2023-09-14 University Of Rochester Cyclopeptibodies and uses thereof
WO2023245171A1 (en) 2022-06-17 2023-12-21 Synlogic Operating Company, Inc. Bacteria engineered to treat diseases associated with bile acid metabolism and methods of use thereof
WO2023245168A1 (en) 2022-06-17 2023-12-21 Synlogic Operating Company, Inc. Bacteria engineered to treat diseases associated with bile acid metabolism and methods of use thereof
WO2023250478A1 (en) 2022-06-23 2023-12-28 Synlogic Operating Company, Inc. Recombinant bacteria engineered to treat diseases associated with methionine metabolism and methods of use thereof
WO2024040020A1 (en) 2022-08-15 2024-02-22 Absci Corporation Quantitative affinity activity specific cell enrichment

Family Cites Families (119)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994379A (en) 1983-03-09 1991-02-19 Cetus Corporation Modified signal peptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5422266A (en) * 1984-12-31 1995-06-06 University Of Georgia Research Foundation, Inc. Recombinant DNA vectors capable of expressing apoaequorin
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DE3590766C2 (de) * 1985-03-30 1991-01-10 Marc Genf/Geneve Ch Ballivet
US4959312A (en) * 1985-05-31 1990-09-25 The University Of Tennessee Research Corporation Full spectrum mutagenesis
EP0208491B1 (de) * 1985-07-03 1993-08-25 Genencor International, Inc. Hybride Polypeptide und Verfahren zu deren Herstellung
US5866363A (en) 1985-08-28 1999-02-02 Pieczenik; George Method and means for sorting and identifying biological information
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
DK311186D0 (da) * 1986-06-30 1986-06-30 Novo Industri As Enzymer
US5824469A (en) 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
US5763192A (en) * 1986-11-20 1998-06-09 Ixsys, Incorporated Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
FR2641793B1 (fr) 1988-12-26 1993-10-01 Setratech Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases
DD282028A5 (de) 1989-02-16 1990-08-29 Akad Wissenschaften Ddr Verfahren zur herstellung einer thermostabilen, hybriden bacillus-beta-1,3-1,4-glukanase
US5106727A (en) 1989-04-27 1992-04-21 Life Technologies, Inc. Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5556750A (en) * 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
CA2016842A1 (en) * 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
CA2016841C (en) 1989-05-16 1999-09-21 William D. Huse A method for producing polymers having a preselected activity
IT1231157B (it) * 1989-07-20 1991-11-19 Crc Ricerca Chim Nuovi geni ibridi funzionali di bacillus thuringiensis ottenuti mediante ricombinazione in vivo.
US5574205A (en) 1989-07-25 1996-11-12 Cell Genesys Homologous recombination for universal donor cells and chimeric mammalian hosts
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
WO1991006570A1 (en) * 1989-10-25 1991-05-16 The University Of Melbourne HYBRID Fc RECEPTOR MOLECULES
US5071743A (en) * 1989-10-27 1991-12-10 Her Majesty The Queen In Right Of Canada, As Represented By The National Research Council Of Canada Process for conducting site-directed mutagenesis
DE3936258C1 (de) * 1989-10-31 1991-04-25 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften Ev, 3400 Goettingen, De
AU6886991A (en) * 1989-11-08 1991-06-13 United States of America, as represented by the Secretary, U.S. Department of Commerce, The A method of synthesizing double-stranded dna molecules
US5242937A (en) 1990-03-19 1993-09-07 Research Corporation Technologies, Inc. Topically active ocular thiadiazole sulfonamide carbonic anhydrase inhibitors
ATE126535T1 (de) * 1990-04-05 1995-09-15 Roberto Crea ''walk-through''-mutagenese.
EP0528881A4 (en) * 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US5877402A (en) 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5851813A (en) * 1990-07-12 1998-12-22 President And Fellows Of Harvard College Primate lentivirus antigenic compositions
ATE176002T1 (de) * 1990-07-24 1999-02-15 Hoffmann La Roche Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen
EP0544809B1 (de) 1990-08-24 1998-12-16 Ixsys, Inc. Verfahren zur herstellung von oligonukleotiden mit regellosen codonen
US5264563A (en) 1990-08-24 1993-11-23 Ixsys Inc. Process for synthesizing oligonucleotides with random codons
IL99553A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing oligonucleotides linked to expression elements,a kit for the preparation of vectors useful for the expression of a diverse population of random peptides and methods utilizing the same
US5770434A (en) 1990-09-28 1998-06-23 Ixsys Incorporated Soluble peptides having constrained, secondary conformation in solution and method of making same
US5871974A (en) 1990-09-28 1999-02-16 Ixsys Inc. Surface expression libraries of heteromeric receptors
US5698426A (en) 1990-09-28 1997-12-16 Ixsys, Incorporated Surface expression libraries of heteromeric receptors
US5858725A (en) * 1990-10-10 1999-01-12 Glaxo Wellcome Inc. Preparation of chimaeric antibodies using the recombinant PCR strategy
US5234824A (en) * 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
US5489523A (en) * 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
EP0563296B1 (de) 1990-12-20 1999-03-17 Ixsys, Inc. Optimierung von bindenden proteinen
US5098437A (en) 1991-02-13 1992-03-24 Pfizer Hospital Products Group, Inc. Acetabular cup positioning insert
US5795747A (en) * 1991-04-16 1998-08-18 Evotec Biosystems Gmbh Process for manufacturing new biopolymers
DE4112440C1 (de) 1991-04-16 1992-10-22 Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De
US5514568A (en) * 1991-04-26 1996-05-07 Eli Lilly And Company Enzymatic inverse polymerase chain reaction
US5512463A (en) * 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
EP0519338B1 (de) 1991-06-20 1996-08-28 F. Hoffmann-La Roche Ag Verbesserte Methoden zur Nukleinsäure-Amplifizierung
WO1993000103A1 (en) * 1991-06-21 1993-01-07 The Wistar Institute Of Anatomy And Biology Chimeric envelope proteins for viral targeting
DE69232084T2 (de) * 1991-07-01 2002-05-02 Berlex Lab Neuartige methoden der mutagenese und reagentien dafür
US6071889A (en) 1991-07-08 2000-06-06 Neurospheres Holdings Ltd. In vivo genetic modification of growth factor-responsive neural precursor cells
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
GB9115364D0 (en) * 1991-07-16 1991-08-28 Wellcome Found Antibody
US5279952A (en) * 1991-08-09 1994-01-18 Board Of Regents, The University Of Texas System PCR-based strategy of constructing chimeric DNA molecules
DE69229477T2 (de) * 1991-09-23 1999-12-09 Cambridge Antibody Tech Methoden zur Herstellung humanisierter Antikörper
GB9125979D0 (en) * 1991-12-06 1992-02-05 Wellcome Found Antibody
AU674137B2 (en) * 1992-01-30 1996-12-12 Genzyme Limited Chiral synthesis with modified enzymes
US5773267A (en) * 1992-02-07 1998-06-30 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University D29 shuttle phasmids and uses thereof
GB9202796D0 (en) * 1992-02-11 1992-03-25 Wellcome Found Antiviral antibody
US5843643A (en) 1992-02-21 1998-12-01 Ratner; Paul L. Site-specific transfection of eukaryotic cells using polypeptide-linked recombinant nucleic acid
WO1993018141A1 (en) * 1992-03-02 1993-09-16 Biogen, Inc. Thrombin receptor antagonists
CA2132306C (en) * 1992-03-16 2012-07-10 Jacob N. Wohlstadter Selection methods
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
WO1993025237A1 (en) * 1992-06-15 1993-12-23 City Of Hope Chimeric anti-cea antibody
EP0651808B1 (de) * 1992-07-10 2001-12-12 Energy Biosystems Corporation Für einen entschwefelungs-biokatalysator kodierende, rekombinante dna
US6107062A (en) * 1992-07-30 2000-08-22 Inpax, Inc. Antisense viruses and antisense-ribozyme viruses
FR2694754B1 (fr) * 1992-08-12 1994-09-16 Bio Merieux Fragments d'ADN de mycobactéries, amorces d'amplification, sondes d'hybridation, réactifs et procédé de détection de détection de mycobactéries.
CA2126967A1 (en) * 1992-11-04 1994-05-11 Anna M. Wu Novel antibody construct
JPH08505524A (ja) 1992-11-10 1996-06-18 イグジス， インコーポレイティッド 溶液中で拘束された二次コンホメーションを有する可溶性ペプチド、およびその製造方法
US5360728A (en) * 1992-12-01 1994-11-01 Woods Hole Oceanographic Institution (W.H.O.I.) Modified apoaequorin having increased bioluminescent activity
EP0672142B1 (de) * 1992-12-04 2001-02-28 Medical Research Council Multivalente und multispezifische bindungsproteine, deren herstellung und verwendung
US5380530A (en) 1992-12-29 1995-01-10 Whitehill Oral Technologies Oral care composition coated gum
IT1264712B1 (it) * 1993-07-13 1996-10-04 Eniricerche Spa Metodo di sintesi biologica di peptidi
US5498531A (en) 1993-09-10 1996-03-12 President And Fellows Of Harvard College Intron-mediated recombinant techniques and reagents
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
DE4343591A1 (de) * 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5521077A (en) * 1994-04-28 1996-05-28 The Leland Stanford Junior University Method of generating multiple protein variants and populations of protein variants prepared thereby
US5514588A (en) 1994-12-13 1996-05-07 Exxon Research And Engineering Company Surfactant-nutrients for bioremediation of hydrocarbon contaminated soils and water
US5629179A (en) * 1995-03-17 1997-05-13 Novagen, Inc. Method and kit for making CDNA library
DE19612631A1 (de) 1995-03-31 1996-10-02 Mazda Motor Multiplexdatenübermittlungssystem
HUP9801871A3 (en) * 1995-04-24 1999-12-28 Chromaxome Corp San Diego Methods for generating and screening novel metabolic pathways
US6030779A (en) 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US6168919B1 (en) 1996-07-17 2001-01-02 Diversa Corporation Screening methods for enzymes and enzyme kits
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6004788A (en) 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
JP4156666B2 (ja) * 1995-08-11 2008-09-24 ノボザイムス アクティーゼルスカブ ポリペプチド変異体を調整するための方法
US5962258A (en) 1995-08-23 1999-10-05 Diversa Corporation Carboxymethyl cellulase fromthermotoga maritima
US6051409A (en) * 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
US5756316A (en) 1995-11-02 1998-05-26 Genencor International, Inc. Molecular cloning by multimerization of plasmids
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
DK0873398T3 (da) * 1996-01-10 2005-08-15 Novozymes As Fremgangsmåde til in vivo produktion af et mutantbibliotek i celler
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US6093873A (en) 1996-08-19 2000-07-25 Institut National De La Sante Et De La Recherche Medicale Genetically engineered mice containing alterations in the gene encoding RXR
WO1998028416A1 (en) * 1996-12-20 1998-07-02 Novo Nordisk A/S In vivo recombination
JP4263248B2 (ja) * 1997-03-18 2009-05-13 ノボザイムス アクティーゼルスカブ Ｄｎａのシャッフリングによるライブラリーの作成方法
NZ337910A (en) * 1997-03-18 2001-10-26 Novo Nordisk As Shuffling of heterologous DNA sequences using conserved regions
PL335860A1 (en) * 1997-03-18 2000-05-22 Novo Nordisk As Method of in vitro constructing a dna library
US5948653A (en) * 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US6051049A (en) 1997-05-29 2000-04-18 Exothermic Distribution Corporation Utilization of strontium aluminate in steelmaking
US6087341A (en) 1998-02-12 2000-07-11 The Board Of Trustees Of The Leland Standford Junior University Introduction of nucleic acid into skin cells by topical application

Also Published As

Publication number Publication date
DE876509T1 (de) 2001-04-05
EP0911396B1 (de) 2001-09-19
US6117679A (en) 2000-09-12
EP1138763A2 (de) 2001-10-04
EP0911396A2 (de) 1999-04-28
US6413774B1 (en) 2002-07-02
EP0876509A1 (de) 1998-11-11
AU713952B2 (en) 1999-12-16
DK0876509T3 (da) 2002-02-18
US6372497B1 (en) 2002-04-16
ES2165124T3 (es) 2002-03-01
DE911396T1 (de) 2001-04-05
EP1138763A3 (de) 2002-02-27
CA2239099C (en) 2004-11-30
DE69615406T2 (de) 2002-06-20
CA2239099A1 (en) 1997-06-05
DE69615411D1 (de) 2001-10-25
DE69615411T2 (de) 2002-06-20
DK0911396T3 (da) 2002-02-18
US6323030B1 (en) 2001-11-27
ES2164929T3 (es) 2002-03-01
EP1103606A2 (de) 2001-05-30
JP2004187694A (ja) 2004-07-08
EP0876509B1 (de) 2001-09-19
US6180406B1 (en) 2001-01-30
ATE205877T1 (de) 2001-10-15
DE69615406T3 (de) 2010-05-20
AU1087397A (en) 1997-06-19
ES2164929T5 (es) 2010-04-12
ATE205878T1 (de) 2001-10-15
JP2000308490A (ja) 2000-11-07
WO1997020078A1 (en) 1997-06-05
US6291242B1 (en) 2001-09-18
EP1103606A3 (de) 2001-08-22
EP0876509A4 (de) 1999-04-28
EP0876509B2 (de) 2009-11-11
IL124675A0 (en) 1998-12-06
DK0876509T4 (da) 2010-04-19
JP4015193B2 (ja) 2007-11-28
US6573098B1 (en) 2003-06-03
JP2000500981A (ja) 2000-02-02
US6344356B1 (en) 2002-02-05
EP0911396A3 (de) 1999-05-06

Similar Documents

Publication Publication Date Title
ATE205877T1 (de) Verfahren zur herstellung von polynukleotiden mit gewünschten eigenschaften durch iterative selektion und rekombination
DE69133018D1 (de) Methoden zur zielrichtung von dna
WO2001088173A3 (en) Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics
DE69433487D1 (de) Methoden und zusammensetzungen zur effizienten nukleinsaeuresequenzierung
DE69128545D1 (de) Technik des genetischen fingerabdrucks mit dns-vervielfältigung
IL137868A0 (en) Oligonucleotide mediated nucleic acid recombination
DE69233458D1 (de) Nukleinsäuretypisierung durch polymeraseverlängerung von oligonukleotiden unter verwendung von terminator-mischungen
ATE118818T1 (de) Verfahren zur herstellung synthetischer oligonukleotide, die spezifisch an bestimmungsstellen auf duplex-dna-molekülen binden durch formen eines kolinearen triplex, die synthetischen oligonukleotide und verfahren zu ihrer herstellung.
ATE277200T1 (de) Verfahren zur herstellung von biopolymeren mit veränderten eigenschaften
US20090087834A1 (en) Method for characterising polynucleotides
ATE405676T1 (de) Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination
ATE310103T1 (de) Methode zur bestimmung von polynukleotidsequenzvariationen
DE69921594D1 (de) Methoden zur synthese von polynukleotiden durch ligation von multiplen oligomeren
WO2002046457A3 (de) Verfahren zur codierung von hybriddisierungssonden
WO2002050094A3 (en) Nucleic acid synthesis by primer extension on successive templates

Legal Events

Date Code Title Description
8363 Opposition against the patent
8366 Restricted maintained after opposition proceedings