SG11201407295YA - Biocatalysts and methods for hydroxylation of chemical compounds - Google Patents

Biocatalysts and methods for hydroxylation of chemical compounds

Info

Publication number
SG11201407295YA
SG11201407295YA SG11201407295YA SG11201407295YA SG11201407295YA SG 11201407295Y A SG11201407295Y A SG 11201407295YA SG 11201407295Y A SG11201407295Y A SG 11201407295YA SG 11201407295Y A SG11201407295Y A SG 11201407295YA SG 11201407295Y A SG11201407295Y A SG 11201407295YA
Authority
SG
Singapore
Prior art keywords
singapore
international
biocatalysts
methods
engineered proline
Prior art date
Application number
SG11201407295YA
Inventor
Haibin Chen
Yong Koy Bong
Fabien Cabirol
Anupam Gohel
Tao Li
Jeffrey C Moore
Martina Quintanar-Audelo
Hong Yang
Steven J Collier
Derek Smith
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of SG11201407295YA publication Critical patent/SG11201407295YA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C12P17/12—Nitrogen as only ring hetero atom containing a six-membered hetero ring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/24—Proline; Hydroxyproline; Histidine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11002—Procollagen-proline dioxygenase (1.14.11.2), i.e. proline-hydroxylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 14 November 2013 (14.11.2013) WIPOIPCT (10) International Publication Number WO 2013/169725 A3 (51) International Patent Classification: C12N 9/04 { 2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: (30) Priority Data: 61/644,135 8 May 2012 (08.05.2012) (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Drive, Redwood City, California 94063 (US). (74) Agents: WHITING, Adam, K. et al.; CODEXIS, INC., 200 Penobscot Drive, Redwood City, California 94063 (US). PCT/US2013/039874 (81) Designated States (unless otherwise indicated , for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. 7 May 2013 (07.05.2013) English English US (72) Inventors: CHEN, Haibin; 61 Science Park Rd, #03- 15/24 THe Galen, Singapore Science Parkll, Singapore 117525 (SG). BONG, Yong Koy; BLK 652C #14-406, Jurong West St 61, Singapore 643652 (SG). CABIROL, Fabien; Bergerstrasse 24, 40213 Dusseldorf (DE). GO- HEL, Anupam; Pinus Hijau IV, No. 12, Meadow green, Lippo-cikarang, Bekasi, 17550 (ID). LI, Tao; 2521 Spindehill Drive Apt 4, Cincinnati, Ohio 45230 (US). MOORE, Jeffrey C.; 367 Orenda Circle, Westfield, New Jersey 07090 (US). QUINTANAR-AUDELO, Martina; 6.GhimMoh Rd #14-200, Singapore 270006 (SG). YANG, Hong; 61 Science Park Rd, #03-15/24 The Galen, Singa­ pore Science Parkll, Singapore 117525 (SG). COLLIER, Steven, J.; 22 Burroughs Rd, Lexington, Massachusetts 02420 (US). SMITH, Derek; 12 Holland Avenue #10-31, 272012 Singapore, Singapore 117525 (SG). (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) (88) Date of publication of the international search report: 6 March 2014 CJ i> o\ i-H cn i-H o CJ (54) Title: BIOCATALYSTS AND METHODS FOR HYDROXYLATION OF CHEMICAL COMPOUNDS (57) Abstract: The present disclosure provides engineered proline hydroxylase polypeptides for the production ofhydroxylated com­ pounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hy­ droxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active phar­ maceutical agents. The present disclosure provides engineered proline hydroxylase biocatalysts, polynucleotides encoding the biocatalysts. methods of their preparation, and processes for preparing hydroxylated compounds using these engineered biocatalysts.
SG11201407295YA 2012-05-08 2013-05-07 Biocatalysts and methods for hydroxylation of chemical compounds SG11201407295YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261644135P 2012-05-08 2012-05-08
PCT/US2013/039874 WO2013169725A2 (en) 2012-05-08 2013-05-07 Biocatalysts and methods for hydroxylation of chemical compounds

Publications (1)

Publication Number Publication Date
SG11201407295YA true SG11201407295YA (en) 2014-12-30

Family

ID=49551430

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201407295YA SG11201407295YA (en) 2012-05-08 2013-05-07 Biocatalysts and methods for hydroxylation of chemical compounds

Country Status (8)

Country Link
US (6) US9790527B2 (en)
EP (1) EP2847327B1 (en)
CN (2) CN107699548B (en)
DK (1) DK2847327T3 (en)
ES (1) ES2712682T3 (en)
HU (1) HUE042605T2 (en)
SG (1) SG11201407295YA (en)
WO (1) WO2013169725A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2847327B1 (en) 2012-05-08 2018-12-26 Codexis, Inc. Biocatalysts and methods for hydroxylation of chemical compounds
WO2015098774A1 (en) * 2013-12-26 2015-07-02 株式会社カネカ Production method for optically active cyclic imino acid
CN117886738A (en) 2013-12-27 2024-04-16 株式会社Api Process for producing 5-hydroxypiperidine-2-carboxylic acid
EP3101123B1 (en) 2014-01-31 2019-12-11 API Corporation Pipecolinic acid position-4 hydroxylase and method for producing 4-hydroxyamino acid using same
EP3219805B1 (en) 2014-11-12 2024-01-17 API Corporation Method for manufacturing cis-5-hydroxy-l-pipecolic acid
CN107304430B (en) * 2016-04-19 2020-10-27 中国科学院微生物研究所 DNA molecules and methods for the preparation of trans-4-hydroxy-L-proline
US10184117B2 (en) 2016-06-09 2019-01-22 Codexis, Inc. Biocatalysts and methods for hydroxylation of chemical compounds
EP3536780A4 (en) * 2016-11-04 2020-06-17 Asymchem Laboratories (Tianjin) Co., Ltd. Proline hydroxylase and application thereof
CN106834244B (en) * 2016-11-04 2021-07-06 凯莱英医药集团（天津）股份有限公司 Proline hydroxylase and application thereof
JP7289836B2 (en) * 2017-11-23 2023-06-12 エフ． ホフマン－ラ ロシュ アーゲー Proline hydroxylase and associated uses, methods and products
EP4065704A4 (en) * 2019-11-26 2024-08-07 Codexis Inc Biocatalysts and methods for hydroxylation of chemical compounds
CN113499732B (en) * 2021-07-09 2022-07-26 山东科技大学 Fracturing fluid and preparation method and application thereof

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05130883A (en) 1991-11-13 1993-05-28 Kyowa Hakko Kogyo Co Ltd Production of trans-l-hydroxyproline
US5854040A (en) 1993-09-07 1998-12-29 Kyowa Hakko Kogyo Co., Ltd. Process for producing trans-4-hydroxy-L-proline
KR100356926B1 (en) 1993-09-07 2003-01-10 교와 핫꼬 고교 가부시끼가이샤 Process for producing trans-4-hydroxy-l-proline
US5962292A (en) 1993-09-07 1999-10-05 Kyowa Hakko Kogyo Co., Ltd. Process for producing cis-3-hydroxy-L-proline
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
ATE206460T1 (en) 1994-06-03 2001-10-15 Novo Nordisk Biotech Inc PURIFIED MYCELIOPTHHORA LACCASES AND NUCLEIC ACIDS CODING THEREOF
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
FI104465B (en) 1995-06-14 2000-02-15 Valio Oy Protein hydrolyzates for the treatment and prevention of allergies and their preparation and use
KR0160191B1 (en) 1995-08-12 1999-01-15 김광호 Method & apparatus for back light correction
DK1036198T3 (en) 1997-12-08 2013-01-02 California Inst Of Techn Method for Preparation of Polynucleotide and Polypeptide Sequences
JP4221100B2 (en) 1999-01-13 2009-02-12 エルピーダメモリ株式会社 Semiconductor device
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
EP1272967A2 (en) 2000-03-30 2003-01-08 Maxygen, Inc. In silico cross-over site selection
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US7629157B2 (en) 2003-08-11 2009-12-08 Codexis, Inc. Ketoreductase polypeptides and related polynucleotides
WO2009008908A2 (en) 2007-02-12 2009-01-15 Codexis, Inc. Structure-activity relationships
RS53052B (en) 2008-01-18 2014-04-30 Merck Sharp & Dohme Corp. Beta-lactamase inhibitors
FR2930553B1 (en) 2008-04-29 2010-05-21 Novexel AZABICYCLIC COMPOUNDS, THEIR PREPARATION AND THEIR USE AS MEDICAMENTS, IN PARTICULAR BETA-LACTAMASES INHIBITORS
US8541209B2 (en) * 2008-05-12 2013-09-24 Kyowa Hakko Bio Co., Ltd. L-proline cis-4-hydroxylase and use thereof to produce cis-4-hydroxy-L-proline
WO2009139635A1 (en) 2008-05-15 2009-11-19 Prosensa Technologies B.V. Polymyxin derivatives and uses thereof
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
CN102186972B (en) * 2008-08-29 2014-08-20 科德克希思公司 Ketoreductase polypeptides for the stereoselective production of (4s)-3[(5s)-5(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one
US20120053350A1 (en) 2009-04-30 2012-03-01 Ian Mangion Preparation of alkyl esters of n-protected oxo-azacycloalkylcarboxylic acids
EP2847327B1 (en) * 2012-05-08 2018-12-26 Codexis, Inc. Biocatalysts and methods for hydroxylation of chemical compounds

Also Published As

Publication number Publication date
US10370688B2 (en) 2019-08-06
EP2847327B1 (en) 2018-12-26
WO2013169725A3 (en) 2014-03-06
US9790527B2 (en) 2017-10-17
EP2847327A2 (en) 2015-03-18
ES2712682T3 (en) 2019-05-14
US20150118719A1 (en) 2015-04-30
US20230227875A1 (en) 2023-07-20
US10995349B2 (en) 2021-05-04
DK2847327T3 (en) 2019-03-11
CN107699548B (en) 2021-10-22
US20170121744A1 (en) 2017-05-04
US11549132B2 (en) 2023-01-10
WO2013169725A2 (en) 2013-11-14
US20190323046A1 (en) 2019-10-24
CN104428412A (en) 2015-03-18
CN104428412B (en) 2017-09-08
EP2847327A4 (en) 2015-11-18
US10731189B2 (en) 2020-08-04
US20210230654A1 (en) 2021-07-29
CN107699548A (en) 2018-02-16
US20200325510A1 (en) 2020-10-15
HUE042605T2 (en) 2019-07-29

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