ATE405676T1 - Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination - Google Patents
Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombinationInfo
- Publication number
- ATE405676T1 ATE405676T1 AT03717952T AT03717952T ATE405676T1 AT E405676 T1 ATE405676 T1 AT E405676T1 AT 03717952 T AT03717952 T AT 03717952T AT 03717952 T AT03717952 T AT 03717952T AT E405676 T1 ATE405676 T1 AT E405676T1
- Authority
- AT
- Austria
- Prior art keywords
- double stranded
- methods
- recombination
- complexes
- stranded dna
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US36372902P | 2002-03-11 | 2002-03-11 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE405676T1 true ATE405676T1 (de) | 2008-09-15 |
Family
ID=28041805
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03717952T ATE405676T1 (de) | 2002-03-11 | 2003-03-11 | Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination |
Country Status (8)
Country | Link |
---|---|
US (1) | US7176025B2 (de) |
EP (1) | EP1488001B1 (de) |
JP (1) | JP2005519621A (de) |
AT (1) | ATE405676T1 (de) |
AU (1) | AU2003222269A1 (de) |
CA (1) | CA2478875A1 (de) |
DE (1) | DE60323067D1 (de) |
WO (1) | WO2003078645A2 (de) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
JP2005503755A (ja) | 2000-12-13 | 2005-02-10 | ニューゲン テクノロジーズ, インコーポレイテッド | 核酸配列の複数コピーを作製するための方法および組成物、ならびにそれらを検出する方法 |
EP1390537B1 (de) * | 2001-03-09 | 2013-11-13 | Nugen Technologies, Inc. | Verfahren und zusammensetzungen zur amplifikation von rna-sequenzen |
AU2002252279B2 (en) | 2001-03-09 | 2005-05-12 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
AU2003222269A1 (en) | 2002-03-11 | 2003-09-29 | Nugen Technologies, Inc. | Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination |
JP4551216B2 (ja) * | 2002-05-17 | 2010-09-22 | ニューゲン テクノロジーズ, インコーポレイテッド | 核酸の断片化、標識および固定化の方法 |
WO2004092418A2 (en) | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US20050208538A1 (en) * | 2003-12-29 | 2005-09-22 | Nurith Kurn | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
WO2006045053A2 (en) * | 2004-10-20 | 2006-04-27 | Hitachi Chemical Company, Ltd. | Method for tailoring administration of drugs by quantitation of mrna |
WO2007030759A2 (en) * | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
CA2652052A1 (en) * | 2006-05-16 | 2007-11-29 | Nugen Technologies, Inc. | Nucleic acid separation and purification method based on reversible charge interactions |
CA2656315A1 (en) * | 2006-06-30 | 2008-01-10 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
US7846666B2 (en) | 2008-03-21 | 2010-12-07 | Nugen Technologies, Inc. | Methods of RNA amplification in the presence of DNA |
WO2011032053A1 (en) * | 2009-09-11 | 2011-03-17 | Nugen Technologies, Inc. | Compositions and methods for whole transcriptome analysis |
SG10201510189WA (en) | 2011-10-19 | 2016-01-28 | Nugen Technologies Inc | Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing |
GB2513793B (en) | 2012-01-26 | 2016-11-02 | Nugen Tech Inc | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
CN108018343B (zh) | 2012-05-10 | 2023-01-03 | 通用医疗公司 | 用于测定核苷酸序列的方法 |
JP6181751B2 (ja) | 2012-06-18 | 2017-08-16 | ニューゲン テクノロジーズ, インコーポレイテッド | 望まれない核酸配列のネガティブ選択のための組成物および方法 |
US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
WO2014028311A2 (en) * | 2012-08-15 | 2014-02-20 | President And Fellows Of Harvard College | Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing |
WO2014144092A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Sequential sequencing |
JP6525473B2 (ja) | 2013-11-13 | 2019-06-05 | ニューゲン テクノロジーズ, インコーポレイテッド | 複製物配列決定リードを同定するための組成物および方法 |
CA2938080A1 (en) | 2014-01-27 | 2015-07-30 | The General Hospital Corporation | Methods of preparing nucleic acids for sequencing |
US9745614B2 (en) | 2014-02-28 | 2017-08-29 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
CA2957633A1 (en) | 2014-08-06 | 2016-02-11 | Nugen Technologies, Inc. | Digital measurements from targeted sequencing |
US10683531B2 (en) | 2016-09-15 | 2020-06-16 | ArcherDX, Inc. | Methods of nucleic acid sample preparation for analysis of cell-free DNA |
EP3512947B1 (de) | 2016-09-15 | 2021-10-20 | ArcherDX, LLC | Verfahren für die zubereitung von nukleinsäureproben |
JP7127023B2 (ja) * | 2017-06-23 | 2022-08-29 | 栄研化学株式会社 | 核酸検出方法、核酸検出用プライマー及び核酸検出用キット |
US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
Family Cites Families (55)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4876187A (en) * | 1985-12-05 | 1989-10-24 | Meiogenics, Inc. | Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences |
US5403711A (en) * | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
JP2650159B2 (ja) * | 1988-02-24 | 1997-09-03 | アクゾ・ノベル・エヌ・ベー | 核酸増幅方法 |
AU623642B2 (en) | 1988-03-24 | 1992-05-21 | University Of Iowa Research Foundation, The | Catalytic hybridization systems for the detection of nucleic acid sequences based on their activity as cofactors in catalytic reactions in which a complementary labeled nucleic acid probe is cleaved |
US5130238A (en) * | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
EP0731174B1 (de) * | 1989-07-11 | 2004-11-17 | Gen-Probe Incorporated | Verfahren zur Amplifikation von Nukleinsäuresequenzen |
CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
CA2037349C (en) * | 1990-03-26 | 2008-06-17 | James G. Wetmur | Branch migration of nucleotides |
HU218095B (hu) * | 1990-05-01 | 2000-05-28 | Amgen Inc. | Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban |
US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
AU8997991A (en) | 1991-01-31 | 1992-08-06 | Becton Dickinson & Company | Exonuclease mediated strand displacement amplification |
US5169766A (en) * | 1991-06-14 | 1992-12-08 | Life Technologies, Inc. | Amplification of nucleic acid molecules |
US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
DE69332665T2 (de) * | 1992-03-11 | 2003-11-27 | Dana Farber Cancer Inst Inc | Methode um mrna zu klonieren |
US5262311A (en) * | 1992-03-11 | 1993-11-16 | Dana-Farber Cancer Institute, Inc. | Methods to clone polyA mRNA |
WO1993022461A1 (en) * | 1992-05-06 | 1993-11-11 | Gen-Probe Incorporated | Nucleic acid sequence amplification method, composition and kit |
WO1994003637A1 (en) * | 1992-07-31 | 1994-02-17 | Syntex (Usa) Inc. | Method for introducing defined sequences at the 3' end of polynucleotides |
US6027923A (en) * | 1993-07-23 | 2000-02-22 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
WO1995014108A1 (en) * | 1993-11-17 | 1995-05-26 | Amersham International Plc | Primer extension mass spectroscopy nucleic acid sequencing method |
CA2140081C (en) | 1994-01-13 | 2008-04-01 | Dean L. Engelhardt | Process, construct and conjugate for producing multiple nucleic acid copies |
US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6335160B1 (en) * | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
FR2724934B1 (fr) * | 1994-09-26 | 1997-01-24 | Bio Merieux | Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique |
US6280935B1 (en) * | 1994-10-13 | 2001-08-28 | Lynx Therapeutics, Inc. | Method of detecting the presence or absence of a plurality of target sequences using oligonucleotide tags |
US5665545A (en) * | 1994-11-28 | 1997-09-09 | Akzo Nobel N.V. | Terminal repeat amplification method |
US5830655A (en) * | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
US5700642A (en) * | 1995-05-22 | 1997-12-23 | Sri International | Oligonucleotide sizing using immobilized cleavable primers |
FR2737223B1 (fr) * | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
US6068829A (en) * | 1995-09-11 | 2000-05-30 | The Burnham Institute | Method of identifying molecules that home to a selected organ in vivo |
US5916779A (en) | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
US5962271A (en) * | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
EP2264045B1 (de) * | 1996-08-14 | 2015-10-21 | Life Technologies Corporation | Stabile Zusammensetzungen zur Amplifizierung und Sequenzierung von Nukleinsäuren |
US5958703A (en) * | 1996-12-03 | 1999-09-28 | Glaxo Group Limited | Use of modified tethers in screening compound libraries |
US20010000077A1 (en) * | 1998-02-03 | 2001-03-29 | Engelhardt Dean L. | Novel process, construct and conjugate for producing multiple nucleic acid copies |
US6087103A (en) * | 1998-03-04 | 2000-07-11 | Lifespan Biosciences, Inc. | Tagged ligand arrays for identifying target-ligand interactions |
GB9817055D0 (en) | 1998-08-05 | 1998-09-30 | Medical Res Council | Reverse transcription and amplification processes and primers therefore |
US6358712B1 (en) * | 1999-01-05 | 2002-03-19 | Trustee Of Boston University | Ordered gene assembly |
JP2002537762A (ja) | 1999-01-05 | 2002-11-12 | トラスティーズ オブ ボストン ユニバーシティ | 改良型核酸クローニング |
US6376246B1 (en) * | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US7074556B2 (en) | 1999-03-02 | 2006-07-11 | Invitrogen Corporation | cDNA synthesis improvements |
US6132997A (en) * | 1999-05-28 | 2000-10-17 | Agilent Technologies | Method for linear mRNA amplification |
DK1218542T3 (da) | 1999-09-13 | 2004-08-02 | Nugen Technologies Inc | Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser |
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6617442B1 (en) | 1999-09-30 | 2003-09-09 | Isis Pharmaceuticals, Inc. | Human Rnase H1 and oligonucleotide compositions thereof |
US6794138B1 (en) * | 1999-12-16 | 2004-09-21 | Affymetrix, Inc. | Methods of small sample amplification |
US7205129B1 (en) | 2000-02-28 | 2007-04-17 | Qiagen Gmbh | Method for reducing artifacts in nucleic acid amplification |
CN1498274A (zh) * | 2000-06-26 | 2004-05-19 | Ŧب������˾ | 基于转录的核酸扩增方法和组合物 |
EP1325118B1 (de) | 2000-10-05 | 2016-05-25 | Riken | Oligonucleotidlinker mit einem variablen kohesiven anteil und methode zur herstellung von polynucleotidbanken mittels dieser linker |
US20020127575A1 (en) | 2000-10-30 | 2002-09-12 | Glenn Hoke | Partially double-stranded nucleic acids, methods of making, and use thereof |
JP2005503755A (ja) * | 2000-12-13 | 2005-02-10 | ニューゲン テクノロジーズ, インコーポレイテッド | 核酸配列の複数コピーを作製するための方法および組成物、ならびにそれらを検出する方法 |
AU2002252279B2 (en) | 2001-03-09 | 2005-05-12 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences |
AU2003222269A1 (en) | 2002-03-11 | 2003-09-29 | Nugen Technologies, Inc. | Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination |
EP1490514A4 (de) | 2002-03-29 | 2008-12-17 | Nugen Technologies Inc | Durch isotherme einzelprimer-nukleinsäureamplifikation verbesserter nachweis und quantifizierung von analyten |
-
2003
- 2003-03-11 AU AU2003222269A patent/AU2003222269A1/en not_active Abandoned
- 2003-03-11 US US10/386,623 patent/US7176025B2/en not_active Expired - Fee Related
- 2003-03-11 CA CA002478875A patent/CA2478875A1/en not_active Abandoned
- 2003-03-11 JP JP2003576637A patent/JP2005519621A/ja active Pending
- 2003-03-11 WO PCT/US2003/007425 patent/WO2003078645A2/en active Application Filing
- 2003-03-11 EP EP03717952A patent/EP1488001B1/de not_active Expired - Lifetime
- 2003-03-11 AT AT03717952T patent/ATE405676T1/de not_active IP Right Cessation
- 2003-03-11 DE DE60323067T patent/DE60323067D1/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP1488001A2 (de) | 2004-12-22 |
WO2003078645A3 (en) | 2004-03-18 |
WO2003078645A2 (en) | 2003-09-25 |
AU2003222269A1 (en) | 2003-09-29 |
DE60323067D1 (de) | 2008-10-02 |
JP2005519621A (ja) | 2005-07-07 |
EP1488001B1 (de) | 2008-08-20 |
CA2478875A1 (en) | 2003-09-25 |
US20030215926A1 (en) | 2003-11-20 |
EP1488001A4 (de) | 2006-12-13 |
US7176025B2 (en) | 2007-02-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE405676T1 (de) | Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination | |
DE69433487D1 (de) | Methoden und zusammensetzungen zur effizienten nukleinsaeuresequenzierung | |
ATE528403T1 (de) | Verfahren zur herstellung von ungesättigten omega-3-fettsäuren in transgenen organismen | |
ATE205878T1 (de) | Verfahren zur herstellung von polynucleotiden mit erwünschten eigenschaften mittels wiederholter selektion und rekombination | |
DE60042969D1 (de) | Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren | |
ATE335086T1 (de) | Künstliche chromosomen, die die genetische information für die bildung von rekombinantem virus enthalten | |
DE69128545D1 (de) | Technik des genetischen fingerabdrucks mit dns-vervielfältigung | |
ATE243753T1 (de) | Mch4 und mch5, apoptotische proteasen, dafür kodierende nukleinsäure und verfahren zur verwendung | |
ATE392430T1 (de) | Verfahren zur herstellung von dipeptiden | |
DE60226182D1 (de) | Agarase und Gen dafür | |
WO2003006644A3 (de) | Lipase/acyltransferase | |
ATE329927T1 (de) | Rekombinante coryneformbakterie die glyceraldehyde-3-phosphate dehydrogenase -2 überexprimieren , und verfahren zur herstellung von l-lysine | |
ATE340258T1 (de) | Verfahren zur herstellung von nukleinsäuren bestehend aus stochastisch kombinierten anteilen von ausgangsnukleinsäuren | |
ATE516351T1 (de) | Verfahren zur molekularen evolution der proteinfunktion in vitro | |
HUP9904414A2 (hu) | A neuritin neurogén | |
EP1341909B8 (de) | Eine methode zur in vitro molekularen evolution der proteinfunktion | |
ATE519859T1 (de) | Verfahren zur herstellung von ausgewählten intermolekularen quervernetzungen in nukleinsäuren und ihre verwendungen | |
DE69937056D1 (de) | Verfahren zur transformation von tierzellen | |
DE60228867D1 (de) | Verfahren zur herstellung und identifizierung löslicher proteindomänen | |
DE69632963D1 (de) | Mch3, eine apoptosis-protease, kodierende nukleinsäuren und methoden zur verwendung | |
ATE329055T1 (de) | Methode zur erstellung von genexpressionsprofilen | |
WO2001083754A3 (en) | Cellular regulators of infectious agents and methods of use | |
AU2001276385A1 (en) | Nucleotide sequences which code for the lysr3 gene | |
WO2003066882A3 (en) | Method and apparatus for validating dna sequences without sequencing | |
DE60131320D1 (de) | Eine Nukleinsäure, die in menschlichen Tumorzellen aufreguliert wird, ein von dieser kodiertes Protein und ein Verfahren zur Tumordiagnose |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |