ATE439437T1 - Verbessertes klonierungsverfahren - Google Patents
Verbessertes klonierungsverfahrenInfo
- Publication number
- ATE439437T1 ATE439437T1 AT00915689T AT00915689T ATE439437T1 AT E439437 T1 ATE439437 T1 AT E439437T1 AT 00915689 T AT00915689 T AT 00915689T AT 00915689 T AT00915689 T AT 00915689T AT E439437 T1 ATE439437 T1 AT E439437T1
- Authority
- AT
- Austria
- Prior art keywords
- molecules
- product molecules
- ligation
- dna
- product
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Glass Compositions (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11490999P | 1999-01-05 | 1999-01-05 | |
US09/225,990 US6358712B1 (en) | 1999-01-05 | 1999-01-05 | Ordered gene assembly |
PCT/US2000/000189 WO2000040715A2 (en) | 1999-01-05 | 2000-01-05 | Improved nucleic acid cloning |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE439437T1 true ATE439437T1 (de) | 2009-08-15 |
Family
ID=26812654
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT00915689T ATE439437T1 (de) | 1999-01-05 | 2000-01-05 | Verbessertes klonierungsverfahren |
Country Status (8)
Country | Link |
---|---|
US (4) | US7579146B2 (de) |
EP (1) | EP1141275B1 (de) |
JP (3) | JP2002537762A (de) |
AT (1) | ATE439437T1 (de) |
AU (1) | AU774306B2 (de) |
CA (1) | CA2360011A1 (de) |
DE (1) | DE60042730D1 (de) |
WO (1) | WO2000040715A2 (de) |
Families Citing this family (36)
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US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
AU2001277014A1 (en) * | 2000-07-21 | 2002-02-05 | Trustees Of Boston University | Modular vector systems |
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WO2009076655A2 (en) * | 2007-12-13 | 2009-06-18 | University Of Washington | Synthetic enzymes derived from computational design |
US20090203531A1 (en) | 2008-02-12 | 2009-08-13 | Nurith Kurn | Method for Archiving and Clonal Expansion |
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WO2011056872A2 (en) | 2009-11-03 | 2011-05-12 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
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US9295965B2 (en) | 2010-11-12 | 2016-03-29 | Gen9, Inc. | Methods and devices for nucleic acid synthesis |
EP2637780B1 (de) | 2010-11-12 | 2022-02-09 | Gen9, Inc. | Proteinanordnungen und verfahren zu ihrer herstellung und verwendung |
US20130059296A1 (en) * | 2011-08-26 | 2013-03-07 | Gen9, Inc. | Compositions and Methods For High Fidelity Assembly of Nucleic Acids |
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
CA2871505C (en) | 2012-04-24 | 2021-10-12 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
US20150191719A1 (en) | 2012-06-25 | 2015-07-09 | Gen9, Inc. | Methods for Nucleic Acid Assembly and High Throughput Sequencing |
US10148285B1 (en) | 2012-07-25 | 2018-12-04 | Erich Schmitt | Abstraction and de-abstraction of a digital data stream |
US10795858B1 (en) | 2014-02-18 | 2020-10-06 | Erich Schmitt | Universal abstraction and de-abstraction of a digital data stream |
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2000
- 2000-01-05 JP JP2000592412A patent/JP2002537762A/ja not_active Ceased
- 2000-01-05 AU AU36921/00A patent/AU774306B2/en not_active Ceased
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- 2000-01-05 EP EP00915689A patent/EP1141275B1/de not_active Expired - Lifetime
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- 2000-01-05 DE DE60042730T patent/DE60042730D1/de not_active Expired - Lifetime
- 2000-01-05 CA CA002360011A patent/CA2360011A1/en not_active Abandoned
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Also Published As
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WO2000040715A9 (en) | 2002-08-29 |
US7579146B2 (en) | 2009-08-25 |
EP1141275B1 (de) | 2009-08-12 |
AU774306B2 (en) | 2004-06-24 |
DE60042730D1 (de) | 2009-09-24 |
US20050191623A1 (en) | 2005-09-01 |
EP1141275A2 (de) | 2001-10-10 |
WO2000040715A3 (en) | 2001-02-08 |
US20090317875A1 (en) | 2009-12-24 |
WO2000040715A2 (en) | 2000-07-13 |
US20070269858A1 (en) | 2007-11-22 |
US20060105358A1 (en) | 2006-05-18 |
JP2011200257A (ja) | 2011-10-13 |
JP2002537762A (ja) | 2002-11-12 |
US7439021B2 (en) | 2008-10-21 |
AU3692100A (en) | 2000-07-24 |
CA2360011A1 (en) | 2000-07-13 |
JP2007075128A (ja) | 2007-03-29 |
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