WO2003030623A3 - System for manipulating nucleic acids - Google Patents

System for manipulating nucleic acids Download PDF

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Publication number
WO2003030623A3
WO2003030623A3 PCT/US2002/033023 US0233023W WO03030623A3 WO 2003030623 A3 WO2003030623 A3 WO 2003030623A3 US 0233023 W US0233023 W US 0233023W WO 03030623 A3 WO03030623 A3 WO 03030623A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
product molecules
ligation
dna
product
Prior art date
Application number
PCT/US2002/033023
Other languages
French (fr)
Other versions
WO2003030623A2 (en
Inventor
Kevin A Jarrell
Brian Turczyk
Original Assignee
Modular Genetics Inc
Kevin A Jarrell
Brian Turczyk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Modular Genetics Inc, Kevin A Jarrell, Brian Turczyk filed Critical Modular Genetics Inc
Priority to AU2002340234A priority Critical patent/AU2002340234A1/en
Publication of WO2003030623A2 publication Critical patent/WO2003030623A2/en
Publication of WO2003030623A3 publication Critical patent/WO2003030623A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides an improved system for linking nucleic acids to one another. In particular, the present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The product molecules need not be cleaved with restriction enzymes in order to undergo such ligation. In preferred embodiments of the invention, the DNA product molecules are produced through iterative DNA synthesis reactions, so that the product molecules are amplified products. The invention further provides methods for directed ligation of product molecules (i.e., for selective ligation of certain molecules within a collection of molecules), and also for methods of exon shuffling, in which multiple different product molecules are produced in a single ligation reaction. Preferred embodiments of the invention involve ligation of product molecules encoding functional protein domains, particularly domains naturally found in conserved gene families. The inventive DNA manipulation system is readily integrated with other nucleic acid manipulation systems, such as ribozyme-mediated systems, and also is susceptible to automation.
PCT/US2002/033023 2001-10-12 2002-10-15 System for manipulating nucleic acids WO2003030623A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002340234A AU2002340234A1 (en) 2001-10-12 2002-10-15 System for manipulating nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32924601P 2001-10-12 2001-10-12
US60/329,246 2001-10-12

Publications (2)

Publication Number Publication Date
WO2003030623A2 WO2003030623A2 (en) 2003-04-17
WO2003030623A3 true WO2003030623A3 (en) 2004-03-04

Family

ID=23284520

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/033023 WO2003030623A2 (en) 2001-10-12 2002-10-15 System for manipulating nucleic acids

Country Status (2)

Country Link
AU (1) AU2002340234A1 (en)
WO (1) WO2003030623A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4740470A (en) * 1974-11-04 1988-04-26 The Board Of Trustees Of The Leland Stanford, Jr. University Biologically functional molecular chimeras
US5487993A (en) * 1990-09-27 1996-01-30 Invitrogen Corporation Direct cloning of PCR amplified nucleic acids

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4740470A (en) * 1974-11-04 1988-04-26 The Board Of Trustees Of The Leland Stanford, Jr. University Biologically functional molecular chimeras
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683195B1 (en) * 1986-01-30 1990-11-27 Cetus Corp
US5487993A (en) * 1990-09-27 1996-01-30 Invitrogen Corporation Direct cloning of PCR amplified nucleic acids

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
XIN WEN ET AL.: "Assembly of linear functional expression elements with DNA fragments digested with asymmetric restriction endonucleases", BIOTECHNOL. LETT., vol. 25, no. 11, 2003, pages 901 - 904, XP002973545 *
YANG ET AL.: "Amplification, cloning and sequence analyses of the VH and VK genes of an anti-human CD3 monoclonal antibody", CHINESE BIOCHEMICAL JOURNAL, vol. 10, no. 2, April 1994 (1994-04-01), pages 213 - 217, XP002973546 *

Also Published As

Publication number Publication date
WO2003030623A2 (en) 2003-04-17
AU2002340234A1 (en) 2003-04-22

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