IL127624A0 - Method and kits for preparing multicomponent nucleic acid constructs - Google Patents
Method and kits for preparing multicomponent nucleic acid constructsInfo
- Publication number
- IL127624A0 IL127624A0 IL12762497A IL12762497A IL127624A0 IL 127624 A0 IL127624 A0 IL 127624A0 IL 12762497 A IL12762497 A IL 12762497A IL 12762497 A IL12762497 A IL 12762497A IL 127624 A0 IL127624 A0 IL 127624A0
- Authority
- IL
- Israel
- Prior art keywords
- kits
- nucleic acid
- acid constructs
- multicomponent nucleic
- preparing multicomponent
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US1986996P | 1996-06-17 | 1996-06-17 | |
PCT/US1997/010523 WO1997048716A1 (en) | 1996-06-17 | 1997-06-16 | Method and kits for preparing multicomponent nucleic acid constructs |
Publications (1)
Publication Number | Publication Date |
---|---|
IL127624A0 true IL127624A0 (en) | 1999-10-28 |
Family
ID=21795483
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL12762497A IL127624A0 (en) | 1996-06-17 | 1997-06-16 | Method and kits for preparing multicomponent nucleic acid constructs |
Country Status (7)
Country | Link |
---|---|
US (2) | US6277632B1 (en) |
EP (1) | EP0915903A4 (en) |
JP (1) | JP2000512852A (en) |
AU (1) | AU3399797A (en) |
CA (1) | CA2258570A1 (en) |
IL (1) | IL127624A0 (en) |
WO (1) | WO1997048716A1 (en) |
Families Citing this family (56)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5766891A (en) | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
US7119250B2 (en) * | 1997-06-03 | 2006-10-10 | The University Of Chicago | Plant centromere compositions |
US7193128B2 (en) | 1997-06-03 | 2007-03-20 | Chromatin, Inc. | Methods for generating or increasing revenues from crops |
US7235716B2 (en) | 1997-06-03 | 2007-06-26 | Chromatin, Inc. | Plant centromere compositions |
US7227057B2 (en) | 1997-06-03 | 2007-06-05 | Chromatin, Inc. | Plant centromere compositions |
US5942609A (en) * | 1998-11-12 | 1999-08-24 | The Porkin-Elmer Corporation | Ligation assembly and detection of polynucleotides on solid-support |
US20040005673A1 (en) * | 2001-06-29 | 2004-01-08 | Kevin Jarrell | System for manipulating nucleic acids |
DE60042730D1 (en) * | 1999-01-05 | 2009-09-24 | Univ Boston | IMPROVED CLONING PROCESS |
US7989202B1 (en) | 1999-03-18 | 2011-08-02 | The University Of Chicago | Plant centromere compositions |
AU2001243232A1 (en) | 2000-02-25 | 2001-09-03 | University Of South Carolina Research Foundation | Topoisomerase linker-mediated amplification methods |
US7435562B2 (en) * | 2000-07-21 | 2008-10-14 | Modular Genetics, Inc. | Modular vector systems |
US20030017552A1 (en) * | 2000-07-21 | 2003-01-23 | Jarrell Kevin A. | Modular vector systems |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
CA2421057A1 (en) | 2000-08-21 | 2002-02-28 | Invitrogen Corporation | Methods and reagents for molecular cloning |
JP2004520047A (en) * | 2000-12-08 | 2004-07-08 | インヴィトロジェン コーポレーション | Compositions and methods for rapid production of recombinant nucleic acid molecules |
US20060008817A1 (en) | 2000-12-08 | 2006-01-12 | Invitrogen Corporation | Methods and compositions for generating recombinant nucleic acid molecules |
US20050260570A1 (en) * | 2001-05-29 | 2005-11-24 | Mao Jen-I | Sequencing by proxy |
US20040209239A1 (en) * | 2001-06-06 | 2004-10-21 | Paul Lieberman | Methods and compositions fir identifying episomal dna virus infection treatment |
US20040265805A1 (en) * | 2002-05-02 | 2004-12-30 | Monsanto Technology, L.L.C. | Method for cloning large DNA |
JP4510626B2 (en) * | 2002-08-21 | 2010-07-28 | エポック・バイオサイエンシーズ・インコーポレイテッド | Abasic site endonuclease assay |
US7563600B2 (en) | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
AU2003279089A1 (en) * | 2002-09-30 | 2004-04-19 | Parallele Bioscience, Inc. | Polynucleotide synthesis and labeling by kinetic sampling ligation |
US7561919B2 (en) * | 2002-11-14 | 2009-07-14 | Brainsgate Ltd. | SPG stimulation via the greater palatine canal |
EP1697534B1 (en) | 2003-12-01 | 2010-06-02 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
WO2005083096A1 (en) * | 2004-02-23 | 2005-09-09 | Chromatin, Inc. | Plants modified with mini-chromosomes |
US20050266447A1 (en) * | 2004-04-19 | 2005-12-01 | Pioneer Hi-Bred International, Inc. | Method for identifying activators of gene transcription |
US20070122817A1 (en) * | 2005-02-28 | 2007-05-31 | George Church | Methods for assembly of high fidelity synthetic polynucleotides |
AU2005295351A1 (en) * | 2004-10-18 | 2006-04-27 | Codon Devices, Inc. | Methods for assembly of high fidelity synthetic polynucleotides |
US20060141626A1 (en) * | 2004-10-27 | 2006-06-29 | Monsanto Technology Llc | Non-random method of gene shuffling |
JP2008526259A (en) * | 2005-01-13 | 2008-07-24 | コドン デバイシズ インコーポレイテッド | Compositions and methods for protein design |
US20060281113A1 (en) * | 2005-05-18 | 2006-12-14 | George Church | Accessible polynucleotide libraries and methods of use thereof |
EP1902126A1 (en) * | 2005-07-12 | 2008-03-26 | Codon Devices, Inc | Compositions and methods for biocatalytic engineering |
WO2007008951A1 (en) * | 2005-07-12 | 2007-01-18 | Codon Devices, Inc. | Compositions and methods for design of non-immunogenic proteins |
CA2621874C (en) * | 2005-09-08 | 2014-12-16 | Chromatin Inc. | Plants modified with mini-chromosomes |
US20070231805A1 (en) * | 2006-03-31 | 2007-10-04 | Baynes Brian M | Nucleic acid assembly optimization using clamped mismatch binding proteins |
US20090087840A1 (en) * | 2006-05-19 | 2009-04-02 | Codon Devices, Inc. | Combined extension and ligation for nucleic acid assembly |
US8053191B2 (en) * | 2006-08-31 | 2011-11-08 | Westend Asset Clearinghouse Company, Llc | Iterative nucleic acid assembly using activation of vector-encoded traits |
US8614089B2 (en) * | 2007-03-15 | 2013-12-24 | Chromatin, Inc. | Centromere sequences and minichromosomes |
US8637435B2 (en) * | 2007-11-16 | 2014-01-28 | Merck Sharp & Dohme Corp. | Eukaryotic cell display systems |
WO2009111183A1 (en) * | 2008-03-03 | 2009-09-11 | Glycofi, Inc. | Surface display of recombinant proteins in lower eukaryotes |
WO2011011693A1 (en) | 2009-07-23 | 2011-01-27 | Chromatin, Inc. | Sorghum centromere sequences and minichromosomes |
US10207240B2 (en) | 2009-11-03 | 2019-02-19 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
US9216414B2 (en) | 2009-11-25 | 2015-12-22 | Gen9, Inc. | Microfluidic devices and methods for gene synthesis |
US9217144B2 (en) | 2010-01-07 | 2015-12-22 | Gen9, Inc. | Assembly of high fidelity polynucleotides |
EP3705573A1 (en) | 2010-11-12 | 2020-09-09 | Gen9, Inc. | Methods and devices for nucleic acids synthesis |
WO2012064975A1 (en) | 2010-11-12 | 2012-05-18 | Gen9, Inc. | Protein arrays and methods of using and making the same |
ES2737957T3 (en) | 2011-08-26 | 2020-01-17 | Gen9 Inc | Compositions and methods for high fidelity nucleic acid assembly |
AR083981A1 (en) * | 2011-11-24 | 2013-04-10 | Consejo Nac Invest Cient Tec | NITROGEN FIXING RECOMBINANT BACTERIA CEPA, INOCULATE THAT CONTAINS IT AND APPLICATION METHODS |
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
AU2013251701A1 (en) | 2012-04-24 | 2014-10-30 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
US20150191719A1 (en) | 2012-06-25 | 2015-07-09 | Gen9, Inc. | Methods for Nucleic Acid Assembly and High Throughput Sequencing |
JP6745599B2 (en) | 2012-12-06 | 2020-08-26 | アジレント・テクノロジーズ・インクAgilent Technologies, Inc. | Preparation of molecule |
US9617522B2 (en) | 2013-09-05 | 2017-04-11 | Massachusetts Institute Of Technology | Tuning bacteriophage host range |
WO2017015348A1 (en) * | 2015-07-20 | 2017-01-26 | The Regents Of The University Of California | Sequence-independent nucleic acid assembly |
AU2017370751B2 (en) * | 2016-12-09 | 2023-11-09 | Ultivue, Inc. | Improved methods for multiplex imaging using labeled nucleic acid imaging agents |
KR20190111036A (en) | 2016-12-23 | 2019-10-01 | 비스테라, 인크. | Binding Polypeptides and Methods for Making the Same |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5128256A (en) | 1987-01-12 | 1992-07-07 | Stratagene | DNA cloning vectors with in vivo excisable plasmids |
US4987073A (en) | 1989-06-12 | 1991-01-22 | Akzo N.V. | Bacteriophage cloning system for the construction of directional DNA libraries |
WO1992018632A1 (en) * | 1991-04-12 | 1992-10-29 | Stratagene | Polycos vectors |
US5962255A (en) * | 1992-03-24 | 1999-10-05 | Cambridge Antibody Technology Limited | Methods for producing recombinant vectors |
US5137814A (en) | 1991-06-14 | 1992-08-11 | Life Technologies, Inc. | Use of exo-sample nucleotides in gene cloning |
US5470955A (en) * | 1993-02-02 | 1995-11-28 | Dartmouth College | Antibodies which specifically bind mcl-1 polypeptide |
US5426039A (en) | 1993-09-08 | 1995-06-20 | Bio-Rad Laboratories, Inc. | Direct molecular cloning of primer extended DNA containing an alkane diol |
US6495318B2 (en) * | 1996-06-17 | 2002-12-17 | Vectorobjects, Llc | Method and kits for preparing multicomponent nucleic acid constructs |
-
1997
- 1997-06-16 WO PCT/US1997/010523 patent/WO1997048716A1/en not_active Application Discontinuation
- 1997-06-16 AU AU33997/97A patent/AU3399797A/en not_active Abandoned
- 1997-06-16 CA CA002258570A patent/CA2258570A1/en not_active Abandoned
- 1997-06-16 EP EP97930083A patent/EP0915903A4/en not_active Withdrawn
- 1997-06-16 JP JP10503272A patent/JP2000512852A/en active Pending
- 1997-06-16 IL IL12762497A patent/IL127624A0/en unknown
- 1997-06-16 US US08/877,034 patent/US6277632B1/en not_active Expired - Lifetime
-
2006
- 2006-11-30 US US11/607,812 patent/US7485426B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP0915903A1 (en) | 1999-05-19 |
EP0915903A4 (en) | 2002-09-04 |
US6277632B1 (en) | 2001-08-21 |
US20080032399A1 (en) | 2008-02-07 |
AU3399797A (en) | 1998-01-07 |
CA2258570A1 (en) | 1997-12-24 |
JP2000512852A (en) | 2000-10-03 |
WO1997048716A1 (en) | 1997-12-24 |
US7485426B2 (en) | 2009-02-03 |
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