AU2001243232A1 - Topoisomerase linker-mediated amplification methods - Google Patents
Topoisomerase linker-mediated amplification methodsInfo
- Publication number
- AU2001243232A1 AU2001243232A1 AU2001243232A AU4323201A AU2001243232A1 AU 2001243232 A1 AU2001243232 A1 AU 2001243232A1 AU 2001243232 A AU2001243232 A AU 2001243232A AU 4323201 A AU4323201 A AU 4323201A AU 2001243232 A1 AU2001243232 A1 AU 2001243232A1
- Authority
- AU
- Australia
- Prior art keywords
- topoisomerase
- linker
- amplification methods
- mediated amplification
- mediated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18485800P | 2000-02-25 | 2000-02-25 | |
| US60184858 | 2000-02-25 | ||
| PCT/US2001/005745 WO2001062943A1 (en) | 2000-02-25 | 2001-02-23 | Topoisomerase linker-mediated amplification methods |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2001243232A1 true AU2001243232A1 (en) | 2001-09-03 |
Family
ID=22678649
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001243232A Abandoned AU2001243232A1 (en) | 2000-02-25 | 2001-02-23 | Topoisomerase linker-mediated amplification methods |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7078501B2 (en) |
| EP (1) | EP1261724A4 (en) |
| JP (1) | JP2003524422A (en) |
| AU (1) | AU2001243232A1 (en) |
| WO (1) | WO2001062943A1 (en) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5766891A (en) * | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
| DE69827745T2 (en) | 1997-06-12 | 2005-11-10 | Sloan-Kettering Institute For Cancer Research | COVALENT BINDING OF DNA TO RNA STRANDS CATALYSED BY VACCINIA TOPOISOMERASE |
| US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| JP5043277B2 (en) | 2000-08-21 | 2012-10-10 | ライフ テクノロジーズ コーポレーション | Molecular cloning methods and reagents used |
| WO2002061034A2 (en) * | 2000-12-08 | 2002-08-08 | Invitrogen Corporation | Compositions and methods for rapidly generating recombinant nucleic acid molecules |
| US20060008817A1 (en) * | 2000-12-08 | 2006-01-12 | Invitrogen Corporation | Methods and compositions for generating recombinant nucleic acid molecules |
| EP2287341B1 (en) | 2003-12-01 | 2013-02-13 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
| US20060063184A1 (en) * | 2004-09-09 | 2006-03-23 | Felix Carolyn A | Compositions and methods for the detection of DNA topoisomerase II complexes with DNA |
| CN104350067A (en) | 2012-04-10 | 2015-02-11 | 牛津纳米孔技术公司 | Mutant lysenin pores |
| KR102168813B1 (en) | 2013-03-08 | 2020-10-22 | 옥스포드 나노포어 테크놀로지즈 리미티드 | Enzyme stalling method |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| JP6749243B2 (en) * | 2014-01-22 | 2020-09-02 | オックスフォード ナノポール テクノロジーズ リミテッド | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| CN106574300A (en) | 2014-05-02 | 2017-04-19 | 牛津纳米孔技术公司 | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| US11274333B2 (en) * | 2015-05-29 | 2022-03-15 | Molecular Cloning Laboratories (MCLAB) LLC | Compositions and methods for preparing sequencing libraries |
| WO2016201111A1 (en) * | 2015-06-09 | 2016-12-15 | Centrillion Technology Holdings Corporation | Methods for sequencing nucleic acids |
| CA3016245A1 (en) | 2016-03-02 | 2017-09-08 | Oxford Nanopore Technologies Limited | Mutant csgg pores and their use in polynucleotide detection and characterisation |
| WO2017174990A1 (en) | 2016-04-06 | 2017-10-12 | Oxford Nanopore Technologies Limited | Mutant pore |
| WO2019002893A1 (en) | 2017-06-30 | 2019-01-03 | Vib Vzw | Novel protein pores |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4661450A (en) | 1983-05-03 | 1987-04-28 | Molecular Genetics Research And Development Limited Partnership | Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides |
| WO1985004898A1 (en) | 1984-04-26 | 1985-11-07 | The Australian National University | An improved method of cloning double-stranded rna, and its use in the production of viral gene clones |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| CA2005252C (en) | 1988-12-13 | 2001-07-17 | Nahum Sonenberg | Isolation of full-length cdna clones and capped mrna |
| JP2994010B2 (en) | 1989-10-31 | 1999-12-27 | 財団法人相模中央化学研究所 | Cloning vector plasmid, vector primer derived from the same plasmid and preparation of cdna bank using the same primer |
| CA2037349C (en) | 1990-03-26 | 2008-06-17 | James G. Wetmur | Branch migration of nucleotides |
| US5162209A (en) * | 1991-01-18 | 1992-11-10 | Beth Israel Hospital Association | Synthesis of full-length, double-stranded dna from a single-stranded linear dna template |
| JP3337748B2 (en) | 1992-09-25 | 2002-10-21 | 財団法人神奈川科学技術アカデミー | Method for synthesizing full-length cDNA, method for producing intermediate thereof, and method for producing recombinant vector containing full-length cDNA |
| DE4319468C1 (en) | 1993-06-11 | 1994-11-17 | Mueller Manfred W | Method for producing a deoxyribonucleic acid (DNA) strand (cDNA strand) complementary to a ribonucleic acid (RNA) molecule, and the use of the method for the analysis of RNA molecules |
| US5766891A (en) * | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
| FR2733762B1 (en) | 1995-05-02 | 1997-08-01 | Genset Sa | METHOD FOR THE SPECIFIC COUPLING OF THE HAIR OF THE 5 'END OF A RNAM FRAGMENT AND PREPARATION OF RNAM AND COMPLETE DNA |
| US6238884B1 (en) | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
| US6537776B1 (en) | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
| US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| US6013440A (en) * | 1996-03-11 | 2000-01-11 | Affymetrix, Inc. | Nucleic acid affinity columns |
| JP3346976B2 (en) * | 1996-03-18 | 2002-11-18 | 理化学研究所 | How to make a full-length cDNA library |
| CA2258570A1 (en) | 1996-06-17 | 1997-12-24 | Biodynamics Associates | Method and kits for preparing multicomponent nucleic acid constructs |
| US6495318B2 (en) | 1996-06-17 | 2002-12-17 | Vectorobjects, Llc | Method and kits for preparing multicomponent nucleic acid constructs |
| US5746997A (en) * | 1996-10-02 | 1998-05-05 | Epoch Pharmaceuticals, Inc. | Radiohalogenation of oligonucleotides via trialkylstannylaryl conjugates |
| JP3441899B2 (en) | 1996-11-01 | 2003-09-02 | 理化学研究所 | How to make a full-length cDNA library |
| US5942422A (en) | 1996-11-14 | 1999-08-24 | The Trustees Of Columbia University In The City Of New York | Method for generating a directed, recombinant fusion nucleic acid |
| US5851808A (en) * | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
| US5981182A (en) * | 1997-03-13 | 1999-11-09 | Albert Einstein College Of Medicine Of Yeshiva University | Vector constructs for the selection and identification of open reading frames |
| WO1998055502A1 (en) | 1997-06-04 | 1998-12-10 | Smithkline Beecham Corporation | METHODS FOR RAPID CLONING OF FULL LENGTH cDNAs |
| DE69827745T2 (en) * | 1997-06-12 | 2005-11-10 | Sloan-Kettering Institute For Cancer Research | COVALENT BINDING OF DNA TO RNA STRANDS CATALYSED BY VACCINIA TOPOISOMERASE |
| US6140086A (en) | 1997-08-15 | 2000-10-31 | Fox; Donna K. | Methods and compositions for cloning nucleic acid molecules |
| JP4304348B2 (en) | 1997-09-22 | 2009-07-29 | 独立行政法人理化学研究所 | DNA isolation method |
| US6413776B1 (en) | 1998-06-12 | 2002-07-02 | Galapagos Geonomics N.V. | High throughput screening of gene function using adenoviral libraries for functional genomics applications |
| WO2000012687A1 (en) | 1998-08-28 | 2000-03-09 | Invitrogen Corporation | System for the rapid manipulation of nucleic acid sequences |
| AU3397800A (en) | 1999-03-19 | 2000-10-09 | Invitrogen Corporation | Methods of obtaining full-length nucleic acid sequences using (e. coli) topoisomerase iii and its homologs |
| JP2002539800A (en) | 1999-03-24 | 2002-11-26 | ジーン セラピー システムズ インコーポレーテッド | Method for producing transcriptionally active DNA fragment |
| JP2004503207A (en) | 2000-02-25 | 2004-02-05 | ストラタジーン | Methods for ligating nucleic acids and molecular cloning |
| US20020025561A1 (en) | 2000-04-17 | 2002-02-28 | Hodgson Clague Pitman | Vectors for gene-self-assembly |
| US20020068290A1 (en) | 2000-05-31 | 2002-06-06 | Timur Yarovinsky | Topoisomerase activated oligonucleotide adaptors and uses therefor |
| JP5043277B2 (en) | 2000-08-21 | 2012-10-10 | ライフ テクノロジーズ コーポレーション | Molecular cloning methods and reagents used |
| WO2002061034A2 (en) | 2000-12-08 | 2002-08-08 | Invitrogen Corporation | Compositions and methods for rapidly generating recombinant nucleic acid molecules |
-
2001
- 2001-02-23 JP JP2001561753A patent/JP2003524422A/en active Pending
- 2001-02-23 WO PCT/US2001/005745 patent/WO2001062943A1/en not_active Application Discontinuation
- 2001-02-23 EP EP01916175A patent/EP1261724A4/en not_active Withdrawn
- 2001-02-23 AU AU2001243232A patent/AU2001243232A1/en not_active Abandoned
- 2001-02-23 US US09/792,875 patent/US7078501B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US20010044137A1 (en) | 2001-11-22 |
| EP1261724A1 (en) | 2002-12-04 |
| JP2003524422A (en) | 2003-08-19 |
| EP1261724A4 (en) | 2004-08-18 |
| WO2001062943A1 (en) | 2001-08-30 |
| US7078501B2 (en) | 2006-07-18 |
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