DE60323067D1 - Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination - Google Patents

Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination

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Publication number
DE60323067D1
DE60323067D1 DE60323067T DE60323067T DE60323067D1 DE 60323067 D1 DE60323067 D1 DE 60323067D1 DE 60323067 T DE60323067 T DE 60323067T DE 60323067 T DE60323067 T DE 60323067T DE 60323067 D1 DE60323067 D1 DE 60323067D1
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DE
Germany
Prior art keywords
methods
strengthed
recombination
complexes
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60323067T
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English (en)
Inventor
Nurith Kurn
Martin Junhong Wang
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Nugen Technologies Inc
Original Assignee
Nugen Technologies Inc
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Publication of DE60323067D1 publication Critical patent/DE60323067D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE60323067T 2002-03-11 2003-03-11 Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination Expired - Lifetime DE60323067D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36372902P 2002-03-11 2002-03-11
PCT/US2003/007425 WO2003078645A2 (en) 2002-03-11 2003-03-11 Methods for generating double stranded dna comprising a 3’ single stranded portion and uses of these complexes for recombination

Publications (1)

Publication Number Publication Date
DE60323067D1 true DE60323067D1 (de) 2008-10-02

Family

ID=28041805

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60323067T Expired - Lifetime DE60323067D1 (de) 2002-03-11 2003-03-11 Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination

Country Status (8)

Country Link
US (1) US7176025B2 (de)
EP (1) EP1488001B1 (de)
JP (1) JP2005519621A (de)
AT (1) ATE405676T1 (de)
AU (1) AU2003222269A1 (de)
CA (1) CA2478875A1 (de)
DE (1) DE60323067D1 (de)
WO (1) WO2003078645A2 (de)

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WO2002072773A2 (en) 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
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WO2004092418A2 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
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US9957549B2 (en) 2012-06-18 2018-05-01 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014028311A2 (en) * 2012-08-15 2014-02-20 President And Fellows Of Harvard College Polynucleotide-binding domains as a means of cell labeling, cell organization and polymer sequencing
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WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
AU2015209079B2 (en) 2014-01-27 2021-07-15 Archerdx, Llc Methods of preparing nucleic acids for sequencing
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CN107075581B (zh) 2014-08-06 2022-03-18 纽亘技术公司 由靶向测序进行数字测量
US10704082B2 (en) 2016-09-15 2020-07-07 ArcherDX, Inc. Methods of nucleic acid sample preparation
EP3512965B1 (de) 2016-09-15 2024-03-13 ArcherDX, LLC Verfahren zur herstellung von nukleinsäuren zur analyse von zellfreier dna
WO2018235886A1 (ja) * 2017-06-23 2018-12-27 栄研化学株式会社 核酸検出方法、核酸検出用プライマー及び核酸検出用キット
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system

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Also Published As

Publication number Publication date
WO2003078645A3 (en) 2004-03-18
CA2478875A1 (en) 2003-09-25
EP1488001A4 (de) 2006-12-13
AU2003222269A1 (en) 2003-09-29
ATE405676T1 (de) 2008-09-15
WO2003078645A2 (en) 2003-09-25
US20030215926A1 (en) 2003-11-20
EP1488001B1 (de) 2008-08-20
US7176025B2 (en) 2007-02-13
EP1488001A2 (de) 2004-12-22
JP2005519621A (ja) 2005-07-07

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