ATE516351T1 - Verfahren zur molekularen evolution der proteinfunktion in vitro - Google Patents
Verfahren zur molekularen evolution der proteinfunktion in vitroInfo
- Publication number
- ATE516351T1 ATE516351T1 AT06808581T AT06808581T ATE516351T1 AT E516351 T1 ATE516351 T1 AT E516351T1 AT 06808581 T AT06808581 T AT 06808581T AT 06808581 T AT06808581 T AT 06808581T AT E516351 T1 ATE516351 T1 AT E516351T1
- Authority
- AT
- Austria
- Prior art keywords
- polynucleotide
- sequence
- parent
- population
- fragments
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 238000000338 in vitro Methods 0.000 title 1
- 230000004853 protein function Effects 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 abstract 13
- 239000002157 polynucleotide Substances 0.000 abstract 13
- 102000040430 polynucleotide Human genes 0.000 abstract 13
- 239000012634 fragment Substances 0.000 abstract 4
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 2
- 101710163270 Nuclease Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0523582A GB2432366B (en) | 2005-11-19 | 2005-11-19 | A method for in vitro molecular evolution of protein function |
PCT/GB2006/004294 WO2007057682A1 (en) | 2005-11-19 | 2006-11-17 | A method for in vitro molecular evolution of protein function |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE516351T1 true ATE516351T1 (de) | 2011-07-15 |
Family
ID=35580344
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT06808581T ATE516351T1 (de) | 2005-11-19 | 2006-11-17 | Verfahren zur molekularen evolution der proteinfunktion in vitro |
Country Status (12)
Country | Link |
---|---|
US (1) | US20090203572A1 (de) |
EP (1) | EP1948794B1 (de) |
JP (1) | JP5349051B2 (de) |
CN (1) | CN101351553B (de) |
AT (1) | ATE516351T1 (de) |
AU (1) | AU2006314310B2 (de) |
CA (1) | CA2630183C (de) |
DK (1) | DK1948794T3 (de) |
ES (1) | ES2395249T3 (de) |
GB (1) | GB2432366B (de) |
PT (1) | PT1948794E (de) |
WO (1) | WO2007057682A1 (de) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020086292A1 (en) | 2000-12-22 | 2002-07-04 | Shigeaki Harayama | Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules |
GB0607798D0 (en) | 2006-04-20 | 2006-05-31 | Alligator Bioscience Ab | Novel polypeptides and use thereof |
GB0708376D0 (en) | 2007-05-01 | 2007-06-06 | Alligator Bioscience Ab | Novel polypeptides and uses thereof |
GB0905790D0 (en) | 2009-04-03 | 2009-05-20 | Alligator Bioscience Ab | Novel polypeptides and use thereof |
GB0905503D0 (en) * | 2009-03-31 | 2009-05-13 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
GB201019086D0 (en) | 2010-11-11 | 2010-12-29 | Imp Innovations Ltd | Bacterial methods |
GB201311475D0 (en) | 2013-06-27 | 2013-08-14 | Alligator Bioscience Ab | Polypeptides |
GB201311487D0 (en) | 2013-06-27 | 2013-08-14 | Alligator Bioscience Ab | Bispecific molecules |
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US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
DE229046T1 (de) * | 1985-03-30 | 1987-12-17 | Marc Genf/Geneve Ballivet | Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren. |
US6492107B1 (en) * | 1986-11-20 | 2002-12-10 | Stuart Kauffman | Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique |
US4994368A (en) * | 1987-07-23 | 1991-02-19 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
US5043272A (en) * | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
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US6291165B1 (en) * | 1997-03-18 | 2001-09-18 | Novo Nordisk A/S | Shuffling of heterologous DNA sequences |
US6159688A (en) * | 1997-03-18 | 2000-12-12 | Novo Nordisk A/S | Methods of producing polynucleotide variants |
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US6958213B2 (en) * | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
US20020086292A1 (en) * | 2000-12-22 | 2002-07-04 | Shigeaki Harayama | Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules |
US20030036641A1 (en) * | 2001-01-31 | 2003-02-20 | Padgett Hal S. | Methods for homology-driven reassembly of nucleic acid sequences |
US20030129709A1 (en) * | 2001-11-02 | 2003-07-10 | Olga Makarova | Method for site-directed mutagenesis of nucleic acid molecules using a single primer |
KR20040088538A (ko) * | 2002-02-26 | 2004-10-16 | 이 아이 듀폰 디 네모아 앤드 캄파니 | 유전 인자의 재조합 방법 |
WO2003097834A2 (en) * | 2002-05-17 | 2003-11-27 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
-
2005
- 2005-11-19 GB GB0523582A patent/GB2432366B/en active Active
-
2006
- 2006-11-17 ES ES06808581T patent/ES2395249T3/es active Active
- 2006-11-17 EP EP06808581A patent/EP1948794B1/de not_active Not-in-force
- 2006-11-17 DK DK06808581.0T patent/DK1948794T3/da active
- 2006-11-17 CA CA2630183A patent/CA2630183C/en not_active Expired - Fee Related
- 2006-11-17 AT AT06808581T patent/ATE516351T1/de active
- 2006-11-17 PT PT06808581T patent/PT1948794E/pt unknown
- 2006-11-17 JP JP2008540691A patent/JP5349051B2/ja not_active Expired - Fee Related
- 2006-11-17 US US12/094,066 patent/US20090203572A1/en not_active Abandoned
- 2006-11-17 CN CN2006800501278A patent/CN101351553B/zh not_active Expired - Fee Related
- 2006-11-17 AU AU2006314310A patent/AU2006314310B2/en not_active Ceased
- 2006-11-17 WO PCT/GB2006/004294 patent/WO2007057682A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
CN101351553B (zh) | 2012-09-05 |
CA2630183A1 (en) | 2007-05-24 |
JP5349051B2 (ja) | 2013-11-20 |
CA2630183C (en) | 2014-07-15 |
CN101351553A (zh) | 2009-01-21 |
AU2006314310B2 (en) | 2012-04-26 |
GB0523582D0 (en) | 2005-12-28 |
GB2432366A (en) | 2007-05-23 |
AU2006314310A1 (en) | 2007-05-24 |
JP2009515546A (ja) | 2009-04-16 |
DK1948794T3 (da) | 2011-10-24 |
EP1948794A1 (de) | 2008-07-30 |
ES2395249T3 (es) | 2013-02-11 |
GB2432366B (en) | 2007-11-21 |
EP1948794B1 (de) | 2011-07-13 |
PT1948794E (pt) | 2011-09-12 |
US20090203572A1 (en) | 2009-08-13 |
WO2007057682A1 (en) | 2007-05-24 |
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