DE60110926D1 - Eine methode zur in vitro molekularen evolution der proteinfunktion - Google Patents

Eine methode zur in vitro molekularen evolution der proteinfunktion

Info

Publication number
DE60110926D1
DE60110926D1 DE60110926T DE60110926T DE60110926D1 DE 60110926 D1 DE60110926 D1 DE 60110926D1 DE 60110926 T DE60110926 T DE 60110926T DE 60110926 T DE60110926 T DE 60110926T DE 60110926 D1 DE60110926 D1 DE 60110926D1
Authority
DE
Germany
Prior art keywords
single stranded
fragments
sequences
protein motifs
polynucleotide sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60110926T
Other languages
English (en)
Other versions
DE60110926T3 (de
DE60110926T2 (de
Inventor
Roland Carlsson
Christina Furebring
Hager Ann-Christin Malmborg
Carl Borrebaeck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alligator Bioscience AB
Original Assignee
Alligator Bioscience AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26245418&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60110926(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US09/734,801 external-priority patent/US6958213B2/en
Priority claimed from GB0030252A external-priority patent/GB2370038B/en
Application filed by Alligator Bioscience AB filed Critical Alligator Bioscience AB
Publication of DE60110926D1 publication Critical patent/DE60110926D1/de
Application granted granted Critical
Publication of DE60110926T2 publication Critical patent/DE60110926T2/de
Publication of DE60110926T3 publication Critical patent/DE60110926T3/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Ecology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60110926T 2000-12-12 2001-12-12 Eine methode zur in vitro molekularen evolution der proteinfunktion Expired - Lifetime DE60110926T3 (de)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US09/734,801 US6958213B2 (en) 2000-12-12 2000-12-12 Method for in vitro molecular evolution of protein function
GB0030252A GB2370038B (en) 2000-12-12 2000-12-12 A method for in vitro molecular evolution of protein function
GB0030252 2000-12-12
US734801 2000-12-12
PCT/EP2001/014744 WO2002048351A2 (en) 2000-12-12 2001-12-12 A method for in vitro molecular evolution of protein function
EP01985402A EP1341909B2 (de) 2000-12-12 2001-12-12 Eine methode zur in vitro molekularen evolution der proteinfunktion

Publications (3)

Publication Number Publication Date
DE60110926D1 true DE60110926D1 (de) 2005-06-23
DE60110926T2 DE60110926T2 (de) 2006-03-23
DE60110926T3 DE60110926T3 (de) 2012-06-14

Family

ID=26245418

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60110926T Expired - Lifetime DE60110926T3 (de) 2000-12-12 2001-12-12 Eine methode zur in vitro molekularen evolution der proteinfunktion

Country Status (10)

Country Link
EP (1) EP1341909B2 (de)
JP (1) JP4430303B2 (de)
AT (1) ATE295882T1 (de)
AU (2) AU3457202A (de)
CA (1) CA2430582C (de)
DE (1) DE60110926T3 (de)
DK (1) DK200300821A (de)
ES (1) ES2244670T5 (de)
PT (1) PT1341909E (de)
WO (1) WO2002048351A2 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030036641A1 (en) * 2001-01-31 2003-02-20 Padgett Hal S. Methods for homology-driven reassembly of nucleic acid sequences
WO2003097834A2 (en) * 2002-05-17 2003-11-27 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
AU2003250811A1 (en) * 2002-08-19 2004-03-03 Danmarks Tekniske Universitet (Dtu) Methods and kit for genes shuffling using tagged primers
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
GB0607798D0 (en) 2006-04-20 2006-05-31 Alligator Bioscience Ab Novel polypeptides and use thereof
GB0708376D0 (en) 2007-05-01 2007-06-06 Alligator Bioscience Ab Novel polypeptides and uses thereof
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
GB0905790D0 (en) 2009-04-03 2009-05-20 Alligator Bioscience Ab Novel polypeptides and use thereof
GB0920258D0 (en) 2009-11-19 2010-01-06 Alligator Bioscience Ab New medical agents and use thereof
GB201115280D0 (en) 2011-09-05 2011-10-19 Alligator Bioscience Ab Antibodies, uses and methods
GB201311475D0 (en) 2013-06-27 2013-08-14 Alligator Bioscience Ab Polypeptides
GB201311487D0 (en) 2013-06-27 2013-08-14 Alligator Bioscience Ab Bispecific molecules
CN106573981A (zh) 2014-08-12 2017-04-19 鳄鱼生物科学公司 利用抗cd40抗体的组合疗法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5851804A (en) * 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
AU5030900A (en) * 1999-05-21 2000-12-12 Penn State Research Foundation, The Construction of incremental truncation libraries

Also Published As

Publication number Publication date
CA2430582A1 (en) 2002-06-20
AU2002234572B2 (en) 2006-07-06
JP2004515248A (ja) 2004-05-27
JP4430303B2 (ja) 2010-03-10
EP1341909B2 (de) 2012-04-11
WO2002048351A3 (en) 2003-06-05
WO2002048351A2 (en) 2002-06-20
PT1341909E (pt) 2005-10-31
EP1341909B1 (de) 2005-05-18
EP1341909B8 (de) 2006-05-03
ATE295882T1 (de) 2005-06-15
DE60110926T3 (de) 2012-06-14
AU3457202A (en) 2002-06-24
ES2244670T5 (es) 2012-08-02
DE60110926T2 (de) 2006-03-23
DK200300821A (da) 2003-08-12
EP1341909A2 (de) 2003-09-10
CA2430582C (en) 2012-12-04
ES2244670T3 (es) 2005-12-16

Similar Documents

Publication Publication Date Title
DE60110926D1 (de) Eine methode zur in vitro molekularen evolution der proteinfunktion
ATE356871T1 (de) Eine methode zur in vitro molekularen evolution der proteinfunktion
ATE448301T1 (de) Sammlung von proteinen mit sich wiederholenden sequenzen (repeat proteins), die repetitive sequenzmodule enthalten
ATE409235T1 (de) Verfahren zur herstellung von ascorbinsäurezwischenprodukten in wirtszellen
BR9407712A (pt) Métodos e composiçoes para sequenciamento eficaz de ácidos nuclêicos
ATE469911T1 (de) Isolierte nukleinsäuremoleküle die t-zellen induzierbare faktoren kodieren (tif), kodierte proteine und ihre anwendung
DE60009323D1 (de) Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen
NZ333128A (en) Engineered human DNase I with DNA hydrolytic activity at least 50 percent greater than native human DNase I
ATE258982T1 (de) Verfahren zur herstellung von hantelförmigen dna- molekülen als expressionskonstrukte
DE69403642D1 (de) 7-deazapurin modifizierte oligonukleotide
DE69943286D1 (de) Fluoreszierende proteine aus nicht-biolumineszenten arten der klasse anthozoa, gene die diese proteine kodieren und deren verwendungen
ATE405676T1 (de) Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination
ATE335086T1 (de) Künstliche chromosomen, die die genetische information für die bildung von rekombinantem virus enthalten
GB2432366B (en) A method for in vitro molecular evolution of protein function
HUP0303517A2 (hu) Hímfertilitást közvetítő nukleotidszekvenciák és alkalmazásuk
AU4744501A (en) Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
WO2003048309A3 (en) Thermus thermophilus nucleic acid polymerases
JP2004515248A5 (de)
FI961202A0 (fi) Grb3-3-geeni, sen variantteja ja niiden käyttöjä
ATE271609T1 (de) Mch3, eine apoptosis-protease, kodierende nukleinsäuren und methoden zur verwendung
WO1990009457A3 (en) Oligonucleotide primer pairs for sequence independent gene amplification and methods which employ them
ATE303444T1 (de) Nukleinsäuren kodierend für das tmk gen
CO4440441A1 (es) Enzima dextranasa, metodo para su produccion y adn que la codifica
BR9915537A (pt) Gene prv-1 e sua utilização
NZ508270A (en) Recombinant secoisolariciresinol dehydrogenase, and methods of use

Legal Events

Date Code Title Description
R102 Epo decision maintaining patent in amended form now final

Ref document number: 1341909

Country of ref document: EP