ATE409235T1 - Verfahren zur herstellung von ascorbinsäurezwischenprodukten in wirtszellen - Google Patents
Verfahren zur herstellung von ascorbinsäurezwischenprodukten in wirtszellenInfo
- Publication number
- ATE409235T1 ATE409235T1 AT02741663T AT02741663T ATE409235T1 AT E409235 T1 ATE409235 T1 AT E409235T1 AT 02741663 T AT02741663 T AT 02741663T AT 02741663 T AT02741663 T AT 02741663T AT E409235 T1 ATE409235 T1 AT E409235T1
- Authority
- AT
- Austria
- Prior art keywords
- host cells
- ascorbic acid
- phosphorylates
- carbon
- enzymatic activity
- Prior art date
Links
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 title abstract 6
- 229960005070 ascorbic acid Drugs 0.000 title abstract 3
- 235000010323 ascorbic acid Nutrition 0.000 title abstract 3
- 239000011668 ascorbic acid Substances 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 239000013067 intermediate product Substances 0.000 title 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 4
- 229910052799 carbon Inorganic materials 0.000 abstract 4
- 230000002255 enzymatic effect Effects 0.000 abstract 4
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 abstract 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 2
- 239000000543 intermediate Substances 0.000 abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 230000006652 catabolic pathway Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 230000001590 oxidative effect Effects 0.000 abstract 1
- 230000037361 pathway Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/58—Aldonic, ketoaldonic or saccharic acids
- C12P7/60—2-Ketogulonic acid
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28157101P | 2001-04-04 | 2001-04-04 | |
| US28227701P | 2001-04-05 | 2001-04-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE409235T1 true ATE409235T1 (de) | 2008-10-15 |
Family
ID=26960952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02741663T ATE409235T1 (de) | 2001-04-04 | 2002-04-04 | Verfahren zur herstellung von ascorbinsäurezwischenprodukten in wirtszellen |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US7033804B2 (de) |
| EP (2) | EP2039774A1 (de) |
| JP (1) | JP2005510999A (de) |
| KR (2) | KR100932882B1 (de) |
| CN (1) | CN1298855C (de) |
| AT (1) | ATE409235T1 (de) |
| AU (1) | AU2002314738A1 (de) |
| CA (1) | CA2442552C (de) |
| DE (1) | DE60229050D1 (de) |
| DK (1) | DK1383903T3 (de) |
| WO (1) | WO2002081440A2 (de) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK1373480T3 (da) * | 2001-04-04 | 2009-05-11 | Genencor Int | Ukoblede produktive og katabolske værtscellebaner |
| DK1383903T3 (da) * | 2001-04-04 | 2009-03-23 | Genencor Int | Fremgangsmåder til fremstilling af askorbinsyre-intermediater i vært-celler |
| AU2003290036A1 (en) * | 2002-12-19 | 2004-07-14 | Basf Aktiengesellschaft | Method for extracting 2-ketone-l-gulonic acid from a polar, preferably aqueous solvent |
| WO2005017099A2 (en) | 2003-05-22 | 2005-02-24 | Genencor International, Inc. | Metabolically engineered bacterial strains having non-functional endogenous gluconate transporters |
| WO2005012486A2 (en) | 2003-07-30 | 2005-02-10 | Genencor International, Inc. | Metabolically engineered bacterial strains having enhanced 2-keto-d-gluconate accumulation |
| US9637746B2 (en) * | 2008-12-15 | 2017-05-02 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways |
| CN102666862A (zh) | 2009-08-21 | 2012-09-12 | 马斯科马公司 | 在联合生物加工生物体中生产丙醇、醇和多元醇 |
| JP2013526277A (ja) | 2010-05-07 | 2013-06-24 | グリーンライト バイオサイエンシーズ インコーポレーテッド | 酵素リロケーションにより代謝経路のフラックスを制御する方法 |
| KR101269810B1 (ko) * | 2010-07-15 | 2013-05-30 | 씨제이제일제당 (주) | Ｌ－라이신 생산능이 향상된 미생물 및 이를 이용한 ｌ－라이신 생산방법 |
| WO2012008810A2 (en) * | 2010-07-15 | 2012-01-19 | Cj Cheiljedang Corporation | Microorganism with enhanced l-lysine productivity and method for producing l-lysine using the same |
| KR101988063B1 (ko) | 2010-08-31 | 2019-09-30 | 그린라이트 바이오사이언시스, 아이엔씨. | 프로테아제 조작을 통한 대사 경로에서 유동의 제어 방법 |
| ES2387661B1 (es) * | 2010-12-10 | 2013-10-31 | Biolan Microbiosensores S.L. | Proceso de purificación y estabilización de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); y uso de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3). |
| EP2753702B1 (de) | 2011-09-09 | 2021-12-15 | GreenLight Biosciences, Inc. | Zellfreie herstellung von carbapenemen |
| CN102363759B (zh) * | 2011-10-27 | 2012-10-17 | 福州大学 | 一株产庆大霉素C1a的工程菌及其应用 |
| WO2014100722A1 (en) | 2012-12-21 | 2014-06-26 | Greenlight Biosciences, Inc. | Cell-free system for converting methane into fuel, pyruvate or isobutanol |
| US9688977B2 (en) | 2013-08-05 | 2017-06-27 | Greenlight Biosciences, Inc. | Engineered phosphoglucose isomerase proteins with a protease cleavage site |
| RU2017137788A (ru) | 2015-03-30 | 2019-04-30 | Гринлайт Байосайенсис, Инк. | Бесклеточное получение рибонуклеиновой кислоты |
| SG11201808721YA (en) | 2016-04-06 | 2018-11-29 | Greenlight Biosciences Inc | Cell-free production of ribonucleic acid |
| CN105695520B (zh) * | 2016-04-21 | 2019-12-24 | 江南大学 | 一种提高发酵法生产2-酮基-d-葡萄糖酸产量的方法 |
| WO2018129275A1 (en) | 2017-01-06 | 2018-07-12 | Greenlight Biosciences, Inc. | Cell-free production of sugars |
| JP7792196B2 (ja) | 2017-10-11 | 2025-12-25 | グリーンライト バイオサイエンシーズ インコーポレーテッド | ヌクレオシド三リン酸およびリボ核酸の生成のための方法および組成物 |
| CN112430560B (zh) * | 2019-08-26 | 2024-01-05 | 中国科学院分子植物科学卓越创新中心 | 一种2-酮基-l-古龙酸生产菌株及其构建方法 |
| CN111073946A (zh) * | 2019-12-23 | 2020-04-28 | 中国科学院沈阳应用生态研究所 | Vc二步发酵的营养优化方法 |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| US4757012A (en) | 1983-06-28 | 1988-07-12 | Genentech, Inc. | Ascorbic acid intermediates and process enzymes |
| US5004690A (en) | 1983-06-28 | 1991-04-02 | Genetech, Inc. | Ascorbic acid intermediates and process enzymes |
| US4758514A (en) | 1983-06-28 | 1988-07-19 | Genentech, Inc. | Ascorbic acid intermediates and process enzymes |
| DE3569537D1 (en) * | 1984-01-23 | 1989-05-24 | Akzo Nv | Process for the preparation of 2-keto-aldonic acids |
| US5008193A (en) | 1984-06-14 | 1991-04-16 | Genentech, Inc. | Ascorbic acid intermediates and process enzymes |
| DE3621781A1 (de) | 1985-07-05 | 1987-01-15 | Takeda Chemical Industries Ltd | Verfahren zur reinigung von l-ascorbinsaeure |
| US5032514A (en) | 1988-08-08 | 1991-07-16 | Genentech, Inc. | Metabolic pathway engineering to increase production of ascorbic acid intermediates |
| GB8826429D0 (en) | 1988-11-11 | 1988-12-14 | Univ Leeds Ind Service Ltd | Enzyme stabilisation systems |
| JP2779073B2 (ja) | 1991-02-22 | 1998-07-23 | キッコーマン株式会社 | 選択発酵法 |
| WO1992018637A1 (en) | 1991-04-16 | 1992-10-29 | F.Hoffmann-La Roche Ag | Method for the production of d-gluconic acid |
| US5272073A (en) | 1992-06-30 | 1993-12-21 | Purdue Research Foundation | Biocatalytic synthesis of catechol from glucose |
| US5376544A (en) | 1992-09-08 | 1994-12-27 | Rutgers The State University Of New Jersey | Enzymes for the production of 2-keto-L-gulonic acid |
| CN1048282C (zh) | 1993-07-09 | 2000-01-12 | 武田药品工业株式会社 | 生产2－酮－l－古洛糖酸的方法 |
| US5672509A (en) | 1993-08-25 | 1997-09-30 | Pfizer Inc. | hPDE IV-C: a human phosphodiesterase IV isozyme |
| US5985617A (en) | 1997-02-18 | 1999-11-16 | Liao; James C. | Microorganisms and methods for overproduction of DAHP by cloned PPS gene |
| US5686276A (en) | 1995-05-12 | 1997-11-11 | E. I. Du Pont De Nemours And Company | Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism |
| US5795761A (en) | 1996-01-11 | 1998-08-18 | Rutgers, The State University Of New Jersey | Mutants of 2,5-diketo-D-gluconic acid (2,5-DKG) reductase A |
| US5939307A (en) | 1996-07-30 | 1999-08-17 | The Archer-Daniels-Midland Company | Strains of Escherichia coli, methods of preparing the same and use thereof in fermentation processes for l-threonine production |
| CA2625060A1 (en) | 1996-11-13 | 1998-05-22 | E.I. Du Pont De Nemours And Company | Method for the production of glycerol by recombinant organisms |
| US6916646B1 (en) | 1997-06-23 | 2005-07-12 | Genencor International, Inc. | Enterobacteriaceae fermentation strains |
| CA2311601C (en) | 1997-12-02 | 2008-07-08 | E.I. Du Pont De Nemours And Company | Method for the production of glycerol by recombinant organisms |
| AU746542B2 (en) * | 1998-03-18 | 2002-05-02 | Ajinomoto Co., Inc. | L-glutamic acid-producing bacterium and method for producing L-glutamic acid |
| DE19818541C2 (de) | 1998-04-24 | 2003-04-10 | Forschungszentrum Juelich Gmbh | Mikrobielle Herstellung von Substanzen aus dem aromatischen Stoffwechsel / III |
| DE19823834A1 (de) | 1998-05-28 | 1999-12-02 | Basf Ag | Genetisches Verfahren zur Herstellung von Riboflavin |
| US6599722B2 (en) * | 1998-12-22 | 2003-07-29 | Genencor International, Inc. | Method for producing ascorbic acid intermediates |
| ATE335823T1 (de) | 1999-08-18 | 2006-09-15 | Du Pont | Verfahren zur biologischen herstellung von 1,3- propandiol |
| US6586214B1 (en) | 1999-09-15 | 2003-07-01 | Degussa Ag | Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene) |
| DK1383903T3 (da) | 2001-04-04 | 2009-03-23 | Genencor Int | Fremgangsmåder til fremstilling af askorbinsyre-intermediater i vært-celler |
| DK1373480T3 (da) * | 2001-04-04 | 2009-05-11 | Genencor Int | Ukoblede produktive og katabolske værtscellebaner |
-
2002
- 2002-04-04 DK DK02741663T patent/DK1383903T3/da active
- 2002-04-04 CA CA2442552A patent/CA2442552C/en not_active Expired - Lifetime
- 2002-04-04 AT AT02741663T patent/ATE409235T1/de not_active IP Right Cessation
- 2002-04-04 DE DE60229050T patent/DE60229050D1/de not_active Expired - Lifetime
- 2002-04-04 JP JP2002579428A patent/JP2005510999A/ja active Pending
- 2002-04-04 EP EP08015715A patent/EP2039774A1/de not_active Withdrawn
- 2002-04-04 KR KR1020037012986A patent/KR100932882B1/ko not_active Expired - Fee Related
- 2002-04-04 WO PCT/US2002/010949 patent/WO2002081440A2/en not_active Ceased
- 2002-04-04 CN CNB028076966A patent/CN1298855C/zh not_active Expired - Lifetime
- 2002-04-04 AU AU2002314738A patent/AU2002314738A1/en not_active Abandoned
- 2002-04-04 EP EP02741663A patent/EP1383903B1/de not_active Expired - Lifetime
- 2002-04-04 KR KR1020087028023A patent/KR20080113283A/ko not_active Ceased
- 2002-04-05 US US10/116,821 patent/US7033804B2/en not_active Expired - Lifetime
-
2005
- 2005-11-17 US US11/282,000 patent/US20060068477A1/en not_active Abandoned
-
2008
- 2008-02-27 US US12/038,373 patent/US20080254523A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| KR20040014482A (ko) | 2004-02-14 |
| US20080254523A1 (en) | 2008-10-16 |
| KR20080113283A (ko) | 2008-12-29 |
| US7033804B2 (en) | 2006-04-25 |
| EP1383903B1 (de) | 2008-09-24 |
| EP1383903A2 (de) | 2004-01-28 |
| DE60229050D1 (de) | 2008-11-06 |
| JP2005510999A (ja) | 2005-04-28 |
| US20060068477A1 (en) | 2006-03-30 |
| DK1383903T3 (da) | 2009-03-23 |
| AU2002314738A1 (en) | 2002-10-21 |
| CN1298855C (zh) | 2007-02-07 |
| CA2442552C (en) | 2012-06-26 |
| CA2442552A1 (en) | 2002-10-17 |
| WO2002081440A3 (en) | 2003-11-13 |
| CN1500148A (zh) | 2004-05-26 |
| EP1383903A4 (de) | 2004-12-15 |
| EP2039774A1 (de) | 2009-03-25 |
| US20030040086A1 (en) | 2003-02-27 |
| WO2002081440A2 (en) | 2002-10-17 |
| KR100932882B1 (ko) | 2009-12-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |