ATE409235T1 - Verfahren zur herstellung von ascorbinsäurezwischenprodukten in wirtszellen - Google Patents
Verfahren zur herstellung von ascorbinsäurezwischenprodukten in wirtszellenInfo
- Publication number
- ATE409235T1 ATE409235T1 AT02741663T AT02741663T ATE409235T1 AT E409235 T1 ATE409235 T1 AT E409235T1 AT 02741663 T AT02741663 T AT 02741663T AT 02741663 T AT02741663 T AT 02741663T AT E409235 T1 ATE409235 T1 AT E409235T1
- Authority
- AT
- Austria
- Prior art keywords
- host cells
- ascorbic acid
- phosphorylates
- carbon
- enzymatic activity
- Prior art date
Links
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 title abstract 6
- 229960005070 ascorbic acid Drugs 0.000 title abstract 3
- 235000010323 ascorbic acid Nutrition 0.000 title abstract 3
- 239000011668 ascorbic acid Substances 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 239000013067 intermediate product Substances 0.000 title 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 4
- 229910052799 carbon Inorganic materials 0.000 abstract 4
- 230000002255 enzymatic effect Effects 0.000 abstract 4
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 abstract 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 2
- 239000000543 intermediate Substances 0.000 abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 230000006652 catabolic pathway Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 230000001590 oxidative effect Effects 0.000 abstract 1
- 230000037361 pathway Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/58—Aldonic, ketoaldonic or saccharic acids
- C12P7/60—2-Ketogulonic acid
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28157101P | 2001-04-04 | 2001-04-04 | |
| US28227701P | 2001-04-05 | 2001-04-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE409235T1 true ATE409235T1 (de) | 2008-10-15 |
Family
ID=26960952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02741663T ATE409235T1 (de) | 2001-04-04 | 2002-04-04 | Verfahren zur herstellung von ascorbinsäurezwischenprodukten in wirtszellen |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US7033804B2 (de) |
| EP (2) | EP2039774A1 (de) |
| JP (1) | JP2005510999A (de) |
| KR (2) | KR20080113283A (de) |
| CN (1) | CN1298855C (de) |
| AT (1) | ATE409235T1 (de) |
| AU (1) | AU2002314738A1 (de) |
| CA (1) | CA2442552C (de) |
| DE (1) | DE60229050D1 (de) |
| DK (1) | DK1383903T3 (de) |
| WO (1) | WO2002081440A2 (de) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1373480B1 (de) * | 2001-04-04 | 2009-01-21 | Genencor International, Inc. | Entkoppelte anabole und katabole stoffwechselwege in wirtszellen |
| KR20080113283A (ko) | 2001-04-04 | 2008-12-29 | 제넨코 인터내셔날 인코포레이티드 | 숙주 세포에서의 생산물의 생산 방법 |
| EP1575968A1 (de) * | 2002-12-19 | 2005-09-21 | Basf Aktiengesellschaft | Verfahren zum extrahieren von 2-keto-l-gulonsäure (kgs) aus einem polaren, vorzugsweise wässrigen lösungsmittel |
| WO2005017099A2 (en) | 2003-05-22 | 2005-02-24 | Genencor International, Inc. | Metabolically engineered bacterial strains having non-functional endogenous gluconate transporters |
| WO2005012486A2 (en) | 2003-07-30 | 2005-02-10 | Genencor International, Inc. | Metabolically engineered bacterial strains having enhanced 2-keto-d-gluconate accumulation |
| US9637746B2 (en) * | 2008-12-15 | 2017-05-02 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways |
| BR112012003883A8 (pt) * | 2009-08-21 | 2018-02-06 | Mascoma Corp | Microorganismos recombinantes, processo de conversão de biomassa lignocelulósica em 1,2-propanodiol ou isopropanol, via metabólica engenheirada, agrupamento genético, e método de identificação de diol desidratase independente da vitamina b12 que converte propanodiol em propanal |
| ES2718844T3 (es) | 2010-05-07 | 2019-07-04 | Greenlight Biosciences Inc | Métodos para controlar el flujo en rutas metabólicas mediante la reubicación de enzimas |
| WO2012008810A2 (en) * | 2010-07-15 | 2012-01-19 | Cj Cheiljedang Corporation | Microorganism with enhanced l-lysine productivity and method for producing l-lysine using the same |
| KR101269810B1 (ko) * | 2010-07-15 | 2013-05-30 | 씨제이제일제당 (주) | Ｌ－라이신 생산능이 향상된 미생물 및 이를 이용한 ｌ－라이신 생산방법 |
| US8916358B2 (en) | 2010-08-31 | 2014-12-23 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through protease manipulation |
| ES2387661B1 (es) * | 2010-12-10 | 2013-10-31 | Biolan Microbiosensores S.L. | Proceso de purificación y estabilización de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3); y uso de la enzima gluconato deshidrogenasa (gadh, ec 1.1.99.3). |
| CA2846893A1 (en) | 2011-09-09 | 2013-03-14 | Greenlight Biosciences, Inc. | Cell-free preparation of carbapenems |
| CN102363759B (zh) * | 2011-10-27 | 2012-10-17 | 福州大学 | 一株产庆大霉素C1a的工程菌及其应用 |
| RU2015129774A (ru) | 2012-12-21 | 2017-02-01 | Гринлайт Байосайенсиз, Инк. | Бесклеточная система для преобразования метана в топливо, пируват или изобутанол |
| JP6483687B2 (ja) | 2013-08-05 | 2019-03-13 | グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． | プロテアーゼ切断部位を有する操作されたタンパク質 |
| BR112017020690A2 (pt) | 2015-03-30 | 2018-06-26 | Greenlight Biosciences Inc | produção livre de células de ácido ribonucleico |
| CN109196109B (zh) | 2016-04-06 | 2024-06-25 | 绿光生物科技股份有限公司 | 无细胞生成核糖核酸 |
| CN105695520B (zh) * | 2016-04-21 | 2019-12-24 | 江南大学 | 一种提高发酵法生产2-酮基-d-葡萄糖酸产量的方法 |
| AU2018205503A1 (en) | 2017-01-06 | 2019-07-25 | Greenlight Biosciences, Inc. | Cell-free production of sugars |
| BR112020007068A2 (pt) | 2017-10-11 | 2020-10-06 | Greenlight Biosciences, Inc. | métodos e composições para produção de nucleosídeo trifosfato e ácido ribonucleico |
| CN112430560B (zh) * | 2019-08-26 | 2024-01-05 | 中国科学院分子植物科学卓越创新中心 | 一种2-酮基-l-古龙酸生产菌株及其构建方法 |
| CN111073946A (zh) * | 2019-12-23 | 2020-04-28 | 中国科学院沈阳应用生态研究所 | Vc二步发酵的营养优化方法 |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| US4757012A (en) | 1983-06-28 | 1988-07-12 | Genentech, Inc. | Ascorbic acid intermediates and process enzymes |
| US5004690A (en) | 1983-06-28 | 1991-04-02 | Genetech, Inc. | Ascorbic acid intermediates and process enzymes |
| US4758514A (en) | 1983-06-28 | 1988-07-19 | Genentech, Inc. | Ascorbic acid intermediates and process enzymes |
| DE3569537D1 (en) * | 1984-01-23 | 1989-05-24 | Akzo Nv | Process for the preparation of 2-keto-aldonic acids |
| US5008193A (en) | 1984-06-14 | 1991-04-16 | Genentech, Inc. | Ascorbic acid intermediates and process enzymes |
| DE3621781A1 (de) | 1985-07-05 | 1987-01-15 | Takeda Chemical Industries Ltd | Verfahren zur reinigung von l-ascorbinsaeure |
| US5032514A (en) * | 1988-08-08 | 1991-07-16 | Genentech, Inc. | Metabolic pathway engineering to increase production of ascorbic acid intermediates |
| GB8826429D0 (en) | 1988-11-11 | 1988-12-14 | Univ Leeds Ind Service Ltd | Enzyme stabilisation systems |
| JP2779073B2 (ja) | 1991-02-22 | 1998-07-23 | キッコーマン株式会社 | 選択発酵法 |
| WO1992018637A1 (en) | 1991-04-16 | 1992-10-29 | F.Hoffmann-La Roche Ag | Method for the production of d-gluconic acid |
| US5272073A (en) | 1992-06-30 | 1993-12-21 | Purdue Research Foundation | Biocatalytic synthesis of catechol from glucose |
| US5376544A (en) | 1992-09-08 | 1994-12-27 | Rutgers The State University Of New Jersey | Enzymes for the production of 2-keto-L-gulonic acid |
| CN1048282C (zh) | 1993-07-09 | 2000-01-12 | 武田药品工业株式会社 | 生产2－酮－l－古洛糖酸的方法 |
| US5672509A (en) | 1993-08-25 | 1997-09-30 | Pfizer Inc. | hPDE IV-C: a human phosphodiesterase IV isozyme |
| US5985617A (en) | 1997-02-18 | 1999-11-16 | Liao; James C. | Microorganisms and methods for overproduction of DAHP by cloned PPS gene |
| US5686276A (en) | 1995-05-12 | 1997-11-11 | E. I. Du Pont De Nemours And Company | Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism |
| US5795761A (en) | 1996-01-11 | 1998-08-18 | Rutgers, The State University Of New Jersey | Mutants of 2,5-diketo-D-gluconic acid (2,5-DKG) reductase A |
| US5939307A (en) | 1996-07-30 | 1999-08-17 | The Archer-Daniels-Midland Company | Strains of Escherichia coli, methods of preparing the same and use thereof in fermentation processes for l-threonine production |
| EP0948628B1 (de) | 1996-11-13 | 2010-06-23 | E.I. Du Pont De Nemours And Company | Herstellungsverfahren von glycerin durch rekombinante organismen |
| US6916646B1 (en) | 1997-06-23 | 2005-07-12 | Genencor International, Inc. | Enterobacteriaceae fermentation strains |
| AU1619199A (en) | 1997-12-02 | 1999-06-16 | E.I. Du Pont De Nemours And Company | Method for the production of glycerol by recombinant organisms |
| AU746542B2 (en) * | 1998-03-18 | 2002-05-02 | Ajinomoto Co., Inc. | L-glutamic acid-producing bacterium and method for producing L-glutamic acid |
| DE19818541C2 (de) | 1998-04-24 | 2003-04-10 | Forschungszentrum Juelich Gmbh | Mikrobielle Herstellung von Substanzen aus dem aromatischen Stoffwechsel / III |
| DE19823834A1 (de) | 1998-05-28 | 1999-12-02 | Basf Ag | Genetisches Verfahren zur Herstellung von Riboflavin |
| US6599722B2 (en) * | 1998-12-22 | 2003-07-29 | Genencor International, Inc. | Method for producing ascorbic acid intermediates |
| DK1204755T3 (da) | 1999-08-18 | 2006-12-11 | Du Pont | Proces til biologisk produktion af 1,3-propandiol |
| US6586214B1 (en) | 1999-09-15 | 2003-07-01 | Degussa Ag | Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene) |
| KR20080113283A (ko) * | 2001-04-04 | 2008-12-29 | 제넨코 인터내셔날 인코포레이티드 | 숙주 세포에서의 생산물의 생산 방법 |
| EP1373480B1 (de) * | 2001-04-04 | 2009-01-21 | Genencor International, Inc. | Entkoppelte anabole und katabole stoffwechselwege in wirtszellen |
-
2002
- 2002-04-04 KR KR1020087028023A patent/KR20080113283A/ko not_active Ceased
- 2002-04-04 EP EP08015715A patent/EP2039774A1/de not_active Withdrawn
- 2002-04-04 CA CA2442552A patent/CA2442552C/en not_active Expired - Lifetime
- 2002-04-04 AU AU2002314738A patent/AU2002314738A1/en not_active Abandoned
- 2002-04-04 KR KR1020037012986A patent/KR100932882B1/ko not_active Expired - Fee Related
- 2002-04-04 DK DK02741663T patent/DK1383903T3/da active
- 2002-04-04 DE DE60229050T patent/DE60229050D1/de not_active Expired - Lifetime
- 2002-04-04 EP EP02741663A patent/EP1383903B1/de not_active Expired - Lifetime
- 2002-04-04 CN CNB028076966A patent/CN1298855C/zh not_active Expired - Lifetime
- 2002-04-04 AT AT02741663T patent/ATE409235T1/de not_active IP Right Cessation
- 2002-04-04 JP JP2002579428A patent/JP2005510999A/ja active Pending
- 2002-04-04 WO PCT/US2002/010949 patent/WO2002081440A2/en not_active Ceased
- 2002-04-05 US US10/116,821 patent/US7033804B2/en not_active Expired - Lifetime
-
2005
- 2005-11-17 US US11/282,000 patent/US20060068477A1/en not_active Abandoned
-
2008
- 2008-02-27 US US12/038,373 patent/US20080254523A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1383903A2 (de) | 2004-01-28 |
| DK1383903T3 (da) | 2009-03-23 |
| KR20040014482A (ko) | 2004-02-14 |
| EP1383903B1 (de) | 2008-09-24 |
| JP2005510999A (ja) | 2005-04-28 |
| CA2442552C (en) | 2012-06-26 |
| CA2442552A1 (en) | 2002-10-17 |
| US20060068477A1 (en) | 2006-03-30 |
| EP1383903A4 (de) | 2004-12-15 |
| WO2002081440A3 (en) | 2003-11-13 |
| KR20080113283A (ko) | 2008-12-29 |
| DE60229050D1 (de) | 2008-11-06 |
| EP2039774A1 (de) | 2009-03-25 |
| US7033804B2 (en) | 2006-04-25 |
| WO2002081440A2 (en) | 2002-10-17 |
| KR100932882B1 (ko) | 2009-12-21 |
| AU2002314738A1 (en) | 2002-10-21 |
| US20030040086A1 (en) | 2003-02-27 |
| CN1298855C (zh) | 2007-02-07 |
| US20080254523A1 (en) | 2008-10-16 |
| CN1500148A (zh) | 2004-05-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |