AR247920A1 - Un metodo mejorado para la transformacion intermediada por agrobacterium, de celulas de soja cultivadas. - Google Patents

Un metodo mejorado para la transformacion intermediada por agrobacterium, de celulas de soja cultivadas.

Info

Publication number
AR247920A1
AR247920A1 AR93325538A AR32553893A AR247920A1 AR 247920 A1 AR247920 A1 AR 247920A1 AR 93325538 A AR93325538 A AR 93325538A AR 32553893 A AR32553893 A AR 32553893A AR 247920 A1 AR247920 A1 AR 247920A1
Authority
AR
Argentina
Prior art keywords
agrobacterium
improved method
mediated transformation
soybean cells
species
Prior art date
Application number
AR93325538A
Other languages
English (en)
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25443700&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR247920(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Application granted granted Critical
Publication of AR247920A1 publication Critical patent/AR247920A1/es

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205Agrobacterium mediated transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

UN METODO INDEPENDIENTE DE GENOTIPO PARA PRODUCIR UNA PLANTA DE SOJA TRANSGENICA QUE COMPRENDE: A) COCULTIVAR UN EXPLANTO DERIVADO DE UN HIPOCOTILO O NODULOS COTILEDONARIOS CULTIVADOS DE UNA SEMILLA DE SOJA GERMINADA CON UNA ESPECIE DE AGROBACTERIUM QUE CONTIENE UN GEN QUIMERICO EN PRESENCIA DE UN COMPUESTO SENALADOR COMO ACETOSIRINGONA, ALFA- HIDROXIACETOSIRINGONA, ACETOVANILLONA, SIRINGALDEHIDO, ACIDO SIRINGICO Y ACIDO SINAPINICO Y MEZCLAS DE LOS MISMOS; B) MANTENER UNA TEMPERATURA PARA EL COCULTIVO DE DESDE 18 A 28*C; Y C) INDUCIR VIRULENCIA DEL AGROBACTERIUM DISMINUYENDO PH DEL MEDIO DE CULTIVO DE LA PLANTA POR DEBAJO DE PH 6, 0.
AR93325538A 1992-07-27 1993-07-27 Un metodo mejorado para la transformacion intermediada por agrobacterium, de celulas de soja cultivadas. AR247920A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US92040992A 1992-07-27 1992-07-27

Publications (1)

Publication Number Publication Date
AR247920A1 true AR247920A1 (es) 1995-04-28

Family

ID=25443700

Family Applications (1)

Application Number Title Priority Date Filing Date
AR93325538A AR247920A1 (es) 1992-07-27 1993-07-27 Un metodo mejorado para la transformacion intermediada por agrobacterium, de celulas de soja cultivadas.

Country Status (9)

Country Link
US (1) US5563055A (es)
EP (1) EP0652965A1 (es)
JP (2) JP2952041B2 (es)
AR (1) AR247920A1 (es)
AU (2) AU670316B2 (es)
BR (1) BR9306802A (es)
CA (1) CA2140910C (es)
HU (1) HUT70467A (es)
WO (1) WO1994002620A2 (es)

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