BR112023022181A2 - Métodos de geração de hepatócitos maduros - Google Patents

Métodos de geração de hepatócitos maduros

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Publication number
BR112023022181A2
BR112023022181A2 BR112023022181A BR112023022181A BR112023022181A2 BR 112023022181 A2 BR112023022181 A2 BR 112023022181A2 BR 112023022181 A BR112023022181 A BR 112023022181A BR 112023022181 A BR112023022181 A BR 112023022181A BR 112023022181 A2 BR112023022181 A2 BR 112023022181A2
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Brazil
Prior art keywords
methods
mature hepatocytes
generating mature
hepatocytes
generating
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BR112023022181A
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English (en)
Inventor
Ana D'alessio
Erin Kimbrel
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Astellas Inst For Regenerative Medicine
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Application filed by Astellas Inst For Regenerative Medicine filed Critical Astellas Inst For Regenerative Medicine
Publication of BR112023022181A2 publication Critical patent/BR112023022181A2/pt

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/70—Enzymes
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    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
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Abstract

métodos de geração de hepatócitos maduros. a presente invenção refere-se a métodos de geração de hepatócitos maduros aumentando a expressão de pelo menos um fator de transcrição selecionado do grupo que consiste em fator nuclear i x (nfix) e fator nuclear i c (nfic) em hepatócitos imaturos, e composições dos mesmos.
BR112023022181A 2021-05-07 2022-05-05 Métodos de geração de hepatócitos maduros BR112023022181A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163185735P 2021-05-07 2021-05-07
PCT/US2022/027776 WO2022235869A1 (en) 2021-05-07 2022-05-05 Methods of generating mature hepatocytes

Publications (1)

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BR112023022181A2 true BR112023022181A2 (pt) 2024-02-06

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BR112023022181A BR112023022181A2 (pt) 2021-05-07 2022-05-05 Métodos de geração de hepatócitos maduros

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US (1) US20240218332A1 (pt)
EP (1) EP4334435A1 (pt)
JP (1) JP2024518409A (pt)
KR (1) KR20240005887A (pt)
CN (1) CN117716020A (pt)
AU (1) AU2022270117A1 (pt)
BR (1) BR112023022181A2 (pt)
CA (1) CA3217861A1 (pt)
MX (1) MX2023013186A (pt)
TW (1) TW202309268A (pt)
WO (1) WO2022235869A1 (pt)

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EP4334435A1 (en) 2024-03-13
CN117716020A (zh) 2024-03-15
US20240218332A1 (en) 2024-07-04
CA3217861A1 (en) 2022-11-10
TW202309268A (zh) 2023-03-01
KR20240005887A (ko) 2024-01-12
JP2024518409A (ja) 2024-05-01
WO2022235869A1 (en) 2022-11-10
AU2022270117A1 (en) 2023-11-02
MX2023013186A (es) 2023-12-06

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