CN111164206A - 使用铁氧还蛋白-硫氧还蛋白还原酶增加植物生长和产量 - Google Patents

使用铁氧还蛋白-硫氧还蛋白还原酶增加植物生长和产量 Download PDF

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CN111164206A
CN111164206A CN201880059482.4A CN201880059482A CN111164206A CN 111164206 A CN111164206 A CN 111164206A CN 201880059482 A CN201880059482 A CN 201880059482A CN 111164206 A CN111164206 A CN 111164206A
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M·贝其曼
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Benson Hill Inc
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Abstract

本文提供了用于改善植物生长的组合物和方法。也提供了用于表达感兴趣基因的表达构建体、包括铁氧还蛋白‑硫氧还蛋白还原酶蛋白的多肽和编码铁氧还蛋白‑硫氧还蛋白还原酶蛋白的多核苷酸，包含该表达构建体、多核苷酸和多肽的植物，和产生转基因植物的方法，该感兴趣基因的表达可改善农业性质，包括但不限于作物产量、生物和非生物胁迫耐受性、和早期活力。

Description

使用铁氧还蛋白-硫氧还蛋白还原酶增加植物生长和产量
发明领域
本发明涉及通过在植物中表达铁氧还蛋白-硫氧还蛋白还原酶基因来增加植物生长和产量的组合物和方法。
背景技术
持续增加的世界人口和日渐减少的农业可耕种土地供应推动开发具有增加的生物质和产量的植物。常规农作物和园艺改进手段采用选择性育种技术来鉴定具有所需特性的植物。然而，这种选择性育种技术具有几个缺陷，即，这些技术一般是费力的并且产生通常含有异源遗传组件的植物，这可能并不总是导致所需的性状从亲本植物传递下去。分子生物学的进展提供了精确改良植物种质的手段。植物遗传改造要求分离和操作遗传物质(一般是DNA或RNA的形式)并且随后在植物中导入该遗传物质。这种技术具有向作物或植物递送各种改善的经济、农业或园艺性状的能力。
感兴趣的性状包括植物生物质和产量。产量通常定义为可测量的来自作物的有经济价值的产物。这可以量和/或质来定义。产量直接取决于几个因素，例如，器官的数量和尺寸、植物结构(例如，分枝数量)、种子产生、叶衰老等。根发育、营养摄入、胁迫耐受性、光合作用碳同化作用速率和早期活力也可能是决定产量的重要因素。因此，优化上述因素可有助于增加作物产量。
种子产量的增加是特别重要的性状，因为许多植物的种子对于人和动物消耗而言是重要的。作物例如玉米、水稻、小麦、油菜和大豆占人类总热量摄入的超过一半，无论是通过直接消耗它们的种子还是通过消耗在经加工的种子上产生的肉制品。它们也是工业过程中使用的糖、油和许多种代谢物的来源。种子含有胚(新芽和根的来源)和胚乳(在萌发期间和幼苗的早期生长期间供胚生长的营养源)。种子的发育涉及许多基因，并且需要将代谢物从根、叶和茎转移到生长的种子中。尤其是胚乳，其吸收了糖、油和蛋白质的代谢前体并将它们合成为储存大分子以填充谷粒。植物生物质的增加对于饲料作物如苜蓿、青贮玉米和干草而言重要。许多基因参与有助于植物生长和发育的代谢途径。调节植物中一种或多种此类基因的表达可产生相对于对照植物具有改善的生长和发育的植物，但通常可产生相对于对照植物具有受损的生长和发育的植物。因此，需要改善植物生长和发育的方法。
发明概述
提供用于调节植物中基因表达的组合物和方法。所述方法增加植物生长，导致较高作物产量。此类方法包括增加感兴趣植物中至少一种铁氧还蛋白-硫氧还蛋白还原酶基因的表达。本发明还包括含有启动子的构建体，所述启动子驱动植物细胞中与铁氧还蛋白-硫氧还蛋白还原酶编码序列操作性连接的表达。组合物还包含具有增加的铁氧还蛋白-硫氧还蛋白还原酶序列表达的植物，植物种子，植物器官，植物细胞和其他植物部分。本发明包括可以用于增加植物中铁氧还蛋白-硫氧还蛋白还原酶基因表达的方法。所述铁氧还蛋白-硫氧还蛋白还原酶基因可以是天然序列，或者可以是对感兴趣植物异源的序列。
本发明的实施方式包括：
1.一种增加作物产量的方法，所述方法包括用至少一种铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列转化植物。
2.如实施方式1所述的方法，其中所述铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列包含SEQ ID NO：1，或编码选自SEQ ID NO：2和14-106的蛋白质。
3.如实施方式1所述的方法，其中所述铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列编码与选自SEQ ID NO：2和14-106的序列具有至少80％，90％，95％，96％，97％，98％或99％序列相同性并且具有铁氧还蛋白-硫氧还蛋白还原酶功能的蛋白质。
4.如实施方式1所述的方法，其中所述铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列编码相对于选自SEQ ID NO：2和14-106的序列具有至少80％，85％，90％，95％，96％，97％，98％或99％序列阳性并且具有铁氧还蛋白-硫氧还蛋白还原酶功能的蛋白质。
5.一种植物，该植物基因组中稳定地纳入在植物中驱动表达的启动子，该启动子与铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列操作性地连接，其中所述启动子相对于所述铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列是异源的。
6.如实施方式5所述的植物，其中所述铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列包含SEQ ID NO：1，或编码选自SEQ ID NO：2和14-106的蛋白质。
7.如实施方式5所述的植物，其中所述铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列编码相对于选自SEQ ID NO：2和14-106的序列具有至少80％，85％，90％，95％，96％，97％，98％或99％序列相同性并且具有铁氧还蛋白-硫氧还蛋白还原酶功能的蛋白质。
8.如实施方式5所述的植物，其中所述铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列编码相对于选自SEQ ID NO：2和14-106的序列具有至少80％，85％，90％，95％，96％，97％，98％或99％序列阳性并且具有铁氧还蛋白-硫氧还蛋白还原酶功能的蛋白质。
9.实施方式5-8中任一项所述植物的经转化的种子。
10.如实施方式5-8中任一项所述的植物，其中所述植物是单子叶植物。
11.如实施方式10所述的植物，其中所述植物来自玉米属(Zea)，稻属(Oryza)，小麦属(Triticum)，高粱属(Sorghum)，黑麦属(Secale)，椮属(Eleusine)，粟属(Setaria)，甘蔗属(Saccharum)，芒属(Miscanthus)，黍属(Panicum)，狼尾草属(Pennisetum)，大锥属(Megathyrsus)，椰子属(Cocos)，凤梨属(Ananas)，芭蕉属(Musa)，油棕属(Elaeis)，燕麦属(Avena)或大麦属(Hordeum)。
12.如实施方式5-8中任一项所述的植物，其中所述植物是双子叶植物。
13.如实施方式12所述的植物，其中所述植物来自大豆属(Glycine)，芸苔属(Brassica)，苜蓿属(Medicago)，向日葵属(Helianthus)，红花属(Carthamus)，烟草属(Nicotiana)，茄属(Solanum)，棉属(Gossypium)，番薯属(Ipomoea)，木薯属(Manihot)，咖啡属(Coffea)，柑橘属(Citrus)，可可属(Theobroma)，山茶属(Camellia)，鳄梨属(Persea)，榕属(Ficus)，番石榴属(Psidium)，芒果属(Mangifera)，油橄榄属(Olea)，番木瓜属(Carica)，腰果属(Anacardium)，澳洲坚果属(Macadamia)，李属(Prunus)，甜菜属(Beta)，杨属(Populus)或桉属(Eucalyptus)。
14.如实施方式5-8中任一项所述的植物，其中所述植物相对于对照植物表现出增加的生长。
15.如实施方式5-8中任一项所述的植物，其中所述植物相对于对照植物表现出增加的生物量产量。
16.如实施方式5-8中任一项所述的植物，其中所述植物相对于对照植物表现出增加的种子产量。
17.如实施方式1-4中任一项所述的方法，其中所述铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列由发育调节型(developmentally regulated)启动子表达。
18.如实施方式17所述的方法，其中所述发育调节型启动子包含SEQ ID NO:5。
19.如实施方式1-18中任一项所述的方法，所述方法进一步包括用至少一种其它蛋白质编码序列转化植物。
20.如实施方式19所述的方法，其中所述至少一种其它蛋白质编码序列选自SEQID NO：8和11，或者编码与选自SEQ ID NO：9和12的序列具有至少90％相同性的蛋白质。
21.如实施方式19或20所述的方法，其中所述至少一种其它蛋白质编码序列编码选自SEQ ID NO：9和12的蛋白质。
22.如实施方式5-8中任一项所述的植物，其中在植物中驱动表达的所述启动子是发育调节型启动子。
23.如实施方式22所述的植物，其中所述发育启动子包含SEQ ID NO:5。
24.如实施方式5所述的植物，在其基因组中稳定地纳入在植物中驱动表达的第二启动子，该启动子与第二蛋白编码序列操作性地连接，其中所述第二启动子相对于所述第二蛋白编码序列是异源的。
25.如实施方式24所述的植物，其中所述第二蛋白质编码序列选自SEQ ID NO：8和11，或者编码与选自SEQ ID NO：9和12的序列具有至少90％相同性的蛋白质。
26.如实施方式24或25所述的植物，其中所述第二蛋白编码序列编码选自SEQ IDNO：9和12的蛋白质。
27.一种DNA构建体，所述DNA构建体包含操作性连接的，
a.在植物细胞中有功能的启动子，和
b.编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的核酸序列。
28.如实施方式27所述的DNA构建体，其中编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的所述核酸序列包含SEQ ID NO：1，或编码选自SEQ ID NO：2和14-106的蛋白质。
29.如实施方式27或28所述的DNA构建体，其中所述编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的核酸序列编码相对于选自SEQ ID NO：2和14-106的序列具有至少80％，85％，90％，95％，96％，97％，98％或99％序列相同性并且具有铁氧还蛋白-硫氧还蛋白还原酶功能的蛋白质。
30.如实施方式27或28所述的DNA构建体，其中所述编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的核酸序列编码相对于选自SEQ ID NO：2和14-106的序列具有至少80％，85％，90％，95％，96％，97％，98％或99％序列阳性并且具有铁氧还蛋白-硫氧还蛋白还原酶功能的蛋白质。
31.如实施方式27或28所述的DNA构建体，其中在植物细胞中有功能的所述启动子包含SEQ ID NO：5。
32.如实施方式27-31中任一项所述的DNA构建体，其中所述启动子相对于所述编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的核酸序列是异源的。
33.一种增加作物产量的方法，包括调节植物中至少一种铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列的表达。
34.如实施方式33所述的方法，其中所述调节表达包括增加植物中至少一种铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列的表达。
35.如实施方式34所述的方法，其中所述增加表达包括增加所述植物中天然铁氧还蛋白-硫氧还蛋白还原酶序列的活性或增加所述植物中天然铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列的活性。
36.如实施方式5-8中任一项所述的植物，其中所述在植物中驱动表达的启动子在叶组织中有活性。
37.如实施方式27-32中任一项所述的DNA构建体，其中所述在植物细胞中有功能的启动子在叶组织中有活性。
具体实施方式
提供用于增加作物生物质和产量的组合物和方法。所述方法包括增加感兴趣植物中至少一种铁氧还蛋白-硫氧还蛋白还原酶基因的表达。作物产量是极其复杂的性状，其来自作物植物在其发育的所有阶段的生长以及植物资源向植物的可收获部分的分配。在包括但不限于玉米和大豆的一些作物中，主要可收获部分可包括种子，次要应用来自生物质的剩余物(例如，叶和茎)。在包括但不限于甘蔗和苜蓿的其他作物中，植物的主要可收获部分由植物的茎或整个地上部分组成。在包括但不限于马铃薯和胡萝卜其他作物中，植物的主要可收获部分位于地下。无论作物植物的收获部分如何，可收获的生物质的积累都是由植物生长和光合固定碳分配到植物的收获部分的结果。可以通过调节一种或多种植物基因的表达来操纵植物生长。该调节可以改变一种或多种代谢途径的功能，所述代谢途径有助于植物生长和可收获生物质的积累。
本发明的方法包括通过调节编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的一种或多种基因的表达来操纵植物生长以增加产量。在一个优选的实施方式中，铁氧还蛋白-硫氧还蛋白还原酶蛋白编码基因的表达相对于对照植物中的铁氧还蛋白-硫氧还蛋白还原酶表达水平上调，导致相对于对照植物具有增加的铁氧还蛋白-硫氧还蛋白还原酶表达的植物中可收获的生物质增加。用于增加植物中铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列的活性或表达的任何方法都包括在本发明中。
本发明的组合物包括构建体，其包含SEQ ID NO：1所示的编码序列或包含编码选自SEQ ID NO：2和14-106或其变体的蛋白质的编码序列，所述编码序列操作性地连接于植物细胞中有功能的启动子。“启动子”意指DNA的调节区，其能够驱动植物或植物细胞中序列的表达。已经认识到，鉴定了本文公开的铁氧还蛋白-硫氧还蛋白还原酶蛋白序列后，分离和鉴定其他铁氧还蛋白-硫氧还蛋白还原酶蛋白序列以及编码铁氧还蛋白-硫氧还蛋白还原酶蛋白序列的核苷酸序列(例如通过BLAST搜索，PCR测定等)在本领域的技术范围内。
当在DNA构建体中组装本发明的编码序列从而启动子操作性地连接感兴趣的编码序列时，本发明的编码序列使得在用该DNA构建体稳定转化的植物细胞中表达并积累铁氧还蛋白-硫氧还蛋白还原酶蛋白。“操作性地连接”是指2个或更多个元件之间的功能性连接。例如，本发明的启动子和感兴趣的异源性核苷酸之间的操作性连接是功能性连接，其允许表达该感兴趣的异源性核苷酸序列。操作性地连接的元件可以是连续的或非连续的。当用于两个蛋白质编码区域之间的接合时，述及操作性地连接意在表示这些编码区域处于同一阅读框中。盒还可含有待共转化到植物中的至少一种其它基因。或者，可在多个表达盒或DNA构建体上提供其它基因。表达盒还可含有可选择的标志物基因。
由此，编码本发明的铁氧还蛋白-硫氧还蛋白还原酶蛋白的核苷酸序列在表达盒或表达构建体中伴随感兴趣的启动子序列(通常是异源性启动子序列)一起提供，用于在感兴趣的植物中表达。"异源性启动子序列"意在表示与铁氧还蛋白-硫氧还蛋白还原酶蛋白编码核苷酸序列非天然地操作性连接的序列。虽然铁氧还蛋白-硫氧还蛋白还原酶蛋白编码核苷酸序列和启动子序列彼此是异源的，但铁氧还蛋白-硫氧还蛋白还原酶蛋白编码核苷酸序列或异源启动子序列对于植物宿主可以是同源的，或天然的，或异源的，或外源的。公认地，启动子还可驱动其同源的或天然的核苷酸序列的表达。在该情况中，转化的植物将具有表型变化。
本发明的多核苷酸和氨基酸序列的片段和变体也可以由可在植物细胞中操作的启动子表达。“片段”是指多核苷酸的部分或氨基酸序列的部分。“变体”是指基本相似的序列。对于多核苷酸，变体包括具有以下的多核苷酸：在5'和/或3'端处的缺失(即，截短)；在天然多核苷酸中一个或多个内部位点处一个或多个核苷酸的缺失和/或添加；和/或在天然多核苷酸中一个或多个位点处一个或多个核苷酸的取代。本文所用的“天然”多核苷酸或多肽分别包含天然产生的核苷酸序列或氨基酸序列。一般而言，本发明的特定多核苷酸的变体将与特定核苷酸有至少约75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更大的序列相同性，如由本文他处所述的序列比对程序和参数所确定。本文公开的多核苷酸的片段和变体可以编码保留铁氧还蛋白-硫氧还蛋白还原酶功能的蛋白质。
“变体”氨基酸或蛋白是指通过下述过程衍生自天然氨基酸或蛋白质的氨基酸或蛋白质：在天然蛋白质的N-末端和/或C-末端处缺失(也称为截短)一个或多个氨基酸，在天然蛋白质的一个或多个内部位点处缺失和/或添加一个或多个氨基酸，或在天然蛋白质的一个或多个位点处取代一个或多个氨基酸。本发明包括的变体蛋白质有生物活性，即它们继续具有天然蛋白质的所需生物活性，诸如将还原的铁氧还蛋白转化成还原的硫氧还蛋白以调节酶活性。天然多肽的生物活性变体将与由本文所述的序列比对程序和参数确定的原始序列的氨基酸序列具有至少约80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更大的序列相同性。在一些实施方式中，变体多肽序列将包含保守氨基酸取代。可以使用此类保守氨基酸取代的数目与氨基酸相同性数目的加和来计算序列阳性，用该加和除以感兴趣序列中的氨基酸总数。序列阳性计算在NCBI BLAST服务器上进行，该服务器可在万维网上以blast.ncbi.nlm.nih.gov/Blast.cgi访问。本发明的蛋白质的生物活性变体与该蛋白质可相差少至1-15个氨基酸残基，少至1-10个，如6-10个，少至5个，少至4、3、2或甚至1个氨基酸残基。
氨基酸通常可以分类为脂族的，含羟基或硫/硒的，环形的，芳族的，碱性的或酸性的及其酰胺。不受理论限制，保守氨基酸取代在某些情况下可能比非保守氨基酸取代优选用于产生变体蛋白质序列，因为保守取代可能比非保守取代更可能允许变体蛋白质保留其生物活性。编码序列中具有一个或多个氨基酸取代的多肽的多核苷酸涵盖在本发明的范围内。下表1提供属于各类别的氨基酸的示例。
表1：氨基酸类型
Figure BDA0002408987160000081
也可通过分析经测序的基因组的现有数据库来鉴定变体序列。在这种方式中，可鉴定相应序列并用于本发明的方法中。
比对序列用于比较的方法是本领域熟知的。因此，可采用数学算法确定任意两个序列的序列相同性百分数。该数学算法的非限制性示例是Myers和Miller(1988)CABIOS 4:11-17的算法；Smith等.(1981)Adv.Appl.Math.2:482的局部比对算法；Needleman和Wunsch(1970)J.Mol.Biol.48:443-453的全局比对算法；Pearson和Lipman(1988)Proc.Natl.Acad.Sci.85:2444-2448的搜索局部比对方法；Karlin和Altschul(1990)Proc.Natl.Acad.Sci.USA 87:2264-2268的算法，由Karlin和Altschul(1993)Proc.Natl.Acad.Sci.USA 90:5873-5877改良。
这些数学算法的计算机实施手段可用于比较序列来确定序列相同性。这类实施手段包括但不限于：PC/Gene程序中的CLUSTAL(购自美国加利福尼亚州芒廷维尤的智慧遗传公司(Intelligenetics,Mountain View,California)；ALIGN程序(2.0版)和GCGWisconsin遗传软件包中的GAP,BESTFIT、BLAST、FASTA和TFASTA，第10版(购自阿克赛勒里公司(Accelrys Inc.)，美国加利福尼亚州圣地亚哥Scranton路9685号)。可用默认参数来进行使用这些程序的比对。CLUSTAL程序由以下详细描述：Higgins等，(1988)Gene 73:237-244(1988)；Higgins等，(1989)CABIOS 5:151-153；Corpet等，(1988)Nucleic AcidsRes.16:10881-90；Huang等，(1992)CABIOS 8:155-65；和Pearson等，(1994)Meth.Mol.Biol.24:307-331。ALIGN程序基于Myers和Miller(1988)(同上)的算法。比较氨基酸序列时，PAM120权重残基表、缺口长度罚分12、和缺口罚分4可与ALIGN程序联用。Altschul等，(1990)J.Mol.Biol.215:403的BLAST程序基于Karlin和Altschul(1990)(同上)的算法。可利用BLASTN程序进行BLAST核苷酸搜索(评分＝100，字长＝12)，以获得与编码本发明蛋白质的核苷酸序列同源的核苷酸序列。可利用BLASTX程序进行BLAST蛋白质搜索(评分＝50，字长＝3)，以获得与本发明蛋白质或多肽同源的氨基酸序列。为了获得缺口比对(出于比较目的)，可如Altschul等，(1997)Nucleic Acids Res.，25:3389所述利用缺口BLAST(在BLAST 2.0中)。或者，可利用PSI-BLAST(在BLAST 2.0中)进行迭代搜索，其用来检测分子之间的远近关系。参见Altschul等，(1997)同上。利用BLAST、缺口BLAST和PSI-BLAST程序时，可使用各程序(例如针对蛋白质的BLASTX，针对核苷酸的BLASTN)的默认参数。参见See www.ncbi.nlm.nih.gov。也可通过检查来人工进行比对。
可以对这些基因和编码区进行密码子优化以在感兴趣的植物中表达。"密码子优化的基因"是这样的基因，其密码子使用频率经设计以模拟宿主细胞的偏好密码子使用频率。核酸分子可以是完全或部分优化的密码子。因为任一氨基酸(除了甲硫氨酸和色氨酸)均由多种密码子编码，所述核酸分子的序列可变化但不改变编码的氨基酸。密码子优化是在核酸水平上改变一种或多种密码子时，致使氨基酸不变，但在具体的宿主生物体中的表达增加。本领域普通技术人员将知晓密码子表格，并且，提供关于广泛生物体的偏好信息的其它参考文献是本领域中可得的(参见例如，Zhang等.(1991)Gene 105:61-72；Murray等.(1989)Nucl.Acids Res.17:477-508)。优化用于在植物中表达的核苷酸序列的方法学提供于，例如，美国专利号6,015,891和其中引用的参考文献，以及WO2012/142371和其中引用的参考文献。
本发明的核苷酸序列可用于重组多核苷酸。“重组多核苷酸”包含两个或更多个化学连接的核酸区段的组合，这些区段未被发现在自然中直接接合。“直接接合”指2个核酸区段紧邻并且通过化学键互相接合。在具体实施方式中，重组多核苷酸包含感兴趣多核苷酸或其活性变体或片段，使得其他化学连接的核酸区段位于感兴趣多核苷酸的5’、3’或内部。或者，可通过删除序列来形成重组多核苷酸的化学连接的核酸区段。其他化学连接的核酸区段或被删除以接合所述连接的核酸区段的序列可以是任意长度，包括，例如，1、2、3、4、5、6、7、8、9、10、15、20或更多核苷酸。本文公开了用于制备这种重组多核苷酸的各种方法，例如，通过化学合成或通过遗传工程技术对多核苷酸的分离区段进行操作。在具体实施方式中，重组多核苷酸可包含重组DNA序列或重组RNA序列。“重组多核苷酸的片段”包含两个或更多个化学连接的氨基酸区段的组合中的至少一个，这些区段未被发现在自然中直接接合。
“改变”或“调节”基因的表达水平是指上调或下调所述基因的表达。公认地，在一些情况中，通过增加编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的一种或多种基因的表达水平(即，上调表达)来增加植物生长和产量。类似地，在一些情况中，可通过降低编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的一种或多种基因的表达水平(即，下调表达)来增加植物生长和产量。因此，本发明包括编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的一种或多种基因的上调或下调。此外，所述方法包括在感兴趣的植物中的编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的至少一种基因的上调和编码第二铁氧还蛋白-硫氧还蛋白还原酶蛋白的至少一种基因的下调。调节所需转基因植物中编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的至少一种基因的浓度和/或活性，意图使所述浓度和/或活性相对于不具有导入本发明的序列的原始对照植物、植物部分或细胞来说增大或减小至少约1％、约5％、约10％、约20％、约30％、约40％、约50％、约60％、约70％、约80％，或约90％，或更多。
一般认为，编码本发明在转基因植物中蛋白的基因的表达水平可通过采用在植物细胞中有功能的一种或多种启动子来控制。感兴趣的编码在转基因植物中蛋白的基因的表达水平可直接检测，例如，通过测定植物中的铁氧还蛋白-硫氧还蛋白还原酶转录本的水平或编码的蛋白质的水平。所述测定的方法是本领域熟知的。例如，Northern印迹或定量逆转录酶-PCR(qRT-PCR)可用于评估转录本水平，而western印迹、ELISA测定或酶测定可用于评估蛋白质水平。例如，可以通过对重组类囊体中NADP-MDH的活化进行评估来评估铁氧还蛋白-硫氧还蛋白还原酶功能(Droux等(1987)Arch Biochem Biophys252:426-439)。
“对象植物或植物细胞”是对于感兴趣的铁氧还蛋白-硫氧还蛋白还原酶蛋白编码基因的遗传改变(如转化)已经实现的植物或植物细胞，或者是源自如此改变的植物或细胞并包含该改变的植物或植物细胞。“对照”或“对照植物”或“对照植物细胞”提供了测量对象植物或植物细胞的表型变化的参照点。因此，根据本发明的方法，感兴趣的铁氧还蛋白-硫氧还蛋白还原酶蛋白编码基因的表达水平高于或低于对照植物中的表达水平。
一种对照植物或植物细胞可包含，例如：(a)野生型植物或细胞，即具有与用于产生对象植物或细胞的遗传改变的起始材料相同的基因型；(b)与起始材料有相同基因型但已经用无效构建体(即，用对感兴趣性状没有已知影响的构建体，如包含标记基因的构建体)转化的植物或植物细胞；(c)植物或植物细胞，其是对象植物或植物细胞的后代中的非转化分离体；(d)与对象植物或植物细胞在遗传上相同但没有接触会诱导感兴趣基因表达的条件或刺激的植物或植物细胞；或(e)在不表达感兴趣基因的条件下的对象植物或植物细胞本身。
虽然本发明以转化的植物描述，应认识到本发明的转化的生物体可包括植物细胞、植物原生质体、可再生出植物的植物组织培养物、植物愈伤组织、植物块、和在植物或植物部分中完整的植物细胞如胚胎、花粉、胚珠、种子、叶、花、枝条、果实、仁、谷穗、穗轴、外壳、柄、根、根尖、花粉囊等。谷粒是指由商业种植者出于生长或繁殖物种以外的目的产生的成熟种子。再生植物的后代、变体和突变体也包括在本发明的范围内，只要这些部分包含导入的多核苷酸。
为了下调感兴趣的铁氧还蛋白-硫氧还蛋白还原酶蛋白编码基因的表达，可构建与感兴趣基因(特别是编码感兴趣的铁氧还蛋白-硫氧还蛋白还原酶蛋白的基因)序列的信使RNA(mRNA)的至少一部分互补的反义构建体。构建反义核苷酸以与相应mRNA杂交。可进行反义序列的修饰，只要该序列杂交并干扰相应mRNA的表达。由此，可使用与待沉默的相应序列有70％，较佳80％，更佳85％、90％、95％或更大的序列相同性的反义构建形式。此外，可使用反义核苷酸的部分来破坏靶基因的表达。
可使用本发明的多核苷酸来从其他植物中分离相应序列。由此，PCR、杂交等方法可用于根据此类序列与本文所示序列的序列同源性或相同性来鉴定该此类序列。根据该序列与本文所示完整序列的或与其变体和片段的序列相同性而分离的序列涵盖在本发明中。此类序列包括公开序列的直向同源物序列。“直向同源物”是指源自共同祖先基因且由于物种形成而在不同物种中发现的基因。当在不同物种中发现的基因的核苷酸序列和/或它们的编码蛋白质序列具有至少75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更大的序列相同性时，认为基因是直向同源物。直向同源物的功能通常在物种之间高度保守。因此，本发明涵盖分离的多核苷酸，该分离的多核苷酸具有转录活化或增强子活性，并且与本文公开的序列或与其变体或片段具有至少75％的序列相同性。
可通过PCR分离变体序列。设计PCR引物和PCR克隆的方法是本领域熟知的并且公开于Sambrook等，(1989)《分子克隆：实验室手册》(Molecular Cloning:A LaboratoryManual)(第2版，纽约州普莱恩维尤的冷泉港出版社)。还参见Innis等编，(1990)《PCR方案：方法和应用指南》(PCR Protocols:A Guide to Methods and Applications)(纽约学术出版社)；Innis和Gelfand编，(1995)《PCR策略》(PCR Strategies)(纽约学术出版社)；以及Innis和Gelfand编，(1999)《PCR方法手册》(PCR Methods Manual)(纽约学术出版社)。
也可通过分析经测序的基因组的现有数据库来鉴定变体序列。通过这种方式，可鉴定编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的相应序列并将其用于本发明的方法中。变体序列将保留铁氧还蛋白-硫氧还蛋白还原酶蛋白的生物活性(即，催化还原的铁氧还蛋白转化成还原的硫氧还蛋白以调节酶活性)。出人意料地，本发明显示了铁氧还蛋白-硫氧还蛋白还原酶蛋白过表达的某些新型表达策略可以导致生物质和种子产量的增加。
表达盒将以5'-3'的转录方向包括，转录和翻译起始区，编码本发明铁氧还蛋白-硫氧还蛋白还原酶蛋白的多核苷酸，和在植物中有功能的转录和翻译终止区(即，终止区)。
本发明的实践中可使用多种启动子。编码本发明铁氧还蛋白-硫氧还蛋白还原酶蛋白的多核苷酸可以由具有组成型表达谱的启动子表达。组成型启动子包括CaMV 35S启动子(Odell等，(1985)Nature 313:810-812)；水稻肌动蛋白(McElroy等，(1990)Plant Cell2:163-171)；泛素(Christensen等，(1989)Plant Mol.Biol.12:619-632和Christensen等，(1992)Plant Mol.Biol.18:675-689)；pEMU(Last等，(1991)Theor.Appl.Genet.81:581-588)；MAS(Velten等，(1984)EMBO J.3:2723-2730)；ALS启动子(美国专利号5,659,026)等。
编码本发明铁氧还蛋白-硫氧还蛋白还原酶蛋白的本发明多核苷酸可以由组织偏好启动子表达。组织偏好启动子包括Yamamoto等，(1997)Plant J.12(2):255-265；Kawamata等，(1997)Plant Cell Physiol.38(7):792-803；Hansen等，(1997)Mol.GenGenet.254(3):337-343；Russell等，(1997)Transgenic Res.6(2):157-168；Rinehart等，(1996)Plant Physiol.112(3):1331-1341；Van Camp等，(1996)Plant Physiol.112(2):525-535；Canevascini等，(1996)Plant Physiol.112(2):513-524；Yamamoto等，(1994)Plant Cell Physiol.35(5):773-778；Lam(1994)Results Probl.Cell Differ.20:181-196；Orozco等，(1993)Plant Mol Biol.23(6):1129-1138；Matsuoka等，(1993)ProcNatl.Acad.Sci.USA90(20):9586-9590；和Guevara-Garcia等，(1993)Plant J.4(3):495-505。叶偏好启动子也是本领域已知的。参见，例如，Yamamoto等，(1997)Plant J.12(2):255-265；Kwon等，(1994)Plant Physiol.105:357-67；Yamamoto等，(1994)Plant CellPhysiol.35(5):773-778；Gotor等，(1993)Plant J.3:509-18；Orozco等，(1993)PlantMol.Biol.23(6):1129-1138；和Matsuoka等，(1993)Proc.Natl.Acad.Sci.USA 90(20):9586-9590。
发育调节型启动子可能是表达编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的多核苷酸所需的。此类启动子可在特定发育阶段显示表达峰。此类启动子已在本领域中描述，例如US 62/029,068；Gan和Amasino(1995)Science 270:1986-1988；Rinehart等(1996)PlantPhysiol 112:1331-1341；Gray-Mitsumune等(1999)Plant Mol Biol 39:657-669；Beaudoin和Rothstein(1997)Plant Mol Biol 33:835-846；Genschik等(1994)Gene 148:195-202等。
施用特定生物和/或非生物胁迫后被诱导的启动子可能是表达编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的多核苷酸所需的。此类启动子已在本领域中描述，例如，Yi等(2010)Planta 232:743-754；Yamaguchi-Shinozaki和Shinozaki(1993)Mol Gen Genet236:331-340；美国专利号7,674,952；Rerksiri等(2013)Sci World J 2013:文章号397401；Khurana等(2013)PLoS One 8:e54418；Tao等(2015)Plant Mol Biol Rep 33:200-208等。
细胞偏好型启动子可能是表达编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的多核苷酸所需的。这些启动子可以优先驱动特定细胞类型(例如叶肉细胞或维管束鞘细胞)中下游基因的表达。这种细胞偏好启动子已在本领域中描述，例如Viret等(1994)Proc Natl AcadUSA 91:8577-8581；美国专利号8,455,718；美国专利号7,642,347；Sattarzadeh等(2010)Plant Biotechnol J 8:112-125；Engelmann等(2008)Plant Physiol 146:1773-1785；Matsuoka等(1994)Plant J6:311-319等。
认识到特异性非组成型表达概况可相对于一种或多种感兴趣基因的组成型表达提供改善的植物表型。例如，通过光条件、施加特定应力、昼夜循环、或植物发育阶段来调节许多植物基因。这些表达概况对于植物中基因或基因产物的功能而言是非常重要的。可用于提供所需的表达概况的一个策略是使用含有在植物中所需的时间和位置处驱动所需的表达水平的顺式调节元件的合成启动子。已经在科学文献中描述了可用于在植物中改变基因表达的顺式调节元件(Vandepoele等，(2009)Plant Physiol 150:535-546；Rushton等，(2002)Plant Cell 14:749-762)。顺式调节元件也可用于改变启动子表达概况，如Venter(2007)Trends Plant Sci 12:118-124所述。
本领域已知植物终止子并且包括来自根癌土壤杆菌(A.tumefaciens)的Ti-质粒的那些，如真蛸碱合酶和胆脂碱合酶终止区。还参见Guerineau等，(1991)Mol.Gen.Genet.262:141-144；Proudfoot(1991)Cell 64:671-674；Sanfacon等(1991)Genes Dev.5:141-149；Mogen等，(1990)Plant Cell 2:1261-1272；Munroe等，(1990)Gene91:151-158；Ballas等，(1989)Nucleic Acids Res.17:7891-7903；和Joshi等，(1987)Nucleic Acids Res.15:9627-9639。
如上所述，本发明的编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的核苷酸可用于表达盒中，以转化感兴趣的植物。转化方案以及向植物中导入多肽或多核苷酸序列的方案可根据转化靶向的植物或植物细胞的类型(即，单子叶或双子叶)而变化。术语“转化”或“转化法”是指用于将多肽或多核苷酸引入植物细胞的任何方法。向植物细胞中导入多肽和多核苷酸的合适方法包括微注射(Crossway等，(1986)Biotechniques 4:320-334)、电穿孔(Riggs等，(1986)Proc.Natl.Acad.Sci.USA 83:5602-5606)，农杆菌-介导的转化(美国专利号5,563,055和美国专利号5,981,840)，直接基因转化(Paszkowski等，(1984)EMBO J.3:2717-2722)，和弹道颗粒加速(参见例如，美国专利号4,945,050；美国专利号5,879,918；美国专利号5,886,244；和5,932,782；Tomes等，(1995)《植物细胞、组织和器官培养中的基础方法》(Plant Cell,Tissue,and Organ Culture:Fundamental Methods)，Gamborg和Phillips编(Springer-Verlag,Berlin)；McCabe等，(1988)Biotechnology 6:923-926)；和Lec1转化(WO 00/28058)。还参见Weissinger等，(1988)Ann.Rev.Genet.22:421-477；Sanford等，(1987)Particulate Science and Technology 5:27-37(洋葱)；Christou等，(1988)Plant Physiol.87:671-674(大豆)；McCabe等，(1988)Bio/Technology 6:923-926(大豆)；Finer和McMullen(1991)In Vitro Cell Dev.Biol.27P:175-182(大豆)；Singh等，(1998)Theor.Appl.Genet.96:319-324(大豆)；Datta等，(1990)Biotechnology8:736(水稻)；Klein等，(1988)Proc.Natl.Acad.Sci.USA 85:4305-4309(玉米)；Klein等，(1988)Biotechnology 6:559-563(玉米)；美国专利号5,240,855；5,322,783；和5,324,646；Klein等，(1988)Plant Physiol.91:440-444(玉米)；Fromm等，(1990)Biotechnology 8:833-839(玉米)；Hooykaas-Van Slogteren等，(1984)Nature(伦敦)311:763-764；美国专利号5,736,369(谷类)；Bytebier等，(1987)Proc.Natl.Acad.Sci.USA 84:5345-5349(百合科(Liliaceae))；De Wet等，(1985)《胚珠组织实验操作》(The Experimental Manipulationof Ovule Tissues)，Chapman等编，(纽约朗文出版社(Longman,New York)，第197-209页(花粉)；Kaeppler等，(1990)Plant Cell Reports 9:415-418和Kaeppler等，(1992)Theor.Appl.Genet.84:560-566(须-介导的转化)；D'Halluin等，(1992)Plant Cell 4:1495-1505(电穿孔)；Li等，(1993)Plant Cell Reports 12:250-255以及Christou和Ford(1995)Annals of Botany 75:407-413(水稻)；Osjoda等，(1996)Nature Biotechnology14:745-750(玉米，通过根癌农杆菌(Agrobacterium tumefaciens))；其全部通过引用纳入本文。“稳定转化”是指导入植物的核苷酸构建体整合到植物的基因组中并且能够被其后代遗传。
按照常规方式，已经转化的细胞可长成植物。参见，例如，McCormick等，(1986)Plant Cell Reports 5:81-84。由此，本发明提供了转化的种子(也称为“转基因种子”，所述种子具有稳定纳入其基因组中的本发明的多核苷酸，例如，本发明的表达盒。
本发明可用于任何植物物种(包括但不限于单子叶和双子叶)的转化。感兴趣植物物种的示例包括但不限于，玉米(Zea mays)，油菜种(例如，甘蓝型油菜(B.napus)、白菜型油菜(B.rapa)、芥菜型油菜(B.juncea))，尤其是用作菜籽油来源的那些油菜物种，苜蓿(Medicago sativa)，水稻(Oryza sativa)，黑麦(Secale cereale)，高粱(Sorghumbicolor,Sorghum vulgare)，粟(例如，珍珠粟(Pennisetum glaucum)，黍(Panicummiliaceum)，小米(Setaria italica)，穇子(Eleusine coracana))，向日葵(Helianthusannuus)，藜(Chenopodium quinoa)，菊苣(Cichorium intybus)，莴苣(Lactuca sativa),红花(Carthamus tinctorius)，小麦(Triticum aestivum)，大豆(Glycine max)，烟草(Nicotiana tabacum)，马铃薯(Solanum tuberosum)，花生(Arachis hypogaea)，棉花(Gossypium barbadense,Gossypium hirsutum)，甘薯(Ipomoea batatas)，木薯(Manihotesculenta)，咖啡(Coffea spp.)，椰子(Cocos nucifera)，菠萝(Ananas comosus)，柠檬树(Citrus spp.)，可可(Theobroma cacao)，茶(Camellia sinensis)，香蕉(Musa spp.)，鳄梨(Persea americana)，无花果(Ficus casica)，番石榴(Psidium guajava)，芒果(Mangifera indica)，橄榄(Olea europaea)，番木瓜(Carica papaya)，腰果(Anacardiumoccidentale)，澳洲坚果(Macadamia integrifolia)，杏(Prunus amygdalus)、甜菜(Betavulgaris)、甘蔗(Saccharum spp.)、油棕榈(Elaeis guineensis)、白杨(杨树属(Populusspp.))、桉树(Eucalyptus spp.)、燕麦(Avena sativa)、大麦(Hordeum vulgare)、蔬菜、观赏植物和针叶植物。
在一个实施方式中，使用构建体转化一个或多个植物细胞，所述构建体含有在植物细胞中可操作的启动子，该启动子与编码本发明铁氧还蛋白-硫氧还蛋白还原酶蛋白的编码序列操作性连接。将一个或多个转化的植物细胞再生以产生转化的植物。用包含驱动本发明铁氧还蛋白-硫氧还蛋白还原酶蛋白编码多核苷酸表达的功能性启动子的构建体转化的这些植物表现出增加的植物产量，即增加的地上生物质和/或增加的可收获生物质和/或增加的种子产量。
目前，已证明上调铁氧还蛋白-硫氧还蛋白还原酶能增加植物产量，可采用用于增加感兴趣的植物中的内源性铁氧还蛋白-硫氧还蛋白还原酶序列的表达的其它方法。植物的基因组中存在的铁氧还蛋白-硫氧还蛋白还原酶基因的表达可通过在所述植物的基因组中存在的铁氧还蛋白-硫氧还蛋白还原酶基因的上游插入转录增强子来改变。该策略将允许铁氧还蛋白-硫氧还蛋白还原酶基因的表达，以维持其正常发育概况，同时显示升高的转录本水平。该策略将采用针对感兴趣的基因组序列设计的大范围核酸酶，通过在感兴趣的铁氧还蛋白-硫氧还蛋白还原酶基因上游插入增强子元件来实现。或者，采用与针对感兴趣基因组序列设计的向导RNA(gRNA)偶联的Cas9核酸内切酶，或与针对感兴趣基因组序列设计的gRNA偶联的Cpf1核酸内切酶，或与针对感兴趣基因组序列设计的gRNA偶联的Csm1核酸内切酶，来实现在感兴趣的铁氧还蛋白-硫氧还蛋白还原酶基因的上游插入增强子元件。或者，将与转录增强子元件融合的失活的核酸内切酶(例如，失活的Cas9、Cpf1或Csm1核酸内切酶)靶向感兴趣的铁氧还蛋白-硫氧还蛋白还原酶基因的转录起始位点附近的基因组位置，从而调节所述感兴趣的铁氧还蛋白-硫氧还蛋白还原酶基因的表达(Piatek等(2015)Plant Biotechnol J 13:578-589)。
铁氧还蛋白-硫氧还蛋白还原酶蛋白编码基因的表达的调节可通过采用精确基因组编辑技术以调节内源性序列的表达来实现。由此，通过使用本领域已知的方法将核酸插入编码铁氧还蛋白-硫氧还蛋白还原酶的天然植物序列附近。这类方法包括但不限于针对感兴趣植物基因组序列设计的大范围核酸酶(D’Halluin等，(2013)Plant Biotechnol J11:933-941)；CRISPR-Cas9，CRISPR-Cpf1，TALEN，和其他精确编辑基因组的技术(Feng等，(2013)Cell Research 23:1229-1232,Podevin等，(2013)Trends Biotechnology 31:375-383,Wei等，(2013)J Gen Genomics 40:281-289,Zhang等，(2013)WO 2013/026740，Zetsche等(2015)Cell 163:759-771,美国临时专利申请号62/295,325)；格里嗜盐碱杆菌属(N.gregoryi)亚尔古(Argonaute)-介导的DNA插入(Gao等(2016)Nat Biotechnol doi:10.1038/nbt.3547)；Cre-lox位点-特异性重组(Dale等，(1995)Plant J 7:649-659；Lyznik等，(2007)Transgenic Plant J 1:1-9；FLP-FRT重组(Li等，(2009)Plant Physiol151:1087-1095)；Bxb1-介导的整合(Yau等，Plant J(2011)701:147-166)；锌指介导的整合(Wright等，(2005)Plant J 44:693-705)；Cai等，(2009)Plant Mol Biol 69:699-709)；和同源重组(Lieberman-Lazarovich和Levy(2011)Methods Mol Biol 701:51-65)；Puchta,H.(2002)Plant Mol Biol48:173-182)。将利用所述核酸序列的插入来实现铁氧还蛋白-硫氧还蛋白还原酶基因的过表达、表达降低和/或表达谱改变的所需结果。
增强子包括能够在插入植物的基因组时增强基因表达的任何分子。因此，可将增强子插入位于感兴趣的铁氧还蛋白-硫氧还蛋白还原酶序列的上游或下游的基因组区中以增强表达。相对于表达将被增强的基因，增强子可以起顺式作用，并且可位于基因组内的任何位置。例如，增强子可位于其所增强表达的编码序列的约1Mbp以内、约100kbp以内、约50kbp、约30kbp、约20kbp、约10kbp、约5kbp、约3kbp、或约1kbp以内。增强子也可位于其所增强表达的基因的约1500bp以内，或者可紧邻或位于其所增强表达的基因的内含子以内。本发明的用于调节编码铁氧还蛋白-硫氧还蛋白还原酶蛋白或同源物的内源性基因表达的增强子包括经典增强子元件，如CaMV 35S增强子元件、巨细胞病毒(CMV)早期启动子增强子元件、和SV40增强子元件，还包括增强基因表达的内含子-介导的增强子元件如玉米shrunken-1增强子元件(Clancy和Hannah(2002)Plant Physiol.130(2):918-29)。可导入植物基因组以调节表达的增强子的其他示例包括PetE增强子(Chua等，(2003)Plant Cell15:11468-1479)，或水稻α-淀粉酶增强子(Chen等，(2002)J.Biol.Chem.277:13641-13649)，或本领域已知的任何增强子(Chudalayandi(2011)Methods Mol.Biol.701:285-300)。在一些实施方式中，本发明包括亚结构域、片段、或重复增强子元件(Benfrey等，(1990)EMBO J 9:1677-1684)。
铁氧还蛋白-硫氧还蛋白还原酶基因表达的改变还可通过以不改变DNA序列的方式来修饰该DNA而实现。此类改变可包括修饰感兴趣的铁氧还蛋白-硫氧还蛋白还原酶基因的染色质含量或结构和/或该铁氧还蛋白-硫氧还蛋白还原酶基因周围的DNA的染色质含量或结构。众所周知，染色质含量或结构中的此类变化能够影响基因转录(Hirschhorn等(1992)Genes and Dev 6:2288-2298；Narlikar等(2002)Cell 108:475-487)。此类改变可包括改变感兴趣的铁氧还蛋白-硫氧还蛋白还原酶基因的甲基化状态和/或感兴趣的铁氧还蛋白-硫氧还蛋白还原酶基因周围的DNA的甲基化状态。众所周知的是，DNA甲基化中的此类变化能够改变转录(Hsieh(1994)Mol Cell Biol 14:5487-5494)。经靶向的表观基因组编辑已被证明以可预测的方式影响基因的转录(Hilton等(2015)33:510-517)。本领域技术人员显见的是，可对DNA施用调节感兴趣的铁氧还蛋白-硫氧还蛋白还原酶基因的转录的其它类似改变(统称为“表观遗传学改变”)，以获得经改变的铁氧还蛋白-硫氧还蛋白还原酶基因表达谱的所需结果。
铁氧还蛋白-硫氧还蛋白还原酶基因表达的改变还可通过采用转座元件技术来改变基因表达而实现。众所周知，转座元件能够改变附近DNA的表达(McGinnis等(1983)Cell34:75-84)。编码铁氧还蛋白-硫氧还蛋白还原酶的基因的表达的改变可通过在感兴趣的铁氧还蛋白-硫氧还蛋白还原酶基因上游插入转座元件，造成所述基因的表达改变，来实现。
铁氧还蛋白-硫氧还蛋白还原酶基因表达的改变还可通过使调节感兴趣的铁氧还蛋白-硫氧还蛋白还原酶基因的表达的一种或多种转录因子表达来实现。应理解，转录因子表达的改变能够进而改变所述转录因子的靶基因的表达(Hiratsu等(2003)Plant J 34:733-739)。铁氧还蛋白-硫氧还蛋白还原酶基因表达的改变可以通过改变已知将与感兴趣的铁氧还蛋白-硫氧还蛋白还原酶基因相互作用的一种或多种转录因子的表达来实现。
铁氧还蛋白-硫氧还蛋白还原酶基因表达的改变还可通过在该感兴趣的植物物种中编码天然铁氧还蛋白-硫氧还蛋白还原酶的开放阅读框的上游插入启动子来实现。这将通过采用针对感兴趣的基因组序列设计的大范围核酸酶，在编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的开放阅读框的上游插入感兴趣的启动子来实现。该策略已知并且已经在先证实在预定位置处将转基因插入棉花基因组(D’Halluin等，(2013)Plant Biotechnol J 11:933-941)。对于本领域技术人员显而易见的是，通过在预定基因组基因座处引起双链断裂并提供适当的DNA模板用于插入，可以使用其他技术来实现在所述预定基因组基因座处插入遗传元件的类似结果(例如，CRISPR-Cas9，CRISPR-cpf1，CRISPR-Csm1，TALEN和用于精确编辑基因组的其他技术)。
通过说明的方式，而非限制性方式提供以下实施例。本说明书中涉及的所有专利申请和出版物指示本发明涉及领域技术人员的水平。所有发表物和专利申请通过引用纳入本文，就好像将各篇单独的发表物或专利申请具体和单独地通过引用纳入本文那样。
虽然出于方便理解的目的，通过阐述和举例的方式详细描述了上述发明，但可明显看出，某些改变和修改应属于所附权利要求书的范围。
实验部分
实施例1–铁氧还蛋白-硫氧还蛋白还原酶植物转化载体的构建
合成了编码玉米铁氧还蛋白-硫氧还蛋白还原酶蛋白的开放阅读框。该开放阅读框包含SEQ ID NO：1，编码SEQ ID NO：2的蛋白质序列。在编码序列的5'和3'末端包括适当的限制性位点，以允许将该DNA克隆到含有适于控制基因表达的遗传元件的植物转化载体中。在各植物转化构建体中，铁氧还蛋白-硫氧还蛋白还原酶开放阅读框位于植物启动子和5'非翻译区域(5'UTR)的下游和3'UTR的上游。表2总结了构建的含有铁氧还蛋白-硫氧还蛋白还原酶开放阅读框的植物转化构建体。
表2：铁氧还蛋白-硫氧还蛋白还原酶植物转化构建体
Figure BDA0002408987160000201
除了表2中列出的单基因铁氧还蛋白-硫氧还蛋白还原酶植物转化构建体外，还构建了含有铁氧还蛋白-硫氧还蛋白还原酶基因盒和第二连接盒的多基因植物转化构建体。表3总结了多基因铁氧还蛋白-硫氧还蛋白还原酶植物转化构建体。
表3：铁氧还蛋白-硫氧还蛋白还原酶多基因植物转化构建体
Figure BDA0002408987160000211
除了表2和3中描述的基因盒外，表2和3中列出的每种植物转化构建体还含有适于在引入植物转化载体后选择转化的植物细胞和植物再生的选择标记盒，如下所述。每个转化载体构建在质粒中，该质粒含有适于在大肠杆菌(E.coli)和根癌农杆菌(Agrobacteriumtumefaciens)中维持质粒的序列。在证实表2和3中列出的植物转化构建体含有所需序列后，将它们转化到根癌农杆菌(A.tumefaciens)细胞中用于植物转化。
实施例2-狗尾草(Setaria viridis)的转化
根据先前描述的方法(PCT/US2015/43989，通过引用并入本文)，使用携带铁氧还蛋白-硫氧还蛋白还原酶植物转化载体的根癌农杆菌(A.tumefaciens)细胞转化狗尾草(S.viridis)细胞。在用相关的植物转化载体转化狗尾草细胞并再生狗尾草植物后，进行PCR分析以证实狗尾草基因组中存在一个或多个感兴趣的基因。表4总结了用于转化狗尾草的转化构建体，以及用每种构建体转化产生的经PCR验证的转基因植物的数量。
表4：用铁氧还蛋白-硫氧还蛋白还原酶植物转化载体进行的狗尾草转化的总结
<u>构建体</u> <u>事件数量</u>
131361 37
131362 31
132307 9
132308 11
实施例3-玉米(Zea mays)的转化
携带铁氧还蛋白-硫氧还蛋白还原酶植物转化载体的根癌农杆菌(A.tumefaciens)细胞用于转化适于在组织培养基上再生的玉米(Zea mays cv.B104)细胞。在用相关植物转化载体转化玉米细胞并再生玉米植物后，进行PCR分析以确认玉米基因组中存在感兴趣的基因。
实施例4–水稻(Oryza sativa)的转化
携带铁氧还蛋白-硫氧还蛋白还原酶植物转化载体的根癌农杆菌(A.tumefaciens)细胞用于转化适于在组织培养基上再生的水稻(Oryza sativacv.Kitaake)细胞。在用相关植物转化载体转化水稻细胞并再生水稻植物后，进行PCR分析以确认水稻基因组中存在一个或多个感兴趣的基因。
实施例5-转基因狗尾草(S.viridis)的表征
在用铁氧还蛋白-硫氧还蛋白还原酶植物转化载体转化的狗尾草植物的转化和再生之后，将T0-代植物培养至成熟以产生T1-代种子。携带感兴趣的铁氧还蛋白-硫氧还蛋白还原酶基因盒的T1代狗尾草植物在温室环境中生长，以评估铁氧还蛋白-硫氧还蛋白还原酶基因表达对植物生长和最终地上生物质和种子产量的影响。随机化区组设计与野生型狗尾草边界行一起使用以消除分析中的边缘效应。空分离植物(Null segregant plant)在相同环境条件下在转基因狗尾草旁生长。表5总结了从由于转化而携带铁氧还蛋白-硫氧还蛋白还原酶基因盒的T1代狗尾草植物的实验所做的生物质和种子产量测定的结果。下表表示如表2中所述的用于转化的构建体，接着是从中收获T1种子的T0事件数量。
表5：用T1代植物进行的狗尾草温室观察的总结
<u>事件</u> <u>DW(g)</u> <u>种子产量(g)</u> <u>DW变化</u> <u>种子变化</u>
131361.12 3.35±0.30 0.90±0.13 -16.0％ -19.4％
131361.15 3.58±0.37 0.99±0.16 -10.3％ -11.4％
131361.18 4.32±0.14 1.15±0.08 8.3％ 2.3％
131361.21 4.08±0.46 1.06±0.14 2.3％ -5.2％
131361.23 4.00±0.32 1.14±0.12 0.4％ 2.2％
131361.空 3.99±0.13 1.12±0.07 n/a n/a
131362.10 3.59±0.21 1.04±0.11 5.2％ 11.2％
131362.24 4.18±0.20 1.29±0.09 22.4％ 37.4％
131362.29 2.59±0.48 0.60±0.15 -24.1％ -35.3％
131362.31 4.17±0.24 1.15±0.08 22.1％ 22.9％
131362.空 3.41±0.51 0.93±0.17 n/a n/a
132308-2 3.88±0.45 0.94±0.14 -9.3％ -21.0％
132308-3 4.33±0.71 1.41±0.07 1.2％ 18.5％
132308-6 4.46±0.15 1.42±0.07 4.2％ 19.3％
132308-空 4.28±0.12 1.19±0.07 n/a n/a
在表5中，地上生物质的干重在DW列中以克表示。类似地，收获的种子的干重在种子产量列中以克表示。DW变化和种子变化列分别表示相对于来自131220构建体的空分离植物的地上生物质和种子产量的变化百分比。如该表所示，来自131361构建体的5个事件中的3个显示出干重增加(0.4-8.3％增加)，而来自131361构建体的5个事件中的2个显示出相对于空对照增加的种子产量。来自131362构建体的4个测试的事件中的3个显示出干重和种子产量增加。来自132308构建体的3个测试的事件中的2个显示出干重和种子产量增加。
实施例6-转基因玉米的表征
用感兴趣的铁氧还蛋白-硫氧还蛋白还原酶植物转化载体转化的并经确认含有感兴趣基因的T0代玉米植物在温室中生长至成熟。当T0植物达到生殖阶段时，它们由适当的近交玉米品系授粉以产生杂交玉米种子。或者，或除了T0转基因玉米植物的授粉之外，来自T0的花粉用于授粉一个或多个近交玉米品系以产生杂交玉米种子。将由这些授粉产生的F1代杂交种子种植在两行或四行的田间环境中，并使用标准农艺实践进行培养。对植物进行基因分型以确定哪些植物含有以及哪些植物不含有铁氧还蛋白-硫氧还蛋白还原酶基因盒和铁氧还蛋白-硫氧还蛋白还原酶植物转化载体中包含的任何其他相关基因盒(例如，选择标记基因盒)。在玉米植物成熟后，收获种子。合并来自含有铁氧还蛋白-硫氧还蛋白还原酶基因盒的植物的种子，并且合并来自缺乏铁氧还蛋白-硫氧还蛋白还原酶基因盒的空分离植物的种子。称重种子，计算含有铁氧还蛋白-硫氧还蛋白还原酶基因盒的植物以及缺乏铁氧还蛋白-硫氧还蛋白还原酶基因盒的空分离植物的种子产量。进行适当的统计分析以确定含有铁氧还蛋白-硫氧还蛋白还原酶基因盒的植物是否比缺乏铁氧还蛋白-硫氧还蛋白还原酶基因盒的植物产生更高的产量。
或者，用感兴趣的铁氧还蛋白-硫氧还蛋白还原酶植物转化载体转化的并经确认含有感兴趣基因的T0代玉米植物在温室中生长至成熟，然后自花授粉。将得到的T1种子种植在温室中，并培养T1植物。对T1植物进行基因分型以鉴定纯合的、杂合的和空的分离植物。来自纯合T1植物的花粉用于授粉一个或多个近交玉米品系以产生杂交玉米种子。来自空分离植物的花粉也用于授粉一个或多个近交玉米品系以产生杂交玉米种子。将得到的杂交种子种植在两行或四行的田间环境，并使用标准农艺实践进行栽培。在玉米植物成熟后，收获种子。合并来自含有铁氧还蛋白-硫氧还蛋白还原酶基因盒的植物的种子，并且合并来自缺乏铁氧还蛋白-硫氧还蛋白还原酶基因盒的空分离植物的种子。称重种子，计算含有铁氧还蛋白-硫氧还蛋白还原酶基因盒的植物以及缺乏铁氧还蛋白-硫氧还蛋白还原酶基因盒的空分离植物的种子产量。进行适当的统计分析以确定含有铁氧还蛋白-硫氧还蛋白还原酶基因盒的植物是否比缺乏铁氧还蛋白-硫氧还蛋白还原酶基因盒的植物产生更高的产量。
实施例7-转基因水稻的表征
用感兴趣的铁氧还蛋白-硫氧还蛋白还原酶植物转化载体转化的并经确认含有感兴趣基因的T0代水稻植物在温室中生长至成熟，然后自花授粉。将得到的T1种子种植在温室中，并培养T1植物。对T1植物进行基因分型以鉴定纯合的、杂合的和空的分离植物。来自每组的植物生长至成熟并允许自花授粉以产生T2种子。收获由这种自花授粉产生的T2种子并称重，并计算纯合、杂合和空分离植物的种子产量。进行适当的统计分析以确定含有铁氧还蛋白-硫氧还蛋白还原酶基因盒的植物是否比缺乏铁氧还蛋白-硫氧还蛋白还原酶基因盒的植物产生更高的产量。
将T0代植物的自花授粉产生的种子所生长的T1代植物，或纯合T1代植物的自花授粉产生的种子所生长的T2代植物，在田间环境中生长。在T2代植物的情况下，空分离T1代植物也经自花授粉以产生T2代空植物作为阴性对照。使用标准农艺实践培育植物并使其成熟。成熟后，允许植物自花授粉。收获由这种自花授粉产生的种子并称重，计算纯合、杂合和空分离植物的种子产量。进行适当的统计分析以确定含有铁氧还蛋白-硫氧还蛋白还原酶基因盒的植物是否比缺乏铁氧还蛋白-硫氧还蛋白还原酶基因盒的植物产生更高的产量。
序列表
<110> 本森希尔生物系统股份有限公司(Benson Hill Biosystems, Inc.)
<120> 使用铁氧还蛋白-硫氧还蛋白还原酶增加植物生长和产量
<130> BHP016P
<160> 106
<170> PatentIn version 3.5
<210> 1
<211> 459
<212> DNA
<213> 玉米（Zea mays）
<220>
<221> misc_feature
<222> (1)..(459)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 1
atgacatcca ccgtcaccac aaccgttggg tgcggggggc tccccgtccg cccgttgtcg 60
acagcgacca gaggacgccc acgcagatgc gccgtccgag cccaggccgc gggagcggat 120
gcctccaatg ataagtcagt ggaggtcatg cgcaagttct ccgagcagta cgcccgccgc 180
tccaacactt tcttctgcgc cgacaagaca gtcactgccg tcgtcatcaa gggacttgct 240
gatcacaggg atactcttgg agctcctcta tgcccttgta ggcattatga tgacaaagct 300
gcggaggtag cacaaggatt ttggaattgc ccatgcgtcc ccatgcgtga gaggaaggaa 360
tgccactgta tgctttttct tactcccgat aatgactttg ctgggaagga tcaggttatc 420
tccttcgagg agatcaaaga ggcgacatcg aagttctaa 459
<210> 2
<211> 152
<212> PRT
<213> 玉米（Zea mays）
<220>
<221> MISC_FEATURE
<222> (1)..(152)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 2
Met Thr Ser Thr Val Thr Thr Thr Val Gly Cys Gly Gly Leu Pro Val
1 5 10 15
Arg Pro Leu Ser Thr Ala Thr Arg Gly Arg Pro Arg Arg Cys Ala Val
20 25 30
Arg Ala Gln Ala Ala Gly Ala Asp Ala Ser Asn Asp Lys Ser Val Glu
35 40 45
Val Met Arg Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Asn Thr Phe
50 55 60
Phe Cys Ala Asp Lys Thr Val Thr Ala Val Val Ile Lys Gly Leu Ala
65 70 75 80
Asp His Arg Asp Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr
85 90 95
Asp Asp Lys Ala Ala Glu Val Ala Gln Gly Phe Trp Asn Cys Pro Cys
100 105 110
Val Pro Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr
115 120 125
Pro Asp Asn Asp Phe Ala Gly Lys Asp Gln Val Ile Ser Phe Glu Glu
130 135 140
Ile Lys Glu Ala Thr Ser Lys Phe
145 150
<210> 3
<211> 780
<212> DNA
<213> 花椰菜花叶病毒（Cauliflower Mosaic Virus）
<220>
<221> 启动子
<222> (1)..(780)
<223> 2X35S 启动子
<400> 3
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 60
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 120
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 180
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 240
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 300
tcaaagcaag tggattgatg tgaacatggt ggagcacgac actctcgtct actccaagaa 360
tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat 420
atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt 480
agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca 540
agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga 600
aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga 660
cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag 720
ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa atctatctct 780
<210> 4
<211> 209
<212> DNA
<213> 花椰菜花叶病毒（Cauliflower Mosaic Virus）
<220>
<221> misc_feature
<222> (1)..(209)
<223> 35S 多聚A
<400> 4
gatctgtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt tcccagataa 60
gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta 120
tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc 180
cagtactaaa atccagatcc cccgaatta 209
<210> 5
<211> 798
<212> DNA
<213> 玉米（Zea mays）
<220>
<221> 启动子
<222> (1)..(798)
<223> RbcS7A 启动子+5'UTR
<400> 5
gatgactgat gacagacgtg gggaattcaa atgcaactct agcgaaagtt catatatttt 60
tcataaatag ctgaggctgg ggtaattatt ttttttgtag aaaaatagaa taggtggaat 120
ggttggggaa ggcgtaggcg ctcgtggacg acgcccgata aaagacaaga ggcggaattg 180
ccatgaattc gaggtagcta agtaaggcgc atatatatgc caaaaaattc tactgtcact 240
ttccaatttc aatgcgctgc caaacaagcc atcctggaaa ctgacttgaa ttcagcccaa 300
ttctgtagat ccaaacaggg ccggcgtcag tgcctcaggt gagagagcag cagacgatgc 360
aaagagccaa aactgcaagc agacgcagcc gaagccgaag ccgaagccca agcccaaaac 420
tgttttgtct ttgcccagaa ccgcgacgag cctaaactgc gcttcctcct atctacaagt 480
ccctggcaca tcacgcatag tccaaccatg gcgcgcaggc gataaggcga gccacgggga 540
cgcgacatgt ggtggcggac gcgatcagga tagggccagg ctggccgggc gcggccacgg 600
gatctagatg gccactcgtc ccacatccgc ttcgtcctgt cctgtactgc gtcctgcccc 660
caacgagagc cggagccggc catcccgtcg cacactctcc ccctctatat atgccgtcgg 720
tgtgggggag cctactacag gacgacccaa gcaagcaagc aagcagcgag tacatacata 780
ctaggcagcc aggcagcc 798
<210> 6
<211> 439
<212> DNA
<213> 玉米（Zea mays）
<220>
<221> 3'UTR
<222> (1)..(439)
<223> ZmRbcS 3'UTR
<400> 6
accgcgcccg ccggccgccc cccgccggct agctagctag ctagctcctg cgtgagctag 60
tagctagtgc catgcgtcgt ctctgtcgtt cggttttgct tcgggtcacc gtgtaccctt 120
tgcttgcttg gtttcttctt tccttttttc cttttttttt cttcttttcc ccggccatgg 180
ttcctttgct ttccagcagt tctctgctgg atgtgatgta tccattgttg caatcatggc 240
cttgcattgg ctacctctat acctgctaca aaactactgc aacgcctata tatacttggg 300
gtgaggaaca tgtgaatgca agctccggct atcatataca tgtaatatgg atacaaacta 360
tatatataaa tccgccgagg cgccgacaat actatacgac accgtgttaa gttaatatat 420
aactggtgct ttttattta 439
<210> 7
<211> 974
<212> DNA
<213> 玉米（Zea mays）
<220>
<221> 启动子
<222> (1)..(974)
<223> ZmRbcS 启动子+5'UTR
<400> 7
gagctccctt taatctggcg ctagatctgc atccgcggct tgcaaagata aatggcacat 60
ttagtgtgtt attttgcaat acctttcata gtagatatcc ttaaatgcag ttttaggcat 120
gtttgggtaa ttaaataaca tttttaggag gagttttaga tttacctttc tttcgtgatg 180
actgatgaca gacgtgggga attcaaatgc aactctagcg aaagttcata tatttttcat 240
aaatagctga ggctggggta attatttttt ttgtagaaaa atagaatagg tggaatggtt 300
ggggaaggcg taggcgctcg tggacgacgc ccgataaaag acaagaggcg gaattgccat 360
gaattcgagg tagctaagta aggcgcatat atatgccaaa aaattctact gtcactttcc 420
aatttcaatg cgctgccaaa caagccatcc tggaaactga cttgaattca gcccaattct 480
gtagatccaa acagggccgg cgtcagtgcc tcaggtgaga gagcagcaga cgatgcaaag 540
agccaaaact gcaagcagac gcagccgaag ccgaagccga agcccaagcc caaaactgtt 600
ttgtctttgc ccagaaccgc gacgagccta aactgcgctt cctcctatct acaagtccct 660
ggcacatcac gcatagtcca accatggcgc gcaggcgata aggcgcgcca cggggacgcg 720
acatgtggtg gcggacgcga tcaggatagg gccaggctgg ccgggcgcgg ccacgggaga 780
acggtggcca ctcgtcccac atccgcttcg tcctgtcctg tactgcgtcc tgcccccaac 840
gagagccgga gccggccatc ccgtcgcaca ctctccccct ctatatatgc cgtcggtgtg 900
ggggagccta ctacaggacg acccaagcaa gcaagcaagc agcgagtaca tacatactag 960
gcagccaggc agcc 974
<210> 8
<211> 1647
<212> DNA
<213> 聚球藻属（Synechococcus sp.）和豌豆（Pisum sativum）
<220>
<221> misc_feature
<222> (1)..(1647)
<223> RbcS-ictB
<220>
<221> 信号肽
<222> (1)..(339)
<223> 豌豆（P. sativum）RbcS信号肽
<220>
<221> misc_feature
<222> (340)..(1647)
<223> ictB
<400> 8
atggcttcta tgatttcttc ttctgctgtg acaacagtgt ctagggcttc taggggccag 60
tctgctgctg tggctccttt cggcggcctc aagtctatga caggcttccc tgtgaagaag 120
gtgaatacag atattacatc tattacatct aatggcggca gggtgaagtg catgcaggtg 180
tggcctccta ttggcaagaa gaagttcgag acactctctt acctccctcc tctcacaagg 240
gatatgacag tgtggcagac actcacattc gctcattacc agcctcagca gtggggccat 300
tcttctttcc tccataggct cttcggctct ctcagggctt ggagggcttc ttctcagctc 360
ctcgtgtggt ctgaggctct cggcggcttc ctcctcgctg tggtgtacgg ctctgctcct 420
ttcgtgcctt cttctgctct cggcctcggc ctcgctgcta ttgctgctta ctgggctctc 480
ctctctctca cagatattga tctcaggcag gctacaccta ttcattggct cgtgctcctc 540
tactggggcg tggatgctct cgctacaggc ctctctcctg tgagggctgc tgctctcgtg 600
ggcctcgcta agctcacact ctacctcctc gtgttcgctc tcgctgctag ggtgctcagg 660
aatcctaggc tcaggtctct cctcttctct gtggtggtga ttacatctct cttcgtgtct 720
gtgtacggcc tcaatcagtg gatttacggc gtggaggagc tcgctacatg ggtggatagg 780
aattctgtgg ctgatttcac atctagggtg tactcttacc tcggcaatcc taatctcctc 840
gctgcttacc tcgtgcctac aacagctttc tctgctgctg ctattggcgt gtggaggggc 900
tggctcccta agctcctcgc tattgctgct acaggcgctt cttctctctg cctcattctc 960
acatactcta ggggcggctg gctcggcttc gtggctatga ttttcgtgtg ggctctcctc 1020
ggcctctact ggttccagcc taggctccct gctccttgga ggaggtggct cttccctgtg 1080
gtgctcggcg gcctcgtggc tgtgctcctc gtggctgtgc tcggcctcga gcctctcagg 1140
gtgagggtgc tctctatttt cgtgggcagg gaggattctt ctaataattt caggattaat 1200
gtgtggctcg ctgtgctcca gatgattcag gataggcctt ggctcggcat tggccctggc 1260
aatacagctt tcaatctcgt gtaccctctc taccagcagg ctaggttcac agctctctct 1320
gcttactctg tgcctctcga ggtggctgtg gagggcggcc tcctcggcct cacagctttc 1380
gcttggctcc tcctcgtgac agctgtgaca gctgtgcgcc aggtgtctag gctcaggagg 1440
gataggaatc ctcaggcttt ctggctcatg gcttctctcg ctggcctcgc tggcatgctc 1500
ggccatggcc tcttcgatac agtgctctac aggcctgagg cttctacact ctggtggctc 1560
tgcattggcg ctattgcttc tttctggcag cctcagcctt ctaagcagct ccctcctgag 1620
gctgagcatt ctgatgagaa gatgtga 1647
<210> 9
<211> 548
<212> PRT
<213> 聚球藻属（Synechococcus sp.）和豌豆（Pisum sativum）
<220>
<221> MISC_FEATURE
<222> (1)..(548)
<223> RbcS-ictB
<220>
<221> MISC_FEATURE
<222> (1)..(113)
<223> 豌豆（P. sativum）RbcS信号肽
<220>
<221> MISC_FEATURE
<222> (114)..(548)
<223> ictB
<400> 9
Met Ala Ser Met Ile Ser Ser Ser Ala Val Thr Thr Val Ser Arg Ala
1 5 10 15
Ser Arg Gly Gln Ser Ala Ala Val Ala Pro Phe Gly Gly Leu Lys Ser
20 25 30
Met Thr Gly Phe Pro Val Lys Lys Val Asn Thr Asp Ile Thr Ser Ile
35 40 45
Thr Ser Asn Gly Gly Arg Val Lys Cys Met Gln Val Trp Pro Pro Ile
50 55 60
Gly Lys Lys Lys Phe Glu Thr Leu Ser Tyr Leu Pro Pro Leu Thr Arg
65 70 75 80
Asp Met Thr Val Trp Gln Thr Leu Thr Phe Ala His Tyr Gln Pro Gln
85 90 95
Gln Trp Gly His Ser Ser Phe Leu His Arg Leu Phe Gly Ser Leu Arg
100 105 110
Ala Trp Arg Ala Ser Ser Gln Leu Leu Val Trp Ser Glu Ala Leu Gly
115 120 125
Gly Phe Leu Leu Ala Val Val Tyr Gly Ser Ala Pro Phe Val Pro Ser
130 135 140
Ser Ala Leu Gly Leu Gly Leu Ala Ala Ile Ala Ala Tyr Trp Ala Leu
145 150 155 160
Leu Ser Leu Thr Asp Ile Asp Leu Arg Gln Ala Thr Pro Ile His Trp
165 170 175
Leu Val Leu Leu Tyr Trp Gly Val Asp Ala Leu Ala Thr Gly Leu Ser
180 185 190
Pro Val Arg Ala Ala Ala Leu Val Gly Leu Ala Lys Leu Thr Leu Tyr
195 200 205
Leu Leu Val Phe Ala Leu Ala Ala Arg Val Leu Arg Asn Pro Arg Leu
210 215 220
Arg Ser Leu Leu Phe Ser Val Val Val Ile Thr Ser Leu Phe Val Ser
225 230 235 240
Val Tyr Gly Leu Asn Gln Trp Ile Tyr Gly Val Glu Glu Leu Ala Thr
245 250 255
Trp Val Asp Arg Asn Ser Val Ala Asp Phe Thr Ser Arg Val Tyr Ser
260 265 270
Tyr Leu Gly Asn Pro Asn Leu Leu Ala Ala Tyr Leu Val Pro Thr Thr
275 280 285
Ala Phe Ser Ala Ala Ala Ile Gly Val Trp Arg Gly Trp Leu Pro Lys
290 295 300
Leu Leu Ala Ile Ala Ala Thr Gly Ala Ser Ser Leu Cys Leu Ile Leu
305 310 315 320
Thr Tyr Ser Arg Gly Gly Trp Leu Gly Phe Val Ala Met Ile Phe Val
325 330 335
Trp Ala Leu Leu Gly Leu Tyr Trp Phe Gln Pro Arg Leu Pro Ala Pro
340 345 350
Trp Arg Arg Trp Leu Phe Pro Val Val Leu Gly Gly Leu Val Ala Val
355 360 365
Leu Leu Val Ala Val Leu Gly Leu Glu Pro Leu Arg Val Arg Val Leu
370 375 380
Ser Ile Phe Val Gly Arg Glu Asp Ser Ser Asn Asn Phe Arg Ile Asn
385 390 395 400
Val Trp Leu Ala Val Leu Gln Met Ile Gln Asp Arg Pro Trp Leu Gly
405 410 415
Ile Gly Pro Gly Asn Thr Ala Phe Asn Leu Val Tyr Pro Leu Tyr Gln
420 425 430
Gln Ala Arg Phe Thr Ala Leu Ser Ala Tyr Ser Val Pro Leu Glu Val
435 440 445
Ala Val Glu Gly Gly Leu Leu Gly Leu Thr Ala Phe Ala Trp Leu Leu
450 455 460
Leu Val Thr Ala Val Thr Ala Val Arg Gln Val Ser Arg Leu Arg Arg
465 470 475 480
Asp Arg Asn Pro Gln Ala Phe Trp Leu Met Ala Ser Leu Ala Gly Leu
485 490 495
Ala Gly Met Leu Gly His Gly Leu Phe Asp Thr Val Leu Tyr Arg Pro
500 505 510
Glu Ala Ser Thr Leu Trp Trp Leu Cys Ile Gly Ala Ile Ala Ser Phe
515 520 525
Trp Gln Pro Gln Pro Ser Lys Gln Leu Pro Pro Glu Ala Glu His Ser
530 535 540
Asp Glu Lys Met
545
<210> 10
<211> 741
<212> DNA
<213> 野高粱（Sorghum bicolor）
<220>
<221> 启动子
<222> (1)..(741)
<223> 4xRGCGR 启动子
<400> 10
gaagcgagtg gcgcgctggc ggatgaggcg gcgagtggcc cggatgcacc ggcgcaggcg 60
agcgaagcga gtggcgcgct ggcggatgag gcggcgagtg gcccggatgc accggcgcag 120
gcgagcgaag cgagtggcgc gctggcggat gaggcggcga gtggcccgga tgcaccggcg 180
caggcgagcg aagcgagtgg cgcgctggcg gatgaggcgg cgagtggccc ggatgcaccg 240
gcgcaggcga gccgcacgcc gccgcccgcc gcggcgctcg cgcgcgcacc gctgccgcct 300
gccgccacac aatgcgagcg cgcgcgcaca cacacacaca ccacccgggc gggggggctg 360
tagtagtaac ggccttgtct tgtcggcacg cgcgcgtccg tgtgtataag gaggcaggcc 420
cgcgacaacg ataagcggca ctcgcacgat caatgtacac attgcccgtc cgcgccacca 480
catccagcat cgtcgccagc ctcgccaccc ccgcgccgtc ctcctcctcc ggctccggct 540
ccggccgccc caggcccagg ctcatccgga acgcccccgt cttcgccgcc cccgccaccg 600
tcgtgtaaac gggacggcgg gcagctgagg agtcaaacga gagagatcga gagaaagaaa 660
gggagggcat ccaccagccg ccggcgataa gaggggagga gagagaggcc agagaagagg 720
aggagaagaa gaagaaatcg a 741
<210> 11
<211> 525
<212> DNA
<213> 玉米（Zea mays）
<220>
<221> misc_feature
<222> (1)..(525)
<223> GRMZM2G181258
<400> 11
atggccctcg agacatgctt tagggcctgg gccctccacg ccgcgccagc cgggtccaag 60
gaccgcctcc tcgtgtgttc ctccgggggg aacctcgtcc tgccgtccaa gagggtcgcg 120
gccgcgccac tctccgtcgg cagggtcgcg acccgccgcg cccgccatgt gtgccagtcc 180
aaaaatgcgg tcgatgaagt gctcgtcgcg gatgaaaaaa actgggatgg catggtcatg 240
gcgtgtgaga ccccagtgct ggtcgaattc tgggccccat ggtgtgggcc gtgccgcatg 300
attgcgccgg tcatcgacga gctggcgaag gactatgcgg gcaaaattat gtgttgcaaa 360
gtcaatacag acgacagccc gaatgtcgcg tccacctacg gcattaggtc catcccaaca 420
gtgctcatct ttaaaggcgg cgagaagaaa gagagcgtca ttggcgcggt gccaaaaagc 480
acactcacaa ccctcatcga caaatatatc gggtccagct cctga 525
<210> 12
<211> 174
<212> PRT
<213> 玉米（Zea mays）
<220>
<221> MISC_FEATURE
<222> (1)..(174)
<223> GRMZM2G181258
<400> 12
Met Ala Leu Glu Thr Cys Phe Arg Ala Trp Ala Leu His Ala Ala Pro
1 5 10 15
Ala Gly Ser Lys Asp Arg Leu Leu Val Cys Ser Ser Gly Gly Asn Leu
20 25 30
Val Leu Pro Ser Lys Arg Val Ala Ala Ala Pro Leu Ser Val Gly Arg
35 40 45
Val Ala Thr Arg Arg Ala Arg His Val Cys Gln Ser Lys Asn Ala Val
50 55 60
Asp Glu Val Leu Val Ala Asp Glu Lys Asn Trp Asp Gly Met Val Met
65 70 75 80
Ala Cys Glu Thr Pro Val Leu Val Glu Phe Trp Ala Pro Trp Cys Gly
85 90 95
Pro Cys Arg Met Ile Ala Pro Val Ile Asp Glu Leu Ala Lys Asp Tyr
100 105 110
Ala Gly Lys Ile Met Cys Cys Lys Val Asn Thr Asp Asp Ser Pro Asn
115 120 125
Val Ala Ser Thr Tyr Gly Ile Arg Ser Ile Pro Thr Val Leu Ile Phe
130 135 140
Lys Gly Gly Glu Lys Lys Glu Ser Val Ile Gly Ala Val Pro Lys Ser
145 150 155 160
Thr Leu Thr Thr Leu Ile Asp Lys Tyr Ile Gly Ser Ser Ser
165 170
<210> 13
<211> 287
<212> DNA
<213> 玉米（Zea mays）
<220>
<221> 3'UTR
<222> (1)..(287)
<223> ZmCA1 3'UTR
<400> 13
gttcaaaact agggctacgg caattctacc ggcccgccga ctcctgcatc atcataaata 60
tatatactat actatactac tacgtaccta ccgatatgca cccgagcaat gtgaatgcgt 120
cgagtactat atatctgttt tctgcatcta catatatata ccggatcaat cgcccaatgt 180
gaatgtaata agcaatatca ttttctacca cttttcattc ctaacgctga gctttttatg 240
tactatatct tatatgatga ataataatat gaccgccttg tgatcta 287
<210> 14
<211> 154
<212> PRT
<213> 野高粱（Sorghum bicolor）
<220>
<221> MISC_FEATURE
<222> (1)..(154)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 14
Met Thr Ser Thr Val Thr Thr Thr Val Gly Cys Gly Gly Leu Pro Val
1 5 10 15
Arg Pro Ser Ser Ser Ser Thr Ala Arg Arg Gly Arg Pro Arg Arg Cys
20 25 30
Ala Val Arg Ala Gln Ala Ala Gly Ala Asp Ala Ser Asn Asp Lys Ser
35 40 45
Val Glu Val Met Arg Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Asn
50 55 60
Thr Phe Phe Cys Ala Asp Lys Thr Val Thr Ala Val Val Ile Lys Gly
65 70 75 80
Leu Ala Asp His Arg Asp Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg
85 90 95
His Tyr Asp Asp Lys Ala Ala Glu Val Ala Gln Gly Phe Trp Asn Cys
100 105 110
Pro Cys Val Pro Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe
115 120 125
Leu Thr Pro Asp Asn Asp Phe Ala Gly Lys Asp Gln Val Ile Ser Leu
130 135 140
Glu Glu Ile Lys Glu Ala Thr Ser Lys Phe
145 150
<210> 15
<211> 153
<212> PRT
<213> 小米（Setaria italica）
<220>
<221> MISC_FEATURE
<222> (1)..(153)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 15
Met Thr Ser Ala Val Ala Thr Thr Val Gly Cys Gly Gly Leu Pro Phe
1 5 10 15
Arg Pro Ser Ser Ala Ala Pro Arg Gly Arg Pro Arg Gly Arg Trp Met
20 25 30
Val Arg Ala Gln Ala Ala Gly Ala Asp Ala Ser Asp Asp Lys Ser Leu
35 40 45
Glu Ile Met Arg Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Asn Thr
50 55 60
Phe Phe Cys Ala Asp Lys Ser Val Thr Ala Val Val Ile Lys Gly Leu
65 70 75 80
Ala Asp His Arg Asp Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His
85 90 95
Tyr Asp Asp Lys Ala Ala Glu Val Ala Gln Gly Phe Trp Asn Cys Pro
100 105 110
Cys Val Pro Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu
115 120 125
Thr Pro Asp Asn Asp Phe Ala Gly Gln Asp Gln Ala Ile Ser Leu Glu
130 135 140
Glu Ile Lys Glu Ala Thr Ser Lys Phe
145 150
<210> 16
<211> 152
<212> PRT
<213> 双稃草（Diplachne fusca）
<220>
<221> MISC_FEATURE
<222> (1)..(152)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 16
Met Leu Pro Thr Val Ala Ala Thr Val Gly Ser Gly Val Leu Pro Val
1 5 10 15
Arg Pro Ser Ser Thr Ala Pro Ser Gly Arg Ser Arg Arg Phe Ala Val
20 25 30
Arg Ala Gln Ala Glu Ala Ala Gly Asp Pro Gly Asp Lys Ser Val Glu
35 40 45
Ile Met Arg Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Asn Thr Tyr
50 55 60
Phe Cys Ala Asp Lys Thr Val Thr Ala Val Val Ile Lys Gly Leu Ala
65 70 75 80
Asp His Lys Asp Gln Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr
85 90 95
Asp Asp Lys Ala Ala Glu Val Ala Gln Gly Phe Trp Asn Cys Pro Cys
100 105 110
Val Pro Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr
115 120 125
Pro Glu Asn Asp Phe Ala Gly Gln Asp Gln Ala Ile Thr Phe Glu Glu
130 135 140
Ile Lys Glu Ala Thr Ser Lys Phe
145 150
<210> 17
<211> 149
<212> PRT
<213> 二穗短柄草（Brachypodium distachyon）
<220>
<221> MISC_FEATURE
<222> (1)..(149)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 17
Met Ser Ser Ser Phe Thr Thr Thr Ala Val Arg Ser Pro Leu Leu Cys
1 5 10 15
Pro Ile Ser Thr Ser Ala Ala Gly Leu Arg Arg Arg Ala Val Arg Ala
20 25 30
Gln Ala Gly Gly Val Asp Ser Ser Asp Lys Ser Val Glu Ile Met Arg
35 40 45
Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Ser Thr Phe Phe Cys Ser
50 55 60
Asp Lys Ser Val Thr Ala Val Val Ile Lys Gly Leu Ala Asp His Lys
65 70 75 80
Asp Gln Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys
85 90 95
Ala Ala Glu Ala Ala Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met
100 105 110
Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn
115 120 125
Asp Phe Ala Gly Glu Asp Gln Ala Ile Ser Leu Asp Glu Ile Lys Glu
130 135 140
Ala Thr Ser Lys Phe
145
<210> 18
<211> 157
<212> PRT
<213> 山羊草节节麦亚种（Aegilops tauschii subsp. Tauschii）
<220>
<221> MISC_FEATURE
<222> (1)..(157)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 18
Met Met Ser Ile Thr Ser Thr Ala Ala Ala Gly Thr Pro Leu Cys Arg
1 5 10 15
Pro Ser Val Ser Arg Gly Gly Arg Arg Ser Arg Cys Ala Val Arg Ala
20 25 30
Gln Ala Ala Gly Asp Gly Gly Ala Ala Ala Asp Gly Ala Ser Ser Glu
35 40 45
Gln Lys Ser Leu Glu Ile Met Arg Lys Phe Ser Glu Gln Tyr Ala Arg
50 55 60
Arg Ser Ser Thr Phe Phe Cys Ser Asp Lys Ser Val Thr Ala Val Val
65 70 75 80
Ile Lys Gly Leu Ala Asp His Lys Asp Gln Leu Gly Ala Pro Leu Cys
85 90 95
Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala Ala Gln Gly Phe
100 105 110
Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu Cys His Cys
115 120 125
Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Glu Asp Gln Ala
130 135 140
Ile Ser Leu Glu Glu Ile Lys Glu Ala Thr Ser Lys Tyr
145 150 155
<210> 19
<211> 155
<212> PRT
<213> 短花药野生稻（Oryza brachyantha）
<220>
<221> MISC_FEATURE
<222> (1)..(155)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 19
Met Met Ser Met Ser Ser Thr Ala Ala Ala Ser Pro Phe Leu Thr Ser
1 5 10 15
Pro Met Pro Ser Pro Ser Thr Ser Thr Gly Leu Arg Lys Cys Phe Val
20 25 30
Arg Pro Arg Ala Gln Ala Gly Ser Gly Gly Ala Asp Ala Ser Asp Lys
35 40 45
Ser Ile Glu Ile Met Arg Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser
50 55 60
Asn Thr Phe Phe Cys Ser Glu Lys Ser Val Thr Ala Val Val Ile Lys
65 70 75 80
Gly Leu Ala Asp His Lys Asp Gln Leu Gly Ala Pro Leu Cys Pro Cys
85 90 95
Arg His Tyr Asp Asp Lys Ala Ala Glu Val Ala Gln Gly Phe Trp Asn
100 105 110
Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu Cys His Cys Met Leu
115 120 125
Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Glu Asp Gln Ala Ile Thr
130 135 140
Leu Glu Glu Ile Lys Asp Ala Thr Ser Lys Ile
145 150 155
<210> 20
<211> 146
<212> PRT
<213> 小果野蕉（Musa acuminata）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 20
Met Ser Ile Gln Thr Ala Phe Arg Gly Val Ala Leu Pro Ser Leu Thr
1 5 10 15
Pro Ala Ser Ser Ser Pro His Arg Pro Pro Arg Phe Met Ile Arg Ala
20 25 30
Glu Val Glu Pro Ser Asp Lys Ser Val Glu Val Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly
50 55 60
Val Thr Ala Val Val Ile Lys Gly Leu Ala Asp His Arg Asp Ser Val
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu
85 90 95
Val Ala Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Thr Glu Gln Ala Ile Ser Phe Glu Glu Ile Lys Glu Thr Thr Ser
130 135 140
Lys Ile
145
<210> 21
<211> 146
<212> PRT
<213> 水稻（Oryza sativa）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 21
Met Met Ser Met Ala Ser Thr Thr Ala Ser Pro Phe Cys Pro Ser Pro
1 5 10 15
Met Pro Arg Gly Arg Lys Cys Thr Val Arg Val Gln Ala Gly Ala Ala
20 25 30
Gly Ala Asp Ala Ser Asp Lys Ser Leu Glu Ile Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Arg Ser Asn Thr Phe Phe Cys Ser Glu Lys Ser
50 55 60
Val Thr Ala Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Gln Leu
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu
85 90 95
Val Ala Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Gln Asp Gln Ala Ile Thr Leu Glu Glu Ile Lys Asp Ala Thr Ser
130 135 140
Lys Ile
145
<210> 22
<211> 146
<212> PRT
<213> 水稻（Oryza sativa）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 22
Met Met Leu Met Ala Ser Thr Thr Ala Ser Pro Phe Cys Pro Ser Pro
1 5 10 15
Met Pro Arg Gly Arg Lys Cys Thr Val Arg Val Gln Ala Gly Ala Ala
20 25 30
Gly Ala Asp Ala Ser Asp Lys Ser Leu Glu Ile Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Arg Ser Asn Thr Phe Phe Cys Ser Glu Lys Ser
50 55 60
Val Thr Ala Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Gln Leu
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu
85 90 95
Val Ala Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Gln Asp Gln Ala Ile Thr Leu Glu Glu Ile Lys Asp Ala Thr Ser
130 135 140
Lys Ile
145
<210> 23
<211> 201
<212> PRT
<213> 单囊二型花（Dichanthelium oligosanthes）
<220>
<221> MISC_FEATURE
<222> (1)..(201)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 23
Met Thr Ser Thr Val Ala Thr Thr Val Gly Cys Gly Gly Leu Pro Phe
1 5 10 15
Arg Pro Ser Ser Thr Ala Pro Arg Pro Arg Gly Arg Trp Val Val Arg
20 25 30
Ala Gln Ala Ala Gly Ala Asp Ala Ser Asp Asp Lys Ser Val Glu Ile
35 40 45
Met Arg Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Asn Thr Phe Phe
50 55 60
Cys Ala Asp Lys Ser Val Thr Ala Val Val Ile Lys Gly Leu Ala Asp
65 70 75 80
His Lys Asp Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp
85 90 95
Asp Lys Ala Ala Glu Ala Ala Gln Gly Phe Trp Asn Cys Pro Cys Val
100 105 110
Pro Met Arg Glu Arg Cys Val Cys Glu Leu Tyr Phe Arg Leu Phe Phe
115 120 125
Met Met Val His Arg Glu Asn Val Ala Met Ser Leu Phe Tyr Val Gln
130 135 140
Ser Ser Ala Val Leu Leu Gln Tyr Arg Lys His His Phe Leu Cys Glu
145 150 155 160
Trp Ser Thr Phe Ala Gly Lys Asn Ala Thr Val Cys Phe Ser Leu Leu
165 170 175
Gln Thr Met Ile Ser Leu Glu Arg Thr Arg His Val Ile Ser Leu Asp
180 185 190
Glu Ile Lys Glu Ala Thr Ser Lys Phe
195 200
<210> 24
<211> 147
<212> PRT
<213> 蔓花生（Arachis duranensis）
<220>
<221> MISC_FEATURE
<222> (1)..(147)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 24
Met Thr Ser Gln Ala Ser Ser Ser Ala Ala Ala Phe Ala Leu Pro Val
1 5 10 15
Ser Ser Ala Thr Ser Ser Ile Arg Arg Arg Asn Pro Thr Leu Val Arg
20 25 30
Ala Gln Val Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe
35 40 45
Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys
50 55 60
Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser
65 70 75 80
Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala
85 90 95
Glu Ala Ala Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu
100 105 110
Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe
115 120 125
Ala Gly Lys Glu Gln Ala Ile Thr Leu Asp Glu Ile Lys Glu Ala Thr
130 135 140
Ala Asn Met
145
<210> 25
<211> 148
<212> PRT
<213> 艾花生（Arachis ipaensis）
<220>
<221> MISC_FEATURE
<222> (1)..(148)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 25
Met Thr Ser His Ala Ser Ser Ser Ala Ala Ala Ala Phe Ala Leu Pro
1 5 10 15
Val Ser Ser Ala Thr Ser Ser Ile Arg Arg Arg Asn Pro Thr Leu Val
20 25 30
Arg Ala Gln Val Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys
35 40 45
Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp
50 55 60
Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp
65 70 75 80
Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala
85 90 95
Ala Glu Ala Ala Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp
115 120 125
Phe Ala Gly Lys Glu Gln Ala Ile Thr Leu Asp Glu Ile Lys Glu Ala
130 135 140
Thr Ala Asn Met
145
<210> 26
<211> 144
<212> PRT
<213> 野大豆（Glycine soja）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 26
Met Thr Thr Gln Ala Ser Thr Phe Ala Val Ala Val Pro Ser Val Thr
1 5 10 15
Thr Pro Phe Arg Ser His Arg Asn Pro Phe Val Val Arg Ala Gln Ala
20 25 30
Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val Thr
50 55 60
Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Thr Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val Ala
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Asn
115 120 125
Glu Gln Thr Ile Thr Leu Asp Glu Ile Lys Glu Ser Thr Ala Asn Met
130 135 140
<210> 27
<211> 145
<212> PRT
<213> 油棕（Elaeis guineensis）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 27
Met Ser Thr Gln Thr Ala Leu His Gly Ile Pro Leu Pro Ile Phe Asn
1 5 10 15
Leu Ala Ser Ser Pro Ser Arg Ser His Arg Leu Val Val Arg Ala Lys
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Lys Tyr Ala Arg Gln Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val
85 90 95
Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Asn Pro Asp Asn Asp Phe Ala Gly
115 120 125
Thr Glu Gln Thr Ile Ser Leu Glu Glu Ile Lys Glu Ala Thr Ser Lys
130 135 140
Ile
145
<210> 28
<211> 145
<212> PRT
<213> 巨桉(Eucalyptus grandis)
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 28
Met Thr Leu Gln Ala Ser Ser Phe Ala Ala Val Gly Leu Pro Ser Leu
1 5 10 15
Ala Pro Arg Leu Cys Arg Ser Arg Arg Gly Pro Val Val Arg Ala Lys
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Thr Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Pro Ala Glu Val
85 90 95
Ala Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Glu Asn Asp Phe Ala Gly
115 120 125
Gln Asp Gln Ala Ile Ser Phe Glu Glu Ile Lys Glu Ser Thr Ser Asn
130 135 140
Met
145
<210> 29
<211> 145
<212> PRT
<213> 绿豆（Vigna radiata）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 29
Met Thr Thr Gln Ala Ser Ala Phe Ser Leu Ala Ala Pro Ser Val Ala
1 5 10 15
Thr Pro Phe Arg Gly Arg Asn Arg Asn Pro Leu Leu Val Arg Ala Arg
20 25 30
Val Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala
85 90 95
Ala Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Asp Glu Gln Val Ile Thr Leu Glu Glu Ile Lys Glu Ser Thr Ala Asn
130 135 140
Met
145
<210> 30
<211> 150
<212> PRT
<213> 小果野蕉（Musa acuminata）
<220>
<221> MISC_FEATURE
<222> (1)..(150)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 30
Met Ala Leu Ser Pro Arg Thr Ala Ala Leu His Gly Val Ala Pro Leu
1 5 10 15
Pro Arg Leu Arg Leu Pro Ser Leu Thr Ser Cys Gly Ser Arg Arg Val
20 25 30
Asn Val Arg Ala Lys Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met
35 40 45
Arg Lys Phe Ser Glu Gln Tyr Ala Arg Lys Ser Glu Thr Tyr Phe Cys
50 55 60
Ile Asp Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His
65 70 75 80
Lys Asp Met Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp
85 90 95
Lys Ala Ala Glu Val Ala Gln Gly Phe Trp Asn Cys Pro Cys Val Pro
100 105 110
Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Asn Pro Asp
115 120 125
Asn Asp Phe Ala Gly Lys Glu Gln Ser Ile Thr Leu Glu Glu Ile Lys
130 135 140
Glu Ala Ile Ser Gln Ile
145 150
<210> 31
<211> 144
<212> PRT
<213> 大豆（Glycine max）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 31
Met Thr Thr Gln Ala Ser Thr Phe Ala Val Ala Val Pro Ser Val Ala
1 5 10 15
Thr Pro Phe Arg Arg His Arg Asn Pro Phe Val Val Arg Ala Gln Ala
20 25 30
Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val Thr
50 55 60
Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Thr Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val Ala
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Asn
115 120 125
Glu Gln Thr Ile Thr Leu Asp Glu Ile Lys Glu Ser Thr Ala Asn Met
130 135 140
<210> 32
<211> 145
<212> PRT
<213> 白花羽扇豆（Lupinus angustifolius）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 32
Met Ser Leu Gln Ala Ser Thr Phe Ala Val Ala Ile Pro Phe Ser Thr
1 5 10 15
Ala Thr Pro Leu Arg Arg His Arg Asn Leu Phe Thr Val Arg Ala Gln
20 25 30
Val Glu Pro Ser Asp Lys Asn Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala
85 90 95
Ser Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Glu Gln Thr Ile Thr Leu Glu Glu Ile Lys Glu Ala Thr Ala Asn
130 135 140
Met
145
<210> 33
<211> 147
<212> PRT
<213> 菠萝（Ananas comosus）
<220>
<221> MISC_FEATURE
<222> (1)..(147)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 33
Met Ser Ser Gln Thr Leu Leu Leu Gly Ala Ala Phe Pro Ser Pro Ala
1 5 10 15
Leu Ala Leu Ala Pro Ser Arg Ser Arg Ser Arg Arg Leu Arg Ile Arg
20 25 30
Ala Gln Val Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe
35 40 45
Ser Glu Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Met Asp Lys
50 55 60
Gly Val Thr Ala Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser
65 70 75 80
Ile Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala
85 90 95
Glu Val Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu
100 105 110
Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Glu Asn Asp Phe
115 120 125
Ala Gly Lys Glu Gln Ala Ile Thr Leu Glu Glu Ile Lys Glu Ala Thr
130 135 140
Ser Lys Leu
145
<210> 34
<211> 145
<212> PRT
<213> 醉蝶花（Tarenaya hassleriana）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 34
Met Asn Leu Gln Ala Ala Ser Cys Ser Phe Ser Ser Val Trp Val Pro
1 5 10 15
Leu Ala Ser Pro Arg Thr Ser Ser Arg Arg Ser Val Ile Arg Ala Lys
20 25 30
Ser Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ala Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser His Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val
85 90 95
Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Lys Asp Gln Ala Ile Ser Leu Glu Glu Ile Asn Glu Ala Thr Ala Asn
130 135 140
Met
145
<210> 35
<211> 146
<212> PRT
<213> 海枣（Phoenix dactylifera）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 35
Met Ser Thr Gln Thr Ala Ala Phe His Gly Val Pro Leu Pro Ile Phe
1 5 10 15
Gln Pro Ala Ser Pro His Pro Arg Pro Arg Arg Leu Leu Val Arg Ala
20 25 30
Lys Val Glu Pro Ser Glu Lys Ser Val Glu Leu Met Arg Arg Phe Ser
35 40 45
Glu Lys Tyr Ala Arg Gln Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly
50 55 60
Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser His
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ser Ala Glu
85 90 95
Val Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Lys Glu Gln Ala Ile Thr Leu Glu Glu Ile Lys Glu Ala Thr Ser
130 135 140
His Val
145
<210> 36
<211> 149
<212> PRT
<213> 江南卷柏（Selaginella moellendorffii）
<220>
<221> MISC_FEATURE
<222> (1)..(149)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 36
Met Ala Ala Met Ala Ser Ile Ala Thr Thr Ser Arg Asn Leu Gly Gly
1 5 10 15
Ala Phe Val Asn Pro Ser Ser Thr Pro Arg Arg Arg Ile His Val Val
20 25 30
Arg Ala Ser Ser Glu Pro Ser Ser Lys Ser Ile Glu Asn Met Arg Lys
35 40 45
Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Ala Asp
50 55 60
Lys Ser Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp
65 70 75 80
Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Pro
85 90 95
Ala Glu Ala Ser Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Glu Asn Asp
115 120 125
Phe Ala Gly Lys Asp Gln Thr Ile Ser Ala Asp Glu Ile Lys Asp Leu
130 135 140
Thr Ala Ser Leu Gly
145
<210> 37
<211> 154
<212> PRT
<213> 白花羽扇豆（Lupinus angustifolius）
<220>
<221> MISC_FEATURE
<222> (1)..(154)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 37
Met Ser Leu Gln Ala Ser Thr Phe Ala Val Ala Ile Pro Phe Ser Thr
1 5 10 15
Ala Thr Pro Leu Arg Arg His Arg Asn Leu Phe Thr Val Arg Ala Gln
20 25 30
Asp Gly Asn Asn Leu Leu Val Leu Ala Val Glu Pro Ser Asp Lys Asn
35 40 45
Val Glu Ile Met Arg Lys Phe Ser Glu Gln Tyr Ala Arg Lys Ser Gly
50 55 60
Thr Tyr Phe Cys Val Asp Lys Gly Val Thr Ser Val Val Ile Lys Gly
65 70 75 80
Leu Ala Asp His Lys Asp Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg
85 90 95
His Tyr Asp Asp Lys Ala Ala Glu Ala Ser Gln Gly Phe Trp Asn Cys
100 105 110
Pro Cys Val Pro Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe
115 120 125
Leu Thr Pro Asp Asn Asp Phe Ala Gly Gln Glu Gln Thr Ile Thr Leu
130 135 140
Glu Glu Ile Lys Glu Ala Thr Ala Asn Met
145 150
<210> 38
<211> 140
<212> PRT
<213> 大豆（Glycine max）
<220>
<221> MISC_FEATURE
<222> (1)..(140)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 38
Met Thr Thr Gln Ala Ser Thr Phe Ala Val Ala Val Pro Ser Val Thr
1 5 10 15
Thr Pro Phe Arg Ser His Arg Asn Pro Phe Val Val Arg Ala Gln Asp
20 25 30
Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln Tyr Ala Arg Lys
35 40 45
Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val Thr Ser Val Val Ile
50 55 60
Lys Gly Leu Ala Asp His Lys Asp Thr Leu Gly Ala Pro Leu Cys Pro
65 70 75 80
Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val Ala Gln Gly Phe Trp
85 90 95
Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu Cys His Cys Met
100 105 110
Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Asn Glu Gln Thr Ile
115 120 125
Thr Leu Asp Glu Ile Lys Glu Ser Thr Ala Asn Met
130 135 140
<210> 39
<211> 149
<212> PRT
<213> 胡杨（Populus euphratica）
<220>
<221> MISC_FEATURE
<222> (1)..(149)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 39
Met Asn Trp Ser Cys Ser Thr Ala Thr Thr Cys Gly Ile Ser Ser Ser
1 5 10 15
Ser Ser Cys Phe Ser Cys Pro Pro Lys Thr Pro Phe Arg Ala Val Ile
20 25 30
Arg Ala Ser Lys Val Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg
35 40 45
Lys Phe Ser Glu Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val
50 55 60
Asp Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys
65 70 75 80
Asp Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys
85 90 95
Ala Ala Glu Ala Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met
100 105 110
Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn
115 120 125
Asp Phe Ala Gly Lys Asp Gln Thr Ile Ser Leu Glu Glu Ile Arg Glu
130 135 140
Ser Thr Ala Asn Met
145
<210> 40
<211> 148
<212> PRT
<213> 烟草（Nicotiana tabacum）
<220>
<221> MISC_FEATURE
<222> (1)..(148)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 40
Met Thr Thr Leu Gln Ala Ser Thr Ser Tyr Ser Val Gly Phe Gly Ile
1 5 10 15
Ser Ser Phe Ala Thr Leu Pro Lys Ser Ser Ser Arg Arg Tyr Val Thr
20 25 30
Val Ala Lys Met Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys
35 40 45
Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Met Asp
50 55 60
Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp
65 70 75 80
Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala
85 90 95
Ala Glu Ala Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp
115 120 125
Phe Ala Gly Glu Glu Gln Ala Ile Ser Met Glu Glu Ile Lys Glu Thr
130 135 140
Thr Ala Asn Met
145
<210> 41
<211> 144
<212> PRT
<213> 鹰嘴豆（Cicer arietinum）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 41
Met Thr Thr Gln Ala Ser Thr Phe Ala Phe Ser Val Ser Ser Val Ala
1 5 10 15
Ser Pro Leu Arg Arg Arg Arg Asn Pro Thr Val Val Arg Ala Gln Val
20 25 30
Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Lys Ser Glu Thr Phe Phe Cys Val Asp Lys Gly Val Thr
50 55 60
Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala Ala
85 90 95
His Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Asn
115 120 125
Glu Gln Thr Ile Thr Leu Asp Glu Ile Lys Glu Ser Thr Ala Asn Met
130 135 140
<210> 42
<211> 150
<212> PRT
<213> 北美云杉（Picea sitchensis）
<220>
<221> MISC_FEATURE
<222> (1)..(150)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 42
Met Ala Thr Ser Val Ile Asn Thr Ser Ile Ser Asn Leu Gly Val Ser
1 5 10 15
Ser Leu Lys Ser Ser Ala Asn Ala Arg Ile Glu Ser Lys Tyr Ser Met
20 25 30
Gln Val Arg Ala Lys Val Glu Pro Ser Asn Lys Ser Val Glu Ile Met
35 40 45
Arg Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Asp Thr Tyr Phe Cys
50 55 60
Val Asp Lys Ala Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His
65 70 75 80
Lys Asp Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp
85 90 95
Lys Val Ala Glu Ala Glu Gln Gly Phe Trp Asn Cys Pro Cys Val Pro
100 105 110
Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Thr Asp
115 120 125
Asn Asp Phe Ser Gly Lys Glu Gln Thr Ile Ser Ser Glu Glu Ile Lys
130 135 140
Asp Leu Thr Ala Lys Phe
145 150
<210> 43
<211> 144
<212> PRT
<213> 地三叶（Trifolium subterraneum）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 43
Met Thr Ser Gln Ala Ser Thr Phe Ala Val Ser Val Ser Ser Val Ala
1 5 10 15
Ser Pro Leu Arg Arg Arg Arg Asn Phe His Val Val Arg Ala Gln Val
20 25 30
Glu Pro Ser Asp Lys Ser Ile Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Ser Asp Lys Gly Val Thr
50 55 60
Ala Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala Ala
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Asn
115 120 125
Glu Gln Thr Ile Thr Leu Asp Glu Ile Lys Glu Ser Thr Glu Asn Met
130 135 140
<210> 44
<211> 153
<212> PRT
<213> 向日葵（Helianthus annuus）
<220>
<221> MISC_FEATURE
<222> (1)..(153)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 44
Met Thr Leu Met Arg Thr Leu Pro Ala Thr Thr Ser Tyr Ala Val Gly
1 5 10 15
Phe Gly Val Ser Ser Phe Asn Ala Pro Pro Ser His Leu Arg Tyr Arg
20 25 30
Arg Phe Val Ile Ala Asn Ala Ser Ala Glu Pro Ser Asp Lys Ser Val
35 40 45
Glu Ile Met Arg Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr
50 55 60
Tyr Phe Cys Val Asp Lys Ser Val Thr Ser Val Val Ile Lys Gly Leu
65 70 75 80
Ala Asp His Lys Asp Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg His
85 90 95
Tyr Asp Asp Lys Ala Ala Glu Ala Thr Gln Gly Phe Trp Asn Cys Pro
100 105 110
Cys Val Pro Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu
115 120 125
Thr Pro Asp Asn Asp Phe Ala Gly Asn Glu Gln Val Ile Thr Met Glu
130 135 140
Glu Ile Lys Glu Ser Thr Ala Asn Met
145 150
<210> 45
<211> 144
<212> PRT
<213> 甜瓜（Cucumis melo）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 45
Met Ile Leu Gln Ala Ser Ser Phe Asn Ile Ala Val Pro Leu Ser Gly
1 5 10 15
Ser Ser Leu Gly Cys Ser Arg His Arg His Val Val Arg Ala Gln Ala
20 25 30
Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val Thr
50 55 60
Ala Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Thr Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Pro Ala Glu Val Ala
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Glu Asn Asp Phe Ala Gly Asn
115 120 125
Asp Gln Ala Ile Ser Leu Glu Asp Ile Arg Ala Thr Thr Ala Asn Met
130 135 140
<210> 46
<211> 148
<212> PRT
<213> 辣椒（Capsicum annuum）
<220>
<221> MISC_FEATURE
<222> (1)..(148)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 46
Met Gly Thr Leu Gln Ala Ser Thr Ser Tyr Ser Ile Gly Phe Gly Ile
1 5 10 15
Ser Ser Leu Ala Ala Leu Pro Lys Pro Ser Thr Arg Arg Cys Leu Thr
20 25 30
Val Ala Lys Met Glu Pro Ser Glu Lys Ser Val Glu Val Met Arg Lys
35 40 45
Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Met Asp
50 55 60
Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp
65 70 75 80
Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala
85 90 95
Ala Glu Ala Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp
115 120 125
Phe Ala Gly Glu Glu Gln Thr Ile Ser Met Glu Glu Ile Lys Glu Thr
130 135 140
Thr Ala Asn Met
145
<210> 47
<211> 145
<212> PRT
<213> 芝麻（Sesamum indicum）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 47
Met Arg Ala Leu Gln Ala Ser Thr Ser Tyr Ser Val Ser Phe Ala Ile
1 5 10 15
Pro Ser Val Ala Arg Pro Pro Pro Arg Arg His Gly Ile Val Ala Lys
20 25 30
Val Glu Pro Thr Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Thr Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala
85 90 95
Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Glu Gln Val Ile Ser Leu Glu Glu Ile Lys Glu Thr Thr Ala Asn
130 135 140
Met
145
<210> 48
<211> 148
<212> PRT
<213> 克莱门柚（Citrus clementina）
<220>
<221> MISC_FEATURE
<222> (1)..(148)
<400> 48
Met Thr Leu Gln Ser Ser Leu Cys Gly Ser Gly Val Ser Thr Phe Ile
1 5 10 15
Cys Thr Pro Arg Pro Ile Ile Ala Arg Ser Arg Pro Val Thr Gln Ile
20 25 30
Arg Ala Gln Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys
35 40 45
Phe Ser Glu Gln Tyr Ala Arg Arg Ser Asp Thr Phe Phe Cys Val Asp
50 55 60
Lys Ser Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp
65 70 75 80
Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala
85 90 95
Ala Glu Ala Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Glu Asn Asp
115 120 125
Phe Ala Gly Gln Asp Gln Ser Ile Ser Leu Asp Glu Ile Lys Glu Ser
130 135 140
Thr Ala Asn Met
145
<210> 49
<211> 144
<212> PRT
<213> 菜豆（Phaseolus vulgaris）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 49
Met Thr Thr Gln Ala Ser Ala Phe Ser Leu Gly Val Pro Ser Val Ala
1 5 10 15
Thr Pro Phe Arg Arg His Arg Asn Pro Val Thr Val Arg Ala Arg Ala
20 25 30
Glu Pro Ser Asp Lys Ser Val Asp Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Ala Asp Lys Gly Val Thr
50 55 60
Ala Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala Ala
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Asp
115 120 125
Glu Gln Ala Ile Thr Leu Asp Glu Ile Lys Glu Ser Thr Ala Asn Met
130 135 140
<210> 50
<211> 144
<212> PRT
<213> 芝麻（Sesamum indicum）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 50
Met Arg Ala Leu Gln Ala Ser Thr Ser Tyr Ser Val Ser Phe Ala Ile
1 5 10 15
Pro Ser Val Ala Arg Pro Pro Pro Arg Arg His Gly Ile Val Ala Lys
20 25 30
Glu Pro Thr Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val Thr
50 55 60
Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Thr Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala Gln
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Gln
115 120 125
Glu Gln Val Ile Ser Leu Glu Glu Ile Lys Glu Thr Thr Ala Asn Met
130 135 140
<210> 51
<211> 146
<212> PRT
<213> 琴叶拟南芥（Arabidopsis lyrata）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 51
Met Asn Leu Gln Ala Val Ser Cys Ser Phe Gly Phe Val Ser Ser Pro
1 5 10 15
Leu Gly Val Thr Pro Arg Thr Ser Phe Arg Arg Phe Val Ile Arg Ala
20 25 30
Lys Thr Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly
50 55 60
Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser Tyr
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu
85 90 95
Val Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Lys Asp Gln Thr Ile Thr Ser Asp Glu Ile Lys Glu Thr Thr Ala
130 135 140
Asn Met
145
<210> 52
<211> 146
<212> PRT
<213> 塔拟南芥（Arabidopsis thaliana）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 52
Met Asn Leu Gln Ala Val Ser Cys Ser Phe Gly Phe Leu Ser Ser Pro
1 5 10 15
Leu Gly Val Thr Pro Arg Thr Ser Phe Arg Arg Phe Val Ile Arg Ala
20 25 30
Lys Thr Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly
50 55 60
Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser Tyr
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu
85 90 95
Val Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Lys Asp Gln Thr Ile Thr Ser Asp Glu Ile Lys Glu Thr Thr Ala
130 135 140
Asn Met
145
<210> 53
<211> 146
<212> PRT
<213> 酸枣（Ziziphus jujuba）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 53
Met Ser Ile Gln Ala Thr Cys Ser Ser Phe Gly Val Gly Ile Ser Ser
1 5 10 15
Phe Val Ser Pro Leu Thr Arg Ser Arg His Arg Thr Leu Ile Arg Ala
20 25 30
Gln Val Gln Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly
50 55 60
Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Thr Leu
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Pro Ala Glu
85 90 95
Ala Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Glu Asn Asp Phe Ala
115 120 125
Gly Lys Glu Gln Thr Ile Ser Phe Asp Glu Ile Lys Glu Ala Thr Ala
130 135 140
Asn Met
145
<210> 54
<211> 148
<212> PRT
<213> 甜橙（Citrus sinensis）
<220>
<221> MISC_FEATURE
<222> (1)..(148)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 54
Met Thr Leu Gln Ser Ser Leu Cys Gly Ser Gly Val Ser Thr Phe Ile
1 5 10 15
Cys Thr Pro Arg Pro Ile Ile Ala Arg Pro Arg Pro Val Thr Gln Ile
20 25 30
Arg Ala Gln Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys
35 40 45
Phe Ser Glu Gln Tyr Ala Arg Arg Ser Asp Thr Phe Phe Cys Val Asp
50 55 60
Lys Ser Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp
65 70 75 80
Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala
85 90 95
Ala Glu Ala Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Glu Asn Asp
115 120 125
Phe Ala Gly Gln Asp Gln Ser Ile Ser Leu Asp Glu Ile Lys Glu Ser
130 135 140
Thr Ala Asn Leu
145
<210> 55
<211> 145
<212> PRT
<213> 葡萄（Vitis vinifera）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 55
Met Ser Leu Gln Ala Ser Ser Ser Tyr Ala Phe Gly Ile Ser Ser Phe
1 5 10 15
Val Ser Pro Pro Arg Arg Val Arg His Val Pro Glu Ile Arg Ala Lys
20 25 30
Val Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala
85 90 95
Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Asp Gln Thr Ile Ser Leu Glu Glu Ile Arg Glu Ser Thr Ala Asn
130 135 140
Met
145
<210> 56
<211> 146
<212> PRT
<213> 苹果（Malus domestica）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 56
Met Thr Leu Gln Leu Gln Ala Pro Ser Phe Ala Ala Gly Ile Ser Ser
1 5 10 15
Phe Val Ser Pro Leu Asn Arg Ser Thr Arg Arg Pro Val Ile Arg Ala
20 25 30
Lys Ala Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly
50 55 60
Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Pro Ala Glu
85 90 95
Ala Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Pro Glu Gln Thr Ile Ser Leu Glu Glu Ile Arg Glu Ser Thr Ala
130 135 140
Asn Met
145
<210> 57
<211> 146
<212> PRT
<213> 亚麻荠（Camelina sativa）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 57
Met Asn Leu Gln Ala Ala Ser Cys Ser Phe Gly Phe Val Ser Ser Pro
1 5 10 15
Leu Gly Val Thr Pro Gly Thr Ser Phe Arg Arg Phe Val Ile Arg Ala
20 25 30
Lys Ser Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly
50 55 60
Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser Tyr
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu
85 90 95
Val Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Lys Asp Gln Thr Ile Thr Ser Glu Glu Ile Lys Glu Thr Thr Ala
130 135 140
Asn Met
145
<210> 58
<211> 150
<212> PRT
<213> 芦笋（Asparagus officinalis）
<220>
<221> MISC_FEATURE
<222> (1)..(150)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 58
Met Ser Ile Gln Ala Gln Ala Ser Ala Gly Val Gly Ala Phe Val Gly
1 5 10 15
Val Gly Pro Arg Leu Pro Pro Pro Pro Ser Ile Arg Arg Ser Arg Arg
20 25 30
Val Val Arg Ala Lys Ala Glu Pro Ser Glu Lys Ser Val Glu Ile Met
35 40 45
Arg Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys
50 55 60
Val Asp Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His
65 70 75 80
Lys Asp Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp
85 90 95
Lys Ala Ala Glu Ala Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro
100 105 110
Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp
115 120 125
Asn Asp Phe Ala Gly Ala Asp Gln Thr Ile Thr Leu Glu Glu Ile Lys
130 135 140
Glu Ser Thr Ser Ser Leu
145 150
<210> 59
<211> 148
<212> PRT
<213> 脐橙（Citrus sinensis）
<220>
<221> MISC_FEATURE
<222> (1)..(148)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 59
Met Thr Leu Gln Ser Ser Leu Cys Gly Ser Gly Val Ser Thr Phe Ile
1 5 10 15
Cys Thr Pro Arg Pro Ile Ile Ala Arg Pro Arg Pro Val Thr Gln Ile
20 25 30
Arg Ala Gln Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys
35 40 45
Phe Ser Glu Gln Tyr Ala Arg Arg Ser Asp Thr Phe Phe Cys Val Asp
50 55 60
Lys Ser Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp
65 70 75 80
Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala
85 90 95
Ala Glu Ala Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Glu Asn Asp
115 120 125
Phe Ala Gly Gln Asp Gln Ser Ile Ser Leu Asp Glu Ile Lys Glu Ser
130 135 140
Thr Ala Asn Met
145
<210> 60
<211> 144
<212> PRT
<213> 蒺藜苜蓿（Medicago truncatula）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 60
Met Thr Ser Gln Ala Ser Thr Phe Ala Val Ser Val Ser Ser Ala Pro
1 5 10 15
Ser Pro Leu Arg Arg Arg Arg Thr Phe Pro Val Val Arg Ala Gln Leu
20 25 30
Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Lys Ser Gly Thr Phe Phe Cys Ser Asp Lys Gly Val Thr
50 55 60
Ala Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala Ala
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Asp
115 120 125
Glu Gln Thr Ile Thr Leu Asp Glu Ile Lys Glu Ser Thr Ala Asn Met
130 135 140
<210> 61
<211> 146
<212> PRT
<213> 荠菜（Capsella rubella）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 61
Met Asn Leu Gln Ala Val Ser Cys Ser Phe Gly Phe Val Ser Ser Pro
1 5 10 15
Leu Gly Val Thr Pro Arg Thr Ser Phe Arg Arg Phe Val Ile Arg Ala
20 25 30
Lys Thr Glu Pro Ser Glu Lys Ser Ile Glu Ile Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly
50 55 60
Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser Tyr
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu
85 90 95
Val Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Lys Asp Gln Thr Ile Thr Ser Asp Glu Ile Lys Glu Thr Thr Ala
130 135 140
Asn Met
145
<210> 62
<211> 151
<212> PRT
<213> 胡萝卜（Daucus carota）
<220>
<221> MISC_FEATURE
<222> (1)..(151)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 62
Met Arg Ser Leu Gln Ala Ser Thr Ser Tyr Thr Thr Ser Leu Ser Ser
1 5 10 15
Ala Val Pro Ser Val Ala Asn Tyr Ser Pro Arg Thr Leu His His Arg
20 25 30
His Val Ile Ser Ala Lys Gly Glu Pro Thr Glu Lys Ser Val Glu Ile
35 40 45
Met Arg Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe
50 55 60
Cys Val Asp Lys Ser Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp
65 70 75 80
His Lys Asp Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp
85 90 95
Asp Lys Ala Ala Glu Ala Val Gln Gly Phe Trp Asn Cys Pro Cys Val
100 105 110
Pro Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Thr
115 120 125
Asp Asn Asp Phe Ala Gly Gln Asp Gln Ala Ile Ser Leu Glu Glu Ile
130 135 140
Lys Glu Thr Thr Ala Gly Met
145 150
<210> 63
<211> 144
<212> PRT
<213> 大豆（Glycine max）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 63
Met Thr Thr Gln Ala Ser Thr Phe Ala Val Ala Val Pro Ser Val Thr
1 5 10 15
Thr Pro Phe Arg Ser His Arg Asn Pro Phe Val Val Arg Ala Gln Ala
20 25 30
Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val Thr
50 55 60
Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Thr Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val Ala
85 90 95
Gln Gly Phe Arg Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Asn
115 120 125
Glu Gln Thr Ile Thr Leu Asp Glu Ile Lys Glu Ser Thr Ala Asn Met
130 135 140
<210> 64
<211> 148
<212> PRT
<213> 马铃薯（Solanum tuberosum）
<220>
<221> MISC_FEATURE
<222> (1)..(148)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 64
Met Arg Thr Leu Gln Ala Ser Thr Ser Tyr Ser Val Gly Phe Gly Ile
1 5 10 15
Ser Ser Phe Ala Thr Arg Pro Lys Pro Ser Thr His Arg Cys Leu Thr
20 25 30
Val Ala Lys Met Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys
35 40 45
Phe Ser Glu Gln Tyr Ala Arg Arg Ser Glu Thr Tyr Phe Cys Met Asp
50 55 60
Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp
65 70 75 80
Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala
85 90 95
Ala Glu Ala Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp
115 120 125
Phe Ala Gly Glu Glu Gln Thr Ile Ser Met Glu Glu Ile Lys Glu Thr
130 135 140
Thr Ala Asn Met
145
<210> 65
<211> 150
<212> PRT
<213> 芝麻（Sesamum indicum）
<220>
<221> MISC_FEATURE
<222> (1)..(150)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 65
Met Arg Ala Leu Gln Ala Ser Thr Ser Tyr Ser Val Ala Phe Ala Ile
1 5 10 15
Ser Ser Val Ala Pro Leu Pro Ser Leu Pro Arg Pro Arg Gln Arg His
20 25 30
Val Ile Ser Ala Asn His Glu Pro Thr Glu Lys Ser Val Glu Ile Met
35 40 45
Arg Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys
50 55 60
Val Asp Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His
65 70 75 80
Lys Asp Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp
85 90 95
Lys Ala Ala Glu Ala Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro
100 105 110
Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp
115 120 125
Asn Asp Phe Ala Gly Gln Glu Gln Asn Ile Ser Leu Glu Glu Ile Lys
130 135 140
Glu Thr Thr Ala Asn Leu
145 150
<210> 66
<211> 144
<212> PRT
<213> 木豆（Cajanus cajan）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 66
Met Thr Ala Gln Ala Ser Ser Phe Ser Val Ala Val Ser Ser Val Ala
1 5 10 15
Thr Pro Phe Arg Arg His Arg Asn Pro Phe Val Val Arg Ala Gln Ala
20 25 30
Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val Thr
50 55 60
Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val Ala
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Asp
115 120 125
Glu Gln Thr Ile Thr Leu Asp Glu Ile Lys Glu Ser Thr Ala Asn Met
130 135 140
<210> 67
<211> 148
<212> PRT
<213> 胡桃（Juglans regia）
<220>
<221> MISC_FEATURE
<222> (1)..(148)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 67
Met Thr Leu Gln Ala Gln Ala Ser Ser Ser Ser Phe Ala Val Gly Ile
1 5 10 15
Pro Ser Phe Thr Ser Pro Leu Gly Gly Ser Arg His Arg Leu Val Val
20 25 30
Arg Ala Lys Val Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys
35 40 45
Phe Ser Glu Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp
50 55 60
Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp
65 70 75 80
Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Pro
85 90 95
Ala Glu Ala Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp
115 120 125
Phe Ala Gly Lys Glu Gln Thr Ile Ser Leu Glu Glu Ile Arg Glu Ser
130 135 140
Thr Ala Asn Met
145
<210> 68
<211> 146
<212> PRT
<213> 梨属x白梨（Pyrus x bretschneideri）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 68
Met Thr Leu Gln Leu Gln Ala Pro Ser Phe Ala Ala Ser Ile Ser Ser
1 5 10 15
Phe Val Ser Pro Leu Asn Arg Pro Thr Arg Arg Pro Val Ile Arg Ala
20 25 30
Lys Ala Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly
50 55 60
Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Pro Ala Glu
85 90 95
Ala Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Pro Glu Gln Ala Ile Ser Leu Glu Glu Ile Arg Glu Ser Thr Ala
130 135 140
Asn Met
145
<210> 69
<211> 148
<212> PRT
<213> 油棕（Elaeis guineensis）
<220>
<221> MISC_FEATURE
<222> (1)..(148)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 69
Met Ala Ala Met Ser Thr Arg Thr Ala Phe His Gly Val Pro Leu Pro
1 5 10 15
Ile Phe Gln Pro Ala Ser Pro Pro Ser Arg Pro Tyr Arg Leu Leu Val
20 25 30
Arg Ser Lys Val Glu Pro Ser Glu Lys Ser Val Asp Ile Met Arg Arg
35 40 45
Phe Ser Glu Lys Tyr Ala Gln Gln Ser Gly Thr Tyr Phe Cys Ala Asp
50 55 60
Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp
65 70 75 80
Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala
85 90 95
Ala Glu Val Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp
115 120 125
Phe Ala Gly Thr Glu Gln Thr Ile Thr Leu Asp Glu Ile Lys Glu Ala
130 135 140
Thr Ser Lys Val
145
<210> 70
<211> 149
<212> PRT
<213> 黄猴花（Erythranthe guttata）
<220>
<221> MISC_FEATURE
<222> (1)..(149)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 70
Met Thr Ala Leu Gln Ala Ser Thr Ser Tyr Ser Val Gly Phe Ala Ile
1 5 10 15
Ser Ser Ala Ala Pro Pro Pro Leu Pro Ser Arg His Leu Arg His Gly
20 25 30
Val Val Ala Lys Val Glu Pro Thr Glu Lys Ser Val Glu Val Met Arg
35 40 45
Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val
50 55 60
Asp Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys
65 70 75 80
Asp Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys
85 90 95
Ala Ala Glu Ala Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met
100 105 110
Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn
115 120 125
Asp Phe Ala Gly Gln Glu Gln Val Ile Ser Met Glu Glu Ile Arg Glu
130 135 140
Thr Thr Ala Asn Met
145
<210> 71
<211> 150
<212> PRT
<213> 毛果杨x美洲黑杨（Populus trichocarpa x Populus deltoides）
<220>
<221> MISC_FEATURE
<222> (1)..(150)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 71
Met Asn Trp Ser Cys Ser Thr Ala Thr Thr Cys Gly Ile Ser Ser Ser
1 5 10 15
Ser Ser Cys Phe Ser Cys Pro Pro Lys Thr Pro Ser Phe Arg Ser Val
20 25 30
Ile Arg Ala Ser Lys Val Glu Pro Ser Asp Lys Ser Val Glu Ile Met
35 40 45
Arg Lys Phe Ser Glu Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys
50 55 60
Val Asp Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His
65 70 75 80
Lys Asp Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp
85 90 95
Lys Ala Ala Glu Ala Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro
100 105 110
Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp
115 120 125
Asn Asp Phe Ala Gly Lys Asp Gln Thr Ile Ser Leu Glu Glu Ile Arg
130 135 140
Glu Thr Thr Ala Asn Met
145 150
<210> 72
<211> 145
<212> PRT
<213> 红豆（Vigna angularis）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 72
Met Thr Thr Gln Ala Ser Ala Phe Ser Leu Ala Ala Pro Ser Val Ala
1 5 10 15
Thr Pro Phe Arg Arg Arg His Arg Asn Pro Leu Leu Val Arg Ala Ala
20 25 30
Val Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala
85 90 95
Ala Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Asp Glu Gln Ala Ile Thr Leu Glu Glu Ile Lys Glu Ser Thr Ala Asn
130 135 140
Met
145
<210> 73
<211> 144
<212> PRT
<213> 黄瓜（Cucumis sativus）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 73
Met Ile Leu Gln Ala Ser Ser Phe Asn Ile Ala Val Pro Leu Tyr Gly
1 5 10 15
Ser Ser Leu Gly Cys Ser Arg His Arg His Val Val Arg Ala Gln Ala
20 25 30
Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val Thr
50 55 60
Ala Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Thr Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Pro Ala Glu Val Ala
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Glu Asn Asp Phe Ala Gly Asn
115 120 125
Asp Gln Ala Ile Ser Leu Glu Asp Ile Arg Ala Thr Thr Ala Asn Met
130 135 140
<210> 74
<211> 145
<212> PRT
<213> 雷蒙德氏棉（Gossypium raimondii）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 74
Met Thr Leu Gln Ala Thr Ser Cys Asn Phe Gly Ala Ile Ser Ser Leu
1 5 10 15
Leu Arg Pro Pro Lys Ile Ser Arg His Arg Phe Val Ile Arg Ala Glu
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Met Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val
85 90 95
Ser Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Asp Gln Thr Ile Thr Leu Glu Glu Ile Lys Glu Thr Thr Ala Asn
130 135 140
Met
145
<210> 75
<211> 145
<212> PRT
<213> 荷花（Nelumbo nucifera）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 75
Met Thr Val Gln Ala Ser Phe Tyr Ser Val Gly Val Pro Ser Phe Ala
1 5 10 15
Ala Pro Pro Pro Asn Arg Ser Arg His Val Ile Arg Ala Lys Ala Lys
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala
85 90 95
Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Lys Glu Gln Ala Ile Ser Leu Glu Glu Ile Arg Glu Ala Thr Ala Asn
130 135 140
Met
145
<210> 76
<211> 144
<212> PRT
<213> 菠菜（Spinacia oleracea）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 76
Met Lys Ala Leu Gln Ala Ser Ile Ala Tyr Ser Phe Pro Ile Ser Ser
1 5 10 15
Pro Ala Ala Ser Pro Arg Arg Phe Ser Arg Val Ile Arg Ala Gln Ala
20 25 30
Asp Pro Ser Asp Lys Ser Met Glu Val Met Arg Lys Phe Ser Glu Gln
35 40 45
Phe Cys Arg Lys Ser Asp Thr Tyr Phe Cys Val Asp Lys Ser Val Thr
50 55 60
Ala Val Val Ile Lys Gly Leu Ala Asp His Arg Asp Thr Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Glu Ala Glu Ala Lys
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Lys
115 120 125
Glu Gln Thr Ile Thr Leu Asp Glu Ile Arg Glu Val Thr Ser Asn Met
130 135 140
<210> 77
<211> 146
<212> PRT
<213> 苹果（Malus domestica）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<220>
<221> MISC_FEATURE
<222> (8)..(8)
<223> Xaa指代任何氨基酸
<400> 77
Met Thr Leu Gln Leu Gln Ala Xaa Ser Phe Gly Ala Ser Ile Ala Ser
1 5 10 15
Phe Val Ser Pro Leu Asn Arg Ser Thr Arg Arg Pro Val Ile Ile Ala
20 25 30
Asn Ala Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly
50 55 60
Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Pro Ala Glu
85 90 95
Ala Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Pro Glu Gln Ala Ile Ser Leu Glu Glu Ile Lys Glu Ser Thr Ala
130 135 140
Asn Met
145
<210> 78
<211> 145
<212> PRT
<213> 树棉（Gossypium arboreum）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 78
Met Thr Leu Gln Ala Ser Phe Phe Asn Phe Gly Ser Ile Phe Ser Leu
1 5 10 15
Pro Cys Pro Pro Arg Thr Ser Arg Gln Arg Phe Val Ile Arg Ala Lys
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Met Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Thr Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val
85 90 95
Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Asp Gln Ser Ile Thr Leu Glu Glu Ile Lys Glu Thr Thr Thr Asn
130 135 140
Met
145
<210> 79
<211> 149
<212> PRT
<213> 番茄（Solanum lycopersicum）
<220>
<221> MISC_FEATURE
<222> (1)..(149)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 79
Met Arg Thr Leu Gln Ala Ser Thr Ser Tyr Ser Val Gly Phe Gly Ile
1 5 10 15
Ser Ser Phe Ala Thr Arg Pro Lys Pro Ser Ser Thr His Arg Cys Leu
20 25 30
Thr Val Ala Lys Met Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg
35 40 45
Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Glu Thr Tyr Phe Cys Met
50 55 60
Asp Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys
65 70 75 80
Asp Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys
85 90 95
Ala Ala Glu Ala Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met
100 105 110
Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn
115 120 125
Asp Phe Ala Gly Glu Glu Gln Thr Ile Thr Met Glu Glu Ile Lys Glu
130 135 140
Thr Thr Ala Asn Met
145
<210> 80
<211> 145
<212> PRT
<213> 川桑（Morus notabilis）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 80
Met Thr Val Gln Ala Ser Thr Ser Phe Ser Phe Gly Ile Ser Ser Phe
1 5 10 15
Val Ile Pro Pro Ala Arg Ser Arg His Arg Ser Val Ile Arg Ala Gln
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Gln Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala
85 90 95
Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Glu Gln Thr Val Thr Leu Glu Glu Ile Lys Glu Ser Thr Ala Asn
130 135 140
Met
145
<210> 81
<211> 175
<212> PRT
<213> 乌拉尔图小麦（Triticum urartu）
<220>
<221> MISC_FEATURE
<222> (1)..(175)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 81
Met Ser Ile Thr Ser Thr Ala Ala Ala Ala Ala Gly Ser Pro Val Tyr
1 5 10 15
Thr Pro Leu Val Pro Arg Gly Gly Arg Arg Ser Arg Cys Ala Val Arg
20 25 30
Ala Gln Ala Ala Gly Glu Gly Ala Ala Ala Ala Asp Gly Ala Ala Ser
35 40 45
Glu His Lys Ser Leu Glu Ile Met Arg Lys Phe Ser Glu Gln Tyr Ala
50 55 60
Arg Arg Ser Ser Thr Phe Phe Cys Ser Asp Lys Ser Val Thr Ala Val
65 70 75 80
Val Ile Lys Gly Leu Ala Asp His Lys Glu Gln Leu Gly Ala Pro Leu
85 90 95
Cys Pro Cys Arg Gln Val Gly Gln Ser Tyr Arg His Tyr Asp Asp Lys
100 105 110
Ala Ala Glu Ala Ala Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met
115 120 125
Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn
130 135 140
Asp Phe Ala Gly Glu Asp Gln Ala Arg Leu Arg Phe Leu Ser Val Leu
145 150 155 160
Ser Ala Ile Ser Leu Glu Glu Ile Lys Glu Ala Thr Ser Lys Tyr
165 170 175
<210> 82
<211> 148
<212> PRT
<213> 茸毛烟草（Nicotiana tomentosiformis）
<220>
<221> MISC_FEATURE
<222> (1)..(148)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 82
Met Arg Thr Leu Gln Ala Ser Thr Ser Tyr Ser Val Gly Phe Gly Ile
1 5 10 15
Ser Ser Phe Ala Thr Leu Pro Lys Pro Ser Thr Arg Arg Asp Val Ala
20 25 30
Val Ala Lys Met Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys
35 40 45
Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Met Asp
50 55 60
Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp
65 70 75 80
Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala
85 90 95
Ala Glu Ala Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp
115 120 125
Phe Ala Gly Glu Glu Gln Ala Ile Ser Met Glu Glu Ile Lys Glu Thr
130 135 140
Thr Ala Asn Met
145
<210> 83
<211> 210
<212> PRT
<213> 大叶藻（Zostera marina）
<220>
<221> MISC_FEATURE
<222> (1)..(210)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 83
Met Asp Gly Pro Arg Leu Leu Leu Pro Asn Ala Val Pro Ile Phe Leu
1 5 10 15
Ala Lys Leu Arg Pro His Arg Leu Ile Glu Pro Gln Asn Gln Lys Arg
20 25 30
Glu Arg Arg Thr Lys Arg Ile Leu Glu Gln Gly Cys Leu Ser Val Ala
35 40 45
Met Ser Ile Val Asn Arg Thr Ser Phe Tyr Gly Val Ala Ile Pro Ser
50 55 60
Ser Thr Leu Thr Ser Thr Val Arg Ala Arg Pro Phe Ser Thr Ile His
65 70 75 80
Tyr Pro Pro Arg His Gly Leu Pro Thr Gly Ile Arg Ala Glu Gln Ser
85 90 95
Ala Val Asp Pro Ala Asp Lys Ser Val Glu Thr Met Arg Lys Phe Ser
100 105 110
Glu Gln Tyr Ala Arg Arg Ser Asp Thr Phe Phe Cys Val Asp Lys Ser
115 120 125
Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Thr Ser Leu
130 135 140
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu
145 150 155 160
Ala Lys Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
165 170 175
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
180 185 190
Gly Thr Asp Gln Thr Ile Thr Met Glu Glu Ile Ser Glu Thr Val Asn
195 200 205
Gly Ala
210
<210> 84
<211> 144
<212> PRT
<213> 碱蓬（Suaeda glauca）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 84
Met Lys Ser Leu Gln Ala Ser Thr Ala Tyr Ser Phe Pro Ile Ser Ser
1 5 10 15
Pro Ser Ile Pro Pro Lys Arg Phe Ser Arg Val Ile Arg Ala Gln Val
20 25 30
Glu Pro Ser Asp Lys Ser Met Glu Val Met Arg Lys Phe Ser Glu Gln
35 40 45
Phe Cys Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Ser Val Thr
50 55 60
Ala Val Val Ile Lys Gly Leu Ala Asp His Arg Asp Thr Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala Ser
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Lys
115 120 125
Asp Gln Thr Ile Thr Leu Asp Glu Ile Arg Glu Val Thr Ala Asn Met
130 135 140
<210> 85
<211> 145
<212> PRT
<213> 陆地棉（Gossypium hirsutum）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 85
Met Thr Leu Gln Ala Thr Ser Cys Asn Phe Gly Ala Ile Ser Ser Leu
1 5 10 15
Leu Arg Pro Pro Lys Ile Ser Arg His Arg Phe Val Ile Arg Ala Glu
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Met Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val
85 90 95
Asn Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Asp Gln Thr Ile Thr Leu Glu Glu Ile Lys Glu Thr Thr Ala Asn
130 135 140
Met
145
<210> 86
<211> 148
<212> PRT
<213> 烟草（Nicotiana attenuata）
<220>
<221> MISC_FEATURE
<222> (1)..(148)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 86
Met Arg Thr Leu Gln Ala Ser Thr Ser Tyr Ser Val Gly Phe Gly Ile
1 5 10 15
Ser Ser Phe Ala Thr Leu Pro Lys Pro Ser Thr Arg Arg Asp Val Thr
20 25 30
Val Ala Lys Met Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys
35 40 45
Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Met Asp
50 55 60
Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp
65 70 75 80
Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala
85 90 95
Ala Glu Ala Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp
115 120 125
Phe Ala Gly Glu Glu Gln Ala Ile Ser Met Glu Glu Ile Lys Glu Thr
130 135 140
Thr Ala Asn Met
145
<210> 87
<211> 146
<212> PRT
<213> 野草莓（Fragaria vesca）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 87
Met Thr Leu Gln Leu Gln Ala Pro Ser Phe Ala Ala Thr Ile Ser Ser
1 5 10 15
Leu Ala Ser Pro Leu Asn Arg Ser Thr Arg Arg Phe Val Ile Arg Ala
20 25 30
Gln Val Glu Pro Thr Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly
50 55 60
Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Pro Ala Glu
85 90 95
Ala Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Glu Asn Asp Phe Ala
115 120 125
Gly Lys Glu Gln Thr Ile Thr Leu Glu Glu Ile Arg Glu Ser Thr Ala
130 135 140
Asn Met
145
<210> 88
<211> 148
<212> PRT
<213> 黄猴花（Erythranthe guttata）
<220>
<221> MISC_FEATURE
<222> (1)..(148)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 88
Met Arg Ala Leu Gln Ala Ser Thr Ser Tyr Ser Ile Ser Ser Phe Gly
1 5 10 15
Ile Ser Ser Ala Ala Pro Ser Pro Pro Ser Arg Arg Arg His Val Val
20 25 30
Phe Ser Lys Ala Glu Pro Thr Glu Lys Ser Val Glu Ile Met Arg Lys
35 40 45
Phe Ser Glu Gln Tyr Ala Arg Arg Ser Asp Thr Tyr Phe Cys Val Asp
50 55 60
Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp
65 70 75 80
Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala
85 90 95
Ala Glu Ala Gln Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg
100 105 110
Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Glu Asn Asp
115 120 125
Phe Ala Gly Gln Asp Gln Thr Ile Thr Leu Glu Glu Ile Arg Glu Thr
130 135 140
Thr Ser Asn Met
145
<210> 89
<211> 391
<212> PRT
<213> 石榴（Punica granatum）
<220>
<221> MISC_FEATURE
<222> (1)..(391)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 89
Met Lys Pro Val Ser Phe Leu Leu Cys Leu Ile Phe Leu Val Val Gly
1 5 10 15
Phe Arg Glu Ser Val Gln Gly Arg Pro Leu Ser Val Ser Lys Pro Asp
20 25 30
Pro Arg Asn Ala Ala Ala Thr Ala Arg Trp Leu Val Ala Gln Asn Ser
35 40 45
Trp Gly Val Leu Asn Thr Ile Ser Ser Glu Leu Gly Gly Ala Pro Phe
50 55 60
Gly Asn Val Val Ser Phe Ser Asp Gly Ser Pro Asn Glu Gly Arg Gly
65 70 75 80
Ile Pro Tyr Phe Tyr Leu Thr Ala Leu Asp Pro Thr Ala Lys Asn Ala
85 90 95
Leu Lys Asp Gly Arg Ala Ser Leu Thr Val Ser Glu Tyr Pro Leu Gly
100 105 110
Thr Cys Gly Lys Ile Asp Pro Glu Asn Pro Thr Cys Ala Lys Leu Thr
115 120 125
Leu Thr Gly Lys Leu Lys Ile Val Glu Glu Lys Ser Asp Glu Ala Gln
130 135 140
Tyr Ala Lys Ser Ala Leu Phe Thr Lys His Pro Glu Met Lys Thr Trp
145 150 155 160
Pro Lys Ser His Lys Phe Gln Phe Phe Lys Leu Glu Ile Glu Asn Ile
165 170 175
Phe Leu Ile Asp Trp Phe Gly Gly Pro Lys Pro Leu Thr Pro Glu Gln
180 185 190
Tyr Leu His Pro Lys Thr Ile Glu Ser Gln Ser Ser Phe Leu Leu Leu
195 200 205
Glu Ala Val Glu Ser Glu Val Ser Ser Lys Thr Leu Gln Val Trp Ser
210 215 220
Trp Lys Leu Ser Thr Ala Thr Leu Leu Ile Val Trp Lys Leu Arg Leu
225 230 235 240
Gln Ser Ala Gln Glu Thr Met Thr Leu Gln Ala Ser Ser Phe Ala Val
245 250 255
Gly Met Pro Ala Leu Ala Pro Pro Arg Leu Gly Arg Ser Arg His Ser
260 265 270
Cys Val Val Arg Ala Lys Val Glu Pro Leu Asp Lys Ser Val Glu Ile
275 280 285
Met Arg Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe
290 295 300
Cys Val Asp Lys Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp
305 310 315 320
His Lys Asp Ser Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp
325 330 335
Asp Lys Ala Ala Glu Ala Ala Gln Gly Phe Trp Asn Cys Pro Cys Val
340 345 350
Pro Met Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro
355 360 365
Glu Asn Asp Phe Ala Gly Thr Asp Gln Ser Ile Ser Leu Glu Glu Ile
370 375 380
Arg Glu Ser Thr Ala Asn Met
385 390
<210> 90
<211> 146
<212> PRT
<213> 白花羽扇豆（Lupinus angustifolius）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 90
Met Met Thr Leu Gln Ala Ser Thr Phe Ala Val Ala Ile Pro Ser Ser
1 5 10 15
Ala Val Thr His Leu Arg Arg His Arg Asn Phe Ser Ala Ile Arg Ala
20 25 30
Gln Val Glu Pro Ser Asp Lys Asn Val Glu Ile Met Arg Lys Phe Ser
35 40 45
Glu Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly
50 55 60
Val Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu
85 90 95
Ala Ser Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Gln Glu Gln Thr Ile Thr Leu Asp Glu Ile Lys Glu Ser Ile Val
130 135 140
Asn Met
145
<210> 91
<211> 145
<212> PRT
<213> 雷蒙德氏棉（Gossypium raimondii）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 91
Met Thr Leu Gln Ala Ser Phe Phe Asn Phe Gly Ser Ile Ser Ser Leu
1 5 10 15
Pro Cys Pro Pro Arg Thr Ser Arg Gln Arg Phe Val Ile Arg Ala Lys
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Thr Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala
85 90 95
Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Asp Gln Ala Ile Thr Leu Glu Glu Ile Lys Glu Thr Thr Ala Asn
130 135 140
Met
145
<210> 92
<211> 145
<212> PRT
<213> 可可树/豆（Theobroma cacao）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 92
Met Thr Leu Gln Ala Thr Ser Cys Asn Phe Gly Gly Ile Ser Ser Leu
1 5 10 15
Leu Cys Pro Pro Lys Thr Ser Leu His Arg Phe Val Ile Arg Ala Lys
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Met Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Thr Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val
85 90 95
Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Asp Gln Ser Ile Thr Ser Glu Glu Ile Lys Glu Thr Thr Ala Asn
130 135 140
Met
145
<210> 93
<211> 145
<212> PRT
<213> 盐水芹（Eutrema salsugineum）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 93
Met Asn Leu Gln Ala Val Ser Cys Ser Phe Gly Phe Val Ser Val Pro
1 5 10 15
Leu Val Ser Pro Arg Thr Ser Phe Arg Arg Phe Val Ile Arg Ala Lys
20 25 30
Ser Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser His Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val
85 90 95
Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Lys Asp Gln Thr Ile Thr Ser Glu Glu Ile Lys Glu Thr Thr Ala Asn
130 135 140
Met
145
<210> 94
<211> 145
<212> PRT
<213> 哥伦比亚锦葵（Herrania umbratica）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 94
Met Thr Leu Gln Ala Thr Ser Cys Asn Phe Gly Gly Ile Ser Ser Leu
1 5 10 15
Leu Cys Pro Pro Lys Thr Ser Gln His Arg Phe Val Ile Arg Ala Lys
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Met Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Thr Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Thr Ala Glu Val
85 90 95
Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Asp Gln Ser Ile Thr Leu Glu Glu Ile Lys Glu Thr Thr Ala Asn
130 135 140
Met
145
<210> 95
<211> 145
<212> PRT
<213> 陆地棉（Gossypium hirsutum）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 95
Met Thr Leu Gln Ala Thr Ser Cys Asn Phe Gly Ala Ile Ser Ser Leu
1 5 10 15
Leu Arg Pro Pro Lys Ile Ser Arg His Arg Phe Val Ile Arg Ala Glu
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Met Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala
85 90 95
Ser Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Asp Gln Thr Ile Thr Leu Glu Glu Ile Lys Glu Thr Thr Ala Asn
130 135 140
Met
145
<210> 96
<211> 144
<212> PRT
<213> 萝卜（Raphanus sativus）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 96
Met Asn Pro Gln Ala Val Ser Cys Ser Phe Gly Phe Val Ser Ala Pro
1 5 10 15
Leu Val Ser Pro Arg Arg Thr Ser Arg Phe Val Ile His Ala Lys Ser
20 25 30
Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val Thr
50 55 60
Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser Tyr Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val Gly
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Lys
115 120 125
Asp Gln Thr Ile Thr Ser Glu Glu Ile Lys Glu Thr Thr Ala His Met
130 135 140
<210> 97
<211> 146
<212> PRT
<213> 甜菜（Beta vulgaris）
<220>
<221> MISC_FEATURE
<222> (1)..(146)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 97
Met Thr Met Lys Ser Leu Gln Ala Ser Thr Ala Tyr Ser Phe Pro Ile
1 5 10 15
Ser Ser Pro Ser Ile Ser Pro Arg Arg Phe Ser Arg Val Ile Arg Ala
20 25 30
Gln Val Asp Pro Ser Glu Lys Ser Val Glu Val Met Arg Lys Phe Ser
35 40 45
Glu Gln Phe Cys Arg Lys Ser Glu Thr Tyr Phe Cys Ala Asp Lys Ser
50 55 60
Val Thr Ala Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Thr Leu
65 70 75 80
Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu
85 90 95
Ala Lys Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg
100 105 110
Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala
115 120 125
Gly Lys Asp Gln Thr Ile Ser Leu Asp Glu Ile Arg Glu Thr Thr Ala
130 135 140
Asn Met
145
<210> 98
<211> 144
<212> PRT
<213> 萝卜（Raphanus sativus）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 98
Met Asn Pro Gln Ala Val Ser Cys Ser Ser Phe Gly Phe Val Ser Ala
1 5 10 15
Pro Leu Val Ser Pro Arg Thr Ser Arg Phe Val Ile His Ala Lys Ser
20 25 30
Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val Thr
50 55 60
Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser Tyr Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val Gly
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Lys
115 120 125
Asp Gln Thr Ile Thr Ser Glu Glu Ile Lys Glu Thr Thr Ala His Met
130 135 140
<210> 99
<211> 147
<212> PRT
<213> 盐水芹（Eutrema salsugineum）
<220>
<221> MISC_FEATURE
<222> (1)..(147)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 99
Met Asn Leu Gln Ala Val Ser Cys Ser Phe Gly Cys Ala Ser Cys Val
1 5 10 15
Ser Leu His Ser Tyr Lys Ala Ser Phe Arg Arg Ser Phe Thr Ile Arg
20 25 30
Ala Lys Ser Glu Pro Ser Glu Lys Ser Ile Glu Ile Met Arg Lys Phe
35 40 45
Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys
50 55 60
Gly Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser
65 70 75 80
Tyr Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala
85 90 95
Glu Val Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu
100 105 110
Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe
115 120 125
Ala Gly Lys Asp Gln Thr Ile Thr Ser Glu Glu Ile Lys Glu Thr Thr
130 135 140
Ala Asn Met
145
<210> 100
<211> 144
<212> PRT
<213> 桃红蝴蝶兰（Phalaenopsis equestris）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 100
Met Ser Ile Gln Ser Ser Phe Ala Gly Ala Ala Leu Pro Leu Pro Val
1 5 10 15
Ala Val Pro Pro Gln Arg Arg Cys Leu Gly Leu Ile Arg Ala Lys Val
20 25 30
Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Arg Ser Glu Thr Tyr Phe Cys Val Asp Lys Gly Val Thr
50 55 60
Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Glu Thr Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala Ala
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Arg
115 120 125
Glu Gln Thr Ile Thr Leu Asp Glu Ile Lys Leu Ser Thr Ser Asn Leu
130 135 140
<210> 101
<211> 149
<212> PRT
<213> 北美云杉（Picea sitchensis）
<220>
<221> MISC_FEATURE
<222> (1)..(149)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 101
Met Ala Asn Ile Ala Ile Gly Leu Pro Asn Phe Ser Met Gly Cys Val
1 5 10 15
Tyr Lys Ser Gln His Phe Lys Asn Pro Ser Lys Arg Leu Ile Thr Val
20 25 30
Arg Ala Asn Lys Val Glu Pro Ser Asp Lys Ser Val Glu Ile Met Arg
35 40 45
Lys Phe Ser Glu Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val
50 55 60
Asp Lys Ala Val Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys
65 70 75 80
Asp Thr Leu Gly Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys
85 90 95
Val Thr Glu Ala Lys Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met
100 105 110
Arg Glu Arg Lys Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn
115 120 125
Asp Phe Ala Gly Lys Asp Gln Gly Ile Ser Ser Glu Glu Met Lys Glu
130 135 140
Leu Thr Val Asn Phe
145
<210> 102
<211> 145
<212> PRT
<213> 树棉（Gossypium arboreum）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 102
Met Thr Leu Gln Ala Thr Ser Cys Asn Phe Gly Ala Ile Ser Ser Leu
1 5 10 15
Leu Arg Pro Pro Lys Ile Ser Arg His Arg Phe Val Ile Arg Ala Glu
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Met Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asn Ser Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala
85 90 95
Ser Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Asp Gln Thr Ile Thr Leu Glu Glu Ile Lys Glu Thr Thr Ala Asn
130 135 140
Met
145
<210> 103
<211> 144
<212> PRT
<213> 铁皮石斛（Dendrobium catenatum）
<220>
<221> MISC_FEATURE
<222> (1)..(144)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 103
Met Ser Ile Gln Ser Ser Phe Ala Gly Val Ala Leu Pro Leu Pro Val
1 5 10 15
Ala Val Val Pro Leu Arg Arg Ser Val Ser Leu Ile Arg Ala Lys Val
20 25 30
Glu Pro Ser Glu Lys Thr Val Glu Ile Met Arg Lys Phe Ser Glu Gln
35 40 45
Tyr Ala Arg Arg Ser Glu Thr Tyr Phe Cys Val Asp Lys Gly Val Thr
50 55 60
Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Glu Thr Leu Gly Ala
65 70 75 80
Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Ala Ala
85 90 95
Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys Glu
100 105 110
Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly Arg
115 120 125
Glu Gln Ser Ile Thr Leu Asp Glu Ile Lys Val Ser Thr Ser Asn Leu
130 135 140
<210> 104
<211> 145
<212> PRT
<213> 陆地棉（Gossypium hirsutum）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 104
Met Thr Leu Gln Ala Ser Phe Phe Asn Phe Gly Ser Ile Ser Ser Leu
1 5 10 15
Pro Cys Pro Pro Arg Thr Ser Arg Gln Arg Phe Val Ile Arg Ala Lys
20 25 30
Val Glu Pro Ser Glu Lys Ser Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Thr Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val
85 90 95
Gly Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys Gly Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Asp Gln Ala Ile Thr Leu Glu Glu Ile Lys Glu Thr Thr Ala Asn
130 135 140
Met
145
<210> 105
<211> 145
<212> PRT
<213> 阿尔皮纳南芥（Arabis alpina）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 105
Met Asn Leu Gln Ala Val Ser Cys Ser Phe Gly Ser Val Ser Val Pro
1 5 10 15
Leu Val Ser Pro Arg Thr Pro Phe Arg Arg Phe Val Ile Gln Ala Lys
20 25 30
Ser Glu Pro Ser Glu Lys Ser Val Glu Leu Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Arg Ser Glu Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Glu His Lys Asp Ser Tyr Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Ala Ala Glu Val
85 90 95
Lys Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Lys Asp Gln Ser Ile Thr Leu Asp Glu Ile Lys Glu Thr Thr Ala Ser
130 135 140
Met
145
<210> 106
<211> 145
<212> PRT
<213> 白花羽扇豆（Lupinus angustifolius）
<220>
<221> MISC_FEATURE
<222> (1)..(145)
<223> 铁氧还蛋白-硫氧还蛋白还原酶
<400> 106
Met Thr Leu Gln Ala Ser Thr Phe Ala Val Ala Ile Pro Ser Ser Ala
1 5 10 15
Val Thr Pro Leu Arg Arg His Arg Asn Leu Phe Thr Val Arg Ala Gln
20 25 30
Val Glu Pro Thr Asp Lys Asn Val Glu Ile Met Arg Lys Phe Ser Glu
35 40 45
Gln Tyr Ala Arg Lys Ser Gly Thr Tyr Phe Cys Val Asp Lys Gly Val
50 55 60
Thr Ser Val Val Ile Lys Gly Leu Ala Asp His Lys Asp Ser Leu Gly
65 70 75 80
Ala Pro Leu Cys Pro Cys Arg His Tyr Asp Asp Lys Pro Ala Glu Ala
85 90 95
Ser Gln Gly Phe Trp Asn Cys Pro Cys Val Pro Met Arg Glu Arg Lys
100 105 110
Glu Cys His Cys Met Leu Phe Leu Thr Pro Asp Asn Asp Phe Ala Gly
115 120 125
Gln Glu Gln Thr Ile Thr Leu Asp Glu Ile Lys Glu Ala Thr Ala Asn
130 135 140
Met
145

Claims (15)

1.一种增加作物产量的方法，所述方法包括用至少一种铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列转化植物。
2.如权利要求1所述的方法，其中所述铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列包含SEQ ID NO：1，或编码选自SEQ ID NO：2和14-106的蛋白质。
3.一种植物，该植物基因组中稳定地纳入在植物中驱动表达的启动子，该启动子与铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列操作性地连接，其中所述启动子相对于所述铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列是异源的。
4.如权利要求3所述的植物，其中所述铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列包含SEQ ID NO：1，或编码选自SEQ ID NO：2和14-106的蛋白质。
5.如权利要求3-4中任一项所述植物的经转化的种子。
6.如权利要求3或4所述的植物，其中所述植物是单子叶植物。
7.如权利要求3或4所述的植物，其中所述植物是双子叶植物。
8.如权利要求1或2所述的方法，其中所述铁氧还蛋白-硫氧还蛋白还原酶蛋白编码序列由发育调节型启动子表达。
9.如权利要求8所述的方法，其中所述发育调节型启动子包含SEQ ID NO:5。
10.如权利要求3或4所述的植物，其中所述在植物中驱动表达的启动子是发育调节型启动子。
11.如权利要求10所述的植物，其中所述发育调节型启动子包含SEQ ID NO:5。
12.一种DNA构建体，所述DNA构建体包含操作性连接的，
a.在植物细胞中有功能的启动子，和
b.编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的核酸序列。
13.如权利要求12所述的DNA构建体，其中编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的所述核酸序列包含SEQ ID NO：1，或编码选自SEQ ID NO：2和14-106的蛋白质。
14.如权利要求12或13所述的DNA构建体，其中在植物细胞中有功能的所述启动子包含SEQ ID NO：5。
15.如权利要求12-14中任一项所述的DNA构建体，其中所述启动子相对于编码铁氧还蛋白-硫氧还蛋白还原酶蛋白的所述核酸序列是异源的。
CN201880059482.4A 2017-07-13 2018-07-12 使用铁氧还蛋白-硫氧还蛋白还原酶增加植物生长和产量 Pending CN111164206A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112779271A (zh) * 2021-03-31 2021-05-11 福建农林大学 水稻基因OsFd2及其在水稻抗稻瘟病中的应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109797153A (zh) * 2019-03-05 2019-05-24 内蒙古自治区农牧业科学院 弱化组织特异性启动子、提高植物光合作用效率的方法及其应用

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004035798A2 (en) * 2002-10-18 2004-04-29 Cropdesign N.V. Identification of e2f target genes and uses thereof
WO2005118820A2 (en) * 2004-05-27 2005-12-15 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
US20070011783A1 (en) * 1999-05-06 2007-01-11 Jingdong Liu Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
WO2013041969A2 (en) * 2011-09-21 2013-03-28 King Abdullah University Of Science And Technology Didemnin biosynthetic gene cluster in tistrella mobilis
CN103740718A (zh) * 2013-12-26 2014-04-23 中国农业科学院作物科学研究所 一种植物光诱导型基因启动子及其应用
WO2016182847A1 (en) * 2015-05-08 2016-11-17 Benson Hill Biosystems, Inc. Methods for increasing plant growth and yield by using an ictb sequence
WO2016210238A1 (en) * 2015-06-26 2016-12-29 Indigo Agriculture, Inc Penicillium endophyte compositions and methods for improved agronomic traits in plants

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US6015891A (en) 1988-09-09 2000-01-18 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene having a modified frequency of codon usage
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
EP0652965A1 (en) 1992-07-27 1995-05-17 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
JP2002529096A (ja) 1998-11-09 2002-09-10 パイオニア ハイ−ブレッド インターナショナル， インコーポレイテッド 転写アクチベータｌｅｃ１核酸、ポリペプチドおよびそれらの使用
US20050155114A1 (en) 2002-12-20 2005-07-14 Monsanto Company Stress-inducible plant promoters
US7642347B2 (en) 2006-06-23 2010-01-05 Monsanto Technology Llc Chimeric regulatory elements for gene expression in leaf mesophyll and bundle sheath cells
UY34014A (es) 2011-04-15 2012-11-30 Dow Agrosciences Llc Genes sintéticos para expresar proteínas en células de maíz, construcciones, plantas transgénicas, métodos para controlar pestes y composiciones
CN103981149A (zh) 2011-08-22 2014-08-13 拜尔作物科学公司 修饰植物基因组的方法和手段

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070011783A1 (en) * 1999-05-06 2007-01-11 Jingdong Liu Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
WO2004035798A2 (en) * 2002-10-18 2004-04-29 Cropdesign N.V. Identification of e2f target genes and uses thereof
WO2005118820A2 (en) * 2004-05-27 2005-12-15 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
WO2013041969A2 (en) * 2011-09-21 2013-03-28 King Abdullah University Of Science And Technology Didemnin biosynthetic gene cluster in tistrella mobilis
CN103740718A (zh) * 2013-12-26 2014-04-23 中国农业科学院作物科学研究所 一种植物光诱导型基因启动子及其应用
WO2016182847A1 (en) * 2015-05-08 2016-11-17 Benson Hill Biosystems, Inc. Methods for increasing plant growth and yield by using an ictb sequence
WO2016210238A1 (en) * 2015-06-26 2016-12-29 Indigo Agriculture, Inc Penicillium endophyte compositions and methods for improved agronomic traits in plants

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112779271A (zh) * 2021-03-31 2021-05-11 福建农林大学 水稻基因OsFd2及其在水稻抗稻瘟病中的应用

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