Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D519/00—Heterocyclic compounds containing more than one system of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring system not provided for in groups C07D453/00 or C07D455/00
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
  • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
  • C07D471/04—Ortho-condensed systems
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
  • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
  • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents

Definitions

• PD-1 Programmed cell death-1
• CD279 is a cell surface receptor expressed on activated T cells, natural killer T cells, B cells, and macrophages (Greenwald et al., Annu. Rev. Immunol.2005, 23:515–548; Okazaki and Honjo, Trends Immunol 2006, (4):195- 201).
• PD-1 functions as an intrinsic negative feedback sysxem to prevent the activation of T-cells, which in turn reduces autoimmunity and promotes self-tolerance.
• PD-1 is also known to play a critical role in the suppression of antigen-specific T cell response in diseases like cancer and viral infection (Sharpe et al., Nat. Immunol.20078, 239–245; Postow et al., J. Clinical Oncol.2015, 1-9).
• the structure of PD-1 consists of an extracellular immunoglobulin variable-like domain followed by a transmembrane region and an intracellular domain (Parry et al., Mol. Cell Biol. 2005, 9543–9553).
• the intracellular domain contains two phosphorylation sites located in an immunoreceptor tyrosine-based inhibitory motif and an immunoreceptor tyrosine-based switch motif, which suggests that PD-1 negatively regulates T cell receptor-mediated signals.
• PD-1 has two ligands, PD-L1 and PD-L2 (Parry et al., Mol. Cell Biol.2005, 9543–9553; Latchman et al., Nat. Immunol.2001, 2, 261–268), and they differ in their expression patterns.
• PD-L1 protein is upregulated on macrophages and dendritic cells in response to lipopolysaccharide and GM-CSF treatment, and on T cells and B cells upon T cell receptor and B cell receptor signaling.
• PD-L1 is also highly expressed on almost all tumor cells, and the expression is further increased after IFN- ⁇ treatment (Iwai et al., PNAS 2002, 99(19):12293-7; Blank et al., Cancer Res.2004, 64(3):1140-5).
• tumor PD-L1 expression status has been shown to be prognostic in multiple tumor types (Wang et al., Eur. J. Surg. Oncol.2015; Huang et al., Oncol.
• PD-L2 expression in contrast, is more restricted and is expressed mainly by dendritic cells (Nakae et al., J. Immunol.2006, 177:566- 73).
• Ligation of PD-1 with its ligands PD-L1 and PD-L2 on T cells delivers a signal that inhibits IL-2 and IFN- ⁇ production, as well as cell proliferation induced upon T cell receptor activation (Carter et al., Eur. J. Immunol.2002, 32(3):634-43; Freeman et al., J. Exp. Med.2000, 192(7):1027-34).
• the mechanism involves recruitment of SHP-2 or SHP-1 phosphatases to inhibit T cell receptor signaling such as Syk and Lck phosphorylation (Sharpe et al., Nat. Immunol.2007, 8, 239–245).
• Activation of the PD-1 signaling axis also attenuates PKC- ⁇ activation loop phosphorylation, which is necessary for the activation of NF- ⁇ B and AP1 pathways, and for cytokine production such as IL-2, IFN- ⁇ and TNF (Sharpe et al., Nat. Immunol.2007, 8, 239–245; Carter et al., Eur. J. Immunol.2002, 32(3):634-43; Freeman et al., J.
• PD-1-deficient mice have been shown to develop lupus-like glomerulonephritis and dilated cardiomyopathy (Nishimura et al., Immunity 1999, 11:141–151; Nishimura et al., Science 2001, 291:319–322).
• LCMV model of chronic infection it has been shown that PD-1/PD-L1 interaction inhibits activation, expansion and acquisition of effector functions of virus-specific CD8 T cells (Barber et al., Nature 2006, 439, 682-7).
• the present disclosure provides compounds of Formula (I): or a pharmaceutically acceptable salt thereof; wherein: R 1 is CH 3 or Cl; R 3 is H or CH 3 ; R 4 is H or CH 3 ; and R 5 is H or CH 3 .
• the present disclosure further provides a pharmaceutical composition comprising a compound described herein, or a pharmaceutically acceptable salt thereof, and one or more pharmaceutically acceptable excipient or carrier.
• the present disclosure further provides methods of inhibiting PD-1/PD-L1 interaction, said method comprising administering to a patient a compound described herein, or a pharmaceutically acceptable salt thereof.
• the present disclosure further provides methods of treating a disease or disorder associated with inhibition of PD-1/PD-L1 interaction, said method comprising administering to a patient in need thereof a therapeutically effective amount of a compound of described herein, or a pharmaceutically acceptable salt thereof.
• the present disclosure further provides methods of enhancing, stimulating and/or increasing the immune response in a patient, said method comprising administering to the patient in need thereof a therapeutically effective amount of a compound described herein, or a pharmaceutically acceptable salt thereof.
• the present disclosure provides a compound described herein, or a pharmaceutically acceptable salt thereof, for use in any of the methods described herein.
• the present disclosure provides use of a compound described herein, or a pharmaceutically acceptable salt thereof, for preparation of a medicament for use in any of the methods described herein.
• the present disclosure provides, inter alia, a compound of Formula (I): or a pharmaceutically acceptable salt thereof; wherein: R 1 is CH 3 or Cl; R 3 is H or CH 3 ; R 4 is H or CH 3 ; and R 5 is H or CH 3 .
• R 1 is CH 3 .
• R 1 is Cl.
• R 3 is H. Alternatively, in some embodiments, R 3 is CH 3 . In some embodiments, . In some embodiments, . In some embodiments, . In some embodiments of the preceding embodiment, R 4 is H. Alternatively, in some embodiments, R 4 is CH 3 . In some embodiments of the preceding embodiment, R 2 is . In some embodiments of the preceding embodiment, R 5 is H. Alternatively, in some embodiments, R 5 is CH 3 . , , or . In some embodiments: R 1 is CH 3 or Cl; when R 1 is CH 3 , then . In some embodiments, In some embodiments, .
• the compound is a compound selected from the compounds of Examples 1-16, or a pharmaceutically acceptable salt thereof. It is further appreciated that certain features of the disclosure, which are, for clarity, described in the context of separate embodiments, can also be provided in combination in a single embodiment (while the embodiments are intended to be combined as if written in multiply dependent form). Conversely, various features of the disclosure which are, for brevity, described in the context of a single embodiment, can also be provided separately or in any suitable subcombination. Thus, it is contemplated as features described as embodiments of the compounds of Formula (I) can be combined in any suitable combination. At various places in the present specification, certain features of the compounds are disclosed in groups or in ranges.
• the compounds described herein can be asymmetric (e.g., having one or more stereocenters). All stereoisomers, such as enantiomers and diastereomers, are intended unless otherwise indicated.
• Compounds of the present invention that contain asymmetrically substituted carbon atoms can be isolated in optically active or racemic forms. Methods on how to prepare optically active forms from optically inactive starting materials are known in the art, such as by resolution of racemic mixtures or by stereoselective synthesis.
• Cis and trans geometric isomers of the compounds of the present invention are described and may be isolated as a mixture of isomers or as separated isomeric forms. Resolution of racemic mixtures of compounds can be carried out by any of numerous methods known in the art. One method includes fractional recrystallization using a chiral resolving acid which is an optically active, salt-forming organic acid.
• Suitable resolving agents for fractional recrystallization methods are, e.g., optically active acids, such as the D and L forms of tartaric acid, diacetyltartaric acid, dibenzoyltartaric acid, mandelic acid, malic acid, lactic acid or the various optically active camphorsulfonic acids such as ⁇ -camphorsulfonic acid.
• optically active acids such as the D and L forms of tartaric acid, diacetyltartaric acid, dibenzoyltartaric acid, mandelic acid, malic acid, lactic acid or the various optically active camphorsulfonic acids such as ⁇ -camphorsulfonic acid.
• resolving agents suitable for fractional crystallization methods include stereoisomerically pure forms of ⁇ -methylbenzylamine (e.g., S and R forms, or diastereomerically pure forms), 2- phenylglycinol, norephedrine, ephedrine, N-methylephedrine, cyclohexylethylamine, 1,2- diaminocyclohexane and the like. Resolution of racemic mixtures can also be carried out by elution on a column packed with an optically active resolving agent (e.g., dinitrobenzoylphenylglycine). Suitable elution solvent composition can be determined by one skilled in the art.
• the compounds of the invention have the (R)-configuration. In other embodiments, the compounds have the (S)-configuration. In compounds with more than one chiral centers, each of the chiral centers in the compound may be independently (R) or (S), unless otherwise indicated.
• Compounds of the disclosure also include tautomeric forms. Tautomeric forms result from the swapping of a single bond with an adjacent double bond together with the concomitant migration of a proton. Tautomeric forms include prototropic tautomers which are isomeric protonation states having the same empirical formula and total charge.
• Example prototropic tautomers include ketone – enol pairs, amide - imidic acid pairs, lactam – lactim pairs, enamine – imine pairs, and annular forms where a proton can occupy two or more positions of a heterocyclic system, e.g., 1H- and 3H-imidazole, 1H-, 2H- and 4H- 1,2,4- triazole, 1H- and 2H- isoindole and 1H- and 2H-pyrazole.
• Tautomeric forms can be in equilibrium or sterically locked into one form by appropriate substitution.
• a compound described herein has one or more hydrogen atoms that are replaced by deuterium, or a pharmaceutically acceptable salt thereof.
• Compounds of the disclosure can also include all isotopes of atoms occurring in the intermediates or final compounds.
• Isotopes include those atoms having the same atomic number but different mass numbers.
• isotopes of hydrogen include tritium and deuterium.
• One or more constituent atoms of the compounds of the disclosure can be replaced or substituted with isotopes of the atoms in natural or non-natural abundance.
• the compound includes at least one deuterium atom.
• one or more hydrogen atoms in a compound of the present disclosure can be replaced or substituted by deuterium.
• the compound includes two or more deuterium atoms.
• the compound includes 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12 deuterium atoms.
• Synthetic methods for including isotopes into organic compounds are known in the art.
• the term, "compound,” as used herein is meant to include all tautomers and isotopes of the structures depicted.
• the term is also meant to refer to compounds of the disclosure, regardless of how they are prepared, e.g., synthetically, through biological process (e.g., metabolism or enzyme conversion), or a combination thereof. All compounds, and pharmaceutically acceptable salts thereof, can be found together with other substances such as water and solvents (e.g., hydrates and solvates) or can be isolated.
• the compounds described herein and salts thereof may occur in various forms and may, e.g., take the form of solvates, including hydrates.
• the compounds may be in any solid state form, such as a polymorph or solvate, so unless clearly indicated otherwise, reference in the specification to compounds and salts thereof should be understood as encompassing any solid state form of the compound.
• the compounds of the disclosure, or salts thereof are substantially isolated.
• substantially isolated is meant that the compound is at least partially or substantially separated from the environment in which it was formed or detected. Partial separation can include, e.g., a composition enriched in the compounds of the disclosure.
• Substantial separation can include compositions containing at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, at least about 97%, or at least about 99% by weight of the compounds of the disclosure, or salt thereof.
• the term “about” means ⁇ 10%. In some embodiments, the term “about” means ⁇ 5%.
• pharmaceutically acceptable is employed herein to refer to those compounds, materials, compositions and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.
• ambient temperature and “room temperature,” as used herein, are understood in the art, and refer generally to a temperature, e.g., a reaction temperature, that is about the temperature of the room in which the reaction is carried out, e.g., a temperature from about 20 oC to about 30 oC.
• a temperature e.g., a reaction temperature
• room temperature refer generally to a temperature, e.g., a reaction temperature, that is about the temperature of the room in which the reaction is carried out, e.g., a temperature from about 20 oC to about 30 oC.
• pharmaceutically acceptable salts refers to derivatives of the disclosed compounds wherein the parent compound is modified by converting an existing acid or base moiety to its salt form.
• Examples of pharmaceutically acceptable salts include, but are not limited to, mineral or organic acid salts of basic residues such as amines; alkali or organic salts of acidic residues such as carboxylic acids; and the like.
• the pharmaceutically acceptable salts of the present disclosure include the non-toxic salts of the parent compound formed, e.g., from non- toxic inorganic or organic acids.
• the pharmaceutically acceptable salts of the present disclosure can be synthesized from the parent compound which contains a basic or acidic moiety by conventional chemical methods.
• such salts can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, non-aqueous media like ether, ethyl acetate, alcohols (e.g., methanol, ethanol, iso-propanol or butanol) or acetonitrile (MeCN) are preferred.
• non-aqueous media like ether, ethyl acetate, alcohols (e.g., methanol, ethanol, iso-propanol or butanol) or acetonitrile (MeCN) are preferred.
• suitable salts are found in Remington's Pharmaceutical Sciences, 17 th Ed., (Mack Publishing Company, Easton, 1985), p.1418, Berge et al., J. Pharm.
• the compounds described herein include the N-oxide forms.
• Synthesis Compounds of the present disclosure, including salts thereof, can be prepared by the synthetic routes disclosed in Examples 1-16 infra.
• the reactions for preparing compounds of the disclosure can be carried out in suitable solvents which can be readily selected by one of skill in the art of organic synthesis. Suitable solvents can be substantially non-reactive with the starting materials (reactants), the intermediates or products at the temperatures at which the reactions are carried out, e.g., temperatures which can range from the solvent's freezing temperature to the solvent's boiling temperature.
• a given reaction can be carried out in one solvent or a mixture of more than one solvent.
• suitable solvents for a particular reaction step can be selected by the skilled artisan.
• Preparation of compounds of the disclosure can involve the protection and deprotection of various chemical groups. The need for protection and deprotection, and the selection of appropriate protecting groups, can be readily determined by one skilled in the art. The chemistry of protecting groups is described, e.g., in Kocienski, Protecting Groups, (Thieme, 2007); Robertson, Protecting Group Chemistry, (Oxford University Press, 2000); Smith et al., March's Advanced Organic Chemistry: Reactions, Mechanisms, and Structure, 6 th Ed.
• Reactions can be monitored according to any suitable method known in the art.
• product formation can be monitored by spectroscopic means, such as nuclear magnetic resonance spectroscopy (e.g., 1 H or 13 C), infrared spectroscopy, spectrophotometry (e.g., UV- visible), mass spectrometry or by chromatographic methods such as high performance liquid chromatography (HPLC) or thin layer chromatography (TLC).
• spectroscopic means such as nuclear magnetic resonance spectroscopy (e.g., 1 H or 13 C), infrared spectroscopy, spectrophotometry (e.g., UV- visible), mass spectrometry or by chromatographic methods such as high performance liquid chromatography (HPLC) or thin layer chromatography (TLC).
• the compounds of the present disclosure, or pharmaceutically acceptable salts thereof can inhibit the activity of PD-1/PD-L1 protein/protein interaction and, thus, are useful in treating diseases and disorders associated with activity of PD-1 and the diseases and disorders associated with PD-L1 including its interaction with other proteins such as PD-1 and B7-1 (CD80).
• the compounds of the present disclosure, or pharmaceutically acceptable salts thereof are useful for therapeutic administration to enhance, stimulate and/or increase immunity in cancer, chronic infection or sepsis, including enhancement of response to vaccination.
• the present disclosure provides a method for inhibiting the PD-1/PD-L1 protein/protein interaction.
• the method includes administering to an individual or a patient a compound described herein, or a pharmaceutically acceptable salt thereof.
• the compounds of the present disclosure can be used alone, in combination with other agents or therapies or as an adjuvant or neoadjuvant for the treatment of diseases or disorders, including cancer or infection diseases.
• any of the compounds of the disclosure including any of the embodiments thereof, may be used.
• the compounds of the present disclosure inhibit the PD-1/PD-L1 protein/protein interaction, resulting in a PD-1 pathway blockade.
• the blockade of PD-1 can enhance the immune response to cancerous cells and infectious diseases in mammals, including humans.
• the present disclosure provides treatment of an individual or a patient in vivo using a compound described herein or a salt thereof such that growth of cancerous tumors is inhibited.
• a compound described herein, or a salt thereof can be used to inhibit the growth of cancerous tumors.
• a compound described herein, or a salt thereof can be used in conjunction with other agents or standard cancer treatments, as described below.
• the present disclosure provides a method for inhibiting growth of tumor cells in vitro. The method includes contacting the tumor cells in vitro with a compound described herein, or of a salt thereof.
• the present disclosure provides a method for inhibiting growth of tumor cells in an individual or a patient.
• the method includes administering to the individual or patient in need thereof a therapeutically effective amount of a compound described herein, or a salt thereof.
• a method for treating cancer includes administering to a patient in need thereof, a therapeutically effective amount of a compound described herein, or a salt thereof.
• cancers include those whose growth may be inhibited using compounds of the disclosure and cancers typically responsive to immunotherapy.
• the present disclosure provides a method of enhancing, stimulating and/or increasing the immune response in a patient.
• the method includes administering to the patient in need thereof a therapeutically effective amount of a compound described herein, or a salt thereof.
• cancers include, but are not limited to, bone cancer, pancreatic cancer, skin cancer, cancer of the head or neck, cutaneous or intraocular malignant melanoma, uterine cancer, ovarian cancer, rectal cancer, cancer of the anal region, stomach cancer, testicular cancer, uterine cancer, carcinoma of the fallopian tubes, carcinoma of the endometrium, endometrial cancer, carcinoma of the cervix, carcinoma of the vagina, carcinoma of the vulva, Hodgkin's Disease, non-Hodgkin's lymphoma, cancer of the esophagus, cancer of the small intestine, cancer of the endocrine system, cancer of the thyroid gland, cancer of the parathyroid gland, cancer of the adrenal gland, sarcoma of soft tissue, cancer of the urethra, cancer of the penis, chronic or acute leukemias including acute myeloid leukemia, chronic myeloid leukemia, acute lymphoblastic leukemia, chronic lymphocytic leukemia
• cancers include melanoma (e.g., metastatic malignant melanoma, cutaneous melanoma), renal cancer (e.g., clear cell carcinoma), prostate cancer (e.g., hormone refractory prostate adenocarcinoma), breast cancer (e.g., breast invasive carcinoma), colon cancer, lung cancer (e.g., non-small cell lung cancer and small cell lung cancer), squamous cell head and neck cancer (e.g., squamous cell carcinoma of the head and neck), urothelial cancer (e.g., bladder cancer, nonmuscle invasive bladder cancer (NMIBC)) and cancers with high microsatellite instability (MSI high ).
• melanoma e.g., metastatic malignant melanoma, cutaneous melanoma
• renal cancer e.g., clear cell carcinoma
• prostate cancer e.g., hormone refractory prostate adenocarcinoma
• breast cancer e.g.,
• cancers include, but are not limited to, solid tumors (e.g., prostate cancer, colon cancer, esophageal cancer, endometrial cancer, ovarian cancer, uterine cancer, renal cancer, hepatic cancer, pancreatic cancer, gastric cancer, breast cancer, lung cancer, cancers of the head and neck, thyroid cancer, glioblastoma, sarcoma, bladder cancer, etc.), hematological cancers (e.g., lymphoma, leukemia such as acute lymphoblastic leukemia (ALL), acute myelogenous leukemia (AML), chronic lymphocytic leukemia (CLL), chronic myelogenous leukemia (CML), DLBCL, mantle cell lymphoma, Non-Hodgkin lymphoma (including relapsed or refractory NHL and recurrent follicular
• solid tumors e.g., prostate cancer, colon cancer, esophageal cancer, endometrial cancer, ovarian cancer
• cancers include, but are not limited to, cholangiocarcinoma, bile duct cancer, biliary tract cancer, triple negative breast cancer, rhabdomyosarcoma, small cell lung cancer, leiomyosarcoma, hepatocellular carcinoma, Ewing’s sarcoma, brain cancer, brain tumor, astrocytoma, neuroblastoma, neurofibroma, basal cell carcinoma, chondrosarcoma, epithelioid sarcoma, eye cancer, Fallopian tube cancer, gastrointestinal cancer, gastrointestinal stromal tumors, hairy cell leukemia, intestinal cancer, islet cell cancer, oral cancer, mouth cancer, throat cancer, laryngeal cancer, lip cancer, mesothelioma, neck cancer, nasal cavity cancer, ocular cancer, ocular melanoma, pelvic cancer, rectal cancer, renal cell carcinoma, salivary gland cancer, sinus cancer, spinal cancer, tongue cancer, tubular carcinoma, urethral cancer,
• the compounds of the present disclosure can be used to treat sickle cell disease and sickle cell anemia.
• diseases and indications that are treatable using the compounds of the present disclosure include, but are not limited to hematological cancers, sarcomas, lung cancers, gastrointestinal cancers, genitourinary tract cancers, liver cancers, bone cancers, nervous system cancers, gynecological cancers, and skin cancers.
• Exemplary hematological cancers include lymphomas and leukemias such as acute lymphoblastic leukemia (ALL), acute myelogenous leukemia (AML), acute promyelocytic leukemia (APL), chronic lymphocytic leukemia (CLL), chronic myelogenous leukemia (CML), diffuse large B-cell lymphoma (DLBCL), mantle cell lymphoma, Non-Hodgkin lymphoma (including relapsed or refractory NHL and recurrent follicular), Hodgkin lymphoma, myeloproliferative diseases (e.g., primary myelofibrosis (PMF), polycythemia vera (PV), and essential thrombocytosis (ET)), myelodysplasia syndrome (MDS), T-cell acute lymphoblastic lymphoma (T-ALL) and multiple myeloma (MM).
• ALL acute lymphoblastic leukemia
• AML acute mye
• Exemplary sarcomas include chondrosarcoma, Ewing’s sarcoma, osteosarcoma, rhabdomyosarcoma, angiosarcoma, fibrosarcoma, liposarcoma, myxoma, rhabdomyoma, rhabdosarcoma, fibroma, lipoma, hamartoma, and teratoma.
• Exemplary lung cancers include non-small cell lung cancer (NSCLC) (e.g., squamous cell NSCLC), small cell lung cancer, bronchogenic carcinoma (squamous cell, undifferentiated small cell, undifferentiated large cell, adenocarcinoma), alveolar (bronchiolar) carcinoma, bronchial adenoma, chondromatous hamartoma, and mesothelioma.
• NSCLC non-small cell lung cancer
• small cell lung cancer e.g., squamous cell NSCLC
• bronchogenic carcinoma squamous cell, undifferentiated small cell, undifferentiated large cell, adenocarcinoma
• alveolar (bronchiolar) carcinoma bronchial adenoma
• chondromatous hamartoma chondromatous hamartoma
• mesothelioma mesothelioma
• Exemplary gastrointestinal cancers include cancers of the esophagus (carcinoma, squamous cell carcinoma, adenocarcinoma, leiomyosarcoma, lymphoma), stomach (carcinoma, lymphoma, leiomyosarcoma, adenocarcinoma), pancreas (ductal adenocarcinoma, insulinoma, glucagonoma, gastrinoma, carcinoid tumors, vipoma), small bowel (adenocarcinoma, lymphoma, carcinoid tumors, Kaposi's sarcoma, leiomyoma, hemangioma, lipoma, neurofibroma, fibroma), large bowel (adenocarcinoma, tubular adenoma, villous adenoma, hamartoma, leiomyoma), and colorectal cancer (e.g., colorectal adeno
• Exemplary genitourinary tract cancers include cancers of the kidney (adenocarcinoma, Wilm's tumor [nephroblastoma]), bladder and urethra (squamous cell carcinoma, transitional cell carcinoma, adenocarcinoma), prostate (adenocarcinoma, sarcoma), and testis (seminoma, teratoma, embryonal carcinoma, teratocarcinoma, choriocarcinoma, sarcoma, interstitial cell carcinoma, fibroma, fibroadenoma, adenomatoid tumors, lipoma).
• the cancer is a urological cancer (e.g., papillary kidney carcinoma, testicular germ cell cancer, chromophobe renal cell carcinoma, clear cell renal carcinoma, or prostate adenocarcinoma).
• exemplary liver cancers include hepatoma (hepatocellular carcinoma), cholangiocarcinoma, hepatoblastoma, angiosarcoma, hepatocellular adenoma, and hemangioma.
• Exemplary bone cancers include, for example, osteogenic sarcoma (osteosarcoma), fibrosarcoma, malignant fibrous histiocytoma, chondrosarcoma, Ewing's sarcoma, malignant lymphoma (reticulum cell sarcoma), multiple myeloma, malignant giant cell tumor chordoma, osteochronfroma (osteocartilaginous exostoses), benign chondroma, chondroblastoma, chondromyxofibroma, osteoid osteoma, and giant cell tumors.
• osteogenic sarcoma osteosarcoma
• fibrosarcoma malignant fibrous histiocytoma
• chondrosarcoma chondrosarcoma
• Ewing's sarcoma malignant lymphoma
• multiple myeloma malignant giant cell tumor chordoma
• osteochronfroma osteocar
• Exemplary nervous system cancers include cancers of the skull (osteoma, hemangioma, granuloma, xanthoma, osteitis deformans), meninges (meningioma, meningiosarcoma, gliomatosis), brain (astrocytoma, meduoblastoma, glioma, ependymoma, germinoma (pinealoma), glioblastoma, glioblastoma multiform, oligodendroglioma, schwannoma, retinoblastoma, congenital tumors), and spinal cord (neurofibroma, meningioma, glioma, sarcoma), as well as neuroblastoma and Lhermitte-Duclos disease.
• skull osteoma, hemangioma, granuloma, xanthoma, osteitis de
• Exemplary gynecological cancers include cancers of the uterus (endometrial carcinoma), cervix (cervical carcinoma, pre -tumor cervical dysplasia), ovaries (ovarian carcinoma (serous cystadenocarcinoma, serous adenocarcinoma, mucinous cystadenocarcinoma, unclassified carcinoma), granulosa-thecal cell tumors, Sertoli-Leydig cell tumors, dysgerminoma, malignant teratoma), vulva (squamous cell carcinoma, intraepithelial carcinoma, adenocarcinoma, fibrosarcoma, melanoma), vagina (clear cell carcinoma, squamous cell carcinoma, botryoid sarcoma (embryonal rhabdomyosarcoma), and fallopian tubes (carcinoma).
• endometrial carcinoma endometrial carcinoma
• cervix cervical carcinoma, pre -
• Exemplary skin cancers include melanoma, basal cell carcinoma, squamous cell carcinoma (e.g., cutaneous squamous cell carcinoma), Kaposi's sarcoma, moles dysplastic nevi, lipoma, angioma, dermatofibroma, and keloids.
• diseases and indications that are treatable using the compounds of the present disclosure include, but are not limited to, sickle cell disease (e.g., sickle cell anemia), triple-negative breast cancer (TNBC), myelodysplastic syndromes, testicular cancer, bile duct cancer, esophageal cancer, and urothelial carcinoma.
• PD-1 pathway blockade with compounds of the present disclosure can also be used for treating infections such as viral, bacteria, fungus and parasite infections.
• the present disclosure provides a method for treating infections such as viral infections.
• the method includes administering to a patient in need thereof, a therapeutically effective amount of a compound described herein, or a salt thereof.
• viruses causing infections treatable by methods of the present disclosure include, but are not limited to, human immunodeficiency virus, human papillomavirus, influenza, hepatitis A, B, C or D viruses, adenovirus, poxvirus, herpes simplex viruses, human cytomegalovirus, severe acute respiratory syndrome virus, ebola virus, and measles virus.
• viruses causing infections treatable by methods of the present disclosure include, but are not limited to, hepatitis (A, B, or C), herpes virus (e.g., VZV, HSV-1, HAV-6, HSV-II, and CMV, Epstein Barr virus), adenovirus, influenza virus, flaviviruses, echovirus, rhinovirus, coxsackie virus, coronavirus, respiratory syncytial virus, mumps virus, rotavirus, measles virus, rubella virus, parvovirus, vaccinia virus, HTLV virus, dengue virus, papillomavirus, molluscum virus, poliovirus, rabies virus, JC virus, tuberculosis and arboviral encephalitis virus.
• herpes virus e.g., VZV, HSV-1, HAV-6, HSV-II, and CMV, Epstein Barr virus
• adenovirus e.g., adenovirus
• the present disclosure provides a method for treating bacterial infections.
• the method includes administering to a patient in need thereof, a therapeutically effective amount of a compound described herein, or a salt thereof.
• pathogenic bacteria causing infections treatable by methods of the disclosure include chlamydia, rickettsial bacteria, mycobacteria, staphylococci, streptococci, pneumococci, meningococci and conococci, klebsiella, proteus, serratia, pseudomonas, legionella, diphtheria, salmonella, bacilli, cholera, tetanus, botulism, anthrax, plague, leptospirosis, and Lyme's disease bacteria.
• the present disclosure provides a method for treating fungus infections.
• the method includes administering to a patient in need thereof, a therapeutically effective amount of a compound described herein, or a salt thereof.
• pathogenic fungi causing infections treatable by methods of the disclosure include Candida (albicans, krusei, glabrata, tropicalis, etc.), Cryptococcus neoformans, Aspergillus (fumigatus, niger, etc.), Genus Mucorales (mucor, absidia, rhizophus), Sporothrix schenkii, Blastomyces dermatitidis, Paracoccidioides brasiliensis, Coccidioides immitis and Histoplasma capsulatum.
• the present disclosure provides a method for treating parasite infections.
• the method includes administering to a patient in need thereof, a therapeutically effective amount of a compound described herein, or a salt thereof.
• pathogenic parasites causing infections treatable by methods of the disclosure include Entamoeba histolytica, Balantidium coli, Naegleriafowleri, Acanthamoeba sp., Giardia lambia, Cryptosporidium sp., Pneumocystis carinii, Plasmodium vivax, Babesia microti, Trypanosoma brucei, Trypanosoma cruzi, Leishmania donovani, Toxoplasma gondi, and Nippostrongylus brasiliensis.
• the present disclosure provides a method for treating neurodegenerative diseases or disorders.
• the method includes administering to a patient in need thereof, a therapeutically effective amount of a compound described herein, or a salt thereof.
• neurodegenerative diseases or disorders include Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, prion disease, Motor neurone diseases, Spinocerebellar ataxia and Spinal muscular atrophy. It is believed that compounds described herein, may possess satisfactory pharmacological profile and promising biopharmaceutical properties, such as toxicological profile, metabolism and pharmacokinetic properties, solubility, and permeability.
• biopharmaceutical properties is within the knowledge of a person skilled in the art, e.g., determination of cytotoxicity in cells or inhibition of certain targets or channels to determine potential toxicity.
• the phrase "therapeutically effective amount” refers to the amount of active compound or pharmaceutical agent that elicits the biological or medicinal response in a tissue, system, animal, individual or human that is being sought by a researcher, veterinarian, medical doctor or other clinician.
• treating refers to one or more of (1) inhibiting the disease; e.g., inhibiting a disease, condition or disorder in an individual who is experiencing or displaying the pathology or symptomatology of the disease, condition or disorder (i.e., arresting further development of the pathology and/or symptomatology); and (2) ameliorating the disease; e.g., ameliorating a disease, condition or disorder in an individual who is experiencing or displaying the pathology or symptomatology of the disease, condition or disorder (i.e., reversing the pathology and/or symptomatology) such as decreasing the severity of disease.
• the compounds of the disclosure are useful in preventing or reducing the risk of developing any of the diseases referred to herein; e.g., preventing or reducing the risk of developing a disease, condition or disorder in an individual who may be predisposed to the disease, condition or disorder but does not yet experience or display the pathology or symptomatology of the disease.
• Combination Therapies Immune-checkpoint therapies Compounds of the present disclosure, or pharmaceutically acceptable salts thereof, can be used in combination with one or more immune checkpoint inhibitors for the treatment of diseases, such as cancer or infections.
• immune checkpoint inhibitors include inhibitors against immune checkpoint molecules such as CBL-B, CD20, CD122, CD96, CD73, CD47, CSF1R, JAK, PI3K delta, PI3K gamma, TAM, arginase, HPK1, A2AR, B7-H3, B7-H4, BTLA, CTLA-4, LAG3, TIM3, TIGIT, CD112R, VISTA, PD-1, PD-L1 and PD-L2.
• the immune checkpoint molecule is a stimulatory checkpoint molecule selected from CD27, CD28, CD40, ICOS, OX40, GITR and CD137 (4-1BB).
• the immune checkpoint molecule is an inhibitory checkpoint molecule selected from A2AR, B7-H3, B7-H4, BTLA, CTLA-4, IDO, KIR, LAG3, PD-1, TIM3, TIGIT, and VISTA.
• the compounds provided herein can be used in combination with one or more agents selected from KIR inhibitors, TIGIT inhibitors, LAIR1 inhibitors, CD160 inhibitors, 2B4 inhibitors and TGF beta inhibitors.
• the compounds provided herein can be used in combination with one or more agonists of immune checkpoint molecules, e.g., OX40, CD27, GITR, and CD137 (also known as 4-1BB).
• the agonist of an immune checkpoint molecule is an agonist of OX40, CD27, CD28, GITR, ICOS, CD40, TLR7/8, and CD137 (also known as 4-1BB).
• the agonist of CD137 is urelumab.
• the agonist of CD137 is utomilumab.
• the agonist of an immune checkpoint molecule is an agonist of CD40.
• the agonist of CD40 is CP-870893, ADC-1013, CDX-1140, SEA-CD40, RO7009789, JNJ-64457107, APX-005M, or Chi Lob 7/4.
• the agonist of an immune checkpoint molecule is an agonist of ICOS. In some embodiments, the agonist of ICOS is GSK-3359609, JTX-2011, or MEDI- 570. In some embodiments, the agonist of an immune checkpoint molecule is an agonist of CD28. In some embodiments, the agonist of CD28 is theralizumab. In some embodiments, the agonist of an immune checkpoint molecule is an agonist of CD27. In some embodiments, the agonist of CD27 is varlilumab. In some embodiments, the agonist of an immune checkpoint molecule is an agonist of TLR7/8. In some embodiments, the agonist of TLR7/8 is MEDI9197.
• the inhibitor of an immune checkpoint molecule is anti-PD1 antibody, anti-PD-L1 antibody, or anti-CTLA-4 antibody. In some embodiments, the inhibitor of an immune checkpoint molecule is an inhibitor of PD-1, e.g., an anti-PD-1 monoclonal antibody.
• the anti-PD-1 monoclonal antibody is nivolumab, pembrolizumab (also known as MK-3475), pidilizumab, cemiplimab, spartalizumab, camrelizumab, cetrelimab, toripalimab, sintilimab, SHR-1210, PDR001, MGA012, PDR001, AB122, AMP-224, JTX-4014, BGB-108, BCD-100, BAT1306, LZM009, AK105, HLX10, or TSR-042.
• the anti-PD-1 monoclonal antibody is nivolumab or pembrolizumab.
• the anti-PD1 antibody is pembrolizumab. In some embodiments, the anti-PD-1 monoclonal antibody is MGA012. In some embodiments, the anti-PD1 antibody is SHR-1210. Other anti-cancer agent(s) include antibody therapeutics such as 4-1BB (e.g., urelumab, utomilumab). In some embodiments, the inhibitor of an immune checkpoint molecule is an inhibitor of PD-L1, e.g., an anti-PD-L1 monoclonal antibody.
• the anti-PD-L1 monoclonal antibody is BMS-935559, MEDI4736, MPDL3280A (also known as RG7446), durvalumab (Imfinzi®), atezolizumab (Tecentriq®), Avelumab (Bavencio®),MSB0010718C, tislelizumab, FAZ053, KN035, CS1001, SHR-1316, CBT-502, A167, STI-A101, CK-301, BGB-A333, MSB-2311, HLX20, or LY3300054.
• the anti-PD-L1 monoclonal antibody is MPDL3280A or MEDI4736.
• the inhibitor of an immune checkpoint molecule is an inhibitor of PD-1 and PD-L1, e.g., an anti-PD-1/PD-L1 bispecific antibody.
• the anti-PD-1/PD-L1 bispecific antibody is MCLA-136.
• the inhibitor is MCLA-145.
• the inhibitor of an immune checkpoint molecule is an inhibitor of CTLA-4, e.g., an anti-CTLA-4 antibody.
• the anti-CTLA-4 antibody is ipilimumab, tremelimumab, AGEN1884, or CP-675,206.
• the inhibitor of an immune checkpoint molecule is an inhibitor of PD-1 and CTLA-4, e.g., an anti-PD-1/CTLA-4 bispecific antibody.
• the anti-PD-1/CTLA-4 antibody is AK104.
• the inhibitor of an immune checkpoint molecule is an inhibitor of LAG3, e.g., an anti-LAG3 antibody.
• the anti-LAG3 antibody is BMS- 986016, LAG525, INCAGN2385, or eftilagimod alpha (IMP321).
• the inhibitor of an immune checkpoint molecule is an inhibitor of CD73. In some embodiments, the inhibitor of CD73 is oleclumab.
• the inhibitor of CD73 is MEDI9447.
• the inhibitor of an immune checkpoint molecule is an inhibitor of TIGIT.
• the inhibitor of TIGIT is OMP-31M32.
• the inhibitor of an immune checkpoint molecule is an inhibitor of VISTA.
• the inhibitor of VISTA is JNJ-61610588 or CA-170.
• the inhibitor of an immune checkpoint molecule is an inhibitor of B7-H3.
• the inhibitor of B7-H3 is enoblituzumab, MGD009, or 8H9.
• the inhibitor of an immune checkpoint molecule is an inhibitor of KIR.
• the inhibitor of KIR is lirilumab or IPH4102.
• the inhibitor of an immune checkpoint molecule is an inhibitor of A2aR.
• the inhibitor of A2aR is CPI-444.
• the inhibitor of an immune checkpoint molecule is an inhibitor of TGF-beta.
• the inhibitor of TGF-beta is trabedersen, galusertinib, or M7824.
• the inhibitor of an immune checkpoint molecule is an inhibitor of PI3K-gamma.
• the inhibitor of PI3K-gamma is IPI-549.
• the inhibitor of an immune checkpoint molecule is an inhibitor of CD47. In some embodiments, the inhibitor of CD47 is Hu5F9-G4 or TTI-621. In some embodiments, the inhibitor of an immune checkpoint molecule is an inhibitor of CD70. In some embodiments, the inhibitor of CD70 is cusatuzumab or BMS-936561. In some embodiments, the inhibitor of an immune checkpoint molecule is an inhibitor of TIM3, e.g., an anti-TIM3 antibody. In some embodiments, the anti-TIM3 antibody is INCAGN2390, MBG453, or TSR-022. In some embodiments, the agonist of an immune checkpoint molecule is an agonist of GITR, e.g., an anti-GITR antibody.
• the agonist is TRX518, MK-4166, INCAGN1876, MK-1248, AMG228, BMS-986156, GWN323, MEDI1873, or MEDI6469.
• the agonist of an immune checkpoint molecule is an agonist of OX40, e.g., OX40 agonist antibody or OX40L fusion protein.
• the anti- OX40 antibody is MEDI0562, MOXR-0916, PF-04518600, GSK3174998, BMS-986178, or 9B12.
• the OX40L fusion protein is MEDI6383.
• the inhibitor of an immune checkpoint molecule is an inhibitor of CD20, e.g., an anti-CD20 antibody.
• the anti-CD20 antibody is obinutuzumab or rituximab.
• the compounds of the present disclosure can be used in combination with bispecific antibodies.
• one of the domains of the bispecific antibody targets PD-1, PD-L1, CTLA-4, GITR, OX40, TIM3, LAG3, CD137, ICOS, CD3 or TGF ⁇ receptor.
• the bispecific antibody binds to PD-1 and PD-L1.
• the bispecific antibody that binds to PD-1 and PD-L1 is MCLA-136.
• the bispecific antibody binds to PD-L1 and CTLA-4. In some embodiments, the bispecific antibody that binds to PD-L1 and CTLA-4 is AK104.
• the compounds of the disclosure can be used in combination with one or more metabolic enzyme inhibitors.
• the metabolic enzyme inhibitor is an inhibitor of IDO1, TDO, or arginase. Examples of IDO1 inhibitors include epacadostat, NLG919, BMS-986205, PF-06840003, IOM2983, RG-70099 and LY338196. As provided throughout, the additional compounds, inhibitors, agents, etc.
• Cancer cell growth and survival can be impacted by dysfunction in multiple biological pathways.
• Targeting more than one signaling pathway (or more than one biological molecule involved in a given signaling pathway) may reduce the likelihood of drug-resistance arising in a cell population, or reduce the toxicity of treatment.
• the compounds of the present disclosure can be used in combination with one or more other therapies for the treatment of diseases, such as cancer or infections. Examples of diseases and indications treatable with combination therapies include those as described herein.
• cancers include solid tumors and non-solid tumors, such as liquid tumors, blood cancers.
• infections include viral infections, bacterial infections, fungus infections or parasite infections.
• the compounds of the present disclosure can be combined with one or more inhibitors of the following kinases for the treatment of cancer: Akt1, Akt2, Akt3, BCL2, CDK, TGF- ⁇ R, PKA, PKG, PKC, CaM-kinase, phosphorylase kinase, MEKK, ERK, MAPK, mTOR, EGFR, HER2, HER3, HER4, INS-R, IDH2, IGF-1R, IR-R, PDGF ⁇ R, PDGF ⁇ R, PI3K (alpha, beta, gamma, delta, and multiple or selective), CSF1R, KIT, FLK-II, KDR/FLK-1, FLK-4, flt-1, FGFR1, FGFR2, FGFR3, FGFR4, c
• the compounds of the present disclosure can be combined with one or more of the following inhibitors for the treatment of cancer or infections.
• inhibitors that can be combined with the compounds of the present disclosure for treatment of cancer and infections include an FGFR inhibitor (FGFR1, FGFR2, FGFR3 or FGFR4, e.g., pemigatinib (INCY54828), INCB62079), an EGFR (also known as ErB-1 or HER- 1) inhibitor (e.g., erlotinib, gefitinib, vandetanib, orsimertinib, cetuximab, necitumumab, or panitumumab), a VEGFR inhibitor or pathway blocker (e.g., bevacizumab, pazopanib, sunitinib, sorafenib, axitinib, regorafenib, ponatinib, cabozantinib, vandetanib,
• FGFR inhibitor
• the compounds of the present disclosure can be combined with a TLR7 agonist (e.g., imiquimod).
• TLR7 agonist e.g., imiquimod
• the compounds of the present disclosure can further be used in combination with other methods of treating cancers, for example by chemotherapy, irradiation therapy, tumor-targeted therapy, adjuvant therapy, immunotherapy or surgery.
• immunotherapy examples include cytokine treatment (e.g., interferons, GM-CSF, G-CSF, IL-2), CRS-207 immunotherapy, cancer vaccine, monoclonal antibody, bispecific or multi-specific antibody, antibody drug conjugate, adoptive T cell transfer, Toll receptor agonists, STING agonists, RIG-I agonists, oncolytic virotherapy and immunomodulating small molecules, including thalidomide or JAK1/2 inhibitor, PI3K ⁇ inhibitor and the like.
• the compounds can be administered in combination with one or more anti-cancer drugs, such as a chemotherapeutic agent.
• chemotherapeutics include any of: abarelix, aldesleukin, alemtuzumab, alitretinoin, allopurinol, altretamine, anastrozole, arsenic trioxide, asparaginase, azacitidine, bevacizumab, bexarotene, baricitinib, bleomycin, bortezomib, busulfan intravenous, busulfan oral, calusterone, capecitabine, carboplatin, carmustine, cetuximab, chlorambucil, cisplatin, cladribine, clofarabine, cyclophosphamide, cytarabine, dacarbazine, dactinomycin, dalteparin sodium, dasatinib, daunorubicin, decitabine, denileukin, denileukin diftitox, dexrazoxane, docetaxel
• anti-cancer agent(s) include antibody therapeutics such as trastuzumab (Herceptin), antibodies to costimulatory molecules such as CTLA-4 (e.g., ipilimumab), 4-1BB (e.g., urelumab, utomilumab), antibodies to PD-1 and PD-L1, or antibodies to cytokines (IL-10, TGF- ⁇ , etc.).
• trastuzumab Herceptin
• CTLA-4 e.g., ipilimumab
• 4-1BB e.g., urelumab, utomilumab
• PD-1 and PD-L1 antibodies to cytokines (IL-10, TGF- ⁇ , etc.
• antibodies to PD-1 and/or PD-L1 that can be combined with compounds of the present disclosure for the treatment of cancer or infections such as viral, bacteria, fungus and parasite infections include, but are not limited to nivolumab, pembrolizumab, atezolizumab, durvalumab, avelumab and SHR-1210.
• the compounds of the present disclosure can further be used in combination with one or more anti-inflammatory agents, steroids, immunosuppressants or therapeutic antibodies.
• the compounds described herein, or salts thereof can be combined with another immunogenic agent, such as cancerous cells, purified tumor antigens (including recombinant proteins, peptides, and carbohydrate molecules), cells, and cells transfected with genes encoding immune stimulating cytokines.
• tumor vaccines include peptides of melanoma antigens, such as peptides of gp100, MAGE antigens, Trp-2, MARTI and/or tyrosinase, or tumor cells transfected to express the cytokine GM-CSF.
• the compounds described herein, or salts thereof can be used in combination with a vaccination protocol for the treatment of cancer.
• the tumor cells are transduced to express GM-CSF.
• tumor vaccines include the proteins from viruses implicated in human cancers such as Human Papilloma Viruses (HPV), Hepatitis Viruses (HBV and HCV) and Kaposi's Herpes Sarcoma Virus (KHSV).
• HPV Human Papilloma Viruses
• HBV and HCV Hepatitis Viruses
• KHSV Kaposi's Herpes Sarcoma Virus
• the compounds of the present disclosure can be used in combination with tumor specific antigen such as heat shock proteins isolated from tumor tissue itself.
• the compounds described herein, or salts thereof can be combined with dendritic cells immunization to activate potent anti-tumor responses.
• the compounds of the present disclosure can be used in combination with bispecific macrocyclic peptides that target Fe alpha or Fe gamma receptor-expressing effectors cells to tumor cells.
• the compounds of the present disclosure can also be combined with macrocyclic peptides that activate host immune responsiveness.
• the compounds of the present disclosure can be used in combination with bone marrow transplant for the treatment of a variety of tumors of hematopoietic origin.
• the compounds described herein, or salts thereof can be used in combination with vaccines, to stimulate the immune response to pathogens, toxins, and self antigens. Examples of pathogens for which this therapeutic approach may be particularly useful, include pathogens for which there is currently no effective vaccine, or pathogens for which conventional vaccines are less than completely effective.
• HIV Hepatitis
• A, B, & C Hepatitis
• Influenza Herpes, Giardia, Malaria, Leishmania, Staphylococcus aureus, Pseudomonas Aeruginosa.
• Viruses causing infections treatable by methods of the present disclosure include, but are not limit to human papillomavirus, influenza, hepatitis A, B, C or D viruses, adenovirus, poxvirus, herpes simplex viruses, human cytomegalovirus, severe acute respiratory syndrome virus, ebola virus, measles virus, herpes virus (e.g., VZV, HSV-1, HAV-6, HSV-II, and CMV, Epstein Barr virus), flaviviruses, echovirus, rhinovirus, coxsackie virus, coronavirus, respiratory syncytial virus, mumpsvirus, rotavirus, measles virus, rubella virus, parvovirus, vaccinia virus, HTLV virus, dengue virus, papillomavirus, molluscum virus, poliovirus, rabies virus, JC virus and arboviral encephalitis virus.
• human papillomavirus influenza, hepatitis A
• Pathogenic bacteria causing infections treatable by methods of the disclosure include, but are not limited to, chlamydia, rickettsial bacteria, mycobacteria, staphylococci, streptococci, pneumococci, meningococci and conococci, klebsiella, proteus, serratia, pseudomonas, legionella, diphtheria, salmonella, bacilli, cholera, tetanus, botulism, anthrax, plague, leptospirosis, and Lyme's disease bacteria.
• Pathogenic fungi causing infections treatable by methods of the disclosure include, but are not limited to, Candida (albicans, krusei, glabrata, tropicalis, etc.), Cryptococcus neoformans, Aspergillus (fumigatus, niger, etc.), Genus Mucorales (mucor, absidia, rhizophus), Sporothrix schenkii, Blastomyces dermatitidis, Paracoccidioides brasiliensis, Coccidioides immitis and Histoplasma capsulatum.
• Candida albicans, krusei, glabrata, tropicalis, etc.
• Cryptococcus neoformans Aspergillus (fumigatus, niger, etc.)
• Genus Mucorales micor, absidia, rhizophus
• Sporothrix schenkii Blastomyces dermatitidis
• Paracoccidioides brasiliensis C
• Pathogenic parasites causing infections treatable by methods of the disclosure include, but are not limited to, Entamoeba histolytica, Balantidium coli, Naegleriafowleri, Acanthamoeba sp., Giardia lambia, Cryptosporidium sp., Pneumocystis carinii, Plasmodium vivax, Babesia microti, Trypanosoma brucei, Trypanosoma cruzi, Leishmania donovani, Toxoplasma gondi, and Nippostrongylus brasiliensis.
• compositions comprising a compound described herein, or a pharmaceutically acceptable salt thereof, or any of the embodiments thereof, and at least one pharmaceutically acceptable carrier or excipient.
• compositions can be prepared in a manner well known in the pharmaceutical art, and can be administered by a variety of routes, depending upon whether local or systemic treatment is indicated and upon the area to be treated.
• Administration may be topical (including transdermal, epidermal, ophthalmic and to mucous membranes including intranasal, vaginal and rectal delivery), pulmonary (e.g., by inhalation or insufflation of powders or aerosols, including by nebulizer; intratracheal or intranasal), oral or parenteral.
• Parenteral administration includes intravenous, intraarterial, subcutaneous, intraperitoneal intramuscular or injection or infusion; or intracranial, e.g., intrathecal or intraventricular, administration.
• Parenteral administration can be in the form of a single bolus dose, or may be, e.g., by a continuous perfusion pump.
• compositions and formulations for topical administration may include transdermal patches, ointments, lotions, creams, gels, drops, suppositories, sprays, liquids and powders.
• Conventional pharmaceutical carriers, aqueous, powder or oily bases, thickeners and the like may be necessary or desirable.
• This disclosure also includes pharmaceutical compositions which contain, as the active ingredient, the compound of the present disclosure or a pharmaceutically acceptable salt thereof, in combination with one or more pharmaceutically acceptable carriers or excipients.
• the composition is suitable for topical administration.
• the active ingredient is typically mixed with an excipient, diluted by an excipient or enclosed within such a carrier in the form of, e.g., a capsule, sachet, paper, or other container.
• a carrier in the form of, e.g., a capsule, sachet, paper, or other container.
• the excipient serves as a diluent, it can be a solid, semi-solid, or liquid material, which acts as a vehicle, carrier or medium for the active ingredient.
• compositions can be in the form of tablets, pills, powders, lozenges, sachets, cachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols (as a solid or in a liquid medium), ointments containing, e.g., up to 10% by weight of the active compound, soft and hard gelatin capsules, suppositories, sterile injectable solutions and sterile packaged powders.
• the active compound can be milled to provide the appropriate particle size prior to combining with the other ingredients. If the active compound is substantially insoluble, it can be milled to a particle size of less than 200 mesh.
• the particle size can be adjusted by milling to provide a substantially uniform distribution in the formulation, e.g., about 40 mesh.
• the compounds of the disclosure may be milled using known milling procedures such as wet milling to obtain a particle size appropriate for tablet formation and for other formulation types.
• Finely divided (nanoparticulate) preparations of the compounds of the disclosure can be prepared by processes known in the art see, e.g., WO 2002/000196.
• excipients include lactose, dextrose, sucrose, sorbitol, mannitol, starches, gum acacia, calcium phosphate, alginates, tragacanth, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup and methyl cellulose.
• the formulations can additionally include: lubricating agents such as talc, magnesium stearate and mineral oil; wetting agents; emulsifying and suspending agents; preserving agents such as methyl- and propylhydroxy-benzoates; sweetening agents; and flavoring agents.
• compositions of the disclosure can be formulated so as to provide quick, sustained or delayed release of the active ingredient after administration to the patient by employing procedures known in the art.
• the pharmaceutical composition comprises silicified microcrystalline cellulose (SMCC) and at least one compound described herein, or a pharmaceutically acceptable salt thereof.
• the silicified microcrystalline cellulose comprises about 98% microcrystalline cellulose and about 2% silicon dioxide w/w.
• the composition is a sustained release composition comprising at least one compound described herein, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable carrier or excipient.
• the composition comprises at least one compound described herein, or a pharmaceutically acceptable salt thereof, and at least one component selected from microcrystalline cellulose, lactose monohydrate, hydroxypropyl methylcellulose and polyethylene oxide. In some embodiments, the composition comprises at least one compound described herein, or a pharmaceutically acceptable salt thereof, and microcrystalline cellulose, lactose monohydrate and hydroxypropyl methylcellulose. In some embodiments, the composition comprises at least one compound described herein, or a pharmaceutically acceptable salt thereof, and microcrystalline cellulose, lactose monohydrate and polyethylene oxide. In some embodiments, the composition further comprises magnesium stearate or silicon dioxide. In some embodiments, the microcrystalline cellulose is Avicel PH102TM.
• the lactose monohydrate is Fast-flo 316TM.
• the hydroxypropyl methylcellulose is hydroxypropyl methylcellulose 2208 K4M (e.g., Methocel K4 M PremierTM) and/or hydroxypropyl methylcellulose 2208 K100LV (e.g., Methocel K00LVTM).
• the polyethylene oxide is polyethylene oxide WSR 1105 (e.g., Polyox WSR 1105TM).
• a wet granulation process is used to produce the composition.
• a dry granulation process is used to produce the composition.
• compositions can be formulated in a unit dosage form, each dosage containing from about 5 to about 1,000 mg (1 g), more usually about 100 mg to about 500 mg, of the active ingredient. In some embodiments, each dosage contains about 10 mg of the active ingredient. In some embodiments, each dosage contains about 50 mg of the active ingredient. In some embodiments, each dosage contains about 25 mg of the active ingredient.
• unit dosage forms refers to physically discrete units suitable as unitary dosages for human subjects and other mammals, each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect, in association with a suitable pharmaceutical excipient.
• the components used to formulate the pharmaceutical compositions are of high purity and are substantially free of potentially harmful contaminants (e.g., at least National Food grade, generally at least analytical grade, and more typically at least pharmaceutical grade).
• the composition is preferably manufactured or formulated under Good Manufacturing Practice standards as defined in the applicable regulations of the U.S. Food and Drug Administration.
• suitable formulations may be sterile and/or substantially isotonic and/or in full compliance with all Good Manufacturing Practice regulations of the U.S. Food and Drug Administration.
• the active compound may be effective over a wide dosage range and is generally administered in a therapeutically effective amount.
• the amount of the compound actually administered will usually be determined by a physician, according to the relevant circumstances, including the condition to be treated, the chosen route of administration, the actual compound administered, the age, weight, and response of the individual patient, the severity of the patient's symptoms and the like.
• the therapeutic dosage of a compound of the present disclosure can vary according to, e.g., the particular use for which the treatment is made, the manner of administration of the compound, the health and condition of the patient, and the judgment of the prescribing physician.
• the proportion or concentration of a compound of the disclosure in a pharmaceutical composition can vary depending upon a number of factors including dosage, chemical characteristics (e.g., hydrophobicity), and the route of administration.
• the compounds of the disclosure can be provided in an aqueous physiological buffer solution containing about 0.1 to about 10% w/v of the compound for parenteral administration.
• Some typical dose ranges are from about 1 ⁇ g/kg to about 1 g/kg of body weight per day. In some embodiments, the dose range is from about 0.01 mg/kg to about 100 mg/kg of body weight per day.
• the dosage is likely to depend on such variables as the type and extent of progression of the disease or disorder, the overall health status of the particular patient, the relative biological efficacy of the compound selected, formulation of the excipient, and its route of administration. Effective doses can be extrapolated from dose-response curves derived from in vitro or animal model test systems.
• the principal active ingredient is mixed with a pharmaceutical excipient to form a solid preformulation composition containing a homogeneous mixture of a compound of the present disclosure.
• a solid preformulation composition containing a homogeneous mixture of a compound of the present disclosure.
• the active ingredient is typically dispersed evenly throughout the composition so that the composition can be readily subdivided into equally effective unit dosage forms such as tablets, pills and capsules.
• This solid preformulation is then subdivided into unit dosage forms of the type described above containing from, e.g., about 0.1 to about 1000 mg of the active ingredient of the present disclosure.
• the tablets or pills of the present disclosure can be coated or otherwise compounded to provide a dosage form affording the advantage of prolonged action.
• the tablet or pill can comprise an inner dosage and an outer dosage component, the latter being in the form of an envelope over the former.
• the two components can be separated by an enteric layer which serves to resist disintegration in the stomach and permit the inner component to pass intact into the duodenum or to be delayed in release.
• enteric layers or coatings such materials including a number of polymeric acids and mixtures of polymeric acids with such materials as shellac, cetyl alcohol and cellulose acetate.
• compositions for inhalation or insufflation include solutions and suspensions in pharmaceutically acceptable, aqueous or organic solvents, or mixtures thereof, and powders.
• the liquid or solid compositions may contain suitable pharmaceutically acceptable excipients as described supra.
• the compositions are administered by the oral or nasal respiratory route for local or systemic effect.
• compositions can be nebulized by use of inert gases. Nebulized solutions may be breathed directly from the nebulizing device or the nebulizing device can be attached to a face mask, tent, or intermittent positive pressure breathing machine. Solution, suspension, or powder compositions can be administered orally or nasally from devices which deliver the formulation in an appropriate manner.
• Topical formulations can contain one or more conventional carriers.
• ointments can contain water and one or more hydrophobic carriers selected from, e.g., liquid paraffin, polyoxyethylene alkyl ether, propylene glycol, white Vaseline, and the like.
• Carrier compositions of creams can be based on water in combination with glycerol and one or more other components, e.g., glycerinemonostearate, PEG-glycerinemonostearate and cetylstearyl alcohol.
• Gels can be formulated using isopropyl alcohol and water, suitably in combination with other components such as, e.g., glycerol, hydroxyethyl cellulose, and the like.
• topical formulations contain at least about 0.1, at least about 0.25, at least about 0.5, at least about 1, at least about 2 or at least about 5 wt. % of the compound of the disclosure.
• the topical formulations can be suitably packaged in tubes of, e.g., 100 g which are optionally associated with instructions for the treatment of the select indication, e.g., psoriasis or other skin condition.
• the amount of compound or composition administered to a patient will vary depending upon what is being administered, the purpose of the administration, such as prophylaxis or therapy, the state of the patient, the manner of administration and the like.
• compositions can be administered to a patient already suffering from a disease in an amount sufficient to cure or at least partially arrest the symptoms of the disease and its complications. Effective doses will depend on the disease condition being treated as well as by the judgment of the attending clinician depending upon factors such as the severity of the disease, the age, weight and general condition of the patient and the like.
• compositions administered to a patient can be in the form of pharmaceutical compositions described above. These compositions can be sterilized by conventional sterilization techniques, or may be sterile filtered. Aqueous solutions can be packaged for use as is, or lyophilized, the lyophilized preparation being combined with a sterile aqueous carrier prior to administration.
• the pH of the compound preparations typically will be between 3 and 11, more preferably from 5 to 9 and most preferably from 7 to 8. It will be understood that use of certain of the foregoing excipients, carriers or stabilizers will result in the formation of pharmaceutical salts.
• the therapeutic dosage of a compound of the present disclosure can vary according to, e.g., the particular use for which the treatment is made, the manner of administration of the compound, the health and condition of the patient, and the judgment of the prescribing physician.
• the proportion or concentration of a compound of the disclosure in a pharmaceutical composition can vary depending upon a number of factors including dosage, chemical characteristics (e.g., hydrophobicity), and the route of administration.
• the compounds of the disclosure can be provided in an aqueous physiological buffer solution containing about 0.1 to about 10% w/v of the compound for parenteral administration. Some typical dose ranges are from about 1 ⁇ g/kg to about 1 g/kg of body weight per day.
• the dose range is from about 0.01 mg/kg to about 100 mg/kg of body weight per day.
• the dosage is likely to depend on such variables as the type and extent of progression of the disease or disorder, the overall health status of the particular patient, the relative biological efficacy of the compound selected, formulation of the excipient, and its route of administration. Effective doses can be extrapolated from dose-response curves derived from in vitro or animal model test systems. V. Labeled Compounds and Assay Methods The compounds of the present disclosure can further be useful in investigations of biological processes in normal and abnormal tissues.
• another aspect of the present disclosure relates to labeled compounds of the disclosure (radio-labeled, fluorescent-labeled, etc.) that would be useful not only in imaging techniques but also in assays, both in vitro and in vivo, for localizing and quantitating PD-1 or PD-L1 protein in tissue samples, including human, and for identifying PD-L1 ligands by inhibition binding of a labeled compound.
• the present disclosure includes PD-1/PD-L1 binding assays that contain such labeled compounds.
• the present disclosure further includes isotopically-labeled compounds of the disclosure.
• an “isotopically” or “radio-labeled” compound is a compound of the disclosure where one or more atoms are replaced or substituted by an atom having an atomic mass or mass number different from the atomic mass or mass number typically found in nature (i.e., naturally occurring).
• Suitable radionuclides that may be incorporated in compounds of the present disclosure include but are not limited to 3 H (also written as T for tritium), 11 C, 13 C, 14 C, 13 N, 15 N, 15 O, 17 O, 18 O, 18 F, 35 S, 36 Cl, 82 Br, 75 Br, 76 Br, 77 Br, 123 I, 124 I, 125 I and 131 I.
• one or more hydrogen atoms in a compound of the present disclosure can be replaced by deuterium atoms.
• One or more constituent atoms of the compounds presented herein can be replaced or substituted with isotopes of the atoms in natural or non-natural abundance.
• the compound includes at least one deuterium atom.
• the compound includes two or more deuterium atoms.
• the compound includes 1-2, 1-3, 1-4, 1-5, or 1-6 deuterium atoms.
• all of the hydrogen atoms in a compound can be replaced or substituted by deuterium atoms.
• substitution with heavier isotopes may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in vivo half-life or reduced dosage requirements, and hence may be preferred in some circumstances.
• substitution at one or more metabolism sites may afford one or more of the therapeutic advantages.
• the radionuclide that is incorporated in the instant radio-labeled compounds will depend on the specific application of that radio-labeled compound.
• radio-labeled or “labeled compound” is a compound that has incorporated at least one radionuclide.
• the radionuclide is selected from the group consisting of 3 H, 14 C, 125 I, 35 S and 82 Br.
• the present disclosure can further include synthetic methods for incorporating radio- isotopes into compounds of the disclosure.
• a labeled compound of the disclosure can be used in a screening assay to identify and/or evaluate compounds.
• a newly synthesized or identified compound i.e., test compound
• a test compound which is labeled can be evaluated for its ability to bind a PD-L1 protein by monitoring its concentration variation when contacting with the PD-L1 protein, through tracking of the labeling.
• a test compound (labeled) can be evaluated for its ability to reduce binding of another compound which is known to bind to a PD-L1 protein (i.e., standard compound).
• test compound to compete with the standard compound for binding to the PD-L1 protein directly correlates to its binding affinity.
• standard compound is labeled and test compounds are unlabeled. Accordingly, the concentration of the labeled standard compound is monitored in order to evaluate the competition between the standard compound and the test compound, and the relative binding affinity of the test compound is thus ascertained.
• Kits The present disclosure also includes pharmaceutical kits useful, e.g., in the treatment or prevention of diseases or disorders associated with the activity of PD-L1 including its interaction with other proteins such as PD-1 and B7-1 (CD80), such as cancer or infections, which include one or more containers containing a pharmaceutical composition comprising a therapeutically effective amount of a compound described herein.
• kits can further include one or more of various conventional pharmaceutical kit components, such as, e.g., containers with one or more pharmaceutically acceptable carriers, additional containers, etc., as will be readily apparent to those skilled in the art.
• Instructions, either as inserts or as labels, indicating quantities of the components to be administered, guidelines for administration, and/or guidelines for mixing the components, can also be included in the kit.
• Step 2 5-bromo-3-(2,2-difluoroacetamido)picolinamide
• To reactor 1 was charged sequentially 3-amino-5-bromopicolinamide (2.0 kg, 9.3 mol), DCM (8.0 L), and triethylamine (2.8 kg, 27.8 mol).
• the mixture in reactor 1 was cooled to 5-10 o C.
• To reactor 2 was charged sequentially difluroacetic acid (2.1 kg, 21.4 mol), DCM (2.0 L), and DMF (50 mL).
• the mixture in reactor 1 was slowly added to reactor 2.
• the reaction temperature was controlled to below 15 o C during the addition. After addition, the reaction was stirred at 15-20 o C for 4 hrs.
• Step 3 7-bromo-2-(difluoromethyl)pyrido[3,2-d]pyrimidin-4-ol
• 5-bromo-3-(2,2- difluoroacetamido)picolinamide 870 g, 3.0 mol
• ethanol 2.0 L
• water 0.5 L
• sodium hydroxide 118 g, 3.0 mol
• Step 4 2-(difluoromethyl)-7-vinylpyrido[3,2-d]pyrimidin-4-ol
• a reactor was charged sequentially 7-bromo-2-(difluoromethyl)pyrido[3,2- d]pyrimidin-4-ol (1.3 kg, 4.7 mol), ethanol (6.0 L), water (6.0 L), potassium carbonate (1.9 kg, 14.1 mol), [1,1'-Bis(diphenylphosphino)ferrocene]palladium(II) dichloride (130 g, 10 wt. %), and vinylboronic acid pinacol ester (0.9 kg, 6.1 mol). The mixture was stirred at 80-85 o C for 60-70 hrs.
• reaction mixture was filtered through a pad of diatomaceous earth and rinsed with ethyl acetate (0.5 L). Then the filtrate was adjusted by conc. HCl solution to pH 2-3 before extracted with ethyl acetate three times (3x10.0 L). The organic layers were combined and washed with brine (4.0 L), and concentrated to afford a crude residue. To the residue was added petroleum ether (2.0 L). The mixture was stirred at room temperature for 2 hrs. Then the suspension was filtered and the solid was collected as a yellow solid (500 g, 50% yield).
• Step 5 4-chloro-2-(difluoromethyl)-7-vinylpyrido[3,2-d]pyrimidine
• 2-(difluoromethyl)-7- vinylpyrido[3,2-d]pyrimidin-4-ol 200 g, 0.9 mol
• phosphoryl chloride 206 g, 1.35 mol
• N,N-dimethylaniline 190 g, 1.35 mol
• the reaction mixture was poured into iced water (1.0 L).
• Step 6 N-(3-bromo-2-methylphenyl)-2-(difluoromethyl)-7-vinylpyrido[3,2-d]pyrimidin-4- amine
• a reactor was charged sequentially tert-butanol (2.5 L), 4-chloro-2- (difluoromethyl)-7-vinylpyrido[3,2-d]pyrimidine (250 g, 1.04 mol) and 3-bromo-2- methylaniline (188 g, 1.25 mol).
• the mixture was stirred at 50 o C overnight.
• Then the reaction was cooled to room temperature.
• the suspension was filtered and the solid was collected (375 g, 92% yield) and used without further purification.
• Step 7 4-((3-bromo-2-methylphenyl)amino)-2-(difluoromethyl)pyrido[3,2-d]pyrimidine-7- carbaldehyde
• THF 7.0 L
• water 2.0 L
• sodium periodate 620 g, 2.8 mol
• 2,6-dimethylpyridine 205 g, 1.9 mol
• osmium tetraoxide 2.0 g, 0.09 mol
• N-(3-bromo-2- methylphenyl)-2-(difluoromethyl)-7-vinylpyrido[3,2-d]pyrimidin-4-amine 370 g, 0.96 mol
• Step 2 tert-butyl 1-methyl-1,4,6,7-tetrahydro-5H-imidazo[4,5-c]pyridine-5-carboxylate
• a solution of 1-methyl-4,5,6,7-tetrahydro-1H-imidazo[4,5-c]pyridine (Accela, cat#SY032476: 2.0 g), (Boc) 2 O (3.38 mL) in dichloromethane (60 mL) was stirred at room temperature for 1 h. The reaction was quenched with saturated aqueous NaHCO 3 solution, and extracted with ethyl acetate. The combined organic layers were washed with brine, dried over Na2SO4 and filtered. The filtrate was concentrated under reduced pressure.
• Step 3 The crude product was used in Step 3 without further purification.
• LCMS calculated for C 12 H 20 N 3 O 2 (M+H) + : m/z 238.2; found 238.2.
• Step 3 5-(tert-butyl) 2-methyl 1-methyl-1,4,6,7-tetrahydro-5H-imidazo[4,5-c]pyridine-2,5- dicarboxylate
• n-Butyllithium in hexanes 2.5 M, 7.00 mL
• Step 3 was added to a cold (-78 °C) solution of the crude product tert-butyl 1-methyl-1,4,6,7-tetrahydro-5H-imidazo[4,5-c]pyridine-5- carboxylate from Step 2 (3.46 g) in tetrahydrofuran (60.0 mL).
• Step 4 tert-butyl 2-((2-chloro-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2- yl)phenyl)carbamoyl)-1-methyl-1,4,6,7-tetrahydro-5H-imidazo[4,5-c]pyridine-5-carboxylate
• Potassium tert-butoxide solution 1.0 M in THF, 16.1 mL
• Step 6 methyl (R)-4-(2-(2-((2-chloro-3'-((2-(difluoromethyl)-7-((3-hydroxypyrrolidin-1- yl)methyl)pyrido[3,2-d]pyrimidin-4-yl)amino)-2'-methyl-[1,1'-biphenyl]-3-yl)carbamoyl)-1- methyl-1,4,6,7-tetrahydro-5H-imidazo[4,5-c]pyridin-5-yl)ethyl)bicyclo[2.2.1]heptane-1- carboxylate
• Step 7 (R)-4-(2-(2-((2-chloro-3'-((2-(difluoromethyl)-7-((3-hydroxypyrrolidin-1- yl)methyl)pyrido[3,2-d]pyrimidin-4-yl)amino)-2'-methyl-[1,1'-biphenyl]-3-yl)carbamoyl)-1- methyl-1,4,6,7-tetrahydro-5H-imidazo[4,5-c]pyridin-5-yl)ethyl)bicyclo[2.2.1]heptane-1- carboxylic acid To a solution of methyl (R)-4-(2-(2-((2-chloro-3'-((2-(difluoromethyl)-7-((3- hydroxypyrrolidin-1-yl)methyl)pyrido[3,2-d]pyrimidin-4-yl)amino)-2'-methyl-[1,1'- biphenyl]-3-yl)carbam
• Step 2 tert-butyl 2-((2-chloro-3'-((2-(difluoromethyl)-7-(hydroxymethyl)pyrido[3,2- d]pyrimidin-4-yl)amino)-2'-methyl-[1,1'-biphenyl]-3-yl)carbamoyl)-1-methyl-1,4,6,7- tetrahydro-5H-imidazo[4,5-c]pyridine-5-carboxylate
• 4-((3-bromo-2-methylphenyl)amino)-2-(difluoromethyl)pyrido[3,2- d]pyrimidin-7-yl)methanol (767 mg)
• Step 3 methyl 4-(2-(2-((2-chloro-3'-((2-(difluoromethyl)-7-(hydroxymethyl)pyrido[3,2- d]pyrimidin-4-yl)amino)-2'-methyl-[1,1'-biphenyl]-3-yl)carbamoyl)-1-methyl-1,4,6,7- tetrahydro-5H-imidazo[4,5-c]pyridin-5-yl)ethyl)bicyclo[2.2.1]heptane-1-carboxylate To a solution of tert-butyl 2-((2-chloro-3'-((2-(difluoromethyl)-7- (hydroxymethyl)pyrido [3,2-d]pyrimidin-4-yl)amino)-2'-methyl-[1,1'-biphenyl]-3- yl)carbamoyl)-1-methyl-1,4,6,7-tetrahydro-5H-imidazo[4,5-c
• Step 4 methyl 4-(2-(2-((2-chloro-3'-((2-(difluoromethyl)-7-formylpyrido[3,2-d]pyrimidin-4- yl)amino)-2'-methyl-[1,1'-biphenyl]-3-yl)carbamoyl)-1-methyl-1,4,6,7-tetrahydro-5H- imidazo[4,5-c]pyridin-5-yl)ethyl)bicyclo[2.2.1]heptane-1-carboxylate To a solution of methyl 4-(2-(2-((2-chloro-3'-((2-(difluoromethyl)-7- (hydroxymethyl)pyrido[3,2-d]pyrimidin-4-yl)amino)-2'-methyl-[1,1'-biphenyl]-3- yl)carbamoyl)-1-methyl-1,4,6,7-tetrahydro-5H-imidazo[4,5-c]pyri
• Step 5 (S)-4-(2-(2-((2-chloro-3'-((2-(difluoromethyl)-7-((3-hydroxypyrrolidin-1- yl)methyl)pyrido[3,2-d]pyrimidin-4-yl)amino)-2'-methyl-[1,1'-biphenyl]-3-yl)carbamoyl)-1- methyl-1,4,6,7-tetrahydro-5H-imidazo[4,5-c]pyridin-5-yl)ethyl)bicyclo[2.2.1]heptane-1- carboxylic acid To a solution of methyl 4-(2-(2-((2-chloro-3'-((2-(difluoromethyl)-7- formylpyrido[3,2-d]pyrimidin-4-yl)amino)-2'-methyl-[1,1'-biphenyl]-3-yl)carbamoyl)-1- methyl-1,4,6,7-tetra
• Example 7 4-(2-(2-((2-chloro-3'-((2-(difluoromethyl)-7-(pyrrolidin-1- ylmethyl)pyrido[3,2-d]pyrimidin-4-yl)amino)-2'-methyl-[1,1'-biphenyl]-3- yl)carbamoyl)-1-methyl-1,4,6,7-tetrahydro-5H-imidazo[4,5-c]pyridin-5- yl)ethyl)bicyclo[2.2.1]heptane-1-carboxylic acid
• This compound was prepared using similar procedures as described for Example 3 with pyrrolidine replacing (S)-pyrrolidin-3-ol (Step 5).
• This compound was prepared using similar procedures as described for Example 3 with (1S,2R)-2-aminocyclopentan-1-ol replacing (S)-pyrrolidin-3-ol (Step 5).
• the final concentration of DMSO in the assay was 1%.
• the assays were carried out at 25° C in the PBS buffer (pH 7.4) with 0.05% Tween-20 and 0.1% BSA.
• Recombinant human PD-L1 protein (19-238) with a His- tag at the C-terminus was purchased from AcroBiosystems (PD1-H5229).
• Recombinant human PD-1 protein (25-167) with Fc tag at the C-terminus was also purchased from AcroBiosystems (PD1-H5257).
• PD-L1 and PD-1 proteins were diluted in the assay buffer and 10 ⁇ L was added to the plate well. Plates were centrifuged and proteins were preincubated with inhibitors for 40 minutes.
• Example B Src Homology region 2 Domain-containing Phosphatase (SHP) Assay U2OS/PD-L1 cells (DiscoveRx Corporation) were maintained in McCoy’s 5A medium with addition of 10% FBS, 0.25 ⁇ g/mL Puromycin. After removing the culture media, the cell medium was replaced with assay medium (RPMI1640 medium with 1% FBS). The U2OS/PD-L1 cells were then added in 384-well black clear bottom assay plate (CELLCOAT® Tissue Culture Plates, Greiner Bio-One) at 5000 cells per well in 20 ⁇ L assay medium.
• SHP Src Homology region 2 Domain-containing Phosphatase
• Test compounds were prepared by serial dilution in DMSO and 125 nL compound were first transferred to the 384 REMP plate well (Thermofisher) by ECHO liquid handler (Labcyte) followed with addition of 27.5 ⁇ L assay medium.5 ⁇ L/well compounds in the assay medium were transferred to the cell plate with 0.05% DMSO in the final assay at 0.25 ⁇ M.
• Jurkat-PD-1-SHP cells (DiscoveRx Corporation) were cultured in RPMI1640 medium supplemented with 10% FBS, 250 ⁇ g/mL Hygromycin B, 500 ⁇ g/mL G418.
• IC 50 determination was performed by fitting the curve of percentage of control activity versus the log of the compound concentration using the GraphPad Prism 6.0 software.
• Example C Nuclear Factor of Activated T-cells (NFAT) Assay PD-L1 aAPC/CHO-K1cells (Promega) were maintained in F-12 medium with addition of 10% FBS, 200 ⁇ g/mL Hygromycin B, 250 ⁇ g/mL Geneticin (G418).
• Jurkat-PD-1- NFAT effector cells (Promega) were cultured in RPMI 1640 medium supplemented with 10% FBS, 100 ⁇ g/mL Hygromycin B, 500 ⁇ g/mL G418.
• the culture media of PD-L1 aAPC/CHO-K1 cells were first replaced with assay medium (RPMI1640 medium with 1% FBS).
• assay medium RPMI1640 medium with 1% FBS.
• the PD-L1 aAPC/CHO-K1cells were then added in a white 384-well white clear bottom assay plate (CELLCOAT® Tissue Culture Plates, Greiner Bio-One) at 8000 per well in 10 ⁇ L assay medium.
• Test compounds were prepared by serial dilution in DMSO and 0.8 ⁇ L test compounds in DMSO were first transferred to the 384 REMP plate well (Thermofisher) by PlateMate Plus (Thermofisher) followed with addition of 50 ⁇ L plating medium.5 ⁇ L compounds in the assay medium were transferred to the cells with 0.4% DMSO in the final assay at 2 ⁇ M. After removing the culture media, 10,000 Jurkat-PD-1- NFAT effector cells in 5 ⁇ L assay medium was dispensed into each well. The assay plate was incubated at 37 °C, 5% CO 2 for 24 hours.
• Example D PD-L1 Whole Blood Internalization Assay
• normal human blood Biological Specialty Corp, Colmar. PA
• 1 ng/mL human interferon ⁇ R&D Systems Inc. Minn. MN
• 2mL Assay Block Corning, Corning NY
• PD-L1 MIH1, eBioscience; or BD Biosciences San Jose, CA
• CD14 Life Technologies, Carlsbad, CA
• Whole Blood/red cells were lysed/fixed (lysis buffer BD Biosciences) for 5 minutes at 37 0 C in the dark and then centrifuged at 1600 RPM for 5 minutes. Cells were resuspended in Stain Buffer (BD Bioscience, San Jose, CA) and transferred into 96 well round bottom plates (Corning). Cells were gated on CD14+ (BD Biosciences) and PD-L1 expression determined by mean fluorescence intensity (MFI) (BD LSRFortessaTM X-20).
• MFI mean fluorescence intensity
• test compounds were administered to male Sprague Dawley rats or male and female Cynomolgus monkeys intravenously or via oral gavage.
• test compounds were dosed at 0.5 to 1 mg/kg using a formulation of 10% dimethylacetamide (DMAC) in acidified saline via IV bolus for rat and 5 min or 10 min IV infusion for monkey.
• DMAC dimethylacetamide
• test compounds were dosed at 1.0 to 3.0 mg/kg using 5% DMAC in 0.5% methylcellulose in citrate buffer (pH 3.5). Blood samples were collected at predose and various time points up to 24 hours postdose. All blood samples were collected using EDTA as the anticoagulant and centrifuged to obtain plasma samples. The plasma concentrations of test compounds were determined by LC-MS methods. The measured plasma concentrations were used to calculate PK parameters by standard noncompartmental methods using Phoenix ® WinNonlin software program (version 7.0, Pharsight Corporation). In rats and monkeys, cassette dosing of test compounds were conducted to obtain preliminary PK parameters. In vivo pharmacokinetic experiments with male beagle dogs may be performed under the conditions described above.
• Results from Examples A-E for Compounds of the Examples Compounds of the Examples were assessed in each of the HTRF PD-1/PD-L1 binding assay (Example A), SHP assay (Example B), NFAT assay (Example C), and whole blood internalization assay, 24 hour (Example D).
• Results for the compounds of Examples 1, 2, and 9 are shown in Table 1.
• Results for the compounds of Examples 3, 4, 7, and 14 are shown in Table 2.
• Results for the compounds of Examples 5 and 6 are shown in Table 3.
• Results for the compounds of Examples 8, 10, 11, and 13 are shown in Table 4.
• Results for the compounds of Examples 12, 15, and 16 are shown in Table 5. “nt” is not tested.
• Table 1 Table 2 Table 3 Table 4 Table 5 Certain compounds of the Examples were also assessed for pharmacokinetics in rat or monkey under conditions shown in Example E (see Tables 6 and 7).
• Tables 6 and 7 show the apparent volume of distribution at steady state (V ss ), apparent total body clearance of the drug from plasma (Cl), elimination half-life (t 1/2 ), maximum (peak) plasma concentration (C max ), area under the plasma concentration-time curve from time zero to infinity (AUC 0-inf ), and bioavailability (F).
• V ss apparent total body clearance of the drug from plasma
• C max elimination half-life
• C max maximum (peak) plasma concentration
• AUC 0-inf area under the plasma concentration-time curve from time zero to infinity
• bioavailability F.
• results are from cassette dosing studies: Vss, Cl, and t 1/2 are based on 1.0 mg/kg IV and C max , AUC 0-inf , and F are based on 3.0 mg/kg PO.
• No.20180177870 (‘870) were also tested in the assays of Examples A, B, C, and D (see Table A3; Example numbers refer to the ‘870 disclosure). Certain of the compounds were also assessed for pharmacokinetics in rat (PO at 3.0 mg/kg) under conditions shown in Example E (see Table A4). In Table A4, results are from cassette dosing studies: Vss, Cl, and t1/2 are based on 1.0 mg/kg IV and Cmax, AUC0-inf, and F are based on 3.0 mg/kg PO. Compounds from U.S. Patent Publ.
• No.20180179197 (‘197) were also tested in the assays of Examples A, B, C, and D (see Table A5; Example numbers refer to the ‘197 disclosure). Certain of the compounds were also assessed for pharmacokinetics in rat (PO at 3.0 mg/kg) under conditions shown in Example E (see Table A6). In Table A6, results are from cassette dosing studies: Vss, Cl, and t1/2 are based on 1.0 mg/kg IV and Cmax, AUC0-inf, and F are based on 3.0 mg/kg PO. The disclosures of U.S. Patent Publ. Nos.20180179202, 20180177870, and 20180179197 are incorporated herein by reference in their entireties.

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