JP6838969B2 - 個々の細胞または細胞集団由来の核酸の分析方法 - Google Patents
個々の細胞または細胞集団由来の核酸の分析方法 Download PDFInfo
- Publication number
- JP6838969B2 JP6838969B2 JP2016575111A JP2016575111A JP6838969B2 JP 6838969 B2 JP6838969 B2 JP 6838969B2 JP 2016575111 A JP2016575111 A JP 2016575111A JP 2016575111 A JP2016575111 A JP 2016575111A JP 6838969 B2 JP6838969 B2 JP 6838969B2
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- oligonucleotide
- cells
- nucleic acid
- oligonucleotides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims description 273
- 238000000034 method Methods 0.000 title claims description 241
- 108020004707 nucleic acids Proteins 0.000 title claims description 156
- 102000039446 nucleic acids Human genes 0.000 title claims description 156
- 210000004027 cell Anatomy 0.000 claims description 576
- 108091034117 Oligonucleotide Proteins 0.000 claims description 348
- 239000011324 bead Substances 0.000 claims description 181
- 230000027455 binding Effects 0.000 claims description 128
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 121
- 238000012163 sequencing technique Methods 0.000 claims description 88
- 238000010839 reverse transcription Methods 0.000 claims description 45
- 230000003321 amplification Effects 0.000 claims description 44
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 44
- 230000000295 complement effect Effects 0.000 claims description 41
- 238000006243 chemical reaction Methods 0.000 claims description 31
- 238000003752 polymerase chain reaction Methods 0.000 claims description 29
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 28
- 239000000126 substance Substances 0.000 claims description 12
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims description 7
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims description 7
- 102000018697 Membrane Proteins Human genes 0.000 claims description 5
- 108010052285 Membrane Proteins Proteins 0.000 claims description 5
- 230000003993 interaction Effects 0.000 claims description 4
- 229920002477 rna polymer Polymers 0.000 description 93
- 108020004999 messenger RNA Proteins 0.000 description 91
- 238000004458 analytical method Methods 0.000 description 78
- 239000002299 complementary DNA Substances 0.000 description 76
- 239000012634 fragment Substances 0.000 description 63
- 239000000523 sample Substances 0.000 description 58
- 239000000839 emulsion Substances 0.000 description 51
- 239000012530 fluid Substances 0.000 description 49
- 239000003153 chemical reaction reagent Substances 0.000 description 43
- 125000003729 nucleotide group Chemical group 0.000 description 38
- 239000002773 nucleotide Substances 0.000 description 37
- 230000037452 priming Effects 0.000 description 34
- 238000012512 characterization method Methods 0.000 description 32
- 230000008569 process Effects 0.000 description 30
- 108020004414 DNA Proteins 0.000 description 28
- 239000000203 mixture Substances 0.000 description 26
- 239000003638 chemical reducing agent Substances 0.000 description 22
- 102100034343 Integrase Human genes 0.000 description 20
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 20
- 230000015654 memory Effects 0.000 description 18
- 239000003094 microcapsule Substances 0.000 description 18
- 238000012545 processing Methods 0.000 description 18
- 238000003860 storage Methods 0.000 description 18
- 230000002441 reversible effect Effects 0.000 description 16
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 14
- 230000001413 cellular effect Effects 0.000 description 14
- 230000000694 effects Effects 0.000 description 13
- 206010028980 Neoplasm Diseases 0.000 description 12
- 229920000642 polymer Polymers 0.000 description 12
- 238000013518 transcription Methods 0.000 description 12
- 230000035897 transcription Effects 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 11
- 229940088598 enzyme Drugs 0.000 description 11
- 239000003446 ligand Substances 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 11
- 230000011218 segmentation Effects 0.000 description 11
- 230000009471 action Effects 0.000 description 10
- 201000011510 cancer Diseases 0.000 description 10
- 238000005538 encapsulation Methods 0.000 description 10
- 230000002934 lysing effect Effects 0.000 description 10
- 239000003795 chemical substances by application Substances 0.000 description 9
- 238000010586 diagram Methods 0.000 description 9
- 238000000338 in vitro Methods 0.000 description 9
- -1 labiase Proteins 0.000 description 9
- 239000002243 precursor Substances 0.000 description 9
- 102000004169 proteins and genes Human genes 0.000 description 9
- 239000007790 solid phase Substances 0.000 description 9
- 210000001519 tissue Anatomy 0.000 description 9
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 8
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 description 8
- 108091092328 cellular RNA Proteins 0.000 description 8
- 230000010076 replication Effects 0.000 description 8
- 238000010008 shearing Methods 0.000 description 8
- 238000003559 RNA-seq method Methods 0.000 description 7
- 230000008901 benefit Effects 0.000 description 7
- 229960002685 biotin Drugs 0.000 description 7
- 235000020958 biotin Nutrition 0.000 description 7
- 239000011616 biotin Substances 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 230000001605 fetal effect Effects 0.000 description 7
- 239000011159 matrix material Substances 0.000 description 7
- 244000005700 microbiome Species 0.000 description 7
- 229920002401 polyacrylamide Polymers 0.000 description 7
- 125000006853 reporter group Chemical group 0.000 description 7
- 239000007787 solid Substances 0.000 description 7
- 238000004891 communication Methods 0.000 description 6
- 238000003745 diagnosis Methods 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 125000005647 linker group Chemical group 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 230000004048 modification Effects 0.000 description 6
- 238000012986 modification Methods 0.000 description 6
- 230000035772 mutation Effects 0.000 description 6
- 239000003921 oil Substances 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 238000011002 quantification Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000000638 solvent extraction Methods 0.000 description 6
- 241000894007 species Species 0.000 description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
- 108010042407 Endonucleases Proteins 0.000 description 5
- 102000004533 Endonucleases Human genes 0.000 description 5
- 108010090804 Streptavidin Proteins 0.000 description 5
- 238000013459 approach Methods 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 230000007613 environmental effect Effects 0.000 description 5
- 239000000499 gel Substances 0.000 description 5
- 230000014509 gene expression Effects 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 230000036961 partial effect Effects 0.000 description 5
- 108010001857 Cell Surface Receptors Proteins 0.000 description 4
- 238000001712 DNA sequencing Methods 0.000 description 4
- 108060002716 Exonuclease Proteins 0.000 description 4
- 239000007900 aqueous suspension Substances 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 102000013165 exonuclease Human genes 0.000 description 4
- 238000013467 fragmentation Methods 0.000 description 4
- 238000006062 fragmentation reaction Methods 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 239000011541 reaction mixture Substances 0.000 description 4
- 102000005962 receptors Human genes 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 102000000844 Cell Surface Receptors Human genes 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 108010083644 Ribonucleases Proteins 0.000 description 3
- 102000006382 Ribonucleases Human genes 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 230000006287 biotinylation Effects 0.000 description 3
- 238000007413 biotinylation Methods 0.000 description 3
- 108091092356 cellular DNA Proteins 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000013500 data storage Methods 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000017 hydrogel Substances 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 230000001320 lysogenic effect Effects 0.000 description 3
- 238000007885 magnetic separation Methods 0.000 description 3
- UNEXJVCWJSHFNN-UHFFFAOYSA-N n,n,n',n'-tetraethylmethanediamine Chemical compound CCN(CC)CN(CC)CC UNEXJVCWJSHFNN-UHFFFAOYSA-N 0.000 description 3
- 210000002569 neuron Anatomy 0.000 description 3
- 238000007481 next generation sequencing Methods 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 108020003175 receptors Proteins 0.000 description 3
- 108020004418 ribosomal RNA Proteins 0.000 description 3
- 239000004065 semiconductor Substances 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical group NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- 108010053770 Deoxyribonucleases Proteins 0.000 description 2
- 102000016911 Deoxyribonucleases Human genes 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 102100030569 Nuclear receptor corepressor 2 Human genes 0.000 description 2
- 101710153660 Nuclear receptor corepressor 2 Proteins 0.000 description 2
- 101710086015 RNA ligase Proteins 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 101710137500 T7 RNA polymerase Proteins 0.000 description 2
- 239000013504 Triton X-100 Substances 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000012190 activator Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 239000008364 bulk solution Substances 0.000 description 2
- 230000015861 cell surface binding Effects 0.000 description 2
- 210000003850 cellular structure Anatomy 0.000 description 2
- 239000002738 chelating agent Substances 0.000 description 2
- 210000003040 circulating cell Anatomy 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000004581 coalescence Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 239000003431 cross linking reagent Substances 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 239000000539 dimer Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000004374 forensic analysis Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000003999 initiator Substances 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 230000008774 maternal effect Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- DJVKJGIZQFBFGS-UHFFFAOYSA-N n-[2-[2-(prop-2-enoylamino)ethyldisulfanyl]ethyl]prop-2-enamide Chemical compound C=CC(=O)NCCSSCCNC(=O)C=C DJVKJGIZQFBFGS-UHFFFAOYSA-N 0.000 description 2
- 238000003203 nucleic acid sequencing method Methods 0.000 description 2
- 239000002777 nucleoside Substances 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 230000002085 persistent effect Effects 0.000 description 2
- 239000002861 polymer material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000009781 safety test method Methods 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 231100000041 toxicology testing Toxicity 0.000 description 2
- 235000011178 triphosphate Nutrition 0.000 description 2
- 239000001226 triphosphate Substances 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 101710104316 Cell surface-binding protein Proteins 0.000 description 1
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
- 201000010374 Down Syndrome Diseases 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- LLQPHQFNMLZJMP-UHFFFAOYSA-N Fentrazamide Chemical compound N1=NN(C=2C(=CC=CC=2)Cl)C(=O)N1C(=O)N(CC)C1CCCCC1 LLQPHQFNMLZJMP-UHFFFAOYSA-N 0.000 description 1
- 239000004606 Fillers/Extenders Substances 0.000 description 1
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 208000002250 Hematologic Neoplasms Diseases 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 108090000988 Lysostaphin Proteins 0.000 description 1
- 108010053229 Lysyl endopeptidase Proteins 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 208000025966 Neurological disease Diseases 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 101710160107 Outer membrane protein A Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 101100043635 Solanum tuberosum SS2 gene Proteins 0.000 description 1
- 102000008579 Transposases Human genes 0.000 description 1
- 108010020764 Transposases Proteins 0.000 description 1
- 206010044686 Trisomy 13 Diseases 0.000 description 1
- 208000006284 Trisomy 13 Syndrome Diseases 0.000 description 1
- 206010044688 Trisomy 21 Diseases 0.000 description 1
- 108010064978 Type II Site-Specific Deoxyribonucleases Proteins 0.000 description 1
- 108010067022 Type III Site-Specific Deoxyribonucleases Proteins 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 238000002669 amniocentesis Methods 0.000 description 1
- 208000036878 aneuploidy Diseases 0.000 description 1
- 231100001075 aneuploidy Toxicity 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229920006037 cross link polymer Polymers 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 238000003891 environmental analysis Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 230000009969 flowable effect Effects 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 102000054766 genetic haplotypes Human genes 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000005017 glioblastoma Diseases 0.000 description 1
- 210000005003 heart tissue Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000008241 heterogeneous mixture Substances 0.000 description 1
- 210000003701 histiocyte Anatomy 0.000 description 1
- 244000005702 human microbiome Species 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 210000003000 inclusion body Anatomy 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000002563 ionic surfactant Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000009191 jumping Effects 0.000 description 1
- 108010074304 kitalase Proteins 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 235000019689 luncheon sausage Nutrition 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 108010092948 oligonucleotidase Proteins 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- 238000000016 photochemical curing Methods 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000012704 polymeric precursor Substances 0.000 description 1
- 239000003505 polymerization initiator Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 238000011176 pooling Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011112 process operation Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000012175 pyrosequencing Methods 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000005464 sample preparation method Methods 0.000 description 1
- 238000005204 segregation Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000010454 slate Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000011222 transcriptome analysis Methods 0.000 description 1
- 239000013638 trimer Substances 0.000 description 1
- 206010053884 trisomy 18 Diseases 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 239000007762 w/o emulsion Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B20/00—Methods specially adapted for identifying library members
- C40B20/04—Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
- C40B50/16—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support involving encoding steps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/149—Sequential reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Structural Engineering (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021020743A JP7329552B2 (ja) | 2014-06-26 | 2021-02-12 | 個々の細胞または細胞集団由来の核酸の分析方法 |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462017558P | 2014-06-26 | 2014-06-26 | |
| US62/017,558 | 2014-06-26 | ||
| US201462061567P | 2014-10-08 | 2014-10-08 | |
| US62/061,567 | 2014-10-08 | ||
| PCT/US2015/038178 WO2015200893A2 (en) | 2014-06-26 | 2015-06-26 | Methods of analyzing nucleic acids from individual cells or cell populations |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021020743A Division JP7329552B2 (ja) | 2014-06-26 | 2021-02-12 | 個々の細胞または細胞集団由来の核酸の分析方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017522867A JP2017522867A (ja) | 2017-08-17 |
| JP2017522867A5 JP2017522867A5 (cg-RX-API-DMAC7.html) | 2018-08-02 |
| JP6838969B2 true JP6838969B2 (ja) | 2021-03-03 |
Family
ID=54929862
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016575111A Active JP6838969B2 (ja) | 2014-06-26 | 2015-06-26 | 個々の細胞または細胞集団由来の核酸の分析方法 |
| JP2021020743A Active JP7329552B2 (ja) | 2014-06-26 | 2021-02-12 | 個々の細胞または細胞集団由来の核酸の分析方法 |
| JP2023077527A Pending JP2023099197A (ja) | 2014-06-26 | 2023-05-09 | 個々の細胞または細胞集団由来の核酸の分析方法 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021020743A Active JP7329552B2 (ja) | 2014-06-26 | 2021-02-12 | 個々の細胞または細胞集団由来の核酸の分析方法 |
| JP2023077527A Pending JP2023099197A (ja) | 2014-06-26 | 2023-05-09 | 個々の細胞または細胞集団由来の核酸の分析方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US20150376609A1 (cg-RX-API-DMAC7.html) |
| EP (4) | EP4574974A3 (cg-RX-API-DMAC7.html) |
| JP (3) | JP6838969B2 (cg-RX-API-DMAC7.html) |
| KR (2) | KR102531677B1 (cg-RX-API-DMAC7.html) |
| CN (3) | CN106795553B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2015279548B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2953374A1 (cg-RX-API-DMAC7.html) |
| IL (1) | IL249617A0 (cg-RX-API-DMAC7.html) |
| MX (1) | MX2016016902A (cg-RX-API-DMAC7.html) |
| WO (1) | WO2015200893A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (339)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| CA2636855C (en) | 2006-01-11 | 2016-09-27 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
| ATE540750T1 (de) | 2006-05-11 | 2012-01-15 | Raindance Technologies Inc | Mikrofluidische vorrichtung und verfahren |
| US9562837B2 (en) | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
| WO2008097559A2 (en) | 2007-02-06 | 2008-08-14 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
| US8592221B2 (en) | 2007-04-19 | 2013-11-26 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
| US12038438B2 (en) | 2008-07-18 | 2024-07-16 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| EP2315629B1 (en) | 2008-07-18 | 2021-12-15 | Bio-Rad Laboratories, Inc. | Droplet libraries |
| EP3415235B1 (en) | 2009-03-23 | 2025-11-12 | Bio-Rad Laboratories, Inc. | Manipulation of microfluidic droplets |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
| CA2789425C (en) | 2010-02-12 | 2020-04-28 | Raindance Technologies, Inc. | Digital analyte analysis with polymerase error correction |
| RS54482B1 (sr) | 2010-04-05 | 2016-06-30 | Prognosys Biosciences, Inc. | Biološka testiranja sa prostornim kodiranjem |
| WO2012045012A2 (en) | 2010-09-30 | 2012-04-05 | Raindance Technologies, Inc. | Sandwich assays in droplets |
| EP3859011A1 (en) | 2011-02-11 | 2021-08-04 | Bio-Rad Laboratories, Inc. | Methods for forming mixed droplets |
| EP3736281A1 (en) | 2011-02-18 | 2020-11-11 | Bio-Rad Laboratories, Inc. | Compositions and methods for molecular labeling |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| DE202012013668U1 (de) | 2011-06-02 | 2019-04-18 | Raindance Technologies, Inc. | Enzymquantifizierung |
| US11435358B2 (en) | 2011-06-23 | 2022-09-06 | Board Of Regents, The University Of Texas System | Single molecule peptide sequencing |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
| US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
| ES2797448T3 (es) | 2011-08-01 | 2020-12-02 | Bio Rad Laboratories | Sistema de captura de células |
| US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
| US10941396B2 (en) | 2012-02-27 | 2021-03-09 | Becton, Dickinson And Company | Compositions and kits for molecular counting |
| US10161007B2 (en) | 2012-08-13 | 2018-12-25 | The Regents Of The University Of California | Methods and systems for detecting biological components |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| BR112015003354A8 (pt) | 2012-08-14 | 2018-01-16 | 10X Genomics Inc | métodos e composições de microcápsula |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP3567116A1 (en) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9752181B2 (en) | 2013-01-26 | 2017-09-05 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
| CA2900481A1 (en) | 2013-02-08 | 2014-08-14 | 10X Genomics, Inc. | Polynucleotide barcode generation |
| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
| US9856535B2 (en) | 2013-05-31 | 2018-01-02 | Denovo Sciences, Inc. | System for isolating cells |
| US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
| LT3013983T (lt) | 2013-06-25 | 2023-05-10 | Prognosys Biosciences, Inc. | Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį |
| EP3039158B1 (en) | 2013-08-28 | 2018-11-14 | Cellular Research, Inc. | Massively parallel single cell analysis |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
| EP3055676A1 (en) | 2013-10-07 | 2016-08-17 | Cellular Research, Inc. | Methods and systems for digitally counting features on arrays |
| US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
| US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
| KR102596508B1 (ko) | 2014-04-10 | 2023-10-30 | 10엑스 제노믹스, 인크. | 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용 |
| WO2015200541A1 (en) | 2014-06-24 | 2015-12-30 | Bio-Rad Laboratories, Inc. | Digital pcr barcoding |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| AU2015279546B2 (en) | 2014-06-26 | 2021-04-08 | 10X Genomics, Inc. | Processes and systems for nucleic acid sequence assembly |
| KR20170026383A (ko) | 2014-06-26 | 2017-03-08 | 10엑스 제노믹스, 인크. | 핵산 서열의 분석 |
| EP4574974A3 (en) | 2014-06-26 | 2025-10-08 | 10x Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| US20160122817A1 (en) | 2014-10-29 | 2016-05-05 | 10X Genomics, Inc. | Methods and compositions for targeted nucleic acid sequencing |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| SG11201705615UA (en) | 2015-01-12 | 2017-08-30 | 10X Genomics Inc | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
| EP3245605B1 (en) | 2015-01-13 | 2022-04-20 | 10X Genomics, Inc. | Systems and methods for visualizing structural variation and phasing information |
| CA2974306A1 (en) * | 2015-02-04 | 2016-08-11 | The Regents Of The University Of California | Sequencing of nucleic acids via barcoding in discrete entities |
| JP2018513445A (ja) | 2015-02-09 | 2018-05-24 | 10エックス ゲノミクス,インコーポレイテッド | 構造変異の特定及びバリアントコールデータを用いたフェージングのためのシステム及び方法 |
| WO2016134078A1 (en) | 2015-02-19 | 2016-08-25 | Becton, Dickinson And Company | High-throughput single-cell analysis combining proteomic and genomic information |
| US10697000B2 (en) | 2015-02-24 | 2020-06-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| CN107532202A (zh) | 2015-02-24 | 2018-01-02 | 10X 基因组学有限公司 | 用于靶向核酸序列覆盖的方法 |
| US9727810B2 (en) | 2015-02-27 | 2017-08-08 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| JP7508191B2 (ja) | 2015-03-30 | 2024-07-01 | ベクトン・ディキンソン・アンド・カンパニー | コンビナトリアルバーコーディングのための方法および組成物 |
| HK1250245A1 (zh) | 2015-04-10 | 2018-12-07 | Spatial Transcriptomics Ab | 生物样本的空间区别、多重核酸分析 |
| WO2016172373A1 (en) | 2015-04-23 | 2016-10-27 | Cellular Research, Inc. | Methods and compositions for whole transcriptome amplification |
| WO2016196229A1 (en) | 2015-06-01 | 2016-12-08 | Cellular Research, Inc. | Methods for rna quantification |
| US9938558B2 (en) | 2015-06-25 | 2018-04-10 | Ascus Biosciences, Inc. | Methods, apparatuses, and systems for analyzing microorganism strains from complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and selecting and synthesizing microbial ensembles based thereon |
| AU2016282996A1 (en) | 2015-06-25 | 2018-01-18 | Ascus Biosciences, Inc. | Methods, apparatuses, and systems for analyzing microorganism strains from complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and selecting and synthesizing microbial ensembles based thereon |
| US10851399B2 (en) | 2015-06-25 | 2020-12-01 | Native Microbials, Inc. | Methods, apparatuses, and systems for microorganism strain analysis of complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and selecting and synthesizing microbial ensembles based thereon |
| KR102395450B1 (ko) | 2015-09-11 | 2022-05-09 | 셀룰러 리서치, 인크. | 핵산 라이브러리 정규화를 위한 방법 및 조성물 |
| WO2017079593A1 (en) * | 2015-11-04 | 2017-05-11 | Atreca, Inc. | Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells |
| US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
| EP4144861B1 (en) | 2015-12-04 | 2024-09-11 | 10X Genomics, Inc. | Methods and compositions for nucleic acid analysis |
| EP3397764A4 (en) | 2015-12-30 | 2019-05-22 | Bio-Rad Laboratories, Inc. | DIGITAL PROTEIN QUANTIFICATION |
| US9993507B2 (en) | 2016-01-07 | 2018-06-12 | Ascus Biosciences, Inc. | Methods of improving milk production and compositional characteristics |
| US12083512B2 (en) * | 2016-01-14 | 2024-09-10 | European Molecular Biology Laboratory | Microfluidic analysis of ligand induced cell expression |
| JP6735348B2 (ja) | 2016-02-11 | 2020-08-05 | 10エックス ジェノミクス, インコーポレイテッド | 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体 |
| US20170260584A1 (en) * | 2016-02-11 | 2017-09-14 | 10X Genomics, Inc. | Cell population analysis using single nucleotide polymorphisms from single cell transcriptomes |
| US20190127789A1 (en) * | 2016-04-19 | 2019-05-02 | President And Fellows Of Harvard College | Immobilization-based systems and methods for genetic analysis and other applications |
| EP3452614B1 (en) | 2016-05-02 | 2023-06-28 | Becton, Dickinson and Company | Accurate molecular barcoding |
| KR102741603B1 (ko) | 2016-05-02 | 2024-12-16 | 엔코디아, 인코포레이티드 | 암호화 핵산을 사용한 거대분자 분석 |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| CN109154021A (zh) * | 2016-05-25 | 2019-01-04 | 生物辐射实验室股份有限公司 | 数字邻近试验 |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| EP3465502B1 (en) | 2016-05-26 | 2024-04-10 | Becton, Dickinson and Company | Molecular label counting adjustment methods |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US20190329209A1 (en) * | 2016-06-14 | 2019-10-31 | Mission Bio, Inc. | Methods and compositions for emulsification of solid supports in deformable beads |
| EP3686287A1 (en) | 2016-06-28 | 2020-07-29 | Hifibio | Method for transcriptome analysis of single cells |
| EP3488239A1 (en) | 2016-07-22 | 2019-05-29 | Verily Life Sciences LLC | Quantitative massively parallel proteomics |
| EP3516400B1 (en) | 2016-09-26 | 2023-08-16 | Becton, Dickinson and Company | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| CN114875125A (zh) * | 2016-10-19 | 2022-08-09 | 10X基因组学有限公司 | 用于条形码化单个细胞或细胞群的核酸分子的方法和系统 |
| ES2980967T3 (es) | 2016-11-08 | 2024-10-03 | Becton Dickinson Co | Métodos para la clasificación de perfiles de expresión |
| KR102790039B1 (ko) | 2016-11-08 | 2025-04-04 | 벡톤 디킨슨 앤드 컴퍼니 | 세포 표지 분류 방법 |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US20190177800A1 (en) * | 2017-12-08 | 2019-06-13 | 10X Genomics, Inc. | Methods and compositions for labeling cells |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| GB201622222D0 (en) * | 2016-12-23 | 2017-02-08 | Cs Genetics Ltd | Reagents and methods for molecular barcoding of nucleic acids of single cells |
| CA3048246A1 (en) | 2016-12-28 | 2018-07-05 | Ascus Biosciences, Inc. | Methods, apparatuses, and systems for analyzing complete microorganism strains in complex heterogeneous communities, determining functional relationships and interactions thereof, and identifying and synthesizing bioreactive modificators based thereon |
| AU2017388535A1 (en) | 2016-12-28 | 2019-07-25 | Native Microbials, Inc. | Methods, apparatuses, and systems for microorganism strain analysis of complex heterogeneous communities with tracer analytics, determination of functional relationships and interactions thereof, and synthesis of microbial ensembles including dosed microbial ensembles and inoculative microbial ensembles |
| JP7104048B2 (ja) | 2017-01-13 | 2022-07-20 | セルラー リサーチ, インコーポレイテッド | 流体チャネルの親水性コーティング |
| WO2018134907A1 (ja) * | 2017-01-18 | 2018-07-26 | 株式会社日立ハイテクノロジーズ | 同一細胞複数生体分子抽出装置および方法 |
| CN110291207A (zh) | 2017-01-27 | 2019-09-27 | 豪夫迈·罗氏有限公司 | 用于长程测序的条形码化dna |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| EP4310183B1 (en) | 2017-01-30 | 2025-07-09 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| US11319583B2 (en) | 2017-02-01 | 2022-05-03 | Becton, Dickinson And Company | Selective amplification using blocking oligonucleotides |
| DK3583214T5 (da) | 2017-02-02 | 2024-09-02 | New York Genome Center Inc | Fremgangsmåder og sammensætninger til identificering eller kvantificering af mål i en biologisk prøve |
| US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| US11954614B2 (en) | 2017-02-08 | 2024-04-09 | 10X Genomics, Inc. | Systems and methods for visualizing a pattern in a dataset |
| US10347365B2 (en) | 2017-02-08 | 2019-07-09 | 10X Genomics, Inc. | Systems and methods for visualizing a pattern in a dataset |
| US12054764B2 (en) * | 2017-04-14 | 2024-08-06 | The Broad Institute, Inc. | High-throughput screens for exploring biological functions of microscale biological systems |
| CN110637084A (zh) | 2017-04-26 | 2019-12-31 | 10X基因组学有限公司 | Mmlv逆转录酶变体 |
| CA3057692A1 (en) | 2017-04-28 | 2018-11-01 | Ascus Biosciences, Inc. | Methods for supporting grain intensive and/or energy intensive diets in ruminants by administration of a synthetic bioensemble of microbes or purified strains thereof |
| WO2018203141A1 (en) * | 2017-05-05 | 2018-11-08 | Scipio Bioscience | Methods for trapping and barcoding discrete biological units in hydrogel |
| CN110945139B (zh) | 2017-05-18 | 2023-09-05 | 10X基因组学有限公司 | 用于分选液滴和珠的方法和系统 |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| CN110870018B (zh) | 2017-05-19 | 2024-11-22 | 10X基因组学有限公司 | 用于分析数据集的系统和方法 |
| CN109526228B (zh) * | 2017-05-26 | 2022-11-25 | 10X基因组学有限公司 | 转座酶可接近性染色质的单细胞分析 |
| MA49352A (fr) * | 2017-05-26 | 2020-04-08 | Abvitro Llc | Séquençage de bibliothèque de polynucléotides à haut rendement et analyse de transcriptome |
| US20180340169A1 (en) | 2017-05-26 | 2018-11-29 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| WO2018218226A1 (en) * | 2017-05-26 | 2018-11-29 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN111406114A (zh) * | 2017-05-29 | 2020-07-10 | 哈佛学院董事及会员团体 | 扩增单个细胞转录组的方法 |
| JP7536450B2 (ja) | 2017-06-05 | 2024-08-20 | ベクトン・ディキンソン・アンド・カンパニー | 単一細胞用のサンプルインデックス付加 |
| WO2018236615A1 (en) | 2017-06-20 | 2018-12-27 | 10X Genomics, Inc. | Methods and systems for improved droplet stabilization |
| KR102694654B1 (ko) * | 2017-08-01 | 2024-08-12 | 일루미나, 인코포레이티드 | 뉴클레오티드 서열분석용 하이드로겔 비드 |
| CN107222286B (zh) * | 2017-08-09 | 2018-03-13 | 罗普特(厦门)科技集团有限公司 | 一种网络定向屏蔽的方法 |
| US10357771B2 (en) | 2017-08-22 | 2019-07-23 | 10X Genomics, Inc. | Method of producing emulsions |
| WO2019046307A1 (en) | 2017-08-29 | 2019-03-07 | Celsee Diagnostics, Inc. | SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS |
| CN111132765B (zh) * | 2017-09-19 | 2022-05-13 | 高保真生物技术有限公司 | 微流体系统中的颗粒分选 |
| US10837047B2 (en) | 2017-10-04 | 2020-11-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| US10590244B2 (en) | 2017-10-04 | 2020-03-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| EP3691703A1 (en) | 2017-10-04 | 2020-08-12 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| US10501739B2 (en) * | 2017-10-18 | 2019-12-10 | Mission Bio, Inc. | Method, systems and apparatus for single cell analysis |
| WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS |
| WO2019084328A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS FOR PREPARING NUCLEIC ACID MOLECULES |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| CN114525273B (zh) | 2017-10-27 | 2025-01-28 | 10X基因组学有限公司 | 用于样品制备和分析的方法和系统 |
| WO2019089836A1 (en) | 2017-10-31 | 2019-05-09 | Encodia, Inc. | Kits for analysis using nucleic acid encoding and/or label |
| EP3704246A4 (en) | 2017-10-31 | 2021-08-11 | Encodia, Inc. | PROCESSES AND KITS USING CODING AND / OR MARKING BY NUCLEIC ACIDS |
| WO2019099751A1 (en) | 2017-11-15 | 2019-05-23 | 10X Genomics, Inc. | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| WO2019099908A1 (en) | 2017-11-17 | 2019-05-23 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| WO2019108851A1 (en) | 2017-11-30 | 2019-06-06 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation and analysis |
| WO2019113235A1 (en) | 2017-12-06 | 2019-06-13 | 10X Genomics, Inc. | Methods and systems for processing nucleic acid molecules |
| EP3568494B1 (en) | 2017-12-08 | 2021-05-05 | 10X Genomics, Inc. | Methods and compositions for labeling cells |
| WO2019118355A1 (en) | 2017-12-12 | 2019-06-20 | 10X Genomics, Inc. | Systems and methods for single cell processing |
| CN111492068B (zh) | 2017-12-19 | 2025-03-21 | 贝克顿迪金森公司 | 与寡核苷酸相关联的颗粒 |
| CN118547046A (zh) | 2017-12-22 | 2024-08-27 | 10X基因组学有限公司 | 用于处理来自一个或多个细胞的核酸分子的系统和方法 |
| WO2019148042A1 (en) | 2018-01-26 | 2019-08-01 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| EP4299755A3 (en) | 2018-02-05 | 2024-04-10 | The Board of Trustees of the Leland Stanford Junior University | Systems and methods for multiplexed measurements in single and ensemble cells |
| CN112005115A (zh) | 2018-02-12 | 2020-11-27 | 10X基因组学有限公司 | 表征来自单个细胞或细胞群体的多种分析物的方法 |
| US11180752B2 (en) | 2018-02-13 | 2021-11-23 | Illumina, Inc. | DNA sequencing using hydrogel beads |
| SG11202008080RA (en) | 2018-02-22 | 2020-09-29 | 10X Genomics Inc | Ligation mediated analysis of nucleic acids |
| US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
| WO2019169028A1 (en) | 2018-02-28 | 2019-09-06 | 10X Genomics, Inc. | Transcriptome sequencing through random ligation |
| WO2019191321A1 (en) | 2018-03-28 | 2019-10-03 | 10X Genomics, Inc. | Nucleic acid enrichment within partitions |
| SG11202009889VA (en) | 2018-04-06 | 2020-11-27 | 10X Genomics Inc | Systems and methods for quality control in single cell processing |
| KR20240052875A (ko) | 2018-04-20 | 2024-04-23 | 일루미나, 인코포레이티드 | 단일 세포를 캡슐화하는 방법, 캡슐화된 세포 및 이의 용도 |
| CN118910215A (zh) | 2018-05-03 | 2024-11-08 | 贝克顿迪金森公司 | 在相对的转录物末端进行分子条形码化 |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| US11039856B2 (en) | 2018-05-10 | 2021-06-22 | Verily Life Sciences Llc | Surgical laparoscopic ports for implanting medical systems |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| US11703427B2 (en) | 2018-06-25 | 2023-07-18 | 10X Genomics, Inc. | Methods and systems for cell and bead processing |
| GB201810571D0 (en) * | 2018-06-27 | 2018-08-15 | Cs Genetics Ltd | Reagents and methods for the analysis of circulating microparticles |
| WO2020009998A1 (en) | 2018-07-03 | 2020-01-09 | Chan Zuckerberg Biohub, Inc. | Method to perform high-throughput single cell genomic and phenotypic analyses |
| AU2019301750A1 (en) | 2018-07-12 | 2021-01-28 | Board Of Regents, The University Of Texas System | Molecular neighborhood detection by oligonucleotides |
| EP3821035A4 (en) * | 2018-07-13 | 2022-04-20 | Coral Genomics, Inc. | Methods for analyzing cells |
| US12188014B1 (en) | 2018-07-25 | 2025-01-07 | 10X Genomics, Inc. | Compositions and methods for nucleic acid processing using blocking agents |
| US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| EP3830289A1 (en) | 2018-08-03 | 2021-06-09 | 10X Genomics, Inc. | Methods and systems to minimize barcode exchange |
| WO2020041148A1 (en) | 2018-08-20 | 2020-02-27 | 10X Genomics, Inc. | Methods and systems for detection of protein-dna interactions using proximity ligation |
| US12065688B2 (en) | 2018-08-20 | 2024-08-20 | 10X Genomics, Inc. | Compositions and methods for cellular processing |
| US20210317524A1 (en) | 2018-08-28 | 2021-10-14 | 10X Genomics, Inc. | Resolving spatial arrays |
| US20210332424A1 (en) | 2018-08-28 | 2021-10-28 | 10X Genomics, Inc. | Methods of generating an array |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| US11639517B2 (en) | 2018-10-01 | 2023-05-02 | Becton, Dickinson And Company | Determining 5′ transcript sequences |
| CN113015740A (zh) | 2018-10-05 | 2021-06-22 | 德克萨斯大学系统董事会 | 固相n-末端肽的捕获和释放 |
| JP7528067B2 (ja) * | 2018-10-25 | 2024-08-05 | イルミナ インコーポレイテッド | インデックス及びバーコードを使用してアレイ上のリガンドを識別するための方法及び組成物 |
| US11608524B1 (en) * | 2018-10-25 | 2023-03-21 | Wisconsin Alumni Research Foundation | Methods of analyzing cells |
| EP4656734A2 (en) | 2018-10-26 | 2025-12-03 | Illumina, Inc. | Modulating polymer beads for dna processing |
| US11932849B2 (en) | 2018-11-08 | 2024-03-19 | Becton, Dickinson And Company | Whole transcriptome analysis of single cells using random priming |
| US11636921B2 (en) | 2018-11-27 | 2023-04-25 | 10X Genomics, Inc. | Systems and methods for inferring cell status |
| EP4567127A3 (en) | 2018-12-10 | 2025-09-03 | 10x Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
| US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
| CN113195717A (zh) | 2018-12-13 | 2021-07-30 | 贝克顿迪金森公司 | 单细胞全转录组分析中的选择性延伸 |
| CN111378557B (zh) | 2018-12-26 | 2023-06-06 | 财团法人工业技术研究院 | 用于产生液珠的管状结构及液珠产生方法 |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| CN113874521B (zh) | 2019-01-06 | 2024-11-01 | 10X基因组学有限公司 | 用于富集条形码的方法和系统 |
| US12169198B2 (en) * | 2019-01-08 | 2024-12-17 | 10X Genomics, Inc. | Systems and methods for sample analysis |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| WO2020150356A1 (en) | 2019-01-16 | 2020-07-23 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
| EP4242322B1 (en) | 2019-01-23 | 2024-08-21 | Becton, Dickinson and Company | Oligonucleotides associated with antibodies |
| US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| WO2020168013A1 (en) * | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| WO2020167862A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transfer of reagents between droplets |
| WO2020167866A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transposon loading |
| US12275993B2 (en) | 2019-02-12 | 2025-04-15 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
| US12305239B2 (en) | 2019-02-12 | 2025-05-20 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| GB2582850A (en) * | 2019-02-13 | 2020-10-07 | Biosearch Tech Inc | Molecular barcodes for single cell sequencing, compositions and methods thereof |
| US12071617B2 (en) | 2019-02-14 | 2024-08-27 | Becton, Dickinson And Company | Hybrid targeted and whole transcriptome amplification |
| US11655499B1 (en) | 2019-02-25 | 2023-05-23 | 10X Genomics, Inc. | Detection of sequence elements in nucleic acid molecules |
| CN114174531A (zh) | 2019-02-28 | 2022-03-11 | 10X基因组学有限公司 | 用空间条码化寡核苷酸阵列对生物分析物进行概况分析 |
| US11920183B2 (en) | 2019-03-11 | 2024-03-05 | 10X Genomics, Inc. | Systems and methods for processing optically tagged beads |
| CN114127309A (zh) | 2019-03-15 | 2022-03-01 | 10X基因组学有限公司 | 使用空间阵列进行单细胞测序的方法 |
| EP3887542A1 (en) | 2019-03-22 | 2021-10-06 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| EP3947722A1 (en) | 2019-03-27 | 2022-02-09 | 10X Genomics, Inc. | Systems and methods for processing rna from cells |
| CN113874423B (zh) | 2019-04-03 | 2025-02-14 | 10X基因组学有限公司 | 用于使用改进的聚合物形成珠粒的组合物、方法和系统 |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| WO2020214642A1 (en) | 2019-04-19 | 2020-10-22 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| JP7582676B2 (ja) | 2019-04-30 | 2024-11-13 | エンコディア, インコーポレイテッド | 分析物を調製するための方法および関連キット |
| SG11202112151UA (en) | 2019-05-07 | 2021-12-30 | Bio Rad Laboratories | System and method for automated single cell processing |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| US11365441B2 (en) | 2019-05-22 | 2022-06-21 | Mission Bio, Inc. | Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| CN114302643B (zh) | 2019-06-14 | 2024-02-27 | 伯乐实验室有限公司 | 用于自动化单细胞处理和分析的系统和方法 |
| WO2021003255A1 (en) | 2019-07-01 | 2021-01-07 | Mission Bio | Method and apparatus to normalize quantitative readouts in single-cell experiments |
| CN114096679B (zh) * | 2019-07-11 | 2024-04-09 | 学校法人东京理科大学 | 使用固相载体的核酸扩增方法 |
| US11939622B2 (en) | 2019-07-22 | 2024-03-26 | Becton, Dickinson And Company | Single cell chromatin immunoprecipitation sequencing assay |
| CA3153236A1 (en) * | 2019-09-06 | 2021-03-11 | Cemm-Forschungszentrum Fur Molekulare Medizin Gmbh | Method for sequencing rna oligonucleotides |
| CN114729392A (zh) * | 2019-09-06 | 2022-07-08 | 10X基因组学有限公司 | 用于对细胞和细胞珠粒进行条形码化的系统和方法 |
| US12235262B1 (en) | 2019-09-09 | 2025-02-25 | 10X Genomics, Inc. | Methods and systems for single cell protein analysis |
| MX2021015885A (es) * | 2019-09-20 | 2022-03-22 | Illumina Inc | Métodos y composiciones para identificar ligandos en arreglos usando índices y códigos de barra. |
| CN114761992B (zh) | 2019-10-01 | 2023-08-08 | 10X基因组学有限公司 | 用于识别组织样品中的形态学模式的系统和方法 |
| GB2604481A (en) | 2019-10-10 | 2022-09-07 | 1859 Inc | Methods and systems for microfluidic screening |
| AU2020364147A1 (en) | 2019-10-11 | 2022-05-26 | 10X Genomics, Inc. | Methods for analyte detection and analysis |
| EP4055185A1 (en) | 2019-11-08 | 2022-09-14 | 10X Genomics, Inc. | Spatially-tagged analyte capture agents for analyte multiplexing |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用 |
| EP4058598B1 (en) | 2019-11-13 | 2025-04-23 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| CA3158603A1 (en) | 2019-11-18 | 2021-05-27 | Neil Ira WEISENFELD | Systems and methods for tissue classification |
| US12163189B2 (en) | 2019-11-27 | 2024-12-10 | University Of Washington | Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria |
| EP4461810A3 (en) | 2019-12-11 | 2025-04-09 | 10X Genomics, Inc. | Reverse transcriptase variants |
| EP4081777A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Reversible fixing reagents and methods of use thereof |
| EP3891300B1 (en) | 2019-12-23 | 2023-03-29 | 10X Genomics, Inc. | Methods for spatial analysis using rna-templated ligation |
| US12241890B2 (en) | 2019-12-23 | 2025-03-04 | 10X Genomics, Inc. | Methods for generating barcoded nucleic acid molecules using fixed cells |
| WO2021146207A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and rna |
| JP2023511279A (ja) | 2020-01-13 | 2023-03-17 | フルーエント バイオサイエンシーズ インコーポレイテッド | 単一細胞シーケンシング |
| CA3167719A1 (en) * | 2020-01-13 | 2021-07-22 | Fluent Biosciences Inc. | Methods and systems for single cell gene profiling |
| US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
| ES2993319T3 (en) | 2020-01-29 | 2024-12-27 | Becton Dickinson Co | Barcoded wells for spatial mapping of single cells through sequencing |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12112833B2 (en) | 2020-02-04 | 2024-10-08 | 10X Genomics, Inc. | Systems and methods for index hopping filtering |
| US12449419B1 (en) | 2020-02-12 | 2025-10-21 | 10X Genomics, Inc. | Methods for detecting binding of peptide-MHC monomers to T cells |
| US12421558B2 (en) | 2020-02-13 | 2025-09-23 | 10X Genomics, Inc. | Systems and methods for joint interactive visualization of gene expression and DNA chromatin accessibility |
| AU2021221158A1 (en) | 2020-02-13 | 2022-09-22 | 10X Genomics, Inc. | Methods for characterizing cells using gene expression and chromatin accessibility |
| WO2021168287A1 (en) | 2020-02-21 | 2021-08-26 | 10X Genomics, Inc. | Methods and compositions for integrated in situ spatial assay |
| CN115151810A (zh) | 2020-02-25 | 2022-10-04 | 贝克顿迪金森公司 | 实现使用单细胞样品作为单色补偿对照的双特异性探针 |
| CN115461470A (zh) | 2020-02-28 | 2022-12-09 | 10X基因组学有限公司 | 用于从组织中分离核和细胞的方法 |
| US11504719B2 (en) | 2020-03-12 | 2022-11-22 | Bio-Rad Laboratories, Inc. | System and method for receiving and delivering a fluid for sample processing |
| WO2021188500A1 (en) | 2020-03-16 | 2021-09-23 | Fluent Biosciences Inc. | Multi-omic analysis in monodisperse droplets |
| CA3153296A1 (en) * | 2020-03-24 | 2021-09-30 | Alex Chenchik | Single cell genetic analysis |
| US12428685B2 (en) | 2020-03-24 | 2025-09-30 | Illumina, Inc. | Viral detection using template emulsification |
| WO2021207610A1 (en) | 2020-04-10 | 2021-10-14 | 10X Genomics, Inc. | Cold protease treatment method for preparing biological samples |
| EP4136227A1 (en) * | 2020-04-16 | 2023-02-22 | 10X Genomics, Inc. | Compositions and methods for use with fixed samples |
| WO2021222302A1 (en) | 2020-04-27 | 2021-11-04 | 10X Genomics, Inc. | Methods and systems for increasing cell recovery efficiency |
| WO2021222301A1 (en) | 2020-04-27 | 2021-11-04 | 10X Genomics, Inc. | Methods and systems for analysis and identification of barcode multiplets |
| WO2021226290A1 (en) | 2020-05-05 | 2021-11-11 | 10X Genomics, Inc. | Methods for identification of antigen-binding molecules |
| US11851700B1 (en) | 2020-05-13 | 2023-12-26 | 10X Genomics, Inc. | Methods, kits, and compositions for processing extracellular molecules |
| CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司(Us) | 用于免疫组库谱分析的引物 |
| CN115803445A (zh) | 2020-06-02 | 2023-03-14 | 贝克顿迪金森公司 | 用于5撇基因表达测定的寡核苷酸和珠 |
| US12265079B1 (en) | 2020-06-02 | 2025-04-01 | 10X Genomics, Inc. | Systems and methods for detecting analytes from captured single biological particles |
| WO2021247618A1 (en) | 2020-06-02 | 2021-12-09 | 10X Genomics, Inc. | Enrichment of nucleic acid sequences |
| WO2022006455A1 (en) | 2020-07-02 | 2022-01-06 | 10X Genomics, Inc. | Systems and methods for detection of low-abundance molecular barcodes from a sequencing library |
| US12168801B1 (en) | 2020-07-02 | 2024-12-17 | 10X Genomics, Inc. | Hybrid/capture probe designs for full-length cDNA |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2022015970A1 (en) | 2020-07-15 | 2022-01-20 | Fluent Biosciences Inc. | Tiered ligation oligos |
| WO2022026909A1 (en) | 2020-07-31 | 2022-02-03 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| WO2022035729A1 (en) | 2020-08-10 | 2022-02-17 | Dimensiongen | Devices and methods for multi-dimensional genome analysis |
| EP4217464B1 (en) | 2020-09-23 | 2024-05-22 | 10X Genomics, Inc. | Selective enzymatic gelation |
| US12298314B2 (en) | 2020-10-09 | 2025-05-13 | 10X Genomics, Inc. | Methods and compositions for analyzing target binding of molecules |
| EP4225934B1 (en) | 2020-10-09 | 2024-07-24 | 10X Genomics, Inc. | Methods and compositions for analyzing antigen binding molecules |
| WO2022076914A1 (en) | 2020-10-09 | 2022-04-14 | 10X Genomics, Inc. | Methods and compositions for profiling immune repertoire |
| WO2022081643A2 (en) | 2020-10-13 | 2022-04-21 | 10X Genomics, Inc. | Compositions and methods for generating recombinant antigen binding molecules from single cells |
| US12084715B1 (en) | 2020-11-05 | 2024-09-10 | 10X Genomics, Inc. | Methods and systems for reducing artifactual antisense products |
| US12480158B1 (en) | 2020-11-05 | 2025-11-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP4244379A1 (en) | 2020-11-13 | 2023-09-20 | 10X Genomics, Inc. | Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays |
| WO2022109343A1 (en) | 2020-11-20 | 2022-05-27 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| US20240076723A1 (en) | 2020-12-30 | 2024-03-07 | 10X Genomics, Inc. | Cleavage of capture probes for spatial analysis |
| WO2022150662A1 (en) | 2021-01-08 | 2022-07-14 | 10X Genomics, Inc. | Methods for generating antigen-binding molecules from single cells |
| US12398262B1 (en) | 2021-01-22 | 2025-08-26 | 10X Genomics, Inc. | Triblock copolymer-based cell stabilization and fixation system and methods of use thereof |
| WO2022178304A1 (en) | 2021-02-19 | 2022-08-25 | 10X Genomics, Inc. | High-throughput methods for analyzing and affinity-maturing an antigen-binding molecule |
| WO2022182662A1 (en) | 2021-02-23 | 2022-09-01 | 10X Genomics, Inc. | Compositions and methods for mapping antigen-binding molecule affinity to antigen regions of interest |
| WO2022182672A1 (en) | 2021-02-23 | 2022-09-01 | 10X Genomics, Inc. | Single cell glycan profiling |
| CN120158497A (zh) | 2021-02-23 | 2025-06-17 | 10X基因组学有限公司 | 基于探针的核酸和蛋白质分析 |
| WO2022182664A1 (en) | 2021-02-23 | 2022-09-01 | 10X Genomics, Inc. | A method for epitope binning of novel monoclonal antibodies |
| WO2022182785A1 (en) | 2021-02-23 | 2022-09-01 | 10X Genomics, Inc. | Drug screening methods |
| WO2022221428A1 (en) | 2021-04-14 | 2022-10-20 | 10X Genomics, Inc. | Compositions and methods for single cell analyte detection and analysis |
| CN115011679B (zh) * | 2021-05-12 | 2023-04-11 | 浙江大学 | 一种超高通量单细胞测序方法 |
| WO2022256313A1 (en) | 2021-06-01 | 2022-12-08 | 10X Genomics, Inc. | Validation of a unique molecular identifier associated with a nucleic acid sequence of interest |
| WO2022256345A1 (en) | 2021-06-01 | 2022-12-08 | 10X Genomics, Inc. | Methods and systems for engineering antibodies, and antigen-binding fragments thereof, to have altered characteristics |
| WO2022265965A1 (en) | 2021-06-14 | 2022-12-22 | 10X Genomics, Inc. | Reverse transcriptase variants for improved performance |
| WO2022271908A1 (en) | 2021-06-23 | 2022-12-29 | 10X Genomics, Inc. | Chop-fix method and chopping device for preparing biological samples |
| US20230019117A1 (en) * | 2021-07-15 | 2023-01-19 | Fluent Biosciences Inc. | Decentralized workflows for single cell analysis |
| WO2023009988A1 (en) | 2021-07-26 | 2023-02-02 | 10X Genomics, Inc. | Nucleic acid processing via circularization |
| EP4388123B1 (en) | 2021-08-17 | 2025-06-18 | 10X Genomics, Inc. | Compositions, systems and methods for enzyme optimization |
| WO2023060110A1 (en) | 2021-10-05 | 2023-04-13 | 10X Genomics, Inc. | Methods of immune cell analysis |
| WO2023059646A1 (en) | 2021-10-06 | 2023-04-13 | 10X Genomics, Inc. | Systems and methods for evaluating biological samples |
| US20250147036A1 (en) | 2021-11-10 | 2025-05-08 | 10X Genomics, Inc. | Methods for identification of antigen-binding molecules |
| WO2023107539A1 (en) | 2021-12-10 | 2023-06-15 | Bio-Rad Laboratories, Inc. | Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles |
| EP4448787A1 (en) | 2021-12-13 | 2024-10-23 | Cornell University | Genotyping of targeted loci with single-cell chromatin accessibility |
| WO2023114310A1 (en) | 2021-12-15 | 2023-06-22 | 10X Genomics, Inc. | Methods for improving sensitivity of immune profiling using oligo-tagged antigens |
| WO2023114473A2 (en) | 2021-12-16 | 2023-06-22 | 10X Genomics, Inc. | Recombinant reverse transcriptase variants for improved performance |
| US20250251403A1 (en) | 2022-04-12 | 2025-08-07 | 10X Genomics, Inc. | Compositions and methods for generating and characterizing recombinant antigen binding molecules |
| US20250272996A1 (en) | 2022-04-26 | 2025-08-28 | 10X Genomics, Inc. | Systems and methods for evaluating biological samples |
| EP4272764A1 (en) | 2022-05-03 | 2023-11-08 | Scipio Bioscience | Method of complexing biological units with particles |
| WO2023215612A1 (en) | 2022-05-06 | 2023-11-09 | 10X Genomics, Inc. | Analysis of antigen and antigen receptor interactions |
| WO2023215861A1 (en) | 2022-05-06 | 2023-11-09 | 10X Genomics, Inc. | Reagents for characterizing antigen-binding molecules from immune cells |
| WO2023225294A1 (en) | 2022-05-20 | 2023-11-23 | 10X Genomics, Inc. | Improved major histocompatibility complex molecules |
| WO2023225259A1 (en) | 2022-05-20 | 2023-11-23 | 10X Genomics, Inc. | Compositions and methods for characterizing antigen binding molecules from single cells |
| WO2023225201A1 (en) | 2022-05-20 | 2023-11-23 | 10X Genomics, Inc. | Compositions and methods for characterizing t cell, or t cell-like, receptors from single cells |
| WO2023235570A1 (en) | 2022-06-03 | 2023-12-07 | 10X Genomics, Inc. | Methods and compositions for the identification of antigen binding molecules using lipoparticle-based antigen mapping |
| WO2023235596A1 (en) | 2022-06-03 | 2023-12-07 | 10X Genomics, Inc. | Systems and methods for determining antigen binding specificity of antigen binding molecules |
| WO2023250422A1 (en) | 2022-06-23 | 2023-12-28 | 10X Genomics, Inc. | Compositions and methods for characterizing multispecific antigen binding molecules from single cells |
| WO2024006734A1 (en) | 2022-06-27 | 2024-01-04 | 10X Genomics, Inc. | Methods for preparing and using mhc multimer reagents compositions |
| EP4547858A1 (en) | 2022-06-29 | 2025-05-07 | 10x Genomics, Inc. | Probe-based analysis of nucleic acids and proteins |
| EP4554973A1 (en) | 2022-07-13 | 2025-05-21 | 10X Genomics, Inc. | Methods for characterization of antigen-binding molecules from biological samples |
| WO2024015856A1 (en) | 2022-07-13 | 2024-01-18 | 10X Genomics, Inc. | Compositions and methods for characterizing binding characteristics of antigen binding molecules from single cells |
| WO2024015733A1 (en) | 2022-07-13 | 2024-01-18 | 10X Genomics, Inc. | Improved methods and systems for identification and characterization of antigen-binding molecules from single cells |
| WO2024015378A1 (en) | 2022-07-13 | 2024-01-18 | 10X Genomics, Inc. | Methods and systems for characterizing antigen-binding molecules expressed by immune cells |
| WO2024044703A1 (en) | 2022-08-24 | 2024-02-29 | 10X Genomics, Inc. | Compositions and methods for antigenic epitope mapping in biological samples |
| WO2024050299A1 (en) | 2022-08-29 | 2024-03-07 | 10X Genomics, Inc. | Improved methods and compositions for characterization of antigen-binding molecules from single cells |
| US20250257399A1 (en) | 2022-10-03 | 2025-08-14 | 10X Genomics, Inc. | Compositions and methods for analyzing genomic insertion sites of exogenous nucleic acids |
| WO2024081212A1 (en) * | 2022-10-10 | 2024-04-18 | 10X Genomics, Inc. | In vitro transcription of spatially captured nucleic acids |
| KR20250094720A (ko) | 2023-03-09 | 2025-06-25 | 이뮤노제네텍스 가부시키가이샤 | 상보 dna쇄를 증폭하는 방법 |
| WO2024238992A1 (en) | 2023-05-18 | 2024-11-21 | 10X Genomics, Inc. | Engineered non-strand displacing family b polymerases for reverse transcription and gap-fill applications |
| WO2024243444A1 (en) | 2023-05-23 | 2024-11-28 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
| WO2024258911A1 (en) | 2023-06-13 | 2024-12-19 | 10X Genomics, Inc. | Sequence specific dna binding proteins |
| WO2025064703A1 (en) | 2023-09-21 | 2025-03-27 | 10X Genomics, Inc. | Compositions and methods for particle hashing |
| WO2025096581A1 (en) | 2023-10-31 | 2025-05-08 | 10X Genomics, Inc. | Methods of spatial analysis and single nuclei sequencing |
| WO2025111369A1 (en) | 2023-11-21 | 2025-05-30 | 10X Genomics, Inc. | Probe-based analysis of polynucleotides and proteins |
| WO2025166072A1 (en) | 2024-02-01 | 2025-08-07 | 10X Genomics, Inc. | Compositions and methods for sample analysis |
| WO2025166185A1 (en) | 2024-02-02 | 2025-08-07 | 10X Genomics, Inc. | Methods of crispr guide rna sequencing in single cell workflows |
Family Cites Families (817)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2797149A (en) | 1953-01-08 | 1957-06-25 | Technicon International Ltd | Methods of and apparatus for analyzing liquids containing crystalloid and non-crystalloid constituents |
| US3047367A (en) | 1959-12-01 | 1962-07-31 | Technicon Instr | Automatic analysis with fluid segmentation |
| US3479141A (en) | 1967-05-17 | 1969-11-18 | Technicon Corp | Method and apparatus for analysis |
| US4124638A (en) | 1977-09-12 | 1978-11-07 | Hansen John N | Solubilizable polyacrylamide gels containing disulfide cross-linkages |
| US4253846A (en) | 1979-11-21 | 1981-03-03 | Technicon Instruments Corporation | Method and apparatus for automated analysis of fluid samples |
| GB2097692B (en) | 1981-01-10 | 1985-05-22 | Shaw Stewart P D | Combining chemical reagents |
| DE3230289A1 (de) | 1982-08-14 | 1984-02-16 | Bayer Ag, 5090 Leverkusen | Herstellung von pharmazeutischen oder kosmetischen dispersionen |
| US4582802A (en) | 1983-09-30 | 1986-04-15 | The United States Of America As Represented By The Department Of Health And Human Services | Stimulation of enzymatic ligation of DNA by high concentrations of nonspecific polymers |
| JPS60227826A (ja) | 1984-04-27 | 1985-11-13 | Sogo Yatsukou Kk | pHに応答するグラフトカプセル |
| KR890003947B1 (ko) | 1985-12-11 | 1989-10-13 | 가부시기가이샤 시마즈세이사구쇼 | 세포융합장치 |
| US4916070A (en) | 1986-04-14 | 1990-04-10 | The General Hospital Corporation | Fibrin-specific antibodies and method of screening for the antibodies |
| US5618711A (en) | 1986-08-22 | 1997-04-08 | Hoffmann-La Roche Inc. | Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase |
| US4872895A (en) | 1986-12-11 | 1989-10-10 | American Telephone And Telegraph Company, At&T Bell Laboratories | Method for fabricating articles which include high silica glass bodies |
| US5202231A (en) | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
| US5525464A (en) | 1987-04-01 | 1996-06-11 | Hyseq, Inc. | Method of sequencing by hybridization of oligonucleotide probes |
| US5149625A (en) | 1987-08-11 | 1992-09-22 | President And Fellows Of Harvard College | Multiplex analysis of DNA |
| US5137829A (en) | 1987-10-05 | 1992-08-11 | Washington University | DNA transposon TN5SEQ1 |
| US5185099A (en) | 1988-04-20 | 1993-02-09 | Institut National De Recherche Chimique Appliquee | Visco-elastic, isotropic materials based on water, fluorinate sufactants and fluorinated oils, process for their preparation, and their use in various fields, such as optics, pharmacology and electrodynamics |
| US5237016A (en) | 1989-01-05 | 1993-08-17 | Siska Diagnostics, Inc. | End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids |
| US6176962B1 (en) | 1990-02-28 | 2001-01-23 | Aclara Biosciences, Inc. | Methods for fabricating enclosed microchannel structures |
| US5756334A (en) | 1990-04-26 | 1998-05-26 | New England Biolabs, Inc. | Thermostable DNA polymerase from 9°N-7 and methods for producing the same |
| ATE176002T1 (de) | 1990-07-24 | 1999-02-15 | Hoffmann La Roche | Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen |
| US5489523A (en) | 1990-12-03 | 1996-02-06 | Stratagene | Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I |
| US6582908B2 (en) | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| US5270183A (en) | 1991-02-08 | 1993-12-14 | Beckman Research Institute Of The City Of Hope | Device and method for the automated cycling of solutions between two or more temperatures |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| ES2094920T3 (es) | 1991-07-04 | 1997-02-01 | Immunodex K S | Reactivos y conjugados solubles en agua a base de polimeros que comprenden restos derivados de divinil sulfona. |
| DE69228247T2 (de) | 1991-08-10 | 1999-07-08 | Medical Research Council, London | Behandlung von Zellpopulationen |
| EP0773227A1 (en) | 1991-09-18 | 1997-05-14 | Affymax Technologies N.V. | Diverse collections of oligomers in use to prepare drugs, diagnostic reagents, pesticides or herbicides |
| US5413924A (en) | 1992-02-13 | 1995-05-09 | Kosak; Kenneth M. | Preparation of wax beads containing a reagent for release by heating |
| AU3816993A (en) | 1992-03-19 | 1993-10-21 | Regents Of The University Of California, The | Multiple tag labeling method for DNA sequencing |
| ATE174813T1 (de) | 1992-05-01 | 1999-01-15 | Univ Pennsylvania | Polynukleotide amplifikationsanalyse mit einer mikrofabrizierten vorrichtung |
| US5587128A (en) | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
| US5840865A (en) | 1992-09-14 | 1998-11-24 | Institute Of Molecular Biology And Biotechnology/Forth | Eukaryotic transposable element |
| US5897783A (en) | 1992-09-24 | 1999-04-27 | Amersham International Plc | Magnetic separation method |
| US5569364A (en) | 1992-11-05 | 1996-10-29 | Soane Biosciences, Inc. | Separation media for electrophoresis |
| AU673245B2 (en) | 1993-02-01 | 1996-10-31 | Seq, Ltd. | Methods and apparatus for DNA sequencing |
| AU6175594A (en) | 1993-02-16 | 1994-09-14 | Alliance Pharmaceutical Corporation | Method of microemulsifying fluorinated oils |
| WO1994023699A1 (en) | 1993-04-19 | 1994-10-27 | Medisorb Technologies International L.P. | Long-acting treatment by slow-release delivery of antisense oligodeoxyribonucleotides from biodegradable microparticles |
| US5456986A (en) | 1993-06-30 | 1995-10-10 | Carnegie Mellon University | Magnetic metal or metal carbide nanoparticles and a process for forming same |
| ATE208658T1 (de) | 1993-07-28 | 2001-11-15 | Pe Corp Ny | Vorrichtung und verfahren zur nukleinsäurevervielfältigung |
| EP0711303B2 (en) | 1993-07-30 | 2009-06-10 | Affymax, Inc. | Biotinylation of proteins |
| EP0723549B1 (en) | 1993-08-30 | 2003-12-17 | Promega Corporation | Nucleic acid purification compositions and methods |
| US5512131A (en) | 1993-10-04 | 1996-04-30 | President And Fellows Of Harvard College | Formation of microstamped patterns on surfaces and derivative articles |
| US20030044777A1 (en) | 1993-10-28 | 2003-03-06 | Kenneth L. Beattie | Flowthrough devices for multiple discrete binding reactions |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5558071A (en) | 1994-03-07 | 1996-09-24 | Combustion Electromagnetics, Inc. | Ignition system power converter and controller |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| JP3339026B2 (ja) | 1994-05-11 | 2002-10-28 | ジェネラ テクノロジーズ リミテッド | 液体から種を捕獲する方法及び分析手法 |
| US5705628A (en) | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US5585069A (en) | 1994-11-10 | 1996-12-17 | David Sarnoff Research Center, Inc. | Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis |
| EP0812434B1 (en) | 1995-03-01 | 2013-09-18 | President and Fellows of Harvard College | Microcontact printing on surfaces and derivative articles |
| US5700642A (en) | 1995-05-22 | 1997-12-23 | Sri International | Oligonucleotide sizing using immobilized cleavable primers |
| WO1996041011A1 (en) | 1995-06-07 | 1996-12-19 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| PL324000A1 (en) | 1995-06-07 | 1998-04-27 | Lynx Therapeutics | Oligionucleotidic labels for sorting and identofication purpose |
| US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US6866760B2 (en) | 1998-08-27 | 2005-03-15 | E Ink Corporation | Electrophoretic medium and process for the production thereof |
| US5872010A (en) | 1995-07-21 | 1999-02-16 | Northeastern University | Microscale fluid handling system |
| US6057149A (en) | 1995-09-15 | 2000-05-02 | The University Of Michigan | Microscale devices and reactions in microscale devices |
| US5851769A (en) | 1995-09-27 | 1998-12-22 | The Regents Of The University Of California | Quantitative DNA fiber mapping |
| US5736330A (en) | 1995-10-11 | 1998-04-07 | Luminex Corporation | Method and compositions for flow cytometric determination of DNA sequences |
| US6051377A (en) | 1995-11-30 | 2000-04-18 | Pharmaseq, Inc. | Multiplex assay for nucleic acids employing transponders |
| US6001571A (en) | 1995-11-30 | 1999-12-14 | Mandecki; Wlodek | Multiplex assay for nucleic acids employing transponders |
| US5736332A (en) | 1995-11-30 | 1998-04-07 | Mandecki; Wlodek | Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders |
| US6355198B1 (en) | 1996-03-15 | 2002-03-12 | President And Fellows Of Harvard College | Method of forming articles including waveguides via capillary micromolding and microtransfer molding |
| WO1997039359A1 (en) | 1996-04-15 | 1997-10-23 | Dade International Inc. | Apparatus and method for analysis |
| CA2255774C (en) | 1996-05-29 | 2008-03-18 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| US5846727A (en) | 1996-06-06 | 1998-12-08 | Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College | Microsystem for rapid DNA sequencing |
| DE69707288T2 (de) | 1996-07-15 | 2002-07-18 | Calcitech Ltd., Hamilton | Herstellung von pulvern |
| US5965443A (en) | 1996-09-09 | 1999-10-12 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
| US5900481A (en) | 1996-11-06 | 1999-05-04 | Sequenom, Inc. | Bead linkers for immobilizing nucleic acids to solid supports |
| US6133436A (en) | 1996-11-06 | 2000-10-17 | Sequenom, Inc. | Beads bound to a solid support and to nucleic acids |
| EP0946749A1 (en) | 1996-11-20 | 1999-10-06 | The Regents Of The University Of Michigan | Microfabricated isothermal nucleic acid amplification devices and methods |
| US5958703A (en) | 1996-12-03 | 1999-09-28 | Glaxo Group Limited | Use of modified tethers in screening compound libraries |
| US20020172965A1 (en) | 1996-12-13 | 2002-11-21 | Arcaris, Inc. | Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom |
| US20050042625A1 (en) | 1997-01-15 | 2005-02-24 | Xzillion Gmbh & Co. | Mass label linked hybridisation probes |
| US6297006B1 (en) | 1997-01-16 | 2001-10-02 | Hyseq, Inc. | Methods for sequencing repetitive sequences and for determining the order of sequence subfragments |
| US20020034737A1 (en) | 1997-03-04 | 2002-03-21 | Hyseq, Inc. | Methods and compositions for detection or quantification of nucleic acid species |
| CA2281205A1 (en) | 1997-02-12 | 1998-08-13 | Eugene Y. Chan | Methods and products for analyzing polymers |
| US6327410B1 (en) | 1997-03-14 | 2001-12-04 | The Trustees Of Tufts College | Target analyte sensors utilizing Microspheres |
| US7622294B2 (en) | 1997-03-14 | 2009-11-24 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
| US20030027203A1 (en) | 1997-03-24 | 2003-02-06 | Fields Robert E. | Biomolecular processor |
| US6391622B1 (en) | 1997-04-04 | 2002-05-21 | Caliper Technologies Corp. | Closed-loop biochemical analyzers |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| WO1998050583A1 (en) | 1997-05-02 | 1998-11-12 | Gen-Probe Incorporated | Two-step hybridization and capture of a polynucleotide |
| CA2286601A1 (en) | 1997-05-16 | 1998-11-26 | Alberta Research Council | Microfluidic system and methods of use |
| CA2291180A1 (en) | 1997-05-23 | 1998-11-26 | Lynx Therapeutics, Inc. | System and apparatus for sequential processing of analytes |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| US20040241759A1 (en) | 1997-06-16 | 2004-12-02 | Eileen Tozer | High throughput screening of libraries |
| EP1908832B1 (en) | 1997-07-07 | 2012-12-26 | Medical Research Council | A method for increasing the concentration of a nucleic acid molecule |
| GB9714716D0 (en) | 1997-07-11 | 1997-09-17 | Brax Genomics Ltd | Characterising nucleic acids |
| FI103809B1 (fi) | 1997-07-14 | 1999-09-30 | Finnzymes Oy | In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten |
| US20050037397A1 (en) | 2001-03-28 | 2005-02-17 | Nanosphere, Inc. | Bio-barcode based detection of target analytes |
| US6974669B2 (en) | 2000-03-28 | 2005-12-13 | Nanosphere, Inc. | Bio-barcodes based on oligonucleotide-modified nanoparticles |
| US6368871B1 (en) | 1997-08-13 | 2002-04-09 | Cepheid | Non-planar microstructures for manipulation of fluid samples |
| CA2300940A1 (en) | 1997-08-15 | 1999-02-25 | Hyseq, Inc. | Methods and compositions for detection or quantification of nucleic acid species |
| WO1999014368A2 (en) | 1997-09-15 | 1999-03-25 | Whitehead Institute For Biomedical Research | Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device |
| US20020092767A1 (en) | 1997-09-19 | 2002-07-18 | Aclara Biosciences, Inc. | Multiple array microfluidic device units |
| US7214298B2 (en) | 1997-09-23 | 2007-05-08 | California Institute Of Technology | Microfabricated cell sorter |
| AU9673198A (en) | 1997-10-02 | 1999-04-27 | Aclara Biosciences, Inc. | Capillary assays involving separation of free and bound species |
| US5842787A (en) | 1997-10-09 | 1998-12-01 | Caliper Technologies Corporation | Microfluidic systems incorporating varied channel dimensions |
| US6485944B1 (en) | 1997-10-10 | 2002-11-26 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| EP1028970A1 (en) | 1997-10-10 | 2000-08-23 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| US6511803B1 (en) | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| KR20010031140A (ko) | 1997-10-14 | 2001-04-16 | 루미넥스 코포레이션 | 정밀 형광염료 입자 및 그의 제조방법 그리고 그의 사용 |
| JP2001521169A (ja) | 1997-10-24 | 2001-11-06 | ノースイースタン・ユニバーシティ | 広範囲の採集と分析とで高スループットの調整分離のためのマルチチャンネルの微小規模システム |
| US6913935B1 (en) | 1997-12-04 | 2005-07-05 | Amersham Biosciences Uk Limited | Multiple assay method |
| AU1726199A (en) | 1997-12-31 | 1999-07-19 | Chiron Corporation | Metastatic cancer regulated gene |
| JP2002504342A (ja) | 1998-02-19 | 2002-02-12 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 一価mhc結合ドメイン融合タンパク質および接合体、多価mhc結合ドメイン融合タンパク質および接合体、ならびに多量体mhc結合ドメイン融合タンパク質および接合体、そしてそれらのための使用 |
| AU3196099A (en) | 1998-03-27 | 1999-10-18 | President And Fellows Of Harvard College | Systematic identification of essential genes by (in vitro) transposon mutagenesis |
| US6022716A (en) | 1998-04-10 | 2000-02-08 | Genset Sa | High throughput DNA sequencing vector |
| WO1999052708A1 (en) | 1998-04-13 | 1999-10-21 | Luminex Corporation | Liquid labeling with fluorescent microparticles |
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US5997636A (en) | 1998-05-01 | 1999-12-07 | Instrumentation Technology Associates, Inc. | Method and apparatus for growing crystals |
| US6123798A (en) | 1998-05-06 | 2000-09-26 | Caliper Technologies Corp. | Methods of fabricating polymeric structures incorporating microscale fluidic elements |
| US6534262B1 (en) | 1998-05-14 | 2003-03-18 | Whitehead Institute For Biomedical Research | Solid phase technique for selectively isolating nucleic acids |
| US6306590B1 (en) | 1998-06-08 | 2001-10-23 | Caliper Technologies Corp. | Microfluidic matrix localization apparatus and methods |
| EP2360271A1 (en) | 1998-06-24 | 2011-08-24 | Illumina, Inc. | Decoding of array sensors with microspheres |
| CA2339734A1 (en) | 1998-08-07 | 2000-02-17 | Jan Trnovsky | Gel microdrops in genetic analysis |
| US6159736A (en) | 1998-09-23 | 2000-12-12 | Wisconsin Alumni Research Foundation | Method for making insertional mutations using a Tn5 synaptic complex |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| US6296020B1 (en) | 1998-10-13 | 2001-10-02 | Biomicro Systems, Inc. | Fluid circuit components based upon passive fluid dynamics |
| US6489096B1 (en) | 1998-10-15 | 2002-12-03 | Princeton University | Quantitative analysis of hybridization patterns and intensities in oligonucleotide arrays |
| SE9803614L (sv) | 1998-10-19 | 2000-04-20 | Muhammed Mamoun | Förfarande och anordning för framställning av nanopartiklar |
| WO2000026412A1 (en) | 1998-11-02 | 2000-05-11 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
| US5942609A (en) | 1998-11-12 | 1999-08-24 | The Porkin-Elmer Corporation | Ligation assembly and detection of polynucleotides on solid-support |
| US6569631B1 (en) | 1998-11-12 | 2003-05-27 | 3-Dimensional Pharmaceuticals, Inc. | Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes |
| AU2206800A (en) | 1998-12-11 | 2000-06-26 | Regents Of The University Of California, The | Targeted molecular bar codes and methods for using the same |
| NO986133D0 (no) | 1998-12-23 | 1998-12-23 | Preben Lexow | FremgangsmÕte for DNA-sekvensering |
| GB9900298D0 (en) | 1999-01-07 | 1999-02-24 | Medical Res Council | Optical sorting method |
| US6416642B1 (en) | 1999-01-21 | 2002-07-09 | Caliper Technologies Corp. | Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection |
| US6635419B1 (en) | 1999-02-16 | 2003-10-21 | Applera Corporation | Polynucleotide sequencing method |
| US20030027214A1 (en) | 1999-02-17 | 2003-02-06 | Kamb Carl Alexander | Methods for substrate-ligand interaction screening |
| ATE556149T1 (de) | 1999-02-23 | 2012-05-15 | Caliper Life Sciences Inc | Manipulation von mikropartikeln in mikrofluidischen systemen |
| US7615373B2 (en) | 1999-02-25 | 2009-11-10 | Virginia Commonwealth University Intellectual Property Foundation | Electroprocessed collagen and tissue engineering |
| US6171850B1 (en) | 1999-03-08 | 2001-01-09 | Caliper Technologies Corp. | Integrated devices and systems for performing temperature controlled reactions and analyses |
| US6303343B1 (en) | 1999-04-06 | 2001-10-16 | Caliper Technologies Corp. | Inefficient fast PCR |
| US6908737B2 (en) | 1999-04-15 | 2005-06-21 | Vitra Bioscience, Inc. | Systems and methods of conducting multiplexed experiments |
| US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| CA2370976C (en) | 1999-04-20 | 2009-10-20 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| WO2000065042A1 (en) | 1999-04-28 | 2000-11-02 | The Board Of Trustees Of The Leland Stanford Junior University | P element derived vector and methods for its use |
| CA2373537A1 (en) | 1999-05-12 | 2000-11-16 | Aclara Biosciences, Inc. | Multiplexed fluorescent detection in microfluidic devices |
| WO2000070095A2 (en) * | 1999-05-17 | 2000-11-23 | Dade Behring Inc. | Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide |
| US20020051971A1 (en) | 1999-05-21 | 2002-05-02 | John R. Stuelpnagel | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
| US6846622B1 (en) | 1999-05-26 | 2005-01-25 | Oregon Health & Science University | Tagged epitope protein transposable element |
| US20030124509A1 (en) | 1999-06-03 | 2003-07-03 | Kenis Paul J.A. | Laminar flow patterning and articles made thereby |
| US6372813B1 (en) | 1999-06-25 | 2002-04-16 | Motorola | Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays |
| AU6068300A (en) | 1999-07-06 | 2001-01-22 | Caliper Technologies Corporation | Microfluidic systems and methods for determining modulator kinetics |
| US6977145B2 (en) | 1999-07-28 | 2005-12-20 | Serono Genetics Institute S.A. | Method for carrying out a biochemical protocol in continuous flow in a microreactor |
| US6524456B1 (en) | 1999-08-12 | 2003-02-25 | Ut-Battelle, Llc | Microfluidic devices for the controlled manipulation of small volumes |
| WO2001013086A2 (en) | 1999-08-13 | 2001-02-22 | Brandeis University | Detection of nucleic acids |
| AU6788100A (en) | 1999-08-20 | 2001-03-19 | Luminex Corporation | Liquid array technology |
| DE60040794D1 (de) | 1999-08-27 | 2008-12-24 | Matrix Technologies Corp | Verfahren zur Immobilisierung von Oligonukleotiden auf festem Trägermaterial |
| US6982146B1 (en) | 1999-08-30 | 2006-01-03 | The United States Of America As Represented By The Department Of Health And Human Services | High speed parallel molecular nucleic acid sequencing |
| CN1425133A (zh) | 1999-10-13 | 2003-06-18 | 西格雷特生物科学有限公司 | 检测和识别试样中分子事件的系统和方法 |
| US6958225B2 (en) | 1999-10-27 | 2005-10-25 | Affymetrix, Inc. | Complexity management of genomic DNA |
| AU1100201A (en) | 1999-10-28 | 2001-05-08 | Board Of Trustees Of The Leland Stanford Junior University | Methods of in vivo gene transfer using a sleeping beauty transposon system |
| JP4721603B2 (ja) | 1999-11-08 | 2011-07-13 | 栄研化学株式会社 | 変異および/または多型の検出方法 |
| US6432290B1 (en) | 1999-11-26 | 2002-08-13 | The Governors Of The University Of Alberta | Apparatus and method for trapping bead based reagents within microfluidic analysis systems |
| US20010051348A1 (en) | 2000-01-28 | 2001-12-13 | Lee Chee Wee | Novel ligands and methods for preparing same |
| ATE382858T1 (de) | 2000-02-23 | 2008-01-15 | Caliper Life Sciences Inc | Mehrfach-reservoir-drucksteuersystem |
| US6720157B2 (en) | 2000-02-23 | 2004-04-13 | Zyomyx, Inc. | Chips having elevated sample surfaces |
| IL134830A0 (en) | 2000-03-01 | 2001-05-20 | Chay 13 Medical Res Group N V | Peptides and immunostimulatory and anti-bacterial pharmaceutical compositions containing them |
| MXPA02009031A (es) | 2000-03-14 | 2004-08-19 | Amylin Pharmaceuticals Inc | Efectos del peptido-1(7-36) tipo glucagon en la motilidad antro-piloro.duodenal. |
| US6376191B1 (en) | 2000-03-22 | 2002-04-23 | Mergen, Ltd. | Microarray-based analysis of polynucleotide sequence variations |
| CA2404008A1 (en) | 2000-03-31 | 2001-10-11 | Jurgen Sygusch | Protein crystallization in microfluidic structures |
| EP1275005A1 (en) | 2000-04-06 | 2003-01-15 | Caliper Technologies Corporation | Methods and devices for achieving long incubation times in high-throughput systems |
| AU2001253310A1 (en) | 2000-04-10 | 2001-10-23 | Matthew Ashby | Methods for the survey and genetic analysis of populations |
| US6481453B1 (en) | 2000-04-14 | 2002-11-19 | Nanostream, Inc. | Microfluidic branch metering systems and methods |
| US6800298B1 (en) | 2000-05-11 | 2004-10-05 | Clemson University | Biological lubricant composition and method of applying lubricant composition |
| US20060008799A1 (en) | 2000-05-22 | 2006-01-12 | Hong Cai | Rapid haplotyping by single molecule detection |
| CA2408574A1 (en) | 2000-05-24 | 2001-11-29 | Micronics, Inc. | Microfluidic concentration gradient loop |
| US6645432B1 (en) | 2000-05-25 | 2003-11-11 | President & Fellows Of Harvard College | Microfluidic systems including three-dimensionally arrayed channel networks |
| US20060263888A1 (en) | 2000-06-02 | 2006-11-23 | Honeywell International Inc. | Differential white blood count on a disposable card |
| US6632606B1 (en) | 2000-06-12 | 2003-10-14 | Aclara Biosciences, Inc. | Methods for single nucleotide polymorphism detection |
| CA2635452A1 (en) | 2000-06-21 | 2001-12-27 | Bioarray Solutions, Ltd. | Looped probe design to control hybridization stringency |
| AU2001281076A1 (en) | 2000-08-07 | 2002-02-18 | Nanostream, Inc. | Fluidic mixer in microfluidic system |
| US6773566B2 (en) | 2000-08-31 | 2004-08-10 | Nanolytics, Inc. | Electrostatic actuators for microfluidics and methods for using same |
| US6610499B1 (en) | 2000-08-31 | 2003-08-26 | The Regents Of The University Of California | Capillary array and related methods |
| AU2001290867A1 (en) | 2000-09-14 | 2002-03-26 | Caliper Technologies Corp. | Microfluidic devices and methods for performing temperature mediated reactions |
| EP1334347A1 (en) | 2000-09-15 | 2003-08-13 | California Institute Of Technology | Microfabricated crossflow devices and methods |
| EP1322936A2 (en) | 2000-10-03 | 2003-07-02 | California Institute Of Technology | Microfluidic devices and methods of use |
| WO2002031203A2 (en) | 2000-10-10 | 2002-04-18 | Diversa Corporation | High throughput or capillary-based screening for a bioactivity or biomolecule |
| US7045283B2 (en) | 2000-10-18 | 2006-05-16 | The Regents Of The University Of California | Methods of high-throughput screening for internalizing antibodies |
| JP2002155305A (ja) | 2000-11-14 | 2002-05-31 | Akira Kawasaki | 単分散粒子の製造装置及び単分散粒子の製造方法及びその製造方法で製造された単分散粒子 |
| US6492154B2 (en) | 2001-01-31 | 2002-12-10 | Applera Corporation | Isolated human kinase proteins, nucleic acid molecules encoding human kinase proteins, and uses thereof |
| CA2332186A1 (en) | 2001-02-08 | 2002-08-08 | Her Majesty In Right Of Canada As Represented By The Minister Of Agricul Ture And Agri-Food Canada | Replicative in vivo gene targeting |
| US7670559B2 (en) | 2001-02-15 | 2010-03-02 | Caliper Life Sciences, Inc. | Microfluidic systems with enhanced detection systems |
| ATE312076T1 (de) | 2001-02-22 | 2005-12-15 | Anika Therapeutics Inc | Thiol-modifizierte hyaluronan-derivate |
| EP1510251B1 (en) | 2001-02-23 | 2006-09-06 | Japan Science and Technology Agency | Process and apparatus for producing microcapsules |
| US20150329617A1 (en) | 2001-03-14 | 2015-11-19 | Dynal Biotech Asa | Novel MHC molecule constructs, and methods of employing these constructs for diagnosis and therapy, and uses of MHC molecules |
| US7211654B2 (en) | 2001-03-14 | 2007-05-01 | Regents Of The University Of Michigan | Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports |
| US20060040286A1 (en) | 2001-03-28 | 2006-02-23 | Nanosphere, Inc. | Bio-barcode based detection of target analytes |
| US20020150502A1 (en) | 2001-04-03 | 2002-10-17 | Weigl Bernhard H. | Surface tension reduction channel |
| US7138267B1 (en) | 2001-04-04 | 2006-11-21 | Epicentre Technologies Corporation | Methods and compositions for amplifying DNA clone copy number |
| US20030027221A1 (en) | 2001-04-06 | 2003-02-06 | Scott Melissa E. | High-throughput screening assays by encapsulation |
| US7572642B2 (en) | 2001-04-18 | 2009-08-11 | Ambrigen, Llc | Assay based on particles, which specifically bind with targets in spatially distributed characteristic patterns |
| EP1399580B1 (en) | 2001-05-26 | 2008-10-08 | One Cell Systems, Inc. | Secretion of proteins by encapsulated cells |
| US6880576B2 (en) | 2001-06-07 | 2005-04-19 | Nanostream, Inc. | Microfluidic devices for methods development |
| US7179423B2 (en) | 2001-06-20 | 2007-02-20 | Cytonome, Inc. | Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system |
| US7262063B2 (en) | 2001-06-21 | 2007-08-28 | Bio Array Solutions, Ltd. | Directed assembly of functional heterostructures |
| US6613523B2 (en) | 2001-06-29 | 2003-09-02 | Agilent Technologies, Inc. | Method of DNA sequencing using cleavable tags |
| US7077152B2 (en) | 2001-07-07 | 2006-07-18 | Nanostream, Inc. | Microfluidic metering systems and methods |
| WO2003008437A2 (en) | 2001-07-20 | 2003-01-30 | California Institute Of Technology | Protein and nucleic acid expression systems |
| US6767731B2 (en) | 2001-08-27 | 2004-07-27 | Intel Corporation | Electron induced fluorescent method for nucleic acid sequencing |
| WO2003031591A2 (en) * | 2001-10-10 | 2003-04-17 | Superarray Bioscience Corporation | Detecting targets by unique identifier nucleotide tags |
| US7297485B2 (en) | 2001-10-15 | 2007-11-20 | Qiagen Gmbh | Method for nucleic acid amplification that results in low amplification bias |
| AU2002360282A1 (en) | 2001-10-19 | 2003-06-10 | West Virginia University Research Corporation | Microflludic system for proteome analysis |
| US6783647B2 (en) | 2001-10-19 | 2004-08-31 | Ut-Battelle, Llc | Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate |
| US20030149307A1 (en) | 2001-10-24 | 2003-08-07 | Baxter International Inc. | Process for the preparation of polyethylene glycol bis amine |
| US20040195728A1 (en) | 2001-10-26 | 2004-10-07 | Dennis Slomski | System and method for injection molded micro-replication of micro-fluidic substrates |
| US20030182068A1 (en) | 2001-10-30 | 2003-09-25 | Battersby Bronwyn J. | Device and methods for directed synthesis of chemical libraries |
| US7262056B2 (en) | 2001-11-08 | 2007-08-28 | Mirus Bio Corporation | Enhancing intermolecular integration of nucleic acids using integrator complexes |
| GB0127564D0 (en) | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
| EP1456409B1 (en) | 2001-11-28 | 2010-02-24 | Bio-Rad Laboratories, Inc. | Parallel polymorphism scoring by amplification and error correction |
| US7335153B2 (en) | 2001-12-28 | 2008-02-26 | Bio Array Solutions Ltd. | Arrays of microparticles and methods of preparation thereof |
| WO2003057010A2 (en) | 2002-01-04 | 2003-07-17 | Board Of Regents, The University Of Texas System | Droplet-based microfluidic oligonucleotide synthesis engine |
| CA2473376A1 (en) | 2002-01-16 | 2003-07-31 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
| KR100459870B1 (ko) | 2002-02-22 | 2004-12-04 | 한국과학기술원 | 트랜스포존과 Cre/loxP 부위 특이적 재조합 방법을 이용하는 염색체의 특정부위가 제거된 미생물 변이주 제조방법 |
| AU2003226679A1 (en) | 2002-03-20 | 2003-09-29 | Innovativebio.Biz | Microcapsules with controlable permeability encapsulating a nucleic acid amplification reaction mixture and their use as reaction compartments for parallels reactions |
| US7901939B2 (en) | 2002-05-09 | 2011-03-08 | University Of Chicago | Method for performing crystallization and reactions in pressure-driven fluid plugs |
| EP2283917B1 (en) | 2002-05-09 | 2021-12-15 | The University of Chicago | Device for pressure-driven plug transport and reaction |
| US7527966B2 (en) | 2002-06-26 | 2009-05-05 | Transgenrx, Inc. | Gene regulation in transgenic animals using a transposon-based vector |
| JP2006507921A (ja) | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 流体分散のための方法および装置 |
| WO2004010106A2 (en) | 2002-07-24 | 2004-01-29 | Ptc Therapeutics, Inc. | METHODS FOR IDENTIFYING SMALL MOLEDULES THAT MODULATE PREMATURE TRANSLATION TERMINATION AND NONSENSE MEDIATED mRNA DECAY |
| IL151660A0 (en) | 2002-09-09 | 2003-04-10 | Univ Ben Gurion | Method for isolating and culturing unculturable microorganisms |
| US7919235B2 (en) | 2002-09-30 | 2011-04-05 | F. Hoffman-La Roche Ag | Oligonucleotides for genotyping thymidylate synthase gene |
| US20040081962A1 (en) | 2002-10-23 | 2004-04-29 | Caifu Chen | Methods for synthesizing complementary DNA |
| US6979713B2 (en) | 2002-11-25 | 2005-12-27 | 3M Innovative Properties Company | Curable compositions and abrasive articles therefrom |
| US20050266582A1 (en) | 2002-12-16 | 2005-12-01 | Modlin Douglas N | Microfluidic system with integrated permeable membrane |
| US20040248299A1 (en) | 2002-12-27 | 2004-12-09 | Sumedha Jayasena | RNA interference |
| ATE435925T1 (de) | 2003-01-17 | 2009-07-15 | Univ Boston | Haplotypanalyse |
| DE602004026033D1 (de) | 2003-01-29 | 2010-04-29 | 454 Corp | Zweiendige sequenzierung |
| US8420386B2 (en) | 2003-02-10 | 2013-04-16 | Max-Delbruck-Centrum Fur Molekulare Medizin (Mdc) | Transposon-based targeting system |
| US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
| US7316903B2 (en) | 2003-03-28 | 2008-01-08 | United States Of America As Represented By The Department Of Health And Human Services | Detection of nucleic acid sequence variations using phase Mu transposase |
| GB0307403D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Selection by compartmentalised screening |
| US20060078893A1 (en) | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
| GB0307428D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Compartmentalised combinatorial chemistry |
| CA2521051C (en) | 2003-04-04 | 2012-03-20 | Pfizer Products Inc. | Microfluidized oil-in-water emulsions and vaccine compositions |
| US20100035254A1 (en) | 2003-04-08 | 2010-02-11 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| EP2127736A1 (en) | 2003-04-10 | 2009-12-02 | The President and Fellows of Harvard College | Formation and control of fluidic species |
| EP1629286A1 (en) | 2003-05-16 | 2006-03-01 | Global Technologies (NZ) Ltd. | Method and apparatus for mixing sample and reagent in a suspension fluid |
| DE112004001376D2 (de) | 2003-05-19 | 2006-04-13 | Knoell Hans Forschung Ev | Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten und zum zudosieren von Reaktionsflüssigkeiten zu in Separationsmedium eingebetteten Flüssigkeitskompartimenten |
| WO2004105734A1 (en) | 2003-05-28 | 2004-12-09 | Valorisation Recherche, Societe En Commandite | Method of preparing microcapsules |
| GB0313170D0 (en) | 2003-06-09 | 2003-07-16 | Qinetiq Ltd | Method and apparatus for spore disruption and/or detection |
| CA2529285A1 (en) | 2003-06-13 | 2004-12-29 | The General Hospital Corporation | Microfluidic systems for size based removal of red blood cells and platelets from blood |
| CN101001960A (zh) | 2003-06-27 | 2007-07-18 | 西北大学 | 基于生物条形码检测靶分析物 |
| GB2403475B (en) | 2003-07-01 | 2008-02-06 | Oxitec Ltd | Stable integrands |
| GB0315438D0 (en) | 2003-07-02 | 2003-08-06 | Univ Manchester | Analysis of mixed cell populations |
| EP1641809B2 (en) | 2003-07-05 | 2018-10-03 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
| JP4630870B2 (ja) | 2003-08-27 | 2011-02-09 | プレジデント アンド フェロウズ オブ ハーバード カレッジ | 流体種の電子的制御 |
| JP4988345B2 (ja) | 2003-09-04 | 2012-08-01 | ザ・ユナイテッド・ステイツ・オブ・アメリカ・アズ・リプレゼンティッド・バイ・ザ・デパートメント・オブ・ヴェテランズ・アフェアーズ | 眼用ハイドロゲルナノコンポジット |
| EP1663497B2 (en) | 2003-09-05 | 2020-03-25 | Stokes Bio Limited | A microfluidic analysis system |
| US7354706B2 (en) | 2003-09-09 | 2008-04-08 | The Regents Of The University Of Colorado, A Body Corporate | Use of photopolymerization for amplification and detection of a molecular recognition event |
| WO2005028646A1 (ja) | 2003-09-22 | 2005-03-31 | Riken | 効率的なdna逆位反復構造の調製方法 |
| DK1691196T3 (da) | 2003-09-25 | 2013-04-15 | Vivalis | Chip med mikrobrøndsarray og fremgangsmåde til fremstilling heraf |
| JP2007512811A (ja) | 2003-11-10 | 2007-05-24 | インベスチゲン, インコーポレイテッド | 検出のための核酸を調製する方法 |
| EP1691792A4 (en) | 2003-11-24 | 2008-05-28 | Yeda Res & Dev | COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES |
| US20050136417A1 (en) | 2003-12-19 | 2005-06-23 | Affymetrix, Inc. | Amplification of nucleic acids |
| US8071364B2 (en) | 2003-12-24 | 2011-12-06 | Transgenrx, Inc. | Gene therapy using transposon-based vectors |
| US7927797B2 (en) | 2004-01-28 | 2011-04-19 | 454 Life Sciences Corporation | Nucleic acid amplification with continuous flow emulsion |
| US20050181379A1 (en) | 2004-02-18 | 2005-08-18 | Intel Corporation | Method and device for isolating and positioning single nucleic acid molecules |
| CA2557841A1 (en) | 2004-02-27 | 2005-09-09 | President And Fellows Of Harvard College | Polony fluorescent in situ sequencing beads |
| US20100216153A1 (en) | 2004-02-27 | 2010-08-26 | Helicos Biosciences Corporation | Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities |
| KR100552706B1 (ko) | 2004-03-12 | 2006-02-20 | 삼성전자주식회사 | 핵산 증폭 방법 및 장치 |
| JP4777238B2 (ja) | 2004-03-23 | 2011-09-21 | 独立行政法人科学技術振興機構 | 微小液滴の生成方法及び装置 |
| US20050221339A1 (en) | 2004-03-31 | 2005-10-06 | Medical Research Council Harvard University | Compartmentalised screening by microfluidic control |
| US20060020371A1 (en) | 2004-04-13 | 2006-01-26 | President And Fellows Of Harvard College | Methods and apparatus for manipulation and/or detection of biological samples and other objects |
| US20050250147A1 (en) | 2004-05-10 | 2005-11-10 | Macevicz Stephen C | Digital profiling of polynucleotide populations |
| WO2006047183A2 (en) | 2004-10-21 | 2006-05-04 | New England Biolabs, Inc. | Recombinant dna nicking endonuclease and uses thereof |
| US7799553B2 (en) | 2004-06-01 | 2010-09-21 | The Regents Of The University Of California | Microfabricated integrated DNA analysis system |
| US7700281B2 (en) | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
| US7968085B2 (en) | 2004-07-05 | 2011-06-28 | Ascendis Pharma A/S | Hydrogel formulations |
| CN1648671B (zh) | 2005-02-06 | 2012-09-26 | 成都夸常医学工业有限公司 | 多反应器分析芯片检测方法和分析芯片及检测装置 |
| US7608434B2 (en) | 2004-08-04 | 2009-10-27 | Wisconsin Alumni Research Foundation | Mutated Tn5 transposase proteins and the use thereof |
| WO2006030993A1 (en) | 2004-09-14 | 2006-03-23 | Jin-Ho Choy | Information code system using dna sequences |
| US7892731B2 (en) | 2004-10-01 | 2011-02-22 | Radix Biosolutions, Ltd. | System and method for inhibiting the decryption of a nucleic acid probe sequence used for the detection of a specific nucleic acid |
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| WO2007001448A2 (en) | 2004-11-04 | 2007-01-04 | Massachusetts Institute Of Technology | Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals |
| WO2006051552A2 (en) | 2004-11-15 | 2006-05-18 | Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science | Directed evolution and selection using in vitro compartmentalization |
| US7329493B2 (en) | 2004-12-22 | 2008-02-12 | Asiagen Corporation | One-tube nested PCR for detecting Mycobacterium tuberculosis |
| EP1871527B1 (en) | 2004-12-23 | 2017-09-27 | Abbott Point of Care Inc. | Molecular diagnostics system |
| US20080213593A1 (en) | 2005-01-21 | 2008-09-04 | President And Fellows Of Harvard College | Systems And Methods For Forming Fluidic Droplets Encapsulated In Particles Such As Colloidal Particles |
| US7579153B2 (en) | 2005-01-25 | 2009-08-25 | Population Genetics Technologies, Ltd. | Isothermal DNA amplification |
| US7407757B2 (en) | 2005-02-10 | 2008-08-05 | Population Genetics Technologies | Genetic analysis by sequence-specific sorting |
| US7393665B2 (en) | 2005-02-10 | 2008-07-01 | Population Genetics Technologies Ltd | Methods and compositions for tagging and identifying polynucleotides |
| EP1871903B1 (en) | 2005-02-18 | 2011-12-21 | Canon U.S. Life Sciences, Inc. | Devices and methods for identifying genomic dna of organisms |
| JP4649621B2 (ja) | 2005-02-21 | 2011-03-16 | 国立大学法人 鹿児島大学 | バイオディーゼル燃料の精製方法 |
| US20070054119A1 (en) | 2005-03-04 | 2007-03-08 | Piotr Garstecki | Systems and methods of forming particles |
| WO2006096571A2 (en) | 2005-03-04 | 2006-09-14 | President And Fellows Of Harvard College | Method and apparatus for forming multiple emulsions |
| US9040237B2 (en) | 2005-03-04 | 2015-05-26 | Intel Corporation | Sensor arrays and nucleic acid sequencing applications |
| JP2006289250A (ja) | 2005-04-08 | 2006-10-26 | Kao Corp | マイクロミキサー及びそれを用いた流体混合方法 |
| US8407013B2 (en) | 2005-06-07 | 2013-03-26 | Peter K. Rogan | AB initio generation of single copy genomic probes |
| EP2620510B2 (en) | 2005-06-15 | 2020-02-19 | Complete Genomics Inc. | Single molecule arrays for genetic and chemical analysis |
| US20090264299A1 (en) | 2006-02-24 | 2009-10-22 | Complete Genomics, Inc. | High throughput genome sequencing on DNA arrays |
| JP2006349060A (ja) | 2005-06-16 | 2006-12-28 | Ntn Corp | ボールねじ |
| WO2007002490A2 (en) | 2005-06-22 | 2007-01-04 | The Research Foundation Of State University Of New York | Massively parallel 2-dimensional capillary electrophoresis |
| WO2007002567A2 (en) | 2005-06-23 | 2007-01-04 | Nanosphere, Inc. | Selective isolation and concentration of nucleic acids from complex samples |
| EP1921140B1 (en) | 2005-07-05 | 2011-12-14 | Juridical Foundation The Chemo-Sero-Therapeutic Research Institute | Mutant transposon vector and use thereof |
| JP5051490B2 (ja) | 2005-07-08 | 2012-10-17 | 独立行政法人産業技術総合研究所 | マクロ生体材料を内包する無機マイクロカプセルおよびその製造方法 |
| US20070020640A1 (en) | 2005-07-21 | 2007-01-25 | Mccloskey Megan L | Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis |
| FR2888912B1 (fr) | 2005-07-25 | 2007-08-24 | Commissariat Energie Atomique | Procede de commande d'une communication entre deux zones par electromouillage, dispositif comportant des zones isolables les unes des autres et procede de realisation d'un tel dispositif |
| US20070031857A1 (en) | 2005-08-02 | 2007-02-08 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction |
| DE102005037401B4 (de) | 2005-08-08 | 2007-09-27 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Bildung einer Emulsion in einem fluidischen Mikrosystem |
| WO2007024840A2 (en) | 2005-08-22 | 2007-03-01 | Critical Therapeutics, Inc. | Method of quantitating nucleic acids by flow cytometry microparticle-based array |
| US7556776B2 (en) | 2005-09-08 | 2009-07-07 | President And Fellows Of Harvard College | Microfluidic manipulation of fluids and reactions |
| JP2007074967A (ja) | 2005-09-13 | 2007-03-29 | Canon Inc | 識別子プローブ及びそれを用いた核酸増幅方法 |
| AU2006336290A1 (en) | 2005-09-16 | 2007-07-26 | The Regents Of The University Of California | A colorimetric bio-barcode amplification assay for analyte detection |
| US7960104B2 (en) | 2005-10-07 | 2011-06-14 | Callida Genomics, Inc. | Self-assembled single molecule arrays and uses thereof |
| AU2007249635B2 (en) | 2005-10-07 | 2012-05-31 | Complete Genomics, Inc. | High throughput genome sequencing on DNA arrays |
| US20070111241A1 (en) | 2005-10-14 | 2007-05-17 | Nezih Cereb | System and method for accessing, tracking, and editing sequence analysis and software to accomplish the same |
| US7709544B2 (en) | 2005-10-25 | 2010-05-04 | Massachusetts Institute Of Technology | Microstructure synthesis by flow lithography and polymerization |
| US20070190543A1 (en) | 2005-11-14 | 2007-08-16 | Applera Corporation | Coded Molecules for Detecting Target Analytes |
| US20070134277A1 (en) | 2005-12-09 | 2007-06-14 | Children's Medical Center Corporation | Pharmaceutical formulation for sulfur-containing drugs in liquid dosage forms |
| US20070141584A1 (en) | 2005-12-20 | 2007-06-21 | Roberts Douglas N | Methods for assessment of native chromatin on microarrays |
| US7932037B2 (en) | 2007-12-05 | 2011-04-26 | Perkinelmer Health Sciences, Inc. | DNA assays using amplicon probes on encoded particles |
| CA2636855C (en) | 2006-01-11 | 2016-09-27 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
| WO2007087310A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Nucleic acid analysis using sequence tokens |
| WO2007087312A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Molecular counting |
| EP2263787A3 (en) | 2006-01-27 | 2012-02-22 | President and Fellows of Harvard College | Fluidic droplet coalescence |
| EP2385143B1 (en) | 2006-02-02 | 2016-07-13 | The Board of Trustees of the Leland Stanford Junior University | Non-invasive fetal genetic screening by digital analysis |
| WO2007092538A2 (en) | 2006-02-07 | 2007-08-16 | President And Fellows Of Harvard College | Methods for making nucleotide probes for sequencing and synthesis |
| GB0603251D0 (en) | 2006-02-17 | 2006-03-29 | Isis Innovation | DNA conformation |
| WO2007111937A1 (en) | 2006-03-23 | 2007-10-04 | Applera Corporation | Directed enrichment of genomic dna for high-throughput sequencing |
| JP4921829B2 (ja) | 2006-03-30 | 2012-04-25 | 株式会社東芝 | 微粒子の製造装置、乳化剤保持部、微粒子の製造方法および分子膜の製造方法 |
| WO2007114794A1 (en) | 2006-03-31 | 2007-10-11 | Nam Trung Nguyen | Active control for droplet-based microfluidics |
| EP2007907A2 (en) | 2006-04-19 | 2008-12-31 | Applera Corporation | Reagents, methods, and libraries for gel-free bead-based sequencing |
| US7811603B2 (en) | 2006-05-09 | 2010-10-12 | The Regents Of The University Of California | Microfluidic device for forming monodisperse lipoplexes |
| ATE540750T1 (de) | 2006-05-11 | 2012-01-15 | Raindance Technologies Inc | Mikrofluidische vorrichtung und verfahren |
| ES2620398T3 (es) | 2006-05-22 | 2017-06-28 | Nanostring Technologies, Inc. | Sistemas y métodos para analizar nanoindicadores |
| RU2321638C2 (ru) | 2006-05-23 | 2008-04-10 | Закрытое акционерное общество "Молекулярно-медицинские технологии" | Способ изготовления многофункционального мультичипа, мультичип для последовательного или параллельного скрининга биополимеров, способ анализа биополимеров и набор для осуществления способа |
| CA2653321A1 (en) | 2006-05-26 | 2007-12-06 | Althea Technologies, Inc. | Biochemical analysis of partitioned cells |
| FR2901717A1 (fr) | 2006-05-30 | 2007-12-07 | Centre Nat Rech Scient | Procede de traitement de gouttes dans un circuit microfluidique. |
| AU2007260676A1 (en) | 2006-06-14 | 2007-12-21 | Artemis Health, Inc. | Rare cell analysis using sample splitting and DNA tags |
| US8715934B2 (en) | 2006-06-19 | 2014-05-06 | The Johns Hopkins University | Single-molecule PCR on microparticles in water-in-oil emulsions |
| US20080076909A1 (en) | 2006-06-30 | 2008-03-27 | Applera Corporation | Emulsion pcr and amplicon capture |
| EP1878501A1 (en) | 2006-07-14 | 2008-01-16 | Roche Diagnostics GmbH | Instrument for heating and cooling |
| US8394590B2 (en) | 2006-08-02 | 2013-03-12 | California Institute Of Technology | Capture agents and related methods and systems for detecting and/or sorting targets |
| EP3536396B1 (en) | 2006-08-07 | 2022-03-30 | The President and Fellows of Harvard College | Fluorocarbon emulsion stabilizing surfactants |
| US9278321B2 (en) | 2006-09-06 | 2016-03-08 | Canon U.S. Life Sciences, Inc. | Chip and cartridge design configuration for performing micro-fluidic assays |
| CN104774275A (zh) | 2006-09-25 | 2015-07-15 | 阿彻-丹尼尔斯-米德兰德公司 | 超吸收性的经表面处理的羧烷基化多糖及其制备方法 |
| US7935518B2 (en) | 2006-09-27 | 2011-05-03 | Alessandra Luchini | Smart hydrogel particles for biomarker harvesting |
| US20080166720A1 (en) | 2006-10-06 | 2008-07-10 | The Regents Of The University Of California | Method and apparatus for rapid nucleic acid analysis |
| US8841116B2 (en) | 2006-10-25 | 2014-09-23 | The Regents Of The University Of California | Inline-injection microdevice and microfabricated integrated DNA analysis system using same |
| WO2008070352A2 (en) | 2006-10-27 | 2008-06-12 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
| WO2008061165A2 (en) | 2006-11-14 | 2008-05-22 | Handylab, Inc. | Microfluidic cartridge and method of making same |
| US8603749B2 (en) | 2006-11-15 | 2013-12-10 | Biospherex, LLC | Multitag sequencing ecogenomics analysis-US |
| EP2109773A2 (en) | 2006-11-20 | 2009-10-21 | Nativis, Inc | Apparatus and method for transducing an in vitro or mammalian system with a low-frequency signal |
| US20080242560A1 (en) | 2006-11-21 | 2008-10-02 | Gunderson Kevin L | Methods for generating amplified nucleic acid arrays |
| US7866543B2 (en) | 2006-11-21 | 2011-01-11 | International Business Machines Corporation | Security and privacy enforcement for discovery services in a network of electronic product code information repositories |
| US8598328B2 (en) | 2006-12-13 | 2013-12-03 | National University Corporation Nagoya University | Tol1 factor transposase and DNA introduction system using the same |
| EP3285067B1 (en) | 2006-12-14 | 2022-06-22 | Life Technologies Corporation | Apparatus for measuring analytes using fet arrays |
| US8338166B2 (en) | 2007-01-04 | 2012-12-25 | Lawrence Livermore National Security, Llc | Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture |
| JP2008167722A (ja) | 2007-01-15 | 2008-07-24 | Konica Minolta Medical & Graphic Inc | 磁性支持体上での加熱による核酸単離方法 |
| US7844658B2 (en) | 2007-01-22 | 2010-11-30 | Comcast Cable Holdings, Llc | System and method for providing an application to a device |
| US20080176768A1 (en) | 2007-01-23 | 2008-07-24 | Honeywell Honeywell International | Hydrogel microarray with embedded metal nanoparticles |
| EP2121983A2 (en) | 2007-02-02 | 2009-11-25 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
| US8003312B2 (en) | 2007-02-16 | 2011-08-23 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplex cellular assays using detectable cell barcodes |
| FI20075124A0 (fi) | 2007-02-21 | 2007-02-21 | Valtion Teknillinen | Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi |
| US9029085B2 (en) | 2007-03-07 | 2015-05-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| US20080228268A1 (en) | 2007-03-15 | 2008-09-18 | Uluru, Inc. | Method of Formation of Viscous, Shape Conforming Gels and Their Uses as Medical Prosthesis |
| US7943330B2 (en) | 2007-03-23 | 2011-05-17 | Academia Sinica | Tailored glycoproteomic methods for the sequencing, mapping and identification of cellular glycoproteins |
| CN102014871A (zh) | 2007-03-28 | 2011-04-13 | 哈佛大学 | 乳液及其形成技术 |
| WO2008134153A1 (en) | 2007-04-23 | 2008-11-06 | Advanced Liquid Logic, Inc. | Bead-based multiplexed analytical methods and instrumentation |
| CN101293191B (zh) | 2007-04-25 | 2011-11-09 | 中国科学院过程工程研究所 | 一种琼脂糖凝胶微球的制备方法 |
| WO2008135512A2 (en) | 2007-05-02 | 2008-11-13 | Jerzy Paszkowski | Dna amplification method |
| US20080295909A1 (en) | 2007-05-24 | 2008-12-04 | Locascio Laurie E | Microfluidic Device for Passive Sorting and Storage of Liquid Plugs Using Capillary Force |
| CN101720359A (zh) | 2007-06-01 | 2010-06-02 | 454生命科学公司 | 从多重混合物中识别个别样本的系统和方法 |
| EP2162205A1 (en) | 2007-06-05 | 2010-03-17 | Eugenia Kumacheva | Multiple continuous microfluidic reactors for the scaled up synthesis of gel or polymer particles |
| US20100255556A1 (en) | 2007-06-29 | 2010-10-07 | President And Fellows Of Harvard College | Methods and apparatus for manipulation of fluidic species |
| US20090068170A1 (en) | 2007-07-13 | 2009-03-12 | President And Fellows Of Harvard College | Droplet-based selection |
| US8454906B2 (en) | 2007-07-24 | 2013-06-04 | The Regents Of The University Of California | Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions |
| US20130084243A1 (en) | 2010-01-27 | 2013-04-04 | Liliane Goetsch | Igf-1r specific antibodies useful in the detection and diagnosis of cellular proliferative disorders |
| CA2696843A1 (en) | 2007-08-15 | 2009-02-19 | Opgen, Inc. | Method, system and software arrangement for comparative analysis and phylogeny with whole-genome optical maps |
| US8563527B2 (en) | 2007-08-20 | 2013-10-22 | Pharmain Corporation | Oligonucleotide core carrier compositions for delivery of nucleic acid-containing therapeutic agents, methods of making and using the same |
| US8268564B2 (en) | 2007-09-26 | 2012-09-18 | President And Fellows Of Harvard College | Methods and applications for stitched DNA barcodes |
| WO2009048532A2 (en) | 2007-10-05 | 2009-04-16 | President And Fellows Of Harvard College | Formation of particles for ultrasound application, drug release, and other uses, and microfluidic methods of preparation |
| US20090099040A1 (en) | 2007-10-15 | 2009-04-16 | Sigma Aldrich Company | Degenerate oligonucleotides and their uses |
| US20100086914A1 (en) | 2008-10-03 | 2010-04-08 | Roche Molecular Systems, Inc. | High resolution, high throughput hla genotyping by clonal sequencing |
| EP2209912A1 (en) * | 2007-10-26 | 2010-07-28 | Rosetta Inpharmatics LLC | Cdna synthesis using non-random primers |
| WO2009061372A1 (en) | 2007-11-02 | 2009-05-14 | President And Fellows Of Harvard College | Systems and methods for creating multi-phase entities, including particles and/or fluids |
| US8334013B2 (en) | 2007-11-02 | 2012-12-18 | Stc.Unm | Mesoporous metal oxide microspheres and method for forming same |
| US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| CN101918590B (zh) | 2007-12-10 | 2013-03-27 | 高晓莲 | 核酸测序 |
| US7771944B2 (en) | 2007-12-14 | 2010-08-10 | The Board Of Trustees Of The University Of Illinois | Methods for determining genetic haplotypes and DNA mapping |
| EP2235210B1 (en) | 2007-12-21 | 2015-03-25 | President and Fellows of Harvard College | Methods for nucleic acid sequencing |
| EP2620511B1 (en) | 2008-01-17 | 2018-02-28 | Sequenom, Inc. | Single molecule nucleic acid sequence analysis processes |
| KR20090081260A (ko) | 2008-01-23 | 2009-07-28 | 삼성전자주식회사 | 마이크로 어레이 혼성화 검출 방법 |
| US8501922B2 (en) | 2008-02-07 | 2013-08-06 | Pacific Biosciences Of California, Inc. | CIS reactive oxygen quenchers integrated into linkers |
| JP5468271B2 (ja) | 2008-02-08 | 2014-04-09 | 花王株式会社 | 微粒子分散液の製造方法 |
| US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
| AU2009224170B2 (en) | 2008-03-11 | 2012-03-29 | National Cancer Center | Method for measuring chromosome, gene or specific nucleotide sequence copy numbers using SNP array |
| CN101241126A (zh) | 2008-03-14 | 2008-08-13 | 东华大学 | 一种生物条形码探针的制备方法 |
| US9011777B2 (en) | 2008-03-21 | 2015-04-21 | Lawrence Livermore National Security, Llc | Monodisperse microdroplet generation and stopping without coalescence |
| US8961902B2 (en) | 2008-04-23 | 2015-02-24 | Bioscale, Inc. | Method and apparatus for analyte processing |
| US9068181B2 (en) | 2008-05-23 | 2015-06-30 | The General Hospital Corporation | Microfluidic droplet encapsulation |
| DE102008025656B4 (de) | 2008-05-28 | 2016-07-28 | Genxpro Gmbh | Verfahren zur quantitativen Analyse von Nukleinsäuren, Marker dafür und deren Verwendung |
| GB0810051D0 (en) | 2008-06-02 | 2008-07-09 | Oxford Biodynamics Ltd | Method of diagnosis |
| WO2009148598A1 (en) | 2008-06-05 | 2009-12-10 | President And Fellows Of Harvard College | Polymersomes, colloidosomes, liposomes, and other species associated with fluidic droplets |
| US8198028B2 (en) | 2008-07-02 | 2012-06-12 | Illumina Cambridge Limited | Using populations of beads for the fabrication of arrays on surfaces |
| WO2010004018A2 (en) | 2008-07-11 | 2010-01-14 | Eth Zurich | Degradable microcapsules |
| EP2315629B1 (en) | 2008-07-18 | 2021-12-15 | Bio-Rad Laboratories, Inc. | Droplet libraries |
| US10722562B2 (en) | 2008-07-23 | 2020-07-28 | Immudex Aps | Combinatorial analysis and repair |
| US20100062494A1 (en) | 2008-08-08 | 2010-03-11 | President And Fellows Of Harvard College | Enzymatic oligonucleotide pre-adenylation |
| US8383345B2 (en) | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
| US20110218123A1 (en) | 2008-09-19 | 2011-09-08 | President And Fellows Of Harvard College | Creation of libraries of droplets and related species |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| US9822393B2 (en) | 2013-03-08 | 2017-11-21 | Bio-Rad Laboratories, Inc. | Compositions, methods and systems for polymerase chain reaction assays |
| US9417190B2 (en) | 2008-09-23 | 2016-08-16 | Bio-Rad Laboratories, Inc. | Calibrations and controls for droplet-based assays |
| US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
| US9764322B2 (en) | 2008-09-23 | 2017-09-19 | Bio-Rad Laboratories, Inc. | System for generating droplets with pressure monitoring |
| WO2011120024A1 (en) | 2010-03-25 | 2011-09-29 | Quantalife, Inc. | Droplet generation for droplet-based assays |
| US20120252015A1 (en) | 2011-02-18 | 2012-10-04 | Bio-Rad Laboratories | Methods and compositions for detecting genetic material |
| WO2013016459A1 (en) | 2011-07-25 | 2013-01-31 | Bio-Rad Laboratories, Inc. | Breakage of an emulsion containing nucleic acid |
| JP5670335B2 (ja) | 2008-09-25 | 2015-02-18 | セファロン、インク. | ベンダムスチン液体製剤 |
| US8361299B2 (en) | 2008-10-08 | 2013-01-29 | Sage Science, Inc. | Multichannel preparative electrophoresis system |
| DK2963709T3 (en) | 2008-10-24 | 2017-09-11 | Epicentre Tech Corp | TRANSPOSON-END COMPOSITIONS AND PROCEDURES FOR MODIFICATION OF NUCLEIC ACIDS |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| US20100113296A1 (en) | 2008-11-05 | 2010-05-06 | Joel Myerson | Methods And Kits For Nucleic Acid Analysis |
| US8748103B2 (en) | 2008-11-07 | 2014-06-10 | Sequenta, Inc. | Monitoring health and disease status using clonotype profiles |
| GB2483810B (en) * | 2008-11-07 | 2012-09-05 | Sequenta Inc | Methods for correlating clonotypes with diseases in a population |
| US20100203647A1 (en) | 2008-11-21 | 2010-08-12 | The Rockefeller University | Chemical Reporters of Protein Acylation |
| CN102292455A (zh) | 2008-12-02 | 2011-12-21 | 伯乐实验室公司 | 染色质结构检测 |
| EP3587594B1 (en) | 2008-12-19 | 2022-04-13 | President and Fellows of Harvard College | Particle-assisted nucleic acid sequencing |
| WO2010075570A2 (en) | 2008-12-24 | 2010-07-01 | New York University | Methods, computer-accessible medium, and systems for score-driven whole-genome shotgun sequence assemble |
| KR101065807B1 (ko) | 2009-01-23 | 2011-09-19 | 충남대학교산학협력단 | 액적 기반의 미세유체 칩을 이용한 마이크로 캡슐 제조방법 |
| US9347092B2 (en) | 2009-02-25 | 2016-05-24 | Roche Molecular System, Inc. | Solid support for high-throughput nucleic acid analysis |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ.ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| CN102405098A (zh) | 2009-03-13 | 2012-04-04 | 哈佛学院院长等 | 流动聚焦微流装置的尺度放大 |
| WO2010104604A1 (en) | 2009-03-13 | 2010-09-16 | President And Fellows Of Harvard College | Method for the controlled creation of emulsions, including multiple emulsions |
| EP2230312A1 (en) | 2009-03-19 | 2010-09-22 | Helmholtz-Zentrum für Infektionsforschung GmbH | Probe compound for detecting and isolating enzymes and means and methods using the same |
| EP3415235B1 (en) | 2009-03-23 | 2025-11-12 | Bio-Rad Laboratories, Inc. | Manipulation of microfluidic droplets |
| EP3002337B1 (en) | 2009-03-30 | 2018-10-24 | Illumina, Inc. | Gene expression analysis in single cells |
| EP2789694A1 (en) | 2009-04-02 | 2014-10-15 | Fluidigm Corporation | Microfluidic device with reaction product recovery system |
| US9085798B2 (en) | 2009-04-30 | 2015-07-21 | Prognosys Biosciences, Inc. | Nucleic acid constructs and methods of use |
| US20100279882A1 (en) | 2009-05-01 | 2010-11-04 | Mostafa Ronaghi | Sequencing methods |
| US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
| FR2945545B1 (fr) | 2009-05-14 | 2011-08-05 | Univ Aix Marseille Ii | Methode de detection d'adn procaryote extrait d'un echantillon de selles |
| FR2945819B1 (fr) | 2009-05-19 | 2011-06-17 | Commissariat Energie Atomique | Dispositif et procede d'isolement de cibles biologiques ou chimiques |
| US8574835B2 (en) | 2009-05-29 | 2013-11-05 | Life Technologies Corporation | Scaffolded nucleic acid polymer particles and methods of making and using |
| EP2443236B1 (en) | 2009-06-15 | 2015-05-13 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| SG177369A1 (en) | 2009-06-26 | 2012-02-28 | Harvard College | Fluid injection |
| US20110028412A1 (en) | 2009-08-03 | 2011-02-03 | Cappellos, Inc. | Herbal enhanced analgesic formulations |
| US20110033548A1 (en) | 2009-08-05 | 2011-02-10 | E.I. Du Pont De Nemours And Company | Degradable crosslinked aminated dextran microspheres and methods of use |
| WO2011021102A2 (en) | 2009-08-20 | 2011-02-24 | Population Genetics Technologies Ltd | Compositions and methods for intramolecular nucleic acid rearrangement |
| JP2013503605A (ja) | 2009-09-01 | 2013-02-04 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | マイクロアレイの選択のためのデバイス及び方法 |
| CA2767056C (en) | 2009-09-02 | 2018-12-04 | Bio-Rad Laboratories, Inc. | System for mixing fluids by coalescence of multiple emulsions |
| WO2011028764A2 (en) | 2009-09-02 | 2011-03-10 | President And Fellows Of Harvard College | Multiple emulsions created using jetting and other techniques |
| US9625454B2 (en) | 2009-09-04 | 2017-04-18 | The Research Foundation For The State University Of New York | Rapid and continuous analyte processing in droplet microfluidic devices |
| GB0918564D0 (en) | 2009-10-22 | 2009-12-09 | Plasticell Ltd | Nested cell encapsulation |
| WO2011056546A1 (en) | 2009-10-27 | 2011-05-12 | President And Fellows Of Harvard College | Droplet creation techniques |
| WO2011056872A2 (en) | 2009-11-03 | 2011-05-12 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
| EP2496700B1 (en) | 2009-11-04 | 2017-03-01 | The University Of British Columbia | Nucleic acid-containing lipid particles and related methods |
| CN102985552B (zh) | 2009-11-25 | 2016-02-17 | 伯乐生命医学产品有限公司 | 用于检测遗传物质的方法和组合物 |
| EP2504448B1 (en) | 2009-11-25 | 2016-10-19 | Bio-Rad Laboratories, Inc. | Methods and compositions for detecting genetic material |
| US9023769B2 (en) | 2009-11-30 | 2015-05-05 | Complete Genomics, Inc. | cDNA library for nucleic acid sequencing |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| AU2010330936B2 (en) | 2009-12-17 | 2014-05-22 | Keygene N.V. | Restriction enzyme based whole genome sequencing |
| EP2517025B1 (en) | 2009-12-23 | 2019-11-27 | Bio-Rad Laboratories, Inc. | Methods for reducing the exchange of molecules between droplets |
| CA2789425C (en) | 2010-02-12 | 2020-04-28 | Raindance Technologies, Inc. | Digital analyte analysis with polymerase error correction |
| JP5901046B2 (ja) | 2010-02-19 | 2016-04-06 | 国立大学法人 千葉大学 | OATP1B3mRNAの新規な選択的スプライシングバリアント |
| US20110257889A1 (en) | 2010-02-24 | 2011-10-20 | Pacific Biosciences Of California, Inc. | Sequence assembly and consensus sequence determination |
| EP2539450B1 (en) | 2010-02-25 | 2016-02-17 | Advanced Liquid Logic, Inc. | Method of making nucleic acid libraries |
| US8236574B2 (en) | 2010-03-01 | 2012-08-07 | Quanterix Corporation | Ultra-sensitive detection of molecules or particles using beads or other capture objects |
| US10500156B2 (en) | 2010-03-24 | 2019-12-10 | Northeastern University | Multi-compartmental macrophage delivery |
| FR2958186A1 (fr) | 2010-03-30 | 2011-10-07 | Ecole Polytech | Dispositif de formation de gouttes dans un circuit microfluide. |
| RS54482B1 (sr) | 2010-04-05 | 2016-06-30 | Prognosys Biosciences, Inc. | Biološka testiranja sa prostornim kodiranjem |
| US9255291B2 (en) | 2010-05-06 | 2016-02-09 | Bioo Scientific Corporation | Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing |
| US20110287947A1 (en) | 2010-05-18 | 2011-11-24 | University Of Southern California | Tethered Conformation Capture |
| CA2798323A1 (en) | 2010-05-26 | 2011-12-01 | Selecta Biosciences, Inc. | Dose selection of adjuvanted synthetic nanocarriers |
| US20120000777A1 (en) | 2010-06-04 | 2012-01-05 | The Regents Of The University Of California | Devices and methods for forming double emulsion droplet compositions and polymer particles |
| EP2580378A4 (en) | 2010-06-08 | 2014-01-01 | Nugen Technologies Inc | MULTIPLEX SEQUENCING METHODS AND COMPOSITION |
| US8703493B2 (en) | 2010-06-15 | 2014-04-22 | Src, Inc. | Location analysis using fire retardant-protected nucleic acid-labeled tags |
| WO2012003374A2 (en) | 2010-07-02 | 2012-01-05 | The Board Of Trustees Of The Leland Stanford Junior University | Targeted sequencing library preparation by genomic dna circularization |
| WO2012012037A1 (en) | 2010-07-19 | 2012-01-26 | New England Biolabs, Inc. | Oligonucleotide adaptors: compositions and methods of use |
| US20120034603A1 (en) | 2010-08-06 | 2012-02-09 | Tandem Diagnostics, Inc. | Ligation-based detection of genetic variants |
| CN103202812B (zh) | 2010-08-09 | 2015-10-28 | 南京大学 | 一种制备用于体内递送药理活性物质的蛋白纳米粒的方法 |
| WO2012019765A1 (en) | 2010-08-10 | 2012-02-16 | European Molecular Biology Laboratory (Embl) | Methods and systems for tracking samples and sample combinations |
| WO2012037358A1 (en) | 2010-09-16 | 2012-03-22 | The University Of North Carolina At Chapel Hill | Asymmetric bifunctional silyl monomers and particles thereof as prodrugs and delivery vehicles for pharmaceutical, chemical and biological agents |
| US8481292B2 (en) | 2010-09-21 | 2013-07-09 | Population Genetics Technologies Litd. | Increasing confidence of allele calls with molecular counting |
| CN102409048B (zh) | 2010-09-21 | 2013-10-23 | 深圳华大基因科技服务有限公司 | 一种基于高通量测序的dna标签文库构建方法 |
| WO2012045012A2 (en) * | 2010-09-30 | 2012-04-05 | Raindance Technologies, Inc. | Sandwich assays in droplets |
| KR101963462B1 (ko) | 2010-10-04 | 2019-03-28 | 제납시스 인크. | 재사용 가능한 자동화 평행 생물 반응 시스템 및 방법 |
| US9999886B2 (en) | 2010-10-07 | 2018-06-19 | The Regents Of The University Of California | Methods and systems for on demand droplet generation and impedance based detection |
| ES2966040T3 (es) * | 2010-10-08 | 2024-04-18 | Harvard College | Alto rendimiento de células individuales con código de barra |
| DK2625295T3 (da) | 2010-10-08 | 2019-06-11 | Harvard College | High-throughput-immunsekvensering |
| CN102050953B (zh) | 2010-10-18 | 2012-11-07 | 武汉理工大学 | 一种可还原降解超分子水凝胶的制备方法 |
| WO2012055929A1 (en) | 2010-10-26 | 2012-05-03 | Illumina, Inc. | Sequencing methods |
| US20130225623A1 (en) | 2010-10-27 | 2013-08-29 | Mount Sinai School Of Medicine | Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists |
| EP4016086A1 (en) | 2010-11-01 | 2022-06-22 | Bio-Rad Laboratories, Inc. | System for forming emulsions |
| CA2821299C (en) | 2010-11-05 | 2019-02-12 | Frank J. Steemers | Linking sequence reads using paired code tags |
| US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US8829171B2 (en) | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
| DK2652155T3 (en) | 2010-12-16 | 2017-02-13 | Gigagen Inc | Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells |
| CA2822439A1 (en) | 2010-12-23 | 2012-06-28 | Sequenom, Inc. | Fetal genetic variation detection |
| US9163281B2 (en) | 2010-12-23 | 2015-10-20 | Good Start Genetics, Inc. | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
| US20120191366A1 (en) | 2011-01-20 | 2012-07-26 | Nathaniel Pearson | Methods and Apparatus for Assigning a Meaningful Numeric Value to Genomic Variants, and Searching and Assessing Same |
| GB201101429D0 (en) | 2011-01-27 | 2011-03-16 | Biocompatibles Uk Ltd | Drug delivery system |
| US8765455B2 (en) | 2011-01-27 | 2014-07-01 | Lawrence Livermore National Security, Llc | Chip-based droplet sorting |
| US9238671B2 (en) | 2011-01-28 | 2016-01-19 | Illumina, Inc. | Oligonucleotide replacement for di-tagged and directional libraries |
| ES2687761T3 (es) | 2011-01-31 | 2018-10-29 | F. Hoffmann-La Roche Ag | Métodos de identificación de múltiples epítopos en células |
| CA2826131C (en) | 2011-02-02 | 2019-11-05 | Jay Ashok Shendure | Massively parallel continguity mapping |
| EP2673380B1 (en) | 2011-02-09 | 2018-12-12 | Bio-Rad Laboratories, Inc. | Analysis of nucleic acids |
| US9266104B2 (en) | 2011-02-11 | 2016-02-23 | Raindance Technologies, Inc. | Thermocycling device for nucleic acid amplification and methods of use |
| EP3859011A1 (en) | 2011-02-11 | 2021-08-04 | Bio-Rad Laboratories, Inc. | Methods for forming mixed droplets |
| EP3736281A1 (en) * | 2011-02-18 | 2020-11-11 | Bio-Rad Laboratories, Inc. | Compositions and methods for molecular labeling |
| WO2012116250A1 (en) | 2011-02-25 | 2012-08-30 | University Of Massachusetts Medical School | Monomers and polymers for functional polycarbonates and poly (ester-carbonates) and peg-co-polycarbonate hydrogels |
| CA2824431A1 (en) | 2011-02-25 | 2012-08-30 | Illumina, Inc. | Methods and systems for haplotype determination |
| WO2012121973A1 (en) | 2011-03-04 | 2012-09-13 | Life Technologies Corporation | Compounds and methods for conjugation of biomolecules |
| WO2012122548A2 (en) | 2011-03-09 | 2012-09-13 | Lawrence Ganeshalingam | Biological data networks and methods therefor |
| WO2012129187A1 (en) | 2011-03-18 | 2012-09-27 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays with combinatorial use of signals |
| US9260753B2 (en) | 2011-03-24 | 2016-02-16 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| WO2012150317A1 (en) | 2011-05-05 | 2012-11-08 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Linear dna amplification |
| GB2489714B (en) * | 2011-04-05 | 2013-11-06 | Tracesa Ltd | Fluid Identification Method |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| EP2754078A4 (en) | 2011-04-14 | 2015-12-02 | Complete Genomics Inc | PROCESSING AND ANALYSIS OF COMPLEX NUCLEIC ACID SEQUENCE DATA |
| AU2012249759A1 (en) | 2011-04-25 | 2013-11-07 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| US9957558B2 (en) | 2011-04-28 | 2018-05-01 | Life Technologies Corporation | Methods and compositions for multiplex PCR |
| SI2702146T1 (sl) | 2011-04-28 | 2019-06-28 | The Board Of Trustees Of The Leland Stanford Junior University | Identifikacija polinukleotidov, povezanih z vzorcem |
| JP6100685B2 (ja) | 2011-05-16 | 2017-03-22 | 地方独立行政法人 大阪府立病院機構 | 血中dnaの定量的検出による悪性新生物の病勢の進行を評価する方法 |
| CN103732256A (zh) | 2011-05-16 | 2014-04-16 | 皇家飞利浦有限公司 | 生物正交药物活化 |
| US9005935B2 (en) | 2011-05-23 | 2015-04-14 | Agilent Technologies, Inc. | Methods and compositions for DNA fragmentation and tagging by transposases |
| BR112013029729A2 (pt) | 2011-05-23 | 2017-01-24 | Basf Se | controle de emulsões, incluindo emulsões múltiplas |
| US9617598B2 (en) | 2011-05-27 | 2017-04-11 | President And Fellows Of Harvard College | Methods of amplifying whole genome of a single cell |
| DE202012013668U1 (de) | 2011-06-02 | 2019-04-18 | Raindance Technologies, Inc. | Enzymquantifizierung |
| US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
| US9150916B2 (en) | 2011-06-24 | 2015-10-06 | Beat Christen | Compositions and methods for identifying the essential genome of an organism |
| US8927218B2 (en) | 2011-06-27 | 2015-01-06 | Flir Systems, Inc. | Methods and compositions for segregating target nucleic acid from mixed nucleic acid samples |
| EP2729501A2 (en) | 2011-07-07 | 2014-05-14 | Life Technologies Corporation | Polymer particles, nucleic acid polymer particles and methods of making and using the same |
| US20130017978A1 (en) | 2011-07-11 | 2013-01-17 | Finnzymes Oy | Methods and transposon nucleic acids for generating a dna library |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
| US9605304B2 (en) | 2011-07-20 | 2017-03-28 | The Hong Kong Polytechnic University | Ultra-stable oligonucleotide-gold and-silver nanoparticle conjugates and method of their preparation |
| WO2013019751A1 (en) | 2011-07-29 | 2013-02-07 | Bio-Rad Laboratories, Inc., | Library characterization by digital assay |
| WO2013020089A2 (en) | 2011-08-04 | 2013-02-07 | Sage Science, Inc. | Systems and methods for processing fluids |
| US10364464B2 (en) | 2011-08-08 | 2019-07-30 | The Broad Institute, Inc. | Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence |
| US8846883B2 (en) | 2011-08-16 | 2014-09-30 | University Of Southhampton | Oligonucleotide ligation |
| WO2013035114A1 (en) | 2011-09-08 | 2013-03-14 | Decode Genetics Ehf | Tp53 genetic variants predictive of cancer |
| HK1199745A1 (en) | 2011-09-09 | 2015-07-17 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for obtaining a sequence |
| CN103945931B (zh) | 2011-09-26 | 2017-03-22 | 基因技术股份公司 | 高效的小体积核酸合成 |
| CN103958050B (zh) | 2011-09-28 | 2016-09-14 | 哈佛学院院长等 | 用于液滴产生和/或流体操纵的系统和方法 |
| WO2013055955A1 (en) | 2011-10-12 | 2013-04-18 | Complete Genomics, Inc. | Identification of dna fragments and structural variations |
| US9469874B2 (en) | 2011-10-18 | 2016-10-18 | The Regents Of The University Of California | Long-range barcode labeling-sequencing |
| US20130109576A1 (en) | 2011-10-28 | 2013-05-02 | Anthony P. Shuber | Methods for detecting mutations |
| EP2774277A4 (en) | 2011-11-04 | 2015-07-22 | Intel Corp | SIGNALING FOR THE CONFIGURATION OF COORDINATED DOWNLINK MULTIPORT COMMUNICATIONS |
| JP6083713B2 (ja) | 2011-11-16 | 2017-02-22 | インターナショナル・ビジネス・マシーンズ・コーポレーションInternational Business Machines Corporation | 変形可能バルブを備えたマイクロ流体デバイス |
| US9938524B2 (en) | 2011-11-22 | 2018-04-10 | Active Motif, Inc. | Multiplex isolation of protein-associated nucleic acids |
| US10689643B2 (en) | 2011-11-22 | 2020-06-23 | Active Motif, Inc. | Targeted transposition for use in epigenetic studies |
| ES2870857T3 (es) | 2011-12-03 | 2021-10-27 | Emd Millipore Corp | Sistema microfluídico para cultivo celular |
| EP4249605B1 (en) | 2011-12-22 | 2024-08-28 | President And Fellows Of Harvard College | Methods for analyte detection |
| WO2013096643A1 (en) | 2011-12-23 | 2013-06-27 | Gigagen | Methods and apparatuses for droplet mixing |
| CN104245745B (zh) | 2012-02-09 | 2017-03-29 | 生命技术公司 | 亲水性聚合物颗粒及其制备方法 |
| EP2814984A4 (en) | 2012-02-14 | 2015-07-29 | Univ Johns Hopkins | MIRNA ANALYSIS PROCEDURE |
| CA2864287C (en) | 2012-02-15 | 2021-01-12 | Wisconsin Alumni Research Foundation | Dithioamine reducing agents |
| DK3363901T3 (da) | 2012-02-17 | 2021-02-22 | Hutchinson Fred Cancer Res | Sammensætninger og fremgangsmåder til præcis identificering af mutationer |
| WO2013123125A1 (en) | 2012-02-17 | 2013-08-22 | President And Fellows Of Harvard College | Assembly of nucleic acid sequences in emulsions |
| US9176031B2 (en) | 2012-02-24 | 2015-11-03 | Raindance Technologies, Inc. | Labeling and sample preparation for sequencing |
| WO2013134261A1 (en) | 2012-03-05 | 2013-09-12 | President And Fellows Of Harvard College | Systems and methods for epigenetic sequencing |
| NO2694769T3 (cg-RX-API-DMAC7.html) | 2012-03-06 | 2018-03-03 | ||
| US9552458B2 (en) | 2012-03-16 | 2017-01-24 | The Research Institute At Nationwide Children's Hospital | Comprehensive analysis pipeline for discovery of human genetic variation |
| US20150125904A1 (en) | 2012-03-30 | 2015-05-07 | Massachusetts Institute Of Technology | Probe incorporation mediated by enzymes |
| CN102622634B (zh) | 2012-03-31 | 2014-04-23 | 中国农业科学院果树研究所 | 一种苹果种质资源条形码标识的制备方法 |
| EP2647426A1 (en) | 2012-04-03 | 2013-10-09 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding |
| US8209130B1 (en) | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
| WO2013158686A1 (en) | 2012-04-16 | 2013-10-24 | Biological Dynamics, Inc. | Nucleic acid sample preparation |
| US20130296173A1 (en) | 2012-04-23 | 2013-11-07 | Complete Genomics, Inc. | Pre-anchor wash |
| WO2013177046A1 (en) | 2012-05-21 | 2013-11-28 | Solulink, Inc. | Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization |
| EP3514243B1 (en) | 2012-05-21 | 2022-08-17 | The Scripps Research Institute | Methods of sample preparation |
| JP2015519909A (ja) | 2012-06-15 | 2015-07-16 | アダプティブ バイオテクノロジーズ コーポレイション | 複合遺伝子セットにおける固有にタグ付加された再構成適応性免疫受容体遺伝子 |
| CN104619862B (zh) * | 2012-06-15 | 2017-10-31 | 得克萨斯系统大学董事会 | 单细胞的多转录物的高通量测序 |
| SG11201500313YA (en) | 2012-07-24 | 2015-02-27 | Sequenta Inc | Single cell analysis using sequence tags |
| CN105264088B (zh) | 2012-08-08 | 2018-12-28 | 豪夫迈·罗氏有限公司 | 提高鉴定细胞中的多个表位的动态范围 |
| US10161007B2 (en) | 2012-08-13 | 2018-12-25 | The Regents Of The University Of California | Methods and systems for detecting biological components |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US20220098659A1 (en) | 2012-08-14 | 2022-03-31 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US20150005199A1 (en) | 2012-08-14 | 2015-01-01 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US20140378322A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| BR112015003354A8 (pt) | 2012-08-14 | 2018-01-16 | 10X Genomics Inc | métodos e composições de microcápsula |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US20150005200A1 (en) | 2012-08-14 | 2015-01-01 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US20140378345A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US20140378349A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| WO2014047561A1 (en) | 2012-09-21 | 2014-03-27 | The Broad Institute Inc. | Compositions and methods for labeling of agents |
| US9644199B2 (en) | 2012-10-01 | 2017-05-09 | Agilent Technologies, Inc. | Immobilized transposase complexes for DNA fragmentation and tagging |
| GB201217772D0 (en) | 2012-10-04 | 2012-11-14 | Base4 Innovation Ltd | Sequencing method |
| FR2996544B1 (fr) | 2012-10-08 | 2015-03-13 | Ecole Polytech | Circuit microfluidique permettant la mise en contact de gouttes de plusieurs fluides, et procede microfluidique correspondant. |
| FR2996545B1 (fr) | 2012-10-08 | 2016-03-25 | Ecole Polytech | Procede microfluidique de traitement et d'analyse d'une solution contenant un materiel biologique, et circuit microfluidique correspondant. |
| EP3252174B1 (en) | 2012-10-15 | 2020-07-01 | Life Technologies Corporation | Compositions, methods, systems and kits for target nucleic acid enrichment |
| EP3901280B1 (en) | 2012-10-17 | 2025-03-12 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| JP6454281B2 (ja) | 2012-11-05 | 2019-01-16 | タカラ バイオ ユーエスエー, インコーポレイテッド | バーコード化する核酸 |
| EP2917366B1 (en) | 2012-11-06 | 2017-08-02 | Oxford Nanopore Technologies Limited | Quadruplex method |
| WO2014074611A1 (en) | 2012-11-07 | 2014-05-15 | Good Start Genetics, Inc. | Methods and systems for identifying contamination in samples |
| US20150299772A1 (en) | 2012-12-03 | 2015-10-22 | Elim Biopharmaceuticals, Inc. | Single-stranded polynucleotide amplification methods |
| PL2771468T3 (pl) | 2012-12-12 | 2015-07-31 | Broad Inst Inc | Inżynieria systemów, metod i zoptymalizowanych kompozycji kierujących dla manipulacji sekwencjami |
| EP2931899A1 (en) | 2012-12-12 | 2015-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| EP3567116A1 (en) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP2749653A1 (en) | 2012-12-28 | 2014-07-02 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Molecular coding for analysis of composition of macromolecules and molecular complexes |
| EP2752664A1 (en) | 2013-01-07 | 2014-07-09 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Label-free method for the detection of analytes |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| WO2014113736A1 (en) | 2013-01-17 | 2014-07-24 | Edico Genome Corp. | Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform |
| KR101984699B1 (ko) | 2013-01-24 | 2019-05-31 | 삼성전자주식회사 | 핵산 분석용 미세 유체 시스템 |
| WO2014117088A1 (en) | 2013-01-25 | 2014-07-31 | Gnubio, Inc. | System and method for performing droplet inflation |
| US10381106B2 (en) | 2013-01-28 | 2019-08-13 | Hasso-Plattner-Institut Fuer Softwaresystemtechnik Gmbh | Efficient genomic read alignment in an in-memory database |
| WO2014121241A1 (en) | 2013-02-01 | 2014-08-07 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
| CA2900481A1 (en) | 2013-02-08 | 2014-08-14 | 10X Genomics, Inc. | Polynucleotide barcode generation |
| EP2964390B1 (en) | 2013-03-06 | 2018-12-26 | President and Fellows of Harvard College | Use of devices for forming relatively monodisperse droplets |
| EP2970951B1 (en) | 2013-03-13 | 2019-02-20 | Illumina, Inc. | Methods for nucleic acid sequencing |
| WO2014152155A1 (en) | 2013-03-14 | 2014-09-25 | The Broad Institute, Inc. | Massively multiplexed rna sequencing |
| US9273349B2 (en) | 2013-03-14 | 2016-03-01 | Affymetrix, Inc. | Detection of nucleic acids |
| CA2906818C (en) | 2013-03-15 | 2022-08-16 | Verinata Health, Inc. | Generating cell-free dna libraries directly from blood |
| CN105358984B (zh) | 2013-03-15 | 2020-02-18 | 普罗格诺西斯生物科学公司 | 用于检测肽/mhc/tcr结合的方法 |
| US9328382B2 (en) | 2013-03-15 | 2016-05-03 | Complete Genomics, Inc. | Multiple tagging of individual long DNA fragments |
| WO2014144495A1 (en) | 2013-03-15 | 2014-09-18 | Abvitro, Inc. | Single cell bar-coding for antibody discovery |
| US20140272996A1 (en) | 2013-03-15 | 2014-09-18 | Bio-Rad Laboratories, Inc. | Droplet generator with collection tube |
| US20140274729A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Methods, compositions and kits for generation of stranded rna or dna libraries |
| DK2971080T3 (en) | 2013-03-15 | 2018-02-12 | Expedeon S L | METHODS FOR AMPLIFICATION AND SEQUENCE USING THERMOSTABLE TTHPRIMPOL |
| EP4566715A3 (en) | 2013-04-02 | 2025-08-27 | Bio-Rad Laboratories, Inc. | Systems and methods for handling microfluidic droplets |
| CN105431575B (zh) | 2013-05-09 | 2017-08-29 | 生物辐射实验室股份有限公司 | 磁性免疫数字pcr试验 |
| EP3828285B1 (en) | 2013-05-23 | 2023-12-13 | The Board of Trustees of the Leland Stanford Junior University | Transposition into native chromatin for personal epigenomics |
| EP3008201B1 (en) | 2013-06-12 | 2019-08-07 | The General Hospital Corporation | Methods for multiplexed detection of target molecules and uses thereof |
| US20160122753A1 (en) | 2013-06-12 | 2016-05-05 | Tarjei Mikkelsen | High-throughput rna-seq |
| AU2014281028B2 (en) | 2013-06-17 | 2020-09-10 | Massachusetts Institute Of Technology | Delivery and use of the CRISPR-Cas systems, vectors and compositions for hepatic targeting and therapy |
| US20160208323A1 (en) | 2013-06-21 | 2016-07-21 | The Broad Institute, Inc. | Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing |
| LT3013983T (lt) | 2013-06-25 | 2023-05-10 | Prognosys Biosciences, Inc. | Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį |
| BR112015032512A8 (pt) * | 2013-06-27 | 2020-01-07 | 10X Genomics Inc | método de implementação de código de barras de materiais de amostra |
| US9840718B2 (en) | 2013-07-12 | 2017-12-12 | University Of South Alabama | Minimal piggyBac vectors for genome integration |
| CN103394410B (zh) | 2013-07-25 | 2016-04-20 | 博奥生物集团有限公司 | 一种位置可调节的智能磁力架 |
| GB2516684A (en) | 2013-07-30 | 2015-02-04 | Sphere Fluidics Ltd | Microfluidic devices and systems |
| EP3039158B1 (en) | 2013-08-28 | 2018-11-14 | Cellular Research, Inc. | Massively parallel single cell analysis |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| US9381217B2 (en) * | 2013-09-09 | 2016-07-05 | Georgia Tech Research Corporation | Microgels for encapsulation of cells and other biologic agents |
| EP3049067A4 (en) | 2013-09-24 | 2017-05-03 | The Regents of the University of California | Encapsulated sensors and sensing systems for bioassays and diagnostics and methods for making and using them |
| GB201317301D0 (en) | 2013-09-30 | 2013-11-13 | Linnarsson Sten | Method for capturing and encoding nucleic acid from a plurality of single cells |
| CA2926934A1 (en) | 2013-10-09 | 2015-04-16 | Stc.Unm | Synthetic long read dna sequencing |
| US9937495B2 (en) | 2013-10-28 | 2018-04-10 | Massachusetts Institute Of Technology | Hydrogel microstructures with immiscible fluid isolation for small reaction volumes |
| US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
| US20140315755A1 (en) | 2013-12-26 | 2014-10-23 | Tao Chen | Genome-wide Antisense Oligonucleotide and RNAi |
| CN106460033B (zh) | 2013-12-30 | 2021-12-24 | 阿特雷卡公司 | 使用核酸条形码分析与单细胞缔合的核酸 |
| KR101464100B1 (ko) | 2014-01-29 | 2014-11-21 | 성균관대학교산학협력단 | 생체 적용 가능한 리포솜-핵산형광 나노 융합체, 이의 응용, 및 이의 제조방법 |
| US20170009288A1 (en) | 2014-02-03 | 2017-01-12 | Thermo Fisher Scientific Baltics Uab | Method for controlled dna fragmentation |
| US9822396B2 (en) | 2014-02-13 | 2017-11-21 | Bio-Rad Laboratories, Inc. | Chromosome conformation capture in partitions |
| ES2894048T3 (es) | 2014-02-27 | 2022-02-11 | Jumpcode Genomics Inc | Procedimientos para el análisis de elementos móviles somáticos y usos de los mismos |
| KR102596508B1 (ko) | 2014-04-10 | 2023-10-30 | 10엑스 제노믹스, 인크. | 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용 |
| EP3845640A3 (en) | 2014-04-15 | 2021-09-01 | Illumina, Inc. | Modified tranposases for improved insertion sequence bias and increased dna input tolerence |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| CN107075543B (zh) | 2014-04-21 | 2021-11-16 | 哈佛学院院长及董事 | 用于条形码化核酸的系统和方法 |
| US10975371B2 (en) | 2014-04-29 | 2021-04-13 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
| EP3146046B1 (en) | 2014-05-23 | 2020-03-11 | Digenomix Corporation | Haploidome determination by digitized transposons |
| EP3628684B1 (en) | 2014-06-06 | 2024-09-18 | Herlev Hospital | Determining antigen recognition through barcoding of mhc multimers |
| JP6666268B2 (ja) | 2014-06-11 | 2020-03-13 | サンプリクス アンパーツゼルスカブ | 液滴ソートによるヌクレオチド配列排除富化(needls) |
| US9534215B2 (en) | 2014-06-11 | 2017-01-03 | Life Technologies Corporation | Systems and methods for substrate enrichment |
| EP3155426B1 (en) | 2014-06-13 | 2023-07-19 | Immudex ApS | General detection and isolation of specific cells by binding of labeled molecules |
| US10480021B2 (en) | 2014-06-23 | 2019-11-19 | Yale University | Methods for closed chromatin mapping and DNA methylation analysis for single cells |
| WO2015200541A1 (en) | 2014-06-24 | 2015-12-30 | Bio-Rad Laboratories, Inc. | Digital pcr barcoding |
| MX2016016898A (es) | 2014-06-26 | 2017-04-25 | 10X Genomics Inc | Metodos y composiciones para analisis de muestras. |
| EP4574974A3 (en) | 2014-06-26 | 2025-10-08 | 10x Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| US10017759B2 (en) | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
| AU2015279546B2 (en) | 2014-06-26 | 2021-04-08 | 10X Genomics, Inc. | Processes and systems for nucleic acid sequence assembly |
| KR20170026383A (ko) | 2014-06-26 | 2017-03-08 | 10엑스 제노믹스, 인크. | 핵산 서열의 분석 |
| US10119167B2 (en) | 2014-07-18 | 2018-11-06 | Illumina, Inc. | Non-invasive prenatal diagnosis of fetal genetic condition using cellular DNA and cell free DNA |
| US20160024558A1 (en) | 2014-07-23 | 2016-01-28 | 10X Genomics, Inc. | Nucleic acid binding proteins and uses thereof |
| CN105335116B (zh) | 2014-07-30 | 2018-11-09 | 联想(北京)有限公司 | 一种显示控制方法及电子设备 |
| EP3186418A2 (en) | 2014-08-26 | 2017-07-05 | Nugen Technologies, Inc. | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
| EP3191605B1 (en) | 2014-09-09 | 2022-07-27 | The Broad Institute, Inc. | A droplet-based method and apparatus for composite single-cell nucleic acid analysis |
| SG10201911069WA (en) | 2014-09-15 | 2020-01-30 | Abvitro Llc | High-throughput nucleotide library sequencing |
| SG10201903408VA (en) | 2014-10-17 | 2019-05-30 | Illumina Cambridge Ltd | Contiguity preserving transposition |
| US20160122817A1 (en) | 2014-10-29 | 2016-05-05 | 10X Genomics, Inc. | Methods and compositions for targeted nucleic acid sequencing |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| JP2017537645A (ja) | 2014-12-19 | 2017-12-21 | アプライズ バイオ, インコーポレイテッド | 細胞の選択された部分集団中の複数エピトープを識別するための方法 |
| SG11201705615UA (en) | 2015-01-12 | 2017-08-30 | 10X Genomics Inc | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
| EP3245605B1 (en) | 2015-01-13 | 2022-04-20 | 10X Genomics, Inc. | Systems and methods for visualizing structural variation and phasing information |
| CA2974306A1 (en) | 2015-02-04 | 2016-08-11 | The Regents Of The University Of California | Sequencing of nucleic acids via barcoding in discrete entities |
| CA2975852A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| JP2018513445A (ja) | 2015-02-09 | 2018-05-24 | 10エックス ゲノミクス,インコーポレイテッド | 構造変異の特定及びバリアントコールデータを用いたフェージングのためのシステム及び方法 |
| DK3256604T3 (da) | 2015-02-10 | 2020-05-25 | Illumina Inc | Fremgangsmåder og sammensætninger til analyse af cellebestanddele |
| US10697000B2 (en) | 2015-02-24 | 2020-06-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| CN107532202A (zh) | 2015-02-24 | 2018-01-02 | 10X 基因组学有限公司 | 用于靶向核酸序列覆盖的方法 |
| US9727810B2 (en) | 2015-02-27 | 2017-08-08 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| WO2016145409A1 (en) | 2015-03-11 | 2016-09-15 | The Broad Institute, Inc. | Genotype and phenotype coupling |
| WO2016149661A1 (en) | 2015-03-18 | 2016-09-22 | The Broad Institute, Inc. | Massively parallel on-chip coalescence of microemulsions |
| US20160289769A1 (en) | 2015-03-30 | 2016-10-06 | Verily Life Sciences Llc | Methods for Combining Single Cell Profiling with Combinatorial Nanoparticle Conjugate Library Screening and In Vivo Diagnostic System |
| HK1250245A1 (zh) | 2015-04-10 | 2018-12-07 | Spatial Transcriptomics Ab | 生物样本的空间区别、多重核酸分析 |
| WO2016168584A1 (en) | 2015-04-17 | 2016-10-20 | President And Fellows Of Harvard College | Barcoding systems and methods for gene sequencing and other applications |
| WO2016169431A1 (zh) | 2015-04-20 | 2016-10-27 | 深圳华大基因研究院 | 一种长片段dna文库构建方法 |
| EP3708256B1 (en) | 2015-04-22 | 2022-11-30 | Stilla Technologies | Contact-less priming method for loading a solution in a microfluidic device and associated system |
| US20160314242A1 (en) | 2015-04-23 | 2016-10-27 | 10X Genomics, Inc. | Sample indexing methods and compositions for sequencing applications |
| US20170016041A1 (en) | 2015-05-18 | 2017-01-19 | 10X Genomics, Inc. | Stabilized reducing agents and methods using same |
| WO2016187256A2 (en) | 2015-05-18 | 2016-11-24 | 10X Genomics, Inc. | Mobile solid phase compositions for use in biochemical reactions and analyses |
| EP3304383B1 (en) | 2015-05-26 | 2021-07-07 | Pacific Biosciences of California, Inc. | De novo diploid genome assembly and haplotype sequence reconstruction |
| WO2016187717A1 (en) | 2015-05-26 | 2016-12-01 | Exerkine Corporation | Exosomes useful for genome editing |
| WO2016191618A1 (en) | 2015-05-27 | 2016-12-01 | Jianbiao Zheng | Methods of inserting molecular barcodes |
| AU2016281766B2 (en) | 2015-06-24 | 2019-05-09 | Oxford BioDynamics PLC | Epigenetic chromosome interactions |
| RU2018105835A (ru) | 2015-07-17 | 2019-08-19 | Президент Энд Фэллоуз Оф Харвард Коллидж | Способы амплификации последовательностей нуклеиновых кислот |
| LT3334841T (lt) | 2015-08-12 | 2020-02-10 | Cemm - Forschungszentrum Für Molekulare Medizin Gmbh | Nukleorūgščių tyrimo būdai |
| WO2017034970A1 (en) | 2015-08-21 | 2017-03-02 | The General Hospital Corporation | Combinatorial single molecule analysis of chromatin |
| JP6869233B2 (ja) | 2015-09-24 | 2021-05-12 | アブビトロ, エルエルシー | 親和性−オリゴヌクレオチドコンジュゲートおよびその使用 |
| CN108289797B (zh) | 2015-10-13 | 2022-01-28 | 哈佛学院院长及董事 | 用于制备和使用凝胶微球的系统和方法 |
| EP3366818B1 (en) | 2015-10-19 | 2021-04-14 | Zhejiang Annoroad Bio-Technology Co., Ltd. | Method for constructing high-resolution single cell hi-c library with a lot of information |
| EP3365454A1 (en) | 2015-10-20 | 2018-08-29 | 10X Genomics, Inc. | Methods and systems for high throughput single cell genetic manipulation |
| WO2017075265A1 (en) | 2015-10-28 | 2017-05-04 | The Broad Institute, Inc. | Multiplex analysis of single cell constituents |
| WO2017075294A1 (en) | 2015-10-28 | 2017-05-04 | The Board Institute Inc. | Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction |
| WO2017079593A1 (en) | 2015-11-04 | 2017-05-11 | Atreca, Inc. | Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells |
| EP3377657B1 (en) | 2015-11-19 | 2020-11-11 | 10X Genomics, Inc. | Transformable tagging methods |
| EP4144861B1 (en) | 2015-12-04 | 2024-09-11 | 10X Genomics, Inc. | Methods and compositions for nucleic acid analysis |
| EP3397764A4 (en) | 2015-12-30 | 2019-05-22 | Bio-Rad Laboratories, Inc. | DIGITAL PROTEIN QUANTIFICATION |
| US20170260584A1 (en) | 2016-02-11 | 2017-09-14 | 10X Genomics, Inc. | Cell population analysis using single nucleotide polymorphisms from single cell transcriptomes |
| JP6735348B2 (ja) | 2016-02-11 | 2020-08-05 | 10エックス ジェノミクス, インコーポレイテッド | 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体 |
| EP3423598B1 (en) | 2016-03-01 | 2024-05-01 | Universal Sequencing Technology Corporation | Methods and kits for tracking nucleic acid target origin for nucleic acid sequencing |
| EP3868879A1 (en) | 2016-03-10 | 2021-08-25 | The Board of Trustees of the Leland Stanford Junior University | Transposase-mediated imaging of the accessible genome |
| CN109312402A (zh) | 2016-04-11 | 2019-02-05 | 得克萨斯州大学系统董事会 | 用于检测单个t细胞受体亲和力和序列的方法和组合物 |
| CN109311010B (zh) | 2016-04-15 | 2022-05-17 | 哈佛学院院长及董事 | 用于收集液滴或其他实体的系统和方法 |
| US20190127789A1 (en) | 2016-04-19 | 2019-05-02 | President And Fellows Of Harvard College | Immobilization-based systems and methods for genetic analysis and other applications |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| CN109804068A (zh) | 2016-08-10 | 2019-05-24 | 哈佛学院董事及会员团体 | 从头组装条码化的基因组dna片段的方法 |
| WO2018039338A1 (en) | 2016-08-23 | 2018-03-01 | 10X Genomics, Inc. | Microfluidic surface-mediated emulsion stability control |
| US10858699B2 (en) | 2016-08-30 | 2020-12-08 | Integrated Dna Technologies, Inc. | Cleavable hairpin primers |
| WO2018045186A1 (en) | 2016-08-31 | 2018-03-08 | President And Fellows Of Harvard College | Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing |
| EP3507379A4 (en) | 2016-08-31 | 2020-05-13 | President and Fellows of Harvard College | METHOD FOR DIGITAL TOTAL GENOM AMPLIFICATION |
| US20180080021A1 (en) | 2016-09-17 | 2018-03-22 | The Board Of Trustees Of The Leland Stanford Junior University | Simultaneous sequencing of rna and dna from the same sample |
| EP3516400B1 (en) | 2016-09-26 | 2023-08-16 | Becton, Dickinson and Company | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| CN114875125A (zh) | 2016-10-19 | 2022-08-09 | 10X基因组学有限公司 | 用于条形码化单个细胞或细胞群的核酸分子的方法和系统 |
| GB201619458D0 (en) | 2016-11-17 | 2017-01-04 | Spatial Transcriptomics Ab | Method for spatial tagging and analysing nucleic acids in a biological specimen |
| DK3553180T3 (da) | 2016-12-07 | 2022-07-25 | Mgi Tech Co Ltd | Fremgangsmåde til konstruktion af enkeltcellesekventeringsbibliotek og anvendelse deraf |
| CN110139932B (zh) | 2016-12-19 | 2024-05-17 | 生物辐射实验室股份有限公司 | 液滴加标的相邻性保留的标签化dna |
| AU2017382905A1 (en) | 2016-12-21 | 2019-07-04 | The Regents Of The University Of California | Single cell genomic sequencing using hydrogel based droplets |
| US20190177800A1 (en) | 2017-12-08 | 2019-06-13 | 10X Genomics, Inc. | Methods and compositions for labeling cells |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| WO2018125982A1 (en) | 2016-12-29 | 2018-07-05 | Illumina, Inc. | Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments |
| JP7229923B2 (ja) | 2017-01-06 | 2023-02-28 | エディタス・メディシン、インコーポレイテッド | ヌクレアーゼ切断を評価する方法 |
| WO2018132635A1 (en) | 2017-01-12 | 2018-07-19 | Massachusetts Institute Of Technology | Methods for analyzing t cell receptors and b cell receptors |
| EP4310183B1 (en) | 2017-01-30 | 2025-07-09 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| DK3583214T5 (da) * | 2017-02-02 | 2024-09-02 | New York Genome Center Inc | Fremgangsmåder og sammensætninger til identificering eller kvantificering af mål i en biologisk prøve |
| US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| US10347365B2 (en) | 2017-02-08 | 2019-07-09 | 10X Genomics, Inc. | Systems and methods for visualizing a pattern in a dataset |
| GB201704402D0 (en) | 2017-03-20 | 2017-05-03 | Blacktrace Holdings Ltd | Single cell DNA sequencing |
| WO2018174827A1 (en) | 2017-03-24 | 2018-09-27 | National University Of Singapore | Methods for multiplex detection of molecules |
| US10697008B2 (en) | 2017-04-12 | 2020-06-30 | Karius, Inc. | Sample preparation methods, systems and compositions |
| US12054764B2 (en) | 2017-04-14 | 2024-08-06 | The Broad Institute, Inc. | High-throughput screens for exploring biological functions of microscale biological systems |
| US20180312822A1 (en) | 2017-04-26 | 2018-11-01 | 10X Genomics, Inc. | Mmlv reverse transcriptase variants |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| CN110945139B (zh) | 2017-05-18 | 2023-09-05 | 10X基因组学有限公司 | 用于分选液滴和珠的方法和系统 |
| CN110870018B (zh) | 2017-05-19 | 2024-11-22 | 10X基因组学有限公司 | 用于分析数据集的系统和方法 |
| US10914729B2 (en) | 2017-05-22 | 2021-02-09 | The Trustees Of Princeton University | Methods for detecting protein binding sequences and tagging nucleic acids |
| US20180340169A1 (en) | 2017-05-26 | 2018-11-29 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| JP7536450B2 (ja) | 2017-06-05 | 2024-08-20 | ベクトン・ディキンソン・アンド・カンパニー | 単一細胞用のサンプルインデックス付加 |
| WO2018226546A1 (en) | 2017-06-05 | 2018-12-13 | 10X Genomics, Inc. | Gaskets for the distribution of pressures in a microfluidic system |
| WO2018236615A1 (en) | 2017-06-20 | 2018-12-27 | 10X Genomics, Inc. | Methods and systems for improved droplet stabilization |
| CA3068273A1 (en) | 2017-06-21 | 2018-12-27 | Bluedot Llc | Systems and methods for identification of nucleic acids in a sample |
| CN108336542B (zh) | 2017-06-23 | 2020-02-21 | 番禺得意精密电子工业有限公司 | 电连接器 |
| KR102694654B1 (ko) | 2017-08-01 | 2024-08-12 | 일루미나, 인코포레이티드 | 뉴클레오티드 서열분석용 하이드로겔 비드 |
| US9946577B1 (en) | 2017-08-14 | 2018-04-17 | 10X Genomics, Inc. | Systems and methods for distributed resource management |
| US10357771B2 (en) | 2017-08-22 | 2019-07-23 | 10X Genomics, Inc. | Method of producing emulsions |
| US10590244B2 (en) | 2017-10-04 | 2020-03-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| EP3691703A1 (en) | 2017-10-04 | 2020-08-12 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| US10837047B2 (en) | 2017-10-04 | 2020-11-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS |
| WO2019084328A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS FOR PREPARING NUCLEIC ACID MOLECULES |
| US20190127731A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | Methods for preparing nucleic acid molecules |
| CN114525273B (zh) | 2017-10-27 | 2025-01-28 | 10X基因组学有限公司 | 用于样品制备和分析的方法和系统 |
| WO2019099751A1 (en) | 2017-11-15 | 2019-05-23 | 10X Genomics, Inc. | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| WO2019108851A1 (en) | 2017-11-30 | 2019-06-06 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation and analysis |
| WO2019113235A1 (en) | 2017-12-06 | 2019-06-13 | 10X Genomics, Inc. | Methods and systems for processing nucleic acid molecules |
| WO2019118355A1 (en) | 2017-12-12 | 2019-06-20 | 10X Genomics, Inc. | Systems and methods for single cell processing |
| CN118547046A (zh) | 2017-12-22 | 2024-08-27 | 10X基因组学有限公司 | 用于处理来自一个或多个细胞的核酸分子的系统和方法 |
| WO2019148042A1 (en) | 2018-01-26 | 2019-08-01 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| EP4299755A3 (en) | 2018-02-05 | 2024-04-10 | The Board of Trustees of the Leland Stanford Junior University | Systems and methods for multiplexed measurements in single and ensemble cells |
| CN112005115A (zh) | 2018-02-12 | 2020-11-27 | 10X基因组学有限公司 | 表征来自单个细胞或细胞群体的多种分析物的方法 |
| SG11202008080RA (en) | 2018-02-22 | 2020-09-29 | 10X Genomics Inc | Ligation mediated analysis of nucleic acids |
| WO2019169028A1 (en) | 2018-02-28 | 2019-09-06 | 10X Genomics, Inc. | Transcriptome sequencing through random ligation |
| WO2019169347A1 (en) | 2018-03-02 | 2019-09-06 | 10X Genomics, Inc. | Systems and apparatus for holding plates |
| WO2019191321A1 (en) | 2018-03-28 | 2019-10-03 | 10X Genomics, Inc. | Nucleic acid enrichment within partitions |
| SG11202009889VA (en) | 2018-04-06 | 2020-11-27 | 10X Genomics Inc | Systems and methods for quality control in single cell processing |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| US20190345636A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| US20190352717A1 (en) | 2018-05-18 | 2019-11-21 | 10X Genomics, Inc. | Targeted non-invasive prenatal testing |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| US11703427B2 (en) | 2018-06-25 | 2023-07-18 | 10X Genomics, Inc. | Methods and systems for cell and bead processing |
| WO2020006183A1 (en) | 2018-06-28 | 2020-01-02 | 10X Genomics, Inc. | Systems and methods for visualization of single-cell resolution characteristics |
| US20200033366A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| EP3830289A1 (en) | 2018-08-03 | 2021-06-09 | 10X Genomics, Inc. | Methods and systems to minimize barcode exchange |
| US12065688B2 (en) | 2018-08-20 | 2024-08-20 | 10X Genomics, Inc. | Compositions and methods for cellular processing |
| WO2020041148A1 (en) | 2018-08-20 | 2020-02-27 | 10X Genomics, Inc. | Methods and systems for detection of protein-dna interactions using proximity ligation |
| US20200105373A1 (en) | 2018-09-28 | 2020-04-02 | 10X Genomics, Inc. | Systems and methods for cellular analysis using nucleic acid sequencing |
| US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
| CN113874521B (zh) | 2019-01-06 | 2024-11-01 | 10X基因组学有限公司 | 用于富集条形码的方法和系统 |
| US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| WO2020168013A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| WO2020167862A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transfer of reagents between droplets |
| WO2020167866A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transposon loading |
| US11920183B2 (en) | 2019-03-11 | 2024-03-05 | 10X Genomics, Inc. | Systems and methods for processing optically tagged beads |
| EP3947722A1 (en) | 2019-03-27 | 2022-02-09 | 10X Genomics, Inc. | Systems and methods for processing rna from cells |
| CN114729392A (zh) | 2019-09-06 | 2022-07-08 | 10X基因组学有限公司 | 用于对细胞和细胞珠粒进行条形码化的系统和方法 |
| EP4081777A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Reversible fixing reagents and methods of use thereof |
| US12241890B2 (en) | 2019-12-23 | 2025-03-04 | 10X Genomics, Inc. | Methods for generating barcoded nucleic acid molecules using fixed cells |
| CN115461470A (zh) | 2020-02-28 | 2022-12-09 | 10X基因组学有限公司 | 用于从组织中分离核和细胞的方法 |
| WO2021207610A1 (en) | 2020-04-10 | 2021-10-14 | 10X Genomics, Inc. | Cold protease treatment method for preparing biological samples |
| EP4136227A1 (en) | 2020-04-16 | 2023-02-22 | 10X Genomics, Inc. | Compositions and methods for use with fixed samples |
| WO2021222302A1 (en) | 2020-04-27 | 2021-11-04 | 10X Genomics, Inc. | Methods and systems for increasing cell recovery efficiency |
| WO2021222301A1 (en) | 2020-04-27 | 2021-11-04 | 10X Genomics, Inc. | Methods and systems for analysis and identification of barcode multiplets |
| EP4244379A1 (en) | 2020-11-13 | 2023-09-20 | 10X Genomics, Inc. | Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays |
| WO2022182785A1 (en) | 2021-02-23 | 2022-09-01 | 10X Genomics, Inc. | Drug screening methods |
| CN120158497A (zh) | 2021-02-23 | 2025-06-17 | 10X基因组学有限公司 | 基于探针的核酸和蛋白质分析 |
-
2015
- 2015-06-26 EP EP25174828.1A patent/EP4574974A3/en active Pending
- 2015-06-26 WO PCT/US2015/038178 patent/WO2015200893A2/en not_active Ceased
- 2015-06-26 CN CN201580034263.7A patent/CN106795553B/zh active Active
- 2015-06-26 CN CN202110541753.3A patent/CN113249435B/zh active Active
- 2015-06-26 EP EP15811911.5A patent/EP3161160B1/en active Active
- 2015-06-26 EP EP21166784.5A patent/EP3889325A1/en active Pending
- 2015-06-26 KR KR1020167036348A patent/KR102531677B1/ko active Active
- 2015-06-26 EP EP21199180.7A patent/EP4053292A1/en active Pending
- 2015-06-26 CA CA2953374A patent/CA2953374A1/en active Pending
- 2015-06-26 CN CN202411119807.7A patent/CN118910216A/zh active Pending
- 2015-06-26 JP JP2016575111A patent/JP6838969B2/ja active Active
- 2015-06-26 AU AU2015279548A patent/AU2015279548B2/en active Active
- 2015-06-26 KR KR1020237015588A patent/KR102755843B1/ko active Active
- 2015-06-26 MX MX2016016902A patent/MX2016016902A/es unknown
- 2015-06-26 US US14/752,641 patent/US20150376609A1/en not_active Abandoned
-
2016
- 2016-12-18 IL IL249617A patent/IL249617A0/en unknown
-
2021
- 2021-02-12 JP JP2021020743A patent/JP7329552B2/ja active Active
-
2022
- 2022-07-08 US US17/860,880 patent/US11629344B2/en active Active
- 2022-11-14 US US17/986,764 patent/US11713457B2/en active Active
-
2023
- 2023-05-09 JP JP2023077527A patent/JP2023099197A/ja active Pending
- 2023-06-07 US US18/207,023 patent/US20230348897A1/en not_active Abandoned
- 2023-09-13 US US18/367,638 patent/US20240002837A1/en not_active Abandoned
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP7329552B2 (ja) | 個々の細胞または細胞集団由来の核酸の分析方法 | |
| US11359239B2 (en) | Methods and systems for processing polynucleotides | |
| US10337061B2 (en) | Methods and systems for processing polynucleotides | |
| US10273541B2 (en) | Methods and systems for processing polynucleotides | |
| US12312640B2 (en) | Analysis of nucleic acid sequences |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20180501 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20180625 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20180625 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20190717 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20191017 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20200121 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20200409 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20200720 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20201217 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20210112 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20210212 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6838969 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| RD02 | Notification of acceptance of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: R3D02 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |