CN1129954A - 新颖的脂肪酶、产生该脂肪酶的微生物、该脂肪酶的制造方法及用途 - Google Patents
新颖的脂肪酶、产生该脂肪酶的微生物、该脂肪酶的制造方法及用途 Download PDFInfo
- Publication number
- CN1129954A CN1129954A CN94193220A CN94193220A CN1129954A CN 1129954 A CN1129954 A CN 1129954A CN 94193220 A CN94193220 A CN 94193220A CN 94193220 A CN94193220 A CN 94193220A CN 1129954 A CN1129954 A CN 1129954A
- Authority
- CN
- China
- Prior art keywords
- lipase
- bacterial strain
- acid
- detergent
- pseudomonas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/874—Pseudomonas
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/82—Proteins from microorganisms
- Y10S530/825—Bacteria
Abstract
一种属于假单胞菌属(Pseudomonas)的细菌、该细菌所产生的具有如下性质的脂肪酶、该脂肪酶的制造方法及含该脂肪酶的洗涤剂组合物。
以三油酸甘油酯乳液为基质,其作用pH为3.5-12,最佳pH为10-12;其作用温度为30-80℃,最佳温度为55-65℃;以SDS-聚丙烯酰胺凝胶电泳法所测得的分子量为31000±2000;由等电点聚丙烯酰胺凝胶电泳法所测得的等电点为5.2±0.5。本发明的脂肪酶在各种市售的洗涤剂溶液中,又在与表面活性剂、蛋白酶等的洗涤剂成分共存时,具有较高的稳定性和活性,可以在洗涤条件下有效地去除油脂污垢,可增加洗涤剂的洗净力。
Description
发明领域
本发明涉及一种新颖的脂肪酶、产生该脂肪酶的微生物、该脂肪酶的制造方法及用途。更详细地说,本发明涉及一种产生细菌的、在洗涤液中(碱性域)具有活性的脂肪酶,产生该脂肪酶的微生物,该脂肪酶的制造方法,以及含有在洗涤液中可分解脂质的酶的洗涤剂组合物。
为了提高洗涤时的洗净率,以往,已知有在洗涤剂中掺和使用酶的方法。例如,在洗涤剂组合物中掺用蛋白酶,以分解去除粘附于被洗物上的蛋白质及其它污垢。另外,也有掺用纤维素酶以去除粘附于纤维素纤维被洗物上的污垢,或掺用淀粉酶等多糖分解酶以去除粘附于被洗物上的多糖及其它污垢。
近年来,更有在洗涤剂中掺用脂肪酶以分解去除粘附于被洗物上的脂类,提高洗净率的方法。其用途记载于安得利(H。Andree)等人的题为“作为洗涤剂成分的脂肪酶”(Lipase as detergent compo-nents)的报道(Journal of Applied Biochemistry,2,218—229(1980))上。
较理想的洗涤剂用的脂肪酶为在洗涤液中可充分发挥脂肪酶活性的脂肪酶。由于在通常的洗涤条件下,洗涤液的pH处于碱性区域,因此,人们便要求在碱性pH域内具有功能的脂肪酶。又,一般的脂质油污在高温/高碱的条件下比较容易去除,而在低温(60℃以下)下的洗涤则不容易充分洗净脂质油污。我国的洗涤方法从来都是以低温洗涤为主,近来,欧美各国也出现了洗涤温度低温化的倾向。因此,理想的洗涤剂用脂肪酶是在低温下也能充分发挥作用的脂肪酶。另外,理想的洗涤剂用脂肪酶在存在有表面活性剂等的洗涤剂成分,和许多种洗涤剂中含有的蛋白酶及漂白剂时,应也能充分发挥洗涤功能。而且,所述理想的洗涤剂用脂肪酶以掺和于洗涤剂中的状态保存时,对其余的洗涤剂的成分应保持稳定。人们希望开发掺有具有如上所述的特性的脂肪酶的、对脂质油污具有高洗净率的洗涤剂组合物。
已知,微生物产生的脂肪酶得自假单胞菌(Pseudomonas)属、产碱杆菌(Alcaligenes)属、消色杆菌(Achromobacter)属、毛霉菌(Mu-cor)属、假丝酵母菌(candida)属、腐植菌(Humicola)等菌属。
但得自这些菌株的脂肪酶的最合适的pH为中性至微碱性,在碱性洗涤剂溶液中不能充分发挥其性能,且在洗涤溶液中稳定性差。
再有,得自消色杆菌(Achromobacter)属、假丝酵母菌(candida)属、毛霉菌(Mucor)属、腐植菌(Humicola)属的各种脂肪酶在共存有阴离子表面活性剂时,其活性强烈受阻。
又,属于假单胞菌(Pseudomonas)属细菌的微生物产生脂肪酶,已广为人知。在产生脂肪酶的假单胞菌(Pseudomonas)属细菌中,有,Ps.(Pseudomonas)fluorescens,Ps.cepacia,Ps.fragi,Ps.alcaligenes,Ps.pseudoalcaligenes,Ps.aeruginosa。但是,从上述菌株所得的已知的脂肪酶也不能满足前述特性。
发明目的
因此,本发明的目的是,提供一种脂肪酶、产生该脂肪酶的微生物、该脂肪酶的制造方法,所述脂肪酶在洗涤液中可充分发挥功能,其活性几乎不受洗涤剂中含有的成分所阻碍,且对如表面活性剂和蛋白酶等其它的洗涤剂成分呈现高的稳定性。
又,本发明的其它目的在于提供一种含有上述脂肪酶为洗涤辅助剂的洗涤剂组合物,及除了上述脂肪酶之外,再含有蛋白酶等其它的酶而组成的含酶洗涤剂组合物。
发明的揭示
本发明者们为得到具有上述性质的脂肪酶,对许多微生物进行了分离、培养并检索,其结果,发现由从东京都地下土壤中分离出来的菌株的假单胞菌属(Pseudomonas Sp.)SD705菌株所代表的假单胞菌属(Pseudomonas)的菌株可产生出可有效地掺用于洗涤剂的新颖的脂肪酶,从而完成了本发明。
即,本发明涉及的是这样一种新的脂肪酶,该脂肪酶系由属于假单胞菌(Pseudomonas)属的微生物或其变异菌株产生,在pH3.5—12的整个反应pH范围内均有作用,其最适温度为55—65℃附近。
又,本发明涉及一种上述脂肪酶的制造方法,产生该脂肪酶的新颖微生物及含有该脂肪酶的洗涤剂组合物。
附图的简单说明
图1所示为以本发明的SD705菌株产生的脂肪酶的反应pH和相对活性之关系的图表。
图2所示为以本发明的SD705菌株产生的脂肪酶的反应温度和相对活性之关系的图表。
图3所示为将本发明的SD705菌株产生的脂肪酶在各种温度下,在pH7作一小时的处理后的残余活性的图表。
图4所示为将本发明的SD705菌株产生的脂肪酶在各种pH下,在37℃作一小时的处理后的残余活性的图表。
发明的详细说明(产生菌)
用于制造本发明的脂肪酶的微生物,可以产生出具有下述性质的脂肪酶,且,只要是具有下述分类学意义上的性质的假单胞菌(Pseudomonas)属细菌即可,并无特别限定。这种细菌可从保存的菌株中或从分离自自然界的微生物中选用。另外,这些细菌也可以是自然的或人工变异菌株,只要是可产生具有如下所述的性质的脂肪酶的细菌,皆在此范围。该些细菌菌株可按通常的方法,从土壤等其它的分离源分离而得。将被测微生物在通常的细菌用培养基中进行培养,按通常的方法,测定在高pH、常温的条件下的培养液的脂肪酶活性,由此选用目的菌株。
作为本发明的产生新颖的脂肪酶的、属于假单胞菌(Pseu-domonas)属的菌株,可举出本发明者们从东京都的地下土壤中分离的SD705菌株。
该SD705菌株的菌学性质如表1所示。表1中,参照Bergey’sManual of Systematic Bacteriology,1984,一并记载了与本菌具有比较类似的菌学性质的Ps.alcaligenes、Ps.pseudoalcaligenes的菌学性质。
表1
SD705菌株 Ps.alcaligenes Ps.Pseudoal-
caligenes(1)形态 杆菌 杆菌 杆菌(2)革兰氏染色性 阴性 阴性 阴性(3)芽胞 无 无 无(4)运动性 有 有 有(5)鞭毛 极单毛 极单毛 极单毛(6)氧化酶 阳性 阳性 阳性(7)过氧化氢酶 阳性 阳性 阳性(8)萤光色素的产生 无 无 无(9)PHB的蓄积 阴性 阴性 d(10)精氨酸二氢化酶 阴性 阳性 d(11)41℃下的生长 可 可 可(12)脱氮反应 阴性 阳性 d(13)明胶液化 阴性 d d(14)淀粉分解 阴性 阴性 阴性(15)葡萄糖的脂化性 阴性 阴性 阴性(16)天冬氨酸盐的脂化性 阴性 阴性 阴性(17)L氨酸盐的脂化性 阳性 阳性 阳性(18)D-葡萄酸盐的脂化性 阴性 阴性 d
表1(续)
SD705菌株 Ps.alcaligenes Ps.Pseudoal-
caligenes(19)L-组氨酸的脂化性 阴性 d d(20)乙醇胺的脂化性 阴性 阴性 阳性(21)正丁醇的脂化性 阳性 d 阳性(22)异丁醇的脂化性 阴性 d 阴性(23)甘油丙三醇的脂化性 阴性 阴性 d(24)山梨糖醇的脂化性 阴性 阴性 d(25)衣康酸的脂化性 阴性 阴性 d(26)中康酸的脂化性 阴性 阴性 阳性(27)β-羟基丁酸盐的脂化性 阳性 阴性 阳性(28)甜菜碱的脂化性 阴性 阴性 阳性(29)果糖的脂化性 阴性 阴性 阳性(30)丙三醇酸盐的脂化性 阴性 阴性 阳性(31)GC含量(%) 60 64~68 62~64
d:属于该菌种菌株的11~89%为阳性。
如表1所示,SD705菌株在乙醇胺、中康酸、甜菜碱、果糖、甘油酸盐的脂化性上与假单胞菌(Pseudomonas)属、pseudoalcaligenes不同,在精氨酸脱羧酶的有无、β—羟基丁酸盐的脂化性上与Ps.alcaligenes不同,且,其GC含量也较该二菌株为低。
又,根据日本细菌学杂志,45(5),1990所载,对Ps.alcaligenes的基准株ATCC909和Ps.pseudoalcaligenes的基准株ATCC17440进行定量DNA的杂种,这对于该二基准株都呈现了不到30%的相同性。由这些结果可以决定本菌株是属于假单胞菌属的Ps.alcalige-nesde的近缘新菌种。
本菌株保存于通商产业省工业技术学院生命工程工业技术研究所(National Institute of Bioscience and Human—Technology,A-gency of Industrial Science and Technology),其保存编号为FERMP—13781。以后,又根据国际上认可的有关微生物保存的专利手续上的布达佩斯条约(BUDAPEST TREATY ON THE INTERNA-TIONAL RECOGNITION OF THE DEPOSIT OF MICROOR-GANISMS FOR THE PURPOSES OF PATENTPROCEDURE),转为国际保存,其保存编号为FEREM BP—4772。
又,以上述菌株为原菌株,通过自然变异或诱导变异所得到的变异菌株也可以用作本发明的脂肪酶的生产菌。
作为上述变异菌株的配制方法,有例如,作为通常的方法,不对原菌株进行紫外线辐射处理或用N—甲基—N’—硝基—N—亚硝基胍(NTG)等药剂的人工突变处理;或者是,对原菌株进行了如上的处理,将其扩展于含有橄榄油等的琼脂培养基中,从成长的菌株中选出其周围的净区范围较大的菌丛,以生产脂肪酶的培养基进行培养,选择出生产性最优异的菌株。
本发明的脂肪酶产生菌最好是SD705菌株和在DNA杂交中显示50%以上的同系现象的菌株,更好地,是显示70%以上的同系现象的菌株。
(制造方法)
本发明的脂肪酶通过培养属于假单胞菌(Pseudomonas)属的脂肪酶产生菌,主要地产生于菌体外(培养液中)。
作为培养基的营养源,可广泛使用通常用于培养的营养源。只要是可作为碳源同化的碳化合物或者是含有该碳化合物的即可,例如,可使用油脂、Tween系的表面活性剂等。作为氮源,只要是可作为氮源同化的氮化合物或者是含有该氮化合物的即可,例如,可用铵盐、硝酸盐、大豆粉、肉类提取物、玉米浸渍液、フア—マメデイア等。又,作为无机盐类,可适当添加磷酸氢氨、磷酸氢钾等的磷酸盐、镁盐、钙盐等的盐类。
培养条件依培养基的组份而多少不同,但只要是适合于产生目的的本发明中的脂肪酶的条件即可,通常,可选择以下的条件。即,培养温度为10—40℃,更好地,为20—37℃的范围;培养时间约为8—100小时,只要在本发明的脂肪酶的生产达到最高时结束培养即可。培养基的pH可在5—12的生产范围,特别是,7—10适于本发明的脂肪酶的生产。经如上所述的培养,主要在菌体外(培养液中)产生目的物的脂肪酶。
(分离精制法)
从如上所得的培养液中采集脂肪酶,可按照通常用于采集脂肪酶的方法,由分离、精制而进行。
即,可由下述方法获得本发明的脂肪酶:由过滤法或离心分离法从培养液中分离菌体及培养基固形物,得到上清液或滤液,对该些分离液进行或不进行浓缩,添加可溶性盐类,使酶沉淀的盐析法;添加亲水性的有机溶剂,使酶或夹杂物沉淀的有机溶剂沉淀法;使用离子交换树脂等的吸附脱离法;凝胶过滤法;添加稳定辅助剂或不添加稳定辅助剂的喷雾干燥法;单独或多个组合使用冷冻干燥法等的分离或精制方法。
(酶活性的测量方法)
在本发明中,使用了以三油酸甘油酯—聚乙烯醇(PVA)乳液为基质的测量方法进行对脂肪酶活性的测量。
将含酶液0.1ml、含1mM的氯化钙、由100mM的ε-氨基己酸、100mM双三(双(2—羟乙基)亚氨基三(羟甲基)甲烷)及100mM的TAPS(N—三(羟甲基)甲基—3—氨基丙磺酸)组成的混合液用氢氧化钠调节pH(pH10.0)的缓冲液0.4ml以及三油酸甘油酯乳液0.5ml组成的混合液在带毛玻璃塞的试管中,在37℃下加热10分钟,使其反应,用1N的盐酸0.2ml作为反应停止液使反应停止。这里,三油酸甘油酯乳液用的是在聚乙烯醇(PVA)2%的水溶液(ポバ—ルPVA117(クラレ公司商品名):ポバ—ルPVA205(クラレ公司商品名)=9∶1(w/w))10ml中加入2.5g的三油酸甘油酯并经过均化的乳液。反应停止后,加入正己烷2ml、异丙醇2ml、蒸馏水1ml,激烈搅拌,静置后,将己烷层取样,以TLC—FID方法(Minagawa等,Lipids,18,732(1983))测定油酸量。以一分钟内生成1毫摩尔的油酸的酶量作为活性单位的1单位(1U)。
又,在下面的实施例中,按特公昭60—55118号上所述的活性测定方法,测量在下面的实施例中掺用的蛋白酶的活性,其活性单位表示为纳卡他尔(nkatal=10-9katal,缩写为nkat)
(酶的性质)
本发明的脂肪酶具有下述性质。即,关于本发明的假单胞菌(Pseudomonas)属的菌株SD705产生的脂肪酶,用下述性质加以记载。
(1)作用作用于甘油三酸酯,水解该酯。
(2)基质特异性可广范围地水解各种甘油三酸酯及酯类。
作为甘油三酸酯基质,使用的是各种甘油三酸酯—阿拉伯胶乳液。使用的乳液是在甘油三酸酯10g中加入阿拉伯胶10g和蒸馏水100g均化而成的乳液。
将含上述乳液5ml、100mM的氯化钠及25mM的氯化钙的10mM的三羟甲基氨基甲烷型缓冲液(pH10.0)5ml、蒸馏水4.5ml、酶液0.5ml的混合物在30℃、pH10的条件下反应,用0.05N氢氧化钠水溶液,pH起动测定法求得此时的脂肪酸生成速度。该脂肪酸的生成速度作为各基质的分解力。
如将三油酸甘油酯的分解力表示为100,则三丁酸甘油酯的相对活性显示为125,橄榄油的为55,大豆油的为70,棉籽油的为66。
另外,对于酯的分解力,曾以脂肪酸对硝基苯酯为基质,由在pH8.0、30℃下的水解反应生成的对硝基苯酚的比色(OD405)而求得。
如以pNPP(棕榈酸对硝基苯酯)的分解力为100,则pNPL(月桂酸对硝基苯酯)的相对活性显示为134,pNPV(戊酸对硝基苯酯)为34。
(3)作用pH和最佳pH
以三油酸甘油酯乳液为基质,按前述的活性测试方法进行测定。测定的pH分别为3.5~12.0范围内的不同的pH值。但是,缓冲液使用了含有1.0mM的氯化钙,由将100mM的ε-氨基己酸、100mM的双三(双(2-羟乙基)亚氨基三(羟甲基)甲烷)以及100mM的TAPS(N-三(羟甲基)甲基-3-氨基丙磺酸)组成的混合缓冲液用盐酸或氢氧化钠调节pH的缓冲液。反应pH和相对活性的关系示于图1,在pH为3.5~12的范围内测定时,作用pH为3.5~12,最佳pH为10~11。
(4)作用温度及最佳温度
以三油酸甘油酯乳液为基质,其它与前述的活性测定方法相同,在30—80℃的范围内的不同的反应温度下进行测试。反应温度和相对活性的关系示于图2,在30—80℃的温度范围内测定时,其作用温度为30—80℃,其最佳温度为55—65℃。在40及70℃时,表示了约为最佳温度时的活性的50%的相对活性。
(5)温度稳定性
按上述活性测定法,测定在20—70℃的温度范围内的不同温度下,在pH7作一小时的保温处理后的剩余活性。此时的处理温度和残余活性的关系如图3所示,60℃的处理可具有80%以上的活性。处理时的缓冲液使用了将由50mM的ε—氨基己酸、50mM的双三(双(2—羟乙基)亚氨基三(羟甲基)甲烷)以及50mM的TAPS(N—三(羟甲基)甲基—3—氨基丙磺酸)组成的混合缓冲液用盐酸调节至7的缓冲液。
(6)pH稳定性
按上述活性测定法,测定在pH4—12的范围内的不同pH下,在37℃的温度作一小时的保温处理后的剩余活性。此时的处理pH和残余活性的关系如图4所示,pH在4—10时具有50%以上的活性。处理时的缓冲液使用了将含有0.5mM的氯化钙、由50mM的ε—氨基己酸、50mM的双三(双(2—羟乙基)亚氨基三(羟甲基)甲烷)以及50mM的TAPS(N—三(羟甲基)甲基—3—氨基丙磺酸)组成的混合缓冲液用盐酸或氢氧化钠调节过pH的缓冲液。
(7)分子量
以SDS—聚丙烯酰胺凝胶电泳法(分子量标准:细胞色素C(单体、二聚物、三聚物、四聚物、五聚物))所得到的分子量为31000±2000。
(8)等电点
由等电点聚丙烯酰胺凝胶电泳法所测得的等电点为5.2±0.5。
(9)洗涤剂成分的影响
以三油酸甘油酯乳液为基质,将下述的标准使用浓度的各种市售洗涤剂(4种)和代表性的洗涤剂用的碱性蛋白酶的API—21(特公昭60—55118号)以0.3nkat/ml的浓度添加其中,调节反应pH为10,反应时间为30分钟,其余如同前述的活性测定法,进行测量。以市售的洗涤剂及不添加API—21时的活性作为100时的相对活性示于表2,在含蛋白酶的各种洗涤剂中,具有高的活性。
具有上述的性质的脂肪酶在低温下(60℃以下)也具有足够的功能,在洗涤剂溶液中也可稳定地表现其活性,因此是很适合用于洗涤剂掺用的脂肪酶。
(洗涤剂组合物)
根据本发明,可以提供具有上述性质的脂肪酶的洗涤剂组合物。掺用于本发明的洗涤剂组合物的脂肪酶的用量没有特别限制,通常是,对每g洗涤剂组合物掺用100—10000单位,更好地,对每g洗涤剂组合物掺用500—4000单位。其用量过少,则洗净效果不能充分提高;而在相反的情况下,如其用量过多,则该洗净效果并不随酶用量的增加而增加,其经济性并不理想。
按照本发明,上述脂肪酶可以在不改变洗涤剂的组成的情况下,掺用于已有公知的任一种洗涤剂组合物,对于本发明的洗涤剂组合物的成分并无特别的限制。这类洗涤剂组合物的代表性例子有:由分别占洗涤剂组合物重量的10—50%(重量)的表面活性剂,0—50%(重量)的助洗剂,1—50%(重量)的碱性剂或无机电解质,0.1—50%(重量)的选自再污染防止剂、酶、漂白剂、荧光染料、防结块剂以及抗氧化剂中的至少一种的掺用成分组成的洗涤剂组合物。
作为表面活性剂,可以使用肥皂;例如;具有直链或支链烷基或链烯基的硫酸盐、氨基硫酸盐;具有直链或支链烷基或链烯基、加合了如环氧乙烷、环氧丙烷及环氧丁烷中的单独或混合成分的烷基醚或链烯基醚那样的脂肪族硫酸化合物;如烷基磺酸盐、氨基磺酸盐、二烷基磺基琥珀酸酯、α—烯烃、亚乙烯基型烯烃及内烯烃的各种磺酸盐的脂肪族磺酸盐;具有直链或支链烷基或链烯基、加合了如环氧乙烷、环氧丙烷及环氧丁烷中的一个或多个成分的烷基或链烯基醚的羧酸盐或酰胺;α—磺基脂肪酸盐或酯;氨基酸型表面活性剂;烷基或链烯基酸性磷酸酯、烷基或链烯基磷酸酯盐的磷酸酯系表面活性剂;磺酸型两性表面活性剂;甜菜碱型两性表面活性剂;具有直链或支链烷基或链烯基、加合了如环氧乙烷、环氧丙烷及环氧丁烷中的一个或多个成分的烷基或链烯基醚或醇;具有直链或支链烷基或链烯基、加合了如环氧乙烷、环氧丙烷及环氧丁烷中的一个或多个成分的聚氧乙烯烷基苯基醚;高级脂肪酸烷醇酰胺或其环氧化物加合物;蔗糖脂肪酸酯;脂肪酸甘油单酯;烷基或链烯基胺的氧化物;四烷基铵盐型阳离子表面活性剂等,只要是通常可掺用于洗涤剂组合物的表面活性剂即可。在阴离子型的表面活性剂的场合,作为其抗衡离子最好是钠离子或钙离子。上述表面活性剂可单独或组合二种以上使用。
作为助洗剂及碱性剂或无机电解质可以使用:正磷酸盐、焦磷酸盐、三聚磷酸盐、偏磷酸盐、六偏磷酸盐、肌醇六磷酸盐等的磷酸盐;如乙烷—1,1—二膦酸、乙烷—1,1,2—三膦酸、乙烷—1—羟基—1,1—二膦酸及其衍生物;如乙烷羟基—1,1,2—三膦酸、乙烷—1,2—二羧酸—1,2—二膦酸、甲羟基膦酸等膦酸的盐;2—膦酰基丁烷—1,2—二羧酸、1—膦酰基丁烷—2,3,4—三羧酸、α—甲基膦酰基琥珀酸等膦酰基羧酸的盐;如天冬氨酸、谷氨酸等的氨基酸;如氮三乙酸盐、乙二氨四乙酸盐、二乙三氨五乙酸盐等的氨基多乙酸;如聚丙烯酸、聚衣康酸、聚马来酸、马来酸酐共聚物、羧甲基纤维素盐等的高分子电解质;如聚乙二醇、聚乙烯醇等的非离解性高分子;如二醇酸、羟基丁二酸、羧基甲氧基丁二酸、柠檬酸、乳酸、酒石酸、蔗糖、乳糖等的羧甲基化合物;如季戊四醇的羟甲基化合物;葡糖酸的羧甲基化合物;如苯多甲酸、草酸、苹果酸、羟基二琥珀酸、葡糖酸等的有机酸盐;如沸石等的铝硅酸盐;碳酸盐、倍半碳酸盐、硫酸盐、硅酸盐等作为碱金属盐的无机盐。又,也可使用如淀粉、脲等的有机物质及氯化钠、膨润土等的无机化合物。再有,作为有机碱剂,可以使用三乙醇胺、二乙醇胺、单乙醇胺、三异丙醇胺等。
本发明的洗涤剂组合物,如前所述,含有表面活性剂、脂肪酶、碱性剂及无机电解质作为必要的组成成分,但是,按需要,也可含有两性表面活性剂;如过碳酸氢钠、过硼酸氢钠等的漂白剂;色素;助洗剂;如聚乙二醇、聚乙烯醇、聚乙烯基吡咯烷酮、羧甲基纤维素等的再污染防止剂;防结块剂、抗氧化剂;蛋白酶等其它的酶。
可用任何方法将脂肪酶或蛋白酶之类的其它的酶掺用于本发明的洗涤剂组合物中。但是,如以微粉末状掺用时,则在操作洗涤剂时散发出粉尘,对洗涤剂的使用者及洗涤剂生产的操作者来说,有安全和卫生上的问题,不甚理想。最好是,以溶液状态,或预先赋与一定的形状,以控制粉尘的散发。所述赋形方法可采用通常使用的圆形造粒、挤出造粒、流动造粒、离心流动造粒及其它的方法。但是,用于本发明中的洗涤剂组合物的脂肪酶或蛋白酶之类的其它酶的形状并不限于由上述方法所赋于的形状。
下面,举实施例说明本发明,但是,本发明并不限于下述的实施例。另外,在下述的说明中,如无特别说明,则%皆指重量%。
实施例1
脂肪酶产生菌(SD705菌株)的培养
将大豆粉2%、磷酸氢二铵0.1%、橄榄油1%、磷酸氢二钾0.5%、硫酸镁七水合物0.1%及碳酸钠0.3%浓度的液体培养基2ml装入18mm口径的试验管中,在121℃作高压蒸汽灭菌20分钟后,接种假单胞菌属(Pseudomonas)SD705菌株1白金环,在35℃,于130rpm下培养24小时。培养后,离心分离去除菌体,得到脂肪酶液。所得的脂肪酶液的脂肪酶活性为5U/ml。
实施例2
脂肪酶产生菌(SD705菌株)的培养及脂肪酶的取得
将大豆粉2%、磷酸氢二铵0.1%、磷酸氢二钾0.5%、硫酸镁七水合物0.1%、碳酸钠0.3%及Tween85的1.0%浓度的液体培养基2立升装入5立升的培养槽中,在121℃作高压蒸汽灭菌20分钟后,接种假单胞菌属(Pseudomonas)SD705菌株,在35℃,于1000rpm下通气搅拌培养24小时。培养后,离心分离去除菌体,得到脂肪酶液。所得的脂肪酶液的脂肪酶活性为20U/ml。
从如此所得的脂肪酶液中,由硫酸铵沉淀法得到20—40%饱和部分的沉淀。再以通常的方法脱盐后,冷冻干燥得脂肪酶的原粉。实施例3
脂肪酶的精制
将实施例2中所得的脂肪酶原粉溶解于10%的硫酸铵溶液中,以Butyl—Toyopearl650M(商品名,东ソ株式会社)作疏水色谱,得到活性成分。将该活性成分用含有0.3mM的氯化钙的10mM的三羟甲基氨基甲烷盐酸缓冲液(pH8)作透析后,使其吸附于由该缓冲液平衡过的离子交换色谱树脂(DEAE—Cellulofine A—800,商品名,生化学株式会社)上,得到以氯化钠浓度梯度溶出的活性成分。脱盐后,冷冻干燥,得到精制的酶。
上述冷冻干燥制品经SDS聚丙烯酰胺凝胶电泳法确认为单一制品。
实施例4
本发明的脂肪酶和其它的脂肪酶在洗涤剂溶液中的活性比较
使用实施例2中所得的脂肪酶原粉,作洗涤剂溶液中的活性比较,比较美国专利第5069810号上所载的Pseudomonas alcaligenesSD2菌株(ATCC53877)所产生的脂肪酶SD2,和欧洲专利第218272号上所载的Pseudomonas Pseudoalcaligenes CBS467.85菌株、CBS468.85菌株、CBS471.85菌株、CBS473.85菌株所产生的脂肪酶在洗涤剂溶液中的活性测定结果。Pseudomonas alcaligenesSD2菌株的酶为在硫酸铵0.5%、磷酸氢二钾0.05%、硫酸镁七水合物0.025%、胰化蛋白胨2.0%、聚氧乙烯(20)十六烷基醚1.0mM的培养基中、30℃下培养16小时而得,而上述四种PseudomonasPseudoalcaligenes菌株的酶为分别将其菌株在脱脂牛奶10%、pH7的培养基中,在20℃下培养48小时,使用其经离心分离后所得的培养液上清液。
以三油酸甘油酯乳液为基质,将下述的标准使用浓度的各种市售洗涤剂(4种)和代表性的洗涤剂用的碱性蛋白酶API—21(特公昭60—55118号)以0.3nkat/ml的浓度添加其中,调节反应pH为10,反应时间为30分钟,其余如同前述的效力测定法,进行测量。这里使用的市售洗涤剂有,アタツク(商品名,花王株式会社制,直链烷基苯磺酸钠,烷基硫酸酯钠及含有聚氧乙烯烷基醚的阴离子系表面活性剂的洗涤剂);ゥルトラアリエ—ル(商品名,—·プロクタ·アソド·ギヤソブル·フア—イ—スト株式会社制;直链烷基苯磺酸钠,烷基硫酸酯钠、烷酰基羟基苯磺酸钠及含有聚氧乙烯烷基醚的阴离子系表面活性剂的洗涤剂);ゥルトラタイド(商品名,プロクタ—·アソド·ギヤソブル株式会社制,阴离子型表面活性剂)フレツシユスタ—ト(商品名,コルゲ—ト—パルモリブ株式会社制,非离子型表面活性剂)。作为标准的使用浓度,分别添加最终浓度为,アタツク833ppm,ウルトラアリエ—ル1000ppm,ウルトラタイド1000ppm,フレツシユスタ—ト624ppm。
设不添加市售洗涤剂及API—21时的活性为100,则上述洗涤剂的相对活性示于表2。
表2洗涤剂溶液中的相对活性(单位%)
洗涤剂酶产生菌株 无洗涤剂 アタツク ウルトラ ウルトラ フレツシユ
アリエ—ル タイド スタ—トSD705 100 78 84 57 98ATCC53877 100 31 29 33 80CBS 467.85 100 17 18 29 102CBS 468.85 100 54 50 55 62CBS 471.85 100 20 16 35 85CBS 473.85 100 50 49 40 95
从上表可以明白,本发明的脂肪酶比起其它的脂肪酶来,可在洗涤剂的溶液中发挥更高的活性。
即,在比较用的其它脂肪酶中,虽然,有时,有的洗涤剂也可显示与本发明的脂肪酶同等水准的相对活性,但是,如果洗涤剂的种类发生变化,则其相对活性就显著降低(来自CBS467.85菌株及CBS468.85菌株的酶)。而相比之下,本发明的脂肪酶的特征是,其所显示的高的相对活性不随洗涤剂的种类而变化。
实施例5洗涤剂组合物及洗涤效果评价
在市售洗涤剂的アタツク中,以2400u/g的比率掺用实施例2中所得的脂肪酶原粉,按本发明配制成含脂肪酶的洗涤剂组合物。
按如下所述地进行洗涤效果的评价。即,使脱脂棉布(15cm×15cm)浸透溶于苯中的三油酸甘油酯70mg,在室温下干燥过夜,用作污染布。洗净装置使用Terg—O—Tometer。在加有氯化钙的最终浓度为50ppm的蒸馏水1立升中,分别以标准使用浓度溶入前述掺和了脂肪酶的洗涤剂组合物及市售洗涤剂アタツク。在前者的场合,液中的脂肪酶浓度为2单位/ml。再有,添加蛋白酶API—21(特公昭60—55448)至0.3nkat/ml。在每1立升的洗涤液中加入上述六块三油酸甘油酯污染布,用30℃的洗涤温度、120rpm的转速洗涤30分钟。洗净后用前述含钙的蒸馏水1立升洗,共进行二次,各洗3分钟,然后,室温下干燥。未洗净及洗净后的污垢布上的三油酸甘油酯的量由用正己烷提取后的前述TLC—FID法测定,由下述的计算式求得洗净效率。其结果示于表3。
表3洗涤评价
添加有本发明的脂肪酶 不添加脂肪酶洗净效率(%) 78.4 68.5
如表3所示,在添加有本发明的脂肪酶时,其对脂质污垢的洗净效率比起不添加脂肪酶的要高。
实施例6洗涤剂组合物及洗涤效果评价
分别配制成下述三种脂肪酶/蛋白酶的洗涤剂组合物:在市售的洗涤剂才—ル(レバ—ブラザ—ズ公司制,商品名)中,以2400u/g的比率掺用实施例2中所得的脂肪酶原粉而成的掺以脂肪酶的洗涤剂组合物;在才—ル中以270nkat/g的比率掺用添加蛋白酶API—21(特公昭60—55448)配制而得的掺以蛋白酶的洗涤剂组合物;以及在才—ル中以2400u/g的比率掺用实施例2中所得的脂肪酶原粉和以270nkat/g的比率掺用添加蛋白酶API—21配制而得的掺有脂肪酶/蛋白酶的洗涤剂组合物。
按如下所述地进行洗涤效果的评价。将市售的污染布EM-PA112用作污染布。洗净装置使用Terg—O—Tometer。在添加氯化钙至最终浓度为50ppm的蒸馏水1立升中,分别按标准使用浓度(最终浓度1100ppm)溶入前述的洗涤剂组合物及市售洗涤剂才—ル。在添加了脂肪酶的场合,液中的脂肪酶浓度为2.6单位/ml。在添加了蛋白酶的洗涤剂组合物的场合,液中的蛋白酶浓度为0.3nkat/ml。在每1立升的洗涤液中加入上述六块三油酸甘油酯污染布,用30℃的洗涤温度、120rpm的转速洗涤30分钟。洗净后用前述含钙的蒸馏水1立升洗,共进行二次,各洗3分钟,然后,室温下干燥。测得洗净后的污染布的反射率(460nm)。酶对洗净的添加效果以经掺酶的洗涤剂组合物的洗净后的反射率和经不掺酶的洗涤剂组合物的洗净后的反射率之差表示。其结果示于表4。
表4洗涤评价洗涤剂组合物 添加酶的效果掺用脂肪酶 8.2掺用蛋白酶 9.8掺用脂肪酶/蛋白酶 15.6
如表4所示,在添加有本发明的脂肪酶时,不论是否添加有蛋白酶,其洗净效果皆比不添加脂肪酶的要高。
发明效果
本发明的脂肪酶在各种市售的洗涤剂溶液中,又在与表面活性剂、蛋白酶等的洗涤剂成分共存时,具有较高的稳定性和活性,可以在洗涤条件下有效地去除油脂污垢,可期望配合洗涤剂增加洗净力。
又,本发明的假单胞菌属(Pseudomonassp.)SD705菌株及在菌学上来说与其同等的菌株及其变异菌株,可有效地用于产生本发明的脂肪酶。
再有,本发明的优点在于,使用所述菌株的制造具有上述性质的脂肪酶的方法可有效地产生所述的脂肪酶。
又,通过本发明,将脂肪酶掺用于洗涤剂中,可以提供一种具有优异的洗净性能的洗涤剂组合物。
又,通过本发明,将脂肪酶及其它的酶掺用于洗涤剂中,可以提供一种具有优异的洗净性能的洗涤剂组合物。
Claims (7)
1.一种脂肪酶,其特征在于,所述脂肪酶具有下述性质:
(1)作用pH及最佳pH
以三油酸甘油酯乳液为基质,在pH范围为3.5—12内所测定的其作用pH为3.5—12,最佳pH为10—12;
(2)作用温度及最佳温度
以三油酸甘油脂乳液为基质,在温度范围为30—80℃内,所测定的作用温度为30—80℃,最佳温度为55—65℃;
(3)分子量
以SDS—聚丙烯酰胺凝胶电泳法所得到的分子量为31000±2000;
(4)等电点
由等电点聚丙烯酰胺凝胶电泳法所测得的等电点为5.2±0.5。
2.如权利要求1所述的脂肪酶,其特征在于,所述脂肪酶系从属于假单胞菌属(Pseudomonas)的细菌的培养物得到。
3.如权利要求1所述的脂肪酶,其特征在于,所述脂肪酶从假单胞菌属(Pseudomonas)SD705菌株及在菌学上来说与其同等的菌株及其变异菌株的培养物得到。
4.一种细菌、及在菌学上来说与其同等的菌株及其变异菌株,其特征在于,所述细菌属于产生权利要求1所述的脂肪酶的假单胞菌属(Pseudomonas)。
5.一种假单胞菌属(Pseudomonas sp.)SD705菌株(FERM BP—4772)、在菌学上来说与其同等的菌株及其变异菌株,其特征在于,所述菌株为产生权利要求1所述的脂肪酶的假单胞菌属(Pseu-domonas)。
6.一种脂肪酶的制造方法,其特征在于,培养如权利要求4或权利要求5所述的细菌、及在菌学上来说与其同等的菌株及其变异菌株,从培养液中回收权利要求1所述的脂肪酶。
7.一种洗涤剂组合物,其特征在于,所述洗涤剂组合物含有如权利要求1至3之任一项所述的脂肪酶。
8.一种洗涤剂组合物,其特征在于,所述洗涤剂组合物含有如权利要求1至3之任一项所述的脂肪酶及其它的酶。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP214506/1993 | 1993-08-30 | ||
JP214506/93 | 1993-08-30 | ||
JP5214506A JP2859520B2 (ja) | 1993-08-30 | 1993-08-30 | リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1129954A true CN1129954A (zh) | 1996-08-28 |
CN1072718C CN1072718C (zh) | 2001-10-10 |
Family
ID=16656848
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN94193220A Expired - Fee Related CN1072718C (zh) | 1993-08-30 | 1994-08-26 | 脂肪酶及其微生物、该脂肪酶的制造方法及用途 |
Country Status (7)
Country | Link |
---|---|
US (1) | US5827718A (zh) |
EP (1) | EP0721981A4 (zh) |
JP (1) | JP2859520B2 (zh) |
CN (1) | CN1072718C (zh) |
AU (1) | AU7508794A (zh) |
FI (1) | FI960949A0 (zh) |
WO (1) | WO1995006720A1 (zh) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100419126C (zh) * | 2005-06-16 | 2008-09-17 | 广州天至环保科技有限公司 | 一种应用于金属加工业前处理除油脱脂的生物除油剂及其处理金属表面油污的方法 |
CN102226173A (zh) * | 2011-05-06 | 2011-10-26 | 华南理工大学 | 一种稳定型酶制剂及其制备方法和应用 |
CN103451013A (zh) * | 2013-09-25 | 2013-12-18 | 陕西合盛生物柴油技术开发有限公司 | 废弃油脂的预处理方法 |
CN107488615A (zh) * | 2017-09-18 | 2017-12-19 | 山东隆科特酶制剂有限公司 | 一株高产脂肪酶的假单胞菌及其发酵产酶方法 |
Families Citing this family (669)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08228778A (ja) * | 1995-02-27 | 1996-09-10 | Showa Denko Kk | 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法 |
JPH08238088A (ja) * | 1995-03-06 | 1996-09-17 | Showa Denko Kk | 高温下で高活性を有するリパーゼ |
US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
JPH09275977A (ja) * | 1996-04-18 | 1997-10-28 | Novo Nordisk As | 新規リパーゼおよび洗浄剤組成物 |
WO1998017790A1 (fr) * | 1996-10-17 | 1998-04-30 | Novo Nordisk A/S | Lipase, procede de production de cette lipase, micro-organisme produisant cette derniere et utilisation de celle-ci |
US6156552A (en) * | 1998-02-18 | 2000-12-05 | Novo Nordisk A/S | Lipase variants |
ATE231186T1 (de) | 1998-07-21 | 2003-02-15 | Danisco | Lebensmittel |
TR200101497T2 (tr) | 1998-11-27 | 2001-11-21 | Novozymes A/S | Lipolitik enzim varyantları |
ATE504651T1 (de) | 1998-12-18 | 2011-04-15 | Novozymes As | Subtilase enzyme der i-s1- und i-s2-untergruppen mit einem zusätzlichen aminosäurerest in einer aktiven schleifenregion |
AU3419200A (en) | 1999-03-31 | 2000-10-23 | Novozymes A/S | Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same |
DK2011864T3 (en) | 1999-03-31 | 2015-04-07 | Novozymes As | Polypeptides with alkaline alpha-amylase activity and nucleic acids encoding them |
WO2000071690A1 (en) | 1999-05-20 | 2000-11-30 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129 |
ATE408679T1 (de) | 1999-05-20 | 2008-10-15 | Novozymes As | Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 127 und 128 |
ATE408676T1 (de) | 1999-05-20 | 2008-10-15 | Novozymes As | Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 132 und 133 |
WO2000071688A1 (en) | 1999-05-20 | 2000-11-30 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 126 and 127 |
DE60040284D1 (de) | 1999-05-20 | 2008-10-30 | Novozymes As | Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130 |
EP1183343B2 (en) | 1999-05-20 | 2013-11-27 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 125 and 126 |
EP2336331A1 (en) | 1999-08-31 | 2011-06-22 | Novozymes A/S | Novel proteases and variants thereof |
AU7961401A (en) | 2000-08-21 | 2002-03-04 | Novozymes As | Subtilase enzymes |
WO2002031133A1 (en) | 2000-10-13 | 2002-04-18 | Novozymes A/S | Subtilase variants |
EP1975229A3 (en) | 2000-10-13 | 2009-03-18 | Novozymes A/S | Alpha-amylase variant with altered properties |
DE60234523D1 (de) | 2001-05-15 | 2010-01-07 | Novozymes As | Alpha-amylasevariante mit veränderten eigenschaften |
BR0209154A (pt) | 2001-05-18 | 2004-07-20 | Danisco | Processo de preparação de uma massa com uma enzima |
EP2295544B1 (en) | 2001-06-26 | 2016-06-22 | Novozymes A/S | Polypeptides having cellobiohydrolase I activity and polynucleotides encoding same |
DK200101090A (da) | 2001-07-12 | 2001-08-16 | Novozymes As | Subtilase variants |
ATE512979T1 (de) * | 2002-02-19 | 2011-07-15 | Procter & Gamble | Neue lipase aus pilzen |
EP1490485B1 (en) | 2002-03-27 | 2015-03-04 | Novozymes A/S | Granules with filamentous coatings |
WO2004003187A2 (en) | 2002-07-01 | 2004-01-08 | Novozymes A/S | Mpg added to fermentation |
EP2302046B1 (en) | 2002-10-01 | 2012-01-04 | Novozymes A/S | Family GH 61 polypeptides |
US20060135433A1 (en) | 2002-10-08 | 2006-06-22 | Murray Christopher J | Phenolic binding peptides |
TWI319007B (en) | 2002-11-06 | 2010-01-01 | Novozymes As | Subtilase variants |
CN1738899A (zh) | 2002-12-11 | 2006-02-22 | 诺和酶股份有限公司 | 洗涤剂组合物 |
CN101555472A (zh) | 2002-12-20 | 2009-10-14 | 诺维信公司 | 具有纤维二糖水解酶ⅱ活性的多肽及编码它的多核苷酸 |
MXPA05007654A (es) | 2003-01-17 | 2005-09-30 | Danisco | Metodo. |
US7955814B2 (en) | 2003-01-17 | 2011-06-07 | Danisco A/S | Method |
US20050196766A1 (en) | 2003-12-24 | 2005-09-08 | Soe Jorn B. | Proteins |
CN1742084B (zh) | 2003-01-27 | 2010-09-08 | 诺维信公司 | 颗粒的稳定化 |
US20060205628A1 (en) | 2003-02-18 | 2006-09-14 | Novozymes A/S | Detergent compositions |
EP2853593B1 (en) | 2003-03-07 | 2017-10-04 | DSM IP Assets B.V. | Hydrolases, nucleic acids encoding them and mehods for making and using them |
DK1622921T3 (da) | 2003-05-02 | 2010-09-13 | Novozymes Inc | Varianter af beta-glucosidaser |
WO2004111221A1 (en) | 2003-06-19 | 2004-12-23 | Novozymes A/S | Proteases |
EP1675941B1 (en) | 2003-06-25 | 2013-05-22 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
US20050048619A1 (en) | 2003-08-25 | 2005-03-03 | Novozymes Biotech, Inc. | Variants of glycoside hydrolases |
ES2358092T3 (es) | 2003-10-10 | 2011-05-05 | Novozymes A/S | Variantes de proteasa. |
ATE516347T1 (de) | 2003-10-23 | 2011-07-15 | Novozymes As | Protease mit verbesserter stabilität in detergentien |
CN1902310B (zh) | 2003-10-28 | 2011-09-21 | 诺维信股份有限公司 | 具有β-葡糖苷酶活性的多肽和编码所述多肽的多核苷酸 |
CN1875098A (zh) | 2003-10-30 | 2006-12-06 | 诺和酶股份有限公司 | 新家族的碳水化合物结合组件 |
US7906307B2 (en) | 2003-12-24 | 2011-03-15 | Danisco A/S | Variant lipid acyltransferases and methods of making |
US7718408B2 (en) | 2003-12-24 | 2010-05-18 | Danisco A/S | Method |
GB0716126D0 (en) | 2007-08-17 | 2007-09-26 | Danisco | Process |
WO2005066339A2 (en) | 2004-01-06 | 2005-07-21 | Novozymes A/S | Polypeptides of alicyclobacillus sp. |
DK1713825T3 (da) | 2004-01-30 | 2012-11-05 | Novozymes Inc | Polypeptider med cellulolytisk forøgende aktivitet og polynukleotider der koder for samme |
JP2007521823A (ja) | 2004-02-13 | 2007-08-09 | ノボザイムス アクティーゼルスカブ | プロテアーゼ変異型 |
GB0405637D0 (en) | 2004-03-12 | 2004-04-21 | Danisco | Protein |
US7148404B2 (en) | 2004-05-04 | 2006-12-12 | Novozymes A/S | Antimicrobial polypeptides |
MXPA06014649A (es) | 2004-06-21 | 2007-03-12 | Novozymes As | Proteasas. |
CN107151662B (zh) | 2004-07-05 | 2021-06-29 | 诺维信公司 | 具有改变特性的α-淀粉酶变异体 |
DK1776455T3 (da) | 2004-07-16 | 2015-06-22 | Dupont Nutrition Biosci Aps | Lipolytisk enzym, anvendelser deraf i fødevareindustrien |
WO2006032278A1 (en) | 2004-09-21 | 2006-03-30 | Novozymes A/S | Subtilases |
US7741095B2 (en) | 2004-09-21 | 2010-06-22 | Novozymes A/S | Subtilases |
WO2006032279A1 (en) | 2004-09-21 | 2006-03-30 | Novozymes A/S | Subtilases |
WO2006039541A2 (en) | 2004-09-30 | 2006-04-13 | Novozymes, Inc. | Polypeptides having lipase activity and polynucleotides encoding same |
CN101253263B (zh) | 2005-04-27 | 2014-07-02 | 诺维信股份有限公司 | 具有内切葡聚糖酶活性的多肽和编码该多肽的多核苷酸 |
EP2290061A3 (en) | 2005-07-08 | 2011-07-06 | Novozymes A/S | Subtilase variants |
WO2007019859A2 (en) | 2005-08-16 | 2007-02-22 | Novozymes A/S | Polypeptides of strain bacillus sp. p203 |
JP5329958B2 (ja) | 2005-08-16 | 2013-10-30 | ノボザイムス アクティーゼルスカブ | サブチラーゼ |
NZ589570A (en) | 2005-09-30 | 2012-06-29 | Novozymes Inc | Methods for enhancing the degradation or conversion of cellulosic material |
US9303256B2 (en) | 2005-09-30 | 2016-04-05 | Novozymes A/S | Immobilization of enzymes |
US8071089B2 (en) * | 2005-11-01 | 2011-12-06 | Bio-Cat, Inc. | Composition with a fungal (yeast) lipase and method for treating lipid malabsorption in cystic fibrosis as well as people suffering from pancreatic lipase insufficiency |
US20070179074A1 (en) * | 2006-01-23 | 2007-08-02 | Souter Philip F | Detergent compositions |
US20070191248A1 (en) * | 2006-01-23 | 2007-08-16 | Souter Philip F | Detergent compositions |
US20090324574A1 (en) | 2006-02-02 | 2009-12-31 | Verenium Corporation | Esterases and Related Nucleic Acids and Methods |
EP1994130A1 (en) | 2006-03-02 | 2008-11-26 | Novozymes A/S | High capacity encapsulation process |
WO2007107573A1 (en) | 2006-03-22 | 2007-09-27 | Novozymes A/S | Use of polypeptides having antimicrobial activity |
EP2383330A1 (en) | 2006-03-31 | 2011-11-02 | Novozymes A/S | A stabilized liquid enzyme composition |
MX2008012999A (es) | 2006-04-14 | 2008-10-17 | Genencor Int | Tratamiento de una etapa de textiles. |
CN101516906B (zh) | 2006-07-21 | 2013-11-06 | 诺维信股份有限公司 | 提高具有生物学活性之多肽的分泌的方法 |
WO2008021761A2 (en) | 2006-08-11 | 2008-02-21 | Novozymes Biologicals, Inc. | Bacteria cultures and compositions comprising bacteria cultures |
BRPI0718506A2 (pt) | 2006-09-22 | 2013-11-12 | Danisco Us Inc Genencor Div | Pectato liase e método de uso para biolimpeza |
WO2008040818A1 (en) | 2006-10-06 | 2008-04-10 | Novozymes A/S | Detergent compositions and the use of enzyme combinations therein |
JP2010512787A (ja) | 2006-12-21 | 2010-04-30 | ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン | バシルス(Bacillus)種195のアルファ‐アミラーゼポリペプチドの組成物と使用。 |
BRPI0720801A2 (pt) | 2007-01-25 | 2014-09-16 | Dupont Nutrition Biosci Aps | Produção de um lipídio aciltranfersase a partir de células transformadas de bacillus licheniformis. |
JP2010520767A (ja) | 2007-03-09 | 2010-06-17 | ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン | アルカリ性バシルス種(Bacillussp.)アルファ‐アミラーゼ変異体、アルファ‐アミラーゼ変異体を含む組成物及び使用方法。 |
ES2440741T3 (es) | 2007-03-23 | 2014-01-30 | Novozymes Biologicals, Inc. | Prevención y reducción de la formación de biopelícula y proliferación planctónica |
DE102007016139A1 (de) | 2007-03-30 | 2008-10-02 | Jenabios Gmbh | Verfahren zur regioselektiven Oxygenierung von N-Heterozyklen |
US20090023624A1 (en) * | 2007-07-06 | 2009-01-22 | Xiaomei Niu | Detergent compositions |
DE102007047433A1 (de) | 2007-10-04 | 2009-04-09 | Lanxess Deutschland Gmbh | Flüssigwasch- und Flüssigreinigungsmittel |
MX2010004670A (es) | 2007-11-05 | 2010-05-20 | Danisco Us Inc | Variantes de alfa-amilasa ts-23 de bacillus sp. con propiedades alteradas. |
AR069168A1 (es) | 2007-11-05 | 2010-01-06 | Danisco Us Inc Genencor Div | Variantes de alfa -amilasas con propiedades alteradas |
WO2009100102A2 (en) | 2008-02-04 | 2009-08-13 | Danisco Us Inc., Genencor Division | Ts23 alpha-amylase variants with altered properties |
US20090217463A1 (en) * | 2008-02-29 | 2009-09-03 | Philip Frank Souter | Detergent composition comprising lipase |
CN102112602A (zh) * | 2008-02-29 | 2011-06-29 | 宝洁公司 | 包含脂肪酶的洗涤剂组合物 |
EP2283136A2 (en) | 2008-04-30 | 2011-02-16 | Danisco US Inc. | New chimeric alpha-amylase variants |
DK2291526T3 (da) | 2008-06-06 | 2014-10-06 | Danisco Us Inc | Saccharificering af enzymsammensætning med bacillus subtilis alpha-amylase |
CA2726630A1 (en) * | 2008-06-06 | 2009-12-10 | Danisco Us Inc. | Production of glucose from starch using alpha-amylases from bacillus subtilis |
WO2009149130A2 (en) | 2008-06-06 | 2009-12-10 | Danisco Us Inc. | Geobacillus stearothermophilus alpha-amylase (amys) variants with improved properties |
US8323945B2 (en) * | 2008-06-06 | 2012-12-04 | Danisco Us Inc. | Variant alpha-amylases from Bacillus subtilis and methods of uses, thereof |
EP2149786A1 (en) | 2008-08-01 | 2010-02-03 | Unilever PLC | Improvements relating to detergent analysis |
US8198062B2 (en) | 2008-08-29 | 2012-06-12 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and methods for making and using them |
US8153391B2 (en) | 2008-08-29 | 2012-04-10 | Bunge Oils, Inc. | Hydrolases, nucleic acids encoding them and methods for making and using them |
US8357503B2 (en) | 2008-08-29 | 2013-01-22 | Bunge Oils, Inc. | Hydrolases, nucleic acids encoding them and methods for making and using them |
DE212009000119U1 (de) | 2008-09-12 | 2011-12-30 | Unilever N.V. | Spender und Vorbehandlungsmittel für viskose Flüssigkeiten |
MX2011003178A (es) | 2008-09-25 | 2011-04-21 | Danisco Inc | Mezclas de alfa-amilasa y metodos para usar esas mezclas. |
EP2358878B1 (en) | 2008-11-20 | 2014-10-15 | Novozymes Inc. | Polypeptides having amylolytic enhancing activity and polynucleotides encoding same |
CN102300986A (zh) | 2008-12-04 | 2011-12-28 | 诺维信股份有限公司 | 具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸 |
US20110296557A1 (en) | 2008-12-12 | 2011-12-01 | Novozymes, Inc. | Polypeptides Having Lipase Activity And Polynucleotides Encoding Same |
EP2202290A1 (en) | 2008-12-23 | 2010-06-30 | Unilever PLC | A flowable laundry composition and packaging therefor |
EP2213723A1 (en) | 2009-01-30 | 2010-08-04 | Novozymes A/S | Isomaltose for fungus fermentation |
WO2010097436A1 (en) | 2009-02-27 | 2010-09-02 | Novozymes A/S | Mutant cells having reduced expression of metallopeptidase, suitable for recombinant polypeptide production |
US20120172275A1 (en) | 2009-03-10 | 2012-07-05 | Danisco Us Inc. | Bacillus Megaterium Strain DSM90-Related Alpha-Amylases, and Methods of Use, Thereof |
US8293697B2 (en) | 2009-03-18 | 2012-10-23 | The Procter & Gamble Company | Structured fluid detergent compositions comprising dibenzylidene sorbitol acetal derivatives |
US8153574B2 (en) | 2009-03-18 | 2012-04-10 | The Procter & Gamble Company | Structured fluid detergent compositions comprising dibenzylidene polyol acetal derivatives and detersive enzymes |
RU2011143721A (ru) | 2009-04-01 | 2013-05-10 | ДАНИСКО ЮЭс ИНК. | Система очистки, содержащая альфа-амилазу и протеазу |
WO2010117511A1 (en) | 2009-04-08 | 2010-10-14 | Danisco Us Inc. | Halomonas strain wdg195-related alpha-amylases, and methods of use, thereof |
US8439980B2 (en) | 2009-05-05 | 2013-05-14 | Conopco, Inc. | Shading composition |
EP2451922A1 (en) | 2009-07-09 | 2012-05-16 | The Procter & Gamble Company | Method of laundering fabric using a compacted liquid laundry detergent composition |
WO2011005917A1 (en) | 2009-07-09 | 2011-01-13 | The Procter & Gamble Company | Method of laundering fabric using a liquid laundry detergent composition |
EP2451919A1 (en) | 2009-07-09 | 2012-05-16 | The Procter & Gamble Company | Method of laundering fabric using a liquid laundry detergent composition |
MX342487B (es) | 2009-07-09 | 2016-09-29 | The Procter & Gamble Company * | Composicion detergente solida para tratamiento de tela con bajo contenido de aditivo. ligeramente alcalina, que comprende acido ftalimido peroxicaproico. |
EP2478096B1 (en) | 2009-09-17 | 2017-05-24 | Novozymes, Inc. | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
BR112012006497A2 (pt) | 2009-09-25 | 2015-09-08 | Novozymes As | uso de uma variante de subtilisina, composição de lavagem de pratos, e, uso de uma composição. |
CN102648273B (zh) | 2009-09-25 | 2017-04-26 | 诺维信公司 | 枯草蛋白酶变体 |
CN102695719B (zh) | 2009-09-29 | 2016-03-09 | 诺维信股份有限公司 | 具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸 |
BR112012007390A2 (pt) | 2009-09-30 | 2015-09-15 | Novozymes As | polipeptídeo isolado tendo atividade intensificadora celulolítica, polinucleotídeo isolado, métodos para produzir o polipeptídeo, para produzir um mutante de uma célula precursora, para inibir a expressão de um polipeptídeo, para produzir uma proteína, para degragar ou converter um material celulósico, para produzir um produto de fermentação, e para fermentar um material celulósico, planta transgênica, parte de planta ou célula de planta transformada com um polinucleotídeo, molécula inibidora de filamento duplo, e, composição detergente |
ES2551141T3 (es) | 2009-09-30 | 2015-11-16 | Novozymes Inc. | Polipéptidos derivados de Thermoascus crustaceus con actividad de aumento celulolítico y polinucleótidos que codifican los mismos |
PL2519624T3 (pl) | 2009-10-08 | 2015-04-30 | Unilever Nv | Cieniująca kompozycja |
EP2488622B1 (en) | 2009-10-13 | 2014-12-17 | Unilever PLC | Dye polymers |
CN102666868A (zh) | 2009-10-21 | 2012-09-12 | 诺维信公司 | 油处理方法 |
WO2011049945A2 (en) | 2009-10-23 | 2011-04-28 | Danisco Us Inc. | Methods for reducing blue saccharide |
EP2491105B2 (en) | 2009-10-23 | 2018-02-28 | Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House | Dye polymers |
CN102666824A (zh) | 2009-12-21 | 2012-09-12 | 丹尼斯科美国公司 | 能够改进经脂肪酶处理的脂质基污渍的清洁的表面活性剂 |
WO2011080267A2 (en) | 2009-12-29 | 2011-07-07 | Novozymes A/S | Polypetides having detergency enhancing effect |
CN102753672B (zh) | 2010-01-07 | 2014-11-12 | 荷兰联合利华有限公司 | 天然调色剂 |
AU2011208429B2 (en) | 2010-01-22 | 2014-05-22 | Dupont Nutrition Biosciences Aps | Methods for producing amino-substituted glycolipid compounds |
ZA201205562B (en) | 2010-02-09 | 2013-09-25 | Unilever Plc | Dye polymers |
MX369096B (es) | 2010-02-10 | 2019-10-29 | Novozymes As | Variantes y composiciones que comprenden variantes con alta estabilidad en presencia de un agente quelante. |
EP2357220A1 (en) | 2010-02-10 | 2011-08-17 | The Procter & Gamble Company | Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent |
WO2011098356A1 (en) | 2010-02-12 | 2011-08-18 | Unilever Plc | Laundry treatment composition comprising bis-azo shading dyes |
US8859259B2 (en) | 2010-02-14 | 2014-10-14 | Ls9, Inc. | Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols |
US8815559B2 (en) | 2010-02-18 | 2014-08-26 | Danisco Us Inc. | Amylase from nesterenkonia and methods of use, thereof |
US8865637B2 (en) | 2010-02-25 | 2014-10-21 | Novozymes Als | Variants of a lysozyme and polynucleotides encoding same |
EP2380957A1 (en) | 2010-04-19 | 2011-10-26 | The Procter & Gamble Company | Solid laundry detergent composition having a dynamic in-wash ph profile |
EP2365059A1 (en) | 2010-03-01 | 2011-09-14 | The Procter & Gamble Company | Solid laundry detergent composition comprising C.I. fluorescent brightener 260 in alpha-crystalline form |
EP2377914B1 (en) | 2010-04-19 | 2016-11-09 | The Procter & Gamble Company | Mildly alkaline, low-built, solid fabric treatment detergent composition comprising perhydrolase |
EP2365054A1 (en) | 2010-03-01 | 2011-09-14 | The Procter & Gamble Company | Solid laundry detergent composition comprising secondary alcohol-based detersive surfactant |
EP2363456A1 (en) | 2010-03-01 | 2011-09-07 | The Procter & Gamble Company | Solid laundry detergent composition comprising brightener in micronized particulate form |
EP2365058A1 (en) | 2010-03-01 | 2011-09-14 | The Procter & Gamble Company | Solid laundry detergent composition having an excellent anti-encrustation profile |
US20110257062A1 (en) | 2010-04-19 | 2011-10-20 | Robert Richard Dykstra | Liquid laundry detergent composition comprising a source of peracid and having a ph profile that is controlled with respect to the pka of the source of peracid |
US20110257060A1 (en) | 2010-04-19 | 2011-10-20 | Robert Richard Dykstra | Laundry detergent composition comprising bleach particles that are suspended within a continuous liquid phase |
US8889612B2 (en) | 2010-04-19 | 2014-11-18 | The Procter & Gamble Company | Method of laundering fabric using a compacted liquid laundry detergent composition |
US20110257069A1 (en) | 2010-04-19 | 2011-10-20 | Stephen Joseph Hodson | Detergent composition |
EP2563893B1 (en) | 2010-04-29 | 2014-05-14 | Unilever PLC | Bis-heterocyclic azo dyes |
EP2395071A1 (en) | 2010-06-10 | 2011-12-14 | The Procter & Gamble Company | Solid detergent composition comprising lipase of bacterial origin |
EP2395070A1 (en) | 2010-06-10 | 2011-12-14 | The Procter & Gamble Company | Liquid laundry detergent composition comprising lipase of bacterial origin |
WO2011163457A1 (en) | 2010-06-23 | 2011-12-29 | The Procter & Gamble Company | Product for pre-treatment and laundering of stained fabric |
BR112013000114B1 (pt) | 2010-07-22 | 2020-12-29 | Unilever N.V. | composição de limpeza e processo para limpeza de um substrato |
ES2561553T3 (es) | 2010-07-22 | 2016-02-26 | Unilever N.V. | Combinaciones de ramnolípidos y enzimas para una limpieza mejorada |
WO2012028483A1 (en) | 2010-08-30 | 2012-03-08 | Novozymes A/S | A concentrated soak wash |
JP2013536698A (ja) | 2010-08-30 | 2013-09-26 | ノボザイムス アクティーゼルスカブ | 2回浸漬洗浄 |
EP2616483A1 (en) | 2010-09-16 | 2013-07-24 | Novozymes A/S | Lysozymes |
GB201015672D0 (en) | 2010-09-20 | 2010-10-27 | Unilever Plc | Improvements relating to fabric treatment compositions comprising targeted benefit agents |
BR112013007693B1 (pt) | 2010-09-30 | 2021-12-07 | Novozymes, Inc | Variante, polinucleotídeo isolado, célula hospedeira microbiana recombinante, métodos para produzir uma variante, para produzir uma variante tendo atividade intensificadora celulolítica, para degradar um material celulósico, para produzir um produto de fermentação, para fermentar um material celulósico, e, composição enzimática, formulação do caldo completo ou composição de cultura de células |
US9816082B2 (en) | 2010-09-30 | 2017-11-14 | Novozymes, Inc. | Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
WO2012048956A1 (en) | 2010-10-14 | 2012-04-19 | Unilever Plc | Packaged concentrated particulate detergent composition |
MX2013003934A (es) | 2010-10-14 | 2013-06-28 | Unilever Nv | Composiciones de detergente particulado que comprenden agente fluorescente. |
CA2813789C (en) | 2010-10-14 | 2020-07-21 | Unilever Plc | Laundry detergent particles |
EP2441822A1 (en) | 2010-10-14 | 2012-04-18 | Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House | Laundry detergent particles |
PT2627755E (pt) | 2010-10-14 | 2016-01-14 | Unilever Nv | Composição de detergente particulada empacotada |
CN103153812B (zh) | 2010-10-14 | 2016-04-06 | 荷兰联合利华有限公司 | 洗涤剂组合物的透明包装 |
EP2627758B1 (en) | 2010-10-14 | 2016-11-02 | Unilever PLC | Laundry detergent particles |
WO2012049032A1 (en) | 2010-10-14 | 2012-04-19 | Unilever Plc | Refill and refillable packages of concentrated particulate detergent compositions |
CN103180427B (zh) | 2010-10-14 | 2016-02-17 | 荷兰联合利华有限公司 | 洗涤剂组合物的包装和分配 |
WO2012049034A1 (en) | 2010-10-14 | 2012-04-19 | Unilever Plc | Packaging and dispensing of detergent compositions |
EP2441825A1 (en) | 2010-10-14 | 2012-04-18 | Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House | Process for preparing laundry detergent particles |
CA2813791C (en) | 2010-10-14 | 2020-07-28 | Unilever Plc | Laundry detergent particles |
EP2441820A1 (en) | 2010-10-14 | 2012-04-18 | Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House | Laundry detergent particles |
WO2012049033A1 (en) | 2010-10-14 | 2012-04-19 | Unilever Plc | Top-loading laundry vessel method |
CN103154225B (zh) | 2010-10-14 | 2015-02-04 | 荷兰联合利华有限公司 | 洗衣洗涤剂颗粒 |
US8883702B2 (en) | 2010-10-14 | 2014-11-11 | Conopco, Inc. | Packaged particulate detergent composition |
CN103153813B (zh) | 2010-10-14 | 2015-08-19 | 荷兰联合利华有限公司 | 包含洗衣组合物的包装、所述包装的分配器和利用所述分配器和所述包装进行洗涤的方法 |
PL2627754T3 (pl) | 2010-10-14 | 2017-06-30 | Unilever N.V. | Cząstki detergentowe do prania |
BR112013009132B1 (pt) | 2010-10-14 | 2021-12-14 | Unilever Ip Holdings B.V. | Partícula de detergente revestida e pluralidade de partículas de detergente revestidas |
CN103180423B (zh) | 2010-10-22 | 2015-08-12 | 荷兰联合利华有限公司 | 外部结构化的水性洗涤剂液体 |
WO2012059363A1 (en) | 2010-11-01 | 2012-05-10 | Unilever Nv | A detergent composition having shading dyes and lipase |
EP2640833B1 (en) | 2010-11-18 | 2016-08-24 | Novozymes, Inc. | Chimeric polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
WO2012098046A1 (en) | 2011-01-17 | 2012-07-26 | Unilever Plc | Dye polymer for laundry treatment |
AR084876A1 (es) | 2011-01-21 | 2013-07-10 | Novozymes As | Produccion de esteres alquilicos de acidos grasos |
ES2538997T3 (es) | 2011-01-31 | 2015-06-25 | Unilever N.V. | Composiciones detergentes líquidas alcalinas |
US9347080B2 (en) | 2011-01-31 | 2016-05-24 | Novozymes A/S | Use of browned glucose as a feed substrate |
AR087146A1 (es) | 2011-02-04 | 2014-02-26 | Novozymes As | Proceso para la preparacion de derivados de acidos carboxilicos |
EP2675891B1 (en) | 2011-02-15 | 2018-06-20 | Novozymes Biologicals, Inc. | Mitigation of odor in cleaning machines and cleaning processes |
US20140038876A1 (en) | 2011-02-16 | 2014-02-06 | Novozymes A/S | Detergent Compositions Comprising Mettaloproteases |
US20140038270A1 (en) | 2011-02-16 | 2014-02-06 | Novozymes A/S | Detergent Compositions Comprising Metalloproteases |
JP2014508830A (ja) | 2011-02-16 | 2014-04-10 | ノボザイムス アクティーゼルスカブ | 金属プロテアーゼを含む洗剤組成物 |
MX2013007997A (es) | 2011-02-23 | 2013-08-21 | Novozymes Inc | Polipeptidos que tienen actividad de incremento celulolitico y polinucleotidos que codifican para los mismos. |
VN36510A1 (en) | 2011-03-10 | 2014-01-27 | Unilever Plc No 41424 | Dye polymer |
WO2012130492A1 (en) | 2011-03-25 | 2012-10-04 | Unilever Plc | Dye polymer |
EP2691532A1 (en) | 2011-03-30 | 2014-02-05 | Novozymes A/S | Esterification process |
WO2012135659A2 (en) | 2011-03-31 | 2012-10-04 | Novozymes A/S | Methods for enhancing the degradation or conversion of cellulosic material |
EP2476743B1 (en) | 2011-04-04 | 2013-04-24 | Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House | Method of laundering fabric |
WO2012149344A1 (en) | 2011-04-29 | 2012-11-01 | Novozymes, Inc. | Methods for enhancing the degradation or conversion of cellulosic material |
EP2522715A1 (en) | 2011-05-13 | 2012-11-14 | Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House | Aqueous concentrated laundry detergent compositions |
EP2522714A1 (en) | 2011-05-13 | 2012-11-14 | Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House | Aqueous concentrated laundry detergent compositions |
BR112013021581A2 (pt) | 2011-05-26 | 2016-11-16 | Unilever Nv | composição detergente líquida para lavar roupa e método de tratamento de um têxtil |
EP4134424A1 (en) | 2011-06-01 | 2023-02-15 | Unilever IP Holdings B.V. | Liquid detergent composition containing dye polymer |
CN107475235B (zh) | 2011-06-20 | 2022-09-13 | 诺维信公司 | 颗粒组合物 |
EP2537918A1 (en) | 2011-06-20 | 2012-12-26 | The Procter & Gamble Company | Consumer products with lipase comprising coated particles |
US20120324655A1 (en) | 2011-06-23 | 2012-12-27 | Nalini Chawla | Product for pre-treatment and laundering of stained fabric |
EP2723858B1 (en) | 2011-06-24 | 2017-04-12 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
PL3543333T3 (pl) | 2011-06-30 | 2022-06-13 | Novozymes A/S | Sposób badania przesiewowego alfa-amylaz |
EP2540824A1 (en) | 2011-06-30 | 2013-01-02 | The Procter & Gamble Company | Cleaning compositions comprising amylase variants reference to a sequence listing |
KR20140056237A (ko) | 2011-06-30 | 2014-05-09 | 노보자임스 에이/에스 | 알파-아밀라제 변이체 |
BR112013033816A2 (pt) | 2011-07-01 | 2017-02-14 | Novozymes A / S | composição de detergente líquido sem boro |
IN2014CN00700A (zh) | 2011-07-01 | 2015-04-03 | Novozymes As | |
US10711262B2 (en) | 2011-07-12 | 2020-07-14 | Novozymes A/S | Storage-stable enzyme granules |
ES2550051T3 (es) | 2011-07-21 | 2015-11-04 | Unilever N.V. | Composición líquida para el lavado de ropa |
EP2551335A1 (en) | 2011-07-25 | 2013-01-30 | The Procter & Gamble Company | Enzyme stabilized liquid detergent composition |
CN103748219A (zh) | 2011-08-15 | 2014-04-23 | 诺维信公司 | 具有纤维素酶活性的多肽以及编码它的多核苷酸 |
CN103748205A (zh) | 2011-08-18 | 2014-04-23 | 荷兰联合利华有限公司 | 酶体系 |
EP2744900B1 (en) | 2011-08-19 | 2017-07-19 | Novozymes A/S | Polypeptides having protease activity |
CN103946359A (zh) | 2011-09-15 | 2014-07-23 | 荷兰联合利华有限公司 | 包含表面活性剂和酶的洗涤剂组合物 |
EP2751266B1 (en) | 2011-09-22 | 2017-03-29 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
EP3246404B1 (en) | 2011-10-28 | 2019-02-27 | Danisco US Inc. | Variant maltohexaose-forming alpha-amylase variants |
EP3597736A1 (en) | 2011-11-21 | 2020-01-22 | Novozymes A/S | Gh61 polypeptide variants and polynucleotides encoding same |
WO2013076269A1 (en) | 2011-11-25 | 2013-05-30 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
ES2624531T3 (es) | 2011-11-25 | 2017-07-14 | Novozymes A/S | Polipéptidos que tienen actividad de lisozima y polinucleótidos que codifican los mismos |
EP3272862A1 (en) | 2011-12-16 | 2018-01-24 | Novozymes, Inc. | Polypeptides having laccase activity and polynucleotides encoding same |
WO2013092051A1 (en) | 2011-12-20 | 2013-06-27 | Unilever Plc | Liquid detergents comprising lipase and bleach catalyst |
EP2607468A1 (en) | 2011-12-20 | 2013-06-26 | Henkel AG & Co. KGaA | Detergent compositions comprising subtilase variants |
WO2013092052A1 (en) | 2011-12-20 | 2013-06-27 | Unilever Plc | Isotropic liquid detergents comprising soil release polymer |
MX2014007446A (es) | 2011-12-20 | 2014-08-01 | Novozymes As | Variantes de subtilasa y polinucleotidos que las codifican. |
EP2794866A1 (en) | 2011-12-22 | 2014-10-29 | Danisco US Inc. | Compositions and methods comprising a lipolytic enzyme variant |
CA2858252A1 (en) | 2011-12-22 | 2013-06-27 | Danisco Us Inc. | Variant alpha-amylases and methods of use, thereof |
US10174301B2 (en) | 2011-12-28 | 2019-01-08 | Novozymes A/S | Methods for improving the nutritional value of the animal feed using a protease |
CN111187676A (zh) | 2011-12-29 | 2020-05-22 | 诺维信公司 | 具有脂肪酶变体的洗涤剂组合物 |
US9801398B2 (en) | 2012-01-26 | 2017-10-31 | Novozymes A/S | Use of polypeptides having protease activity in animal feed and detergents |
ES2582608T3 (es) | 2012-02-17 | 2016-09-14 | Henkel Ag & Co. Kgaa | Composiciones detergentes que comprenden variantes de subtilasa |
MX350713B (es) | 2012-02-17 | 2017-09-14 | Novozymes As | Variantes de subtilisina y polinucleotidos que las codifican. |
US20150064773A1 (en) | 2012-03-07 | 2015-03-05 | Novozymes A/S | Detergent Composition and Substitution of Optical Brighteners in Detergent Composition |
EP2639291A1 (en) | 2012-03-13 | 2013-09-18 | Unilever PLC | Packaged particulate detergent composition |
US10087401B2 (en) | 2012-03-16 | 2018-10-02 | Monosol, Llc | Water soluble compositions incorporating enzymes, and method of making same |
WO2013139702A1 (en) | 2012-03-21 | 2013-09-26 | Unilever Plc | Laundry detergent particles |
CN104204183A (zh) | 2012-03-29 | 2014-12-10 | 诺维信公司 | 酶用于制备水溶性膜的用途 |
WO2013149858A1 (en) | 2012-04-02 | 2013-10-10 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
EP2834336B1 (en) | 2012-04-03 | 2019-09-11 | Unilever PLC, a company registered in England and Wales under company no. 41424 | Laundry detergent particles |
BR112014020539B1 (pt) | 2012-04-03 | 2021-10-05 | Unilever Ip Holdings B.V. | Partícula detergente revestida e formulação detergente embalada |
CA2866963C (en) | 2012-04-03 | 2020-04-07 | Unilever Plc | Laundry detergent particles |
CN104220582B (zh) | 2012-04-03 | 2017-12-22 | 荷兰联合利华有限公司 | 洗衣洗涤剂颗粒 |
US9394092B2 (en) | 2012-04-16 | 2016-07-19 | Monosol, Llc | Powdered pouch and method of making same |
BR112014026433A2 (pt) | 2012-04-23 | 2017-06-27 | Unilever Nv | composição detergente líquida aquosa estruturada, composição de alta formação de espuma e processo para fabricar um líquido detergente estruturado com fibra de maçã sem polpa |
CN104245926B (zh) | 2012-04-27 | 2021-05-07 | 诺维信股份有限公司 | Gh61多肽变体以及编码其的多核苷酸 |
AR090971A1 (es) | 2012-05-07 | 2014-12-17 | Novozymes As | Polipeptidos que tienen actividad de degradacion de xantano y polinucleotidos que los codifican |
CA2870830A1 (en) | 2012-05-11 | 2013-11-14 | Danisco Us Inc. | Use of alpha-amylase from aspergillus clavatus for saccharification |
JP2015525248A (ja) | 2012-05-16 | 2015-09-03 | ノボザイムス アクティーゼルスカブ | リパーゼを含む組成物およびその使用方法 |
HUE057894T2 (hu) | 2012-06-08 | 2022-06-28 | Danisco Us Inc | Alfa-amiláz változatok javított aktivitással keményítõpolimereken |
US20150184208A1 (en) | 2012-06-19 | 2015-07-02 | Novozymes A/S | Enzymatic reduction of hydroperoxides |
MX364390B (es) | 2012-06-20 | 2019-04-25 | Novozymes As | Uso de polipeptidos que tienen actividad proteasa en alimentos para animales y detergentes. |
US10246692B2 (en) * | 2012-07-12 | 2019-04-02 | Novozymes A/S | Polypeptides having lipase activity and polynucleotides encoding same |
WO2014012506A1 (en) | 2012-07-18 | 2014-01-23 | Novozymes A/S | Method of treating polyester textile |
PL3553172T3 (pl) | 2012-08-16 | 2023-03-27 | Novozymes A/S | Sposób obróbki wyrobu włókienniczego z użyciem endoglukanazy |
US20150218606A1 (en) | 2012-08-16 | 2015-08-06 | Danisco Us Inc. | Method of using alpha-amylase from aspergillus clavatus and pullulanase for saccharification |
CN104603266B (zh) | 2012-08-22 | 2017-11-10 | 诺维信公司 | 来自脂环酸芽孢杆菌属的金属蛋白酶 |
WO2014029820A1 (en) | 2012-08-22 | 2014-02-27 | Novozymes A/S | Detergent compositions comprising metalloproteases |
BR112015003724A2 (pt) | 2012-08-22 | 2017-08-08 | Novozymes As | polipeptídeo isolado, composição, uso de uma composição, polinucleotídeo isolado, constructo de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, e, métodos de produção de um polipeptídeo e de produção de uma proteína. |
MX346181B (es) | 2012-09-25 | 2017-03-10 | Unilever Nv | Particulas detergentes para lavado de ropa. |
CN102863629A (zh) * | 2012-09-28 | 2013-01-09 | 南开大学 | 一种具有良好热稳定性的脂肪酶微乳液的制备方法 |
WO2014068083A1 (en) | 2012-11-01 | 2014-05-08 | Novozymes A/S | Method for removal of dna |
US20180112203A1 (en) | 2012-11-20 | 2018-04-26 | Danisco Us Inc. | Amylase with maltogenic properties |
US10323217B2 (en) | 2012-12-07 | 2019-06-18 | Novozymes A/S | Detergent composition comprising enzymes and washing method for preventing adhesion of bacteria |
EP3321353A1 (en) | 2012-12-11 | 2018-05-16 | Danisco US Inc. | Yeast host cells epxressing a glucoamylase from aspergillus fumigatus and methods of use thereof |
WO2014090940A1 (en) | 2012-12-14 | 2014-06-19 | Novozymes A/S | Removal of skin-derived body soils |
CA2894513A1 (en) | 2012-12-14 | 2014-06-19 | Danisco Us Inc. | Method of using alpha-amylase from aspergillus fumigatus and isoamylase for saccharification |
EP2904105A1 (en) | 2012-12-20 | 2015-08-12 | Danisco US Inc. | Method of using alpha-amylase from aspergillus terreus and pullulanase for saccharification |
WO2014099525A1 (en) | 2012-12-21 | 2014-06-26 | Danisco Us Inc. | Paenibacillus curdlanolyticus amylase, and methods of use, thereof |
CN104869841A (zh) | 2012-12-21 | 2015-08-26 | 诺维信公司 | 具有蛋白酶活性的多肽和编码它的多核苷酸 |
EP3354728B1 (en) | 2012-12-21 | 2020-04-22 | Danisco US Inc. | Alpha-amylase variants |
US9902946B2 (en) | 2013-01-03 | 2018-02-27 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
EP2767579B1 (en) | 2013-02-19 | 2018-07-18 | The Procter and Gamble Company | Method of laundering a fabric |
ES2834373T3 (es) | 2013-02-19 | 2021-06-17 | Procter & Gamble | Método para lavado de un tejido |
BR112015021647B1 (pt) | 2013-03-11 | 2023-04-25 | Danisco Us Inc | Variantes recombinantes de alfa-amilase e composições de detergente compreendendo as mesmas |
US20160024440A1 (en) | 2013-03-14 | 2016-01-28 | Novozymes A/S | Enzyme and Inhibitor Containing Water-Soluble Films |
CN105051174B (zh) | 2013-03-21 | 2018-04-03 | 诺维信公司 | 具有脂肪酶活性的多肽和编码它们的多核苷酸 |
CN105164147B (zh) | 2013-04-23 | 2020-03-03 | 诺维信公司 | 具有稳定的枯草杆菌蛋白酶的液体自动餐具清洗洗涤剂组合物 |
WO2014177709A1 (en) | 2013-05-03 | 2014-11-06 | Novozymes A/S | Microencapsulation of detergent enzymes |
BR112015028666B8 (pt) | 2013-05-14 | 2022-08-09 | Novozymes As | Composição detergente, método para produzir a mesma, método para a limpeza de um objeto e usos da composição |
US20160083703A1 (en) | 2013-05-17 | 2016-03-24 | Novozymes A/S | Polypeptides having alpha amylase activity |
CN114634921A (zh) | 2013-06-06 | 2022-06-17 | 诺维信公司 | α-淀粉酶变体以及对其进行编码的多核苷酸 |
US20160130489A1 (en) | 2013-06-12 | 2016-05-12 | Novozymes A/S | Dust suppressant |
WO2014200658A1 (en) | 2013-06-13 | 2014-12-18 | Danisco Us Inc. | Alpha-amylase from promicromonospora vindobonensis |
WO2014200656A1 (en) | 2013-06-13 | 2014-12-18 | Danisco Us Inc. | Alpha-amylase from streptomyces umbrinus |
WO2014200657A1 (en) | 2013-06-13 | 2014-12-18 | Danisco Us Inc. | Alpha-amylase from streptomyces xiamenensis |
WO2014204596A1 (en) | 2013-06-17 | 2014-12-24 | Danisco Us Inc. | Alpha-amylase from bacillaceae family member |
WO2014207227A1 (en) | 2013-06-27 | 2014-12-31 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
WO2014207224A1 (en) | 2013-06-27 | 2014-12-31 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
CN105358670A (zh) | 2013-07-04 | 2016-02-24 | 诺维信公司 | 具有抗再沉积效果的具黄原胶裂解酶活性的多肽与编码它们的多核苷酸 |
EP3019603A1 (en) | 2013-07-09 | 2016-05-18 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
EP3022299B1 (en) | 2013-07-19 | 2020-03-18 | Danisco US Inc. | Compositions and methods comprising a lipolytic enzyme variant |
RU2670946C9 (ru) | 2013-07-29 | 2018-11-26 | Новозимс А/С | Варианты протеазы и кодирующие их полинуклеотиды |
EP3339436B1 (en) | 2013-07-29 | 2021-03-31 | Henkel AG & Co. KGaA | Detergent composition comprising protease variants |
WO2015014803A1 (en) | 2013-07-29 | 2015-02-05 | Novozymes A/S | Protease variants and polynucleotides encoding same |
US20160160199A1 (en) | 2013-10-03 | 2016-06-09 | Danisco Us Inc. | Alpha-amylases from exiguobacterium, and methods of use, thereof |
WO2015049370A1 (en) | 2013-10-03 | 2015-04-09 | Novozymes A/S | Detergent composition and use of detergent composition |
US20160186102A1 (en) | 2013-10-03 | 2016-06-30 | Danisco Us Inc. | Alpha-amylases from exiguobacterium, and methods of use, thereof |
EP3071691B1 (en) | 2013-11-20 | 2019-10-23 | Danisco US Inc. | Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof |
WO2015095046A1 (en) | 2013-12-16 | 2015-06-25 | E. I. Du Pont De Nemours And Company | Use of poly alpha-1,3-glucan ethers as viscosity modifiers |
EP3083705B1 (en) | 2013-12-18 | 2020-09-30 | DuPont Industrial Biosciences USA, LLC | Cationic poly alpha-1,3-glucan ethers |
WO2015094809A1 (en) | 2013-12-19 | 2015-06-25 | Danisco Us Inc. | Chimeric fungal alpha-amylases comprising carbohydrate binding module and the use thereof |
EP3453757B1 (en) | 2013-12-20 | 2020-06-17 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2015109972A1 (en) | 2014-01-22 | 2015-07-30 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
EP3105256A1 (en) | 2014-02-14 | 2016-12-21 | E. I. du Pont de Nemours and Company | Poly-alpha-1,3-1,6-glucans for viscosity modification |
EP3114272A1 (en) | 2014-03-05 | 2017-01-11 | Novozymes A/S | Compositions and methods for improving properties of cellulosic textile materials with xyloglucan endotransglycosylase |
EP3114219A1 (en) | 2014-03-05 | 2017-01-11 | Novozymes A/S | Compositions and methods for improving properties of non-cellulosic textile materials with xyloglucan endotransglycosylase |
ES2734726T3 (es) | 2014-03-11 | 2019-12-11 | Du Pont | Poli alfa-1,2-glucano oxidado como reforzante de detergente |
CN106103721B (zh) | 2014-03-12 | 2020-01-03 | 诺维信公司 | 具有脂肪酶活性的多肽和编码它们的多核苷酸 |
CN106103708A (zh) | 2014-04-01 | 2016-11-09 | 诺维信公司 | 具有α淀粉酶活性的多肽 |
US10131863B2 (en) | 2014-04-11 | 2018-11-20 | Novozymes A/S | Detergent composition |
WO2015158237A1 (en) | 2014-04-15 | 2015-10-22 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
WO2015181119A2 (en) | 2014-05-27 | 2015-12-03 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
EP3878957A1 (en) | 2014-05-27 | 2021-09-15 | Novozymes A/S | Methods for producing lipases |
CN106414729A (zh) | 2014-06-12 | 2017-02-15 | 诺维信公司 | α‑淀粉酶变体以及对其进行编码的多核苷酸 |
EP3158043B1 (en) | 2014-06-19 | 2021-03-10 | Nutrition & Biosciences USA 4, Inc. | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
US9714403B2 (en) | 2014-06-19 | 2017-07-25 | E I Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
US20170121646A1 (en) | 2014-07-03 | 2017-05-04 | Novozymes A/S | Improved Stabilization of Non-Protease Enzyme |
EP3878960A1 (en) | 2014-07-04 | 2021-09-15 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
WO2016001450A2 (en) | 2014-07-04 | 2016-01-07 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
EP2987849A1 (en) | 2014-08-19 | 2016-02-24 | The Procter and Gamble Company | Method of Laundering a Fabric |
EP2987848A1 (en) | 2014-08-19 | 2016-02-24 | The Procter & Gamble Company | Method of laundering a fabric |
WO2016041676A1 (en) | 2014-09-18 | 2016-03-24 | Unilever Plc | Whitening composition |
WO2016079110A2 (en) | 2014-11-19 | 2016-05-26 | Novozymes A/S | Use of enzyme for cleaning |
US10287562B2 (en) | 2014-11-20 | 2019-05-14 | Novoszymes A/S | Alicyclobacillus variants and polynucleotides encoding same |
WO2016087617A1 (en) | 2014-12-04 | 2016-06-09 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
CN116286218A (zh) | 2014-12-04 | 2023-06-23 | 诺维信公司 | 包括蛋白酶变体的液体清洁组合物 |
WO2016087401A1 (en) | 2014-12-05 | 2016-06-09 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
WO2016096714A1 (en) | 2014-12-15 | 2016-06-23 | Henkel Ag & Co. Kgaa | Detergent composition comprising subtilase variants |
US20180000076A1 (en) | 2014-12-16 | 2018-01-04 | Novozymes A/S | Polypeptides Having N-Acetyl Glucosamine Oxidase Activity |
DK3234093T3 (da) | 2014-12-19 | 2020-08-17 | Novozymes As | Proteasevarianter og polynukleotider, som koder for dem |
WO2016097350A1 (en) | 2014-12-19 | 2016-06-23 | Novozymes A/S | Protease variants and polynucleotides encoding same |
US20180334696A1 (en) | 2014-12-23 | 2018-11-22 | E I Du Pont De Nemours And Company | Enzymatically produced cellulose |
BR112017014650B1 (pt) | 2015-01-08 | 2022-10-11 | Stepan Company | Detergente de vestuário útil para limpeza com água fria compreendendo um surfactante com ponte alquileno e uma lipase e método para lavar um artigo têxtil sujo em água |
EP3242927B1 (en) | 2015-01-09 | 2018-10-10 | Unilever PLC, a company registered in England and Wales under company no. 41424 | Laundry treatment composition comprising a dye |
CN107207998B (zh) | 2015-02-13 | 2020-04-10 | 荷兰联合利华有限公司 | 液体洗衣组合物 |
WO2016133734A1 (en) | 2015-02-18 | 2016-08-25 | E. I. Du Pont De Nemours And Company | Soy polysaccharide ethers |
WO2016160407A1 (en) | 2015-03-31 | 2016-10-06 | Stepan Company | Detergents based on alpha-sulfonated fatty ester surfactants |
BR112017019942A2 (pt) | 2015-04-02 | 2018-06-12 | Unilever Nv | composição líquida para lavagem de roupas e uso de polímero para a liberação de sujeira |
CN107864658A (zh) | 2015-04-07 | 2018-03-30 | 诺维信公司 | 用于选择具有脂肪酶活性的酶的方法 |
US20180105772A1 (en) | 2015-04-10 | 2018-04-19 | Novozymes A/S | Detergent composition |
WO2016162556A1 (en) | 2015-04-10 | 2016-10-13 | Novozymes A/S | Laundry method, use of dnase and detergent composition |
WO2016184944A1 (en) | 2015-05-19 | 2016-11-24 | Novozymes A/S | Odor reduction |
ES2712459T3 (es) | 2015-06-04 | 2019-05-13 | Procter & Gamble | Composición detergente líquida para lavado de vajillas a mano |
ES2665989T3 (es) | 2015-06-04 | 2018-04-30 | The Procter & Gamble Company | Composición detergente líquida para lavado de vajillas a mano |
TR201906232T4 (tr) | 2015-06-11 | 2019-05-21 | Unilever Nv | Çamaşır için deterjan bileşimi. |
EP3310908B1 (en) | 2015-06-16 | 2020-08-05 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
WO2016202785A1 (en) | 2015-06-17 | 2016-12-22 | Novozymes A/S | Container |
EP3106508B1 (en) | 2015-06-18 | 2019-11-20 | Henkel AG & Co. KGaA | Detergent composition comprising subtilase variants |
CN108012544A (zh) | 2015-06-18 | 2018-05-08 | 诺维信公司 | 枯草杆菌酶变体以及编码它们的多核苷酸 |
TR201815258T4 (tr) | 2015-06-26 | 2018-11-21 | Unilever Nv | Çamaşır yıkama deterjanı bileşimi. |
EP3313966B1 (en) | 2015-06-26 | 2020-07-29 | Unilever PLC | Laundry detergent composition |
EP3317388B1 (en) | 2015-06-30 | 2019-11-13 | Novozymes A/S | Laundry detergent composition, method for washing and use of composition |
EP3929285A3 (en) | 2015-07-01 | 2022-05-25 | Novozymes A/S | Methods of reducing odor |
EP3320089B1 (en) | 2015-07-06 | 2021-06-16 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
TR201900475T4 (tr) | 2015-08-28 | 2019-02-21 | Unilever Nv | Lipaz Ve Proteaz İçeren Sıvı Deterjan Bileşimi |
CA2991114A1 (en) | 2015-09-17 | 2017-03-23 | Novozymes A/S | Polypeptides having xanthan degrading activity and polynucleotides encoding same |
KR20180053365A (ko) | 2015-09-17 | 2018-05-21 | 헨켈 아게 운트 코. 카게아아 | 크산탄 분해 활성을 갖는 폴리펩티드를 포함하는 세제 조성물 |
WO2017050441A1 (en) | 2015-09-24 | 2017-03-30 | Unilever N.V. | Substrate washing device |
US10934535B2 (en) | 2015-10-07 | 2021-03-02 | Novozymes A/S | Detergent composition comprising a DNase |
CN113913475A (zh) | 2015-10-09 | 2022-01-11 | 诺维信公司 | 酶或非酶生物柴油精制方法 |
EP3362168A1 (en) | 2015-10-14 | 2018-08-22 | Novozymes A/S | Cleaning of water filtration membranes |
CN108291212A (zh) | 2015-10-14 | 2018-07-17 | 诺维信公司 | 多肽变体 |
CN108291215A (zh) | 2015-10-14 | 2018-07-17 | 诺维信公司 | 具有蛋白酶活性的多肽以及编码它们的多核苷酸 |
WO2016203064A2 (en) | 2015-10-28 | 2016-12-22 | Novozymes A/S | Detergent composition comprising protease and amylase variants |
EP3374488B1 (en) | 2015-11-13 | 2020-10-14 | DuPont Industrial Biosciences USA, LLC | Glucan fiber compositions for use in laundry care and fabric care |
JP7045313B2 (ja) | 2015-11-13 | 2022-03-31 | ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー,インコーポレイテッド | 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物 |
JP2019504932A (ja) | 2015-11-13 | 2019-02-21 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーE.I.Du Pont De Nemours And Company | 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物 |
US11001821B2 (en) | 2015-11-24 | 2021-05-11 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
CN108431217B (zh) | 2015-12-01 | 2022-06-21 | 诺维信公司 | 用于产生脂肪酶的方法 |
PL3387125T3 (pl) | 2015-12-07 | 2023-01-09 | Henkel Ag & Co. Kgaa | Kompozycje do mycia naczyń zawierające polipeptydy posiadające aktywność beta-glukanazy oraz ich zastosowania |
WO2017100720A1 (en) | 2015-12-09 | 2017-06-15 | Danisco Us Inc. | Alpha-amylase combinatorial variants |
CN108367245A (zh) | 2015-12-09 | 2018-08-03 | 巴斯夫欧洲公司 | 在解吸条件下从发酵固体纯化蛋白质的方法 |
EP3397061A1 (en) | 2015-12-28 | 2018-11-07 | Novozymes BioAG A/S | Heat priming of bacterial spores |
WO2017114891A1 (en) | 2015-12-30 | 2017-07-06 | Novozymes A/S | Enzyme variants and polynucleotides encoding the same |
TR201808208T4 (tr) | 2016-01-07 | 2018-07-23 | Unilever Nv | Acı parçacık. |
CN108473920B (zh) | 2016-01-15 | 2020-03-10 | 荷兰联合利华有限公司 | 染料 |
WO2017129754A1 (en) | 2016-01-29 | 2017-08-03 | Novozymes A/S | Beta-glucanase variants and polynucleotides encoding same |
WO2017133879A1 (en) | 2016-02-04 | 2017-08-10 | Unilever Plc | Detergent liquid |
CN108603139B (zh) | 2016-02-17 | 2020-12-04 | 荷兰联合利华有限公司 | 增白组合物 |
BR112018016674B1 (pt) | 2016-02-17 | 2022-06-07 | Unilever Ip Holdings B.V. | Composição de detergente para lavagem de roupas e método doméstico de tratamento de um tecido |
US10221379B2 (en) | 2016-02-26 | 2019-03-05 | The Procter & Gamble Company | Thickened or structured liquid detergent compositions |
WO2017162378A1 (en) | 2016-03-21 | 2017-09-28 | Unilever Plc | Laundry detergent composition |
EP3715442A1 (en) | 2016-03-23 | 2020-09-30 | Novozymes A/S | Use of polypeptide having dnase activity for treating fabrics |
WO2017173324A2 (en) | 2016-04-01 | 2017-10-05 | Danisco Us Inc. | Alpha-amylases, compositions & methods |
WO2017173190A2 (en) | 2016-04-01 | 2017-10-05 | Danisco Us Inc. | Alpha-amylases, compositions & methods |
CN108779416B (zh) | 2016-04-08 | 2021-01-05 | 荷兰联合利华有限公司 | 洗衣洗涤剂组合物 |
US20200325418A1 (en) | 2016-04-08 | 2020-10-15 | Novozymes A/S | Detergent compositions and uses of the same |
EP3693449A1 (en) | 2016-04-29 | 2020-08-12 | Novozymes A/S | Detergent compositions and uses thereof |
WO2017191160A1 (en) | 2016-05-03 | 2017-11-09 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding the same |
CA3022121A1 (en) | 2016-05-09 | 2017-11-16 | Novozymes A/S | Variant polypeptides with improved performance and use of the same |
WO2017210188A1 (en) | 2016-05-31 | 2017-12-07 | Novozymes A/S | Stabilized liquid peroxide compositions |
CN109715792A (zh) | 2016-06-03 | 2019-05-03 | 诺维信公司 | 枯草杆菌酶变体和对其进行编码的多核苷酸 |
EP3469048A1 (en) | 2016-06-09 | 2019-04-17 | Unilever PLC | Laundry products |
EP3475404A1 (en) | 2016-06-23 | 2019-05-01 | Novozymes A/S | Use of enzymes, composition and method for removing soil |
BR112018077510A2 (pt) | 2016-06-30 | 2019-04-02 | Novozymes As | variantes de lipase e composições que compreendem tensoativo e variante de lipase |
WO2018002261A1 (en) | 2016-07-01 | 2018-01-04 | Novozymes A/S | Detergent compositions |
JP2019522988A (ja) | 2016-07-05 | 2019-08-22 | ノボザイムス アクティーゼルスカブ | ペクチン酸リアーゼ変異体およびこれをコードするポリヌクレオチド |
WO2018007573A1 (en) | 2016-07-08 | 2018-01-11 | Novozymes A/S | Detergent compositions with galactanase |
JP6858850B2 (ja) | 2016-07-13 | 2021-04-14 | ザ プロクター アンド ギャンブル カンパニーThe Procter & Gamble Company | バチルス・シビ(BACILLUS CIBI)DNase変異体及びその使用 |
BR112018077483A2 (pt) | 2016-07-14 | 2019-04-02 | Basf Se | meio de fermentação, métodos para cultivar um microrganismo e para produzir um composto de interesse, solução de oligoelemento para um processo de fermentação, e, uso de um agente quelante. |
US11326152B2 (en) | 2016-07-18 | 2022-05-10 | Novozymes A/S | Lipase variants, polynucleotides encoding same and the use thereof |
ES2790148T3 (es) | 2016-08-17 | 2020-10-27 | Procter & Gamble | Composición limpiadora que comprende enzimas |
AU2017317564B2 (en) | 2016-08-24 | 2021-09-30 | Henkel Ag & Co. Kgaa | Detergent composition comprising GH9 endoglucanase variants I |
CA3032248A1 (en) | 2016-08-24 | 2018-03-01 | Novozymes A/S | Xanthan lyase variants and polynucleotides encoding same |
US11072765B2 (en) | 2016-08-24 | 2021-07-27 | Novozymes A/S | GH9 endoglucanase variants and polynucleotides encoding same |
AU2017317563B8 (en) | 2016-08-24 | 2023-03-23 | Henkel Ag & Co. Kgaa | Detergent compositions comprising xanthan lyase variants I |
CN110023474A (zh) | 2016-09-29 | 2019-07-16 | 诺维信公司 | 酶用于洗涤的用途、洗涤方法和器皿洗涤组合物 |
WO2018060475A1 (en) | 2016-09-29 | 2018-04-05 | Novozymes A/S | Spore containing granule |
CN109844083B (zh) | 2016-10-18 | 2021-11-09 | 联合利华知识产权控股有限公司 | 增白组合物 |
US20210284933A1 (en) | 2016-10-25 | 2021-09-16 | Novozymes A/S | Detergent compositions |
CN110072986B (zh) | 2016-11-01 | 2023-04-04 | 诺维信公司 | 多核心颗粒 |
CA3043443A1 (en) | 2016-12-01 | 2018-06-07 | Basf Se | Stabilization of enzymes in compositions |
EP3551740B1 (en) | 2016-12-12 | 2021-08-11 | Novozymes A/S | Use of polypeptides |
BR112019011999B1 (pt) | 2016-12-15 | 2022-11-08 | Unilever Ip Holdings B.V | Composição de detergente líquida aquosa para lavagem de roupas e método doméstico de tratamento de um tecido |
CN110651039A (zh) | 2017-03-31 | 2020-01-03 | 诺维信公司 | 具有rna酶活性的多肽 |
EP3601553A1 (en) | 2017-03-31 | 2020-02-05 | Danisco US Inc. | Alpha-amylase combinatorial variants |
WO2018177936A1 (en) | 2017-03-31 | 2018-10-04 | Novozymes A/S | Polypeptides having dnase activity |
EP3601550A1 (en) | 2017-03-31 | 2020-02-05 | Novozymes A/S | Polypeptides having dnase activity |
US11339355B2 (en) | 2017-04-04 | 2022-05-24 | Novozymes A/S | Glycosyl hydrolases |
EP3607040A1 (en) | 2017-04-04 | 2020-02-12 | Novozymes A/S | Polypeptide compositions and uses thereof |
EP3607039A1 (en) | 2017-04-04 | 2020-02-12 | Novozymes A/S | Polypeptides |
EP3385362A1 (en) | 2017-04-05 | 2018-10-10 | Henkel AG & Co. KGaA | Detergent compositions comprising fungal mannanases |
EP3385361B1 (en) | 2017-04-05 | 2019-03-27 | Henkel AG & Co. KGaA | Detergent compositions comprising bacterial mannanases |
WO2018184816A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
CN110662829B (zh) | 2017-04-06 | 2022-03-01 | 诺维信公司 | 清洁组合物及其用途 |
EP3607042A1 (en) | 2017-04-06 | 2020-02-12 | Novozymes A/S | Cleaning compositions and uses thereof |
MX2019011764A (es) | 2017-04-06 | 2019-11-28 | Novozymes As | Composiciones limpiadoras y usos de las mismas. |
WO2018184818A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
US11407964B2 (en) | 2017-04-06 | 2022-08-09 | Novozymes A/S | Cleaning compositions and uses thereof |
EP3607060B1 (en) | 2017-04-06 | 2021-08-11 | Novozymes A/S | Detergent compositions and uses thereof |
US20200190438A1 (en) | 2017-04-06 | 2020-06-18 | Novozymes A/S | Cleaning compositions and uses thereof |
CN110651038A (zh) | 2017-05-05 | 2020-01-03 | 诺维信公司 | 包含脂肪酶和亚硫酸盐的组合物 |
US20210130743A1 (en) | 2017-05-08 | 2021-05-06 | Novozymes A/S | Mannanase Variants and Polynucleotides Encoding Same |
EP3401385A1 (en) | 2017-05-08 | 2018-11-14 | Henkel AG & Co. KGaA | Detergent composition comprising polypeptide comprising carbohydrate-binding domain |
US11492605B2 (en) | 2017-05-08 | 2022-11-08 | Novozymes A/S | Mannanase variants and polynucleotides encoding same |
WO2018206535A1 (en) | 2017-05-08 | 2018-11-15 | Novozymes A/S | Carbohydrate-binding domain and polynucleotides encoding the same |
WO2018224544A1 (en) | 2017-06-08 | 2018-12-13 | Novozymes A/S | Compositions comprising polypeptides having cellulase activity and amylase activity, and uses thereof in cleaning and detergent compositions |
EP3635166A1 (en) | 2017-06-09 | 2020-04-15 | Unilever PLC, a company registered in England and Wales under company no. 41424 | Laundry liquid dispensing system |
WO2018234056A1 (en) | 2017-06-20 | 2018-12-27 | Unilever N.V. | PARTICULATE DETERGENT COMPOSITION COMPRISING A FRAGRANCE |
WO2018234003A1 (en) | 2017-06-21 | 2018-12-27 | Unilever Plc | PACKAGING AND DISTRIBUTION OF DETERGENT COMPOSITIONS |
CN111108183A (zh) | 2017-06-30 | 2020-05-05 | 诺维信公司 | 酶浆液组合物 |
BR112020000205B1 (pt) | 2017-07-07 | 2023-10-31 | Unilever Ip Holdings B.V. | Composição de limpeza para a lavagem de tecidos e método doméstico de tratamento de um tecido |
CN110869480B (zh) | 2017-07-07 | 2021-08-13 | 联合利华知识产权控股有限公司 | 增白组合物 |
CN111032856A (zh) | 2017-08-07 | 2020-04-17 | 诺维信公司 | 基于pH的FCA控制的用途 |
WO2019036721A2 (en) | 2017-08-18 | 2019-02-21 | Danisco Us Inc | VARIANTS OF ALPHA-AMYLASES |
WO2019038186A1 (en) | 2017-08-24 | 2019-02-28 | Unilever Plc | IMPROVEMENTS RELATING TO THE CLEANING OF FABRICS |
WO2019038060A1 (en) | 2017-08-24 | 2019-02-28 | Henkel Ag & Co. Kgaa | DETERGENT COMPOSITION COMPRISING XANTHANE LYASE II VARIANTS |
CN111278971A (zh) | 2017-08-24 | 2020-06-12 | 诺维信公司 | Gh9内切葡聚糖酶变体以及编码它们的多核苷酸 |
US11624059B2 (en) | 2017-08-24 | 2023-04-11 | Henkel Ag & Co. Kgaa | Detergent compositions comprising GH9 endoglucanase variants II |
WO2019038187A1 (en) | 2017-08-24 | 2019-02-28 | Unilever Plc | IMPROVEMENTS RELATING TO THE CLEANING OF FABRICS |
US11359188B2 (en) | 2017-08-24 | 2022-06-14 | Novozymes A/S | Xanthan lyase variants and polynucleotides encoding same |
MX2020002953A (es) | 2017-09-20 | 2020-07-22 | Novozymes As | Uso de enzimas para mejorar la absorcion de agua y/o la blancura. |
US11414814B2 (en) | 2017-09-22 | 2022-08-16 | Novozymes A/S | Polypeptides |
MX2020002964A (es) | 2017-09-27 | 2020-07-22 | Novozymes As | Variantes de lipasa y composiciones de microcapsula que comprenden las variantes de lipasa. |
JP7114697B2 (ja) | 2017-09-27 | 2022-08-08 | ザ プロクター アンド ギャンブル カンパニー | リパーゼを含む洗剤組成物 |
CN111417725A (zh) | 2017-10-02 | 2020-07-14 | 诺维信公司 | 具有甘露聚糖酶活性的多肽和编码它们的多核苷酸 |
WO2019068715A1 (en) | 2017-10-02 | 2019-04-11 | Novozymes A/S | POLYPEPTIDES HAVING MANNANASE ACTIVITY AND POLYNUCLEOTIDES ENCODING THESE POLYPEPTIDES |
CN111448302A (zh) | 2017-10-16 | 2020-07-24 | 诺维信公司 | 低粉化颗粒 |
WO2019076800A1 (en) | 2017-10-16 | 2019-04-25 | Novozymes A/S | CLEANING COMPOSITIONS AND USES THEREOF |
WO2019076834A1 (en) | 2017-10-16 | 2019-04-25 | Novozymes A/S | PELLETS WITH LOW DUST CONTENT |
US11866748B2 (en) | 2017-10-24 | 2024-01-09 | Novozymes A/S | Compositions comprising polypeptides having mannanase activity |
WO2019081724A1 (en) | 2017-10-27 | 2019-05-02 | Novozymes A/S | VARIANTS OF DNASE |
PL3476935T3 (pl) | 2017-10-27 | 2022-03-28 | The Procter & Gamble Company | Kompozycje detergentowe zawierające odmiany polipeptydowe |
US11505767B2 (en) | 2017-11-01 | 2022-11-22 | Novozymes A/S | Methods for cleansing medical devices |
US11767492B2 (en) | 2017-11-01 | 2023-09-26 | Novozymes A/S | Methods of treating fabric using a Lactobacillus hexosaminidase |
DE102017125559A1 (de) | 2017-11-01 | 2019-05-02 | Henkel Ag & Co. Kgaa | Reinigungszusammensetzungen, die dispersine ii enthalten |
DE102017125560A1 (de) | 2017-11-01 | 2019-05-02 | Henkel Ag & Co. Kgaa | Reinigungszusammensetzungen, die dispersine iii enthalten |
DE102017125558A1 (de) | 2017-11-01 | 2019-05-02 | Henkel Ag & Co. Kgaa | Reinigungszusammensetzungen, die dispersine i enthalten |
WO2019086528A1 (en) | 2017-11-01 | 2019-05-09 | Novozymes A/S | Polypeptides and compositions comprising such polypeptides |
MX2020004831A (es) | 2017-11-09 | 2020-10-16 | Basf Se | Recubrimientos de particulas de enzima que comprenden pigmentos blancos organicos. |
BR112020009317A2 (pt) | 2017-11-13 | 2020-10-27 | Unilever N.V. | método para demonstrar a remoção de sebo em tecidos lavados por uma ou mais composições de detergente para lavanderia |
WO2019101951A1 (en) | 2017-11-24 | 2019-05-31 | Novozymes A/S | Small enzyme particles for interesterification |
CA3083390A1 (en) | 2017-11-29 | 2019-06-06 | Basf Se | Storage-stable enzyme preparations, their production and use |
WO2019105675A1 (en) | 2017-11-30 | 2019-06-06 | Unilever Plc | Detergent composition comprising protease |
WO2019110462A1 (en) | 2017-12-04 | 2019-06-13 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
JP7309718B2 (ja) | 2017-12-20 | 2023-07-18 | ビーエーエスエフ ソシエタス・ヨーロピア | 繊維製品に付着した、融解温度が30℃超のである脂肪化合物を除去するための洗濯用配合物 |
US20210123033A1 (en) | 2018-02-08 | 2021-04-29 | Novozymes A/S | Lipases, Lipase Variants and Compositions Thereof |
US20210071155A1 (en) | 2018-02-08 | 2021-03-11 | Novozymes A/S | Lipase Variants and Compositions Thereof |
EP3755793A1 (en) | 2018-02-23 | 2020-12-30 | Henkel AG & Co. KGaA | Detergent composition comprising xanthan lyase and endoglucanase variants |
WO2019175240A1 (en) | 2018-03-13 | 2019-09-19 | Novozymes A/S | Microencapsulation using amino sugar oligomers |
US20210009979A1 (en) | 2018-03-23 | 2021-01-14 | Novozymes A/S | Subtilase variants and compositions comprising same |
WO2019185726A1 (en) | 2018-03-29 | 2019-10-03 | Novozymes A/S | Mannanase variants and polynucleotides encoding same |
WO2019201793A1 (en) | 2018-04-17 | 2019-10-24 | Novozymes A/S | Polypeptides comprising carbohydrate binding activity in detergent compositions and their use in reducing wrinkles in textile or fabric. |
US11661592B2 (en) | 2018-04-19 | 2023-05-30 | Novozymes A/S | Stabilized endoglucanase variants |
EP3781680A1 (en) | 2018-04-19 | 2021-02-24 | Novozymes A/S | Stabilized cellulase variants |
CN111989389A (zh) | 2018-04-19 | 2020-11-24 | 巴斯夫欧洲公司 | 组合物以及可用于该组合物的聚合物 |
BR112020023123A2 (pt) | 2018-05-17 | 2021-02-02 | Unilever N.V. | composição de limpeza e método doméstico para tratar um tecido |
CN112119147B (zh) | 2018-05-17 | 2023-09-29 | 联合利华知识产权控股有限公司 | 清洁组合物 |
WO2019238761A1 (en) | 2018-06-15 | 2019-12-19 | Basf Se | Water soluble multilayer films containing wash active chemicals and enzymes |
WO2020002604A1 (en) | 2018-06-28 | 2020-01-02 | Novozymes A/S | Detergent compositions and uses thereof |
EP3814473A1 (en) | 2018-06-29 | 2021-05-05 | Novozymes A/S | Detergent compositions and uses thereof |
US20210189297A1 (en) | 2018-06-29 | 2021-06-24 | Novozymes A/S | Subtilase variants and compositions comprising same |
US20210189296A1 (en) | 2018-07-02 | 2021-06-24 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2020007875A1 (en) | 2018-07-03 | 2020-01-09 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2020008024A1 (en) | 2018-07-06 | 2020-01-09 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2020008043A1 (en) | 2018-07-06 | 2020-01-09 | Novozymes A/S | Cleaning compositions and uses thereof |
CN112513236A (zh) | 2018-07-17 | 2021-03-16 | 联合利华知识产权控股有限公司 | 鼠李糖脂在表面活性剂体系中的用途 |
WO2020020703A1 (en) | 2018-07-27 | 2020-01-30 | Unilever N.V. | Laundry detergent |
WO2020028443A1 (en) | 2018-07-31 | 2020-02-06 | Danisco Us Inc | Variant alpha-amylases having amino acid substitutions that lower the pka of the general acid |
WO2020030623A1 (en) | 2018-08-10 | 2020-02-13 | Basf Se | Packaging unit comprising a detergent composition containing an enzyme and at least one chelating agent |
WO2020058091A1 (en) | 2018-09-18 | 2020-03-26 | Unilever Plc | Method of chemical monitoring the fat removal from surfaces |
US20220033736A1 (en) | 2018-09-18 | 2022-02-03 | Conopco, Inc., D/B/A Unilever | Detergent composition |
US20210340466A1 (en) | 2018-10-01 | 2021-11-04 | Novozymes A/S | Detergent compositions and uses thereof |
WO2020070209A1 (en) | 2018-10-02 | 2020-04-09 | Novozymes A/S | Cleaning composition |
WO2020070014A1 (en) | 2018-10-02 | 2020-04-09 | Novozymes A/S | Cleaning composition comprising anionic surfactant and a polypeptide having rnase activity |
US20230287306A1 (en) | 2018-10-02 | 2023-09-14 | Novozymes A/S | Cleaning Composition |
WO2020070249A1 (en) | 2018-10-03 | 2020-04-09 | Novozymes A/S | Cleaning compositions |
WO2020070199A1 (en) | 2018-10-03 | 2020-04-09 | Novozymes A/S | Polypeptides having alpha-mannan degrading activity and polynucleotides encoding same |
WO2020069915A1 (en) | 2018-10-05 | 2020-04-09 | Basf Se | Compounds stabilizing hydrolases in liquids |
EP3677676A1 (en) | 2019-01-03 | 2020-07-08 | Basf Se | Compounds stabilizing amylases in liquids |
MX2021003931A (es) | 2018-10-05 | 2021-06-04 | Basf Se | Compuestos estabilizadores de amilasas en liquidos. |
BR112021005954A2 (pt) | 2018-10-05 | 2021-06-29 | Basf Se | preparação enzimática, processo para produzir uma preparação enzimática estável, métodos para reduzir perda de atividade lipolítica de pelo menos uma lipase, para preparar uma formulação de detergente, para remover manchas e para aumentar a estabilidade de armazenagem de uma formulação de detergente líquido, usos de um composto e da preparação enzimática, e, formulação de detergente. |
EP3864122A1 (en) | 2018-10-09 | 2021-08-18 | Novozymes A/S | Cleaning compositions and uses thereof |
EP3864123A1 (en) | 2018-10-09 | 2021-08-18 | Novozymes A/S | Cleaning compositions and uses thereof |
EP3864124A1 (en) | 2018-10-11 | 2021-08-18 | Novozymes A/S | Cleaning compositions and uses thereof |
US20210355469A1 (en) | 2018-10-12 | 2021-11-18 | Danisco Us Inc | Alpha-amylases with mutations that improve stability in the presence of chelants |
EP3647397A1 (en) | 2018-10-31 | 2020-05-06 | Henkel AG & Co. KGaA | Cleaning compositions containing dispersins iv |
EP3647398B1 (en) | 2018-10-31 | 2024-05-15 | Henkel AG & Co. KGaA | Cleaning compositions containing dispersins v |
WO2020104231A1 (en) | 2018-11-19 | 2020-05-28 | Basf Se | Powders and granules containing a chelating agent and an enzyme |
BR112021009789A2 (pt) | 2018-11-20 | 2021-08-17 | Unilever Ip Holdings B.V. | composição detergente, método de tratamento de um substrato de tecido e uso de uma enzima esterase |
WO2020104156A1 (en) | 2018-11-20 | 2020-05-28 | Unilever Plc | Detergent composition |
CN113056549B (zh) | 2018-11-20 | 2023-03-10 | 联合利华知识产权控股有限公司 | 洗涤剂组合物 |
BR112021009828A2 (pt) | 2018-11-20 | 2021-08-17 | Unilever Ip Holdings B.V. | composição detergente líquida, método de tratamento de um substrato de tecido e uso de uma enzima esterol esterase |
WO2020114965A1 (en) | 2018-12-03 | 2020-06-11 | Novozymes A/S | LOW pH POWDER DETERGENT COMPOSITION |
EP3891277A1 (en) | 2018-12-03 | 2021-10-13 | Novozymes A/S | Powder detergent compositions |
WO2020127796A2 (en) | 2018-12-21 | 2020-06-25 | Novozymes A/S | Polypeptides having peptidoglycan degrading activity and polynucleotides encoding same |
EP3898919A1 (en) | 2018-12-21 | 2021-10-27 | Novozymes A/S | Detergent pouch comprising metalloproteases |
BR112021014332A2 (pt) | 2019-01-22 | 2021-09-28 | Unilever Ip Holdings B.V. | Composição detergente de lavanderia, método doméstico para tratar um tecido e corante tonalizante não leuco azul ou violeta |
WO2020151992A1 (en) | 2019-01-22 | 2020-07-30 | Unilever N.V. | Laundry detergent |
EP3702452A1 (en) | 2019-03-01 | 2020-09-02 | Novozymes A/S | Detergent compositions comprising two proteases |
US20220177808A1 (en) | 2019-03-08 | 2022-06-09 | Basf Se | Cationic surfactant and its use in laundry detergent compositions |
EP3942032A1 (en) | 2019-03-21 | 2022-01-26 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2020193101A1 (en) | 2019-03-22 | 2020-10-01 | Unilever Plc | Method for washing a garment worn on the head |
CN113785039A (zh) | 2019-04-03 | 2021-12-10 | 诺维信公司 | 具有β-葡聚糖酶活性的多肽、编码其的多核苷酸及其在清洁和洗涤剂组合物中的用途 |
EP3953462A1 (en) | 2019-04-10 | 2022-02-16 | Novozymes A/S | Polypeptide variants |
US20220186151A1 (en) | 2019-04-12 | 2022-06-16 | Novozymes A/S | Stabilized glycoside hydrolase variants |
WO2020229480A1 (en) | 2019-05-14 | 2020-11-19 | Basf Se | Compounds stabilizing hydrolases in liquids |
EP3969556A1 (en) | 2019-05-16 | 2022-03-23 | Unilever Global Ip Limited | Laundry composition |
EP3744838A1 (en) | 2019-05-29 | 2020-12-02 | Novozymes A/S | Lipolytic polymer particles for esterification and interesterification |
EP3750979A1 (en) | 2019-06-12 | 2020-12-16 | Unilever N.V. | Use of laundry detergent composition |
EP3750978A1 (en) | 2019-06-12 | 2020-12-16 | Unilever N.V. | Laundry detergent composition |
US20220364022A1 (en) | 2019-06-28 | 2022-11-17 | Conopco, Inc., D/B/A Unilever | Detergent composition |
EP3990602A1 (en) | 2019-06-28 | 2022-05-04 | Unilever Global IP Limited | Detergent composition |
US20220372408A1 (en) | 2019-06-28 | 2022-11-24 | Conopco, Inc., D/B/A Unilever | Detergent composition |
WO2020260006A1 (en) | 2019-06-28 | 2020-12-30 | Unilever Plc | Detergent compositions |
US20220372397A1 (en) | 2019-06-28 | 2022-11-24 | Conopco, Inc., D/B/A Unilever | Detergent composition |
CN114008183A (zh) | 2019-06-28 | 2022-02-01 | 联合利华知识产权控股有限公司 | 洗涤剂组合物 |
JP2022538360A (ja) | 2019-07-01 | 2022-09-01 | ビーエーエスエフ ソシエタス・ヨーロピア | 酵素を安定化するためのペプチドアセタール |
EP3994255A1 (en) | 2019-07-02 | 2022-05-11 | Novozymes A/S | Lipase variants and compositions thereof |
US20220403298A1 (en) | 2019-07-12 | 2022-12-22 | Novozymes A/S | Enzymatic emulsions for detergents |
EP4022020A1 (en) | 2019-08-27 | 2022-07-06 | Novozymes A/S | Composition comprising a lipase |
WO2021037895A1 (en) | 2019-08-27 | 2021-03-04 | Novozymes A/S | Detergent composition |
WO2021043764A1 (en) | 2019-09-02 | 2021-03-11 | Unilever Global Ip Limited | Detergent composition |
WO2021053127A1 (en) | 2019-09-19 | 2021-03-25 | Novozymes A/S | Detergent composition |
WO2021064068A1 (en) | 2019-10-03 | 2021-04-08 | Novozymes A/S | Polypeptides comprising at least two carbohydrate binding domains |
AR120142A1 (es) | 2019-10-07 | 2022-02-02 | Unilever Nv | Composición detergente |
CN114585718A (zh) | 2019-10-18 | 2022-06-03 | 巴斯夫欧洲公司 | 储存稳定的包含水解酶的液体 |
US20220403359A1 (en) | 2019-10-24 | 2022-12-22 | Danisco Us Inc | Variant maltopentaose/maltohexaose-forming alpha-amylases |
WO2021105330A1 (en) | 2019-11-29 | 2021-06-03 | Basf Se | Compositions and polymers useful for such compositions |
WO2021115912A1 (en) | 2019-12-09 | 2021-06-17 | Basf Se | Formulations comprising a hydrophobically modified polyethyleneimine and one or more enzymes |
WO2021123307A2 (en) | 2019-12-20 | 2021-06-24 | Novozymes A/S | Polypeptides having proteolytic activity and use thereof |
AU2020405786A1 (en) | 2019-12-20 | 2022-08-11 | Henkel Ag & Co. Kgaa | Cleaning compositions comprising dispersins IX |
AU2020404593A1 (en) | 2019-12-20 | 2022-08-18 | Henkel Ag & Co. Kgaa | Cleaning compositions comprising dispersins VI |
EP4077621A2 (en) | 2019-12-20 | 2022-10-26 | Henkel AG & Co. KGaA | Cleaning compositions comprising dispersins viii |
EP4077619A1 (en) | 2019-12-20 | 2022-10-26 | Henkel AG & Co. KGaA | Cleaning composition coprising a dispersin and a carbohydrase |
US20220411726A1 (en) | 2019-12-20 | 2022-12-29 | Novozymes A/S | Stabilized liquid boron-free enzyme compositions |
WO2021130167A1 (en) | 2019-12-23 | 2021-07-01 | Novozymes A/S | Enzyme compositions and uses thereof |
JP2023507760A (ja) | 2019-12-23 | 2023-02-27 | ザ プロクター アンド ギャンブル カンパニー | 酵素を含む組成物 |
WO2021148364A1 (en) | 2020-01-23 | 2021-07-29 | Novozymes A/S | Enzyme compositions and uses thereof |
CN115052981A (zh) | 2020-01-31 | 2022-09-13 | 诺维信公司 | 甘露聚糖酶变体以及编码它们的多核苷酸 |
EP4097226A1 (en) | 2020-01-31 | 2022-12-07 | Novozymes A/S | Mannanase variants and polynucleotides encoding same |
EP3892708A1 (en) | 2020-04-06 | 2021-10-13 | Henkel AG & Co. KGaA | Cleaning compositions comprising dispersin variants |
US20230143128A1 (en) | 2020-04-08 | 2023-05-11 | Novozymes A/S | Carbohydrate binding module variants |
CN115516071A (zh) | 2020-04-21 | 2022-12-23 | 诺维信公司 | 包含具有果聚糖降解活性的多肽的清洁组合物 |
EP3907271A1 (en) | 2020-05-07 | 2021-11-10 | Novozymes A/S | Cleaning composition, use and method of cleaning |
US20230212548A1 (en) | 2020-05-26 | 2023-07-06 | Novozymes A/S | Subtilase variants and compositions comprising same |
EP4162018B1 (en) | 2020-06-08 | 2024-01-31 | Unilever IP Holdings B.V. | Method of improving protease activity |
EP4168523A1 (en) | 2020-06-18 | 2023-04-26 | Basf Se | Compositions and their use |
EP4172298A1 (en) | 2020-06-24 | 2023-05-03 | Novozymes A/S | Use of cellulases for removing dust mite from textile |
EP3936593A1 (en) | 2020-07-08 | 2022-01-12 | Henkel AG & Co. KGaA | Cleaning compositions and uses thereof |
US20230137116A1 (en) | 2020-07-09 | 2023-05-04 | Basf Se | Compositions and their applications |
WO2022008732A1 (en) | 2020-07-10 | 2022-01-13 | Basf Se | Enhancing the activity of antimicrobial preservatives |
CN116057158A (zh) | 2020-07-27 | 2023-05-02 | 联合利华知识产权控股有限公司 | 酶和表面活性剂用于抑制微生物的用途 |
CN116323889A (zh) | 2020-08-25 | 2023-06-23 | 诺维信公司 | 家族44木葡聚糖酶变体 |
EP4204547A1 (en) | 2020-08-28 | 2023-07-05 | Novozymes A/S | Protease variants with improved solubility |
WO2022043042A1 (en) | 2020-08-28 | 2022-03-03 | Unilever Ip Holdings B.V. | Detergent composition |
US20230303951A1 (en) | 2020-08-28 | 2023-09-28 | Conopco, Inc., D/B/A Unilever | Detergent composition |
BR112023003008A2 (pt) | 2020-08-28 | 2023-04-04 | Unilever Ip Holdings B V | Tensoativo de sulfonato de alcano secundário (sas), composição detergente e método de tratamento de um artigo têxtil |
EP4204530A1 (en) | 2020-08-28 | 2023-07-05 | Unilever IP Holdings B.V. | Detergent composition |
CN116096703A (zh) | 2020-08-28 | 2023-05-09 | 联合利华知识产权控股有限公司 | 表面活性剂和洗涤剂组合物 |
JP2023544111A (ja) | 2020-09-22 | 2023-10-20 | ビーエーエスエフ ソシエタス・ヨーロピア | 第二の酵素を含む、プロテアーゼとプロテアーゼ阻害薬との改善された組合せ |
CN116507725A (zh) | 2020-10-07 | 2023-07-28 | 诺维信公司 | α-淀粉酶变体 |
WO2022084303A2 (en) | 2020-10-20 | 2022-04-28 | Novozymes A/S | Use of polypeptides having dnase activity |
WO2022083949A1 (en) | 2020-10-20 | 2022-04-28 | Basf Se | Compositions and their use |
CN116615523A (zh) | 2020-10-28 | 2023-08-18 | 诺维信公司 | 脂氧合酶的用途 |
BR112023008326A2 (pt) | 2020-10-29 | 2023-12-12 | Novozymes As | Variantes de lipase e composições compreendendo tais variantes de lipase |
WO2022103725A1 (en) | 2020-11-13 | 2022-05-19 | Novozymes A/S | Detergent composition comprising a lipase |
WO2022106400A1 (en) | 2020-11-18 | 2022-05-27 | Novozymes A/S | Combination of immunochemically different proteases |
WO2022106404A1 (en) | 2020-11-18 | 2022-05-27 | Novozymes A/S | Combination of proteases |
EP4263773A1 (en) | 2020-12-17 | 2023-10-25 | Unilever IP Holdings B.V. | Cleaning composition |
EP4263771A1 (en) | 2020-12-17 | 2023-10-25 | Unilever IP Holdings B.V. | Use and cleaning composition |
EP4032966A1 (en) | 2021-01-22 | 2022-07-27 | Novozymes A/S | Liquid enzyme composition with sulfite scavenger |
EP4284905A1 (en) | 2021-01-28 | 2023-12-06 | Novozymes A/S | Lipase with low malodor generation |
EP4039806A1 (en) | 2021-02-04 | 2022-08-10 | Henkel AG & Co. KGaA | Detergent composition comprising xanthan lyase and endoglucanase variants with im-proved stability |
WO2022171872A1 (en) | 2021-02-12 | 2022-08-18 | Novozymes A/S | Stabilized biological detergents |
WO2022171780A2 (en) | 2021-02-12 | 2022-08-18 | Novozymes A/S | Alpha-amylase variants |
EP4047088A1 (en) | 2021-02-22 | 2022-08-24 | Basf Se | Amylase variants |
WO2022175435A1 (en) | 2021-02-22 | 2022-08-25 | Basf Se | Amylase variants |
WO2022189521A1 (en) | 2021-03-12 | 2022-09-15 | Novozymes A/S | Polypeptide variants |
WO2022194673A1 (en) | 2021-03-15 | 2022-09-22 | Novozymes A/S | Dnase variants |
EP4060036A1 (en) | 2021-03-15 | 2022-09-21 | Novozymes A/S | Polypeptide variants |
WO2022199418A1 (en) | 2021-03-26 | 2022-09-29 | Novozymes A/S | Detergent composition with reduced polymer content |
WO2022233897A1 (en) | 2021-05-04 | 2022-11-10 | Novozymes A/S | Enzymatic treatment of feedstock for hydrotreated vegetable oil (hvo) production |
EP4359518A1 (en) | 2021-06-23 | 2024-05-01 | Novozymes A/S | Alpha-amylase polypeptides |
EP4134423A1 (en) | 2021-08-12 | 2023-02-15 | Henkel AG & Co. KGaA | Sprayable laundry pre-treatment composition |
CN117957300A (zh) | 2021-09-20 | 2024-04-30 | 联合利华知识产权控股有限公司 | 洗涤剂组合物 |
WO2023061928A1 (en) | 2021-10-12 | 2023-04-20 | Novozymes A/S | Endoglucanase with improved stability |
WO2023061827A1 (en) | 2021-10-13 | 2023-04-20 | Basf Se | Compositions comprising polymers, polymers, and their use |
WO2023088777A1 (en) | 2021-11-22 | 2023-05-25 | Basf Se | Compositions comprising polymers, polymers, and their use |
WO2023114988A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Variant maltopentaose/maltohexaose-forming alpha-amylases |
WO2023116569A1 (en) | 2021-12-21 | 2023-06-29 | Novozymes A/S | Composition comprising a lipase and a booster |
WO2023117989A1 (en) | 2021-12-21 | 2023-06-29 | Basf Se | Environmental attributes for formulation additives |
EP4206309A1 (en) | 2021-12-30 | 2023-07-05 | Novozymes A/S | Protein particles with improved whiteness |
WO2023148086A1 (en) | 2022-02-04 | 2023-08-10 | Basf Se | Compositions comprising polymers, polymers, and their use |
EP4234664A1 (en) | 2022-02-24 | 2023-08-30 | Evonik Operations GmbH | Composition comprising glucolipids and enzymes |
WO2023165507A1 (en) | 2022-03-02 | 2023-09-07 | Novozymes A/S | Use of xyloglucanase for improvement of sustainability of detergents |
WO2023165950A1 (en) | 2022-03-04 | 2023-09-07 | Novozymes A/S | Dnase variants and compositions |
WO2023194204A1 (en) | 2022-04-08 | 2023-10-12 | Novozymes A/S | Hexosaminidase variants and compositions |
WO2023203080A1 (en) | 2022-04-20 | 2023-10-26 | Novozymes A/S | Process for producing free fatty acids |
WO2023227332A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Laundry liquid composition comprising a surfactant, an alkoxylated zwitterionic polyamine polymer and a protease |
WO2023227331A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Composition comprising a specific methyl ester ethoxylate surfactant and a lipase |
WO2023227356A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Composition containing enzyme |
WO2023227375A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Laundry liquid composition comprising a surfactant, an aminocarboxylate, an organic acid and a fragrance |
WO2023227335A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Liquid composition comprising linear alkyl benzene sulphonate, methyl ester ethoxylate and alkoxylated zwitterionic polyamine polymer |
WO2023227421A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Laundry liquid composition comprising a surfactant, an alkoxylated zwitterionic polyamine polymer, and a fragrance |
DE102022205593A1 (de) | 2022-06-01 | 2023-12-07 | Henkel Ag & Co. Kgaa | Wasch- und reinigungsmittel mit verbesserter enzymstabilität |
DE102022205588A1 (de) | 2022-06-01 | 2023-12-07 | Henkel Ag & Co. Kgaa | Wasch- und reinigungsmittel mit verbesserter enzymstabilität |
DE102022205594A1 (de) | 2022-06-01 | 2023-12-07 | Henkel Ag & Co. Kgaa | Leistungsverbesserte und lagerstabile protease-varianten |
DE102022205591A1 (de) | 2022-06-01 | 2023-12-07 | Henkel Ag & Co. Kgaa | Wasch- und reinigungsmittel mit verbesserter enzymstabilität |
WO2023247348A1 (en) | 2022-06-21 | 2023-12-28 | Novozymes A/S | Mannanase variants and polynucleotides encoding same |
WO2023247664A2 (en) | 2022-06-24 | 2023-12-28 | Novozymes A/S | Lipase variants and compositions comprising such lipase variants |
WO2024033136A1 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Amylase variants |
WO2024033134A1 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Enzyme compositions comprising protease, mannanase, and/or cellulase |
WO2024033135A2 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Amylase variants |
WO2024033133A2 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Enzyme compositions comprising an amylase |
EP4324900A1 (en) | 2022-08-17 | 2024-02-21 | Henkel AG & Co. KGaA | Detergent composition comprising enzymes |
WO2024056333A1 (en) | 2022-09-13 | 2024-03-21 | Unilever Ip Holdings B.V. | Washing machine and washing method |
WO2024056331A1 (en) | 2022-09-13 | 2024-03-21 | Unilever Ip Holdings B.V. | Washing machine and washing method |
WO2024056334A1 (en) | 2022-09-13 | 2024-03-21 | Unilever Ip Holdings B.V. | Washing machine and washing method |
WO2024056278A1 (en) | 2022-09-13 | 2024-03-21 | Unilever Ip Holdings B.V. | Washing machine and washing method |
EP4349946A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Unit dose fabric treatment product |
EP4349942A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Laundry liquid composition |
EP4349947A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Laundry liquid composition |
EP4349948A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Laundry liquid composition |
EP4349945A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Laundry liquid composition |
EP4349943A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Laundry liquid composition |
EP4349944A1 (en) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Laundry liquid composition |
WO2024079301A1 (en) | 2022-10-14 | 2024-04-18 | Novozymes A/S | Process for selective hydrolysis of diglycerides in an oil/fat with aid of candida antarctica lipase b |
WO2024083589A1 (en) | 2022-10-18 | 2024-04-25 | Basf Se | Detergent compositions, polymers and methods of manufacturing the same |
WO2024083819A1 (en) | 2022-10-20 | 2024-04-25 | Novozymes A/S | Lipid removal in detergents |
WO2024088716A1 (en) | 2022-10-25 | 2024-05-02 | Unilever Ip Holdings B.V. | Composition |
WO2024088706A1 (en) | 2022-10-25 | 2024-05-02 | Unilever Ip Holdings B.V. | Composition |
EP4361239A1 (en) | 2022-10-25 | 2024-05-01 | Unilever IP Holdings B.V. | Laundry liquid composition |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1987000859A1 (en) * | 1985-08-09 | 1987-02-12 | Gist-Brocades N.V. | Novel lipolytic enzymes and their use in detergent compositions |
JPH0755148B2 (ja) * | 1986-03-12 | 1995-06-14 | 生体機能利用化学品新製造技術研究組合 | リパ−ゼの製造法 |
JP3029435B2 (ja) * | 1988-03-25 | 2000-04-04 | ジェネンコー インターナショナル インコーポレイテッド | 脂質分解性酵素をコードする遺伝子の分子クローニング及び発現 |
US5063160A (en) * | 1989-03-16 | 1991-11-05 | Olin Corporation | Identification, characterization, and method of production of a novel microbial lipase |
US5059341A (en) * | 1989-03-16 | 1991-10-22 | Olin Corporation | Cleaning composition comprising microbial lipase SD2, sodium dodecylbenzene sulfonate and gelatin |
US5227300A (en) * | 1989-03-16 | 1993-07-13 | Olin Corporation | Identification, characterization and method of production of a novel microbial lipase |
EP0486073B1 (en) * | 1990-11-14 | 1996-07-24 | The Procter & Gamble Company | Liquid detergent composition containing lipase and protease |
AU4231493A (en) * | 1992-05-08 | 1993-12-13 | Procter & Gamble Company, The | Granular detergent compositions with lipase |
JP3072579B2 (ja) * | 1992-05-27 | 2000-07-31 | ノボ ノルディスク アクティーゼルスカブ | アルカリリパーゼ、それを生産する微生物およびアルカリリパーゼ含有洗剤組成物 |
US5454971A (en) * | 1992-05-27 | 1995-10-03 | Showa Denko K.K. | Alkaline lipase, method for producing the same, microorganism producing the same and detergent composition containing alkaline lipase |
DE4411349A1 (de) * | 1994-03-31 | 1995-10-05 | Henkel Kgaa | Lipasehaltiges Textilwaschmittel |
-
1993
- 1993-08-30 JP JP5214506A patent/JP2859520B2/ja not_active Expired - Fee Related
-
1994
- 1994-08-26 WO PCT/JP1994/001416 patent/WO1995006720A1/ja not_active Application Discontinuation
- 1994-08-26 AU AU75087/94A patent/AU7508794A/en not_active Abandoned
- 1994-08-26 US US08/605,015 patent/US5827718A/en not_active Expired - Fee Related
- 1994-08-26 CN CN94193220A patent/CN1072718C/zh not_active Expired - Fee Related
- 1994-08-26 EP EP94925013A patent/EP0721981A4/en not_active Withdrawn
-
1996
- 1996-02-29 FI FI960949A patent/FI960949A0/fi not_active IP Right Cessation
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100419126C (zh) * | 2005-06-16 | 2008-09-17 | 广州天至环保科技有限公司 | 一种应用于金属加工业前处理除油脱脂的生物除油剂及其处理金属表面油污的方法 |
CN102226173A (zh) * | 2011-05-06 | 2011-10-26 | 华南理工大学 | 一种稳定型酶制剂及其制备方法和应用 |
CN102226173B (zh) * | 2011-05-06 | 2014-02-12 | 华南理工大学 | 一种稳定型酶制剂及其制备方法和应用 |
CN103451013A (zh) * | 2013-09-25 | 2013-12-18 | 陕西合盛生物柴油技术开发有限公司 | 废弃油脂的预处理方法 |
CN107488615A (zh) * | 2017-09-18 | 2017-12-19 | 山东隆科特酶制剂有限公司 | 一株高产脂肪酶的假单胞菌及其发酵产酶方法 |
CN107488615B (zh) * | 2017-09-18 | 2020-01-14 | 山东隆科特酶制剂有限公司 | 一株高产脂肪酶的假单胞菌及其发酵产酶方法 |
Also Published As
Publication number | Publication date |
---|---|
JP2859520B2 (ja) | 1999-02-17 |
JPH0767636A (ja) | 1995-03-14 |
EP0721981A1 (en) | 1996-07-17 |
FI960949A (fi) | 1996-02-29 |
AU7508794A (en) | 1995-03-22 |
CN1072718C (zh) | 2001-10-10 |
FI960949A0 (fi) | 1996-02-29 |
EP0721981A4 (en) | 2002-07-31 |
WO1995006720A1 (fr) | 1995-03-09 |
US5827718A (en) | 1998-10-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1072718C (zh) | 脂肪酶及其微生物、该脂肪酶的制造方法及用途 | |
EP0214761B1 (en) | An enzymatic detergent additive, a detergent, and a washing method | |
CA1250537A (en) | Enzymatic detergent additive, a detergent, and a washing method | |
EP0218272B1 (en) | Novel lipolytic enzymes and their use in detergent compositions | |
CN1062906C (zh) | 液化碱性α-淀粉酶、其制备方法及含有它的洗涤剂组合物 | |
EP0258068B1 (en) | Enzymatic detergent additive | |
DE69731195T2 (de) | Alkalisches, lipolytisches enzym | |
EP0571982A1 (en) | Alkaline lipase, method for producing the same, microorganism producing the same and detergent composition containing alkaline lipase | |
US20040053360A1 (en) | Lipase variants | |
JPH0343073A (ja) | 種々の温度で活性を有する特異な微生物リパーゼ及び該リパーゼを含有する洗剤 | |
DE69633676T2 (de) | Alkaline protease, verfahren zu ihrer herstellung, anwendung derselben und diese herstellende mikroorganismen | |
DE10328887B4 (de) | Alkalische Protease | |
CN1092101A (zh) | 织物洗涤和柔软用组合物中使用的纤维素酶颗粒 | |
US6306813B1 (en) | Alkaline lipase and detergent composition active at low temperature | |
JP3072579B2 (ja) | アルカリリパーゼ、それを生産する微生物およびアルカリリパーゼ含有洗剤組成物 | |
CN86106566A (zh) | 新的脂解酶及其在除垢组合物中的应用 | |
BE1009844A3 (fr) | Procede de recolte d'une proteine. | |
JPS63132998A (ja) | 洗浄剤組成物 | |
JP3823310B2 (ja) | 新規アルカリリパーゼおよびその製造法 | |
BE1008783A3 (fr) | Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci. | |
JPH08283787A (ja) | 漂白剤存在下の洗剤溶液中で高活性を有するリパーゼを含む洗剤組成物 | |
JP2588009B2 (ja) | 新規リパーゼaks及び該リパーゼを有効成分として含有する洗浄剤組成物 | |
CN1177979A (zh) | 新脂酶和其产生方法以及产生这种脂酶的微生物 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C53 | Correction of patent for invention or patent application | ||
CB02 | Change of applicant information |
Address after: Danish bagues waddan Applicant after: Novo Nordisk A/S Address before: Tokyo port area Applicant before: Showa Denko K. K. |
|
COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: SHOWA DENKO K. K. TO: NOVO NORDISK A / S |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C19 | Lapse of patent right due to non-payment of the annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |