CN105939767A - 具有双声电泳腔的声电泳装置 - Google Patents

具有双声电泳腔的声电泳装置 Download PDF

Info

Publication number
CN105939767A
CN105939767A CN201580004104.2A CN201580004104A CN105939767A CN 105939767 A CN105939767 A CN 105939767A CN 201580004104 A CN201580004104 A CN 201580004104A CN 105939767 A CN105939767 A CN 105939767A
Authority
CN
China
Prior art keywords
sound
flow
piezoelectric element
operatic tunes
electrophoretic apparatus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201580004104.2A
Other languages
English (en)
Other versions
CN105939767B (zh
Inventor
巴特·利普肯斯
布莱恩·麦卡西
本·罗斯-约翰斯路德
杰森·巴恩斯
戴恩·米利
托马斯·J·肯尼迪三世
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Flodesign Sonics Inc
Original Assignee
Flodesign Sonics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Flodesign Sonics Inc filed Critical Flodesign Sonics Inc
Publication of CN105939767A publication Critical patent/CN105939767A/zh
Application granted granted Critical
Publication of CN105939767B publication Critical patent/CN105939767B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/28—Mechanical auxiliary equipment for acceleration of sedimentation, e.g. by vibrators or the like
    • B01D21/283—Settling tanks provided with vibrators

Landscapes

  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)

Abstract

声电泳装置包括具有压电元件(160)的声腔(155，157)，该压电元件位于声腔的容积中。压电元件振动并从两侧产生声驻波，使得可以从穿过声腔的流体中分离出颗粒。这允许更有效地冷却压电元件，从而减少行进通过装置的流体中的瞬态热载荷。

Description

具有双声电泳腔的声电泳装置
相关申请的交叉引用
本申请要求2014年1月8日提交的美国临时专利申请No.61/925,171的优先权。该申请的全部内容在此通过引用完全并入本文。
背景技术
在许多应用中需要将颗粒/流体混合物分离成它的多个单独组分的能力。声电泳(acoustophoresis)使用高强度声波分离颗粒，而不需要使用膜或物理的尺寸排阻过滤器。已知的是，当强度和压缩系数这两者中存在差异(也被称为对比系数)时，声音的高强度驻波可以对流体中的颗粒施加力。驻波具有表现出随时间“驻立”不动的压力分布。驻波中的压力分布在其波节和波腹处包含净零压力的区域。取决于颗粒的密度和压缩系数，将在驻波的波节或波腹处捕获这些颗粒。驻波的频率越高，能够被捕获的颗粒越小。
常规声电泳装置因如下几个因素而具有有限的功效，这些因素包括发热、对流体流动的限制以及不能捕获不同类型的材料。具体而言，发热可能对流体流中的材料有害，特别是在如下生物制药应用中：在流体流中存在例如中国仓鼠卵巢(CHO)细胞和蛋白质等材料以及从这些材料提取的单克隆抗体。
就这一点而言，包括压电元件的超声换能器通常用于产生超声波。换能器通常被安装到腔的壁部中，而反射体被安装在相对的壁部中。反射体的表面与压电元件的表面平行，从而使压电元件所产生的入射波的反射最大化，以形成驻波。当进行声电泳时，在操作期间压电元件产生热量。期望提供能够最大程度地减少发热的替代设计。
发明内容
本发明涉及多种声电泳系统，其包括位于流动路径中间而不是位于流动路径一侧的压电元件。这允许压电元件两侧而不仅是压电元件一侧产生声驻波。这还允许压电元件两侧暴露于流体流并产生冷却效果，从而减轻压电元件中的热积聚。该装置可以用于使颗粒与颗粒/流体混合物分离。可以得到颗粒浓度增大的新混合物，或者可以得到分离出的颗粒本身。在更多特定实施例中，颗粒是生物细胞，例如中国仓鼠卵巢(CHO)细胞、NS0杂交瘤细胞、幼仑鼠肾(BHK)细胞和人体细胞。本文描述了几种不同类型的组件和整体系统。
本文多个实施例公开了一种声电泳装置，包括：装置入口，其允许流体流进入到装置中；装置出口，其允许流体从该装置流出；以及声腔，其位于装置入口与装置出口之间的流动路径中。声腔包括：第一端以及与第一端相对的第二端；压电元件，其在第一端与第二端之间延伸，并将声腔分成第一流动腔和第二流动腔，压电元件具有第一表面和第二表面；第一反射体，其与压电元件的第一表面相对，第一反射体与第一表面之间设置有第一流动腔；以及第二反射体，其与压电元件的第二表面相对，第二反射体与第二表面之间设置有第二流动腔。
声腔还可以包括：保持板，其保持压电元件；以及两个支架板，其具有狭槽，该狭槽用于将保持板保持在声腔中的固定位置。
压电元件可以包括多个压电晶体。一般来说，压电元件适合于在第一流动腔和第二流动腔中产生多维驻波。在更多特定实施例中，在第一流动腔和第二流动腔中由压电元件产生不同的驻波(例如，其具有不同的频率)。
在一些实施例中，声电泳装置成形为：使得流体经由装置入口流动到装置中，进入到声腔的第一端中，然后并行地流动穿过第一流动腔和第二流动腔，经由声腔的第二端从声腔流出，并经由装置出口从声电泳装置流出。声电泳装置还包括波状喷嘴壁，该波状喷嘴壁位于装置入口与声腔之间。
在其他实施例中，声电泳装置成形为：使得流体经由装置入口流动到装置中，然后沿从声腔的第一端经由第一流动腔至第二端接着经由第二流动腔返回至第一端的U形路径行进通过声腔，然后经由声腔的第一端离开流动腔，并经由装置出口离开装置。在此类实施例中，声腔的第二端可以通向井部，井部的横截面面积从单个入口向下渐缩至顶点，并且排出管线将顶点连接至用于回收在井部中收集的材料的端口。
本文还公开了使颗粒与主流体(host fluid)分离的方法，该方法包括：使主流体和颗粒的混合物流动穿过如上所述的声电泳装置，该声电泳装置具有第一流动腔和第二流动腔。脉冲电压信号驱动压电元件，以在第一流动腔和第二流动腔中产生多维驻波，从而使颗粒与主流体分离。
在第一流动腔和第二流动腔这两者中，多维驻波可以产生具有轴向力分量和横向力分量的声辐射力，轴向力分量和横向力分量具有相同的数量级。
在特定实施例中，颗粒是中国仓鼠卵巢(CHO)细胞、NS0杂交瘤细胞、幼仑鼠肾(BHK)细胞或人体细胞。
脉冲电压信号可以具有正弦、方形、锯齿或三角形波形。脉冲电压信号可以具有500kHz至10MHz的频率。可以利用幅度或频率调制开启/停止能力驱动脉冲电压信号，以消除声流。
在特定实施例中，主流体和颗粒的混合物在进入所述声腔之前具有1500以下的雷诺数。有时，混合物向上竖直流动，而颗粒向下下沉至收集导管。
本文多个实施例还公开了一种声电泳装置，包括：装置入口，其允许流体流进入到装置中；装置出口，其允许流体从装置流出；以及声腔，其位于装置入口与装置出口之间的流动路径中。声腔包括：多个压电元件，其在第一端与第二端之间延伸；以及多个反射体。每个压电元件均具有第一表面和第二表面；并且每个压电元件均位于两个反射体之间。
在下文更具体地描述了这些和其他非限制性特征。
附图说明
以下是附图的简单描述，这些附图是为了说明本发明所公开的示例性实施例而提供的，而不为了限制本发明。
图1是包括本发明的“双声电泳腔”的声电泳装置的外部透视图。在该视图中，用于给压电元件供电的连接器面板是可见的。
图2是仅双声电泳腔的透视图，且连接器面板位于附图的顶部。
图3是保持板的透视图，该保持板保持位于两个支架板之间的压电元件，这两个支架板将保持板保持在声腔中的适当位置。
图4是示出了保持板和支架板的图片。压电元件由两个分离的矩形压电晶体组成。
图5是更详细地示出了保持板和压电元件的图片。
图6是第二示例性实施例的透视图。这里，两个压电元件被放置在声腔容积中。在声腔中总共存在三个反射体，两个反射体位于相对的壁部上，而另一个反射体位于声腔的中部。声腔包含四个不同的流动路径，流体/颗粒混合物可以流动穿过这些流动路径。
图7示出了与本发明的双声电泳腔一起使用的示例性声电泳分离器。这里，流体从分离器顶部流动穿过该腔并从分离器的底部流出。
图8是在图7的分离器中的波状喷嘴壁与收集导管的相交处附近的流体流的放大视图。
图9是第二示例性声电泳装置的正视图，其中，流体经由装置顶部处的入口流入，并随后沿U形流动路径行进通过声电泳腔，然后同样经由装置顶部处的出口离开。利用装置底部处的井部收集分离出的颗粒。
图10是示出了声辐射力、浮力和斯托克斯(Stokes)曳力与粒度的关系的曲线图。横轴以微米(μm)为单位，而纵轴以牛顿(N)为单位。
图11是示出了正在运行的本发明的双声电泳腔的图片。捕获线是可见的。
图12是示出了对常规系统和本发明的双声电泳腔这两者而言随着时间的装置效率以及随着时间的等效操作的曲线图。
具体实施方式
通过参考优选实施例及其所包含的实例的以下具体描述，可以更容易地理解本发明。在以下说明书以及所附权利要求中，将参考限定为具有下述含义的大量术语。
虽然为了清楚起见在以下描述中使用了特定术语，但这些术语仅意图指代图中被选择用于说明的实施例的特定结构，而不意图限定或限制本发明的范围。在附图和以下描述中，应当理解的是，相同的数字标号指代相同功能的部件。此外，应当理解的是，附图未按比例绘制。
除非上下文另有明确说明，否则单数形式“一个”、“一种”和“该”也包括复数对象。
如在说明书中及权利要求中所使用的那样，术语“包含”可以包括“由…组成”和“基本上由…组成”的实施例。如本文所使用的那样，术语“包含”、“包括”、“具有”、“有”、“可以”、“含有”及其变体意图作为开放式过渡词、术语或单词，这些词语需要存在指定的组分/步骤且允许存在其他组分/步骤。然而，这种描述还应解释为将“由…组成”和“基本上由…组成”的合成物或过程描述成列举的组分/步骤，这允许仅存在指定的组分/步骤且伴随有可能因这些组分而产生的任何杂质，但不包括其他组分/步骤。
数值应当理解为包括：在减少到相同数量的有效数字时是相同的数值；以及按照比本申请中所述类型的常规测量技术的实验误差小的方式偏离设定值以确定该值的数值。
本文所公开的所有范围都包括所述端值，并且可以独立组合(例如，“从2克至10克”的范围包括端值2克和10克以及所有中间值)。
术语“约”可以用于包括能够在不改变该值的基本功能的情况下变化的任意数值。当在范围内使用时，术语“约”也公开了由两个端值的绝对值限定的范围，例如，“约2至约4”也公开了“从2至4”的范围。术语“约”可以指代指示数字的+/-10％。
应注意的是，本文所使用的许多术语是相对术语。例如，术语“上”和“下”在位置上是彼此相对而言的，即，在给定取向上，上组件位于比下组件更高的海拔高度，但如果使装置倒转，则这些术语可能变化。术语“入口”和“出口”是对于给定结构而言相对于流动穿过它们的流体来说的，例如，流体经由入口流动到该结构中，并且经由出口流动到该结构外面。术语“上游”和“下游”是相对于流体流动穿过不同组件的方向而言的，即，流体在流动穿过下游组件之前流动穿过上游组件。应注意的是，在环路中，第一组件既可以描述为第二组件的上游，也可以描述为第二组件的下游。
术语“水平”和“竖直”用于表示相对于绝对基准(即，地平面)的方向。然而，这些用语不应当解释为要求这些结构彼此绝对平行或绝对垂直。例如，第一竖直结构和第二竖直结构不必彼此平行。术语“向上”和“向下”也是相对于绝对基准而言；向上流动总是克服地球的重力。
本申请涉及“相同的数量级”。如果较大数除以较小数得到的商是小于10的值，则这两个数具有相同的数量级。
本发明的声电泳分离技术采用超声驻波捕获(即，保持为固定)主流体流中的第二相粒子(secondary phase particle)。这是与以前方法的重要区别，在以前方法中，颗粒运动轨迹仅由声辐射力的作用改变。声场对颗粒的分散会产生三维声辐射力，作为三维捕获场起作用。当颗粒相对于波长较小时，声辐射力与颗粒体积(例如，半径的立方)成正比。声辐射力与频率和声学对比系数(acoustic contrast factor)成正比。声辐射力还与声能(例如，声压幅值的平方)成比例。对于谐波激励而言，力的正弦空间变化将颗粒驱动到驻波中的稳定位置。当施加在颗粒上的声辐射力强于流体曳力与浮力、重力的组合作用时，颗粒被捕获在声驻波场中。这导致被捕获的颗粒的集中、聚集和/或聚结。此外，诸如皮叶克尼斯(Bjerkness)力等二次(secondary)颗粒间作用力有助于颗粒聚集。使重于主流体(即，密度大于主流体)的颗粒借助于增强的重力沉降而分离。
声电泳装置的一个具体应用是处理生物反应器材料。重要的是能够从流体流中的压出(expressed)材料过滤出细胞和细胞碎片。压出材料由例如重组蛋白或单克隆抗体等生物分子构成，并且是要回收的所需产物。通过使用声电泳，对细胞和细胞碎片的分离是非常有效的，并且导致非常低的压出材料损耗。这是对现有过滤处理(深层过滤、切向流动过滤、离心分离)的改进，现有过滤处理在高细胞密度的情况下表现出有限的效率，使得滤床本身中的压出材料的损耗可能高达生物反应器所产生的材料的5％。包括中国仓鼠卵巢(CHO)、NS0杂交瘤细胞、幼仓鼠肾(BHK)细胞和人体细胞在内的哺乳动物细胞培养物的使用已被证明是生产/压出当今制药所需的重组蛋白和单克隆抗体的非常有效的方法。借助于声电泳对哺乳动物细胞和哺乳动物细胞碎片的过滤有助于大大提高生物反应器的产量。
就这一点而言，对比系数是颗粒和流体本身的压缩系数与密度之间的差异。这些特性是颗粒和流体本身的特征。与处于悬浮的介质相比，大多数细胞种类表现出更高的密度但更低的压缩系数，使得细胞与介质之间的声学对比系数具有正值。结果，轴向声辐射力(ARF)将具有正对比系数的细胞驱动到压力波节面，而将具有负对比系数的细胞或其他颗粒驱动到压力波腹面。声辐射力的径向或横向分量帮助捕获细胞。ARF的径向或横向分量比流体曳力和重力的组合作用大。
随着细胞聚集在驻波的波节处，还存在细胞培养介质的物理洗涤(scrubbing)，当出现该物理洗涤时，由于细胞会与已保持在驻波中的细胞接触，因此更多的细胞被捕获。这通常使细胞与细胞培养介质分离。压出的生物分子保留在营养流体流(即，细胞培养介质)中。
期望的是，超声换能器在流体中产生三维或多维超声驻波，该三维或多维超声驻波对悬浮颗粒施加与轴向力共存的横向力，从而增强驻波的颗粒捕获能力。发表在文献中的典型结果论述到：横向力比轴向力小一个数量级。与之相反，本申请中所公开的技术提供了具有与轴向力相同的数量级的横向力。
也可以用任意定相来驱动多个超声换能器。换言之，多个换能器可以在彼此异相的同时工作，以分离流体流中的材料。作为选择，已被划分成有序阵列的单个超声换能器也可以被操作，使得该阵列的某些部分与阵列的其它部分异相。
由一个或多个压电式换能器产生三维(3-D)或多维声驻波，换能器是电激励或机械激励的，使得三维或多维声驻波以多激励模式移动。由此产生的波的类型可以被表征为复合波，该复合波具有与泄漏的对称(也称为压缩或延伸)蓝姆波相似的位移轮廓。因为波辐射到水层中，所以波会泄漏，这导致在水层中产生声驻波。对称蓝姆波具有相对于压电元件的中性轴对称的位移轮廓，这使得在三维空间中产生多个驻波。利用波产生的这种方式，如果压电式换能器以“活塞(piston)”模式(仅产生单个平面驻波)被激励，则产生更大的横向捕获力。因此，在相同输入功率输入到压电式换能器的情况下，三维或多维超声驻波可以具有较高的横向捕获力，该横向捕获力可以比活塞模式中所产生的单个超声驻波强10倍或10倍以上。
有时，由于声流而可能需要调制驻波的频率或电压幅度。这可以借助于幅度调制和/或频率调制来完成。驻波传播的占空比也可以被利用，以实现材料捕获的某些结果。换言之，为了实现所需结果，可以在不同频率下开启或切断声束。
当进行声电泳时，涉及使用压电晶体产生驻波的一些变量是电压输入、压电晶体的品质因数、包含压电晶体的全部换能器的阻抗以及在单元操作期间由压电晶体/换能器产生的温度或热量。如先前讨论的，热量可能对流体中的材料(例如，生物细胞或产物)有害。现有装置已使用各种方法和流体流型来驱散声电泳期间产生的热量，或已调制压电晶体的扰动来减少输入到系统中的热量。
本发明涉及多种声电泳装置，其中，产生驻波的压电元件被放置在声腔容积中，使得流体/颗粒混合物在压电元件两侧流动，并且可以在压电元件两侧产生驻波。这还可以称为“双声电泳腔”。两侧上的流体流的冷却效果能够减少可能在压电元件中发生的热积聚。流体可以是液体(例如，水)或可以是气体(例如，空气)。
图1是包括双声电泳腔的基本声电泳装置100的外部透视图。这里的基本装置被示出为由独立部件形成。这些部件包括入口组件110、连接组件120、声腔130和出口组件105。可以看到声腔的两个外壁。一个外壁上存在反射体144，而另一个外壁上存在连接器面板188，该连接器面板188用于给声腔内部的压电元件(不可见)提供电力。
在图1中，流体流是向上的。简而言之，入口组件包括入口112，该入口112通入到环形增压室(plenum)114中。经由入口泵入流体/颗粒混合物。混合物在压力作用下向上流入到具有波状喷嘴壁122的连接组件120中，该波状喷嘴壁122减小了流体流动路径的横截面。随着流体被持续泵入到流体路径中，最终，入口组件110和声腔130充满流体，并且流体压力上升到足够高，使得流体将经由装置顶部处的出口106流出。超声驻波内的颗粒凝聚或聚集，并最终变大至重力克服了驻波的声学力的尺寸，从而使颗粒聚集，然后经由收集导管116掉落/下沉，该收集导管116被环形增压室114包围。然后，颗粒可以被收集，或者作为更集中的流体/颗粒混合物或简单地作为颗粒本身被收集。
图2是图1的声腔的透视图。声腔130包括具有第一端132和第二端134的壳体131，第一端132和第二端134位于壳体的相反两端。这里，壳体呈如下立方体的形状：该立方体具有四个侧壁151和152(第三侧壁和第四侧壁不可见)、第一壁部136和第二壁部138。然而，组件的外部形状不特别有实质意义，并可以是例如圆柱形。壳体的第一端和第二端可以被视为限定了z轴。四个相对侧壁可以被视为与沿着壳体的x轴、y轴的相反方向对应。
壳体的第一端132与第二端134之间限定有流动通道155。换言之，第一壁部和第二壁部这两者中存在开口，并且孔将这两个开口连接在一起，使得流体可以从第一端与第二端之间流动穿过壳体。如这里所示，孔具有矩形横截面。压电元件位于流动通道的容积中。如这里所示，存在两个反射体144和146，这两个反射体144和146位于压电元件160的相反两侧。两个反射体的表面与压电元件的两个表面平行。每个反射体均为实体或柔性的，并可以由例如钢或钨等高声阻抗材料制成，从而提供良好的反射。壳体的另一侧壁上的连接器面板188也是可见的。连接器可以是例如BNC连接器等任意适合类型的连接器。
图3是声腔的特定部分的透视图。就这一点而言，压电元件160位于声腔的容积中。压电元件160、第一反射体144和第二反射体146的组合将声腔130和流动通道155分成第一流动腔157和第二流动腔159。如这里所示，压电元件160由两个矩形压电晶体162组成。一般来说，任意数量的压电晶体都可以用于组成压电元件，并且这些晶体可以相对于彼此沿任意方向取向。压电晶体被保持板170保持在适当的位置。保持板本身被两个支架板180保持在适当的位置。
压电元件160具有第一表面164和第二表面166。将图2和图3一起研究，第一反射体144与第一表面164相对。第一流动腔157位于第一反射体和第一表面之间。类似地，第二反射体146与第二表面166相对，且第二流动腔159位于第二反射体146与第二表面166之间。需要注意的是，反射体144和146的位置取决于压电元件160的位置；如在下文中将进一步看到的是，反射体不需要设置在壳体的壁部中。然而，一般来说，压电元件的表面与声腔的壁部平行，并且反射体也位于声腔的相对壁部中，该反射体与压电元件的表面平行。
图4是示出了与保持板170分离的支架板(左侧)180的图片。值得注意的是，支架板是实体件；流体不会流动穿过支架板。如这里所示，每个支架板180均包括中央框182以及围绕该中央框182外周的边缘板184，以将支架板连接至壳体。在不同实施例中可以不需要边缘板184，例如，壳体具有仅需将中央框182连接至壳体的不同形状。每个支架板的中央框182还均包括至少一个狭槽186，保持板170的边缘被插入到狭槽186中，以将保持板固定在声腔容积中的固定位置。如下文进一步讨论的那样，也可以将反射体固定到狭槽中。右侧的支架板保持固定板170，并还设置有用于向压电元件提供电力的连接器面板188。
图5是示出了用于压电元件的保持板170的图片。这里，保持板由钢制成，并还设置有用于两个压电晶体162的空间。每个压电晶体的周围均设置有硅灌封层/衬垫172，并将晶体保持在由保持板提供的框架中。还将电引线174与每个晶体的一端焊接在一起。利用该构造，意图使得流体/颗粒混合物不能够从第一流动腔经由保持板流动至第二流动腔。确切的说，流体/颗粒混合物从声腔的一端开始流动并通过一个流动腔，然后从声腔的另一端离开。
值得注意的是，虽然这里保持板和支架板被描述为独立部件，但保持板和支架板也可以被制成为一个一体件。
压电晶体通常由PZT-8(锆钛酸铅)制成。这种晶体可以具有1英寸的长度/宽度和标称2MHz的谐振频率。每个压电元件可以仅由一个晶体形成，或由多个晶体形成，每个晶体均用作独立的超声换能器并受到一个或多个放大器控制。这允许每个晶体以具有高品质因数的其固有模式之一振动。振动晶体直接暴露于流动穿过声腔的流体。
压电晶体上缺少背衬(backing)(例如，使空气作为晶体的背衬)还允许每个压电晶体以具有较小阻尼的高次振动模式(例如，高次模态位移)振动。晶体的振型的幂次越高，晶体就具有越多的节线。虽然捕获线(trapping line)与波节的相关性不必是一对一对应的，并且以较高频率驱动晶体不一定产生更多的捕获线，但晶体的高次模态位移会产生更多的捕获线。
使压电晶体与流体直接接触还因避免了阻尼和能量吸收作用而有助于得到高品质因数。在实施例中，可以涂覆压电晶体以防止含有引线的PZT与主流体接触。例如，以上所述内容在例如分离血液等生物应用中是需要的。这种应用可以使用例如铬、电解镍或化学镀镍等耐磨层。化学气相沉积还可以用于施加聚酯的(对苯二甲)(例如聚对二甲苯)或其他聚合物材料的层。有机物以及例如硅树脂或聚氨酯等生物相容性涂层也可用作耐磨表面。
值得注意的是，取决于电输入如何扰动压电晶体，压电元件的第一表面和第二表面上所产生的驻波可以不同。在特定实施例中，驻波彼此相差至少50千赫(kHz)。
一般来说，本发明涉及使用压电元件在两侧产生驻波，而不是如常规装置所实现的那样仅在一侧产生驻波。本发明设想声腔中可以存在一个以上的这种压电元件。图6是这种实施例的视图。在该实施例中，存在用于压电元件160和165的两个保持板170和175。现在，在该实施例中总共存在三个反射体。与图2相比，反射体144还可以被视为位于两个压电元件之间的中间反射体。反射体146和中间反射体144位于第一压电元件的相反两侧。中间反射体144和第三反射体148位于第二压电元件172的相反两侧。反射体的表面与压电元件的表面平行。中间反射体144反射由压电元件160和165这两者产生的波。
现在存在四个不同的流动腔，流体可以经由这些流动腔流动经过压电元件。反射体146与第一压电元件160之间设置有第一流动腔192。中间反射体144与第一压电元件160之间设置有第二流动腔194。中间反射体144与第二压电元件165之间设置有第三流动腔196。反射体148与第二压电元件165之间设置有第四流动腔198。值得注意的是，在该特定实施例中，支架板180将包括三个狭槽，两个狭槽用于保持板170和175，而另一个狭槽用于中间反射体144。
能够设想的是，可以根据使用者的需要将任意数量(例如，三、四、六、八、十、十二或甚至更多)的压电元件放置在声腔的容积中。对n个压电元件而言，声腔中必须存在(n+1)个反射体，且每个压电元件位于两个反射体之间。此外，压电元件的表面应与每个反射体的表面平行。因此，压电元件和反射体的实际数量受到声腔容积的制约。
图7和图8是一种类型的声电泳装置的侧视图，在该声电泳装置中，可以使用双声电泳腔。这里，沿流动方向为向下的取向示出该装置，该装置用于将低密度颗粒与主流体分离开。然而，该装置本质上可以颠倒，以允许分离出比主流体重的颗粒(与图1类似)。替代沿向上方向的浮力，聚集颗粒的重量利用重力向下拉动聚集颗粒。应注意的是，该实施例描述为具有流体沿竖直方向流动的取向。然而，还可以设想的是，流体流可以在水平方向上或呈一定角度。
含有颗粒的流体经由入口226流动到装置中，从而进入环形增压室231中。环形增压室具有环形内径和环形外径。在本图中可见两个入口，但可以设想的是，可以根据需要设置任何数量的入口。在特定实施例中，使用四个入口。这些入口在径向上相反且沿径向取向。
波状喷嘴壁229以在壁部区域附近产生更高速度并减少紊流的方式减小流动路径的外径，从而在形成流体速度分布时产生邻近的活塞流，即，流体沿中心线的方向向下加速，且几乎没有圆周运动分量并且具有低流动紊流。这将生成最佳用于声学分离和颗粒收集的腔流分布。流体穿过连接导管227并进入声腔228中。
如在图8的放大波状喷嘴229中所看到的，喷嘴壁还对悬浮颗粒增加了径向运动分量，以使颗粒移动成靠近设备的中心线，并与受到上升浮力的凝聚颗粒产生更多的碰撞。该径向运动将允许来自流体中的颗粒在到达分离腔之前在连接导管227中被最佳地洗涤。波状喷嘴壁229以在收集导管233的入口处产生大规模涡流的方式引导流体，从而也增强颗粒收集。一般来说，装置224的流动面积被设计为从环形增压室231至声腔228连续减小，以确保低紊流和涡流的形成，从而更好地进行颗粒分离、聚集和收集。喷嘴壁具有宽端部和窄端部。术语洗涤用于描述：在较大颗粒/液滴沿与流体流相反的方向行进并与较小颗粒碰撞从而有效地从悬浮液中洗涤出较小颗粒时发生的颗粒/液滴聚集、聚合、凝结或结合的过程。
返回参考图7，声腔228包括位于腔的相反两侧的反射体232以及位于腔中的压电元件。在使用中，在压电元件与每个反射体232之间形成驻波234。这些驻波可以用于聚集颗粒，并且该取向用于聚集上浮颗粒(例如，油)。流体/颗粒混合物沿相同方向(即，并行地)流动穿过两个流动腔。含有残留颗粒的流体随后经由出口235从装置离开或流出。
随着上浮颗粒聚集，这些悬浮颗粒最终克服流体流动曳力和声学辐射力的组合作用，并且悬浮颗粒的浮力236足以使上浮颗粒向上上升。就这一点而言，收集导管233被环形增压室231包围。较大颗粒将穿过该导管并进入到收集腔240中。该收集腔还可以作为出口导管的一部分。收集导管和流动出口位于装置的相反两端上。
应注意的是，形成在分离腔228中的上浮颗粒依次穿过连接导管227和喷嘴壁229。这使来自环形增压室的输入流因喷嘴壁施加的向内径向运动而使上升的聚集颗粒溢出。这允许上升颗粒也捕获输入流中的较小颗粒，从而提高洗涤效果。因此连接导管227和波状喷嘴壁229的长度提高了洗涤效果。发现对于尺寸为0.1微米至20微米的颗粒(使用常规方法具有非常低的效率)而言具有特别高的效力。
图7的装置成形为：使得流体经由装置入口流动到装置中，进入声腔的第一端，然后并行地流动穿过第一流动腔和第二流动腔，经由声腔的第二端离开声腔，并经由装置出口离开装置。该设计在通往流动腔室128的入口处提供了具有低流动紊流的优化的速度分布，流动腔之前的洗涤长度能够在声学分离之前提高颗粒聚集和/或聚结，并且收集旋涡的使用能够在收集导管233处帮助除去颗粒。
图9示出了可以包含双声电泳腔的替代装置设计。在该装置中，入口和出口这两者位于装置的相同端(在这里为上端)。经由进入端口332泵入流体/颗粒混合物。混合物借助于重力向下流动穿过声腔，在声腔中，超声驻波捕获且保持颗粒。随着流体被持续泵入到流体路径中，最终装置充满流体，并且流体压力上升到足够高，使得流体将经由装置顶部处的出口334流出。超声驻波内的颗粒凝聚或聚集，并最终变大至重力克服了驻波的声学力的尺寸，并且颗粒聚集，然后掉落/下沉到收集井部310中，该收集井部310的横截面尺寸向下渐缩至顶点312。排出管线314将顶点312与端口316连接起来，在端口316处可以将集中在一起的颗粒从井部中取出。
如这里所示，入口332与出口334之间的流动通道330中设置有壁部340。因此，流体从入口经由第一流动腔向下流动穿过声腔，然后经由第二流动腔向上返回，并随后流动至出口。第一流动腔的横截面面积可以小于、等于或大于第二流动腔的横截面面积。如这里所示，壁部(保持板是壁部的一部分)放置为使得对进入端口而言的流动通道的横截面面积341小于对离开端口而言的流动通道的横截面面积343。
与入口相邻的第一保持壁342以及与出口相邻的第二保持壁344也是可见的。如这里所看到的，入口332和出口334设置为与前壁中部相对接近，并与装置的上端304间隔开。输入流体在流动到声腔中之前必须朝上端304流动，并随后从第一保持壁342溢出。类似地，从声腔返回的流体在经由出口334离开之前必须从第二保持壁344溢出。该构造提供了如下方法：借助于该方法，可以减少输入流体的紊流，使得声腔的声驻波中所捕获的颗粒在聚集至足够尺寸之前不会被瓦解或从驻波中清洗出去。
同样如这里所示，在一些实施例中，壁部340与壳体的上端304间隔开。例如，在流动路径被意外阻塞的情况下，该间隙346形成且用作入口332与出口334之间的卸压通道。
这种构造的结果是，流体经由装置入口流动到装置中，然后沿从声腔的第一端经由第一流动腔至第二端接着经由第二流动腔返回至第一端的U形路径行进通过声腔，然后经由声腔的第一端离开流动腔，并经由装置出口离开装置。
包含压电元件的声腔的各种部件可以由例如聚碳酸酯、丙烯酸(例如，聚甲基丙烯酸甲酯)或玻璃(例如，碱石灰或硼硅酸盐)或聚丙烯等任意合适材料制成。总体上要求材料稍微透明，使得可以制作出洁净的窗部，并且在声电泳装置/系统的操作期间可以看到内部流动通道和流动路径。
可以在组件的内部流动通道上使用各种涂层。这种涂层包括：环氧树脂，例如，与胺或聚酰胺交联的环氧氯丙烷双酚A；或聚氨酯涂层，例如，与脂肪族异氰酸酯交联的聚酯多元醇；或硅树脂涂层或聚氧化烯涂层。此类涂层对生产光滑表面和/或减小表面张力、允许细胞在重力作用下更好地沿着流动通道表面滑动并进入到所需位置(例如收集井部)比较有用。
声电泳装置的流量必须受控，以便重力可以作用在颗粒聚合体上。就这一点而言，可以设想的是，在声电泳装置中进入/离开流动路径的颗粒/流体混合物可以以高达约100毫升每分钟(ml/min)的流量流动。
在本系统中，以如下电压操作该系统：该电压使得颗粒被捕获在超声驻波中，即，保持在固定位置中。颗粒被收集在以半个波长隔开的明确限定的捕获线中。在各波节面中，以最小的声辐射电位捕获颗粒。声辐射力的轴向分量将具有正对比系数的颗粒驱动至压力波节面，而具有负对比系数的颗粒驱动至压力波腹面。声辐射力的径向或横向分量是捕获颗粒的力。声辐射力的径向或横向分量与声辐射力的轴向分量处于相同的数量级。如上文所讨论的，与压电元件如具有均一位移的活塞那样有效地移动的振动形式相反，可以通过以高阶振形驱动压电元件来增加横向力。声压与驱动电压成正比。电功率与电压的平方成正比。
在实施例中，驱动压电元件的脉冲电压信号可以具有正弦、方形、锯齿或三角形波形；并具有500kHz至10MHz的频率。可以用脉冲宽度调制来驱动脉冲电压信号，脉宽调制能够产生任意所需的波形。脉冲电压信号还可以具有幅度调制或频率调制开启/停止能力，以消除流动。
压电晶体的尺寸、形状和厚度决定了在不同频率的激励下的位移，这反过来又影响分离效率。通常来说，压电元件在接近厚度谐振频率(半波长)的频率下工作。位移的梯度通常形成用于要被捕获的颗粒的更多地点。高阶模态位移产生在声场中的所有方向上具有强梯度的三维声驻波，从而在所有方向上产生相同强度的声辐射力，从而形成多条捕获线，这里，捕获线的数量与压电元件的特定振形相关。
图10是线性对数曲线图(线性y轴，对数x轴)，该曲线图示出了声辐射力、流体曳力和浮力与颗粒半径的比例关系。完成了对实验中所使用的典型SAE-30油滴的计算。浮力是颗粒容积有关力，因此，对近似于微米级的粒度而言该浮力是可忽略的，但是对处于几百微米级的粒度而言该浮力渐渐变得重要。流体曳力与流体速度成线性比例，因此对微米尺寸级的颗粒而言流体曳力通常超过浮力，但是对处于几百微米级的较大尺寸颗粒而言该流体曳力是可忽略的。声辐射力比例作用不同。当粒度较小时，声捕获力与颗粒体积成比例。最终，当粒度变大时，声辐射力不再随着颗粒半径的立方增大，并且在一定临界粒度下将快速消失。为了进一步增大粒度，辐射力在幅值上再次增大但具有相反的相位(曲线图中未示出)。重复该模式以增大粒度。
起初，当悬浮液主要与小微米尺寸级颗粒一起流动穿过系统时，对要被捕获在驻波中的颗粒而言，需要声辐射力平衡流体曳力和浮力的组合作用。在图10中，对约3.5微米的粒度(标记为Rc1)而言发生以上内容。然后，曲线图示出了还将捕获所有较大颗粒。因此，当在驻波中捕获小颗粒时，发生颗粒聚结/凝结/聚合/聚集，从而导致有效粒度持续变大。随着粒度变大，颗粒反射声辐射力，使得大颗粒将使声辐射力减小。粒度持续变大直到浮力变得占优势(这用第二临界粒度Rc2表示)为止，在第二临界粒度Rc2处，颗粒将基于相对于主流体的相对密度上升或下沉。随着颗粒上升或下沉，这些颗粒不再反射声辐射力，使得声辐射力随后增大。并非所有颗粒将掉落，并且这些残留颗粒的尺寸也将持续变大。该现象说明了在大于尺寸Rc2的情况下声辐射力的快速下降和上升。因此，图10说明了小颗粒如何在驻波中被持续捕获，变大成较大颗粒或块体，并然后最终因增大的浮力而上升或沉积。
下述实例用于进一步说明本发明。这些实例仅是说明性的，并且不意图将根据本发明制成的装置限于本文所述的材料、条件或工艺参数。
实例
使用常规声电泳装置作为比较例。该装置使用如下声腔：在声腔中，腔的一个壁部上设置有超声换能器，而相对壁部上设置有反射体。该换能器具有一个尺寸为1英寸×3英寸的压电晶体。
本发明的声电泳装置标记为实例。该装置使用如下声腔：声腔中部安装有压电晶体。两个反射体位于相反壁部上，且与一个压电晶体的表面平行。该压电晶体也具有1英寸×3英寸的尺寸。
然后，在酵母泥作为供给输入的情况下操作两个装置。该酵母泥含有0.5％的固体。装置运行超过40分钟。图11是示出了双声电泳腔的操作的图片。由位于腔的中部的压电元件所产生的捕获线是可见的。
定期从声腔中取出样品。在运行40分钟之后，还测量浓缩物、渗透物和保留物。浓缩物是与一些流体一起离开装置且含有被浓缩的酵母的部分。渗透物是离开装置的已过滤部分，该已过滤部分主要是具有非常低浓度的酵母的液体。保留物是在运行之后留在装置中的残留物质。下述表格中提供了结果。
比较例 实例
供给物浊度(NTU) 876 978
保留物浊度(NTU) 1345 1708
浓缩物浊度(NTU) 3620 2164
渗透物浊度(NTU) 71.6 88.1
总效率(％) 92 91
可以从这些结果看到的是，双声电泳腔(其中，压电元件位于腔的容积中并将腔分成多个流动腔)的效率与比较例所示的常规系统的效率大致相等。
图12是示出了对来自供给物的浊度的降低百分比而言的随着时间推移的效率的图表。如这里所看到的，本装置正好与常规系统一样随着时间推移高效且有效。
已经参考示例性实施例描述了本发明。显然，在阅读并理解了前述详细说明后，其他人将能够对其进行修改和变更。其意在本发明应被解释为包括所有此类修改和变更，只要它们在所附权利要求书或其等同内容的范围之内即可。

Claims (20)

1.一种声电泳装置，包括：
装置入口，其允许流体流进入到所述声电泳装置中；
装置出口，其允许流体从所述声电泳装置流出；以及
声腔，其位于所述装置入口与所述装置出口之间的流动路径中，所述声腔包括：
第一端以及与所述第一端相对的第二端；
第一压电元件，其在所述第一端与所述第二端之间延伸，所述第一压电元件具有第一表面和第二表面；
第一反射体，其与所述第一压电元件的所述第一表面相对，所述第一反射体与所述第一压电元件的所述第一表面之间设置有第一流动腔；以及
第二反射体，其与所述第一压电元件的所述第二表面相对，所述第二反射体与所述第一压电元件的所述第二表面之间设置有第二流动腔。
2.根据权利要求1所述的声电泳装置，其中，所述声腔还包括：保持板，其保持所述压电元件；以及两个支架板，其具有狭槽，所述狭槽用于将所述保持板保持在所述声腔中的固定位置。
3.根据权利要求1所述的声电泳装置，其中，所述压电元件包括多个压电晶体。
4.根据权利要求1所述的声电泳装置，其中，所述压电元件适合于在所述第一流动腔和所述第二流动腔中产生多维驻波。
5.根据权利要求4所述的声电泳装置，其中，在所述第一流动腔和所述第二流动腔中由所述压电元件产生不同的驻波。
6.根据权利要求1所述的声电泳装置，其中，所述声电泳装置成形为：使得流体经由所述装置入口流动到所述声电泳装置中，进入所述声腔的所述第一端中，然后并行地流动穿过所述第一流动腔和所述第二流动腔，经由所述声腔的所述第二端从所述声腔流出，并经由所述装置出口从所述声电泳装置流出。
7.根据权利要求1所述的声电泳装置，还包括：
波状喷嘴壁，其位于所述装置入口与所述声腔之间。
8.根据权利要求1所述的声电泳装置，其中，所述声电泳装置成形为：使得流体经由所述装置入口流动到所述声电泳装置中，然后沿从所述声腔的所述第一端经由所述第一流动腔至所述第二端接着经由所述第二流动腔返回至所述第一端的U形路径行进通过所述声腔，然后经由所述声腔的所述第一端离开流动腔，并经由所述装置出口离开所述声电泳装置。
9.根据权利要求8所述的声电泳装置，其中，所述声腔的所述第二端通向井部，所述井部的横截面面积从单个入口向下渐缩至顶点，并且排出管线将所述顶点连接至用于回收在所述井部中收集的材料的端口。
10.根据权利要求1所述的声电泳装置，还包括：
第二压电元件，其在所述第一端与所述第二端之间延伸，所述第二压电元件具有第一表面和第二表面，所述第二压电元件的所述第一表面面向所述第二反射体，并且所述第二反射体与所述第二压电元件的所述第一表面之间设置有第三流动腔；以及
第三反射体，其与所述第二压电元件的所述第二表面相对，所述第三反射体与所述第二压电元件的所述第二表面之间设置有第四流动腔。
11.一种使颗粒与主流体分离的方法，包括：
使所述主流体和所述颗粒的混合物流动穿过声电泳装置，所述声电泳装置包括：
装置入口，其允许流体流进入到所述声电泳装置中；
装置出口，其允许流体从所述声电泳装置流出；以及
声腔，其位于所述装置入口与所述装置出口之间的流动路径中，所述声腔包括：
第一端以及与所述第一端相对的第二端；
第一压电元件，其在所述第一端与所述第二端之间延伸，所述第一压电元件具有第一表面和第二表面；
第一反射体，其与所述第一压电元件的所述第一表面相对，所述第一反射体与所述第一表面之间设置有第一流动腔；以及
第二反射体，其与所述第一压电元件的所述第二表面相对，所述第二反射体与所述第二表面之间设置有第二流动腔；以及
发送脉冲电压信号，以驱动所述压电元件在所述第一流动腔和所述第二流动腔中产生多维驻波，从而使所述颗粒与所述主流体分离。
12.根据权利要求11所述的方法，其中，在所述第一流动腔和所述第二流动腔这两者中，所述多维驻波产生具有轴向力分量和横向力分量的声辐射力，所述轴向力分量和所述横向力分量具有相同的数量级。
13.根据权利要求11所述的方法，其中，所述颗粒是中国仓鼠卵巢(CHO)细胞、NS0杂交瘤细胞、幼仑鼠肾(BHK)细胞或人体细胞。
14.根据权利要求11所述的方法，其中，所述脉冲电压信号具有500kHz至10MHz的频率。
15.根据权利要求11所述的方法，其中，利用幅度或频率调制开启/停止能力驱动所述脉冲电压信号，以消除声流。
16.根据权利要求11所述的方法，其中，所述主流体和所述颗粒的所述混合物在进入所述声腔之前具有1500以下的雷诺数。
17.根据权利要求11所述的方法，其中，在所述第一流动腔和所述第二流动腔中由所述压电元件产生不同的驻波。
18.根据权利要求11所述的方法，其中，所述声电泳装置成形为：使得流体经由所述装置入口流动到所述声电泳装置中，进入所述声腔的所述第一端中，然后并行地流动穿过所述第一流动腔和所述第二流动腔，经由所述声腔的所述第二端从所述声腔流出，并经由所述装置出口从所述声电泳装置流出。
19.根据权利要求11所述的方法，其中，所述声电泳装置成形为：使得流体经由所述装置入口流动到所述声电泳装置中，然后沿从所述声腔的所述第一端经由所述第一流动腔至所述第二端接着经由所述第二流动腔返回至所述第一端的U形路径行进通过所述声腔，然后经由所述声腔的第一端离开流动腔，并经由所述装置出口离开所述声电泳装置。
20.一种声电泳装置，包括：
装置入口，其允许流体流进入到所述声电泳装置中；
装置出口，其允许流体从所述声电泳装置流出；以及
声腔，其位于所述装置入口与所述装置出口之间的流动路径中，所述声腔包括：
第一端以及与所述第一端相对的第二端；
多个压电元件，其在所述第一端与所述第二端之间延伸，每个所述压电元件均具有第一表面和第二表面；以及
多个反射体；
其中，每个所述压电元件均位于两个反射体之间。
CN201580004104.2A 2014-01-08 2015-01-08 具有双声电泳腔的声电泳装置 Active CN105939767B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461925171P 2014-01-08 2014-01-08
US61/925,171 2014-01-08
PCT/US2015/010595 WO2015105955A1 (en) 2014-01-08 2015-01-08 Acoustophoresis device with dual acoustophoretic chamber

Publications (2)

Publication Number Publication Date
CN105939767A true CN105939767A (zh) 2016-09-14
CN105939767B CN105939767B (zh) 2018-04-06

Family

ID=52394407

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580004104.2A Active CN105939767B (zh) 2014-01-08 2015-01-08 具有双声电泳腔的声电泳装置

Country Status (5)

Country Link
US (2) US9725710B2 (zh)
EP (1) EP3092049A1 (zh)
CN (1) CN105939767B (zh)
CA (1) CA2935960C (zh)
WO (1) WO2015105955A1 (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108449987A (zh) * 2015-11-24 2018-08-24 保罗·谢勒学院 通过收集x射线衍射图像以原子分辨率解析晶体的晶体结构的系统和方法
CN111821601A (zh) * 2020-05-27 2020-10-27 深圳艾素科技有限公司 一种电子过滤装置及其操作方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10704021B2 (en) * 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
CA2984492A1 (en) 2015-04-29 2016-11-03 Flodesign Sonics, Inc. Acoustophoretic device for angled wave particle deflection
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
EP3725092A4 (en) 2017-12-14 2021-09-22 FloDesign Sonics, Inc. DRIVE AND CONTROL UNIT FOR ACOUSTIC CONVERTER
EP3501619B1 (en) * 2017-12-20 2023-10-18 Nokia Technologies Oy Apparatus for collecting particles within a fluid
EP3788362A1 (en) 2018-04-30 2021-03-10 Teknologian Tutkimuskeskus VTT Oy Trapping and releasing particles carried by gas sample

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990005008A1 (de) * 1988-11-03 1990-05-17 Ewald Benes Verfahren und einrichtung zur separation von teilchen
US5711888A (en) * 1993-05-11 1998-01-27 Sonosep Biotech, Inc. Multilayered piezoelectric resonator for the separation of suspended particles
US20120328447A1 (en) * 2011-06-24 2012-12-27 Alstom Technology Ltd Blade of a turbomachine
US20130277317A1 (en) * 2012-04-20 2013-10-24 Flodesign Sonics Inc. Acoustophoretic enhanced system for use in tanks

Family Cites Families (823)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2473971A (en) 1944-02-25 1949-06-21 Donald E Ross Underwater transducer
US2667944A (en) 1949-12-10 1954-02-02 Combustion Eng Cyclone separator
US3372370A (en) 1965-09-22 1968-03-05 Aquasonics Engineering Company Electroacoustic transducer
US3555311A (en) 1969-01-23 1971-01-12 Marquardt Corp High pressure piezoelectric transducer
US4158629A (en) 1974-08-12 1979-06-19 Vernon D. Beehler Dynamic self-cleaning filter for liquids
US4204096A (en) 1974-12-02 1980-05-20 Barcus Lester M Sonic transducer mounting
US4055491A (en) 1976-06-02 1977-10-25 Porath Furedi Asher Apparatus and method for removing fine particles from a liquid medium by ultrasonic waves
SU629496A1 (ru) 1976-08-01 1978-10-25 Институт Радиофизики И Электроники Ан Украинской Сср Акустический преобразователь ультразвука
US4065875A (en) 1976-09-17 1978-01-03 University Of Delaware Selective destruction of certain algae
JPS6034433B2 (ja) 1977-03-07 1985-08-08 株式会社豊田中央研究所 超音波変換器
US4118649A (en) 1977-05-25 1978-10-03 Rca Corporation Transducer assembly for megasonic cleaning
US4125789A (en) 1977-06-07 1978-11-14 Sundstrand Corporation Biasing and scaling circuit for transducers
US4165273A (en) 1977-12-20 1979-08-21 Azarov Nikolai N Device for producing deeply desalted water
US4320659A (en) 1978-02-27 1982-03-23 Panametrics, Inc. Ultrasonic system for measuring fluid impedance or liquid level
AU529113B2 (en) 1978-04-19 1983-05-26 Commonwealth Of Australia, The Ultrasonic transducer array
US4211949A (en) 1978-11-08 1980-07-08 General Electric Company Wear plate for piezoelectric ultrasonic transducer arrays
CA1141629A (en) 1979-07-31 1983-02-22 Roger F. Potts Machine for cleaning plastic containers
FR2485858B1 (fr) 1980-06-25 1986-04-11 Commissariat Energie Atomique Procede de fabrication de transducteurs ultrasonores de formes complexes et application a l'obtention de transducteurs annulaires
DE3027433A1 (de) 1980-07-19 1982-02-18 Messerschmitt-Bölkow-Blohm GmbH, 8000 München Verfahren zur trennung von gemischen
US5834871A (en) 1996-08-05 1998-11-10 Puskas; William L. Apparatus and methods for cleaning and/or processing delicate parts
DE3218488A1 (de) 1982-05-15 1983-11-17 Battelle-Institut E.V., 6000 Frankfurt Verfahren und vorrichtung zum sortieren von partikeln nach unterschiedlichen dichtebereichen
US4552669A (en) 1983-04-18 1985-11-12 Mott Metallurgical Corporation Pneumatic hydro-pulse filter system and method of operation
US4484907A (en) 1983-10-20 1984-11-27 E. I. Du Pont De Nemours And Company Microtube adapter having a holding and uncapping apparatus
US4743361A (en) 1983-10-31 1988-05-10 Internationale Octrooi Maatschappij "Octropa" Bv Manipulation of particles
GB8417240D0 (en) 1984-07-06 1984-08-08 Unilever Plc Particle separation
EP0278108B1 (de) 1984-07-18 1989-08-16 Schenck Auto-Service-Geräte GmbH Verfahren und Vorrichtung zum Stillsetzen eines rotierenden Körpers
CN85100483B (zh) 1985-04-01 1988-10-19 上海灯泡厂 超声波换能器用背载材料
US4666595A (en) 1985-09-16 1987-05-19 Coulter Electronics, Inc. Apparatus for acoustically removing particles from a magnetic separation matrix
US4759775A (en) 1986-02-21 1988-07-26 Utah Bioresearch, Inc. Methods and apparatus for moving and separating materials exhibiting different physical properties
US4983189A (en) 1986-02-21 1991-01-08 Technical Research Associates, Inc. Methods and apparatus for moving and separating materials exhibiting different physical properties
US4699588A (en) 1986-03-06 1987-10-13 Sonotech, Inc. Method and apparatus for conducting a process in a pulsating environment
GB8612759D0 (en) 1986-05-27 1986-07-02 Unilever Plc Manipulating particulate matter
BE905707A (nl) 1986-11-06 1987-03-02 Poppe Willy Dekbed.
AT389235B (de) 1987-05-19 1989-11-10 Stuckart Wolfgang Verfahren zur reinigung von fluessigkeiten mittels ultraschall und vorrichtungen zur durchfuehrung dieses verfahrens
US4821838A (en) 1987-10-30 1989-04-18 Hewlett-Packard Company Acoustic damper
JPH01134216A (ja) 1987-11-19 1989-05-26 Agency Of Ind Science & Technol ３次元定在波音場の可視化方法
US4860993A (en) 1988-01-14 1989-08-29 Teledyne Industries, Inc. Valve design to reduce cavitation and noise
US4836684A (en) 1988-02-18 1989-06-06 Ultrasonic Power Corporation Ultrasonic cleaning apparatus with phase diversifier
WO1989011899A1 (en) 1988-06-08 1989-12-14 Invitron Corporation Tandem hollow fiber cell culture product harvest system
FR2638659B1 (fr) 1988-11-07 1992-06-12 Framatome Sa Appareil de filtration comportant un dispositif de decolmatage par ultrasons et procede de decolmatage correspondant
JPH02290266A (ja) 1989-04-28 1990-11-30 Mitsubishi Heavy Ind Ltd 超音波式原油脱水・脱塩装置
GB9005705D0 (en) 1990-03-14 1990-05-09 Health Lab Service Board Particle manipulation
US5085783A (en) 1990-08-16 1992-02-04 Case Western Reserve University Acoustically driven particle separation method and apparatus
US5059811A (en) 1990-08-30 1991-10-22 Great Lakes Instruments, Inc. Turbidimeter having a baffle assembly for removing entrained gas
RU2085933C1 (ru) 1991-08-14 1997-07-27 Кирпиченко Борис Иванович Устройство для ультразвукового контроля плотности раствора
RU2037327C1 (ru) 1991-12-09 1995-06-19 Александр Александрович Ковалев Ультразвуковой фильтр
GB2265004B (en) 1992-03-10 1996-01-10 Univ Cardiff Immuno-agglutination assay using ultrasonic standing wave field
CA2133823C (en) 1992-04-06 2002-10-15 Norman D.G. Mountford Ultrasonic treatment of liquids in particular metal melts
US6216538B1 (en) 1992-12-02 2001-04-17 Hitachi, Ltd. Particle handling apparatus for handling particles in fluid by acoustic radiation pressure
AT398707B (de) 1993-05-11 1995-01-25 Trampler Felix Mehrschichtiger piezoelektrischer resonator für die separation von suspendierten teilchen
US5626767A (en) 1993-07-02 1997-05-06 Sonosep Biotech Inc. Acoustic filter for separating and recycling suspended particles
US5443985A (en) 1993-07-22 1995-08-22 Alberta Research Council Cell culture bioreactor
AU688334B2 (en) 1993-09-07 1998-03-12 Siemens Medical Solutions Usa, Inc. Broadband phased array transducer design with frequency controlled two dimension capability and methods for manufacture thereof
US5431817A (en) 1993-09-13 1995-07-11 W. R. Grace & Co.-Conn. Bleach resistant polysulfone/polyurethane composite membranes
US5371429A (en) 1993-09-28 1994-12-06 Misonix, Inc. Electromechanical transducer device
US5395592A (en) 1993-10-04 1995-03-07 Bolleman; Brent Acoustic liquid processing device
US5452267A (en) 1994-01-27 1995-09-19 Magnetrol International, Inc. Midrange ultrasonic transducer
US5562823A (en) 1994-04-25 1996-10-08 Reeves; William Combination centrifugal and sonic device for separating components within a solution
EP0764265A4 (en) 1994-06-07 1998-05-20 Univ Boston ACOUSTIC DETECTION OF PARTICLES
US5560362A (en) 1994-06-13 1996-10-01 Acuson Corporation Active thermal control of ultrasound transducers
US5475486A (en) 1994-10-21 1995-12-12 Hach Company Flow cell system for turbidimeter
JP3487699B2 (ja) 1995-11-08 2004-01-19 株式会社日立製作所 超音波処理方法および装置
JP3875736B2 (ja) 1995-11-14 2007-01-31 株式会社協和エクシオ 排水処理方法および装置
JP2700058B2 (ja) 1996-01-23 1998-01-19 工業技術院長 超音波を用いた非接触マイクロマニピュレーション方法
US6641708B1 (en) 1996-01-31 2003-11-04 Board Of Regents, The University Of Texas System Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation
US5688405A (en) 1996-02-28 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Method and apparatus for separating particulate matter from a fluid
AU2318597A (en) 1996-03-19 1997-10-10 Ozone Sterilization Products, Inc. Ozone sterilizer and generator
NL1003595C2 (nl) 1996-04-10 1997-10-14 Tno Werkwijze en inrichting voor het karakteriseren van suspensies.
US5844140A (en) 1996-08-27 1998-12-01 Seale; Joseph B. Ultrasound beam alignment servo
MX9604952A (es) 1996-10-18 1998-04-30 Servicios Condumex Sa Clasificador y reactor hidraulico de particulas solidas con aplicacion de ultrasonido.
GB9621832D0 (en) 1996-10-19 1996-12-11 Univ Cardiff Removing partiles from suspension
DE19648519C2 (de) 1996-11-23 2000-11-16 Preussag Wassertechnik Gmbh Verfahren und Anlage zur Stofftrennung mittels Membranfiltration
GB9708984D0 (en) * 1997-05-03 1997-06-25 Univ Cardiff Particle manipulation
US5951456A (en) 1997-05-16 1999-09-14 Scott; Harold W. Ultrasonic methods and apparatus for separating materials in a fluid mixture
JPH1190110A (ja) 1997-09-16 1999-04-06 Konica Corp 超音波脱泡方法、感光材料の生産方法及び超音波脱泡装置
US6029518A (en) 1997-09-17 2000-02-29 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Manipulation of liquids using phased array generation of acoustic radiation pressure
US6280388B1 (en) 1997-11-19 2001-08-28 Boston Scientific Technology, Inc. Aerogel backed ultrasound transducer
US6326213B1 (en) 1998-02-09 2001-12-04 The Boards Of Govenors For Higher Education, State Of Rhode Island And Providence Plantations Acoustic standing-wave enhancement of a fiber-optic Salmonella biosensor
DE19815882A1 (de) 1998-04-08 1999-10-14 Fuhr Guenther Verfahren und Vorrichtung zur Manipulierung von Mikropartikeln in Fluidströmungen
DE19820466C2 (de) 1998-05-07 2002-06-13 Fraunhofer Ges Forschung Vorrichtung und Verfahren zur gezielten Beaufschlagung einer biologischen Probe mit Schallwellen
JP2000024431A (ja) 1998-07-14 2000-01-25 Hitachi Ltd 微粒子処理装置
US6090295A (en) 1998-08-11 2000-07-18 University Technology Corporation Method and apparatus for acoustically demixing aqueous solutions
US7981368B2 (en) 1998-10-28 2011-07-19 Covaris, Inc. Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
CN1286668A (zh) 1998-11-20 2001-03-07 普拉乌道株式会社 液体处理方法、液体处理装置及液体处理系统
US6166231A (en) 1998-12-15 2000-12-26 Martek Biosciences Corporation Two phase extraction of oil from biomass
WO2000041794A1 (en) 1999-01-15 2000-07-20 University College Cardiff Consultants Ltd. Particle manipulation
CN1181337C (zh) 2000-08-08 2004-12-22 清华大学 微流体系统中实体分子的操纵方法及相关试剂盒
US6592821B1 (en) 1999-05-17 2003-07-15 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
US6314974B1 (en) 1999-06-28 2001-11-13 Fairchild Semiconductor Corporation Potted transducer array with matching network in a multiple pass configuration
GB9916851D0 (en) 1999-07-20 1999-09-22 Univ Wales Bangor Manipulation of particles in liquid media
AUPQ629100A0 (en) 2000-03-16 2000-04-15 Btf Pty Ltd Process for preparing controlled samples of particles such as microorganisms and cells
AU2001263213B2 (en) 2000-05-16 2005-05-19 Atrionix, Inc. Apparatus and method incorporating an ultrasound transducer onto a delivery member
US6395186B1 (en) 2000-06-20 2002-05-28 Delaware Capital Formation, Inc. Pressure liquid filtration with ultrasonic bridging prevention
EP1175931A1 (en) 2000-07-25 2002-01-30 Computer Cell Culture Center S.A. Integration of high cell density bioreactor operation with ultra fast on-line downstream processing
AU2001288447A1 (en) 2000-08-28 2002-03-13 Ocean Power Corporation Method for pretreating water for desalination
CN100495030C (zh) 2000-09-30 2009-06-03 清华大学 多力操纵装置及其应用
JP2004516468A (ja) 2000-12-18 2004-06-03 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 懸濁中の粒子の超音波サイズ測定方法および装置
SE0103013D0 (sv) 2001-03-09 2001-09-12 Erysave Ab Ideon System and method for treatment of whole blood
SE522801C2 (sv) 2001-03-09 2004-03-09 Erysave Ab Anordning för att separera suspenderade partiklar från en fluid med ultraljud samt metod för sådan separering
SE0100820D0 (sv) 2001-03-09 2001-03-09 Erysave Ab Ideon Particle separation using an acoustic filter
US6467350B1 (en) 2001-03-15 2002-10-22 The Regents Of The University Of California Cylindrical acoustic levitator/concentrator
US6770248B2 (en) 2001-05-04 2004-08-03 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence Of Her Majesty's Canadian Government Flowthrough device for the ultrasonic destruction of microorganisms in fluids
US6763722B2 (en) 2001-07-13 2004-07-20 Transurgical, Inc. Ultrasonic transducers
US6551248B2 (en) 2001-07-31 2003-04-22 Koninklijke Philips Electronics N.V. System for attaching an acoustic element to an integrated circuit
DE10148916A1 (de) 2001-10-04 2003-04-17 Beatrix Christa Meier Ultraschallvorrichtung
US6487095B1 (en) 2001-10-31 2002-11-26 International Business Machines Corporation Multiphase zero-volt-switching resonant DC-DC regulator
US7470545B2 (en) 2001-11-05 2008-12-30 Rohm And Haas Company Buccal dissolution of active substances
AU2002343107A1 (en) 2001-11-27 2003-06-10 Euroflow (Uk) Limited Detection systems and methods
US6649069B2 (en) 2002-01-23 2003-11-18 Bae Systems Information And Electronic Systems Integration Inc Active acoustic piping
JP3633926B2 (ja) 2002-01-28 2005-03-30 松下電器産業株式会社 超音波送受信器および超音波流量計
EP1354941A1 (en) 2002-04-19 2003-10-22 Computer Cell Culture Center S.A. Apparatus and method for a cell culture in a bioreactor at high cell concentration
US6749666B2 (en) 2002-04-26 2004-06-15 Board Of Regents, The University Of Texas System Modulated acoustic aggiomeration system and method
KR100571803B1 (ko) 2002-05-03 2006-04-17 삼성전자주식회사 수소로 기능화된 반도체 탄소나노튜브를 포함하는 전자 소자 및 그 제조방법
AU2003240739A1 (en) 2002-06-03 2003-12-19 Shell Internationale Research Maatschappij B.V. Downhole desalination of aquifer water
US7846382B2 (en) 2002-06-04 2010-12-07 Protasis Corporation Method and device for ultrasonically manipulating particles within a fluid
US20040016699A1 (en) 2002-07-29 2004-01-29 Michael Bayevsky Systems and methods for ultrasonic cleaning of cross-flow membrane filters
US6938488B2 (en) 2002-08-21 2005-09-06 Battelle Memorial Institute Acoustic inspection device
GB0221391D0 (en) 2002-09-16 2002-10-23 Secr Defence Apparatus for directing particles in a fluid
US6881389B2 (en) 2002-09-24 2005-04-19 Edg, Inc. Removal of H2S and CO2 from a hydrocarbon fluid stream
GB0222421D0 (en) 2002-09-27 2002-11-06 Ratcliff Henry K Advanced ultrasonic processor
US7108137B2 (en) 2002-10-02 2006-09-19 Wisconsin Alumni Research Foundation Method and apparatus for separating particles by size
EP1565245A4 (en) 2002-11-01 2006-06-07 Univ Texas ACOUSTIC STIMULATION OF A STEAM DIFFUSION SYSTEM AND METHOD THEREOF
US6878288B2 (en) 2002-12-17 2005-04-12 Harold W. Scott System and apparatus for removing dissolved and suspended solids from a fluid stream
US7191787B1 (en) 2003-02-03 2007-03-20 Lam Research Corporation Method and apparatus for semiconductor wafer cleaning using high-frequency acoustic energy with supercritical fluid
SE0300290D0 (sv) 2003-02-05 2003-02-05 Siemens Elema Ab Acoustic meter assembly
AU2003270259A1 (en) 2003-03-06 2004-09-28 Eth Zurich Method for positioning small particles in a fluid
US7008540B1 (en) 2003-04-07 2006-03-07 The Ohio State University Ultrasonically cleaned membrane filtration system
DE10319467B3 (de) 2003-04-29 2004-07-22 Miele & Cie. Kg Einrichtung mit einem Trübungssensor für eine Geschirrspülmaschine oder Waschmaschine
US6990852B2 (en) 2003-07-28 2006-01-31 Becton Dickinson & Company System and method for detecting particles
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
CN1245231C (zh) 2003-08-27 2006-03-15 中国石油化工股份有限公司 顺流和逆流超声波联合作用使油水乳化物破乳的方法及装置
US7541166B2 (en) 2003-09-19 2009-06-02 Microfluidic Systems, Inc. Sonication to selectively lyse different cell types
US7232016B2 (en) 2003-12-08 2007-06-19 General Motors Corporation Fluid damper having continuously variable damping response
US7445716B2 (en) 2004-01-05 2008-11-04 Eaton Lp Crossflow pressure liquid filtration with ultrasonic enhancement
US8012355B2 (en) 2004-01-30 2011-09-06 Pss Acquisitionco Llc Molecular separator
JP2005249267A (ja) 2004-03-03 2005-09-15 Sanyo Electric Co Ltd 電子レンジ
US7044737B2 (en) 2004-03-05 2006-05-16 Liang Fu Ultrasound oral hygiene and therapeutic device
BRPI0510291A (pt) 2004-04-27 2007-10-30 Baxter Int sistema de reator com tanque agitado
EP1766608B1 (en) 2004-06-07 2017-08-09 Koninklijke Philips N.V. Acoustic device with variable focal length
US7340957B2 (en) 2004-07-29 2008-03-11 Los Alamos National Security, Llc Ultrasonic analyte concentration and application in flow cytometry
US20080272034A1 (en) 2004-08-16 2008-11-06 Searete Llc, Separation of particles from a fluid by wave action
AT413655B (de) 2004-08-19 2006-04-15 Felix Dipl Ing Dr Trampler Vorrichtung zur abscheidung von dispergierten partikeln
US7230882B2 (en) 2004-09-03 2007-06-12 Lowrance Electronics, Inc. Transducer support and associated lock
SE528313C2 (sv) 2004-09-24 2006-10-17 Spectronic Ab Metod och apparat för separering av partiklar med hjälp av ultraljudvågor
GB2420510B (en) 2004-11-25 2010-10-06 Cyclotech Ltd Methods and apparatus for conditioning and degassing liquids and gases in suspension
WO2006069181A2 (en) 2004-12-21 2006-06-29 Musc Foundation For Research Development Compositions and methods for promoting wound healing and tissue regeneration
CA2593374A1 (en) 2004-12-29 2006-07-06 Biogen Idec Ma Inc. Bioreactor process control system and method
FR2882939B1 (fr) 2005-03-11 2007-06-08 Centre Nat Rech Scient Dispositif de separation fluidique
ES2716874T3 (es) 2005-03-23 2019-06-17 Genmab As Anticuerpos contra cd38 para el tratamiento del mieloma múltiple
US7704743B2 (en) 2005-03-30 2010-04-27 Georgia Tech Research Corporation Electrosonic cell manipulation device and method of use thereof
JP4770251B2 (ja) 2005-04-25 2011-09-14 パナソニック株式会社 成分分離デバイスおよびこれを用いた成分の分離方法
WO2007016605A2 (en) 2005-08-01 2007-02-08 Covaris, Inc. An apparatus and a method for processing a sample using acoustic energy
DE102005050167B4 (de) 2005-10-19 2009-02-19 Advalytix Ag Konzentrationsverfahren, Konzentrationsvorrichtung und Reaktionsverfahren
US7766121B2 (en) 2005-12-20 2010-08-03 Cyclotech Limited Methods and apparatus for conditioning and degassing liquids and gases in suspension
WO2007083295A2 (en) 2006-01-19 2007-07-26 Yeda Research And Development Co. Ltd. Device and method for particle manipulation in fluid
BRPI0708470A2 (pt) 2006-03-14 2011-05-31 Merck & Co Inc processo para a produção de partìculas cristalinas de um composto ativo orgánico, e, composição farmacêutica
US20070224676A1 (en) 2006-03-21 2007-09-27 Becton, Dickinson And Company Expandable culture roller bottle
CN1843945A (zh) 2006-05-16 2006-10-11 徐小宁 利用射流技术的海水淡化处理系统
US20070267351A1 (en) 2006-05-22 2007-11-22 Traceguard Technologies Inc. Low-frequency acoustic waves for collecting and/or moving particles inside articles
US20080011693A1 (en) 2006-07-12 2008-01-17 Ming Li Self-cleaning waste-water device and method
US8075786B2 (en) 2006-09-05 2011-12-13 The Board Of Regents Of The University Of Oklahoma Acoustic/pressure wave-driven separation device
US7763177B2 (en) 2006-10-26 2010-07-27 Atlantium Technologies Ltd. System and method for ultrasonic cleaning of ultraviolet disinfection system
JP4984849B2 (ja) 2006-11-27 2012-07-25 パナソニック株式会社 成分分離デバイスと、この成分分離デバイスを用いた化学分析デバイス
US7673516B2 (en) 2006-12-28 2010-03-09 Kimberly-Clark Worldwide, Inc. Ultrasonic liquid treatment system
WO2008106403A2 (en) 2007-02-26 2008-09-04 Trustees Of Princeton University Turnable acoustic gradient index of refraction lens and system
CN101678250B (zh) 2007-03-02 2013-04-24 史密夫及内修公开有限公司 用于在生物样品过滤中通过超声、回洗和过滤器运动进行过滤器清洁的装置和方法
DE102008006501B4 (de) 2007-03-19 2013-05-16 Sonja Lauterborn Kombiniertes Ultraschall-Luft-Rückspülverfahren zur chemikalienfreien In- situ-Reinigung getauchter Membranen bei Rückspülung während des Betriebes
US8911460B2 (en) 2007-03-22 2014-12-16 Ethicon Endo-Surgery, Inc. Ultrasonic surgical instruments
EP2664916B1 (en) 2007-04-02 2017-02-08 Acoustic Cytometry Systems, Inc. Method for manipulating a fluid medium within a flow cell using acoustic focusing
US7837040B2 (en) 2007-04-09 2010-11-23 Los Alamos National Security, Llc Acoustic concentration of particles in fluid flow
US8083068B2 (en) 2007-04-09 2011-12-27 Los Alamos National Security, Llc Apparatus for separating particles utilizing engineered acoustic contrast capture particles
EP2145687B1 (en) 2007-05-15 2014-12-03 Panasonic Corporation Component separation device
US7889601B2 (en) 2007-06-19 2011-02-15 Lockheed Martin Corporation Lightweight acoustic array
DE102007030904A1 (de) 2007-07-03 2009-02-05 Pharis Biotec Gmbh Humanes zirkulierendes antivirales Albumin-Fragment (ALB-408) und seine Verwendung
US8808319B2 (en) 2007-07-27 2014-08-19 Ethicon Endo-Surgery, Inc. Surgical instruments
US8882791B2 (en) 2007-07-27 2014-11-11 Ethicon Endo-Surgery, Inc. Ultrasonic surgical instruments
US20090042253A1 (en) 2007-08-09 2009-02-12 Wyeth Use of perfusion to enhance production of fed-batch cell culture in bioreactors
GB0716047D0 (en) 2007-08-16 2007-09-26 Welding Inst Acoustic transducer assembley
US8563314B2 (en) 2007-09-27 2013-10-22 Sangamo Biosciences, Inc. Methods and compositions for modulating PD1
EP2796102B1 (en) 2007-10-05 2018-03-14 Ethicon LLC Ergonomic surgical instruments
JP5119848B2 (ja) 2007-10-12 2013-01-16 富士ゼロックス株式会社 マイクロリアクタ装置
US20100255573A1 (en) 2007-11-14 2010-10-07 Damian Joseph Peter Bond Extraction and purification of biologigal cells using ultrasound
US8096177B2 (en) 2007-11-19 2012-01-17 Petroleum Recovery Services Llc Fuel inventory monitoring system
ES2326109B1 (es) 2007-12-05 2010-06-25 Consejo Superior De Investigaciones Cientificas Microdispositivo de separacion y extraccion selectiva y no invasiva de particulas en suspensiones polidispersas, procedimiento de fabricacion y sus aplicaciones.
US8030290B2 (en) 2007-12-07 2011-10-04 City Of Hope Cell-type specific aptamer-siRNA delivery system for HIV-1 Therapy
US8266950B2 (en) 2007-12-19 2012-09-18 Los Alamos National Security, LLP Particle analysis in an acoustic cytometer
US8714014B2 (en) 2008-01-16 2014-05-06 Life Technologies Corporation System and method for acoustic focusing hardware and implementations
US9480935B2 (en) 2008-02-01 2016-11-01 Lawrence Livermore National Security, Llc Systems and methods for separating particles and/or substances from a sample fluid
KR20100128304A (ko) 2008-03-03 2010-12-07 쓰리엠 이노베이티브 프로퍼티즈 컴파니 가스 유동 시스템 내의 가청 음향 주파수 관리를 위한 방법
US8319398B2 (en) 2008-04-04 2012-11-27 Microsonic Systems Inc. Methods and systems to form high efficiency and uniform fresnel lens arrays for ultrasonic liquid manipulation
US8054145B2 (en) 2008-04-30 2011-11-08 Georgia Tech Research Corporation Phononic crystal wave structures
EP2285744A1 (en) 2008-05-27 2011-02-23 Kolmir Water Technologies Ltd. Apparatus and method for treatment of a contaminated water-based fluid
DE102008002210A1 (de) 2008-06-04 2009-12-10 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von Erythropoietin
CA2893175C (en) 2008-06-10 2016-09-06 Sangamo Biosciences, Inc. Methods and compositions for generation of bax- and bak-deficient cell lines
WO2009149519A1 (en) 2008-06-12 2009-12-17 Winwick Business Solutions Pty Ltd System for cultivation and processing of microorganisms and products therefrom
US7935259B2 (en) 2008-07-03 2011-05-03 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Filtering apparatus and method of use
EP2310486B1 (en) 2008-07-16 2017-01-04 kSep Systems, LLC Methods and systems for manipulating particles using a fluidized bed
MX2011000410A (es) 2008-07-18 2011-03-24 Prosonix Ltd Proceso para mejorar la cristalinidad de particulas de fluticasona.
US8387803B2 (en) 2008-08-26 2013-03-05 Ge Healthcare Bio-Sciences Ab Particle sorting
KR102178191B1 (ko) 2008-09-02 2020-11-13 머크 밀리포어 리미티드 크로마토그래피 막, 이를 포함하는 장치 및 이의 이용방법
JP2012501797A (ja) 2008-09-11 2012-01-26 ルナ イノベーションズ インコーポレイテッド 流体から気泡を音響学的に増強して除去する方法及び装置
US8865003B2 (en) 2008-09-26 2014-10-21 Abbott Laboratories Apparatus and method for separation of particles suspended in a liquid from the liquid in which they are suspended
SI2172211T1 (sl) 2008-10-01 2015-03-31 Immatics Biotechnologies Gmbh Sestavek s tumorjem povezanih peptidov in zadevnih cepiv proti raku za zdravljenje glioblastoma (GBM) in drugih vrst raka
EP2331230A1 (en) 2008-10-08 2011-06-15 FOSS Analytical A/S Separation of particles in liquids by use of a standing ultrasonic wave
US20100140185A1 (en) 2008-12-05 2010-06-10 John Hill Wastewater treatment
FR2941385B1 (fr) 2009-01-23 2011-04-01 Millipore Corp Procede pour fournir un circuit pour liquide biologique et circuit obtenu.
US20100206818A1 (en) 2009-02-19 2010-08-19 Chartered Semiconductor Manufacturing, Ltd. Ultrasonic filtration for cmp slurry
EP2412817B2 (en) 2009-03-27 2019-06-05 Asahi Kasei Medical Co., Ltd. Method for removing viruses in high-concentration monoclonal antibody solution
JP2010252785A (ja) 2009-03-31 2010-11-11 Kanagawa Acad Of Sci & Technol 細胞濃縮分離装置
WO2010117464A1 (en) 2009-04-09 2010-10-14 Sangamo Biosciences, Inc. Targeted integration into stem cells
US8476060B2 (en) 2009-04-13 2013-07-02 Board Of Regents, The University Of Texas System Process for separating lipids from a biomass
US20110095225A1 (en) 2009-04-20 2011-04-28 Origin Oil, Inc. Systems, apparatuses, and methods for extracting non-polar lipids from an aqueous algae slurry and lipids produced therefrom
IT1396003B1 (it) 2009-05-14 2012-11-09 Fidia Farmaceutici Ialuronidasi extracellulare da streptomyces koganeiensis
EP2436387B1 (en) 2009-05-25 2018-07-25 Celgene Corporation Pharmaceutical composition comprising crbn for use in treating a disease of the cerebral cortex
US8865452B2 (en) 2009-06-15 2014-10-21 Aurora Algae, Inc. Systems and methods for extracting lipids from wet algal biomass
US8772004B2 (en) 2009-06-25 2014-07-08 Old Dominion University Research Foundation System and method for high-voltage pulse assisted aggregation of algae
CN102482690A (zh) 2009-06-26 2012-05-30 钴技术有限公司 生物产物生产的集成系统和方法
DE102009032840B4 (de) 2009-07-13 2015-02-05 Epcos Ag SAW-Filterschaltung mit verbesserter ESD-Festigkeit
GB0914762D0 (en) 2009-08-24 2009-09-30 Univ Glasgow Fluidics apparatus and fluidics substrate
EP2470638B1 (en) 2009-08-26 2019-10-23 GE Healthcare Bio-Sciences Corp. Continuous recovery harvest bag
GB0915072D0 (en) 2009-09-01 2009-09-30 Prokyma Technologies Ltd Ultrasound & magnetic method
US9532769B2 (en) 2009-09-04 2017-01-03 The University Of North Carolina At Chapel Hill Systems, methods, and computer readable media for high frequency contrast imaging and image-guided therapeutics
DE102009046145A1 (de) 2009-10-29 2011-05-12 Robert Bosch Gmbh Ultraschallwandler zum Einsatz in einem fluiden Medium
US9588121B2 (en) 2009-11-06 2017-03-07 Go Therapeutics, Inc. Method for early detection of cancer
US20180223439A1 (en) 2009-11-16 2018-08-09 Flodesign Sonics, Inc. Particle-particle interaction using acoustic waves
US8691145B2 (en) 2009-11-16 2014-04-08 Flodesign Sonics, Inc. Ultrasound and acoustophoresis for water purification
EP3431608A3 (en) 2009-11-17 2019-02-20 E. R. Squibb & Sons, L.L.C. Method for enhanced protein production
US8663576B2 (en) 2009-11-25 2014-03-04 Hologic, Inc. Detection of intraamniotic infection
EP2507380A2 (en) 2009-12-03 2012-10-10 Bard Holding, Inc. Process and system for producing algal oil
EP2507639B1 (en) 2009-12-04 2020-04-01 Life Technologies Corporation Apparatuses, systems, methods, and computer readable media for acoustic flow cytometry
US8518681B2 (en) 2009-12-04 2013-08-27 Sound Surgical Technologies Llc Selective lysing of cells using ultrasound
US8591459B2 (en) 2009-12-21 2013-11-26 Ethicon Endo-Surgery, Inc. Use of biomarkers and therapeutic agents with surgical devices
CA2792691A1 (en) 2010-03-11 2011-09-15 Glaxosmithkline Biologicals S.A. Immunogenic composition or vaccine against gram-negative bacterial, for example, neisserial, infection or disease
US8722155B2 (en) 2010-03-12 2014-05-13 Los Alamos National Security, Llc Method to manufacture bit patterned magnetic recording media
GB201004551D0 (en) 2010-03-19 2010-05-05 Immatics Biotechnologies Gmbh NOvel immunotherapy against several tumors including gastrointestinal and gastric cancer
EP2558179B1 (en) 2010-04-12 2020-08-05 Flodesign Sonics Inc. Use of ultrasound and acoustophoresis technology for separation of particulates from a host medium
US8889388B2 (en) 2010-04-21 2014-11-18 Zhaowei Wang Acoustic device and methods thereof for separation and concentration
PT105058B (pt) 2010-04-21 2013-04-17 Hovione Farmaciencia S A Processo para processamento de partículas de ingredientes activos farmacêuticos
US8714360B2 (en) 2010-05-12 2014-05-06 Ethicon Endo-Surgery, Inc. Tissue processing device with ultrasonic tissue particle separator
JP5688451B2 (ja) 2010-06-04 2015-03-25 エンパイア テクノロジー ディベロップメント エルエルシー 音響的に駆動されるナノ粒子コンセントレータ
US8956538B2 (en) 2010-06-16 2015-02-17 Flodesign Sonics, Inc. Phononic crystal desalination system and methods of use
GB201010724D0 (en) 2010-06-25 2010-08-11 Isis Innovation Acoustic separators
US20130217113A1 (en) 2010-07-15 2013-08-22 Advanced Liquid Logic Inc. System for and methods of promoting cell lysis in droplet actuators
CA2993567C (en) 2010-07-21 2022-06-28 Sangamo Biosciences, Inc. Methods and compositions for modification of a t-cell receptor gene
US20130177500A1 (en) 2010-07-23 2013-07-11 Trustee Of Boston University Anti-despr inhibitors as therapeutics for inhibition of pathological angiogenesis and tumor cell invasiveness and for molecular imaging and targeted delivery
MX368531B (es) 2010-07-28 2019-10-07 Gliknik Inc Proteinas de fusion de fragmentos de proteinas humanas naturales para crear composiciones de fc de inmunoglobulinas multimerizadas ordenadamente.
WO2012015908A2 (en) 2010-07-30 2012-02-02 Millipore Corporation Chromatography media and method
ES2537207T3 (es) 2010-08-16 2015-06-03 Novimmune S.A. Métodos para la generación de anticuerpos multiespecíficos y multivalentes
US8679338B2 (en) 2010-08-23 2014-03-25 Flodesign Sonics, Inc. Combined acoustic micro filtration and phononic crystal membrane particle separation
US9421553B2 (en) 2010-08-23 2016-08-23 Flodesign Sonics, Inc. High-volume fast separation of multi-phase components in fluid suspensions
US9011699B2 (en) * 2010-08-23 2015-04-21 Flodesign Sonics, Inc. Ultrasonic agglomeration of microalgae
US8592204B2 (en) 2010-08-23 2013-11-26 Flodesign Sonics, Inc. Ultrasound and acoustophoresis for collection and processing of oleaginous microorganisms
CA2811364C (en) 2010-09-27 2022-01-04 Sangamo Biosciences, Inc. Methods and compositions for inhibiting viral entry into cells
US20170233798A1 (en) 2010-10-22 2017-08-17 T2 Biosystems, Inc. Nmr systems and methods for the rapid detection of analytes
CN103209749B (zh) 2010-11-26 2015-04-22 英派尔科技开发有限公司 空气净化系统和使用超声波的方法
US9662375B2 (en) 2010-11-30 2017-05-30 Orphazyme Aps Methods for increasing intracellular activity of Hsp70
US8895279B2 (en) 2010-12-02 2014-11-25 Dennis A. Burke Applications of the rotating photobioreactor
EP2675540B1 (en) 2010-12-03 2018-03-21 GE Healthcare Bio-Sciences AB System and process for biopolymer chromatography
US20120145633A1 (en) 2010-12-09 2012-06-14 General Electric Company Ultra-sound enhanced centrifugal separation of oil from oily solids in water and wastewater
WO2012081931A2 (ko) 2010-12-17 2012-06-21 Kim Sung-Chun 세포배양을 통한 세포 및 지용성물질의 생산 방법 및 장치
GB2486680A (en) 2010-12-22 2012-06-27 Morgan Electro Ceramics Ltd Ultrasonic or acoustic transducer that supports two or more frequencies
US9314751B2 (en) 2011-01-07 2016-04-19 Life Technologies Corporation Methods and apparatus for mixing and shipping fluids
US9428565B2 (en) 2011-01-31 2016-08-30 The General Hospital Corporation Treatment and bioluminescent visualization using multimodal TRAIL molecules
US9810665B2 (en) 2011-02-04 2017-11-07 Cidra Corporate Services, Inc. Sonic filter for measuring and capturing particles having a particular particle size in a fluid, mixture or process flow
WO2012154237A1 (en) 2011-02-04 2012-11-15 Cidra Corporate Services Inc. Optimizing acoustic efficiency of a sonic filter or separator
US9480375B2 (en) 2011-02-09 2016-11-01 The University Of Vermont & State Agricultural College Aeroacoustic duster
ES2680636T3 (es) 2011-02-14 2018-09-10 Revivicor Inc. Cerdos genéticamente modificados para xenotrasplante de xenoinjertos vascularizados y derivados de los mismos
CA2828303C (en) 2011-02-28 2020-04-07 Seattle Children's Research Institute Coupling endonucleases with end-processing enzymes drive high efficiency gene disruption
WO2012135177A2 (en) 2011-03-29 2012-10-04 Uab Research Foundation Methods and compositions for cytomegalovirus il-10 protein
US20140115726A1 (en) 2011-04-05 2014-04-24 Cellectis New tale-protein scaffolds and uses thereof
EP2700975B1 (en) 2011-04-20 2023-04-05 Nissan Motor Co., Ltd. Mounting structure for sonar sensor
US8668886B2 (en) 2011-04-24 2014-03-11 Therapeutic Proteins International, LLC Separative bioreactor
GB2528401A (en) 2011-05-06 2016-01-20 Nvip Pty Ltd Anti-nerve growth factor antibodies and methods of preparing and using the same
GB201114858D0 (en) 2011-08-29 2011-10-12 Nvip Pty Ltd Anti-nerve growth factor antibodies and methods of using the same
CA2836460C (en) 2011-05-17 2021-09-21 Natrix Separations Inc. Methods of using a fluid treatment device
EP3363812A1 (en) 2011-06-23 2018-08-22 Ablynx NV Techniques for predicting, detecting and reducing aspecific protein interference in assays involving immunoglobin single variable domains
CN108659121A (zh) 2011-06-23 2018-10-16 埃博灵克斯股份有限公司 用于预测、检测和减少涉及免疫球蛋白单可变结构域的测定法中的非特异性蛋白干扰的技术
JP2014520530A (ja) 2011-06-27 2014-08-25 フリアー システムズ， インコーポレイテッド 混合性核酸試料からの標的核酸の隔離
DE102011107533A1 (de) 2011-07-14 2013-01-17 Airbus Operations Gmbh Tür für ein Aufenthaltsmodul, Aufenthaltsmodul und Luftfahrzeug
FR2979256B1 (fr) 2011-08-30 2014-09-26 Centre Nat Rech Scient Dispositif de manipulation d'objets par champs de force acoustique
US8932586B2 (en) 2011-09-06 2015-01-13 Intrexon Corporation Modified forms of Pseudomonas exotoxin A
NL1039052C2 (en) 2011-09-19 2013-03-21 Stichting Wetsus Ct Excellence Sustainable Water Technology Device and method for a heterogeneous waste water treatment reactor.
NL1039053C2 (en) 2011-09-19 2013-03-21 Stichting Wetsus Ct Excellence Sustainable Water Technology Device and method for a bioreactor, catalysis reactor or crystallizer without internals.
EP2761291B1 (en) 2011-09-28 2016-06-29 Acousort AB System and method to separate cells and/or particles
US9376655B2 (en) 2011-09-29 2016-06-28 Life Technologies Corporation Filter systems for separating microcarriers from cell culture solutions
US9175535B2 (en) 2011-09-29 2015-11-03 Coil Solutions, Inc. Propulsion generator and method
WO2013049623A1 (en) 2011-09-30 2013-04-04 Brian David Warner Fluid exchange methods and devices
ES2673957T5 (es) 2011-10-03 2021-12-15 Croda Int Plc Nanopartículas, procedimiento para la preparación y utilización de las mismas como portadoras de moléculas anfipáticas o hidrofóbicas en el campo de la medicina, incluyendo el tratamiento del cáncer, y compuestos de tipo alimentario
JP6435193B2 (ja) 2011-10-19 2018-12-05 ノビミューン エスアー 抗体を精製する方法
FR2982082B1 (fr) 2011-11-02 2013-11-22 Fabien Gaben Procede de fabrication de batteries en couches minces entierement solides
FR2982083B1 (fr) 2011-11-02 2014-06-27 Fabien Gaben Procede de realisation de films minces d'electrolyte solide pour les batteries a ions de lithium
GB201119192D0 (en) 2011-11-07 2011-12-21 Ucl Business Plc Chromatography medium
JP6057251B2 (ja) 2011-11-11 2017-01-11 国立研究開発法人産業技術総合研究所 粒子分別装置および粒子分別方法
US8256076B1 (en) 2011-11-19 2012-09-04 Murray F Feller Method of making an ultrasonic transducer
GB201120143D0 (en) 2011-11-22 2012-01-04 Micromass Ltd Droplet manipulation using gas-phase standing-wave ultrasound fields in MS sources
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method
WO2013078455A2 (en) 2011-11-23 2013-05-30 The Board Of Regents Of The University Of Texas System Proteomic identification of antibodies
EP2785743B1 (en) 2011-12-01 2019-08-14 The Brigham and Women's Hospital, Inc. Anti-ceacam1 recombinant antibodies for cancer therapy
GB201120887D0 (en) 2011-12-06 2012-01-18 The Technology Partnership Plc Acoustic sensor
WO2013091657A1 (en) 2011-12-22 2013-06-27 Herlev Hospital Therapeutic applications of calcium electroporation to effectively induce tumor necrosis
JP2015512766A (ja) 2012-01-31 2015-04-30 ザ・ペン・ステート・リサーチ・ファンデーション 可変定常的表面弾性波を用いたマイクロ流体操作及び粒子の分類
WO2013119880A1 (en) 2012-02-07 2013-08-15 Global Bio Therapeutics Usa, Inc. Compartmentalized method of nucleic acid delivery and compositions and uses thereof
GB2499251A (en) 2012-02-13 2013-08-14 Univ United Arab Emirates Treatment of industrial and petroleum refinery wastewater
ES2659941T3 (es) 2012-02-27 2018-03-20 Centre National De La Recherche Scientifique Método de formación de un agregado de células
CA2862756A1 (en) 2012-02-29 2013-09-06 Kci Licensing, Inc. Systems and methods for supplying reduced pressure and measuring flow using a disc pump system
CN104066990B (zh) 2012-03-07 2017-02-22 凯希特许有限公司 带有高级致动器的盘泵
WO2013138505A1 (en) 2012-03-13 2013-09-19 Salk Institute For Biological Studies Selective cell targeting using adenovirus and chemical dimers
AU2013231972B2 (en) 2012-03-14 2018-03-22 Salk Institute For Biological Studies Adenoviral tumor diagnostics
US10953436B2 (en) 2012-03-15 2021-03-23 Flodesign Sonics, Inc. Acoustophoretic device with piezoelectric transducer array
US9567559B2 (en) 2012-03-15 2017-02-14 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US9422328B2 (en) 2012-03-15 2016-08-23 Flodesign Sonics, Inc. Acoustic bioreactor processes
US9822333B2 (en) 2012-03-15 2017-11-21 Flodesign Sonics, Inc. Acoustic perfusion devices
US9416344B2 (en) 2012-03-15 2016-08-16 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US9796956B2 (en) 2013-11-06 2017-10-24 Flodesign Sonics, Inc. Multi-stage acoustophoresis device
US9340435B2 (en) 2012-03-15 2016-05-17 Flodesign Sonics, Inc. Separation of multi-component fluid through ultrasonic acoustophoresis
US10322949B2 (en) 2012-03-15 2019-06-18 Flodesign Sonics, Inc. Transducer and reflector configurations for an acoustophoretic device
US9745548B2 (en) 2012-03-15 2017-08-29 Flodesign Sonics, Inc. Acoustic perfusion devices
US10040011B2 (en) 2012-03-15 2018-08-07 Flodesign Sonics, Inc. Acoustophoretic multi-component separation technology platform
US9738866B2 (en) 2012-03-15 2017-08-22 Flodesign Sonics, Inc. Acoustic perfusion devices
US9783775B2 (en) 2012-03-15 2017-10-10 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US9623348B2 (en) 2012-03-15 2017-04-18 Flodesign Sonics, Inc. Reflector for an acoustophoretic device
US9752113B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc. Acoustic perfusion devices
US9272234B2 (en) 2012-03-15 2016-03-01 Flodesign Sonics, Inc. Separation of multi-component fluid through ultrasonic acoustophoresis
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
US9752114B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc Bioreactor using acoustic standing waves
US9688958B2 (en) 2012-03-15 2017-06-27 Flodesign Sonics, Inc. Acoustic bioreactor processes
WO2013138797A1 (en) 2012-03-15 2013-09-19 Flodesign Sonics, Inc. Acoustophoretic multi-component separation technology platform
US20170191022A1 (en) 2012-03-15 2017-07-06 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US9797897B2 (en) 2012-03-26 2017-10-24 Duke University Acoustically responsive particles
PT106237B (pt) 2012-03-30 2015-03-19 Hovione Farmaci Ncia S A Produção de partículas substancialmente monodispersas utilizando moagem e separação por membranas
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9878056B2 (en) 2012-04-02 2018-01-30 Modernatx, Inc. Modified polynucleotides for the production of cosmetic proteins and peptides
EP2833892A4 (en) 2012-04-02 2016-07-20 Moderna Therapeutics Inc MODIFIED POLYNUCLEOTIDES FOR THE PRODUCTION OF PROTEINS AND PEPTIDES ASSOCIATED WITH ONCOLOGY
RU2017113508A (ru) 2012-04-20 2019-01-28 Флоудизайн Соникс Инк. Акустофоретическая сепарация липидных частиц от эритроцитов
US11324873B2 (en) 2012-04-20 2022-05-10 Flodesign Sonics, Inc. Acoustic blood separation processes and devices
US9357293B2 (en) 2012-05-16 2016-05-31 Siemens Aktiengesellschaft Methods and systems for Doppler recognition aided method (DREAM) for source localization and separation
US9517474B2 (en) 2012-05-18 2016-12-13 University Of Georgia Research Foundation, Inc. Devices and methods for separating particles
KR102247979B1 (ko) 2012-05-25 2021-05-04 셀렉티스 면역요법을 위한 동종이형 및 면역억제제 저항성인 ｔ 세포의 조작 방법
JP2015519537A (ja) 2012-06-14 2015-07-09 ベリアブスキィ，ヤン エネルギー移動方法および装置
US9820768B2 (en) 2012-06-29 2017-11-21 Ethicon Llc Ultrasonic surgical instruments with control mechanisms
US9689802B2 (en) 2012-06-29 2017-06-27 Southwest Research Institute Systems, methods and apparatus for analysis of multiphase fluid mixture in pipelines
US9657290B2 (en) 2012-07-03 2017-05-23 The Board Of Trustees Of The Leland Stanford Junior University Scalable bio-element analysis
US8709250B2 (en) 2012-07-12 2014-04-29 Heliae Development, Llc Tubular electro-acoustic aggregation device
EP2872234A1 (en) 2012-07-16 2015-05-20 Flodesign Sonics Inc. Improved separation of multi-component fluid through ultrasonic acoustophoresis
EP3494997B1 (en) 2012-07-25 2019-09-18 The Broad Institute, Inc. Inducible dna binding proteins and genome perturbation tools and applications thereof
WO2014022268A1 (en) 2012-08-01 2014-02-06 The Penn State Research Foundation High efficiency separation and manipulation of particles and cells
WO2014029505A1 (en) 2012-08-22 2014-02-27 Eth Zurich Acoustophoretic contactless transport and handling of matter in air
AU2013309488A1 (en) 2012-08-29 2015-03-05 Nature Technology Corporation DNA plasmids with improved expression
US9605074B2 (en) 2012-08-30 2017-03-28 The General Hospital Corporation Multifunctional nanobodies for treating cancer
EP2897709B1 (en) 2012-09-21 2019-10-30 AcouSort AB A method for separating cells-bead complexes
JP2014079748A (ja) 2012-09-26 2014-05-08 Hitachi Ltd 超音波を用いた懸濁液処理装置
CA2887318C (en) 2012-10-02 2021-07-27 Bart Lipkens Acoustophoretic separation technology using multi-dimensional standing waves
EP2906684B8 (en) 2012-10-10 2020-09-02 Sangamo Therapeutics, Inc. T cell modifying compounds and uses thereof
US9480991B2 (en) 2012-10-12 2016-11-01 Elwha Llc Radiofrequency particle separator
US9095367B2 (en) 2012-10-22 2015-08-04 Ethicon Endo-Surgery, Inc. Flexible harmonic waveguides/blades for surgical instruments
US10201365B2 (en) 2012-10-22 2019-02-12 Ethicon Llc Surgeon feedback sensing and display methods
WO2014066553A1 (en) 2012-10-26 2014-05-01 Becton, Dickinson And Company Devices and methods for manipulating components in a fluid sample
DE102012022146A1 (de) 2012-11-12 2014-05-15 Physik Instrumente (Pi) Gmbh & Co. Kg Ultraschallaktor für einen linearen Ultraschallmotor sowie linearer Ultraschallmotor mit einem Ultraschallaktor
US9080167B2 (en) 2012-11-16 2015-07-14 Covaris, Inc. System and method for processing paraffin embedded samples
US8900532B2 (en) 2012-11-16 2014-12-02 The Charles Stark Draper Laboratory, Inc. Apparatus and method for separating plasma from blood and delayed wetting
WO2014077863A1 (en) 2012-11-19 2014-05-22 Nature Technology Corporation Replicative minicircle vectors with improved expression
US9938390B2 (en) 2012-11-26 2018-04-10 Eth Zurich Method for the preparation of macroporous particles and macroporous microclusters
JP6144355B2 (ja) 2012-11-26 2017-06-07 モデルナティエックス インコーポレイテッドＭｏｄｅｒｎａＴＸ，Ｉｎｃ． 化学修飾ｍＲＮＡ
PL2735360T3 (pl) 2012-11-26 2017-09-29 Gambro Lundia Ab Urządzenie adsorbujące łączące granulki i membrany z włókien kanalikowych
FR2998891B1 (fr) 2012-11-30 2015-04-10 Commissariat Energie Atomique Procede pour preparer un verre a porosite bimodale, eventuellement fonctionnalise et ledit verre
EP2928303A4 (en) 2012-12-07 2016-07-13 Haplomics Inc FACTOR VIII MUTATION REPAIR AND TOLERANCE INDUCTION
EP2931892B1 (en) 2012-12-12 2018-09-12 The Broad Institute, Inc. Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
MX2015007550A (es) 2012-12-12 2017-02-02 Broad Inst Inc Suministro, modificación y optimización de sistemas, métodos y composiciones para la manipulación de secuencias y aplicaciones terapéuticas.
US20140186843A1 (en) 2012-12-12 2014-07-03 Massachusetts Institute Of Technology Methods, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
JP2016504050A (ja) 2013-01-17 2016-02-12 モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． 細胞表現型の改変のためのシグナルセンサーポリヌクレオチド
US9504780B2 (en) 2013-01-30 2016-11-29 The Charles Stark Draper Laboratory, Inc. Extracorporeal clearance of organophosphates from blood on an acoustic separation device
CN107189933B (zh) 2013-02-07 2021-06-18 弗洛设计声能学公司 利用声驻波的生物反应器系统和相关方法
WO2014126548A1 (en) 2013-02-12 2014-08-21 Faurecia Emissions Control Technologies Vehicle exhaust system with resonance damping
CN105164535B (zh) 2013-02-14 2018-04-10 法龙药品公司 测定急性呼吸窘迫综合征(ards)相关生物标志物的方法、监测患者的ards的发展和治疗的方法
US9554794B2 (en) 2013-03-01 2017-01-31 Ethicon Endo-Surgery, Llc Multiple processor motor control for modular surgical instruments
BR112015021098B1 (pt) 2013-03-01 2022-02-15 Ethicon Endo-Surgery, Inc Cobertura para uma junta de articulação e instrumento cirúrgico
WO2014138203A2 (en) 2013-03-05 2014-09-12 Board Of Regents, The University Of Texas System Microfluidic devices for the rapid and automated processing of sample populations
WO2014138739A1 (en) 2013-03-08 2014-09-12 The Charles Stark Draper Laboratory, Inc. System and method for blood separation by microfluidic acoustic focusing
EP2970886B1 (en) 2013-03-12 2018-05-23 Sangamo Therapeutics, Inc. Methods and compositions for modification of hla
US9725709B2 (en) 2013-03-12 2017-08-08 OpenCell Technologies, Inc. Intracellular delivery and transfection methods and devices
WO2014152211A1 (en) 2013-03-14 2014-09-25 Moderna Therapeutics, Inc. Formulation and delivery of modified nucleoside, nucleotide, and nucleic acid compositions
US9388363B2 (en) 2013-03-15 2016-07-12 Megasonic Sweeping, Incorporated Ultrasonic and megasonic method for extracting palm oil
EP2975942B1 (en) 2013-03-21 2018-08-08 Sangamo Therapeutics, Inc. Targeted disruption of t cell receptor genes using engineered zinc finger protein nucleases
AU2014245806B2 (en) 2013-03-28 2019-10-31 The University Of British Columbia Microfluidic devices and methods for use thereof in multicellular assays of secretion
AR095962A1 (es) 2013-04-01 2015-11-25 Moreinx Ab Nanopartículas, compuestas de esterol y saponina de quillaja saponaria molina, proceso para preparación y uso de las mismas como portadores para moléculas anfipáticas o hidrófobas en el campo de la medicina incluyendo tratamiento de cáncer y compuestos relacionados con alimentos
SG11201508804QA (en) 2013-04-25 2015-11-27 Flodesign Sonics Inc Excipient removal from pharmacological samples
JP6463734B2 (ja) 2013-05-06 2019-02-06 サノフイＳａｎｏｆｉ 抗体を精製するための連続的多工程法
RS61778B1 (sr) 2013-05-06 2021-06-30 Scholar Rock Inc Kompozicije i postupci za modulaciju faktora rasta
US9629877B2 (en) 2013-05-14 2017-04-25 Board Of Regents, The University Of Texas System Human application of engineered chimeric antigen receptor (CAR) T-cells
CN116083487A (zh) 2013-05-15 2023-05-09 桑格摩生物治疗股份有限公司 用于治疗遗传病状的方法和组合物
ES2883131T3 (es) 2013-05-29 2021-12-07 Cellectis Métodos para la modificación de células T para inmunoterapia utilizando el sistema de nucleasa CAS guiado por ARN
CA2913872C (en) 2013-05-31 2022-01-18 Cellectis A laglidadg homing endonuclease cleaving the t-cell receptor alpha gene and uses thereof
JP6488283B2 (ja) 2013-05-31 2019-03-20 セレクティスＣｅｌｌｅｃｔｉｓ Ｃ−ｃケモカイン受容体５型（ｃｃｒ５）遺伝子を開裂するｌａｇｌｉｄａｄｇホーミングエンドヌクレアーゼおよびその用途
KR101442486B1 (ko) 2013-06-07 2014-09-24 아이에스테크놀로지 주식회사 초음파를 이용한 유체내 불순물 분리장치 및 분리방법
WO2014204723A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Oncogenic models based on delivery and use of the crispr-cas systems, vectors and compositions
US9725690B2 (en) 2013-06-24 2017-08-08 Flodesign Sonics, Inc. Fluid dynamic sonic separator
WO2015003012A2 (en) 2013-07-01 2015-01-08 Olivier Berteau Distributed perfusion bioreactor system for continuous culture of biological cells
CA2918036C (en) 2013-07-12 2018-04-24 Thomas J. Kennedy, Iii Acoustic bioreactor processes
TWI777198B (zh) 2013-08-05 2022-09-11 德商伊瑪提克斯生物科技有限公司 新穎肽類，細胞及其用於治療多種腫瘤的用途，其製造方法及包含其等之醫藥組成物（七）
ES2900004T3 (es) 2013-08-05 2022-03-15 Immatics Biotechnologies Gmbh Nueva inmunoterapia contra diversos tumores como el cáncer de pulmón, incluido el carcinoma de pulmón amicrocítico (NSCLC)
CA2920899C (en) 2013-08-28 2023-02-28 Sangamo Biosciences, Inc. Compositions for linking dna-binding domains and cleavage domains
US9745569B2 (en) 2013-09-13 2017-08-29 Flodesign Sonics, Inc. System for generating high concentration factors for low cell density suspensions
JP6292808B2 (ja) 2013-09-13 2018-03-14 デクセリアルズ株式会社 接着剤、及び発光装置
EP3043826A4 (en) 2013-09-13 2017-05-24 Moderna Therapeutics, Inc. Polynucleotide compositions containing amino acids
WO2015048744A2 (en) 2013-09-30 2015-04-02 Moderna Therapeutics, Inc. Polynucleotides encoding immune modulating polypeptides
US10323076B2 (en) 2013-10-03 2019-06-18 Modernatx, Inc. Polynucleotides encoding low density lipoprotein receptor
CN105899665B (zh) 2013-10-17 2019-10-22 桑格摩生物科学股份有限公司 用于核酸酶介导的基因组工程改造的递送方法和组合物
EP3058082A4 (en) 2013-10-18 2017-04-26 ModernaTX, Inc. Compositions and methods for tolerizing cellular systems
US10076574B2 (en) 2013-10-25 2018-09-18 Wayne State University Methods, systems and compositions relating to cell conversion via protein-induced in-vivo cell reprogramming
GB201319446D0 (en) 2013-11-04 2013-12-18 Immatics Biotechnologies Gmbh Personalized immunotherapy against several neuronal and brain tumors
EP3065795A1 (en) 2013-11-05 2016-09-14 Flodesign Sonics Inc. Acoustophoresis device with modular components
AU2014346559B2 (en) 2013-11-07 2020-07-09 Editas Medicine,Inc. CRISPR-related methods and compositions with governing gRNAs
JP6530393B2 (ja) 2013-11-12 2019-06-12 ヴリジェ ユニヴェルシテ ブリュッセル Ｒｎａ転写ベクター及びその使用
FR3012972A1 (fr) 2013-11-14 2015-05-15 Biomerieux Sa Nouveau milieu filtrant pour l'obtention de plasma, dispositif et procede de filtration associes
CA2930665A1 (en) 2013-11-18 2015-05-21 Rubius Therapeutics, Inc. Synthetic membrane-receiver complexes
DE102013224569B3 (de) 2013-11-29 2014-06-26 Physik Instrumente (Pi) Gmbh & Co. Kg Ultraschallmotor und Verfahren zum Betreiben eines solchen Ultraschallmotors
US20170173128A1 (en) 2013-12-06 2017-06-22 Moderna TX, Inc. Targeted adaptive vaccines
CN106536729A (zh) 2013-12-12 2017-03-22 布罗德研究所有限公司 使用粒子递送组分靶向障碍和疾病的crispr‑cas系统和组合物的递送、用途和治疗应用
CN105899657A (zh) 2013-12-12 2016-08-24 布罗德研究所有限公司 用于改变基因产物表达的crispr-cas系统和方法、结构信息以及诱导型模块化cas酶
GB201322103D0 (en) 2013-12-13 2014-01-29 The Technology Partnership Plc Fluid pump
DE102013226418B3 (de) 2013-12-18 2015-04-02 Physik Instrumente (Pi) Gmbh & Co. Kg Ultraschallmotor
EP3083691A2 (en) 2013-12-20 2016-10-26 Cellectis Method of engineering multi-input signal sensitive t cell for immunotherapy
US20160312168A1 (en) 2013-12-30 2016-10-27 Ge Healthcare Bio-Sciences Corp. Apparatus for cell cultivation
US20170002060A1 (en) 2014-01-08 2017-01-05 Moderna Therapeutics, Inc. Polynucleotides for the in vivo production of antibodies
US20160332159A1 (en) 2014-01-15 2016-11-17 Eth Zurich Acoustophoretic droplet handling in bulk acoustic wave devices
US9878536B2 (en) 2014-01-24 2018-01-30 President And Fellows Of Harvard College Acoustophoretic printing apparatus and method
EP3107552B1 (en) 2014-02-21 2018-03-28 Cellectis Method for in situ inhibition of regulatory t cells
CN106414702A (zh) 2014-03-10 2017-02-15 弗洛设计声能学公司 具有声致泳动设备的一次性生物反应器
KR102228828B1 (ko) 2014-03-11 2021-03-16 셀렉티스 동종이형 이식에 양립성인 t-세포들을 만들어내는 방법
US8820538B1 (en) 2014-03-17 2014-09-02 Namocell LLC Method and apparatus for particle sorting
DK3569619T3 (da) 2014-03-19 2021-06-14 Cellectis Fremgangsmåde til fremstilling af CD123-specifikke CAR T-celler til cancerimmunterapi
WO2015143335A1 (en) 2014-03-20 2015-09-24 The University Of North Carolina At Chapel Hill Methods and compositions for chimeric coronavirus spike proteins
EP3129484A1 (en) 2014-03-25 2017-02-15 Editas Medicine, Inc. Crispr/cas-related methods and compositions for treating hiv infection and aids
DE102014205577B4 (de) 2014-03-26 2021-05-12 Physik Instrumente (Pi) Gmbh & Co. Kg Ultraschallmotor
WO2015148842A1 (en) 2014-03-28 2015-10-01 The Regents Of The University Of California Efficient delivery of large cargos into cells on a porous substrate
US9982265B2 (en) 2014-03-28 2018-05-29 Board Of Regents, The University Of Texas System Inhibition of Bruton's tyrosine kinase (Btk) in the lung to treat severe lung inflammation and lung injury
US10047650B2 (en) 2014-03-31 2018-08-14 Faurencia Emissions Technologies USA, LLC Vehicle exhaust system with resistive patch
CA2944492A1 (en) 2014-04-01 2015-10-08 Rubius Therapeutics, Inc. Methods and compositions for immunomodulation
WO2015153789A1 (en) 2014-04-01 2015-10-08 Editas Medicine, Inc. Crispr/cas-related methods and compositions for treating herpes simplex virus type 1 (hsv-1)
DK3126390T3 (da) 2014-04-03 2020-01-20 Cellectis Cd33-specifikke kimære antigenreceptorer til cancerimmunterapi
DE102014206823A1 (de) 2014-04-09 2015-10-15 Siemens Aktiengesellschaft Vorrichtung zum Trennen einer Emulsion und/oder einer Suspension
US10765728B2 (en) 2014-04-11 2020-09-08 Cellectis Method for generating immune cells resistant to arginine and/or tryptophan depleted microenvironment
JP6698546B2 (ja) 2014-04-14 2020-05-27 セレクティスＣｅｌｌｅｃｔｉｓ 癌免疫療法のためのｂｃｍａ（ｃｄ２６９）特異的キメラ抗原受容体
CN106459924A (zh) 2014-04-23 2017-02-22 得克萨斯州大学系统董事会 用于疗法中的嵌合抗原受体(car)及其制备方法
BR112016024644A2 (pt) 2014-04-23 2017-10-10 Modernatx Inc vacinas de ácido nucleico
WO2015164829A1 (en) 2014-04-24 2015-10-29 New Jersey Institute Of Technology Concealed fastener window or curtain wall assemblies
WO2015171691A2 (en) 2014-05-06 2015-11-12 Scholar Rock, Inc. Compositions and methods for growth factor modulation
EP3140387A1 (en) 2014-05-08 2017-03-15 Flodesign Sonics Inc. Acoustophoretic device with piezoelectric transducer array
CN106459914B (zh) 2014-05-15 2020-11-06 新加坡国立大学 经修饰的自然杀伤细胞及其用途
CN106536059B (zh) 2014-06-09 2019-01-11 阿森特生物纳米科技股份有限公司 用于颗粒的控制和分拣系统
GB201410262D0 (en) 2014-06-10 2014-07-23 Cambridge Entpr Ltd Novel method
WO2015195453A2 (en) 2014-06-16 2015-12-23 Emd Millipore Corporation Methods for increasing the capacity of flow-through processes
US10000533B2 (en) 2014-06-20 2018-06-19 Immatics Biotechnologies Gmbh Immunotherapy against several tumors of the blood, in particular chronic lymphoid leukemia (CLL)
US9744483B2 (en) 2014-07-02 2017-08-29 Flodesign Sonics, Inc. Large scale acoustic separation device
WO2016004398A2 (en) 2014-07-02 2016-01-07 Flodesign Sonics, Inc. Acoustophoretic device with uniform fluid flow
ES2726671T3 (es) 2014-07-14 2019-10-08 Westinghouse Electric Co Llc Conjunto de medición termoacústica de la distribución de potencia nuclear
US9605266B2 (en) 2014-07-16 2017-03-28 City Of Hope Cell-specific internalizing RNA aptamers against human CCR5 and uses therefore
US9908288B2 (en) 2014-07-29 2018-03-06 The Boeing Company Free-form spatial 3-D printing using part levitation
AU2015295349B2 (en) 2014-07-29 2020-09-24 Cellectis ROR1(NTRKR1)specific chimeric antigen receptors for cancer immunotherapy
WO2016019144A2 (en) 2014-07-30 2016-02-04 Sangamo Biosciences, Inc. Gene correction of scid-related genes in hematopoietic stem and progenitor cells
WO2016022851A1 (en) 2014-08-06 2016-02-11 Children's Medical Center Corporation Modified integrin polypeptides, modified integrin polypeptide dimers, and uses thereof
US20170232439A1 (en) 2014-08-11 2017-08-17 Carnegie Mellon University Separation of low-abundance cells from fluid using surface acoustic waves
WO2016028682A1 (en) 2014-08-17 2016-02-25 The Broad Institute Inc. Genome editing using cas9 nickases
HUE043847T2 (hu) 2014-08-28 2019-09-30 Halozyme Inc Hialuronán-lebontó enzimmel és egy immun checkpoint inhibitorral végzett kombinációs terápia
US20160060615A1 (en) 2014-09-03 2016-03-03 Thomas Walther Device for the identification, separation and / or cell type-specific manipulation of at least one cell of a cellular system
US20160082129A1 (en) 2014-09-24 2016-03-24 Aerpio Therapeutics, Inc. VE-PTP Extracellular Domain Antibodies Delivered by a Gene Therapy Vector
WO2016049251A1 (en) 2014-09-24 2016-03-31 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for modeling mutations in leukocytes
WO2016049258A2 (en) 2014-09-25 2016-03-31 The Broad Institute Inc. Functional screening with optimized functional crispr-cas systems
US9675906B2 (en) 2014-09-30 2017-06-13 Flodesign Sonics, Inc. Acoustophoretic clarification of particle-laden non-flowing fluids
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
US10238741B2 (en) 2014-10-10 2019-03-26 The Trustees Of Columbia University In The City Of New York Nucleic acid constructs for presentation of CD4 and CD8 epitopes, cellular transfection and uses thereof
EA201700181A1 (ru) 2014-10-14 2017-09-29 Галозим, Инк. Композиции аденозиндеаминазы-2 (ада-2), их варианты и способы использования
CN107110765B (zh) 2014-11-03 2020-10-02 通用医疗公司 在微流体装置中浓缩颗粒
US9879087B2 (en) 2014-11-12 2018-01-30 Siamab Therapeutics, Inc. Glycan-interacting compounds and methods of use
US20160153249A1 (en) 2014-12-02 2016-06-02 Chevron U.S.A. Inc. Systems and Methods for Reducing Pipeline Erosion Using Acoustic Radiation
DE102014225154B3 (de) 2014-12-08 2016-03-03 Physik Instrumente (Pi) Gmbh & Co. Kg Verfahren und Vorrichtung zur Ansteuerung eines piezoelektrischen Motors
US10098993B2 (en) 2014-12-10 2018-10-16 Medtronic, Inc. Sensing and storage system for fluid balance
AU2015360502A1 (en) 2014-12-10 2017-06-29 Regents Of The University Of Minnesota Genetically modified cells, tissues, and organs for treating disease
DK3035519T3 (en) 2014-12-19 2018-06-06 Attocube Systems Ag Electromechanical actuator
GB201501017D0 (en) 2014-12-23 2015-03-04 Immatics Biotechnologies Gmbh Novel peptides and combination of peptides for use in immunotherapy against hepatocellular carcinoma (HCC) and other cancers
EP3245001B1 (en) 2015-01-12 2024-06-12 Instrumentation Laboratory Company Spatial separation of particles in a particle containing solution for biomedical sensing and detection
JP6901404B2 (ja) 2015-01-21 2021-07-14 ユタ バレー ユニバーシティ 空間的な収束、操作、およびパターニングのための定在波場の高調波変調用システムおよび方法
EP3250605A1 (en) 2015-01-26 2017-12-06 Cellectis Anti-hsp70 specific chimeric antigen receptors (cars) for cancer immunotherapy
DE102015101542A1 (de) 2015-02-03 2016-08-18 ACO Severin Ahlmann GmbH & Co Kommanditgesellschaft Verfahren und Vorrichtung zur Abtrennung von Stoffen
CN107635581A (zh) 2015-02-13 2018-01-26 赛诺菲 用于单克隆抗体的稳定液体制剂
US20160237110A1 (en) 2015-02-16 2016-08-18 Flodesign Sonics, Inc. Acoustic microreactor and methods of use thereof
US20180246103A1 (en) 2015-02-16 2018-08-30 Flodesign Sonics, Inc. Acoustic microreactor and methods of use thereof
WO2016134115A1 (en) 2015-02-20 2016-08-25 Trustees Of Boston University Theranostic compositions and uses thereof
US10324082B2 (en) 2015-03-03 2019-06-18 Quest Diagnostics Investments Llc Methods for quantitation of insulin levels by mass spectrometry
AR103852A1 (es) 2015-03-06 2017-06-07 Genentech Inc DsbA Y DsbC ULTRAPURIFICADOS Y SUS MÉTODOS DE PREPARACIÓN Y USO
NL2014433B1 (nl) 2015-03-10 2016-10-13 Exsilent Res Bv Persoonlijke gehoorinrichting, in het bijzonder een hoortoestel.
US10570382B2 (en) 2015-03-11 2020-02-25 Board Of Regents, The University Of Texas System Transposase polypeptides and uses thereof
US10106770B2 (en) 2015-03-24 2018-10-23 Flodesign Sonics, Inc. Methods and apparatus for particle aggregation using acoustic standing waves
WO2016154475A1 (en) 2015-03-24 2016-09-29 Flodesign Sonics, Inc. Methods and apparatus for particle aggregation using acoustic standing waves
WO2016154578A1 (en) 2015-03-25 2016-09-29 Eta Diagnostics, Inc. An optical cell constructed by anodically bonding a thin metal layer between two optically clear glass windows
WO2016154596A1 (en) 2015-03-25 2016-09-29 Editas Medicine, Inc. Crispr/cas-related methods, compositions and components
CN104722106B (zh) 2015-03-25 2016-04-06 陕西师范大学 一种细微颗粒的超声分离装置
WO2016154579A2 (en) 2015-03-26 2016-09-29 Editas Medicine, Inc. Crispr/cas-mediated gene conversion
US20180071981A1 (en) 2015-03-31 2018-03-15 The Regents Of The University Of California System and method for tunable patterning and assembly of particles via acoustophoresis
US9855382B2 (en) 2015-03-31 2018-01-02 Biomet Biologics, Llc Cell washing device using standing acoustic waves and a phantom material
US10737012B2 (en) 2015-03-31 2020-08-11 Biomet Biologics, Inc. Cell washing using acoustic waves
US10794865B2 (en) 2015-04-01 2020-10-06 Triad National Security, Llc Ultrasonic in-situ water-cut measurement using ultrasonic oil-water separation for affecting sound speed calibration
US20180095067A1 (en) 2015-04-03 2018-04-05 Abbott Laboratories Devices and methods for sample analysis
US20180066223A1 (en) 2015-04-17 2018-03-08 Xcell Biosciences, Inc. Cancer cell enrichment system
CA2982365A1 (en) 2015-04-17 2016-10-20 Xcell Biosciences, Inc. Cancer cell enrichment system
US10900902B2 (en) 2015-04-20 2021-01-26 Unm Rainforest Innovations Method for simultaneous spectrally resolved detection or imaging of items in multiple flowing streams
US9947431B2 (en) 2015-04-21 2018-04-17 The Florida International University Board Of Trustees Anisotropic films templated using ultrasonic focusing
CA2983293C (en) 2015-04-24 2023-06-20 The Regents Of The University Of California Modulators of ror1-ror2 binding
US10640760B2 (en) 2016-05-03 2020-05-05 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
CA2984492A1 (en) 2015-04-29 2016-11-03 Flodesign Sonics, Inc. Acoustophoretic device for angled wave particle deflection
WO2016176611A2 (en) 2015-04-30 2016-11-03 Wilsa Holding, Llc Method and apparatus for conditioning fluids
PT3289362T (pt) 2015-04-30 2022-06-21 European Molecular Biology Laboratory Deteção e classificação de gotas microfluídicas
WO2016178167A1 (en) 2015-05-04 2016-11-10 Vcn Biosciences Sl Oncolytic adenoviruses with mutations in immunodominant adenovirus epitopes and their use in cancer treatment
WO2016179564A1 (en) 2015-05-06 2016-11-10 Flodesign Sonics, Inc. Acoustic pre-conditioner
US10334390B2 (en) 2015-05-06 2019-06-25 Idan BAKISH Method and system for acoustic source enhancement using acoustic sensor array
CN107708761B (zh) 2015-05-07 2022-04-29 艾尼蒂斯科技公司 使用相应方法分离血液的封闭式一次性无菌多血袋系统
WO2017191289A1 (en) 2016-05-04 2017-11-09 Aenitis Technologies Multiple blood bag system
US10414810B2 (en) 2015-05-07 2019-09-17 H. Lee Moffitt Cancer Center And Research Institute, Inc. Double mutant survivin vaccine
EP3015542A1 (de) 2015-05-07 2016-05-04 Bayer Technology Services GmbH Modulare anlage und verfahren zur kontinuierlichen, keimreduzierten produktion und/oder aufbereitung eines produktes
EP3294888A1 (en) 2015-05-11 2018-03-21 Editas Medicine, Inc. Crispr/cas-related methods and compositions for treating hiv infection and aids
WO2016182959A1 (en) 2015-05-11 2016-11-17 Editas Medicine, Inc. Optimized crispr/cas9 systems and methods for gene editing in stem cells
WO2016180918A1 (en) 2015-05-12 2016-11-17 Platod Combination of pharmacological and microfluidic features for improved platelets production
AU2016262093B2 (en) 2015-05-13 2022-08-18 Seattle Children' S Hospital (Dba Seattle Children 's Research Institute) Enhancing endonuclease based gene editing in primary cells
EP3037513A1 (de) 2015-05-13 2016-06-29 Bayer Technology Services GmbH Verfahren zur kontinuierlichen elution eines produktes von chromatographiesäulen
PT3294764T (pt) 2015-05-15 2021-02-15 Hope City Composições de recetores de antigénios quiméricos
CN104892672B (zh) 2015-05-15 2018-09-21 浙江九洲药业股份有限公司 手性螺环膦-氮-硫三齿配体及其制备方法和应用
EP3298046A1 (en) 2015-05-20 2018-03-28 Cellectis Anti-gd3 specific chimeric antigen receptors for cancer immunotherapy
KR102603273B1 (ko) 2015-05-20 2023-11-16 프로디자인 소닉스, 인크. 정재파장에서 입자의 음향 조작
US9686096B2 (en) 2015-05-20 2017-06-20 Flodesign Sonics, Inc. Acoustic manipulation of particles in standing wave fields
CN107614097B (zh) 2015-05-27 2020-02-14 联邦科学与工业研究组织 金属-有机框架的生产
WO2016187669A1 (en) 2015-05-27 2016-12-01 Commonwealth Scientific And Industrial Research Organisation Separation of metal-organic frameworks
EP3303558B1 (en) 2015-05-29 2022-05-25 Boehringer Ingelheim International GmbH Cell-controlled perfusion in continuous culture
US20180148763A1 (en) 2015-06-01 2018-05-31 Shimadzu Corporation Method for quantifying monoclonal antibody
EP3307774A1 (en) 2015-06-09 2018-04-18 Institut Pasteur Monoclonal antibodies specific for serogroup x of n. meningitidis and uses thereof in diagnosis
US10161926B2 (en) 2015-06-11 2018-12-25 Flodesign Sonics, Inc. Acoustic methods for separation of cells and pathogens
RU2752834C2 (ru) 2015-06-18 2021-08-09 Те Брод Инститьют, Инк. Мутации фермента crispr, уменьшающие нецелевые эффекты
EP3666895A1 (en) 2015-06-18 2020-06-17 The Broad Institute, Inc. Novel crispr enzymes and systems
AU2016279062A1 (en) 2015-06-18 2019-03-28 Omar O. Abudayyeh Novel CRISPR enzymes and systems
EP3311173A2 (en) 2015-06-18 2018-04-25 Thomas Mole Herd Methods of characterising cancer
US9957501B2 (en) 2015-06-18 2018-05-01 Sangamo Therapeutics, Inc. Nuclease-mediated regulation of gene expression
US10648020B2 (en) 2015-06-18 2020-05-12 The Broad Institute, Inc. CRISPR enzymes and systems
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
US9663756B1 (en) 2016-02-25 2017-05-30 Flodesign Sonics, Inc. Acoustic separation of cellular supporting materials from cultured cells
EP3310848A1 (en) 2015-06-22 2018-04-25 Proxonix AS Method for making a body with arranged particles using acoustic waves
US9613615B2 (en) 2015-06-22 2017-04-04 Sony Corporation Noise cancellation system, headset and electronic device
US9368110B1 (en) 2015-07-07 2016-06-14 Mitsubishi Electric Research Laboratories, Inc. Method for distinguishing components of an acoustic signal
JP5923205B1 (ja) 2015-07-07 2016-05-24 日立アロカメディカル株式会社 超音波探触子
EP3319739B1 (en) 2015-07-09 2021-03-31 Flodesign Sonics Inc. Non-planar and non-symmetrical piezolectric crystals and reflectors
EP3322297A4 (en) 2015-07-13 2019-04-17 Sangamo Therapeutics, Inc. RELEASE METHOD AND COMPOSITIONS FOR NUCLEASE-DERIVED GENOMINE ENGINEERING
JP2018527903A (ja) 2015-07-22 2018-09-27 スカラー ロック インコーポレイテッドＳｃｈｏｌａｒ Ｒｏｃｋ，Ｉｎｃ． Ｇｄｆ１１結合タンパク質およびその使用
CA3005845A1 (en) 2015-07-28 2017-02-02 Flodesign Sonics, Inc. Acoustic affinity separation
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
CN108472314A (zh) 2015-07-31 2018-08-31 明尼苏达大学董事会 修饰的细胞和治疗方法
CN105087788B (zh) 2015-08-03 2018-11-23 上海白泽医疗器械有限公司 一种分选人细胞的免疫磁珠、其制备及切除方法
EP3332160B1 (en) 2015-08-03 2023-09-27 Arlenko Pty Ltd Fire collar
EP3331993A1 (en) 2015-08-05 2018-06-13 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Use of dermatopontin for maintaining hematopoietic stem and/or progenitor cells in culture
CN105384825B (zh) 2015-08-11 2018-06-01 南京传奇生物科技有限公司 一种基于单域抗体的双特异性嵌合抗原受体及其应用
WO2017027519A1 (en) 2015-08-11 2017-02-16 Apocell, Inc. Systems and methods for isolating target particles and their use in diagnostic, prognostic, and therapeutic, methods
US10261078B2 (en) 2015-08-17 2019-04-16 National Technology & Engineering Solutions Of Sandia, Llc Shear horizontal surface acoustic wave (SH-SAW) resonators and arrays thereof
JP7091238B2 (ja) 2015-08-20 2022-06-27 ジェネンテック， インコーポレイテッド Ｆｋｐａの精製、及び組換えポリペプチドの産生のためのその使用
WO2017032777A1 (en) 2015-08-24 2017-03-02 Cellectis Chimeric antigen receptors with integrated controllable functions
WO2017035249A1 (en) 2015-08-24 2017-03-02 Trustees Of Boston University ANTI-DEspR MONOCLONAL ANTIBODY TARGETED THERAPY AND IMAGING FOR CANCER AND STROKE
LT3341116T (lt) 2015-08-27 2022-05-25 President And Fellows Of Harvard College Rūšiavimo naudojant akustines bangas būdas
GB201515339D0 (en) 2015-08-28 2015-10-14 Ge Healthcare Bio Sciences Ab A seperation matrix and a method of seperating antibodies
JP2018526013A (ja) 2015-09-01 2018-09-13 イネイト・テューマー・イミュニティ・インコーポレイテッドＩｎｎａｔｅ Ｔｕｍｏｒ Ｉｍｍｕｎｉｔｙ， Ｉｎｃ． 免疫または免疫抑制性サイトカインに対する抵抗性が増大した免疫細胞およびその使用
WO2017041102A1 (en) 2015-09-04 2017-03-09 Flodesign Sonics, Inc. Acoustic blood separation processes and devices
WO2017044864A1 (en) 2015-09-09 2017-03-16 Revivicor, Inc Multi-transgenic pig for xenotransplantation
EP3347098B1 (en) 2015-09-09 2022-01-05 Virginia Commonwealth University Targeting mda-5 activation for cancer immunotherapy
US10155222B2 (en) 2015-09-17 2018-12-18 Carnegie Mellon University Device for the separation of particles using a bulk acoustic wave field
US20190054112A1 (en) 2015-09-18 2019-02-21 Moderna Therapeutics, Inc. Polynucleotide formulations for use in the treatment of renal diseases
CN106554010A (zh) 2015-09-25 2017-04-05 苏州烯时代石墨烯科技有限公司 工业化制备大尺寸石墨烯的方法
USD787630S1 (en) 2015-10-06 2017-05-23 Flodesign Sonics, Inc. U-turn acoustic separation device
KR102549704B1 (ko) 2015-10-07 2023-06-30 후지레비오 가부시키가이샤 Pivka-ii의 측정 방법, 및 pivka-ii 면역측정 시약 또는 키트의 제조 방법
US10224015B2 (en) 2015-10-09 2019-03-05 Jeffrey James Hsu Stringless bowed musical instrument
EP3362102A1 (en) 2015-10-14 2018-08-22 Life Technologies Corporation Ribonucleoprotein transfection agents
EP3386652B1 (en) 2015-10-14 2022-06-22 Sonosep Technologies Inc. Methods, systems, and apparatuses for acoustically separating and washing particles
WO2017070110A1 (en) 2015-10-19 2017-04-27 Cold Genesys, Inc. Methods of treating solid or lymphatic tumors by combination therapy
CA3001351A1 (en) 2015-10-21 2017-04-27 Editas Medicine, Inc. Crispr/cas-related methods and compositions for treating hepatitis b virus
EP3365441A1 (en) 2015-10-22 2018-08-29 The Broad Institute Inc. Type vi-b crispr enzymes and systems
BR112018008250B1 (pt) 2015-10-23 2022-10-04 3M Innovative Properties Company Sequência de meio filtrante, dispositivo filtrante e método de fabricação de um fluido biológico
TW201729810A (zh) 2015-10-26 2017-09-01 札爾科製藥公司 嘧啶組成物、其超純組成物及鹽類、製造彼之方法、及使用彼於治療組織胺h4受體(h 4 )仲介之疾病與病情之方法
JP7408284B2 (ja) 2015-10-30 2024-01-05 エディタス・メディシン、インコーポレイテッド 単純ヘルペスウイルスを治療するためのｃｒｉｓｐｒ／ｃａｓ関連方法及び組成物
WO2017079674A1 (en) 2015-11-04 2017-05-11 Northeastern University Systems for producing cellular immunotherapeutics and methods of use thereof
SG10201509280YA (en) 2015-11-11 2017-06-29 Singapore Univ Of Tech And Design Microfluidic particle manipulation
WO2017083681A1 (en) 2015-11-13 2017-05-18 The University Or North Carolina At Chapel Hill Optimized crosslinkers for trapping a target on a substrate
EP3359676B1 (en) 2015-12-14 2021-02-24 GenomeFrontier Therapeutics, Inc. Transposon system, kit comprising the same, and uses thereof
JP2019506143A (ja) 2015-12-14 2019-03-07 ベリカム ファーマシューティカルズ， インコーポレイテッド 治療用細胞の活性化及び排除のための二重コントロール
US11053788B2 (en) 2015-12-16 2021-07-06 Saudi Arabian Oil Company Acoustic downhole oil-water separation
US10648460B2 (en) 2015-12-16 2020-05-12 The University Of Hong Kong Nanomotor propulsion
JP7065036B2 (ja) 2015-12-17 2022-05-11 モダーナティエックス・インコーポレイテッド メチルマロニルＣｏＡムターゼをコードするポリヌクレオチド
EA201891212A1 (ru) 2015-12-18 2019-01-31 Сангамо Терапьютикс, Инк. Адресная дезорганизация клеточного рецептора гкгс
SI3394266T1 (sl) 2015-12-22 2021-08-31 Xl-Protein Gmbh Nukleinske kisline, ki kodirajo ponavljajoča se aminokislinska zaporedja, bogata s prolinskimi in alaninskimi ostanki, ki imajo manj ponavljajočih se nukleotidnih zaporedij
US10648900B2 (en) 2015-12-23 2020-05-12 Becton, Dickinson And Company Multi-color flow cytometric analysis of samples with low cell numbers
BR112018013065A2 (pt) 2015-12-28 2018-12-11 Intellia Therapeutics Inc composições e métodos para o tratamento de hemoglobinopatias
CA3010612A1 (en) 2016-01-08 2017-07-13 Oncobiologics, Inc. Methods for separating isoforms of monoclonal antibodies
KR20180096793A (ko) 2016-01-14 2018-08-29 안톤 바우어 생산 세포주를 제조하는 방법
US10842523B2 (en) 2016-01-15 2020-11-24 Ethicon Llc Modular battery powered handheld surgical instrument and methods therefor
KR20180101540A (ko) 2016-01-15 2018-09-12 이투빅스 코포레이션 T 세포 면역요법을 위한 방법 및 조성물
WO2017127686A1 (en) 2016-01-22 2017-07-27 Carnegie Mellon University Three-dimensional acoustic manipulation of cells
US10973852B2 (en) 2016-01-25 2021-04-13 Nantcell, Inc. NK cells with altered CXCL12/CXCR4 signaling
US20190031780A1 (en) 2016-01-27 2019-01-31 Siamab Therapeutics, Inc. Compositions and methods for targeting cancer stem cells
WO2017133175A1 (en) 2016-02-04 2017-08-10 Nanjing Legend Biotech Co., Ltd. Engineered mammalian cells for cancer therapy
WO2017148928A1 (en) 2016-03-01 2017-09-08 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Means and methods for treating herpesvirus infection
WO2017152015A1 (en) 2016-03-04 2017-09-08 Editas Medicine, Inc. Crispr-cpf1-related methods, compositions and components for cancer immunotherapy
CA3016841A1 (en) 2016-03-06 2017-09-14 WindplusSonne GmbH Method and device for separating and/or cleaning aerosols and solid material particles and fibers from gases as well as solid material particles and fibers from liquid materials by acoustophoresis
US11497781B2 (en) 2016-03-10 2022-11-15 Cg Oncology, Inc. Methods of treating bladder cancer by combination therapy comprising the oncolytic adenovirus CG0070 and an immune checkpoint inhibitor
BR112018068354A2 (pt) 2016-03-11 2019-01-15 Bluebird Bio Inc células efetoras imunológicas do genoma editado
WO2017161384A1 (en) 2016-03-12 2017-09-21 Flodesign Sonics, Inc. Multi-stage acoustophoresis device
EP3219800A1 (en) 2016-03-15 2017-09-20 Max-Delbrück-Centrum Für Molekulare Medizin A transposon-based transfection system for primary cells
CA3015162A1 (en) 2016-03-15 2017-09-21 Cancer Research Technology Limited Antibodies and related molecules and uses thereof
WO2017157426A1 (en) 2016-03-15 2017-09-21 Centre National De La Recherche Scientifique Acoustic tweezers
WO2017160964A1 (en) 2016-03-15 2017-09-21 Washington University High throughput acoustic particle separation methods and devices
WO2017160991A1 (en) 2016-03-16 2017-09-21 Lavieu Gregory G Methods, systems and devices for selection and generation of genome edited clones
CN108779469A (zh) 2016-03-25 2018-11-09 基因体先驱生医股份有限公司 用以建构转位子的套组及其用途
EP3433363A1 (en) 2016-03-25 2019-01-30 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
WO2017172644A2 (en) 2016-03-28 2017-10-05 The Charles Stark Draper Laboratory, Inc. Bacteria identification and antibiotic susceptibility profiling device
WO2017173005A1 (en) 2016-03-30 2017-10-05 Sapphire Energy, Inc. Modified rubisco large subunit proteins
TN2018000337A1 (en) 2016-04-01 2020-01-16 Amgen Inc Chimeric receptors to flt3 and methods of use thereof
WO2017173384A1 (en) 2016-04-01 2017-10-05 Kite Pharma, Inc. Chimeric receptors and methods of use thereof
US20190119387A1 (en) 2016-04-05 2019-04-25 Glaxosmithkline Intellectual Property Development Limited Inhibition of tgfbeta in immunotherapy
SG11201808705XA (en) 2016-04-06 2018-11-29 Univ Singapore Technology & Design Particle manipulation
CA3020330A1 (en) 2016-04-07 2017-10-12 Bluebird Bio, Inc. Chimeric antigen receptor t cell compositions
WO2017180665A2 (en) 2016-04-12 2017-10-19 Edward Perkins Methods for creatings synthetic chromosomes expressing biosynthetic pathways and uses thereof
RU2018139465A (ru) 2016-04-12 2020-05-13 СИНПЛОЙД БАЙОТЕК, ЭлЭлСи Способы создания синтетических хромосом, имеющих генные регуляторные системы, и их применение
CA3020993A1 (en) 2016-04-14 2017-10-19 Bluebird Bio, Inc. Salvage chimeric antigen receptor systems
US10932829B2 (en) 2016-04-14 2021-03-02 Orthoxel Dac Intramedullary nail system
US11286478B2 (en) 2016-04-19 2022-03-29 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
WO2017189308A1 (en) 2016-04-19 2017-11-02 The Broad Institute Inc. Novel crispr enzymes and systems
KR102670601B1 (ko) 2016-04-19 2024-05-29 더 브로드 인스티튜트, 인코퍼레이티드 신규한 crispr 효소 및 시스템
KR102529012B1 (ko) 2016-04-22 2023-05-09 크라제 메디컬 씨오 리미티드 세포성 면역요법을 위한 조성물 및 방법
WO2017186718A1 (en) 2016-04-25 2017-11-02 Universität Basel Allele editing and applications thereof
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
EP3452499A2 (en) 2016-05-06 2019-03-13 Juno Therapeutics, Inc. Genetically engineered cells and methods of making the same
MA45053A (fr) 2016-05-18 2019-03-27 Modernatx Inc Polynucléotides codant pour un régulateur de conductance transmembranaire de fibrose kystique pour le traitement de la fibrose kystique
MA45041A (fr) 2016-05-18 2019-03-27 Modernatx Inc Polynucléotides codant pour la galactose-1-phosphate uridylyltransférase destinés au traitement de la galactosémie de type 1
MY201498A (en) 2016-05-18 2024-02-27 Modernatx Inc Polynucleotides encoding citrin for the treatment of citrullinemia type 2
CA3024624A1 (en) 2016-05-18 2017-11-23 Modernatx, Inc. Polynucleotides encoding porphobilinogen deaminase for the treatment of acute intermittent porphyria
EP3458107B1 (en) 2016-05-18 2024-03-13 ModernaTX, Inc. Polynucleotides encoding jagged1 for the treatment of alagille syndrome
SI3458083T1 (sl) 2016-05-18 2023-03-31 Modernatx, Inc. Polinukleotidi, ki kodirajo interlevkin-12 (IL12) in njihova uporaba
CA3024507A1 (en) 2016-05-18 2017-11-23 Modernatx, Inc. Polynucleotides encoding .alpha.-galactosidase a for the treatment of fabry disease
US20200316586A1 (en) 2016-05-24 2020-10-08 Centre National De La Recherche Scientifique Acoustic tweezers
EP3463433A1 (en) 2016-05-25 2019-04-10 Institut National de la Sante et de la Recherche Medicale (INSERM) Methods and compositions for treating cancers
WO2017207589A1 (en) 2016-06-01 2017-12-07 Kws Saat Se Hybrid nucleic acid sequences for genome engineering
US10829402B2 (en) 2016-06-07 2020-11-10 Evoqua Water Technologies Llc Ballasted solids treatment system and method
EP3468351B1 (en) 2016-06-13 2020-09-09 Lic Automation Limited A livestock management system and method of operation
WO2017218519A1 (en) 2016-06-13 2017-12-21 Bluebird Bio, Inc. Gene therapy of neuronal ceroid lipofuscinoses
CN114176043B (zh) 2016-06-14 2024-04-23 明尼苏达大学董事会 用于治疗疾病的遗传修饰的细胞、组织和器官
EP3257600A1 (en) 2016-06-15 2017-12-20 Maquinaria GEKA, S.A. Notching tool, use and method
WO2017222777A1 (en) 2016-06-21 2017-12-28 Becton, Dickinson And Company Devices and methods for acoustic particle separation
US10800899B2 (en) 2016-06-22 2020-10-13 Yunuen Montelongo Reversible optical assembly of composites
WO2017220767A1 (en) 2016-06-24 2017-12-28 Sciencons AS Preparation of 212pb labeled monoclonal antibodies
WO2018002036A1 (en) 2016-06-28 2018-01-04 Zaklady Farmaceutyczne Polpharma Sa Recombinant production of monoclonal antibodies
US20180000870A1 (en) 2016-06-29 2018-01-04 Applied Biologics, Llc Methods of forming amniotic fluid-derived preparations
US20180015128A1 (en) 2016-06-29 2018-01-18 Applied Biologics, Llc Amniotic Fluid-Derived Preparation with a Standardized Biologic Activity
US20210222164A1 (en) 2016-06-29 2021-07-22 The Broad Institute, Inc. Crispr-cas systems having destabilization domain
KR102496333B1 (ko) 2016-06-30 2023-02-07 삼성전자주식회사 식기 세척기 및 그 제어 방법
CA3030156A1 (en) 2016-07-07 2018-01-11 Iovance Biotherapeutics, Inc. Programmed death 1 ligand 1 (pd-l1) binding proteins and methods of use thereof
US10245064B2 (en) 2016-07-12 2019-04-02 Ethicon Llc Ultrasonic surgical instrument with piezoelectric central lumen transducer
WO2018013558A1 (en) 2016-07-12 2018-01-18 Life Technologies Corporation Compositions and methods for detecting nucleic acid regions
US11134864B2 (en) 2016-07-13 2021-10-05 Alexei L. Vyssotski Tracking method and system for small animal research
AU2017295720B2 (en) 2016-07-13 2021-07-22 Vertex Pharmaceuticals Incorporated Methods, compositions and kits for increasing genome editing efficiency
EP3484613B1 (en) 2016-07-14 2021-02-24 Puridify Ltd. Functionalised chromatography medium comprising polymer nanofibres and process of preparation thereof
WO2018014174A1 (en) 2016-07-19 2018-01-25 General Electric Company Ultrasonic separation of a production stream
EP3487982A1 (en) 2016-07-21 2019-05-29 Celyad Method and apparatus for automated independent parallel batch-processing of cells
JP2019522008A (ja) 2016-07-25 2019-08-08 セルラー アプローチズ，インク． 治療方法で使用される自己由来および同種異系のマクロファージと単球
JP2019525759A (ja) 2016-07-25 2019-09-12 ブルーバード バイオ， インコーポレイテッド Ｂｃｌ１１ａホーミングエンドヌクレアーゼバリアント、組成物、および使用方法
WO2018021920A1 (en) 2016-07-27 2018-02-01 The University Of Canterbury Maskless speech airflow measurement system
CN109890619B (zh) 2016-07-27 2021-01-26 哈佛学院院长及董事 用于声泳打印的设备和方法
WO2018022930A1 (en) 2016-07-27 2018-02-01 The Board Of Trustees Of The Leland Stanford Junior University Immolative cell-penetrating complexes for nucleic acid delivery
ES2908736T3 (es) 2016-07-28 2022-05-03 Charles Stark Draper Laboratory Inc Separación acústica para bioprocesamiento
GB201613078D0 (en) 2016-07-28 2016-09-14 Univ Oxford Innovation Ltd Stem cells and cancer
WO2018023114A1 (en) 2016-07-29 2018-02-01 Ohio State Innovation Foundation Expression of pten-long with ocolytic viruses
EP3490694A4 (en) 2016-07-31 2020-04-15 Ancera Corp. MULTI-LAYER DISPOSABLE CARTRIDGE FOR FERROFLUID BASED ASSAYS AND METHOD OF USE
CN109715789B (zh) 2016-08-01 2023-04-04 刘扶东 内部封定脂囊泡
CA3032688A1 (en) 2016-08-02 2018-02-08 Nantcell, Inc. Transfection of dendritic cells and methods therefor
EP3493907B1 (en) 2016-08-02 2023-10-04 IMEC vzw Method and arrangement for focusing objects in a flow
KR102561368B1 (ko) 2016-08-03 2023-08-01 론자 워커스빌 아이엔씨. 실질적으로 코아굴로겐이 없는 리물루스 아메보사이트 라이세이트를 이용한 내독소 검출방법
RU2022103870A (ru) 2016-08-12 2022-03-04 Тулджен Инкорпорейтед Модифицированный иммунорегуляторный элемент и изменение иммунитета посредством этого элемента
US10160061B2 (en) 2016-08-15 2018-12-25 The Aerospace Corporation Systems and methods for modifying acoustic waves based on selective heating
MA45996A (fr) 2016-08-16 2021-06-02 Bluebird Bio Inc Variants d'endonucléase de homing du récepteur alpha de l'il-10, compositions et méthodes d'utilisation associées
CA3034208A1 (en) 2016-08-16 2018-02-22 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
MY196321A (en) 2016-08-16 2023-03-24 Regeneron Pharma Methods for Quantitating Individual Antibodies From a Mixture
CN109642868B (zh) 2016-08-19 2022-03-15 国立大学法人香川大学 光学特性测定装置和光学特性测定方法
WO2018035423A1 (en) 2016-08-19 2018-02-22 Bluebird Bio, Inc. Genome editing enhancers
EP3500244A4 (en) 2016-08-22 2020-05-13 Codiak BioSciences, Inc. METHOD FOR SUPPRESSING THE DELIVERY OF EXOSOMES TO LIVER AND SPLEEN
CN109863238A (zh) 2016-08-23 2019-06-07 弗洛设计声能学公司 声学生物反应器方法
US10952759B2 (en) 2016-08-25 2021-03-23 Ethicon Llc Tissue loading of a surgical instrument
US10736649B2 (en) 2016-08-25 2020-08-11 Ethicon Llc Electrical and thermal connections for ultrasonic transducer
WO2018045284A1 (en) 2016-09-02 2018-03-08 The General Hospital Corporation Methods and systems for non-contact construction of an internal structure
EP4248979A3 (en) 2016-09-08 2023-12-20 2seventy bio, Inc. Pd-1 homing endonuclease variants, compositions, and methods of use
EP3512540A1 (en) 2016-09-14 2019-07-24 Université Catholique de Louvain Modified vsv-g and vaccines thereof
WO2018057825A1 (en) 2016-09-23 2018-03-29 The Curators Of The University Of Missouri Antigen-specific immune modulation
WO2018058275A1 (zh) 2016-09-27 2018-04-05 深圳智乐信息科技有限公司 一种使用移动终端的智能驾驶方法及系统
WO2018063284A1 (en) 2016-09-30 2018-04-05 Intel Corporation Film bulk acoustic resonator (fbar) devices with 2deg bottom electrode
US10979012B2 (en) 2016-09-30 2021-04-13 Intel Corporation Single-flipped resonator devices with 2DEG bottom electrode
WO2018067614A1 (en) 2016-10-04 2018-04-12 Massachusetts Institute Of Technology Fiber microfluidics
WO2018081476A2 (en) 2016-10-27 2018-05-03 Intima Bioscience, Inc. Viral methods of t cell therapy
US10830738B2 (en) 2016-11-14 2020-11-10 University Of Alberta Ultrasensitive high Q-factor AT-cut-quartz crystal microbalance femtogram mass sensor
US20180136167A1 (en) 2016-11-16 2018-05-17 Battelle Energy Alliance, Llc Acoustic measurement infrastructure method and system for process monitoring, diagnostics, and prognostics
CN117720665A (zh) 2016-11-17 2024-03-19 2赛文缇生物公司 TGFβ信号转换器
FR3058644B1 (fr) 2016-11-17 2022-04-22 Commissariat Energie Atomique Procede et dispositif de concentration de particules par acoustophorese
GB201619559D0 (en) 2016-11-18 2017-01-04 Univ Oxford Innovation Ltd Acoustic excitation and detection of spin waves
WO2018098671A1 (en) 2016-11-30 2018-06-07 China Agricultural University A method for crispr library screening
WO2018102752A1 (en) 2016-12-02 2018-06-07 Cartesian Therapeutics, Inc. Cancer immuno therapy with highly enriched cd8+ chimeric antigen receptor t cells
JP6414994B2 (ja) 2016-12-06 2018-10-31 学校法人同志社 液晶可変焦点レンズおよび焦点距離制御方法
WO2018106163A1 (en) 2016-12-07 2018-06-14 Dirac Research Ab Audio precompensation filter optimized with respect to bright and dark zones
JP7483374B2 (ja) 2016-12-15 2024-05-15 ベックマン コールター， インコーポレイテッド 細胞洗浄装置及び方法
WO2018112335A1 (en) 2016-12-16 2018-06-21 Dolby Laboratories Licensing Corporation Audio speaker with full-range upward firing driver for reflected sound projection
US9909313B1 (en) 2017-01-19 2018-03-06 Austin M. Grubbs Composite materials, methods of making composite materials, and enclosures constructed from composite materials
MX2019008844A (es) 2017-01-26 2019-09-10 Sangamo Therapeutics Inc Ingenieria de celulas b.
WO2018140845A2 (en) 2017-01-27 2018-08-02 Duke University Bi-specific antibodies to cd64 and a disease antigen
JP2020505074A (ja) 2017-01-30 2020-02-20 カー・ヴェー・エス ザート エス・エー ウント コー． カー・ゲー・アー・アーＫＷＳ ＳＡＡＴ ＳＥ ＆ Ｃｏ． ＫＧａＡ ゲノム工学のためのエンドヌクレアーゼに対する修復鋳型の結合
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
US20180223256A1 (en) 2017-02-09 2018-08-09 University Of Rochester Modified t cells and their uses in treating cancer
CA2994643A1 (en) 2017-02-14 2018-08-14 Bart Lipkens Bioreactor using acoustic standing waves
MX2019009753A (es) 2017-02-16 2019-12-18 Firststring Res Inc Composicion y metodos para prevenir lesion por radiacion y promover la regeneracion de tejidos.
AU2018222735B2 (en) 2017-02-17 2023-04-27 George Todaro Use of TGF alpha for the treatment of diseases and disorders
GB201703116D0 (en) 2017-02-27 2017-04-12 Ge Healthcare Bioprocess R&D Ab A seperation matrix and a method of seperating antibodies
WO2018160548A1 (en) 2017-02-28 2018-09-07 Cardiodx, Inc. Markers for coronary artery disease and uses thereof
WO2018161026A1 (en) 2017-03-03 2018-09-07 Obsidian Therapeutics, Inc. Il15 compositions and methods for immunotherapy
SG11201907922PA (en) 2017-03-03 2019-09-27 Obsidian Therapeutics Inc Cd19 compositions and methods for immunotherapy
AU2018226824A1 (en) 2017-03-03 2019-09-19 Seagen Inc. Glycan-interacting compounds and methods of use
US11229809B2 (en) 2017-03-03 2022-01-25 California Institute Of Technology Selective disruption of neoplastic cells via resonant harmonic excitation
JP2020511528A (ja) 2017-03-03 2020-04-16 オブシディアン セラピューティクス， インコーポレイテッド 免疫療法のための組成物及び方法
WO2018161038A1 (en) 2017-03-03 2018-09-07 Obsidian Therapeutics, Inc. Il12 compositions and methods for immunotherapy
EP3372813B8 (en) 2017-03-07 2021-04-07 Raytheon Technologies Corporation Multi degree of freedom flutter damper
US10415506B2 (en) 2017-03-07 2019-09-17 United Technologies Corporation Multi degree of freedom flutter damper
CN106932370B (zh) 2017-03-07 2019-08-13 中国农业科学院油料作物研究所 同步检测五种真菌毒素的层析时间分辨荧光试剂盒及应用
US10428765B2 (en) 2017-03-07 2019-10-01 United Technologies Corporation Asymmetric multi degree of freedom flutter damper
EP3372814B1 (en) 2017-03-07 2022-05-04 Raytheon Technologies Corporation Asymmetric multi degree of freedom flutter damper
US11643342B2 (en) 2017-03-09 2023-05-09 B.G. Negev Technologies & Applications Ltd., At Ben-Gurion University Process and apparatus for purifying liquid
MX2019011775A (es) 2017-03-31 2020-02-05 Quest Diagnostics Invest Llc Metodos para la cuantificacion de insulina y peptido c.
WO2018202691A1 (en) 2017-05-02 2018-11-08 Linnaeus University Method for performing a bioleaching process of chalcopyrite
US11358140B2 (en) 2017-05-16 2022-06-14 Autonomous Medical Devices Inc. Apparatus for automatic sampling of biological species employing an amplification with a magnetic nanoparticle and propulsion method
WO2018215686A1 (es) 2017-05-24 2018-11-29 Universidad De Granada Aislamiento de células de origen epitelial circulantes en sangre periférica
UY37758A (es) 2017-06-12 2019-01-31 Novartis Ag Método de fabricación de anticuerpos biespecíficos, anticuerpos biespecíficos y uso terapéutico de dichos anticuerpos
GB2588249B (en) 2017-06-12 2023-01-04 Sinai Health Sys Allograft tolerance without the need for systemic immune suppression
MX2019014960A (es) 2017-06-12 2020-08-06 Obsidian Therapeutics Inc Composiciones de fosfodiesterasa de tipo 5 (pde5) y métodos de inmunoterapia.
WO2018232045A1 (en) 2017-06-13 2018-12-20 Flodesign Sonics, Inc. Driver and control for variable impedance load
US20200131498A1 (en) 2017-06-14 2020-04-30 Modernatx, Inc. Polynucleotides encoding methylmalonyl-coa mutase
US10987462B2 (en) 2017-06-14 2021-04-27 The Charles Stark Draper Laboratory, Inc. Acoustophoresis device having improved dimensions
WO2018236708A1 (en) 2017-06-19 2018-12-27 Massachusetts Institute Of Technology SYSTEMS AND METHODS FOR MEASURING PARTICLE PROPERTIES
GB201709838D0 (en) 2017-06-20 2017-08-02 Univ Oslo Hf Method of modulating immune cells
WO2018235228A1 (ja) 2017-06-22 2018-12-27 株式会社島津製作所 抗原または抗抗体が結合したモノクローナル抗体の定量方法
US10781435B2 (en) 2017-06-22 2020-09-22 Catalyst Biosciences, Inc. Modified membrane type serine protease 1 (MTSP-1) polypeptides and methods of use
WO2018237239A1 (en) 2017-06-23 2018-12-27 Texas Tech University System METHOD AND DEVICE FOR HIGH-PERFORMANCE, LABEL-FREE HOLOGRAPHIC SCREENING AND TUMOR CELL ENUMERATION IN BLOOD
US20210355448A1 (en) 2017-06-26 2021-11-18 City Of Hope Methods of islet cell culture
WO2019005866A1 (en) 2017-06-26 2019-01-03 The Broad Institute, Inc. NEW TYPE VI CRISPR ORTHOLOGISTS AND ASSOCIATED SYSTEMS
TWI704923B (zh) 2017-06-26 2020-09-21 台灣基督長老教會馬偕醫療財團法人馬偕紀念醫院 包含胞外囊泡之製劑、用以製備該製劑之方法及其用途
US10535425B2 (en) 2017-06-28 2020-01-14 Perceptimed, Inc. Inventory management
WO2019006418A2 (en) 2017-06-30 2019-01-03 Intima Bioscience, Inc. ADENO-ASSOCIATED VIRAL VECTORS FOR GENE THERAPY
US20190004806A1 (en) 2017-06-30 2019-01-03 Qualcomm Incorporated Branch prediction for fixed direction branch instructions
WO2019005155A1 (en) 2017-06-30 2019-01-03 Hewlett-Packard Development Company, L.P. IMAGING DEVICE COMPRISING AN AUDIO PART
EP3421975A1 (en) 2017-06-30 2019-01-02 Centre National De La Recherche Scientifique Methods and device for manipulating objects
US20220233588A1 (en) 2017-06-30 2022-07-28 Cellectis Cellular immunotherapy for repetitive administration
GB201710620D0 (en) 2017-07-03 2017-08-16 Glaxosmithkline Intellectual Property Ltd Targeted protein degradation
WO2019010422A1 (en) 2017-07-07 2019-01-10 The Broad Institute, Inc. ANTIVIRAL THERAPY BASED ON THE CRISPR SYSTEM
GB201710973D0 (en) 2017-07-07 2017-08-23 Avacta Life Sciences Ltd Scaffold proteins
EP3425386B1 (en) 2017-07-07 2022-04-13 Université de Strasbourg Online apparatus for in-depth characterization of antibody drug conjugates
WO2019018423A1 (en) 2017-07-17 2019-01-24 The Broad Institute, Inc. NEW TYPE VI CRISPR ORTHOLOGISTS AND ASSOCIATED SYSTEMS
BR112020001132A2 (pt) 2017-07-18 2020-07-21 Calimmune, Inc. interruptor modulável para seleção de células doadoras modificadas
US20200164060A1 (en) 2017-07-21 2020-05-28 Fred Hutchinson Cancer Research Center Herpes simplex virus vaccine epitopes specifically recognized by tissue resident memory t cells
US20210123038A1 (en) 2017-07-26 2021-04-29 Flodesign Sonics, Inc. Acoustic Transducer Automated Start and Run
EP3658125A4 (en) 2017-07-28 2021-04-28 National University of Singapore BIOMOLECULAR COMPOSITES INCLUDING MODIFIED CELL PHANTOMS
WO2019025984A1 (en) 2017-07-31 2019-02-07 Reflection Biotechnologies Limited CELLULAR MODELS AND THERAPIES FOR OCULAR DISEASES
CN111182951A (zh) 2017-08-01 2020-05-19 指南针制药有限责任公司 过滤和层析荚以及使用其的方法
US11266984B2 (en) 2017-08-03 2022-03-08 The Board Of Trustees Of The Leland Stanford Junior University Massive microfluidics for multiplexed counting
EP3437740A1 (en) 2017-08-04 2019-02-06 Nokia Technologies Oy Apparatus and method for positioning particles inside a channel
EP3665271A4 (en) 2017-08-08 2021-04-28 Sangamo Therapeutics, Inc. CELL TARGETING MEDIA BY A CHEMICAL ANTIGEN RECEPTOR
WO2019036382A1 (en) 2017-08-15 2019-02-21 Progenity Inc. INFLAMMATORY DISEASE TREATMENT INVOLVING AN INGREDIENT DEVICE FOR RELEASING AN IMMUNOMODULATOR
US10969324B2 (en) 2017-08-16 2021-04-06 Washington University Synthesis, post-modification and separation of biologics using acoustically confined substrates
EP3675835A4 (en) 2017-08-28 2021-06-09 Matthias Wagner MICROFLUIDIC LASER ACTIVATED INTRACELLULAR DELIVERY SYSTEMS AND METHODS
US11085902B2 (en) 2017-08-29 2021-08-10 Massachusetts Institute Of Technology Optical breakdown acoustic transducer
WO2019041344A1 (en) 2017-09-04 2019-03-07 Hebei University Of Science And Technology METHODS AND COMPOSITIONS FOR THE TRANSFECTION OF SINGLE STRANDED DNA
CA3074872A1 (en) 2017-09-07 2019-03-14 The Board Of Trustees Of The Leland Stanford Junior University Nuclease systems for genetic engineering
EP3678685A1 (en) 2017-09-07 2020-07-15 Vib Vzw Mixed-lineage kinase domain-like protein in immunotherapeutic cancer control
EP3679143A1 (en) 2017-09-08 2020-07-15 Life Technologies Corporation Methods for improved homologous recombination and compositions thereof
US12016314B2 (en) 2017-09-08 2024-06-25 Ohio State Innovation Foundation MicroRNA inhibitor therapy in systemic lupus erythematosus
WO2019051106A1 (en) 2017-09-08 2019-03-14 The Charles Stark Draper Laboratory Inc. DETECTION AND IDENTIFICATION OF BACTERIA AND DETERMINATION OF ANTIBIOTIC SENSITIVITY USING BACTERIOPHAGES AND MOLECULES REPORTERS
JP7274228B2 (ja) 2017-09-08 2023-05-16 ネオペプ ファーマ ゲーエムベーハー ウント コンパニー カーゲー 病気の治療のためのポリペプチド
WO2019051316A1 (en) 2017-09-08 2019-03-14 Life Technologies Corporation ENHANCED APPROVAL RECOMBINATION METHODS AND COMPOSITIONS THEREOF
EP3678710A4 (en) 2017-09-08 2021-06-09 Generation Bio Co. MODIFIED CLOSED-END DNA (CEDNA)
US20190076473A1 (en) 2017-09-14 2019-03-14 Cellular Approaches, Inc. Autologous and allogenic macrophages and monocytes for use in therapeutic methods
WO2019055817A1 (en) 2017-09-14 2019-03-21 The Regents Of The University Of California EX VIVO CONSERVATION AND EXPANSION OF STEM CELLS
CN114533863A (zh) 2017-09-15 2022-05-27 凯德药业股份有限公司 用于进行具有监管链和身份链生物样品跟踪的患者特异性免疫疗法规程的方法和系统
CN111093715A (zh) 2017-09-18 2020-05-01 儿童医院医疗中心 强绝缘子和其在基因递送中的用途
DK3456821T4 (da) 2017-09-19 2024-04-15 Deutsches Krebsforsch Ikke-integrerende dna-vektorer til genetisk modifikation af celler
WO2019057774A1 (en) 2017-09-19 2019-03-28 Deutsches Krebsforschungszentrum NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS
US20200230221A1 (en) 2017-09-19 2020-07-23 Massachusetts Institute Of Technology Compositions for chimeric antigen receptor t cell therapy and uses thereof
IT201700105911A1 (it) 2017-09-21 2019-03-21 Menarini Silicon Biosystems Spa Metodo ed apparato per la riduzione del volume di un campione
IT201700105948A1 (it) 2017-09-21 2019-03-21 Menarini Silicon Biosystems Spa Metodo e sistema microfluidico per il recupero di particelle
WO2019060779A1 (en) 2017-09-22 2019-03-28 City Of Hope OLIGONUCLEOTIDES SPLICE INHIBITORS
US20200354452A1 (en) 2017-09-29 2020-11-12 City Of Hope Cars and bispecific antibodies for treatment of mantle cell lymphoma
WO2019067677A1 (en) 2017-09-29 2019-04-04 Cell Design Labs, Inc. METHODS OF MANUFACTURING RECEPTORS OF ANTI-CD307E ANTI-BACTERIAL CHIMERIC ANTIGENS AND USES THEREFOR
MX2020003536A (es) 2017-10-03 2020-09-14 Juno Therapeutics Inc Moleculas de union especifica a virus de papiloma humano (hpv).
US11779654B2 (en) 2017-10-04 2023-10-10 2Seventy Bio, Inc. PCSK9 endonuclease variants, compositions, and methods of use
GB2567188A (en) 2017-10-05 2019-04-10 Ft Tech Uk Ltd Fluid flow measuring device and associated method
US11052801B2 (en) 2017-10-06 2021-07-06 Harman International Industries, Incorporated Vehicle headrest with speakers and control for adaptive spatial performance
WO2019075409A1 (en) 2017-10-12 2019-04-18 The Regents Of The University Of California ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM)
KR20200064131A (ko) 2017-10-13 2020-06-05 베링거 인겔하임 인터내셔날 게엠베하 관류 배지
US20190111480A1 (en) 2017-10-17 2019-04-18 Desktop Metal, Inc. Binder jetting in additive manufacturing of inhomogeneous three-dimensional parts
US11618780B2 (en) 2017-10-20 2023-04-04 City Of Hope Composition and method for activating latent human immunodeficiency virus (HIV)
WO2019081521A1 (en) 2017-10-25 2019-05-02 Universite De Lille ACOUSTIC KNIVES
EP3700536A4 (en) 2017-10-27 2021-11-17 Shanghai Yao Yuan Biotechnology Co., Ltd. COMPOSITIONS AND METHODS FOR MODULATING THE IMMUNE RESPONSE BY ACTIVATION OF KINASE ALPHA 1 PROTEIN
WO2019087009A1 (en) 2017-11-01 2019-05-09 Mahmoud Amouzadeh Tabrizi Nanomotors for reduction of nitroarenes
US11788057B2 (en) 2017-11-01 2023-10-17 Mcmaster University 3D label-free contactless formation of cellular structures and co-cultures through diamagnetophoresis
US10304490B2 (en) 2017-11-02 2019-05-28 AcoustiX VR Inc. Acoustic holographic recording and reproduction system using meta material layers
KR20200085780A (ko) 2017-11-07 2020-07-15 더 보드 오브 리젠츠 오브 더 유니버시티 오브 텍사스 시스템 암의 치료에서 car-t 또는 car-nk 세포를 사용한 lilrb4 표적화
CA3079748A1 (en) 2017-11-09 2019-05-16 Sangamo Therapeutics, Inc. Genetic modification of cytokine inducible sh2-containing protein (cish) gene
SG11202004145QA (en) 2017-11-15 2020-06-29 Weird Science Llc Methods and compositions for non-myeloablative bone marrow reconstitution
WO2019099736A1 (en) 2017-11-15 2019-05-23 The Regents Of The University Of California Methods of treating extrachromosomal dna expressing cancers
WO2019099949A1 (en) 2017-11-17 2019-05-23 The Regents Of The University Of California Manipulation of the retinoic acid signaling pathway
NL2019937B1 (en) 2017-11-17 2019-05-24 Stichting Vu Method and system for optical force measurement
DE102017220792A1 (de) 2017-11-21 2019-05-23 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren und Vorrichtung zum Sortieren von Teilchen eines Materialstroms
US11679148B2 (en) 2017-11-24 2023-06-20 Institut National De La Santé Et De La Recherche Médicale (Inserm) Methods and compositions for treating cancers
EP3495376A1 (en) 2017-12-05 2019-06-12 ChromaCon AG Improved chromatographic process for cell culture harvest
US20210300986A1 (en) 2017-12-05 2021-09-30 The Medical Research Infrastrutrure And Health Services Fund Of The Tel Aviv Medecal Center T-cells comprising two different chimeric antigen receptors and uses thereof
BR112020009858A2 (pt) 2017-12-06 2020-11-17 Generation Bio Co. edição de genes com o uso de um dna modificado com extremidades fechadas (cedna)
EP3495811A1 (en) 2017-12-11 2019-06-12 Sensor-Kinesis Corporation Field portable handheld, recirculating surface acoustic wave and method for operating the same
JP2021506243A (ja) 2017-12-13 2021-02-22 ヤンセン バイオテツク，インコーポレーテツド Ｔ細胞受容体及びβ２ミクログロブリン発現を排除するように遺伝子改変された不死化ＣＡＲ−Ｔ細胞
EP3725092A4 (en) 2017-12-14 2021-09-22 FloDesign Sonics, Inc. DRIVE AND CONTROL UNIT FOR ACOUSTIC CONVERTER

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990005008A1 (de) * 1988-11-03 1990-05-17 Ewald Benes Verfahren und einrichtung zur separation von teilchen
US5711888A (en) * 1993-05-11 1998-01-27 Sonosep Biotech, Inc. Multilayered piezoelectric resonator for the separation of suspended particles
US20120328447A1 (en) * 2011-06-24 2012-12-27 Alstom Technology Ltd Blade of a turbomachine
US20130277317A1 (en) * 2012-04-20 2013-10-24 Flodesign Sonics Inc. Acoustophoretic enhanced system for use in tanks

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108449987A (zh) * 2015-11-24 2018-08-24 保罗·谢勒学院 通过收集x射线衍射图像以原子分辨率解析晶体的晶体结构的系统和方法
CN108449987B (zh) * 2015-11-24 2021-08-24 保罗·谢勒学院 以原子分辨率解析晶体的晶体结构的系统和方法
CN111821601A (zh) * 2020-05-27 2020-10-27 深圳艾素科技有限公司 一种电子过滤装置及其操作方法

Also Published As

Publication number Publication date
CN105939767B (zh) 2018-04-06
CA2935960C (en) 2023-01-10
US20170369865A1 (en) 2017-12-28
US10975368B2 (en) 2021-04-13
US20150191716A1 (en) 2015-07-09
US9725710B2 (en) 2017-08-08
CA2935960A1 (en) 2015-07-16
EP3092049A1 (en) 2016-11-16
WO2015105955A1 (en) 2015-07-16

Similar Documents

Publication Publication Date Title
CN105939767B (zh) 具有双声电泳腔的声电泳装置
EP3164488B1 (en) Acoustophoretic device with uniform fluid flow
US10550382B2 (en) Acoustophoretic device for angled wave particle deflection
US20220040733A1 (en) Non-planar and non-symmetrical piezoelectric crystals and reflectors
US9725690B2 (en) Fluid dynamic sonic separator
US9457302B2 (en) Acoustophoretic device with piezoelectric transducer array
US9701955B2 (en) Acoustophoretic separation technology using multi-dimensional standing waves
US10953436B2 (en) Acoustophoretic device with piezoelectric transducer array
US9512395B2 (en) Acoustophoresis device with modular components
CN105143835B (zh) 使用多维驻波的声泳分离技术
US9623348B2 (en) Reflector for an acoustophoretic device
US20170088809A1 (en) Multi-stage acoustophoresis device
US10322949B2 (en) Transducer and reflector configurations for an acoustophoretic device
CN109069954A (zh) 多站式声泳装置

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant