WO1982001072A1 - Immunoassay utilizing monoclonal high affinity igm antibodies - Google Patents

Immunoassay utilizing monoclonal high affinity igm antibodies Download PDF

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WO1982001072A1
WO1982001072A1 PCT/US1981/001270 US8101270W WO8201072A1 WO 1982001072 A1 WO1982001072 A1 WO 1982001072A1 US 8101270 W US8101270 W US 8101270W WO 8201072 A1 WO8201072 A1 WO 8201072A1
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antigen
antibodies
assay
igm
solid phase
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French (fr)
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General Hospital Massachusetts
J Wands
V Zurawski
H Schoenmaker
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General Hospital Corp
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
    • C07K16/082—Hepadnaviridae, e.g. hepatitis B virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/948—Microorganisms using viruses or cell lines
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/804—Radioisotope, e.g. radioimmunoassay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/82—Hepatitis associated antigens and antibodies
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/826—Viruses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/863—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving IgM
    • Y10S530/864—Monoclonal

Definitions

  • the present invention relates to an immunoassay system which utilizes hybridomally produced high affinity IgM antibodies.
  • immunoassay for the detection of minute amounts of substances in physiological fluids is very well known in the art.
  • the assay basically depends on the binding interaction between an antigen and an antibody therefor.
  • the antibody is usually immunoglobulin G (IgG).
  • IgG immunoglobulin G
  • Patent 4,196,265 describe continuous cell lines of genetically stable fused cell hybrids capable of producing large amounts of IgG antibodies against specific viruses, such as influenza virus, rabies, mumps, SV40, and the like. Zurawski et al, Federation Proceedings 39: 4922 (1980), disclose hybridomas producing monoclonal IgG antibodies against tetanus toxin. Frankel and Gerhard, Molecular Immunology, 16 :101-106 (1979) also disclose monoclonal antiviral hybridoma IgG antibodies described as "true bivalent antibodies", Frankel and Gerhard, at 105. The Frankel and Gerhard paper describes what are probably the prior art antibodies having the highest affinities to date.
  • the monoclonal antibodies of Frankel and Gerhard have K values (binding constants determined from Scatchard plots) in the ranges of from less than 10 5 M -1 to about 10 10 M -1 .
  • the aforementioned Zurawski et al paper describes monoclonal antibodies having K association constants in the range of 3.5-5.4X10 8 M -1 .
  • IgM antibodies consist of 5 units each the approximate size of IgG joined by a so-called J (joining) chain.
  • the IgM molecule has a valency of 10, although quantitative antigen binding measurements often detect only 5.
  • IgM's are extremely sensitive to reducing agents, they self-aggregate readily and precipitate out of solution, and have been found to bind in a non-specific manner. These reasons, among others, have heretofore prevented their extensive use. Because of the polyvalency of IgM antibodies, various suggestions have been made in the art concerning the theoretical binding affinity of these antibodies towards antigens.
  • Metzger has suggested that it would be "intuitively obvious” that an antibody with multiple binding sites would bind more firmly than a univalent or bivalent antibody to a multivalent antigen. Metzger however qualifies this statement recognizing that the energetics of binding would be highly dependent on the number and topology of antigenic determinants, the distribution of combining sites in space and the balance between the free energy of binding released by combining site/determinant interactions and high free energy required to maximize the number of multiple flexible conformations existing in such large molecules. These qualifications are even more stringent when it comes to cross linking of translationally independent determinants such as in agglutination.
  • IgM antibodies are nultivalent, the binding affinities observed for them have, prior to this invention, always been substantially less than the binding affinities for IgG antibodies. It is for this reason that IgM's have not routinely been utilized for immunoassays, other than in certain specific circumstances, infra.
  • rheumatoid factor rheumatoid factor
  • RF's are IgM antibodies directed towards antigenic determinants present on autologous immunoglobulin Lurhuma et al, Clinical and Experimental immunology, 25:212-226 (1976), describe the use of RF as an agglutinant for IgG coated particles, and an inhibition of aglutination effect which occurs in the presence of antibody-antigen complexes.
  • Masson et al U. S. Patent 4,062,935 describe a similar system to that of Lurhuma.
  • Monoclonal RF to human IgG has been used by Eisenberg, Immunochemistry, 13 : 355-359 (1976) to demonstrate binding to aggregated IgG.
  • the binding of monoclonal RF to aggregated IgG is increased by a factor of greater than 10 6 (as determined by radioimmunoassay) over the binding of RF to nonaggregated, monomeric, IgG.
  • IgM antibodies whether polyclonal or monoclonal has been restricted to assaying for antibody-antigen complexes, aggregated IgG's, or, by an inhibition of agglutination technique, to antigen-coated latex particles. Because of the poor binding affinity of IgM's as well as their self-aggregating tendency, non-specific interactions and sensitivity to reducing agents, it has generally been assumed in the art that IgM antibodies were not useful for highly sensitive immunoassay techniques.
  • An immunoassay system utilizing an antibody to assay for a viral antigen, wherein said antibody is a hybridomally produced monoclonal high affinity IgM.
  • FIGURE 1 shows the purification of hepatitis B surface antigen (HBsAg) of the adw subtype from serum, with a hybridomally produced monoclonal IgM anti-HBs affinity column.
  • HBsAg hepatitis B surface antigen
  • the elution profile of protein and HBsAg activity is measured by a solid phase radioimmunoassay from a cyanogen bromide activated Sepharose 6B control column.
  • FIGURE 2 shows a similar purification to that of FIGURE 1 wherein the elution profile is from a control column prepared with 30 mg of mouse serum.
  • FIGURES 3 and 4 represent affinity columns prepared with 28 and 65 mg of ascites fluid protein from clones 5D3 and 2F11. It should be noted that the monoclonal hybridomally produced IgM anti-HBs coupled to Sepharose 6B bound HBsAg, and substantial antigenic activity and protein were recovered following elution with glycine-HCl pH2.6. Negative serum controls for HBsAg activity where 426 106 cpm and positive serum controls were 12,462 321 cpm.
  • the specificity relates to the lack of cross reactivity or lack of interference in the antigen-antibody reaction by other substances present in the reaction mixture.
  • Hybridomally produced antibodies in general, including IgG's exhibit an extraordinary specificity since they are directed against a single determinant. However because they are directed against a single determinant, they bind to fewer sites on an antigen than classically (polyclonally) produced antibodies. This fact had been reflected in an existing fear in the prior art that the extraordinary specificity of monoclonally produced antibodies, would be a detriment to their utilization in sensitive clinically useful immunoassays.
  • hybridomally produced IgM's can detect less antigen than the conventionally produced antibodies, despite their extraodinary specificity.
  • the hybridomally produced IgM antibodies for specific viral antigens utilized in the present invention can be obtained, for example, through the use of cell fusion techniques first described by Kohler and Milstein (Nature, 255:495 (1975)). Lymphocytes from an aninal immunized with the antigen of choice are fused to a continuously proliferating myeloma cell. Subsequently, hybrid cells are selected in hypoxanthine-aminopterin-thymidine (HAT) medium (Littlefield, Science, 145:709 (1964)).
  • HAT hypoxanthine-aminopterin-thymidine
  • IgM antibody of choice Those which produce the IgM antibody of choice are cloned and propagated indefinitely in tissue culture or in syngeneic or immunocompromised animals, or in athymic animals, for example nude mice.
  • Such clones are the progeny of single cell produced or monodisperse IgM antibodies, i.e., monoclonal IgM antibodies, which is directed to an individual determinant or an antigen. This determinant is expressed more than once on the surface of each individual viral particle or frequent thereof. Further details on the preparation of monoclonal IgM antibodies can be found in the aforementioned Wands and Zurawski U. S. Patent Application.
  • the hybridomally produced high affinity IgM antibodies of the present invention are those having affinity binding constants greater than 10 9 M-1, preferably greater than 10 10 M -1 , most preferably greater than 10 11 M -1 . In order to produce such high binding antibodies it is necessary to use a viral antigen or a fragment thereof.
  • viral antigens which may be determined in the present invention are virus particles, fragments of virus particles such as whole coats, or individual molecular components of the coat, e.g., polypeptides or lipid or carbohydrate complexes.
  • viral antigens are hepatitis B surface antigens, hepatitis Be antigen, hepatitis B core antigens, hepatitis A virus and isolated antigens thereof, herpes viruses I and II, herpes virus Zoster, cytomegalovirus, Epstein-Barr virus, papova viruses such as BK or JC virus, measles virus, rubella virus, influenza or para influenza virus, or any other viruses known or suspected of being etiologic in pathogenesis or animals or plants.
  • the high affinity hybridomally produced IgM antibodies of the present invention can be used in any of the array of available immunoassay techniques which utilize the binding interaction between an antibody and an antigen, and the present invention is not limited to any of these techniques.
  • the most common of these is radioimmunoassay (RIA).
  • RIA is a well known technique and will not be described in detail here. For particulars, reference is made to Chard, "An Introduction to Radioimmunoassy and Related Techniques", North-Holland Publishing Company, 1978. Any of the many variations of RIA can be used, such as homogeneous phase RIA, or heterogeneous or solid phase RIA, single antibody methods or “double” antibody methods, and direct (forward) or reverse sandwich assays.
  • solid phase systems wherein the antibody (IgG or IgM) is covalently coupled to an insoluble support so that both the antibody and the bound complex after incubation can be readily separated from the soluble free fraction.
  • solid phase supports include particles of dextran, cellulose, continuous surfaces such as polystyrene or polypropylene discs, walls of plastic tubes, glass discs, glass particles, and the like. Particulate solid phases are widely used for a variety of different assays and are included in the present invention.
  • Antibodies are attached to the particles by any of a number of techniques designed to yield a non- reversible covalent or non-covalent link between protein and particle, for example directly or by cyanogen bromide activation.
  • the assay tube is set up with sample or standard, tracer and the appropriate amount of solid phase bound antibody, plus a detergent to prevent aggregation of the particles and non-specific absorption of the tracer. After an incubation period during which the tubes are continuously mixed, the solid phase is sedimented by centrifugation; the supernatant is removed and the solid phase subject to two or more washes with buffer in order to remove free tracer trapped within and between the particles. The counts on the solid phase (bound fraction) are then measured. Immunoradiometric assays, Chards, supra at 423, can also be used. When a second antibody radioimmunoassay system is used, the second antibody may be IgM or may be IgG.
  • Another immunoassay technique useful with the IgM antibodies of the present invention is enzyme immunoassay. This technique is also well known to the art and reference is made to Schuurs and VanWeemen, Clinica Chimica Acta, 81:1-40 (1977). In this technique, enzymes are applied as labels on antigen or antibodies for identification and localization of the immunoreactants. Any method in which the extent of binding of enzyme-labeled antigen, or enzyme labeled-antibody to its immunoreactant is measured, is included in this invention.
  • Enzyme immunoassays can be classified as homogeneous or heterogeneous, depending on whether the labeled reagent behaves differently or identically whether or not it is bound to specific counterparts in the immunoreaction, and which therefore may or may not require physical separation of the reactants into two fractions.
  • the variety of enzymes used, methods of linking enzymes to the immunological components, purification of the conjugates, as well as various assay principles and methods are well described in the aforementioned Schuurs and VanWeemen article.
  • any enzyme immunoassay which has used IgG antibodies in the past can be used with the high affinity IgM antibodies of the present invention.
  • Another immunoassay method useful in the present invention is the latex agglutination method.
  • latex particles are coated with viral antigen and incubated with high affinity hybridomally produced IgM antibodies. Inhibition of agglutination will occur when a sample of physiological fluid containing the viral antigen is incubated with this mixture. The inhibition of agglutination can either be followed with a counter or by recently developed infrared absorption techniques.
  • An alternative is to coat the latex particles with the hybridomally produced IgM antibodies. Incubation of these coated particles with physiological fluid comprising viral antigen will cause agglutination.
  • animal cells such as red blood cells can of course be used. In this case, the technique becomes a variation of the well known hemagglutination technique used with IgG antibodies and red blood cells.
  • immunoassay techniques are those using other labeling techniques such as: fluorescent dyes (Aalbeses, R.C. Clin. Chim. Acta 48:109-111 (1973); electron-dense compounds (ferritin) Singer, S.J. et al, J. Biophys. Biochem. Cyto; 9: 519-537 (1961); protein - bacteriophage conjugates, (Haimovich, J. et al, Biochim. Biophys. Acta 207: 115-124 (1970); or stable free radicals (Bastiani, R. J., et al, Am. J. Med. Technol. 39 : 211-216 (1973).
  • the monoclonal IgM antibodies of the present invention it is also possible to use the same in purification methodologies.
  • the most common of these comprises binding the hybridomal IgM antibody on a cellulose based solid phase support, adding a complex mixture containing the viral antigen recognizable by the antibody, thereby binding said antigen to said antibody, washing the column extensively with buffers and eluting the viral antigen by changing the column conditions, such as the ionic strength, the pH or the like.
  • This example describes the use of IgM monoclonal antibodies to hepatitis B surface antigen (HBsAg) in several solid phase radioimmunoassays.
  • Two general types of radioimmunoassays are defined. One assay is performed in the reverse direction. Under these experimental conditions serum containing the hepatitis antigen is first incubated with 125 I -monoclonal IgG or
  • IgM antihepatitis B antigen, or heterologous antihepatitis B prepared by conventional immunizations followed by a second incubation with monoclonal IgM anti HBs or heterologous anti-HBS coupled to a solid phase, for example polystyrene beads.
  • the other assay is performed in the forward direction.
  • monoclonal IgM anti-HBs or heterologous anti-HBs coupled to a solid phase is first incubated with serum containing. HBsAg.
  • the second incubation therefore, is comprised of 125 I -monoclonal IgG or IgM anti-HBs or heterologous anti-HBs.
  • the sensitivity and specificity of the monoclonal IgM and IgG. anti-HBs sandwich assays are compared directly to conventional assays either alone or in combination with anti-HBs prepared by standard animal immunizations.
  • Monoclonal IgM and IgG antibodies with specificity for HBsAg were obtained according to the procedures described by Wands and Zurawski copending U. S. Patent Application Number 086,947, October 22, 1979.
  • One quarter inch plastic beads were obtained commercially; identical beads coated with conventionally prepared antibodies to HBsAg (AUSRIA II) were also obtained commercially (Abbott Laboratories, N. Chicago, IL.)
  • Plastic beads were coated with IgM hybridomal anti-HBs as follows: Ascites fluid derived from malignant growth in BALB/mice of double cloned hybridoma cell lines which produce IgM anti-HBs antibodies was diluted 1:1000 to 1:5000 in phosphate buffered saline. The beads were incubated for 16 hours at room temperature and washed X3 with distilled water: the beads coated with IgM anti-HBs were then ready for use in the RIAs.
  • the HBsAg was radiolabeled with Na 125 I using the chloramine T method of Hunter and Greenwood, Nature 194:495 (1962) or Hunter and Bolton, Biochemical Journal, 133: 529-(1973) ; specific activity ranged from 0.1 to 0.4 ⁇ Ci/ ⁇ g and incorporation of 125 I was 20-26 percent.
  • the monoclonal IgG anti-HBs antibodies were also labeled according to the above method; specific activity ranged from 0.1 to 15 ⁇ Ci/ ⁇ g and incorporation of radiolabel varied from 20 to 50 percent.
  • Assays were carried out with various combinations of coated beads and labeled antibodies or antigen to establish the efficacy of the technology.
  • individual coated beads were washed X3 with distilled water then mixed with dilutions of patient sera, HBsAg standards, radiolabeled HBsAg or anti-HBs as described in the results.
  • the beads were reincubated with radiolabeled reagents as necessary, washed, collected and counted in a Packard gamma well spectrometer. Incubation time varied between 1 and 16 hours as described below.
  • Table 1 represents the kinetic binding data obtained when plastic beads coated with monoclonal IgM anti-HBs (designated 5D3 and 1F8) were incubated with labeled 125 I-HBsAg.
  • AUSRIA II IgG anti-HBs antibodies Moreover , a greater percent of total 125 I-HBsAg added is bound at 24 hours by the IgM antibodies .
  • a simple substitution was made of the 5D3 coated beads for the AUSRIA II coated beads and the sens itivity of the two assays was directly compared as shown in Table II . in this experiment dilutions of 2 patients' sera known to contain HBsAg were incubated with 5D3 coated beads or AUSRIA II beads for 16 hours. The beads were washed X3 with distilled water. 200 ⁇ l (150,000 cpm) of AUSRIA II 125 I-guinea pig antibodies (anti-HBs) prepared by conventional immunization were then added to both sets of beads for 4 hours. The beads were washed again and counted.
  • +S/N is the signal/noise calculated by dividing the mean cpm in test sample by mean cpm of the negative controls.
  • a reverse sandwich assay using 1) a total monoclonal system or 2) a combination of IgM and IgG monoclonal anti-HBs antibodies with the AUSRIA II reagents was then prepared.
  • the incubation conditions were identical for all assays and therefore, the results are directly comparable.
  • HBsAg standard was first incubated at 10 ng/ml with 125 I-anti-HBs (guinea pig antisera AUSRIA II) or monoclonal IgG 125 I-anti-HBs of varying specific activities (0.1 to 16 ⁇ Ci/ ⁇ g) for 1 hour at 45°C. Beads were washed and counted. The results are depicted in Table III. +
  • AUSRIA II run in the forward direction (e.g. , beads first incubated for 1 hr at 45°C with HBs-Ag standard, then washed, and 200 ⁇ l 125 l--anti--HBs (guinea pig) added for an additional 1 hr period) yields a S/N of 20.6.
  • the sensitivity of the RIAs has been improved under all experimental conditions : further the total monoclonal sandwich assay was very sens itive for detection of HBsAg.
  • the AUSRIA II assay was also substantially improved by the use of 125 I-IgG monoclonal anti-HBs or by substituting the 5D3 coated beads for their beads in the reverse direction assay.
  • HBsAg pos itive dilutions 200 ⁇ l were incubated with 5D3 or AUSRIA II beads for 16 hours at room temperature .
  • the beads were washed X3 with distilled water .
  • the second incubation covered 4 hours at room temperature with either monoclonal 12 5 I-anti-HBs (AUSRIA II) probes.
  • Tables V and VI demonstrate that very sensitive RIA's performed in the forward and reverse directions were developed using either a total monoclonal IgG-IgM or IgM-IgM system or a combination assay of monoclonal antibodies with AUSRIA II reagents.
  • EXAMPLE II This example describes the use of monoclonal IgM anti-HBs in the biopurification of HBsAg from serum.
  • FIGS 1-4 demonstrate that HBsAg (adw subtype) present in serum, bound to the monoclonal IgM anti-HBs affinity columns prepared from ascites fluid derived from clones 5D3 and 2F11. It is noteworthy that previous studies have established that monoclonal IgM anti-HBs from 2F11 and 5D3 recognized the adw subtype. Subsequently, the HBsAg could be recovered from the columns in ⁇ g amounts by elution with glycine HC1 buffer pH 2.6. In contrast, there was no significant binding of HBsAg to the Sepharose 6B column or to Sepharose 6B coupled to mouse serum. This study illustrates the use of monoclonal IgM in the purification and characterization of antigens.
  • EXAMPLE III This example describes the use of monoclonal IgM antibodies in the detection of HBsAg coated to red blood cells.
  • the experiments demonstrate that is also feasible to detect free HBsAg in serum by first coating IgM anti-HBs to red blood cells and incubating such cells with HBsAg-containing serum to produce a hemagglutination reaction.
  • Human O-negative red blood cells coated with HBsAg subtypes adw and ayw were purchased commercially. 25 ⁇ l of buffer was placed in microtiter plates followed by serial 2-fold dilution of culture supernatants and ascites fluid from three IgM anti-HBs producing hybridoma cell lines: plates were incubated at 37°C for 30 minutes. Following this incubation 25 ⁇ l of a 0.1% solution of HBsAg coated cells were added to each well. The reaction mixture was allowed to incubate at 37°C for 30 minutes, plates were centrifuged at 300 x g for 2 minutes and placed at a 45° angle. After 10 minutes the plates were assessed for the presence or absence of hemagglutination.
  • IgM anti-HBs are exceptionally good agglutinating antibodies.
  • 25 ⁇ l of cell supernatant from line 5D3 gave hemagglutination titers of 1:2.1xl0 9 and 1:3.2x10 7 for HBsAg (ayw and adw) coated red blood cells respectively.
  • the agglutinating capacity was amplified in the ascites fluid since extraordinary high titers of 1:2.2x10 12 and 1:2.7x10 11 were noted for the same HBsAg coated cells.
  • monoclonal IgM anti-HBs may be employed as a probe for detection of anti-HBs in serum by an inhibition radioimmunoassay.
  • Table VIII illustrates the results of the experiment. increasing serum dilution. This assay, therefore, measures inhibition of antigen binding by serum containing anti-HBs and is a sensitive method for detection of such antibodies.
  • This binding results from the fact that antibody to hepatitis B surface antigen (anti-HBs) in test serum complexes first to HBsAg, and therefore was unavailable for binding with the radiolabeled probe ( 125 I-1C7) at both the undiluted and 1:10 serum dilution.
  • the added HBsAg (5 ng/ml) now becomes available for binding to 125 I-1C7 anti-HBs.
  • HBsAg is now in excess of anti-HBs concentrations in serum at the higher dilutions and is detectable as shown by increasing cpm bound to the 5D3 coated beads in the RIA.
  • This assay therefore measures inhibition of antigen binding (HBsAg) by serum containing anti-HBs and is a sensitive method for detection of such antibodies.

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PCT/US1981/001270 1980-09-19 1981-09-21 Immunoassay utilizing monoclonal high affinity igm antibodies Ceased WO1982001072A1 (en)

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AT81902711T ATE20553T1 (de) 1980-09-19 1981-09-21 Immunobiologischer test mit verwendung monoklonaler igm-antikoerper hoher affinitaet.
DE8181902711T DE3174878D1 (en) 1980-09-19 1981-09-21 Immunoassay utilizing monoclonal high affinity igm antibodies

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