RU2487166C2 - Изолированные полипептиды, кодирующие их полинуклеотиды, экспрессирующие их трансгенные растения и способы их использования - Google Patents

Изолированные полипептиды, кодирующие их полинуклеотиды, экспрессирующие их трансгенные растения и способы их использования Download PDF

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RU2487166C2
RU2487166C2 RU2011113420/10A RU2011113420A RU2487166C2 RU 2487166 C2 RU2487166 C2 RU 2487166C2 RU 2011113420/10 A RU2011113420/10 A RU 2011113420/10A RU 2011113420 A RU2011113420 A RU 2011113420A RU 2487166 C2 RU2487166 C2 RU 2487166C2
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Родриго ЙЕЛИН
Ави ШОШАН
Евгения ГОЛД
Шарон АЯЛ
Хагай КАРЧИ
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Abstract

Изобретение относится к области биохимии. Представлена нуклеиновокислотная конструкция для экспрессии в клетке растения для повышения эффективности использования азота растением, содержащая нуклеиновокислотную последовательность, по меньшей мере, на 85% идентичную нуклеотидной последовательности, соответствующей SEQ ID NO: 70, и промоторную последовательность, способную направлять транскрипцию нуклеиновокислотной последовательности в клетке-хозяине. Представлен изолированный полипептид для экспрессии в клетке растения для повышения эффективности использования азота растением, содержащий аминокислотную последовательность, по меньшей мере, на 85% гомологичную аминокислотной последовательности, соответствующей SEQ ID NO: 1186. Также представлена растительная клетка для получения зрелого растения, обладающего повышенной толерантностью к стрессовым условиям посредством более эффективного использования азота растением. Раскрыты способ повышения толерантности растения к стрессовым условиям, способ увеличения биомассы, жизненности и/или урожая растения, а также способ повышения эффективности использования и/или поглощения удобрений растением. Указанные способы включают обеспечение экспрессирования в клетках растения экзогенного полинуклеотида, кодирующего полипептид, имеющий аминокислотную последовательность, по меньшей мере, на 85% гомологичную к аминокислотной последовательности, соответствующей SEQ ID NO: 1186. Изобретение позволяет повысить эффективность использования азота растением, посредством этого повышая толерантность растения к стрессовым условиям. 6 н. и 15 з.п. ф-лы, 10 ил., 50 табл., 12 пр.

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Claims (21)

1. Нуклеиновокислотная конструкция для экспрессии в клетке растения для повышения эффективности использования азота растением, содержащая нуклеиновокислотную последовательность, по меньшей мере, на 85% идентичную нуклеотидной последовательности, соответствующей SEQ ID NO: 70, и промоторную последовательность, способную направлять транскрипцию нуклеиновокислотной последовательности в клетке-хозяине.
2. Нуклеиновокислотная конструкция по п.1, где указанной клеткой-хозяином является растительная клетка.
3. Изолированный полипептид для экспрессии в клетке растения для повышения эффективности использования азота растением, содержащий аминокислотную последовательность, по меньшей мере, на 85% гомологичную аминокислотной последовательности, соответствующей SEQ ID NO: 1186.
4. Изолированный полипептид по п.3, где указанная аминокислотная последовательность соответствует SEQ ID NO: 1186.
5. Растительная клетка для получения зрелого растения, обладающего повышенной толерантностью к стрессовым условиям посредством более эффективного использования азота растением, содержащая экзогенный полинуклеотид, который содержит нуклеиновокислотную последовательность, кодирующую полипептид, имеющий аминокислотную последовательность, по меньшей мере, на 85% гомологичную аминокислотной последовательности, соответствующей SEQ ID NO: 1186.
6. Растительная клетка по п.5, где указанная растительная клетка образует часть растения.
7. Способ повышения толерантности растения к стрессовым условиям, включающий обеспечение экспрессирования в клетках растения экзогенного полинуклеотида, кодирующего полипептид, имеющий аминокислотную последовательность, по меньшей мере, на 85% гомологичную к аминокислотной последовательности, соответствующей SEQ ID NO: 1186, где экспрессия аминокислотной последовательности в клетках растения обеспечивает эффективность использования азота растением, повышая посредством этого толерантность растения к стрессовым условиям.
8. Способ по п.7, дополнительно включающий выращивание растения в стрессовых условиях.
9. Способ по п.8, где стрессовое условие является абиотическим стрессом.
10. Способ по п.9, где указанный абиотический стресс является выбранным из группы, состоящей из засоленности, засухи, низкой температуры, высокой температуры, токсичности тяжелых металлов, анаэробиоза, осмотизма и дефицита питательных веществ.
11. Способ увеличения биомассы, жизненности и/или урожая растения, включающий обеспечение экспрессирования в клетках растения экзогенного полинуклеотида, кодирующего полипептид, имеющий аминокислотную последовательность, по меньшей мере, на 85% гомологичную к аминокислотной последовательности, соответствующей SEQ ID NO: 1186, посредством этого увеличивая биомассу, жизненность и/или урожай растения.
12. Способ повышения эффективности использования и/или поглощения удобрений растением, включающий обеспечение экспрессирования в клетках растения экзогенного полинуклеотида, кодирующего полипептид, имеющий аминокислотную последовательность, по меньшей мере, на 85% гомологичную к аминокислотной последовательности, соответствующей SEQ ID NO: 1186, посредством этого повышая эффективность использования и/или поглощения удобрений растением.
13. Способ по п.12, дополнительно включающий выращивание растения в условиях ограниченности удобрений.
14. Способ по любому из пп.7-13, где указанное растение является двусемядольным растением.
15. Способ по любому из пп.7-13, где указанное растение является односемядольным растением.
16. Способ по любому из пп.7-13, где указанный полинуклеотид включает нуклеиновокислотную последовательность, соответствующую SEQ ID NO: 70.
17. Способ по любому из пп.7-13, где указанная аминокислотная последовательность соответствует SEQ ID NO: 1186.
18. Способ по любому из пп.7-13, где указанное экспрессирование осуществляют путем:
(a) трансформирования клетки указанного растения указанным экзогенным полинуклеотидом;
(b) получения зрелого растения из указанной клетки и
(c) культивирования указанного зрелого растения в условиях, пригодных для экспрессирования указанного экзогенного полинуклеотида в пределах указанного зрелого растения.
19. Способ по п.18, где указанные условия являются условиями абиотического стресса.
20. Способ по п.18, где указанные условия являются условиями дефицита удобрений.
21. Способ по п.18, где указанное трансформирование осуществляют введением в указанную растительную клетку нуклеиновокислотной конструкции, включающей указанный экзогенный полинуклеотид и, по меньшей мере, один промотор, способный направлять транскрипцию указанного экзогенного полинуклеотида в указанной растительной клетке.
RU2011113420/10A 2005-10-24 2006-10-24 Изолированные полипептиды, кодирующие их полинуклеотиды, экспрессирующие их трансгенные растения и способы их использования RU2487166C2 (ru)

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