JP6328934B2 - 非侵襲性出生前親子鑑定法 - Google Patents
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Families Citing this family (178)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070027636A1 (en) * | 2005-07-29 | 2007-02-01 | Matthew Rabinowitz | System and method for using genetic, phentoypic and clinical data to make predictions for clinical or lifestyle decisions |
| US8532930B2 (en) * | 2005-11-26 | 2013-09-10 | Natera, Inc. | Method for determining the number of copies of a chromosome in the genome of a target individual using genetic data from genetically related individuals |
| US20070178501A1 (en) * | 2005-12-06 | 2007-08-02 | Matthew Rabinowitz | System and method for integrating and validating genotypic, phenotypic and medical information into a database according to a standardized ontology |
| US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
| US8515679B2 (en) * | 2005-12-06 | 2013-08-20 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US10081839B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US11111543B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US10083273B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US11111544B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US20110033862A1 (en) * | 2008-02-19 | 2011-02-10 | Gene Security Network, Inc. | Methods for cell genotyping |
| WO2009146335A1 (en) * | 2008-05-27 | 2009-12-03 | Gene Security Network, Inc. | Methods for embryo characterization and comparison |
| CA3116156C (en) | 2008-08-04 | 2023-08-08 | Natera, Inc. | Methods for allele calling and ploidy calling |
| WO2011041485A1 (en) | 2009-09-30 | 2011-04-07 | Gene Security Network, Inc. | Methods for non-invasive prenatal ploidy calling |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11332793B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| EP2854057B1 (en) | 2010-05-18 | 2018-03-07 | Natera, Inc. | Methods for non-invasive pre-natal ploidy calling |
| US11339429B2 (en) | 2010-05-18 | 2022-05-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11332785B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US12221653B2 (en) | 2010-05-18 | 2025-02-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US12152275B2 (en) | 2010-05-18 | 2024-11-26 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US20190010543A1 (en) * | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| US11326208B2 (en) | 2010-05-18 | 2022-05-10 | Natera, Inc. | Methods for nested PCR amplification of cell-free DNA |
| US10533223B2 (en) | 2010-08-06 | 2020-01-14 | Ariosa Diagnostics, Inc. | Detection of target nucleic acids using hybridization |
| US8700338B2 (en) | 2011-01-25 | 2014-04-15 | Ariosa Diagnosis, Inc. | Risk calculation for evaluation of fetal aneuploidy |
| US11203786B2 (en) | 2010-08-06 | 2021-12-21 | Ariosa Diagnostics, Inc. | Detection of target nucleic acids using hybridization |
| US20120034603A1 (en) | 2010-08-06 | 2012-02-09 | Tandem Diagnostics, Inc. | Ligation-based detection of genetic variants |
| US20130040375A1 (en) | 2011-08-08 | 2013-02-14 | Tandem Diagnotics, Inc. | Assay systems for genetic analysis |
| US20140342940A1 (en) | 2011-01-25 | 2014-11-20 | Ariosa Diagnostics, Inc. | Detection of Target Nucleic Acids using Hybridization |
| US20130261003A1 (en) | 2010-08-06 | 2013-10-03 | Ariosa Diagnostics, In. | Ligation-based detection of genetic variants |
| CA2821906C (en) | 2010-12-22 | 2020-08-25 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| US10131947B2 (en) | 2011-01-25 | 2018-11-20 | Ariosa Diagnostics, Inc. | Noninvasive detection of fetal aneuploidy in egg donor pregnancies |
| JP6153874B2 (ja) | 2011-02-09 | 2017-06-28 | ナテラ， インコーポレイテッド | 非侵襲的出生前倍数性呼び出しのための方法 |
| CN110016499B (zh) | 2011-04-15 | 2023-11-14 | 约翰·霍普金斯大学 | 安全测序系统 |
| US20140235474A1 (en) | 2011-06-24 | 2014-08-21 | Sequenom, Inc. | Methods and processes for non invasive assessment of a genetic variation |
| US10196681B2 (en) | 2011-10-06 | 2019-02-05 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9984198B2 (en) | 2011-10-06 | 2018-05-29 | Sequenom, Inc. | Reducing sequence read count error in assessment of complex genetic variations |
| US10424394B2 (en) | 2011-10-06 | 2019-09-24 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9367663B2 (en) | 2011-10-06 | 2016-06-14 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US20140242588A1 (en) | 2011-10-06 | 2014-08-28 | Sequenom, Inc | Methods and processes for non-invasive assessment of genetic variations |
| US8688388B2 (en) | 2011-10-11 | 2014-04-01 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| SG10201510189WA (en) | 2011-10-19 | 2016-01-28 | Nugen Technologies Inc | Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing |
| HUE059847T2 (hu) | 2012-01-20 | 2023-01-28 | Sequenom Inc | Kísérleti körülményeket fakturáló diagnosztikai folyamatok |
| CN104093890B (zh) | 2012-01-26 | 2016-04-20 | 纽亘技术公司 | 用于靶向核酸序列富集和高效文库产生的组合物和方法 |
| GB2513024B (en) * | 2012-02-27 | 2016-08-31 | Cellular Res Inc | A clonal amplification method |
| ES2905448T3 (es) * | 2012-05-10 | 2022-04-08 | Massachusetts Gen Hospital | Métodos para determinar una secuencia nucleotídica |
| US10504613B2 (en) | 2012-12-20 | 2019-12-10 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9920361B2 (en) | 2012-05-21 | 2018-03-20 | Sequenom, Inc. | Methods and compositions for analyzing nucleic acid |
| WO2013177581A2 (en) * | 2012-05-24 | 2013-11-28 | University Of Washington Through Its Center For Commercialization | Whole genome sequencing of a human fetus |
| US9957549B2 (en) | 2012-06-18 | 2018-05-01 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US10497461B2 (en) | 2012-06-22 | 2019-12-03 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| KR101890466B1 (ko) * | 2012-07-24 | 2018-08-21 | 내테라, 인코포레이티드 | 고도의 다중 ｐｃｒ 방법 및 조성물 |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| US11913065B2 (en) | 2012-09-04 | 2024-02-27 | Guardent Health, Inc. | Systems and methods to detect rare mutations and copy number variation |
| ES2769241T5 (es) | 2012-09-04 | 2023-05-30 | Guardant Health Inc | Sistemas y métodos para detectar variación en el número de copias |
| US10876152B2 (en) | 2012-09-04 | 2020-12-29 | Guardant Health, Inc. | Systems and methods to detect rare mutations and copy number variation |
| US10482994B2 (en) | 2012-10-04 | 2019-11-19 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| PL2912468T3 (pl) | 2012-10-29 | 2019-04-30 | Univ Johns Hopkins | Test papanicolaou pod kątem raka jajnika i endometrium |
| US20130309666A1 (en) | 2013-01-25 | 2013-11-21 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9235808B2 (en) | 2013-03-14 | 2016-01-12 | International Business Machines Corporation | Evaluation of predictions in the absence of a known ground truth |
| CN105518151B (zh) * | 2013-03-15 | 2021-05-25 | 莱兰斯坦福初级大学评议会 | 循环核酸肿瘤标志物的鉴别和用途 |
| EP2971130A4 (en) | 2013-03-15 | 2016-10-05 | Nugen Technologies Inc | SEQUENTIAL SEQUENCING |
| WO2014165596A1 (en) | 2013-04-03 | 2014-10-09 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| WO2014190286A2 (en) | 2013-05-24 | 2014-11-27 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| DK3011051T3 (en) | 2013-06-21 | 2019-04-23 | Sequenom Inc | Method for non-invasive evaluation of genetic variations |
| ES2857908T3 (es) | 2013-08-28 | 2021-09-29 | Becton Dickinson Co | Análisis masivamente paralelo de células individuales |
| US10577655B2 (en) | 2013-09-27 | 2020-03-03 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| US10262755B2 (en) | 2014-04-21 | 2019-04-16 | Natera, Inc. | Detecting cancer mutations and aneuploidy in chromosomal segments |
| US9499870B2 (en) | 2013-09-27 | 2016-11-22 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| KR102514024B1 (ko) | 2013-10-04 | 2023-03-23 | 시쿼넘, 인코포레이티드 | 유전적 변이의 비침습 평가를 위한 방법 및 프로세스 |
| CN111863131A (zh) | 2013-10-07 | 2020-10-30 | 塞昆纳姆股份有限公司 | 用于非侵入性评估染色体改变的方法和过程 |
| CN105849264B (zh) | 2013-11-13 | 2019-09-27 | 纽亘技术公司 | 用于鉴别重复测序读数的组合物和方法 |
| WO2015112974A1 (en) | 2014-01-27 | 2015-07-30 | The General Hospital Corporation | Methods of preparing nucleic acids for sequencing |
| US9745614B2 (en) | 2014-02-28 | 2017-08-29 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
| GB2524948A (en) * | 2014-03-07 | 2015-10-14 | Oxford Gene Technology Operations Ltd | Detecting Increase or Decrease in the Amount of a Nucleic Acid having a Sequence of Interest |
| US12492429B2 (en) | 2014-04-21 | 2025-12-09 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| AU2015249846B2 (en) | 2014-04-21 | 2021-07-22 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| US20180173845A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| US10119167B2 (en) * | 2014-07-18 | 2018-11-06 | Illumina, Inc. | Non-invasive prenatal diagnosis of fetal genetic condition using cellular DNA and cell free DNA |
| ES3047391T3 (en) | 2014-07-30 | 2025-12-03 | Sequenom Inc | Methods and processes for non-invasive assessment of genetic variations |
| JPWO2016038929A1 (ja) * | 2014-09-11 | 2017-04-27 | 富士フイルム株式会社 | 胎児染色体の異数性の有無を検出する方法 |
| CN107075730A (zh) | 2014-09-12 | 2017-08-18 | 利兰·斯坦福青年大学托管委员会 | 循环核酸的鉴定及用途 |
| WO2016049878A1 (zh) * | 2014-09-30 | 2016-04-07 | 深圳华大基因科技有限公司 | 一种基于snp分型的亲子鉴定方法及应用 |
| EP3224382A1 (en) * | 2014-11-24 | 2017-10-04 | Shaare Zedek Medical Center | Fetal haplotype identification |
| US20170351805A1 (en) * | 2014-12-26 | 2017-12-07 | National University Corporation Tohoku University | Method for determining genotype of particular gene locus group or individual gene locus, determination computer system and determination program |
| ES2836802T3 (es) | 2015-02-27 | 2021-06-28 | Becton Dickinson Co | Códigos de barras moleculares espacialmente direccionables |
| EP4180535A1 (en) | 2015-03-30 | 2023-05-17 | Becton, Dickinson and Company | Methods and compositions for combinatorial barcoding |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| EP3294906B1 (en) | 2015-05-11 | 2024-07-10 | Natera, Inc. | Methods for determining ploidy |
| CN104946773B (zh) * | 2015-07-06 | 2018-04-13 | 厦门万基生物科技有限公司 | 一种利用snp进行产前亲权关系判定的方法 |
| KR101817785B1 (ko) * | 2015-08-06 | 2018-01-11 | 이원다이애그노믹스(주) | 다양한 플랫폼에서 태아의 성별과 성염색체 이상을 구분할 수 있는 새로운 방법 |
| WO2017027653A1 (en) | 2015-08-11 | 2017-02-16 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| ES2745694T3 (es) | 2015-09-11 | 2020-03-03 | Cellular Res Inc | Métodos y composiciones para normalización de biblioteca de ácido nucleico |
| WO2017082034A1 (ja) * | 2015-11-10 | 2017-05-18 | 富士フイルム株式会社 | 細胞間または細胞群間の同一人かどうか、他人かどうか、親子かどうか、または血縁関係かどうかの判定方法 |
| WO2017181202A2 (en) | 2016-04-15 | 2017-10-19 | Natera, Inc. | Methods for lung cancer detection |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US11708574B2 (en) | 2016-06-10 | 2023-07-25 | Myriad Women's Health, Inc. | Nucleic acid sequencing adapters and uses thereof |
| EP3491560A1 (en) | 2016-07-27 | 2019-06-05 | Sequenom, Inc. | Genetic copy number alteration classifications |
| JP6997772B2 (ja) | 2016-09-15 | 2022-01-18 | アーチャーディーエックス， エルエルシー | 核酸サンプル調製の方法 |
| EP4353830A3 (en) | 2016-09-15 | 2024-07-03 | ArcherDX, LLC | Methods of nucleic acid sample preparation for analysis of cell-free dna |
| KR102522023B1 (ko) | 2016-09-26 | 2023-04-17 | 셀룰러 리서치, 인크. | 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정 |
| EP3518974A4 (en) | 2016-09-29 | 2020-05-27 | Myriad Women's Health, Inc. | NON-INVASIVE PRENATAL SCREENING USING DYNAMIC ITERATIVE DEEP OPTIMIZATION |
| WO2018067517A1 (en) | 2016-10-04 | 2018-04-12 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| US10011870B2 (en) | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
| CA3207879A1 (en) | 2017-01-24 | 2018-08-02 | Sequenom, Inc. | Methods and processes for assessment of genetic variations |
| EP3577232A1 (en) | 2017-02-01 | 2019-12-11 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
| EP3585889A1 (en) | 2017-02-21 | 2020-01-01 | Natera, Inc. | Compositions, methods, and kits for isolating nucleic acids |
| EP3998350B1 (en) | 2017-03-17 | 2025-05-07 | Sequenom, Inc. | Methods and processes for assessment of genetic mosaicism |
| EP3602359A4 (en) | 2017-03-24 | 2021-01-06 | Myriad Women's Health, Inc. | NUMBER OF COPIES VARIANT CALLER |
| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| CN107012226A (zh) * | 2017-04-20 | 2017-08-04 | 司法部司法鉴定科学技术研究所 | 一种基于高通量测序的snp位点的检测试剂盒及其检测方法 |
| KR102790050B1 (ko) | 2017-06-05 | 2025-04-04 | 백톤 디킨슨 앤드 컴퍼니 | 단일 세포를 위한 샘플 인덱싱 |
| CN107217095B (zh) * | 2017-06-15 | 2021-06-04 | 广东腾飞基因科技股份有限公司 | 人类亲权鉴定用多重pcr引物组及检测方法 |
| US11646100B2 (en) | 2017-08-04 | 2023-05-09 | Billiontoone, Inc. | Target-associated molecules for characterization associated with biological targets |
| BR112020002555A2 (pt) | 2017-08-07 | 2020-08-11 | The Johns Hopkins University | métodos e materiais para avaliar e tratar câncer |
| CN107557458A (zh) * | 2017-10-11 | 2018-01-09 | 华东医药（杭州）基因科技有限公司 | 一种有效检测基因型的方法及装置 |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| AU2018355575A1 (en) | 2017-10-27 | 2020-05-21 | Juno Diagnostics, Inc. | Devices, systems and methods for ultra-low volume liquid biopsy |
| CN108048575B (zh) * | 2017-11-03 | 2021-06-15 | 中南大学湘雅医院 | 一种用于产前无创亲子鉴定的试剂盒及方法 |
| WO2019118926A1 (en) | 2017-12-14 | 2019-06-20 | Tai Diagnostics, Inc. | Assessing graft suitability for transplantation |
| WO2019161244A1 (en) | 2018-02-15 | 2019-08-22 | Natera, Inc. | Methods for isolating nucleic acids with size selection |
| CA3095030A1 (en) | 2018-03-30 | 2019-10-03 | Juno Diagnostics, Inc. | Deep learning-based methods, devices, and systems for prenatal testing |
| CA3090426A1 (en) | 2018-04-14 | 2019-10-17 | Natera, Inc. | Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna |
| EP4545647A3 (en) | 2018-05-03 | 2025-07-09 | Becton, Dickinson and Company | Molecular barcoding on opposite transcript ends |
| WO2019213294A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
| CN108875307B (zh) * | 2018-06-29 | 2021-12-03 | 上海欧易生物医学科技有限公司 | 一种基于孕妇外周血中胎儿游离dna的亲子鉴定方法 |
| US12234509B2 (en) | 2018-07-03 | 2025-02-25 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
| EP3847653A2 (en) * | 2018-09-07 | 2021-07-14 | Illumina, Inc. | A method to determine if a circulating fetal cell isolated from a pregnant mother is from either the current or a historical pregnancy |
| EP4471156A3 (en) | 2018-10-01 | 2025-02-26 | Becton, Dickinson and Company | Determining 5' transcript sequences |
| JP7618548B2 (ja) | 2018-11-08 | 2025-01-21 | ベクトン・ディキンソン・アンド・カンパニー | ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析 |
| WO2020123384A1 (en) | 2018-12-13 | 2020-06-18 | Cellular Research, Inc. | Selective extension in single cell whole transcriptome analysis |
| CN110964690A (zh) * | 2019-01-16 | 2020-04-07 | 北京大学深圳医院 | 辅助生殖技术实施过程中防止配子错配的方法 |
| ES2989967T3 (es) | 2019-01-23 | 2024-11-28 | Becton Dickinson Co | Oligonucleótidos asociados con anticuerpos |
| US12071617B2 (en) | 2019-02-14 | 2024-08-27 | Becton, Dickinson And Company | Hybrid targeted and whole transcriptome amplification |
| EP3980559A1 (en) | 2019-06-06 | 2022-04-13 | Natera, Inc. | Methods for detecting immune cell dna and monitoring immune system |
| EP3987525A1 (en) * | 2019-06-21 | 2022-04-27 | CooperSurgical, Inc. | System and method for determining genetic relationships between a sperm provider, oocyte provider, and the respective conceptus |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| CN112466397A (zh) * | 2019-09-09 | 2021-03-09 | 深圳乐土生物科技有限公司 | 一种用于亲缘关系检测的方法和装置 |
| CN114729350A (zh) | 2019-11-08 | 2022-07-08 | 贝克顿迪金森公司 | 使用随机引发获得用于免疫组库测序的全长v(d)j信息 |
| CN110724732A (zh) * | 2019-11-12 | 2020-01-24 | 陈洪亮 | 一种产前亲权关系鉴定的方法 |
| WO2021137770A1 (en) * | 2019-12-30 | 2021-07-08 | Geneton S.R.O. | Method for fetal fraction estimation based on detection and interpretation of single nucleotide variants |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| US12059674B2 (en) | 2020-02-03 | 2024-08-13 | Tecan Genomics, Inc. | Reagent storage system |
| CA3170345A1 (en) | 2020-02-14 | 2021-08-19 | The Johns Hopkins University | Methods and materials for assessing nucleic acids |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| WO2021174079A2 (en) * | 2020-02-28 | 2021-09-02 | Laboratory Corporation Of America Holdings | Compositions, methods, and systems for paternity determination |
| CN111518917B (zh) * | 2020-04-02 | 2022-06-07 | 中山大学 | 一种用于无创产前亲权关系判定的微单倍型遗传标记组合及方法 |
| EP4150118A1 (en) | 2020-05-14 | 2023-03-22 | Becton Dickinson and Company | Primers for immune repertoire profiling |
| EP4158055B1 (en) | 2020-06-02 | 2024-03-27 | Becton, Dickinson and Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2022026909A1 (en) | 2020-07-31 | 2022-02-03 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| CN112053743B (zh) * | 2020-08-31 | 2024-11-22 | 济宁医学院 | 一种亲缘关系鉴定方法、系统及设备 |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| IL303256A (en) | 2020-12-02 | 2023-07-01 | Genentech Inc | Methods and compositions for the treatment of neoadjuvant and adjuvant carcinoma of the urinary tract |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| WO2022131328A1 (ja) | 2020-12-16 | 2022-06-23 | 株式会社seeDNA | 多型座位の信号の信頼性値の算出方法 |
| WO2022204322A1 (en) * | 2021-03-23 | 2022-09-29 | Billiontoone, Inc. | Fragment analysis for quantitative diagnostics of biological targets |
| CN113549701B (zh) * | 2021-07-21 | 2022-07-01 | 内蒙古农业大学 | 一种山羊亲子鉴定snp分子标记及其应用 |
| JP2024528932A (ja) | 2021-08-02 | 2024-08-01 | ナテラ， インコーポレイテッド | 妊娠中の女性における新生物を検出するための方法 |
| CN118613594A (zh) | 2021-09-01 | 2024-09-06 | 纳特拉公司 | 用于非侵入性产前测试的方法 |
| CN113981062B (zh) * | 2021-10-14 | 2024-02-20 | 武汉蓝沙医学检验实验室有限公司 | 以非生父和母亲dna评估胎儿dna浓度的方法及应用 |
| CN113969310B (zh) * | 2021-10-14 | 2024-02-20 | 武汉蓝沙医学检验实验室有限公司 | 胎儿dna浓度的评估方法及应用 |
| CN113889189B (zh) * | 2021-10-14 | 2025-05-16 | 武汉蓝沙医学检验实验室有限公司 | 以生父和母亲dna评估胎儿dna浓度的方法及应用 |
| CN113999900B (zh) * | 2021-10-14 | 2024-02-20 | 武汉蓝沙医学检验实验室有限公司 | 以孕妇游离dna评估胎儿dna浓度的方法及应用 |
| JP7331325B1 (ja) | 2022-08-30 | 2023-08-23 | 株式会社seeDNA | ２種以上の検査を実施可能な遺伝学的解析方法 |
| CN116240274A (zh) * | 2022-11-10 | 2023-06-09 | 武汉蓝沙医学检验实验室有限公司 | 无需父本对妊娠胎儿进行亲子鉴定的方法 |
| CN117116359A (zh) * | 2023-08-07 | 2023-11-24 | 天津大学 | Dna存储中基于最大后验概率的鲁棒多序列重建方法 |
| JP7684727B2 (ja) * | 2023-08-22 | 2025-05-28 | 株式会社seeDNA | 非侵襲的出生前親子鑑定方法 |
| CN117487932B (zh) * | 2023-11-22 | 2024-09-03 | 复旦大学 | 一种用于亲子鉴定的snp位点组合、及其检测引物对和应用 |
Family Cites Families (329)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4935342A (en) | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
| SU1552642A3 (ru) * | 1987-12-07 | 1994-03-15 | Институт молекулярной генетики РАН | Способ определения родства живых организмов |
| US5153117A (en) * | 1990-03-27 | 1992-10-06 | Genetype A.G. | Fetal cell recovery method |
| US6582908B2 (en) | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| IL103935A0 (en) | 1991-12-04 | 1993-05-13 | Du Pont | Method for the identification of microorganisms by the utilization of directed and arbitrary dna amplification |
| GB9305984D0 (en) | 1993-03-23 | 1993-05-12 | Royal Free Hosp School Med | Predictive assay |
| WO1995006137A1 (en) | 1993-08-27 | 1995-03-02 | Australian Red Cross Society | Detection of genes |
| SE9400522D0 (sv) | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| US5716776A (en) | 1994-03-04 | 1998-02-10 | Mark H. Bogart | Enrichment by preferential mitosis of fetal lymphocytes from a maternal blood sample |
| US6025128A (en) | 1994-09-29 | 2000-02-15 | The University Of Tulsa | Prediction of prostate cancer progression by analysis of selected predictive parameters |
| US6479235B1 (en) | 1994-09-30 | 2002-11-12 | Promega Corporation | Multiplex amplification of short tandem repeat loci |
| US6720140B1 (en) | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
| US5733729A (en) | 1995-09-14 | 1998-03-31 | Affymetrix, Inc. | Computer-aided probability base calling for arrays of nucleic acid probes on chips |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US6852487B1 (en) | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| WO1997034015A1 (en) | 1996-03-15 | 1997-09-18 | The Penn State Research Foundation | Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays |
| US6108635A (en) | 1996-05-22 | 2000-08-22 | Interleukin Genetics, Inc. | Integrated disease information system |
| US6100029A (en) | 1996-08-14 | 2000-08-08 | Exact Laboratories, Inc. | Methods for the detection of chromosomal aberrations |
| US6300077B1 (en) | 1996-08-14 | 2001-10-09 | Exact Sciences Corporation | Methods for the detection of nucleic acids |
| US6221654B1 (en) | 1996-09-25 | 2001-04-24 | California Institute Of Technology | Method and apparatus for analysis and sorting of polynucleotides based on size |
| US5860917A (en) | 1997-01-15 | 1999-01-19 | Chiron Corporation | Method and apparatus for predicting therapeutic outcomes |
| US5824467A (en) | 1997-02-25 | 1998-10-20 | Celtrix Pharmaceuticals | Methods for predicting drug response |
| US20010051341A1 (en) | 1997-03-04 | 2001-12-13 | Isis Innovation Limited | Non-invasive prenatal diagnosis |
| GB9704444D0 (en) | 1997-03-04 | 1997-04-23 | Isis Innovation | Non-invasive prenatal diagnosis |
| EP0866071B1 (en) | 1997-03-20 | 2004-10-20 | F. Hoffmann-La Roche Ag | Modified primers |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| US5994148A (en) | 1997-06-23 | 1999-11-30 | The Regents Of University Of California | Method of predicting and enhancing success of IVF/ET pregnancy |
| US6833242B2 (en) | 1997-09-23 | 2004-12-21 | California Institute Of Technology | Methods for detecting and sorting polynucleotides based on size |
| US6124120A (en) | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
| WO2000066605A2 (en) | 1999-04-30 | 2000-11-09 | Cyclops Genome Sciences Limited | Polynucleotides |
| US6180349B1 (en) | 1999-05-18 | 2001-01-30 | The Regents Of The University Of California | Quantitative PCR method to enumerate DNA copy number |
| US7058517B1 (en) | 1999-06-25 | 2006-06-06 | Genaissance Pharmaceuticals, Inc. | Methods for obtaining and using haplotype data |
| US6964847B1 (en) | 1999-07-14 | 2005-11-15 | Packard Biosciences Company | Derivative nucleic acids and uses thereof |
| GB9917307D0 (en) | 1999-07-23 | 1999-09-22 | Sec Dep Of The Home Department | Improvements in and relating to analysis of DNA |
| US6440706B1 (en) | 1999-08-02 | 2002-08-27 | Johns Hopkins University | Digital amplification |
| US6251604B1 (en) | 1999-08-13 | 2001-06-26 | Genopsys, Inc. | Random mutagenesis and amplification of nucleic acid |
| US6221603B1 (en) | 2000-02-04 | 2001-04-24 | Molecular Dynamics, Inc. | Rolling circle amplification assay for nucleic acid analysis |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US7510834B2 (en) | 2000-04-13 | 2009-03-31 | Hidetoshi Inoko | Gene mapping method using microsatellite genetic polymorphism markers |
| GB0009179D0 (en) | 2000-04-13 | 2000-05-31 | Imp College Innovations Ltd | Non-invasive prenatal diagnosis |
| AU2001274869A1 (en) | 2000-05-20 | 2001-12-03 | The Regents Of The University Of Michigan | Method of producing a dna library using positional amplification |
| AU2001264811A1 (en) | 2000-05-23 | 2001-12-03 | Vincent P. Stanton Jr. | Methods for genetic analysis of dna to detect sequence variances |
| US20030009295A1 (en) | 2001-03-14 | 2003-01-09 | Victor Markowitz | System and method for retrieving and using gene expression data from multiple sources |
| CA2410879A1 (en) | 2000-06-07 | 2001-12-13 | Baylor College Of Medicine | Novel compositions and methods for array-based nucleic acid hybridization |
| US7058616B1 (en) | 2000-06-08 | 2006-06-06 | Virco Bvba | Method and system for predicting resistance of a disease to a therapeutic agent using a neural network |
| GB0016742D0 (en) | 2000-07-10 | 2000-08-30 | Simeg Limited | Diagnostic method |
| US20020107640A1 (en) | 2000-11-14 | 2002-08-08 | Ideker Trey E. | Methods for determining the true signal of an analyte |
| CA2428757A1 (en) | 2000-11-15 | 2002-07-18 | Roche Diagnostics Corporation | Methods and reagents for identifying rare fetal cells in the maternal circulation |
| US7218764B2 (en) | 2000-12-04 | 2007-05-15 | Cytokinetics, Inc. | Ploidy classification method |
| JP4276843B2 (ja) | 2001-03-23 | 2009-06-10 | 芳之 金井 | モノヌクレオソーム及びその製造方法、ヌクレオソームに特異的な抗体の測定方法、自己免疫病診断方法、ヌクレオソームｄｎａ製造方法、ｄｎａプレート、ｄｎａプレート製造方法、並びに抗ｄｎａ抗体測定法 |
| US6489135B1 (en) | 2001-04-17 | 2002-12-03 | Atairgintechnologies, Inc. | Determination of biological characteristics of embryos fertilized in vitro by assaying for bioactive lipids in culture media |
| FR2824144B1 (fr) | 2001-04-30 | 2004-09-17 | Metagenex S A R L | Methode de diagnostic prenatal sur cellule foetale isolee du sang maternel |
| US7392199B2 (en) | 2001-05-01 | 2008-06-24 | Quest Diagnostics Investments Incorporated | Diagnosing inapparent diseases from common clinical tests using Bayesian analysis |
| US7262030B2 (en) | 2001-05-09 | 2007-08-28 | Virginia Commonwealth University | Multiple sequencible and ligatible structures for genomic analysis |
| US20030101000A1 (en) | 2001-07-24 | 2003-05-29 | Bader Joel S. | Family based tests of association using pooled DNA and SNP markers |
| US6958211B2 (en) | 2001-08-08 | 2005-10-25 | Tibotech Bvba | Methods of assessing HIV integrase inhibitor therapy |
| US6807491B2 (en) | 2001-08-30 | 2004-10-19 | Hewlett-Packard Development Company, L.P. | Method and apparatus for combining gene predictions using bayesian networks |
| AUPR749901A0 (en) | 2001-09-06 | 2001-09-27 | Monash University | Method of identifying chromosomal abnormalities and prenatal diagnosis |
| US8986944B2 (en) | 2001-10-11 | 2015-03-24 | Aviva Biosciences Corporation | Methods and compositions for separating rare cells from fluid samples |
| EP1442139A4 (en) | 2001-10-12 | 2005-01-26 | Univ Queensland | SELECTION AND AMPLIFICATION OF MULTIPLE GENETIC MARKERS |
| US7297485B2 (en) | 2001-10-15 | 2007-11-20 | Qiagen Gmbh | Method for nucleic acid amplification that results in low amplification bias |
| US20030119004A1 (en) | 2001-12-05 | 2003-06-26 | Wenz H. Michael | Methods for quantitating nucleic acids using coupled ligation and amplification |
| US20050214758A1 (en) | 2001-12-11 | 2005-09-29 | Netech Inc. | Blood cell separating system |
| JP2005514956A (ja) | 2002-01-18 | 2005-05-26 | ジェンザイム・コーポレーション | 胎児ｄｎａの検出および対立遺伝子の定量化のための方法 |
| JP2005516310A (ja) | 2002-02-01 | 2005-06-02 | ロゼッタ インファーマティクス エルエルシー | 遺伝子を特定し、形質に関連する経路を明らかにするコンピュータ・システムおよび方法 |
| US6977162B2 (en) | 2002-03-01 | 2005-12-20 | Ravgen, Inc. | Rapid analysis of variations in a genome |
| IL163600A0 (en) | 2002-03-01 | 2005-12-18 | Ravgen Inc | Methods for detection of genetic disorders |
| US20060229823A1 (en) | 2002-03-28 | 2006-10-12 | Affymetrix, Inc. | Methods and computer software products for analyzing genotyping data |
| WO2003093426A2 (en) | 2002-05-02 | 2003-11-13 | University Of North Carolina At Chapel Hill | In vitro mutagenesis, phenotyping, and gene mapping |
| US7727720B2 (en) | 2002-05-08 | 2010-06-01 | Ravgen, Inc. | Methods for detection of genetic disorders |
| US7442506B2 (en) | 2002-05-08 | 2008-10-28 | Ravgen, Inc. | Methods for detection of genetic disorders |
| US20070178478A1 (en) | 2002-05-08 | 2007-08-02 | Dhallan Ravinder S | Methods for detection of genetic disorders |
| US20040229231A1 (en) | 2002-05-28 | 2004-11-18 | Frudakis Tony N. | Compositions and methods for inferring ancestry |
| CA2492631C (en) | 2002-05-31 | 2013-03-12 | Genetic Technologies Limited | Maternal antibodies as fetal cell markers to identify and enrich fetal cells from maternal blood |
| WO2003106623A2 (en) | 2002-06-13 | 2003-12-24 | New York University | Early noninvasive prenatal test for aneuploidies and heritable conditions |
| US20050009069A1 (en) | 2002-06-25 | 2005-01-13 | Affymetrix, Inc. | Computer software products for analyzing genotyping |
| EP1388812A1 (en) | 2002-07-04 | 2004-02-11 | Ronald E. Dr. Kates | Method for training a learning-capable system |
| US20040117346A1 (en) | 2002-09-20 | 2004-06-17 | Kilian Stoffel | Computer-based method and apparatus for repurposing an ontology |
| US7459273B2 (en) | 2002-10-04 | 2008-12-02 | Affymetrix, Inc. | Methods for genotyping selected polymorphism |
| WO2004033649A2 (en) | 2002-10-07 | 2004-04-22 | University Of Medicine And Dentistry Of New Jersey | High throughput multiplex dna sequence amplifications |
| AU2003291481A1 (en) | 2002-11-11 | 2004-06-03 | Affymetrix, Inc. | Methods for identifying dna copy number changes |
| US20040224331A1 (en) | 2003-01-17 | 2004-11-11 | The Trustees Of Boston University | Haplotype analysis |
| WO2004065628A1 (en) | 2003-01-21 | 2004-08-05 | Guoliang Fu | Quantitative multiplex detection of nucleic acids |
| EP1594980B2 (en) | 2003-01-29 | 2017-08-16 | 454 Life Sciences Corporation | Bead emulsion nucleic acid amplification |
| US20040209299A1 (en) | 2003-03-07 | 2004-10-21 | Rubicon Genomics, Inc. | In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA |
| US20040197832A1 (en) | 2003-04-03 | 2004-10-07 | Mor Research Applications Ltd. | Non-invasive prenatal genetic diagnosis using transcervical cells |
| JP2004348437A (ja) | 2003-05-22 | 2004-12-09 | Matsushita Electric Ind Co Ltd | リソース管理装置、リソース管理方法及び記録媒体 |
| WO2005000006A2 (en) | 2003-05-28 | 2005-01-06 | Pioneer Hi-Bred International, Inc. | Plant breeding method |
| US20040259100A1 (en) | 2003-06-20 | 2004-12-23 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| WO2005012578A1 (en) | 2003-07-31 | 2005-02-10 | Sequenom, Inc. | Methods for high level multiplexed polymerase chain reactions and homogeneous mass extension reactions for genotyping of polymorphisms |
| WO2005021793A1 (en) | 2003-08-29 | 2005-03-10 | Pantarhei Bioscience B.V. | Prenatal diagnosis of down syndrome by detection of fetal rna markers in maternal blood |
| WO2005023091A2 (en) | 2003-09-05 | 2005-03-17 | The Trustees Of Boston University | Method for non-invasive prenatal diagnosis |
| US20050053950A1 (en) | 2003-09-08 | 2005-03-10 | Enrique Zudaire Ubani | Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons |
| EP1669447A4 (en) | 2003-09-24 | 2007-03-14 | Kyushu Tlo Co Ltd | SNPS IN THE 5 'REGULATORY AREA OF THE MDR1 GENE |
| EP2395111B1 (en) | 2003-10-08 | 2015-05-13 | Trustees of Boston University | Methods for prenatal diagnosis of chromosomal abnormalities |
| CA2482097C (en) | 2003-10-13 | 2012-02-21 | F. Hoffmann-La Roche Ag | Methods for isolating nucleic acids |
| ATE435301T1 (de) | 2003-10-16 | 2009-07-15 | Sequenom Inc | Nicht invasiver nachweis fötaler genetischer merkmale |
| WO2005039389A2 (en) | 2003-10-22 | 2005-05-06 | 454 Corporation | Sequence-based karyotyping |
| EP1678329A4 (en) | 2003-10-30 | 2008-07-02 | Tufts New England Medical Ct | PRENATAL DIAGNOSIS USING CELL-FREE FEDERAL DNA IN FRUIT WATER |
| WO2005071078A1 (en) | 2004-01-12 | 2005-08-04 | Nimblegen Systems Inc. | Method of performing pcr amplification on a microarray |
| US20100216153A1 (en) | 2004-02-27 | 2010-08-26 | Helicos Biosciences Corporation | Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities |
| US7035740B2 (en) | 2004-03-24 | 2006-04-25 | Illumina, Inc. | Artificial intelligence and global normalization methods for genotyping |
| JP4437050B2 (ja) | 2004-03-26 | 2010-03-24 | 株式会社日立製作所 | 診断支援システム、診断支援方法および診断支援サービスの提供方法 |
| WO2005094363A2 (en) | 2004-03-30 | 2005-10-13 | New York University | System, method and software arrangement for bi-allele haplotype phasing |
| DE602005018028D1 (de) | 2004-03-31 | 2010-01-14 | Adnagen Ag | Monoklonale antikörper mit spezifität für fetale erythroidzellen |
| US7414118B1 (en) | 2004-04-14 | 2008-08-19 | Applied Biosystems Inc. | Modified oligonucleotides and applications thereof |
| US7468249B2 (en) | 2004-05-05 | 2008-12-23 | Biocept, Inc. | Detection of chromosomal disorders |
| US7709194B2 (en) | 2004-06-04 | 2010-05-04 | The Chinese University Of Hong Kong | Marker for prenatal diagnosis and monitoring |
| KR20070034588A (ko) | 2004-06-14 | 2007-03-28 | 더 보오드 오브 트러스티스 오브 더 유니버시티 오브 일리노이즈 | Ｃｄ３４＋／ｃｄ３６＋ 태아 세포에는 결합하지만 성체세포에는 결합하지 않는 항체 |
| EP1786924A4 (en) | 2004-07-06 | 2008-10-01 | Genera Biosystems Pty Ltd | METHOD FOR DETECTING ANEUPLOIDIA |
| DE102004036285A1 (de) | 2004-07-27 | 2006-02-16 | Advalytix Ag | Verfahren zum Bestimmen der Häufigkeit von Sequenzen einer Probe |
| US20060040300A1 (en) | 2004-08-09 | 2006-02-23 | Generation Biotech, Llc | Method for nucleic acid isolation and amplification |
| JP2008511058A (ja) | 2004-08-18 | 2008-04-10 | アボツト・モレキユラー・インコーポレイテツド | コンピュータシステムを用いるデータ品質および／または部分異数染色体の決定 |
| US8024128B2 (en) | 2004-09-07 | 2011-09-20 | Gene Security Network, Inc. | System and method for improving clinical decisions by aggregating, validating and analysing genetic and phenotypic data |
| US20060088574A1 (en) | 2004-10-25 | 2006-04-27 | Manning Paul B | Nutritional supplements |
| US20060134662A1 (en) | 2004-10-25 | 2006-06-22 | Pratt Mark R | Method and system for genotyping samples in a normalized allelic space |
| EP1828419A1 (en) | 2004-11-17 | 2007-09-05 | ReproCure, LLC | Methods of determining human egg competency |
| US20070042384A1 (en) | 2004-12-01 | 2007-02-22 | Weiwei Li | Method for isolating and modifying DNA from blood and body fluids |
| WO2006091979A2 (en) | 2005-02-25 | 2006-08-31 | The Regents Of The University Of California | Full karyotype single cell chromosome analysis |
| US7618777B2 (en) | 2005-03-16 | 2009-11-17 | Agilent Technologies, Inc. | Composition and method for array hybridization |
| EP2161347B1 (en) | 2005-03-18 | 2016-08-24 | The Chinese University Of Hong Kong | Markers for prenatal diagnosis and monitoring |
| AU2006224971B2 (en) | 2005-03-18 | 2009-07-02 | Boston University | A method for the detection of chromosomal aneuploidies |
| EP1896617B1 (en) | 2005-05-31 | 2013-01-02 | Life Technologies Corporation | Multiplex amplification of short nucleic acids |
| EP1907571B1 (en) | 2005-06-15 | 2017-04-26 | Complete Genomics Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
| US20070020640A1 (en) | 2005-07-21 | 2007-01-25 | Mccloskey Megan L | Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis |
| RU2290078C1 (ru) | 2005-07-25 | 2006-12-27 | Евгений Владимирович Новичков | Способ прогнозирования рецидива серозного рака яичников |
| US10083273B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US20070027636A1 (en) | 2005-07-29 | 2007-02-01 | Matthew Rabinowitz | System and method for using genetic, phentoypic and clinical data to make predictions for clinical or lifestyle decisions |
| US10081839B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US8532930B2 (en) | 2005-11-26 | 2013-09-10 | Natera, Inc. | Method for determining the number of copies of a chromosome in the genome of a target individual using genetic data from genetically related individuals |
| US8515679B2 (en) | 2005-12-06 | 2013-08-20 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
| US20070178501A1 (en) | 2005-12-06 | 2007-08-02 | Matthew Rabinowitz | System and method for integrating and validating genotypic, phenotypic and medical information into a database according to a standardized ontology |
| WO2007018601A1 (en) | 2005-08-02 | 2007-02-15 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction |
| GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| US20090228299A1 (en) | 2005-11-09 | 2009-09-10 | The Regents Of The University Of California | Methods and apparatus for context-sensitive telemedicine |
| GB0523276D0 (en) | 2005-11-15 | 2005-12-21 | London Bridge Fertility | Chromosomal analysis by molecular karyotyping |
| AU2006318425B2 (en) | 2005-11-26 | 2013-05-02 | Natera, Inc. | System and method for cleaning noisy genetic data and using data to make predictions |
| WO2007070482A2 (en) | 2005-12-14 | 2007-06-21 | Xueliang Xia | Microarray-based preimplantation genetic diagnosis of chromosomal abnormalities |
| EP2423334A3 (en) | 2006-02-02 | 2012-04-18 | The Board of Trustees of The Leland Stanford Junior University | Non-invasive fetal genetic screening by digital analysis |
| WO2007092538A2 (en) | 2006-02-07 | 2007-08-16 | President And Fellows Of Harvard College | Methods for making nucleotide probes for sequencing and synthesis |
| EP1991675B1 (en) | 2006-02-08 | 2011-06-29 | Illumina Cambridge Limited | End modification to prevent over-representation of fragments |
| AU2007220991C1 (en) | 2006-02-28 | 2013-08-15 | University Of Louisville Research Foundation | Detecting fetal chromosomal abnormalities using tandem single nucleotide polymorphisms |
| JP2009529880A (ja) | 2006-03-13 | 2009-08-27 | ベリデックス・エルエルシー | 原発細胞の増殖 |
| WO2007112418A2 (en) | 2006-03-28 | 2007-10-04 | Baylor College Of Medicine | Screening for down syndrome |
| US20070243549A1 (en) | 2006-04-12 | 2007-10-18 | Biocept, Inc. | Enrichment of circulating fetal dna |
| US7702468B2 (en) | 2006-05-03 | 2010-04-20 | Population Diagnostics, Inc. | Evaluating genetic disorders |
| US7901884B2 (en) | 2006-05-03 | 2011-03-08 | The Chinese University Of Hong Kong | Markers for prenatal diagnosis and monitoring |
| AU2007260676A1 (en) | 2006-06-14 | 2007-12-21 | Artemis Health, Inc. | Rare cell analysis using sample splitting and DNA tags |
| US20080124721A1 (en) | 2006-06-14 | 2008-05-29 | Martin Fuchs | Analysis of rare cell-enriched samples |
| WO2007147076A2 (en) | 2006-06-14 | 2007-12-21 | Living Microsystems, Inc. | Methods for the diagnosis of fetal abnormalities |
| US8137912B2 (en) | 2006-06-14 | 2012-03-20 | The General Hospital Corporation | Methods for the diagnosis of fetal abnormalities |
| US20080026390A1 (en) | 2006-06-14 | 2008-01-31 | Roland Stoughton | Diagnosis of Fetal Abnormalities by Comparative Genomic Hybridization Analysis |
| EP2589668A1 (en) | 2006-06-14 | 2013-05-08 | Verinata Health, Inc | Rare cell analysis using sample splitting and DNA tags |
| US20080070792A1 (en) | 2006-06-14 | 2008-03-20 | Roland Stoughton | Use of highly parallel snp genotyping for fetal diagnosis |
| US20080026451A1 (en) | 2006-06-15 | 2008-01-31 | Braman Jeffrey C | System for isolating biomolecules from a sample |
| US20080096766A1 (en) | 2006-06-16 | 2008-04-24 | Sequenom, Inc. | Methods and compositions for the amplification, detection and quantification of nucleic acid from a sample |
| US20080182244A1 (en) | 2006-08-04 | 2008-07-31 | Ikonisys, Inc. | Pre-Implantation Genetic Diagnosis Test |
| US9273309B2 (en) | 2006-08-24 | 2016-03-01 | University Of Massachusetts | Mapping of genomic interactions |
| WO2008143627A2 (en) | 2006-09-14 | 2008-11-27 | Ibis Biosciences, Inc. | Targeted whole genome amplification method for identification of pathogens |
| US20080085836A1 (en) | 2006-09-22 | 2008-04-10 | Kearns William G | Method for genetic testing of human embryos for chromosome abnormalities, segregating genetic disorders with or without a known mutation and mitochondrial disorders following in vitro fertilization (IVF), embryo culture and embryo biopsy |
| AU2007311126A1 (en) | 2006-10-16 | 2008-04-24 | Celula Inc. | Methods and compositions for differential expansion of fetal cells in maternal blood and their use |
| US20100184152A1 (en) | 2006-10-23 | 2010-07-22 | Vladislav Sandler | Target-oriented whole genome amplification of nucleic acids |
| WO2008059578A1 (en) | 2006-11-16 | 2008-05-22 | Olympus Corporation | Multiplex pcr method |
| WO2008081451A2 (en) * | 2007-01-03 | 2008-07-10 | Monaliza Medical Ltd. | Methods and kits for analyzing genetic material of a fetus |
| WO2008098142A2 (en) | 2007-02-08 | 2008-08-14 | Sequenom, Inc. | Nucleic acid-based tests for rhd typing, gender determination and nucleic acid quantification |
| CN101790731B (zh) | 2007-03-16 | 2013-11-06 | 纳特拉公司 | 用于清除遗传数据干扰并确定染色体拷贝数的系统和方法 |
| ITTO20070307A1 (it) | 2007-05-04 | 2008-11-05 | Silicon Biosystems Spa | Metodo e dispositivo per la diagnosi prenatale non-invasiva |
| CN101849185A (zh) | 2007-05-31 | 2010-09-29 | 加利福尼亚大学董事会 | 基于空间位阻和酶相关信号放大的高特异性和高灵敏度检测 |
| WO2008157264A2 (en) | 2007-06-15 | 2008-12-24 | Sequenom, Inc. | Combined methods for the detection of chromosomal aneuploidy |
| US20090023190A1 (en) | 2007-06-20 | 2009-01-22 | Kai Qin Lao | Sequence amplification with loopable primers |
| EP2162551B1 (en) | 2007-07-03 | 2017-08-23 | Genaphora LTD. | Chimeric primers for improved nucleic acid amplification reactions |
| WO2009009769A2 (en) | 2007-07-11 | 2009-01-15 | Artemis Health, Inc. | Diagnosis of fetal abnormalities using nucleated red blood cells |
| CN106886688B (zh) | 2007-07-23 | 2020-07-10 | 香港中文大学 | 用于分析癌症相关的遗传变异的系统 |
| US12180549B2 (en) | 2007-07-23 | 2024-12-31 | The Chinese University Of Hong Kong | Diagnosing fetal chromosomal aneuploidy using genomic sequencing |
| US20090053719A1 (en) | 2007-08-03 | 2009-02-26 | The Chinese University Of Hong Kong | Analysis of nucleic acids by digital pcr |
| US8568994B2 (en) | 2007-08-03 | 2013-10-29 | Dkfz Deutsches Krebsforschungszentrum, Stiftung Des Offentlichen Rechts | Method for prenatal diagnosis |
| EP2195452B1 (en) | 2007-08-29 | 2012-03-14 | Sequenom, Inc. | Methods and compositions for universal size-specific polymerase chain reaction |
| WO2009032779A2 (en) | 2007-08-29 | 2009-03-12 | Sequenom, Inc. | Methods and compositions for the size-specific seperation of nucleic acid from a sample |
| US8748100B2 (en) | 2007-08-30 | 2014-06-10 | The Chinese University Of Hong Kong | Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences |
| EA018555B1 (ru) | 2007-09-07 | 2013-08-30 | Флуидигм Корпорейшн | Определение вариаций количества копий, способы и системы |
| CA2697640C (en) | 2007-09-21 | 2016-06-21 | Katholieke Universiteit Leuven | Tools and methods for genetic tests using next generation sequencing |
| WO2009042457A1 (en) | 2007-09-21 | 2009-04-02 | Streck, Inc. | Nucleic acid isolation in preserved whole blood |
| WO2009070558A1 (en) | 2007-11-30 | 2009-06-04 | Ge Healthcare Bio-Sciences Corp. | Method for isolation of genomic dna, rna and proteins from a single sample |
| EP2077337A1 (en) | 2007-12-26 | 2009-07-08 | Eppendorf Array Technologies SA | Amplification and detection composition, method and kit |
| US8852864B2 (en) | 2008-01-17 | 2014-10-07 | Sequenom Inc. | Methods and compositions for the analysis of nucleic acids |
| US9034580B2 (en) | 2008-01-17 | 2015-05-19 | Sequenom, Inc. | Single molecule nucleic acid sequence analysis processes and compositions |
| US20110033862A1 (en) | 2008-02-19 | 2011-02-10 | Gene Security Network, Inc. | Methods for cell genotyping |
| US20090221620A1 (en) * | 2008-02-20 | 2009-09-03 | Celera Corporation | Gentic polymorphisms associated with stroke, methods of detection and uses thereof |
| EP2271772B1 (en) | 2008-03-11 | 2014-07-16 | Sequenom, Inc. | Nucleic acid-based tests for prenatal gender determination |
| CN102066573A (zh) | 2008-04-03 | 2011-05-18 | 哈森阿尔法生物技术研究院 | 用于扩增多个靶标的扩增子拯救多重聚合酶链式反应 |
| WO2009146335A1 (en) | 2008-05-27 | 2009-12-03 | Gene Security Network, Inc. | Methods for embryo characterization and comparison |
| EP2128169A1 (de) | 2008-05-30 | 2009-12-02 | Qiagen GmbH | Verfahren zur Isolierung von kurzkettigen Nukleinsäuren |
| CA3116156C (en) | 2008-08-04 | 2023-08-08 | Natera, Inc. | Methods for allele calling and ploidy calling |
| US8697363B2 (en) | 2008-08-26 | 2014-04-15 | Fluidigm Corporation | Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags |
| DE102008045705A1 (de) | 2008-09-04 | 2010-04-22 | Macherey, Nagel Gmbh & Co. Kg Handelsgesellschaft | Verfahren zur Gewinnung von kurzer RNA sowie Kit hierfür |
| US8586310B2 (en) | 2008-09-05 | 2013-11-19 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| JP2012502632A (ja) | 2008-09-17 | 2012-02-02 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | スモールｒｎａの単離法 |
| ES2620012T3 (es) | 2008-09-20 | 2017-06-27 | The Board Of Trustees Of The Leland Stanford Junior University | Diagnóstico no invasivo de la aneuploidia fetal por secuenciación |
| JP2012513217A (ja) | 2008-12-22 | 2012-06-14 | セルラ・インコーポレイテッド | 対立遺伝子、ゲノムおよびトランスクリプトームを検出する方法および遺伝子型決定パネル |
| US11634747B2 (en) | 2009-01-21 | 2023-04-25 | Streck Llc | Preservation of fetal nucleic acids in maternal plasma |
| WO2010088288A2 (en) | 2009-01-28 | 2010-08-05 | Fluidigm Corporation | Determination of copy number differences by amplification |
| DE202010018569U1 (de) | 2009-02-18 | 2017-09-26 | Streck Inc. | Konservierung zellfreier Nukleinsäuren |
| WO2010115044A2 (en) | 2009-04-02 | 2010-10-07 | Fluidigm Corporation | Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation |
| WO2010127186A1 (en) | 2009-04-30 | 2010-11-04 | Prognosys Biosciences, Inc. | Nucleic acid constructs and methods of use |
| KR20110006112A (ko) | 2009-07-13 | 2011-01-20 | 삼성전자주식회사 | 카메라 시스템에서 디스플레이 패널의 백라이트를 제어하는 장치 및 그 방법 |
| US8563242B2 (en) | 2009-08-11 | 2013-10-22 | The Chinese University Of Hong Kong | Method for detecting chromosomal aneuploidy |
| WO2011037692A1 (en) | 2009-09-24 | 2011-03-31 | Qiagen Gaithersburg Inc. | Compositions, methods, and kits for isolating and analyzing nucleic acids using an anion exchange material |
| WO2011041485A1 (en) | 2009-09-30 | 2011-04-07 | Gene Security Network, Inc. | Methods for non-invasive prenatal ploidy calling |
| WO2011057094A1 (en) | 2009-11-05 | 2011-05-12 | The Chinese University Of Hong Kong | Fetal genomic analysis from a maternal biological sample |
| GB2488289A (en) | 2009-11-06 | 2012-08-22 | Univ Leland Stanford Junior | Non-invasive diagnosis of graft rejection in organ transplant patients |
| JP2013511991A (ja) | 2009-11-25 | 2013-04-11 | クアンタライフ， インコーポレイテッド | 遺伝子材料を検出する方法および組成物 |
| CN102802412A (zh) | 2009-12-08 | 2012-11-28 | 海玛奎斯特医药公司 | 用于治疗红细胞病症的方法及低剂量方案 |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9315857B2 (en) | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| EP3088532B1 (en) | 2009-12-22 | 2019-10-30 | Sequenom, Inc. | Processes and kits for identifying aneuploidy |
| US8574842B2 (en) | 2009-12-22 | 2013-11-05 | The Board Of Trustees Of The Leland Stanford Junior University | Direct molecular diagnosis of fetal aneuploidy |
| WO2011090556A1 (en) | 2010-01-19 | 2011-07-28 | Verinata Health, Inc. | Methods for determining fraction of fetal nucleic acid in maternal samples |
| EP3006573B1 (en) | 2010-01-19 | 2018-03-07 | Verinata Health, Inc | Methods for determining fraction of fetal nucleic acids in maternal samples |
| US9323888B2 (en) | 2010-01-19 | 2016-04-26 | Verinata Health, Inc. | Detecting and classifying copy number variation |
| US10388403B2 (en) | 2010-01-19 | 2019-08-20 | Verinata Health, Inc. | Analyzing copy number variation in the detection of cancer |
| US20110312503A1 (en) | 2010-01-23 | 2011-12-22 | Artemis Health, Inc. | Methods of fetal abnormality detection |
| US20130196862A1 (en) | 2010-05-18 | 2013-08-01 | Natera, Inc. | Informatics Enhanced Analysis of Fetal Samples Subject to Maternal Contamination |
| US20190284623A1 (en) | 2010-05-18 | 2019-09-19 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US12152275B2 (en) | 2010-05-18 | 2024-11-26 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| WO2013052557A2 (en) | 2011-10-03 | 2013-04-11 | Natera, Inc. | Methods for preimplantation genetic diagnosis by sequencing |
| US20130123120A1 (en) | 2010-05-18 | 2013-05-16 | Natera, Inc. | Highly Multiplex PCR Methods and Compositions |
| US20190309358A1 (en) | 2010-05-18 | 2019-10-10 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11332785B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11339429B2 (en) | 2010-05-18 | 2022-05-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US20140206552A1 (en) | 2010-05-18 | 2014-07-24 | Natera, Inc. | Methods for preimplantation genetic diagnosis by sequencing |
| US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| EP2854057B1 (en) | 2010-05-18 | 2018-03-07 | Natera, Inc. | Methods for non-invasive pre-natal ploidy calling |
| US20110301854A1 (en) | 2010-06-08 | 2011-12-08 | Curry Bo U | Method of Determining Allele-Specific Copy Number of a SNP |
| US20120034603A1 (en) | 2010-08-06 | 2012-02-09 | Tandem Diagnostics, Inc. | Ligation-based detection of genetic variants |
| US8700338B2 (en) | 2011-01-25 | 2014-04-15 | Ariosa Diagnosis, Inc. | Risk calculation for evaluation of fetal aneuploidy |
| EP2426217A1 (en) | 2010-09-03 | 2012-03-07 | Centre National de la Recherche Scientifique (CNRS) | Analytical methods for cell free nucleic acids and applications |
| EP2633311A4 (en) | 2010-10-26 | 2014-05-07 | Univ Stanford | NONINVASIVE FETAL GENETIC SCREENING THROUGH SEQUENCING ANALYSIS |
| EP4269623A3 (en) | 2010-11-30 | 2024-03-06 | The Chinese University Of Hong Kong | Analysis of a biological sample of an organism for chromosomal deletions or amplifications associated with cancer |
| WO2012078792A2 (en) | 2010-12-07 | 2012-06-14 | Stanford University | Non-invasive determination of fetal inheritance of parental haplotypes at the genome-wide scale |
| US20150064695A1 (en) | 2010-12-17 | 2015-03-05 | Celula Inc. | Methods for screening and diagnosing genetic conditions |
| CA2821906C (en) | 2010-12-22 | 2020-08-25 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| WO2012103031A2 (en) | 2011-01-25 | 2012-08-02 | Ariosa Diagnostics, Inc. | Detection of genetic abnormalities |
| JP6153874B2 (ja) | 2011-02-09 | 2017-06-28 | ナテラ， インコーポレイテッド | 非侵襲的出生前倍数性呼び出しのための方法 |
| EP2902500B1 (en) | 2011-02-09 | 2017-01-11 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| GB2488358A (en) | 2011-02-25 | 2012-08-29 | Univ Plymouth | Enrichment of foetal DNA in maternal plasma |
| WO2012129363A2 (en) | 2011-03-24 | 2012-09-27 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| DK2697392T3 (en) | 2011-04-12 | 2016-03-29 | Verinata Health Inc | SOLUTION OF GENOME FRACTIONS USING Polymorphism COUNTS |
| WO2012142531A2 (en) | 2011-04-14 | 2012-10-18 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| CN110016499B (zh) | 2011-04-15 | 2023-11-14 | 约翰·霍普金斯大学 | 安全测序系统 |
| WO2014014498A1 (en) | 2012-07-20 | 2014-01-23 | Verinata Health, Inc. | Detecting and classifying copy number variation in a fetal genome |
| US9411937B2 (en) | 2011-04-15 | 2016-08-09 | Verinata Health, Inc. | Detecting and classifying copy number variation |
| US9957558B2 (en) | 2011-04-28 | 2018-05-01 | Life Technologies Corporation | Methods and compositions for multiplex PCR |
| EP2546361B1 (en) | 2011-07-11 | 2015-06-03 | Samsung Electronics Co., Ltd. | Method of amplifying target nucleic acid with reduced amplification bias and method for determining relative amount of target nucleic acid in sample |
| US20130024127A1 (en) | 2011-07-19 | 2013-01-24 | John Stuelpnagel | Determination of source contributions using binomial probability calculations |
| GB201115095D0 (en) | 2011-09-01 | 2011-10-19 | Singapore Volition Pte Ltd | Method for detecting nucleosomes containing nucleotides |
| US8712697B2 (en) | 2011-09-07 | 2014-04-29 | Ariosa Diagnostics, Inc. | Determination of copy number variations using binomial probability calculations |
| JP5536729B2 (ja) | 2011-09-20 | 2014-07-02 | 株式会社ソニー・コンピュータエンタテインメント | 情報処理装置、アプリケーション提供システム、アプリケーション提供サーバ、アプリケーション提供方法、および情報処理方法 |
| EP4159857A1 (en) | 2011-09-26 | 2023-04-05 | QIAGEN GmbH | Rapid method for isolating extracellular nucleic acids |
| US9984198B2 (en) | 2011-10-06 | 2018-05-29 | Sequenom, Inc. | Reducing sequence read count error in assessment of complex genetic variations |
| EP2764124B1 (en) | 2011-10-07 | 2017-08-02 | Murdoch Childrens Research Institute | Diagnostic assay for tissue transplantation status |
| WO2013078470A2 (en) | 2011-11-22 | 2013-05-30 | MOTIF, Active | Multiplex isolation of protein-associated nucleic acids |
| US20140364439A1 (en) | 2011-12-07 | 2014-12-11 | The Broad Institute, Inc. | Markers associated with chronic lymphocytic leukemia prognosis and progression |
| US20130190653A1 (en) | 2012-01-25 | 2013-07-25 | Angel Gabriel Alvarez Ramos | Device for blood collection from the placenta and the umbilical cord |
| EP2820155B1 (en) | 2012-02-28 | 2017-07-26 | Population Genetics Technologies Ltd. | Method for attaching a counter sequence to a nucleic acid sample |
| WO2013130848A1 (en) | 2012-02-29 | 2013-09-06 | Natera, Inc. | Informatics enhanced analysis of fetal samples subject to maternal contamination |
| US9862995B2 (en) | 2012-03-13 | 2018-01-09 | Abhijit Ajit Patel | Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing |
| US10385396B2 (en) | 2012-04-19 | 2019-08-20 | The Medical College Of Wisconsin, Inc. | Highly sensitive surveillance using detection of cell free DNA |
| ES2905448T3 (es) | 2012-05-10 | 2022-04-08 | Massachusetts Gen Hospital | Métodos para determinar una secuencia nucleotídica |
| CN104736722B (zh) | 2012-05-21 | 2018-08-07 | 斯克利普斯研究所 | 样品制备方法 |
| WO2013181651A1 (en) | 2012-06-01 | 2013-12-05 | Omega Bio-Tek, Inc. | Selective nucleic acid fragment recovery |
| WO2014004726A1 (en) | 2012-06-26 | 2014-01-03 | Caifu Chen | Methods, compositions and kits for the diagnosis, prognosis and monitoring of cancer |
| KR101890466B1 (ko) | 2012-07-24 | 2018-08-21 | 내테라, 인코포레이티드 | 고도의 다중 ｐｃｒ 방법 및 조성물 |
| US20140051585A1 (en) | 2012-08-15 | 2014-02-20 | Natera, Inc. | Methods and compositions for reducing genetic library contamination |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| CN108103057B (zh) | 2012-08-28 | 2021-09-03 | 阿科尼生物系统公司 | 用于纯化核酸的方法和试剂盒 |
| US20140065621A1 (en) | 2012-09-04 | 2014-03-06 | Natera, Inc. | Methods for increasing fetal fraction in maternal blood |
| ES2769241T5 (es) | 2012-09-04 | 2023-05-30 | Guardant Health Inc | Sistemas y métodos para detectar variación en el número de copias |
| EP2943590A4 (en) | 2013-01-13 | 2017-02-15 | Unitaq Bio | Methods and compositions for pcr using blocked and universal primers |
| WO2014122288A1 (en) | 2013-02-08 | 2014-08-14 | Qiagen Gmbh | Method for separating dna by size |
| US9982255B2 (en) | 2013-03-11 | 2018-05-29 | Kailos Genetics, Inc. | Capture methodologies for circulating cell free DNA |
| US10385394B2 (en) | 2013-03-15 | 2019-08-20 | The Translational Genomics Research Institute | Processes of identifying and characterizing X-linked disorders |
| CN105518151B (zh) | 2013-03-15 | 2021-05-25 | 莱兰斯坦福初级大学评议会 | 循环核酸肿瘤标志物的鉴别和用途 |
| CN114574581A (zh) | 2013-03-15 | 2022-06-03 | 夸登特健康公司 | 检测稀有突变和拷贝数变异的系统和方法 |
| WO2014150300A2 (en) | 2013-03-15 | 2014-09-25 | Abbott Molecular Inc. | Method for amplification and assay of rna fusion gene variants, method of distinguishing same and related primers, probes, and kits |
| US9499870B2 (en) | 2013-09-27 | 2016-11-22 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| US10927408B2 (en) | 2013-12-02 | 2021-02-23 | Personal Genome Diagnostics, Inc. | Method for evaluating minority variants in a sample |
| CN111534580B (zh) | 2013-12-28 | 2024-06-04 | 夸登特健康公司 | 用于检测遗传变异的方法和系统 |
| CN106029962A (zh) | 2014-02-11 | 2016-10-12 | 豪夫迈·罗氏有限公司 | 靶向测序和uid过滤 |
| EP3117012B2 (en) | 2014-03-14 | 2025-11-26 | CareDx, Inc. | Methods of monitoring immunosuppressive therapies in a transplant recipient |
| CA2943636C (en) | 2014-03-25 | 2020-06-09 | Quest Diagnostics Investments Incorporated | Detection of gene fusions by intragenic differential expression (ide) using average cycle thresholds |
| AU2015249846B2 (en) | 2014-04-21 | 2021-07-22 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| WO2015164432A1 (en) | 2014-04-21 | 2015-10-29 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| US20180173845A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| DK3164489T3 (da) | 2014-07-03 | 2020-08-10 | Rhodx Inc | Mærkning og vurdering af en målsekvens |
| WO2016009059A1 (en) | 2014-07-17 | 2016-01-21 | Qiagen Gmbh | Method for isolating rna with high yield |
| US10119167B2 (en) | 2014-07-18 | 2018-11-06 | Illumina, Inc. | Non-invasive prenatal diagnosis of fetal genetic condition using cellular DNA and cell free DNA |
| EP3209403A4 (en) | 2014-10-24 | 2018-09-12 | Abbott Molecular Inc. | Enrichment of small nucleic acids |
| CN107002130B (zh) | 2014-11-11 | 2022-02-01 | 深圳华大基因研究院 | 多程测序 |
| WO2016138080A1 (en) | 2015-02-24 | 2016-09-01 | Trustees Of Boston University | Protection of barcodes during dna amplification using molecular hairpins |
| WO2016138500A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Methods and compositions for barcoding nucleic acids for sequencing |
| EP4180535A1 (en) | 2015-03-30 | 2023-05-17 | Becton, Dickinson and Company | Methods and compositions for combinatorial barcoding |
| KR101850437B1 (ko) | 2015-04-14 | 2018-04-20 | 이원다이애그노믹스(주) | 차세대 염기서열 분석기법을 이용한 장기 이식 거부 반응 예측 방법 |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| US10844428B2 (en) | 2015-04-28 | 2020-11-24 | Illumina, Inc. | Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS) |
| EP3294906B1 (en) | 2015-05-11 | 2024-07-10 | Natera, Inc. | Methods for determining ploidy |
| EP3303630B1 (en) | 2015-06-05 | 2022-01-05 | Qiagen GmbH | Method for separating dna by size |
| EP3356559A4 (en) | 2015-09-29 | 2019-03-06 | Ludwig Institute for Cancer Research Ltd | TYPING AND COMPOSITION OF DISCONTINUOUS GENOMIC ELEMENTS |
| US20190085406A1 (en) | 2016-04-14 | 2019-03-21 | Guardant Health, Inc. | Methods for early detection of cancer |
| WO2017181202A2 (en) | 2016-04-15 | 2017-10-19 | Natera, Inc. | Methods for lung cancer detection |
| US12043856B2 (en) | 2016-05-24 | 2024-07-23 | The Translational Genomics Research Institute | Molecular tagging methods and sequencing libraries |
| WO2017205826A1 (en) | 2016-05-27 | 2017-11-30 | Sequenom, Inc. | Methods for detecting genetic variations |
| CA3025956A1 (en) | 2016-07-01 | 2018-01-04 | Natera, Inc. | Compositions and methods for detection of nucleic acid mutations |
| WO2018067517A1 (en) | 2016-10-04 | 2018-04-12 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| US11608497B2 (en) | 2016-11-08 | 2023-03-21 | Becton, Dickinson And Company | Methods for cell label classification |
| US10011870B2 (en) | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
| EP3585889A1 (en) | 2017-02-21 | 2020-01-01 | Natera, Inc. | Compositions, methods, and kits for isolating nucleic acids |
| US11091800B2 (en) | 2017-09-20 | 2021-08-17 | University Of Utah Research Foundation | Size-selection of cell-free DNA for increasing family size during next-generation sequencing |
| EP3737773B1 (en) | 2018-01-12 | 2023-04-12 | Natera, Inc. | Novel primers and uses thereof |
| WO2019161244A1 (en) | 2018-02-15 | 2019-08-22 | Natera, Inc. | Methods for isolating nucleic acids with size selection |
| CA3090426A1 (en) | 2018-04-14 | 2019-10-17 | Natera, Inc. | Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna |
| WO2019241349A1 (en) | 2018-06-12 | 2019-12-19 | Natera, Inc. | Methods and systems for calling mutations |
| WO2020010255A1 (en) | 2018-07-03 | 2020-01-09 | Natera, Inc. | Methods for detection of donor-derived cell-free dna |
| EP3824470A1 (en) | 2018-07-17 | 2021-05-26 | Natera, Inc. | Methods and systems for calling ploidy states using a neural network |
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