BR112013016193B1 - método ex vivo para determinar se um suposto pai é o pai biológico de um feto que está em gestação em uma gestante e relatório - Google Patents
método ex vivo para determinar se um suposto pai é o pai biológico de um feto que está em gestação em uma gestante e relatório Download PDFInfo
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Families Citing this family (178)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10083273B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US20070178501A1 (en) * | 2005-12-06 | 2007-08-02 | Matthew Rabinowitz | System and method for integrating and validating genotypic, phenotypic and medical information into a database according to a standardized ontology |
| US10081839B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US11111543B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US20070027636A1 (en) * | 2005-07-29 | 2007-02-01 | Matthew Rabinowitz | System and method for using genetic, phentoypic and clinical data to make predictions for clinical or lifestyle decisions |
| US8532930B2 (en) | 2005-11-26 | 2013-09-10 | Natera, Inc. | Method for determining the number of copies of a chromosome in the genome of a target individual using genetic data from genetically related individuals |
| US8515679B2 (en) | 2005-12-06 | 2013-08-20 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
| US11111544B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| WO2009105531A1 (en) * | 2008-02-19 | 2009-08-27 | Gene Security Network, Inc. | Methods for cell genotyping |
| US20110092763A1 (en) * | 2008-05-27 | 2011-04-21 | Gene Security Network, Inc. | Methods for Embryo Characterization and Comparison |
| EP2321642B1 (en) | 2008-08-04 | 2017-01-11 | Natera, Inc. | Methods for allele calling and ploidy calling |
| EP2854056A3 (en) | 2009-09-30 | 2015-06-03 | Natera, Inc. | Methods for non-invasive pre-natal ploidy calling |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US11332793B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11326208B2 (en) | 2010-05-18 | 2022-05-10 | Natera, Inc. | Methods for nested PCR amplification of cell-free DNA |
| US12152275B2 (en) | 2010-05-18 | 2024-11-26 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US12221653B2 (en) | 2010-05-18 | 2025-02-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11332785B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| AU2011255641A1 (en) | 2010-05-18 | 2012-12-06 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11339429B2 (en) | 2010-05-18 | 2022-05-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11203786B2 (en) | 2010-08-06 | 2021-12-21 | Ariosa Diagnostics, Inc. | Detection of target nucleic acids using hybridization |
| US10533223B2 (en) | 2010-08-06 | 2020-01-14 | Ariosa Diagnostics, Inc. | Detection of target nucleic acids using hybridization |
| US20140342940A1 (en) | 2011-01-25 | 2014-11-20 | Ariosa Diagnostics, Inc. | Detection of Target Nucleic Acids using Hybridization |
| US20130261003A1 (en) | 2010-08-06 | 2013-10-03 | Ariosa Diagnostics, In. | Ligation-based detection of genetic variants |
| US20120034603A1 (en) * | 2010-08-06 | 2012-02-09 | Tandem Diagnostics, Inc. | Ligation-based detection of genetic variants |
| US8700338B2 (en) | 2011-01-25 | 2014-04-15 | Ariosa Diagnosis, Inc. | Risk calculation for evaluation of fetal aneuploidy |
| US20130040375A1 (en) | 2011-08-08 | 2013-02-14 | Tandem Diagnotics, Inc. | Assay systems for genetic analysis |
| WO2012088456A2 (en) | 2010-12-22 | 2012-06-28 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| US10131947B2 (en) * | 2011-01-25 | 2018-11-20 | Ariosa Diagnostics, Inc. | Noninvasive detection of fetal aneuploidy in egg donor pregnancies |
| JP6153874B2 (ja) | 2011-02-09 | 2017-06-28 | ナテラ, インコーポレイテッド | 非侵襲的出生前倍数性呼び出しのための方法 |
| CN103748236B (zh) | 2011-04-15 | 2018-12-25 | 约翰·霍普金斯大学 | 安全测序系统 |
| US20140235474A1 (en) | 2011-06-24 | 2014-08-21 | Sequenom, Inc. | Methods and processes for non invasive assessment of a genetic variation |
| US10424394B2 (en) | 2011-10-06 | 2019-09-24 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9984198B2 (en) | 2011-10-06 | 2018-05-29 | Sequenom, Inc. | Reducing sequence read count error in assessment of complex genetic variations |
| US20140242588A1 (en) | 2011-10-06 | 2014-08-28 | Sequenom, Inc | Methods and processes for non-invasive assessment of genetic variations |
| US10196681B2 (en) | 2011-10-06 | 2019-02-05 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9367663B2 (en) | 2011-10-06 | 2016-06-14 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US8688388B2 (en) | 2011-10-11 | 2014-04-01 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| WO2013059746A1 (en) | 2011-10-19 | 2013-04-25 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
| EP2805280B1 (en) | 2012-01-20 | 2022-10-05 | Sequenom, Inc. | Diagnostic processes that factor experimental conditions |
| EP4372084A3 (en) | 2012-01-26 | 2024-08-14 | Tecan Genomics, Inc. | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
| HK1206069A1 (en) | 2012-02-27 | 2015-12-31 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| WO2013169339A1 (en) | 2012-05-10 | 2013-11-14 | The General Hospital Corporation | Methods for determining a nucleotide sequence |
| US10504613B2 (en) | 2012-12-20 | 2019-12-10 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9920361B2 (en) | 2012-05-21 | 2018-03-20 | Sequenom, Inc. | Methods and compositions for analyzing nucleic acid |
| WO2013177581A2 (en) * | 2012-05-24 | 2013-11-28 | University Of Washington Through Its Center For Commercialization | Whole genome sequencing of a human fetus |
| WO2013191775A2 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US10497461B2 (en) | 2012-06-22 | 2019-12-03 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| CN104685064A (zh) * | 2012-07-24 | 2015-06-03 | 纳特拉公司 | 高度复合pcr方法和组合物 |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| US10876152B2 (en) | 2012-09-04 | 2020-12-29 | Guardant Health, Inc. | Systems and methods to detect rare mutations and copy number variation |
| US11913065B2 (en) | 2012-09-04 | 2024-02-27 | Guardent Health, Inc. | Systems and methods to detect rare mutations and copy number variation |
| EP4424826A3 (en) | 2012-09-04 | 2024-11-27 | Guardant Health, Inc. | Systems and methods to detect rare mutations and copy number variation |
| US10482994B2 (en) | 2012-10-04 | 2019-11-19 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| PL2912468T3 (pl) | 2012-10-29 | 2019-04-30 | Univ Johns Hopkins | Test papanicolaou pod kątem raka jajnika i endometrium |
| US20130309666A1 (en) | 2013-01-25 | 2013-11-21 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US9235808B2 (en) | 2013-03-14 | 2016-01-12 | International Business Machines Corporation | Evaluation of predictions in the absence of a known ground truth |
| US20140274738A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Sequential sequencing |
| ES3042844T3 (en) | 2013-03-15 | 2025-11-24 | Univ Leland Stanford Junior | Identification and use of circulating nucleic acid tumor markers |
| ES2939547T3 (es) | 2013-04-03 | 2023-04-24 | Sequenom Inc | Métodos y procedimientos para la evaluación no invasiva de variaciones genéticas |
| IL300163B2 (en) | 2013-05-24 | 2024-06-01 | Sequenom Inc | Methods and processes for non-invasive assessment of genetic variations |
| MX2015016911A (es) | 2013-06-21 | 2016-06-21 | Sequenom Inc | Metodos y procesos para evaluacion no invasiva de variaciones geneticas. |
| AU2014312208B2 (en) | 2013-08-28 | 2019-07-25 | Becton, Dickinson And Company | Massively parallel single cell analysis |
| US9499870B2 (en) | 2013-09-27 | 2016-11-22 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| US10262755B2 (en) | 2014-04-21 | 2019-04-16 | Natera, Inc. | Detecting cancer mutations and aneuploidy in chromosomal segments |
| US10577655B2 (en) | 2013-09-27 | 2020-03-03 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| IL289974B (en) | 2013-10-04 | 2022-09-01 | Sequenom Inc | Methods and processes for non-invasive evaluation of genetic variations |
| EP3055427B1 (en) | 2013-10-07 | 2018-09-12 | Sequenom, Inc. | Methods and processes for non-invasive assessment of chromosome alterations |
| JP6525473B2 (ja) | 2013-11-13 | 2019-06-05 | ニューゲン テクノロジーズ, インコーポレイテッド | 複製物配列決定リードを同定するための組成物および方法 |
| EP3099820A4 (en) | 2014-01-27 | 2018-01-03 | The General Hospital Corporation | Methods of preparing nucleic acids for sequencing |
| US9745614B2 (en) | 2014-02-28 | 2017-08-29 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
| GB2524948A (en) * | 2014-03-07 | 2015-10-14 | Oxford Gene Technology Operations Ltd | Detecting Increase or Decrease in the Amount of a Nucleic Acid having a Sequence of Interest |
| JP6659575B2 (ja) | 2014-04-21 | 2020-03-04 | ナテラ, インコーポレイテッド | 変異の検出および染色体分節の倍数性 |
| US12492429B2 (en) | 2014-04-21 | 2025-12-09 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| US20180173846A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| EP3940703A1 (en) * | 2014-07-18 | 2022-01-19 | Illumina, Inc. | Non-invasive prenatal diagnosis of fetal genetic conditions using cellular dna and cell free dna |
| EP3760739B1 (en) | 2014-07-30 | 2025-09-03 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| EP3192879A4 (en) * | 2014-09-11 | 2017-07-19 | Fujifilm Corporation | Method for detecting presence/absence of fetal chromosomal aneuploidy |
| ES2925014T3 (es) | 2014-09-12 | 2022-10-13 | Univ Leland Stanford Junior | Identificación y uso de ácidos nucleicos circulantes |
| WO2016049878A1 (zh) * | 2014-09-30 | 2016-04-07 | 深圳华大基因科技有限公司 | 一种基于snp分型的亲子鉴定方法及应用 |
| EP3224382A1 (en) * | 2014-11-24 | 2017-10-04 | Shaare Zedek Medical Center | Fetal haplotype identification |
| EP3239875B1 (en) * | 2014-12-26 | 2019-10-02 | National University Corporation, Tohoku University | Method for determining genotype of particular gene locus group or individual gene locus, determination computer system and determination program |
| CN107208158B (zh) | 2015-02-27 | 2022-01-28 | 贝克顿迪金森公司 | 空间上可寻址的分子条形编码 |
| EP3277843A2 (en) | 2015-03-30 | 2018-02-07 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| WO2016172373A1 (en) | 2015-04-23 | 2016-10-27 | Cellular Research, Inc. | Methods and compositions for whole transcriptome amplification |
| DK3294906T3 (en) | 2015-05-11 | 2024-08-05 | Natera Inc | Methods for determining ploidy |
| CN104946773B (zh) * | 2015-07-06 | 2018-04-13 | 厦门万基生物科技有限公司 | 一种利用snp进行产前亲权关系判定的方法 |
| KR101817785B1 (ko) * | 2015-08-06 | 2018-01-11 | 이원다이애그노믹스(주) | 다양한 플랫폼에서 태아의 성별과 성염색체 이상을 구분할 수 있는 새로운 방법 |
| US11286531B2 (en) | 2015-08-11 | 2022-03-29 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| ES2745694T3 (es) | 2015-09-11 | 2020-03-03 | Cellular Res Inc | Métodos y composiciones para normalización de biblioteca de ácido nucleico |
| EP3375886A4 (en) * | 2015-11-10 | 2018-09-19 | Fujifilm Corporation | Method for determining whether cells or cell masses are originated from same person, or unrelated persons, or parent and child, or related persons |
| CN109477138A (zh) | 2016-04-15 | 2019-03-15 | 纳特拉公司 | 肺癌检测方法 |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| WO2017214557A1 (en) | 2016-06-10 | 2017-12-14 | Counsyl, Inc. | Nucleic acid sequencing adapters and uses thereof |
| CA3030890A1 (en) | 2016-07-27 | 2018-02-01 | Sequenom, Inc. | Genetic copy number alteration classifications |
| US10683531B2 (en) | 2016-09-15 | 2020-06-16 | ArcherDX, Inc. | Methods of nucleic acid sample preparation for analysis of cell-free DNA |
| ES2903357T3 (es) | 2016-09-15 | 2022-04-01 | Archerdx Llc | Métodos de preparación de muestras de ácido nucleico |
| SG11201901733PA (en) | 2016-09-26 | 2019-04-29 | Cellular Res Inc | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| US11854666B2 (en) | 2016-09-29 | 2023-12-26 | Myriad Women's Health, Inc. | Noninvasive prenatal screening using dynamic iterative depth optimization |
| WO2018067517A1 (en) | 2016-10-04 | 2018-04-12 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| US10011870B2 (en) | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
| JP7237003B2 (ja) | 2017-01-24 | 2023-03-10 | セクエノム, インコーポレイテッド | 遺伝子片の評価のための方法およびプロセス |
| US11319583B2 (en) | 2017-02-01 | 2022-05-03 | Becton, Dickinson And Company | Selective amplification using blocking oligonucleotides |
| US10894976B2 (en) | 2017-02-21 | 2021-01-19 | Natera, Inc. | Compositions, methods, and kits for isolating nucleic acids |
| US12421550B2 (en) | 2017-03-17 | 2025-09-23 | Sequenom, Inc. | Methods and processes for assessment of genetic mosaicism |
| CA3057589A1 (en) | 2017-03-24 | 2018-09-27 | Counsyl, Inc. | Copy number variant caller |
| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| CN107012226A (zh) * | 2017-04-20 | 2017-08-04 | 司法部司法鉴定科学技术研究所 | 一种基于高通量测序的snp位点的检测试剂盒及其检测方法 |
| CA3059559A1 (en) | 2017-06-05 | 2018-12-13 | Becton, Dickinson And Company | Sample indexing for single cells |
| CN107217095B (zh) * | 2017-06-15 | 2021-06-04 | 广东腾飞基因科技股份有限公司 | 人类亲权鉴定用多重pcr引物组及检测方法 |
| CN111051511A (zh) | 2017-08-04 | 2020-04-21 | 十亿至一公司 | 用于与生物靶相关的表征的靶相关分子 |
| MX2020001575A (es) | 2017-08-07 | 2020-11-18 | Univ Johns Hopkins | Materiales y métodos para evaluar y tratar el cáncer. |
| CN107557458A (zh) * | 2017-10-11 | 2018-01-09 | 华东医药(杭州)基因科技有限公司 | 一种有效检测基因型的方法及装置 |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| US20200299677A1 (en) | 2017-10-27 | 2020-09-24 | Juno Diagnostics, Inc. | Devices, systems and methods for ultra-low volume liquid biopsy |
| CN108048575B (zh) * | 2017-11-03 | 2021-06-15 | 中南大学湘雅医院 | 一种用于产前无创亲子鉴定的试剂盒及方法 |
| US12084720B2 (en) | 2017-12-14 | 2024-09-10 | Natera, Inc. | Assessing graft suitability for transplantation |
| WO2019161244A1 (en) | 2018-02-15 | 2019-08-22 | Natera, Inc. | Methods for isolating nucleic acids with size selection |
| US12462935B2 (en) | 2018-03-30 | 2025-11-04 | Nucleix Ltd. | Deep learning-based methods, devices, and systems for prenatal testing |
| CN112236535A (zh) | 2018-04-14 | 2021-01-15 | 纳特拉公司 | 用于借助于循环肿瘤dna的个人化检测的癌症检测和监测的方法 |
| CN118910215A (zh) | 2018-05-03 | 2024-11-08 | 贝克顿迪金森公司 | 在相对的转录物末端进行分子条形码化 |
| JP7407128B2 (ja) | 2018-05-03 | 2023-12-28 | ベクトン・ディキンソン・アンド・カンパニー | ハイスループットマルチオミクスサンプル解析 |
| CN108875307B (zh) * | 2018-06-29 | 2021-12-03 | 上海欧易生物医学科技有限公司 | 一种基于孕妇外周血中胎儿游离dna的亲子鉴定方法 |
| US12234509B2 (en) | 2018-07-03 | 2025-02-25 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
| WO2020051542A2 (en) * | 2018-09-07 | 2020-03-12 | Illumina, Inc. | A method to determine if a circulating fetal cell isolated from a pregnant mother is from either the current or a historical pregnancy |
| EP3861134B1 (en) | 2018-10-01 | 2024-09-04 | Becton, Dickinson and Company | Determining 5' transcript sequences |
| CN112969789A (zh) | 2018-11-08 | 2021-06-15 | 贝克顿迪金森公司 | 使用随机引发的单细胞全转录组分析 |
| CN113195717A (zh) | 2018-12-13 | 2021-07-30 | 贝克顿迪金森公司 | 单细胞全转录组分析中的选择性延伸 |
| CN110964690A (zh) * | 2019-01-16 | 2020-04-07 | 北京大学深圳医院 | 辅助生殖技术实施过程中防止配子错配的方法 |
| CN113574178B (zh) | 2019-01-23 | 2024-10-29 | 贝克顿迪金森公司 | 与抗体关联的寡核苷酸 |
| EP3924506A1 (en) | 2019-02-14 | 2021-12-22 | Becton Dickinson and Company | Hybrid targeted and whole transcriptome amplification |
| EP3980559A1 (en) | 2019-06-06 | 2022-04-13 | Natera, Inc. | Methods for detecting immune cell dna and monitoring immune system |
| WO2020257717A1 (en) * | 2019-06-21 | 2020-12-24 | Coopersurgical, Inc. | System and method for determining genetic relationships between a sperm provider, oocyte provider, and the respective conceptus |
| WO2021016239A1 (en) | 2019-07-22 | 2021-01-28 | Becton, Dickinson And Company | Single cell chromatin immunoprecipitation sequencing assay |
| CN112466397A (zh) * | 2019-09-09 | 2021-03-09 | 深圳乐土生物科技有限公司 | 一种用于亲缘关系检测的方法和装置 |
| ES2987640T3 (es) | 2019-11-08 | 2024-11-15 | Becton Dickinson Co | Uso de cebado aleatorio para obtener información de V(D)J de longitud completa para la secuenciación del repertorio inmunitario |
| CN110724732A (zh) * | 2019-11-12 | 2020-01-24 | 陈洪亮 | 一种产前亲权关系鉴定的方法 |
| WO2021137770A1 (en) * | 2019-12-30 | 2021-07-08 | Geneton S.R.O. | Method for fetal fraction estimation based on detection and interpretation of single nucleotide variants |
| CN115244184A (zh) | 2020-01-13 | 2022-10-25 | 贝克顿迪金森公司 | 用于定量蛋白和rna的方法和组合物 |
| EP4097228B1 (en) | 2020-01-29 | 2024-08-14 | Becton, Dickinson and Company | Barcoded wells for spatial mapping of single cells through sequencing |
| CN113275053A (zh) | 2020-02-03 | 2021-08-20 | 帝肯基因组学公司 | 试剂存储系统 |
| US20220073977A1 (en) | 2020-02-14 | 2022-03-10 | The Johns Hopkins University | Methods and materials for assessing nucleic acids |
| CN115151810A (zh) | 2020-02-25 | 2022-10-04 | 贝克顿迪金森公司 | 实现使用单细胞样品作为单色补偿对照的双特异性探针 |
| EP4110953A2 (en) * | 2020-02-28 | 2023-01-04 | Laboratory Corporation of America Holdings | Compositions, methods, and systems for paternity determination |
| CN111518917B (zh) * | 2020-04-02 | 2022-06-07 | 中山大学 | 一种用于无创产前亲权关系判定的微单倍型遗传标记组合及方法 |
| EP4150118A1 (en) | 2020-05-14 | 2023-03-22 | Becton Dickinson and Company | Primers for immune repertoire profiling |
| CN115803445A (zh) | 2020-06-02 | 2023-03-14 | 贝克顿迪金森公司 | 用于5撇基因表达测定的寡核苷酸和珠 |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| CN116194589A (zh) | 2020-07-31 | 2023-05-30 | 贝克顿迪金森公司 | 用于转座酶可及染色质的单细胞测定 |
| CN112053743B (zh) * | 2020-08-31 | 2024-11-22 | 济宁医学院 | 一种亲缘关系鉴定方法、系统及设备 |
| WO2022109343A1 (en) | 2020-11-20 | 2022-05-27 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| CA3202523A1 (en) | 2020-12-02 | 2022-06-09 | Genentech, Inc. | Methods and compositions for neoadjuvant and adjuvant urothelial carcinoma therapy |
| WO2022132206A1 (en) | 2020-12-15 | 2022-06-23 | Becton, Dickinson And Company | Single cell secretome analysis |
| KR20230012033A (ko) | 2020-12-16 | 2023-01-25 | 가부시키가이샤 씨디엔에이 | 다형 좌위 신호의 신뢰성 값의 산출 방법 |
| US20220340971A1 (en) * | 2021-03-23 | 2022-10-27 | Billiontoone, Inc. | Fragment analysis for quantitative diagnostics of biological targets |
| CN113549701B (zh) * | 2021-07-21 | 2022-07-01 | 内蒙古农业大学 | 一种山羊亲子鉴定snp分子标记及其应用 |
| JP2024528932A (ja) | 2021-08-02 | 2024-08-01 | ナテラ, インコーポレイテッド | 妊娠中の女性における新生物を検出するための方法 |
| CN118613594A (zh) | 2021-09-01 | 2024-09-06 | 纳特拉公司 | 用于非侵入性产前测试的方法 |
| CN113889189B (zh) * | 2021-10-14 | 2025-05-16 | 武汉蓝沙医学检验实验室有限公司 | 以生父和母亲dna评估胎儿dna浓度的方法及应用 |
| CN113999900B (zh) * | 2021-10-14 | 2024-02-20 | 武汉蓝沙医学检验实验室有限公司 | 以孕妇游离dna评估胎儿dna浓度的方法及应用 |
| CN113969310B (zh) * | 2021-10-14 | 2024-02-20 | 武汉蓝沙医学检验实验室有限公司 | 胎儿dna浓度的评估方法及应用 |
| CN113981062B (zh) * | 2021-10-14 | 2024-02-20 | 武汉蓝沙医学检验实验室有限公司 | 以非生父和母亲dna评估胎儿dna浓度的方法及应用 |
| JP7331325B1 (ja) | 2022-08-30 | 2023-08-23 | 株式会社seeDNA | 2種以上の検査を実施可能な遺伝学的解析方法 |
| CN116240274A (zh) * | 2022-11-10 | 2023-06-09 | 武汉蓝沙医学检验实验室有限公司 | 无需父本对妊娠胎儿进行亲子鉴定的方法 |
| CN117116359A (zh) * | 2023-08-07 | 2023-11-24 | 天津大学 | Dna存储中基于最大后验概率的鲁棒多序列重建方法 |
| JP7684727B2 (ja) * | 2023-08-22 | 2025-05-28 | 株式会社seeDNA | 非侵襲的出生前親子鑑定方法 |
| CN117487932B (zh) * | 2023-11-22 | 2024-09-03 | 复旦大学 | 一种用于亲子鉴定的snp位点组合、及其检测引物对和应用 |
Family Cites Families (329)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4935342A (en) | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
| SU1552642A3 (ru) * | 1987-12-07 | 1994-03-15 | Институт молекулярной генетики РАН | Способ определения родства живых организмов |
| US5153117A (en) * | 1990-03-27 | 1992-10-06 | Genetype A.G. | Fetal cell recovery method |
| US6582908B2 (en) | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| IL103935A0 (en) | 1991-12-04 | 1993-05-13 | Du Pont | Method for the identification of microorganisms by the utilization of directed and arbitrary dna amplification |
| GB9305984D0 (en) | 1993-03-23 | 1993-05-12 | Royal Free Hosp School Med | Predictive assay |
| WO1995006137A1 (en) | 1993-08-27 | 1995-03-02 | Australian Red Cross Society | Detection of genes |
| SE9400522D0 (sv) | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| US5716776A (en) | 1994-03-04 | 1998-02-10 | Mark H. Bogart | Enrichment by preferential mitosis of fetal lymphocytes from a maternal blood sample |
| US6025128A (en) | 1994-09-29 | 2000-02-15 | The University Of Tulsa | Prediction of prostate cancer progression by analysis of selected predictive parameters |
| US6479235B1 (en) | 1994-09-30 | 2002-11-12 | Promega Corporation | Multiplex amplification of short tandem repeat loci |
| US6720140B1 (en) | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
| US5733729A (en) | 1995-09-14 | 1998-03-31 | Affymetrix, Inc. | Computer-aided probability base calling for arrays of nucleic acid probes on chips |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US6852487B1 (en) | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| US6156504A (en) | 1996-03-15 | 2000-12-05 | The Penn State Research Foundation | Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays |
| US6108635A (en) | 1996-05-22 | 2000-08-22 | Interleukin Genetics, Inc. | Integrated disease information system |
| US6300077B1 (en) | 1996-08-14 | 2001-10-09 | Exact Sciences Corporation | Methods for the detection of nucleic acids |
| US6100029A (en) | 1996-08-14 | 2000-08-08 | Exact Laboratories, Inc. | Methods for the detection of chromosomal aberrations |
| US6221654B1 (en) | 1996-09-25 | 2001-04-24 | California Institute Of Technology | Method and apparatus for analysis and sorting of polynucleotides based on size |
| US5860917A (en) | 1997-01-15 | 1999-01-19 | Chiron Corporation | Method and apparatus for predicting therapeutic outcomes |
| US5824467A (en) | 1997-02-25 | 1998-10-20 | Celtrix Pharmaceuticals | Methods for predicting drug response |
| GB9704444D0 (en) | 1997-03-04 | 1997-04-23 | Isis Innovation | Non-invasive prenatal diagnosis |
| US20010051341A1 (en) | 1997-03-04 | 2001-12-13 | Isis Innovation Limited | Non-invasive prenatal diagnosis |
| ATE280177T1 (de) | 1997-03-20 | 2004-11-15 | Hoffmann La Roche | Modifizierte primer |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| US5994148A (en) | 1997-06-23 | 1999-11-30 | The Regents Of University Of California | Method of predicting and enhancing success of IVF/ET pregnancy |
| US6833242B2 (en) | 1997-09-23 | 2004-12-21 | California Institute Of Technology | Methods for detecting and sorting polynucleotides based on size |
| US6124120A (en) | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
| ATE421583T1 (de) | 1999-04-30 | 2009-02-15 | Cyclops Genome Sciences Ltd | Isolierung von nukleinsäuren |
| US6180349B1 (en) | 1999-05-18 | 2001-01-30 | The Regents Of The University Of California | Quantitative PCR method to enumerate DNA copy number |
| US7058517B1 (en) | 1999-06-25 | 2006-06-06 | Genaissance Pharmaceuticals, Inc. | Methods for obtaining and using haplotype data |
| US6964847B1 (en) | 1999-07-14 | 2005-11-15 | Packard Biosciences Company | Derivative nucleic acids and uses thereof |
| GB9917307D0 (en) | 1999-07-23 | 1999-09-22 | Sec Dep Of The Home Department | Improvements in and relating to analysis of DNA |
| US6440706B1 (en) | 1999-08-02 | 2002-08-27 | Johns Hopkins University | Digital amplification |
| US6251604B1 (en) | 1999-08-13 | 2001-06-26 | Genopsys, Inc. | Random mutagenesis and amplification of nucleic acid |
| US6221603B1 (en) | 2000-02-04 | 2001-04-24 | Molecular Dynamics, Inc. | Rolling circle amplification assay for nucleic acid analysis |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US7510834B2 (en) | 2000-04-13 | 2009-03-31 | Hidetoshi Inoko | Gene mapping method using microsatellite genetic polymorphism markers |
| GB0009179D0 (en) | 2000-04-13 | 2000-05-31 | Imp College Innovations Ltd | Non-invasive prenatal diagnosis |
| AU2001274869A1 (en) | 2000-05-20 | 2001-12-03 | The Regents Of The University Of Michigan | Method of producing a dna library using positional amplification |
| JP2004511210A (ja) | 2000-05-23 | 2004-04-15 | バリアジェニックス インコーポレーテッド | 配列変動を検出する、dnaを遺伝分析するための方法 |
| AU2001255518A1 (en) | 2000-06-07 | 2001-12-17 | Baylor College Of Medicine | Compositions and methods for array-based nucleic acid hybridization |
| US7058616B1 (en) | 2000-06-08 | 2006-06-06 | Virco Bvba | Method and system for predicting resistance of a disease to a therapeutic agent using a neural network |
| GB0016742D0 (en) | 2000-07-10 | 2000-08-30 | Simeg Limited | Diagnostic method |
| US20020107640A1 (en) | 2000-11-14 | 2002-08-08 | Ideker Trey E. | Methods for determining the true signal of an analyte |
| EP1368369A4 (en) | 2000-11-15 | 2006-02-22 | Hoffmann La Roche | METHODS AND REAGENTS FOR IDENTIFYING RARE EMBRYONIC CELLS IN THE MATERNAL CIRCULATORY SYSTEM |
| US7218764B2 (en) | 2000-12-04 | 2007-05-15 | Cytokinetics, Inc. | Ploidy classification method |
| WO2002073504A1 (en) | 2001-03-14 | 2002-09-19 | Gene Logic, Inc. | A system and method for retrieving and using gene expression data from multiple sources |
| AU2002238994A1 (en) | 2001-03-23 | 2002-10-08 | Center For Advanced Science And Technology Incubation, Ltd. | Mononucleosome and process for producing the same, method of assaying antibody specific to nucleosome, method of diagnosing autoimmune disease, process for producing nucleosome dna, dna plate, process for producing dna plate and method of assaying anti-dna antibody |
| US6489135B1 (en) | 2001-04-17 | 2002-12-03 | Atairgintechnologies, Inc. | Determination of biological characteristics of embryos fertilized in vitro by assaying for bioactive lipids in culture media |
| FR2824144B1 (fr) | 2001-04-30 | 2004-09-17 | Metagenex S A R L | Methode de diagnostic prenatal sur cellule foetale isolee du sang maternel |
| WO2002087431A1 (en) | 2001-05-01 | 2002-11-07 | Structural Bioinformatics, Inc. | Diagnosing inapparent diseases from common clinical tests using bayesian analysis |
| US7262030B2 (en) | 2001-05-09 | 2007-08-28 | Virginia Commonwealth University | Multiple sequencible and ligatible structures for genomic analysis |
| US20030101000A1 (en) | 2001-07-24 | 2003-05-29 | Bader Joel S. | Family based tests of association using pooled DNA and SNP markers |
| US6958211B2 (en) | 2001-08-08 | 2005-10-25 | Tibotech Bvba | Methods of assessing HIV integrase inhibitor therapy |
| US6807491B2 (en) | 2001-08-30 | 2004-10-19 | Hewlett-Packard Development Company, L.P. | Method and apparatus for combining gene predictions using bayesian networks |
| AUPR749901A0 (en) | 2001-09-06 | 2001-09-27 | Monash University | Method of identifying chromosomal abnormalities and prenatal diagnosis |
| US8986944B2 (en) | 2001-10-11 | 2015-03-24 | Aviva Biosciences Corporation | Methods and compositions for separating rare cells from fluid samples |
| EP1442139A4 (en) | 2001-10-12 | 2005-01-26 | Univ Queensland | SELECTION AND AMPLIFICATION OF MULTIPLE GENETIC MARKERS |
| US7297485B2 (en) | 2001-10-15 | 2007-11-20 | Qiagen Gmbh | Method for nucleic acid amplification that results in low amplification bias |
| US20030119004A1 (en) | 2001-12-05 | 2003-06-26 | Wenz H. Michael | Methods for quantitating nucleic acids using coupled ligation and amplification |
| CN100468058C (zh) | 2001-12-11 | 2009-03-11 | 株式会社奈特克 | 血细胞分离体系 |
| WO2003062441A1 (en) | 2002-01-18 | 2003-07-31 | Genzyme Corporation | Methods for fetal dna detection and allele quantitation |
| CA2474982A1 (en) | 2002-02-01 | 2003-08-07 | Rosetta Inpharmatics Llc | Computer systems and methods for identifying genes and determining pathways associated with traits |
| CA2477611A1 (en) | 2002-03-01 | 2003-09-12 | Ravgen, Inc. | Rapid analysis of variations in a genome |
| US6977162B2 (en) | 2002-03-01 | 2005-12-20 | Ravgen, Inc. | Rapid analysis of variations in a genome |
| US20060229823A1 (en) | 2002-03-28 | 2006-10-12 | Affymetrix, Inc. | Methods and computer software products for analyzing genotyping data |
| CA2484360A1 (en) | 2002-05-02 | 2003-11-13 | The University Of North Carolina At Chapel Hill | In vitro mutagenesis, phenotyping, and gene mapping |
| US7442506B2 (en) | 2002-05-08 | 2008-10-28 | Ravgen, Inc. | Methods for detection of genetic disorders |
| US20070178478A1 (en) | 2002-05-08 | 2007-08-02 | Dhallan Ravinder S | Methods for detection of genetic disorders |
| US7727720B2 (en) | 2002-05-08 | 2010-06-01 | Ravgen, Inc. | Methods for detection of genetic disorders |
| US20040229231A1 (en) | 2002-05-28 | 2004-11-18 | Frudakis Tony N. | Compositions and methods for inferring ancestry |
| CA2492631C (en) | 2002-05-31 | 2013-03-12 | Genetic Technologies Limited | Maternal antibodies as fetal cell markers to identify and enrich fetal cells from maternal blood |
| WO2003106623A2 (en) | 2002-06-13 | 2003-12-24 | New York University | Early noninvasive prenatal test for aneuploidies and heritable conditions |
| US20050009069A1 (en) | 2002-06-25 | 2005-01-13 | Affymetrix, Inc. | Computer software products for analyzing genotyping |
| EP1388812A1 (en) | 2002-07-04 | 2004-02-11 | Ronald E. Dr. Kates | Method for training a learning-capable system |
| US20040117346A1 (en) | 2002-09-20 | 2004-06-17 | Kilian Stoffel | Computer-based method and apparatus for repurposing an ontology |
| US7459273B2 (en) | 2002-10-04 | 2008-12-02 | Affymetrix, Inc. | Methods for genotyping selected polymorphism |
| WO2004033649A2 (en) | 2002-10-07 | 2004-04-22 | University Of Medicine And Dentistry Of New Jersey | High throughput multiplex dna sequence amplifications |
| AU2003291481A1 (en) | 2002-11-11 | 2004-06-03 | Affymetrix, Inc. | Methods for identifying dna copy number changes |
| DE602004021902D1 (de) | 2003-01-17 | 2009-08-20 | Univ Boston | Haplotypanalyse |
| WO2004065628A1 (en) | 2003-01-21 | 2004-08-05 | Guoliang Fu | Quantitative multiplex detection of nucleic acids |
| AU2004209426B2 (en) | 2003-01-29 | 2008-04-17 | 454 Life Sciences Corporation | Method for preparing single-stranded DNA libraries |
| US20040209299A1 (en) | 2003-03-07 | 2004-10-21 | Rubicon Genomics, Inc. | In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA |
| US20040197832A1 (en) | 2003-04-03 | 2004-10-07 | Mor Research Applications Ltd. | Non-invasive prenatal genetic diagnosis using transcervical cells |
| JP2004348437A (ja) | 2003-05-22 | 2004-12-09 | Matsushita Electric Ind Co Ltd | リソース管理装置、リソース管理方法及び記録媒体 |
| CN101410008A (zh) | 2003-05-28 | 2009-04-15 | 先锋高级育种国际公司 | 植物育种方法 |
| US20040259100A1 (en) | 2003-06-20 | 2004-12-23 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| ATE425265T1 (de) | 2003-07-31 | 2009-03-15 | Sequenom Inc | Verfahren für multiplex- polymerasekettenreaktionen auf hohem niveau und homogenen massenverlängerungsreaktionen zur genotypisierung von polymorphismen |
| WO2005021793A1 (en) | 2003-08-29 | 2005-03-10 | Pantarhei Bioscience B.V. | Prenatal diagnosis of down syndrome by detection of fetal rna markers in maternal blood |
| EP1664077B1 (en) | 2003-09-05 | 2016-04-13 | Trustees of Boston University | Method for non-invasive prenatal diagnosis |
| US20050053950A1 (en) | 2003-09-08 | 2005-03-10 | Enrique Zudaire Ubani | Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons |
| WO2005028645A1 (ja) | 2003-09-24 | 2005-03-31 | Kyushu Tlo Company, Limited | MDR1遺伝子の5’制御領域におけるSNPs |
| WO2005035725A2 (en) | 2003-10-08 | 2005-04-21 | The Trustees Of Boston University | Methods for prenatal diagnosis of chromosomal abnormalities |
| CA2482097C (en) | 2003-10-13 | 2012-02-21 | F. Hoffmann-La Roche Ag | Methods for isolating nucleic acids |
| DE60328193D1 (de) * | 2003-10-16 | 2009-08-13 | Sequenom Inc | Nicht invasiver Nachweis fötaler genetischer Merkmale |
| US20050221341A1 (en) | 2003-10-22 | 2005-10-06 | Shimkets Richard A | Sequence-based karyotyping |
| WO2005044086A2 (en) | 2003-10-30 | 2005-05-19 | Tufts-New England Medical Center | Prenatal diagnosis using cell-free fetal dna in amniotic fluid |
| WO2005071078A1 (en) | 2004-01-12 | 2005-08-04 | Nimblegen Systems Inc. | Method of performing pcr amplification on a microarray |
| US20100216153A1 (en) | 2004-02-27 | 2010-08-26 | Helicos Biosciences Corporation | Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities |
| US7035740B2 (en) | 2004-03-24 | 2006-04-25 | Illumina, Inc. | Artificial intelligence and global normalization methods for genotyping |
| JP4437050B2 (ja) | 2004-03-26 | 2010-03-24 | 株式会社日立製作所 | 診断支援システム、診断支援方法および診断支援サービスの提供方法 |
| WO2005094363A2 (en) | 2004-03-30 | 2005-10-13 | New York University | System, method and software arrangement for bi-allele haplotype phasing |
| EP1732951B1 (en) | 2004-03-31 | 2009-12-02 | Adnagen AG | Monoclonal antibodies with specificity for fetal erythroid cells |
| US7414118B1 (en) | 2004-04-14 | 2008-08-19 | Applied Biosystems Inc. | Modified oligonucleotides and applications thereof |
| US7468249B2 (en) | 2004-05-05 | 2008-12-23 | Biocept, Inc. | Detection of chromosomal disorders |
| US7709194B2 (en) | 2004-06-04 | 2010-05-04 | The Chinese University Of Hong Kong | Marker for prenatal diagnosis and monitoring |
| WO2005123779A2 (en) | 2004-06-14 | 2005-12-29 | The Board Of Trustees Of The University Of Illinois | Antibodies binding to cd34+/cd36+ fetal but not to adult cells |
| WO2006002491A1 (en) | 2004-07-06 | 2006-01-12 | Genera Biosystems Pty Ltd | Method of detecting aneuploidy |
| DE102004036285A1 (de) | 2004-07-27 | 2006-02-16 | Advalytix Ag | Verfahren zum Bestimmen der Häufigkeit von Sequenzen einer Probe |
| WO2006020617A1 (en) | 2004-08-09 | 2006-02-23 | Generation Biotech, Llc | Method for nucleic acid isolation and amplification |
| JP2008511058A (ja) | 2004-08-18 | 2008-04-10 | アボツト・モレキユラー・インコーポレイテツド | コンピュータシステムを用いるデータ品質および/または部分異数染色体の決定 |
| US8024128B2 (en) | 2004-09-07 | 2011-09-20 | Gene Security Network, Inc. | System and method for improving clinical decisions by aggregating, validating and analysing genetic and phenotypic data |
| US20060088574A1 (en) | 2004-10-25 | 2006-04-27 | Manning Paul B | Nutritional supplements |
| US20060134662A1 (en) | 2004-10-25 | 2006-06-22 | Pratt Mark R | Method and system for genotyping samples in a normalized allelic space |
| EP1828419A1 (en) | 2004-11-17 | 2007-09-05 | ReproCure, LLC | Methods of determining human egg competency |
| US20070042384A1 (en) | 2004-12-01 | 2007-02-22 | Weiwei Li | Method for isolating and modifying DNA from blood and body fluids |
| US20090098534A1 (en) | 2005-02-25 | 2009-04-16 | The Regents Of The University Of California | Full Karyotype Single Cell Chromosome Analysis |
| US7618777B2 (en) | 2005-03-16 | 2009-11-17 | Agilent Technologies, Inc. | Composition and method for array hybridization |
| TWI367260B (en) | 2005-03-18 | 2012-07-01 | Univ Hong Kong Chinese | Method for detecting the presence of a fetus with trisomy 21 in a pregnant woman and kit thereof |
| EP1859050B1 (en) | 2005-03-18 | 2012-10-24 | The Chinese University Of Hong Kong | A method for the detection of chromosomal aneuploidies |
| AU2006341607B2 (en) | 2005-05-31 | 2011-03-17 | Applied Biosystems, Llc. | Multiplexed amplification of short nucleic acids |
| EP3257949A1 (en) | 2005-06-15 | 2017-12-20 | Complete Genomics Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
| US20070020640A1 (en) | 2005-07-21 | 2007-01-25 | Mccloskey Megan L | Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis |
| RU2290078C1 (ru) | 2005-07-25 | 2006-12-27 | Евгений Владимирович Новичков | Способ прогнозирования рецидива серозного рака яичников |
| US10083273B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US20070178501A1 (en) | 2005-12-06 | 2007-08-02 | Matthew Rabinowitz | System and method for integrating and validating genotypic, phenotypic and medical information into a database according to a standardized ontology |
| US8532930B2 (en) | 2005-11-26 | 2013-09-10 | Natera, Inc. | Method for determining the number of copies of a chromosome in the genome of a target individual using genetic data from genetically related individuals |
| US8515679B2 (en) | 2005-12-06 | 2013-08-20 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
| US10081839B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US20070027636A1 (en) | 2005-07-29 | 2007-02-01 | Matthew Rabinowitz | System and method for using genetic, phentoypic and clinical data to make predictions for clinical or lifestyle decisions |
| ATE510930T1 (de) | 2005-08-02 | 2011-06-15 | Rubicon Genomics Inc | Zusammensetzungen und verfahren zur bearbeitung und amplifikation von dna mit verwendung mehrerer enzyme in einer einzigen reaktion |
| GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| US20090228299A1 (en) | 2005-11-09 | 2009-09-10 | The Regents Of The University Of California | Methods and apparatus for context-sensitive telemedicine |
| GB0523276D0 (en) | 2005-11-15 | 2005-12-21 | London Bridge Fertility | Chromosomal analysis by molecular karyotyping |
| EP3373175B1 (en) | 2005-11-26 | 2025-05-28 | Natera, Inc. | System and method for cleaning noisy genetic data and using data to make predictions |
| WO2007070482A2 (en) | 2005-12-14 | 2007-06-21 | Xueliang Xia | Microarray-based preimplantation genetic diagnosis of chromosomal abnormalities |
| EP2385143B1 (en) | 2006-02-02 | 2016-07-13 | The Board of Trustees of the Leland Stanford Junior University | Non-invasive fetal genetic screening by digital analysis |
| WO2007092538A2 (en) | 2006-02-07 | 2007-08-16 | President And Fellows Of Harvard College | Methods for making nucleotide probes for sequencing and synthesis |
| WO2007091064A1 (en) | 2006-02-08 | 2007-08-16 | Solexa Limited | End modification to prevent over-representation of fragments |
| SI2351858T1 (sl) | 2006-02-28 | 2015-06-30 | University Of Louisville Research Foundation Med Center Three, | Zaznavanje fetalnih kromosomskih nenormalnosti z uporabo tandema polimorfizmov posameznih nukleotidov |
| CA2646254A1 (en) | 2006-03-13 | 2007-09-20 | Veridex, Llc | Propagation of primary cells |
| WO2007112418A2 (en) | 2006-03-28 | 2007-10-04 | Baylor College Of Medicine | Screening for down syndrome |
| WO2007121276A2 (en) | 2006-04-12 | 2007-10-25 | Biocept, Inc. | Enrichment of circulating fetal dna |
| US7901884B2 (en) | 2006-05-03 | 2011-03-08 | The Chinese University Of Hong Kong | Markers for prenatal diagnosis and monitoring |
| US7702468B2 (en) | 2006-05-03 | 2010-04-20 | Population Diagnostics, Inc. | Evaluating genetic disorders |
| US8137912B2 (en) | 2006-06-14 | 2012-03-20 | The General Hospital Corporation | Methods for the diagnosis of fetal abnormalities |
| EP4108780A1 (en) | 2006-06-14 | 2022-12-28 | Verinata Health, Inc. | Rare cell analysis using sample splitting and dna tags |
| WO2007147074A2 (en) | 2006-06-14 | 2007-12-21 | Living Microsystems, Inc. | Use of highly parallel snp genotyping for fetal diagnosis |
| EP2589668A1 (en) | 2006-06-14 | 2013-05-08 | Verinata Health, Inc | Rare cell analysis using sample splitting and DNA tags |
| WO2009035447A1 (en) | 2006-06-14 | 2009-03-19 | Living Microsystems, Inc. | Diagnosis of fetal abnormalities by comparative genomic hybridization analysis |
| US20080124721A1 (en) | 2006-06-14 | 2008-05-29 | Martin Fuchs | Analysis of rare cell-enriched samples |
| EP4170042A1 (en) | 2006-06-14 | 2023-04-26 | Verinata Health, Inc. | Methods for the diagnosis of fetal abnormalities |
| EP2035540A2 (en) | 2006-06-15 | 2009-03-18 | Stratagene | System for isolating biomolecules from a sample |
| EP2038426A2 (en) | 2006-06-16 | 2009-03-25 | Sequenom, Inc. | Methods and compositions for the amplification, detection and quantification of nucleic acid from a sample |
| US20080182244A1 (en) | 2006-08-04 | 2008-07-31 | Ikonisys, Inc. | Pre-Implantation Genetic Diagnosis Test |
| CA2661640A1 (en) | 2006-08-24 | 2008-02-28 | University Of Massachusetts Medical School | Mapping of genomic interactions |
| CA2663029C (en) | 2006-09-14 | 2016-07-19 | Ibis Biosciences, Inc. | Targeted whole genome amplification method for identification of pathogens |
| US20080085836A1 (en) | 2006-09-22 | 2008-04-10 | Kearns William G | Method for genetic testing of human embryos for chromosome abnormalities, segregating genetic disorders with or without a known mutation and mitochondrial disorders following in vitro fertilization (IVF), embryo culture and embryo biopsy |
| AU2007311126A1 (en) | 2006-10-16 | 2008-04-24 | Celula Inc. | Methods and compositions for differential expansion of fetal cells in maternal blood and their use |
| WO2008051928A2 (en) | 2006-10-23 | 2008-05-02 | The Salk Institute For Biological Studies | Target-oriented whole genome amplification of nucliec acids |
| WO2008059578A1 (en) | 2006-11-16 | 2008-05-22 | Olympus Corporation | Multiplex pcr method |
| WO2008081451A2 (en) * | 2007-01-03 | 2008-07-10 | Monaliza Medical Ltd. | Methods and kits for analyzing genetic material of a fetus |
| AU2008213634B2 (en) | 2007-02-08 | 2013-09-05 | Sequenom, Inc. | Nucleic acid-based tests for RhD typing, gender determination and nucleic acid quantification |
| WO2008115497A2 (en) | 2007-03-16 | 2008-09-25 | Gene Security Network | System and method for cleaning noisy genetic data and determining chromsome copy number |
| ITTO20070307A1 (it) | 2007-05-04 | 2008-11-05 | Silicon Biosystems Spa | Metodo e dispositivo per la diagnosi prenatale non-invasiva |
| KR20100038330A (ko) | 2007-05-31 | 2010-04-14 | 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 | 입체구조적 방해 및 효소 관련 신호 증폭 기반의 고 특이성 및 고 민감도 검출 |
| WO2008157264A2 (en) | 2007-06-15 | 2008-12-24 | Sequenom, Inc. | Combined methods for the detection of chromosomal aneuploidy |
| US20090023190A1 (en) | 2007-06-20 | 2009-01-22 | Kai Qin Lao | Sequence amplification with loopable primers |
| CN101842494B (zh) | 2007-07-03 | 2013-05-29 | 吉纳珀莱有限公司 | 用于改进的核酸扩增反应的嵌合引物 |
| WO2009009769A2 (en) | 2007-07-11 | 2009-01-15 | Artemis Health, Inc. | Diagnosis of fetal abnormalities using nucleated red blood cells |
| US12180549B2 (en) | 2007-07-23 | 2024-12-31 | The Chinese University Of Hong Kong | Diagnosing fetal chromosomal aneuploidy using genomic sequencing |
| CN106834481A (zh) | 2007-07-23 | 2017-06-13 | 香港中文大学 | 用于分析遗传变异的方法 |
| US20090053719A1 (en) | 2007-08-03 | 2009-02-26 | The Chinese University Of Hong Kong | Analysis of nucleic acids by digital pcr |
| EP2191276B1 (en) | 2007-08-03 | 2013-11-20 | DKFZ Deutsches Krebsforschungszentrum | Method for prenatal diagnosis using exosomes and cd24 as a marker |
| WO2009032779A2 (en) | 2007-08-29 | 2009-03-12 | Sequenom, Inc. | Methods and compositions for the size-specific seperation of nucleic acid from a sample |
| US9404150B2 (en) | 2007-08-29 | 2016-08-02 | Sequenom, Inc. | Methods and compositions for universal size-specific PCR |
| US8748100B2 (en) | 2007-08-30 | 2014-06-10 | The Chinese University Of Hong Kong | Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences |
| BRPI0816393A2 (pt) | 2007-09-07 | 2015-03-03 | Fluidigm Corp | Método para determinar o número de cópias relativo de uma sequência de polinucleotídeo alvo em um genoma de um indivíduo |
| EP2191012A1 (en) | 2007-09-21 | 2010-06-02 | Streck, Inc. | Nucleic acid isolation in preserved whole blood |
| EP2201143B2 (en) | 2007-09-21 | 2016-08-24 | Katholieke Universiteit Leuven | Tools and methods for genetic tests using next generation sequencing |
| JP5726529B2 (ja) | 2007-11-30 | 2015-06-03 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | 単一試料からのゲノムdna、rnaおよびタンパク質の単離方法 |
| EP2077337A1 (en) | 2007-12-26 | 2009-07-08 | Eppendorf Array Technologies SA | Amplification and detection composition, method and kit |
| EP2620511B1 (en) | 2008-01-17 | 2018-02-28 | Sequenom, Inc. | Single molecule nucleic acid sequence analysis processes |
| WO2009092035A2 (en) | 2008-01-17 | 2009-07-23 | Sequenom, Inc. | Methods and compositions for the analysis of biological molecules |
| WO2009105531A1 (en) | 2008-02-19 | 2009-08-27 | Gene Security Network, Inc. | Methods for cell genotyping |
| US20090221620A1 (en) * | 2008-02-20 | 2009-09-03 | Celera Corporation | Gentic polymorphisms associated with stroke, methods of detection and uses thereof |
| CA2717320A1 (en) | 2008-03-11 | 2009-09-17 | Sequenom, Inc. | Nucleic acid-based tests for prenatal gender determination |
| PT2271767T (pt) | 2008-04-03 | 2016-08-18 | Cb Biotechnologies Inc | Reação em cadeia de polimerase para captura de amplicões em multiplexo para amplificação de alvos múltiplos |
| US20110092763A1 (en) | 2008-05-27 | 2011-04-21 | Gene Security Network, Inc. | Methods for Embryo Characterization and Comparison |
| EP2128169A1 (de) | 2008-05-30 | 2009-12-02 | Qiagen GmbH | Verfahren zur Isolierung von kurzkettigen Nukleinsäuren |
| EP2321642B1 (en) | 2008-08-04 | 2017-01-11 | Natera, Inc. | Methods for allele calling and ploidy calling |
| WO2010027870A2 (en) | 2008-08-26 | 2010-03-11 | Fluidigm Corporation | Assay methods for increased throughput of samples and/or targets |
| DE102008045705A1 (de) | 2008-09-04 | 2010-04-22 | Macherey, Nagel Gmbh & Co. Kg Handelsgesellschaft | Verfahren zur Gewinnung von kurzer RNA sowie Kit hierfür |
| US8586310B2 (en) | 2008-09-05 | 2013-11-19 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| EP2324131A4 (en) | 2008-09-17 | 2013-02-27 | Ge Healthcare Bio Sciences | PROCESS FOR ISOLATING SMALL RNA |
| ES2620294T3 (es) | 2008-09-20 | 2017-06-28 | The Board Of Trustees Of The Leland Stanford Junior University | Diagnóstico no invasivo de la aneuploidia fetal por secuenciación |
| CA2748030A1 (en) | 2008-12-22 | 2010-07-01 | Arnold R. Oliphant | Methods and genotyping panels for detecting alleles, genomes, and transcriptomes |
| US11634747B2 (en) | 2009-01-21 | 2023-04-25 | Streck Llc | Preservation of fetal nucleic acids in maternal plasma |
| US8450063B2 (en) | 2009-01-28 | 2013-05-28 | Fluidigm Corporation | Determination of copy number differences by amplification |
| NO2398912T3 (enExample) | 2009-02-18 | 2018-02-10 | ||
| WO2010115044A2 (en) | 2009-04-02 | 2010-10-07 | Fluidigm Corporation | Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation |
| WO2010127186A1 (en) | 2009-04-30 | 2010-11-04 | Prognosys Biosciences, Inc. | Nucleic acid constructs and methods of use |
| KR20110006112A (ko) | 2009-07-13 | 2011-01-20 | 삼성전자주식회사 | 카메라 시스템에서 디스플레이 패널의 백라이트를 제어하는 장치 및 그 방법 |
| US8563242B2 (en) | 2009-08-11 | 2013-10-22 | The Chinese University Of Hong Kong | Method for detecting chromosomal aneuploidy |
| AU2010298620B2 (en) | 2009-09-24 | 2016-08-11 | Qiagen Gaithersburg Inc. | Compositions, methods, and kits for isolating and analyzing nucleic acids using an anion exchange material |
| EP2854056A3 (en) | 2009-09-30 | 2015-06-03 | Natera, Inc. | Methods for non-invasive pre-natal ploidy calling |
| EP2496717B1 (en) | 2009-11-05 | 2017-06-07 | The Chinese University of Hong Kong | Fetal genomic analysis from a maternal biological sample |
| DK2496720T3 (da) | 2009-11-06 | 2020-09-28 | Univ Leland Stanford Junior | Ikke-invasiv diagnose af transplantatafstødning i organ-transplanterede patienter |
| WO2011066476A1 (en) | 2009-11-25 | 2011-06-03 | Quantalife, Inc. | Methods and compositions for detecting genetic material |
| MA33899B1 (fr) | 2009-12-08 | 2013-01-02 | Hemaquest Pharmaceuticals Inc | Procedes et regimes a faible dose pour des troubles des globules rouges |
| US9315857B2 (en) | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US8574842B2 (en) | 2009-12-22 | 2013-11-05 | The Board Of Trustees Of The Leland Stanford Junior University | Direct molecular diagnosis of fetal aneuploidy |
| US9926593B2 (en) | 2009-12-22 | 2018-03-27 | Sequenom, Inc. | Processes and kits for identifying aneuploidy |
| US10388403B2 (en) | 2010-01-19 | 2019-08-20 | Verinata Health, Inc. | Analyzing copy number variation in the detection of cancer |
| WO2011090556A1 (en) | 2010-01-19 | 2011-07-28 | Verinata Health, Inc. | Methods for determining fraction of fetal nucleic acid in maternal samples |
| AU2010343276B2 (en) * | 2010-01-19 | 2015-05-28 | Verinata Health, Inc. | Methods for determining fraction of fetal nucleic acid in maternal samples |
| US9323888B2 (en) | 2010-01-19 | 2016-04-26 | Verinata Health, Inc. | Detecting and classifying copy number variation |
| US20110312503A1 (en) | 2010-01-23 | 2011-12-22 | Artemis Health, Inc. | Methods of fetal abnormality detection |
| US20140206552A1 (en) | 2010-05-18 | 2014-07-24 | Natera, Inc. | Methods for preimplantation genetic diagnosis by sequencing |
| AU2011255641A1 (en) | 2010-05-18 | 2012-12-06 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US20130196862A1 (en) | 2010-05-18 | 2013-08-01 | Natera, Inc. | Informatics Enhanced Analysis of Fetal Samples Subject to Maternal Contamination |
| US20130123120A1 (en) | 2010-05-18 | 2013-05-16 | Natera, Inc. | Highly Multiplex PCR Methods and Compositions |
| US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US20190309358A1 (en) | 2010-05-18 | 2019-10-10 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US12152275B2 (en) | 2010-05-18 | 2024-11-26 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11339429B2 (en) | 2010-05-18 | 2022-05-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US20190284623A1 (en) | 2010-05-18 | 2019-09-19 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11332785B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| WO2013052557A2 (en) | 2011-10-03 | 2013-04-11 | Natera, Inc. | Methods for preimplantation genetic diagnosis by sequencing |
| US20110301854A1 (en) | 2010-06-08 | 2011-12-08 | Curry Bo U | Method of Determining Allele-Specific Copy Number of a SNP |
| US20120034603A1 (en) | 2010-08-06 | 2012-02-09 | Tandem Diagnostics, Inc. | Ligation-based detection of genetic variants |
| US8700338B2 (en) | 2011-01-25 | 2014-04-15 | Ariosa Diagnosis, Inc. | Risk calculation for evaluation of fetal aneuploidy |
| EP2426217A1 (en) | 2010-09-03 | 2012-03-07 | Centre National de la Recherche Scientifique (CNRS) | Analytical methods for cell free nucleic acids and applications |
| CN103534591B (zh) | 2010-10-26 | 2016-04-06 | 利兰·斯坦福青年大学托管委员会 | 通过测序分析进行的非侵入性胎儿遗传筛选 |
| KR102040307B1 (ko) | 2010-11-30 | 2019-11-27 | 더 차이니즈 유니버시티 오브 홍콩 | 암과 연관된 유전적 또는 분자적 이상들의 검출 |
| WO2012078792A2 (en) | 2010-12-07 | 2012-06-14 | Stanford University | Non-invasive determination of fetal inheritance of parental haplotypes at the genome-wide scale |
| WO2012083250A2 (en) | 2010-12-17 | 2012-06-21 | Celula, Inc. | Methods for screening and diagnosing genetic conditions |
| WO2012088456A2 (en) | 2010-12-22 | 2012-06-28 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| US20120190020A1 (en) | 2011-01-25 | 2012-07-26 | Aria Diagnostics, Inc. | Detection of genetic abnormalities |
| JP6153874B2 (ja) | 2011-02-09 | 2017-06-28 | ナテラ, インコーポレイテッド | 非侵襲的出生前倍数性呼び出しのための方法 |
| EP3187597B1 (en) | 2011-02-09 | 2020-06-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| GB2488358A (en) | 2011-02-25 | 2012-08-29 | Univ Plymouth | Enrichment of foetal DNA in maternal plasma |
| WO2012129363A2 (en) | 2011-03-24 | 2012-09-27 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| PT3456844T (pt) | 2011-04-12 | 2020-08-25 | Verinata Health Inc | Resolução de frações de genoma utilizando contagens de polimorfismos |
| CA2833165A1 (en) | 2011-04-14 | 2012-10-18 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| US9411937B2 (en) | 2011-04-15 | 2016-08-09 | Verinata Health, Inc. | Detecting and classifying copy number variation |
| CN103748236B (zh) | 2011-04-15 | 2018-12-25 | 约翰·霍普金斯大学 | 安全测序系统 |
| US9957558B2 (en) | 2011-04-28 | 2018-05-01 | Life Technologies Corporation | Methods and compositions for multiplex PCR |
| EP2546361B1 (en) | 2011-07-11 | 2015-06-03 | Samsung Electronics Co., Ltd. | Method of amplifying target nucleic acid with reduced amplification bias and method for determining relative amount of target nucleic acid in sample |
| US20130024127A1 (en) | 2011-07-19 | 2013-01-24 | John Stuelpnagel | Determination of source contributions using binomial probability calculations |
| GB201115095D0 (en) | 2011-09-01 | 2011-10-19 | Singapore Volition Pte Ltd | Method for detecting nucleosomes containing nucleotides |
| US8712697B2 (en) | 2011-09-07 | 2014-04-29 | Ariosa Diagnostics, Inc. | Determination of copy number variations using binomial probability calculations |
| JP5536729B2 (ja) | 2011-09-20 | 2014-07-02 | 株式会社ソニー・コンピュータエンタテインメント | 情報処理装置、アプリケーション提供システム、アプリケーション提供サーバ、アプリケーション提供方法、および情報処理方法 |
| CN113355321A (zh) | 2011-09-26 | 2021-09-07 | 凯杰有限公司 | 用于分离细胞外核酸的快速方法 |
| US9984198B2 (en) | 2011-10-06 | 2018-05-29 | Sequenom, Inc. | Reducing sequence read count error in assessment of complex genetic variations |
| AU2012321053B2 (en) | 2011-10-07 | 2018-04-19 | Murdoch Childrens Research Institute | Diagnostic assay for tissue transplantation status |
| WO2013078470A2 (en) | 2011-11-22 | 2013-05-30 | MOTIF, Active | Multiplex isolation of protein-associated nucleic acids |
| WO2013086464A1 (en) | 2011-12-07 | 2013-06-13 | The Broad Institute, Inc. | Markers associated with chronic lymphocytic leukemia prognosis and progression |
| US20130190653A1 (en) | 2012-01-25 | 2013-07-25 | Angel Gabriel Alvarez Ramos | Device for blood collection from the placenta and the umbilical cord |
| EP2820155B1 (en) | 2012-02-28 | 2017-07-26 | Population Genetics Technologies Ltd. | Method for attaching a counter sequence to a nucleic acid sample |
| WO2013130848A1 (en) | 2012-02-29 | 2013-09-06 | Natera, Inc. | Informatics enhanced analysis of fetal samples subject to maternal contamination |
| US9862995B2 (en) | 2012-03-13 | 2018-01-09 | Abhijit Ajit Patel | Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing |
| US10385396B2 (en) | 2012-04-19 | 2019-08-20 | The Medical College Of Wisconsin, Inc. | Highly sensitive surveillance using detection of cell free DNA |
| WO2013169339A1 (en) | 2012-05-10 | 2013-11-14 | The General Hospital Corporation | Methods for determining a nucleotide sequence |
| CN109082462B (zh) | 2012-05-21 | 2022-10-28 | 斯克利普斯研究所 | 样品制备方法 |
| CN104350152B (zh) | 2012-06-01 | 2017-08-11 | 欧米伽生物技术公司 | 选择性核酸片段回收 |
| WO2014004726A1 (en) | 2012-06-26 | 2014-01-03 | Caifu Chen | Methods, compositions and kits for the diagnosis, prognosis and monitoring of cancer |
| EP2875149B1 (en) | 2012-07-20 | 2019-12-04 | Verinata Health, Inc. | Detecting and classifying copy number variation in a cancer genome |
| CN104685064A (zh) | 2012-07-24 | 2015-06-03 | 纳特拉公司 | 高度复合pcr方法和组合物 |
| US20140051585A1 (en) | 2012-08-15 | 2014-02-20 | Natera, Inc. | Methods and compositions for reducing genetic library contamination |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| ES2673315T3 (es) | 2012-08-28 | 2018-06-21 | Akonni Biosystems, Inc. | Método y kit para purificar ácidos nucleicos |
| EP4424826A3 (en) | 2012-09-04 | 2024-11-27 | Guardant Health, Inc. | Systems and methods to detect rare mutations and copy number variation |
| US20140065621A1 (en) | 2012-09-04 | 2014-03-06 | Natera, Inc. | Methods for increasing fetal fraction in maternal blood |
| EP2943590A4 (en) | 2013-01-13 | 2017-02-15 | Unitaq Bio | Methods and compositions for pcr using blocked and universal primers |
| WO2014122288A1 (en) | 2013-02-08 | 2014-08-14 | Qiagen Gmbh | Method for separating dna by size |
| US9982255B2 (en) | 2013-03-11 | 2018-05-29 | Kailos Genetics, Inc. | Capture methodologies for circulating cell free DNA |
| US10385394B2 (en) | 2013-03-15 | 2019-08-20 | The Translational Genomics Research Institute | Processes of identifying and characterizing X-linked disorders |
| ES3042844T3 (en) | 2013-03-15 | 2025-11-24 | Univ Leland Stanford Junior | Identification and use of circulating nucleic acid tumor markers |
| HK1222684A1 (zh) | 2013-03-15 | 2017-07-07 | 夸登特健康公司 | 检测稀有突变和拷贝数变异的系统和方法 |
| US20140272956A1 (en) | 2013-03-15 | 2014-09-18 | Abbott Molecular Inc. | Method for amplification and assay of rna fusion gene variants, method of distinguishing same and related primers, probes, and kits |
| US9499870B2 (en) | 2013-09-27 | 2016-11-22 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| WO2015083004A1 (en) | 2013-12-02 | 2015-06-11 | Population Genetics Technologies Ltd. | Method for evaluating minority variants in a sample |
| SG11201604923XA (en) | 2013-12-28 | 2016-07-28 | Guardant Health Inc | Methods and systems for detecting genetic variants |
| CN106029962A (zh) | 2014-02-11 | 2016-10-12 | 豪夫迈·罗氏有限公司 | 靶向测序和uid过滤 |
| DK3117012T3 (da) | 2014-03-14 | 2019-05-13 | Caredx Inc | Fremgangsmåder til overvågning af immunosuppressive terapier hos en transplantmodtager |
| CN106462669A (zh) | 2014-03-25 | 2017-02-22 | 奎斯特诊断投资股份有限公司 | 通过使用平均循环阈值的基因内差异表达(ide)检测基因融合 |
| JP6659575B2 (ja) | 2014-04-21 | 2020-03-04 | ナテラ, インコーポレイテッド | 変異の検出および染色体分節の倍数性 |
| WO2015164432A1 (en) | 2014-04-21 | 2015-10-29 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| US20180173846A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| ES2810300T3 (es) | 2014-07-03 | 2021-03-08 | Rhodx Inc | Etiquetado y evaluación de una secuencia diana |
| EP3169780B1 (en) | 2014-07-17 | 2020-02-12 | Qiagen GmbH | Method for isolating rna with high yield |
| EP3940703A1 (en) | 2014-07-18 | 2022-01-19 | Illumina, Inc. | Non-invasive prenatal diagnosis of fetal genetic conditions using cellular dna and cell free dna |
| CA2965500A1 (en) | 2014-10-24 | 2016-04-28 | Abbott Molecular Inc. | Enrichment of small nucleic acids |
| DK3218519T3 (da) | 2014-11-11 | 2020-12-21 | Bgi Shenzhen | Multipass-sekvensering |
| WO2016138080A1 (en) | 2015-02-24 | 2016-09-01 | Trustees Of Boston University | Protection of barcodes during dna amplification using molecular hairpins |
| WO2016138500A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Methods and compositions for barcoding nucleic acids for sequencing |
| EP3277843A2 (en) | 2015-03-30 | 2018-02-07 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| KR101850437B1 (ko) | 2015-04-14 | 2018-04-20 | 이원다이애그노믹스(주) | 차세대 염기서열 분석기법을 이용한 장기 이식 거부 반응 예측 방법 |
| WO2016172373A1 (en) | 2015-04-23 | 2016-10-27 | Cellular Research, Inc. | Methods and compositions for whole transcriptome amplification |
| US10844428B2 (en) | 2015-04-28 | 2020-11-24 | Illumina, Inc. | Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS) |
| DK3294906T3 (en) | 2015-05-11 | 2024-08-05 | Natera Inc | Methods for determining ploidy |
| WO2016193490A1 (en) | 2015-06-05 | 2016-12-08 | Qiagen Gmbh | Method for separating dna by size |
| US20180282796A1 (en) | 2015-09-29 | 2018-10-04 | Ludwig Institute For Cancer Research Ltd | Typing and Assembling Discontinuous Genomic Elements |
| ES3002715T3 (en) | 2016-04-14 | 2025-03-07 | Guardant Health Inc | Methods for early detection of cancer |
| CN109477138A (zh) | 2016-04-15 | 2019-03-15 | 纳特拉公司 | 肺癌检测方法 |
| US12043856B2 (en) | 2016-05-24 | 2024-07-23 | The Translational Genomics Research Institute | Molecular tagging methods and sequencing libraries |
| EP3464626B1 (en) | 2016-05-27 | 2022-04-06 | Sequenom, Inc. | Methods for detecting genetic variations |
| EP3510171A4 (en) | 2016-07-01 | 2020-04-29 | Natera, Inc. | COMPOSITIONS AND METHODS FOR DETECTION OF NUCLEIC ACID MUTATIONS |
| WO2018067517A1 (en) | 2016-10-04 | 2018-04-12 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| US11608497B2 (en) | 2016-11-08 | 2023-03-21 | Becton, Dickinson And Company | Methods for cell label classification |
| US10011870B2 (en) | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
| US10894976B2 (en) | 2017-02-21 | 2021-01-19 | Natera, Inc. | Compositions, methods, and kits for isolating nucleic acids |
| US11091800B2 (en) | 2017-09-20 | 2021-08-17 | University Of Utah Research Foundation | Size-selection of cell-free DNA for increasing family size during next-generation sequencing |
| EP4245863A3 (en) | 2018-01-12 | 2024-01-24 | Natera, Inc. | Novel primers and uses thereof |
| WO2019161244A1 (en) | 2018-02-15 | 2019-08-22 | Natera, Inc. | Methods for isolating nucleic acids with size selection |
| CN112236535A (zh) | 2018-04-14 | 2021-01-15 | 纳特拉公司 | 用于借助于循环肿瘤dna的个人化检测的癌症检测和监测的方法 |
| EP3807884A1 (en) | 2018-06-12 | 2021-04-21 | Natera, Inc. | Methods and systems for calling mutations |
| US20230287497A1 (en) | 2018-07-03 | 2023-09-14 | Natera, Inc. | Methods for detection of donor-derived cell-free dna |
| CN112639982A (zh) | 2018-07-17 | 2021-04-09 | 纳特拉公司 | 使用神经网络调用倍性状态的方法和系统 |
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Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 22/12/2011, OBSERVADAS AS CONDICOES LEGAIS. |