HRP20190209T4 - Detekcija i kvantifikacija acelularne dnk darivatelja u krvotoku primatelja presađivanja organa - Google Patents
Detekcija i kvantifikacija acelularne dnk darivatelja u krvotoku primatelja presađivanja organa Download PDFInfo
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- HRP20190209T4 HRP20190209T4 HRP20190209TT HRP20190209T HRP20190209T4 HR P20190209 T4 HRP20190209 T4 HR P20190209T4 HR P20190209T T HRP20190209T T HR P20190209TT HR P20190209 T HRP20190209 T HR P20190209T HR P20190209 T4 HRP20190209 T4 HR P20190209T4
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- 238000011002 quantification Methods 0.000 title claims 4
- 238000001514 detection method Methods 0.000 title 1
- 210000000056 organ Anatomy 0.000 title 1
- 238000000034 method Methods 0.000 claims 22
- 108700028369 Alleles Proteins 0.000 claims 20
- 210000002216 heart Anatomy 0.000 claims 3
- 210000003734 kidney Anatomy 0.000 claims 3
- 210000004185 liver Anatomy 0.000 claims 3
- 210000004369 blood Anatomy 0.000 claims 2
- 239000008280 blood Substances 0.000 claims 2
- 210000000265 leukocyte Anatomy 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- 238000012544 monitoring process Methods 0.000 claims 2
- 210000005259 peripheral blood Anatomy 0.000 claims 2
- 239000011886 peripheral blood Substances 0.000 claims 2
- 238000003753 real-time PCR Methods 0.000 claims 2
- 210000002966 serum Anatomy 0.000 claims 1
- 238000002054 transplantation Methods 0.000 claims 1
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/172—Haplotypes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/24—Immunology or allergic disorders
- G01N2800/245—Transplantation related diseases, e.g. graft versus host disease
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Transplanting Machines (AREA)
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Claims (15)
1. Metoda za procjenu integriteta transplantata kod primatelja, pri čemu se metoda sastoji od praćenja razine acelularne DNK presatka (cfDNK) procjenjivanjem količine SNP alela darivatelja u uzorku cfDNK dobivenom iz krvi primatelja, naznačena time što je SNP alel prethodno odabran kao alel s manjom frekvencijom alela (MAF) od najmanje 0.4, i dodatno, naznačena time, gdjr je SNP alela darivatelja prisutan u darivatelju, a primatelj je homozigotan za alternativni alel za taj SNP, gdje je SNP alel identificiran bez korištenja zasebnog uzorka od darivatelja.
2. Metoda prema patentnom zahtjevu 1, naznačena time, što se kvantifikacija razine SNP alela darivatelja u uzorku cfDNK sastoji od utvrđivanja postotka SNP alela darivatelja u uzorku cfDNK.
3. Metoda prema patentnom zahtjevu 1 ili 2, naznačena time, što se uzorak cfDNK procjenjuje tijekom dana, tjedana, mjeseci ili godina.
4. Metoda prema bilo kojem patentnom zahtjevu 1-3, naznačena time, gdje je darivateljsko tkivo je jetra, srce ili bubreg.
5. Metoda za detekciju SNP-a od darivatelja radi praćenja integriteta transplantata kod primatelja koji prima tkivo od predmetnog darivatelja, pri čemu se metoda sastoji od:
(a) utvrđivanja da je SNP koji ima manju frekvenciju alela od 0.4 ili više homozigotan kod primatelja;
(b) pojačavanja acelularne (cfDNK) iz uzorka seruma ili plazme dobivenog od primatelja najmanje 5 dana nakon presađivanja materijala od darivatelja da bi se stvorila cf biblioteka;
(c) obavljanja reakcije kvantitativnog PCR-a na SNP-ovima utvrđenih u (a) radi detekcije prisutnosti alternativnog alela za jedan ili više SNP-ova; i
(d) odabira SNP-a u kojima je alternativni SNP prisutan u cf biblioteci radi praćenja integriteta transplantata kod pacijenta;
pri čemu metoda ne koristi poseban uzorak od darivatelja.
6. Metoda prema patentnom zahtjevu 5, naznačena time, što se korak (a) obavlja pomoću DNK izolirane iz leukocita periferne krvi dobivenih od primatelja ili pomoću cfDNK iz koraka (b).
7. Metoda prema patentnom zahtjevu 5 ili 6, naznačena time, što SNP koji ima manju frekvenciju alela od 0.4 ili više je SNP naveden u tablici 1.
8. Metoda prema bilo kojem od patentnih zahtjeva 5-7, naznačena time, što SNP odabran u koraku (d) je homozigotan kod darivatelja.
9. Metoda za detekciju SNP-a od darivatelja radi praćenja integriteta transplantata kod primatelja koji prima tkivo od predmetnog darivatelja, pri čemu se metoda sastoji od:
(a) utvrđivanja da je SNP koji ima manju frekvenciju alela od 0.4 ili više homozigotan kod primatelja;
(b) pojačavanja acelularne (cfDNK) iz uzorka krvi dobivenog od primatelja 24 sata ili manje nakon presađivanja materijala presatka od darivatelja da bi se stvorila cf biblioteka;
(c) utvrđivanja da je SNP koji ima manju frekvenciju alela od 0.4 ili više homozigotan kod darivatelja pomoću cf biblioteke iz koraka (b);
(d) obavljanja reakcije kvantitativnog PCR-a na SNP-ovima utvrđenih u (a) radi detekcije prisutnosti alternativnog alela za jedan ili više SNP-ova u darivatelja, i
(e) odabira SNP-a u kojem je alternativni SNP homozigotan kod darivatelja;
pri čemu metoda ne koristi poseban uzorak od darivatelja.
10. Metoda prema patentnom zahtjevu 9, naznačena time, što se korak (a) obavlja pomoću DNK izolirane iz leukocita periferne krvi dobivenih od pacijenta.
11. Metoda prema patentnom zahtjevu 9 ili 10, naznačena time, što SNP koji ima manju frekvenciju alela od 0.4 ili više je SNP naveden u tablici 1.
12. Metoda prema bilo kojem patentnom zahtjevu 5 do 11, koja se dodatno sastoji od uzimanja uzorka cfDNK od primatelja nakon presađivanja tkiva darivatelja; i
kvantifikacije razine SNP alela darivatelja u uzorku cfDNK, opcionalno naznačena time, što
(i) korak kvantifikacije se sastoji od utvrđivanja postotka SNP alela darivatelja u uzorku cfDNK; i/ili
(ii) darivateljsko tkivo je jetra, srce ili bubreg.
13. Metoda za praćenje integriteta transplatata kod primatelja transplantata, pri čemu se metoda sastoji od kvantifikacije, u uzorku cfDNK uzetom od primatelja transplanta, količine SNP alela darivatelja za SNP odabran prema u bilo kojem patentnom zahtjevu od 1 do 9; pri čemu se alel SNP identificira bez upotrebe posebnog uzorka od darivatelja.
14. Metoda prema patentnom zahtjevu 13, pri čemu je transplantant jetra, bubreg ili srce.
15. Metoda prema bilo kojem patentnom zahtjevu od 1 do 4, naznačena time, što je SNP odabran pomoću metode iz bilo kojeg patentnog zahtjeva od 5 do 12.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361828553P | 2013-05-29 | 2013-05-29 | |
EP14804474.6A EP3004388B2 (en) | 2013-05-29 | 2014-05-29 | Detection and quantification of donor cell-free dna in the circulation of organ transplant recipients |
PCT/US2014/040055 WO2014194113A2 (en) | 2013-05-29 | 2014-05-29 | Detection and quantification of donor cell-free dna in the circulation of organ transplant recipients |
Publications (2)
Publication Number | Publication Date |
---|---|
HRP20190209T1 HRP20190209T1 (hr) | 2019-04-05 |
HRP20190209T4 true HRP20190209T4 (hr) | 2023-09-15 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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HRP20190209TT HRP20190209T4 (hr) | 2013-05-29 | 2014-05-29 | Detekcija i kvantifikacija acelularne dnk darivatelja u krvotoku primatelja presađivanja organa |
Country Status (12)
Country | Link |
---|---|
US (3) | US20160115541A1 (hr) |
EP (1) | EP3004388B2 (hr) |
CY (1) | CY1122332T1 (hr) |
DK (1) | DK3004388T4 (hr) |
ES (1) | ES2704682T5 (hr) |
HR (1) | HRP20190209T4 (hr) |
HU (1) | HUE041935T2 (hr) |
LT (1) | LT3004388T (hr) |
PL (1) | PL3004388T5 (hr) |
PT (1) | PT3004388T (hr) |
SI (1) | SI3004388T2 (hr) |
WO (1) | WO2014194113A2 (hr) |
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PL3117012T3 (pl) * | 2014-03-14 | 2019-08-30 | Caredx, Inc. | Sposoby monitorowania terapii immunosupresyjnych u biorcy przeszczepu |
-
2014
- 2014-05-29 LT LTEP14804474.6T patent/LT3004388T/lt unknown
- 2014-05-29 DK DK14804474.6T patent/DK3004388T4/da active
- 2014-05-29 HR HRP20190209TT patent/HRP20190209T4/hr unknown
- 2014-05-29 PT PT14804474T patent/PT3004388T/pt unknown
- 2014-05-29 WO PCT/US2014/040055 patent/WO2014194113A2/en active Application Filing
- 2014-05-29 ES ES14804474T patent/ES2704682T5/es active Active
- 2014-05-29 HU HUE14804474A patent/HUE041935T2/hu unknown
- 2014-05-29 US US14/893,807 patent/US20160115541A1/en not_active Abandoned
- 2014-05-29 PL PL14804474.6T patent/PL3004388T5/pl unknown
- 2014-05-29 SI SI201431041T patent/SI3004388T2/sl unknown
- 2014-05-29 EP EP14804474.6A patent/EP3004388B2/en active Active
-
2018
- 2018-03-13 US US15/920,356 patent/US11155872B2/en active Active
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2019
- 2019-01-24 CY CY20191100106T patent/CY1122332T1/el unknown
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2021
- 2021-10-15 US US17/503,056 patent/US20220025459A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US11155872B2 (en) | 2021-10-26 |
EP3004388A4 (en) | 2017-01-11 |
WO2014194113A3 (en) | 2015-01-22 |
CY1122332T1 (el) | 2021-01-27 |
HRP20190209T1 (hr) | 2019-04-05 |
EP3004388B1 (en) | 2018-10-31 |
ES2704682T5 (es) | 2023-11-10 |
PT3004388T (pt) | 2019-01-29 |
SI3004388T2 (sl) | 2023-11-30 |
SI3004388T1 (sl) | 2019-03-29 |
LT3004388T (lt) | 2019-01-25 |
WO2014194113A2 (en) | 2014-12-04 |
US20180346982A1 (en) | 2018-12-06 |
US20220025459A1 (en) | 2022-01-27 |
EP3004388A2 (en) | 2016-04-13 |
US20160115541A1 (en) | 2016-04-28 |
HUE041935T2 (hu) | 2019-06-28 |
EP3004388B2 (en) | 2023-05-31 |
DK3004388T3 (en) | 2019-02-04 |
PL3004388T5 (pl) | 2023-10-09 |
PL3004388T3 (pl) | 2019-04-30 |
DK3004388T4 (da) | 2023-08-28 |
ES2704682T3 (es) | 2019-03-19 |
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