MX2022010038A - Metodos y materiales para la evaluacion de acidos nucleicos. - Google Patents
Metodos y materiales para la evaluacion de acidos nucleicos.Info
- Publication number
- MX2022010038A MX2022010038A MX2022010038A MX2022010038A MX2022010038A MX 2022010038 A MX2022010038 A MX 2022010038A MX 2022010038 A MX2022010038 A MX 2022010038A MX 2022010038 A MX2022010038 A MX 2022010038A MX 2022010038 A MX2022010038 A MX 2022010038A
- Authority
- MX
- Mexico
- Prior art keywords
- methods
- materials
- nucleic acids
- assessing nucleic
- systems
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/531—Glycosylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
En la presente se proporcionan sistemas, kits, composiciones y métodos para la preparación de bibliotecas de secuenciación y el flujo de trabajo de secuenciación (por ejemplo, para la identificación de mutaciones). En ciertas modalidades, se proporcionan en la presente sistemas y métodos para codificar en barras de forma idéntica ambas cadenas de plantillas, y el enriquecimiento a base de PCR de cada cadena que no requiere captura por hibridación.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202062977066P | 2020-02-14 | 2020-02-14 | |
PCT/US2021/017937 WO2021163546A1 (en) | 2020-02-14 | 2021-02-12 | Methods and materials for assessing nucleic acids |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2022010038A true MX2022010038A (es) | 2022-09-05 |
Family
ID=77292957
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2022010038A MX2022010038A (es) | 2020-02-14 | 2021-02-12 | Metodos y materiales para la evaluacion de acidos nucleicos. |
Country Status (11)
Country | Link |
---|---|
US (1) | US20220073977A1 (es) |
EP (1) | EP4103748A4 (es) |
JP (1) | JP2023513606A (es) |
KR (1) | KR20220154690A (es) |
CN (1) | CN115516108A (es) |
AU (1) | AU2021219794A1 (es) |
BR (1) | BR112022015909A2 (es) |
CA (1) | CA3170345A1 (es) |
IL (1) | IL295297A (es) |
MX (1) | MX2022010038A (es) |
WO (1) | WO2021163546A1 (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2023150277A1 (en) * | 2022-02-03 | 2023-08-10 | The Johns Hopkins University | Methods for sequencing an immune cell receptor |
WO2024073034A1 (en) * | 2022-09-29 | 2024-04-04 | The Board Of Trustees Of The Leland Stanford Junior University | Simplified sequencing library preparation for dna |
WO2024091545A1 (en) * | 2022-10-25 | 2024-05-02 | Cornell University | Nucleic acid error suppression |
CN115928225A (zh) * | 2023-01-03 | 2023-04-07 | 京东方科技集团股份有限公司 | 游离dna甲基化文库构建方法及其文库、应用和试剂盒 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3114231B1 (en) * | 2014-03-03 | 2019-01-02 | Swift Biosciences, Inc. | Enhanced adaptor ligation |
WO2016181128A1 (en) * | 2015-05-11 | 2016-11-17 | Genefirst Ltd | Methods, compositions, and kits for preparing sequencing library |
DK3573646T3 (da) * | 2017-01-27 | 2022-03-14 | Integrated Dna Tech Inc | Fremstilling af next-generation-sekventering (NGS)-biblioteker under anvendelse af kompetitiv strengfortrængning |
AU2018240559A1 (en) * | 2017-03-23 | 2019-09-19 | University Of Washington | Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing |
-
2021
- 2021-02-12 EP EP21753424.7A patent/EP4103748A4/en active Pending
- 2021-02-12 IL IL295297A patent/IL295297A/en unknown
- 2021-02-12 AU AU2021219794A patent/AU2021219794A1/en active Pending
- 2021-02-12 US US17/174,838 patent/US20220073977A1/en active Pending
- 2021-02-12 KR KR1020227031690A patent/KR20220154690A/ko unknown
- 2021-02-12 WO PCT/US2021/017937 patent/WO2021163546A1/en active Application Filing
- 2021-02-12 MX MX2022010038A patent/MX2022010038A/es unknown
- 2021-02-12 CN CN202180028441.0A patent/CN115516108A/zh active Pending
- 2021-02-12 JP JP2022549104A patent/JP2023513606A/ja active Pending
- 2021-02-12 BR BR112022015909A patent/BR112022015909A2/pt unknown
- 2021-02-12 CA CA3170345A patent/CA3170345A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
KR20220154690A (ko) | 2022-11-22 |
CA3170345A1 (en) | 2021-08-19 |
US20220073977A1 (en) | 2022-03-10 |
EP4103748A4 (en) | 2024-03-13 |
WO2021163546A1 (en) | 2021-08-19 |
AU2021219794A1 (en) | 2022-09-08 |
IL295297A (en) | 2022-10-01 |
BR112022015909A2 (pt) | 2022-10-04 |
EP4103748A1 (en) | 2022-12-21 |
CN115516108A (zh) | 2022-12-23 |
JP2023513606A (ja) | 2023-03-31 |
WO2021163546A9 (en) | 2023-09-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
MX2022010038A (es) | Metodos y materiales para la evaluacion de acidos nucleicos. | |
Tu et al. | TCR sequencing paired with massively parallel 3′ RNA-seq reveals clonotypic T cell signatures | |
Evrony et al. | Single-neuron sequencing analysis of L1 retrotransposition and somatic mutation in the human brain | |
WO2013023176A3 (en) | Polymerase compositions, methods of making and using same | |
WO2017201102A8 (en) | Method of improved sequencing by strand identification | |
Liu et al. | Systematic comparative evaluation of methods for investigating the TCRβ repertoire | |
FI3896171T3 (fi) | Menetelmä polynukleotidien prosessoimiseksi yksittäisten solujen analysointia varten | |
BRPI0814670B8 (pt) | método para realizar diagnóstico pré-natal de uma aneuploidia cromossômica fetal | |
CN105714383A (zh) | 一种基于分子反向探针的测序文库构建方法和试剂 | |
Gullo et al. | The transcriptomic landscape of gastric cancer: insights into Epstein-Barr virus infected and microsatellite unstable tumors | |
JP2019517774A (ja) | 核酸を濃縮及びキャプチャーするためのプローブと設計方法 | |
Koning et al. | ARTISAN PCR: rapid identification of full-length immunoglobulin rearrangements without primer binding bias | |
CN105524981A (zh) | 目标基因的捕获试剂盒及捕获方法 | |
CN104963000A (zh) | 一种快速构建单细胞dna测序文库的方法和试剂盒 | |
Kim et al. | Chromosome-scale assembly comparison of the Korean Reference Genome KOREF from PromethION and PacBio with Hi-C mapping information | |
Kolter et al. | Internal transcribed spacer primer evaluation for vascular plant metabarcoding | |
Niklas et al. | Qualifying high-throughput immune repertoire sequencing | |
Acha et al. | Analysis of Intratumoral heterogeneity in myelodysplastic syndromes with isolated del (5q) using a single cell approach | |
Xin et al. | Mitochondrial genome sequencing with short overlapping amplicons on MiSeq FGx system | |
Gouveia et al. | Massively parallel sequencing of forensic samples using precision ID mtDNA whole genome panel on the ion S5™ system | |
Mark et al. | A hierarchy of selection pressures determines the organization of the T cell receptor repertoire | |
Santosa et al. | 3D Chromatin dynamics during innate and adaptive immune memory acquisition | |
Speranskaya et al. | SCV-2000bp: a primer panel for SARS-CoV-2 full-genome sequencing | |
Bar et al. | A local sequence signature defines a subset of heterochromatin-associated CpGs with minimal loss of methylation in healthy tissues but extensive loss in cancer | |
Mogensen et al. | Template preparation of AmpliSeq™ libraries using the Ion Chef™ |