JP4511025B2 - 複数置換プロテアーゼ変異体 - Google Patents

複数置換プロテアーゼ変異体 Download PDF

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Publication number
JP4511025B2
JP4511025B2 JP2000517090A JP2000517090A JP4511025B2 JP 4511025 B2 JP4511025 B2 JP 4511025B2 JP 2000517090 A JP2000517090 A JP 2000517090A JP 2000517090 A JP2000517090 A JP 2000517090A JP 4511025 B2 JP4511025 B2 JP 4511025B2
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Japan
Prior art keywords
protease
amino acid
subtilisin
residue
acid substitution
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Expired - Fee Related
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JP2000517090A
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English (en)
Japanese (ja)
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JP2001520045A (ja
JP2001520045A5 (enExample
Inventor
シェレンバーガー，ヴォルカー
ティー ジュニア ケリス，ジェイムズ
ピーチ，クリスチャン
ナデルニー，ジョアンヌ
ピー ネイキ，ドナルド
ジェイ ポーロス，アイルーカーラン
ディー コリアー，キャセリン
エム コールドウェル，ロバート
セー ベーク，アンドレ
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Procter and Gamble Co
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Procter and Gamble Co
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Application filed by Procter and Gamble Co filed Critical Procter and Gamble Co
Publication of JP2001520045A publication Critical patent/JP2001520045A/ja
Publication of JP2001520045A5 publication Critical patent/JP2001520045A5/ja
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Publication of JP4511025B2 publication Critical patent/JP4511025B2/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681—Chemically modified or immobilised enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G4/00—Chewing gum
    • A23G4/06—Chewing gum characterised by the composition containing organic or inorganic compounds
    • A23G4/12—Chewing gum characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
    • A23G4/123—Chewing gum characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins containing microorganisms, enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/39—Organic or inorganic per-compounds
    • C11D3/3902—Organic or inorganic per-compounds combined with specific additives
    • C11D3/3905—Bleach activators or bleach catalysts
    • C11D3/3907—Organic compounds
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/39—Organic or inorganic per-compounds
    • C11D3/3945—Organic per-compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/86—Products or compounds obtained by genetic engineering
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/12—Soft surfaces, e.g. textile
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/14—Hard surfaces

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Polymers & Plastics (AREA)
  • Inorganic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Dermatology (AREA)
  • Molecular Biology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Animal Husbandry (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Cosmetics (AREA)
  • Confectionery (AREA)
  • Exhaust Gas After Treatment (AREA)
  • Slot Machines And Peripheral Devices (AREA)
JP2000517090A 1997-10-23 1998-10-23 複数置換プロテアーゼ変異体 Expired - Fee Related JP4511025B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US95632497A 1997-10-23 1997-10-23
US95656497A 1997-10-23 1997-10-23
US95632397A 1997-10-23 1997-10-23
US08/956,324 1997-10-23
US08/956,323 1997-10-23
US08/956,564 1997-10-23
PCT/US1998/022572 WO1999020770A2 (en) 1997-10-23 1998-10-23 Multiply-substituted protease variants

Publications (3)

Publication Number Publication Date
JP2001520045A JP2001520045A (ja) 2001-10-30
JP2001520045A5 JP2001520045A5 (enExample) 2006-01-05
JP4511025B2 true JP4511025B2 (ja) 2010-07-28

Family

ID=27420734

Family Applications (7)

Application Number Title Priority Date Filing Date
JP2000517090A Expired - Fee Related JP4511025B2 (ja) 1997-10-23 1998-10-23 複数置換プロテアーゼ変異体
JP2000517046A Pending JP2001520305A (ja) 1997-10-23 1998-10-23 多置換プロテアーゼ変種およびアミラーゼ変種を含有したクリーニング組成物
JP2000517091A Pending JP2001520046A (ja) 1997-10-23 1998-10-23 洗剤で使用される改変された正味の特性を有する複数置換プロテアーゼ変異体
JP2000517050A Pending JP2001520308A (ja) 1997-10-23 1998-10-23 多置換プロテアーゼ変種を含有したクリーニング組成物
JP2000517049A Pending JP2001520307A (ja) 1997-10-23 1998-10-23 多置換プロテアーゼ変種を含有した漂白組成物
JP2000517089A Expired - Fee Related JP4346237B2 (ja) 1997-10-23 1998-10-23 複数置換プロテアーゼ変異体
JP2012138496A Expired - Fee Related JP5612026B2 (ja) 1997-10-23 2012-06-20 洗剤で使用される改変された正味の特性を有する複数置換プロテアーゼ変異体

Family Applications After (6)

Application Number Title Priority Date Filing Date
JP2000517046A Pending JP2001520305A (ja) 1997-10-23 1998-10-23 多置換プロテアーゼ変種およびアミラーゼ変種を含有したクリーニング組成物
JP2000517091A Pending JP2001520046A (ja) 1997-10-23 1998-10-23 洗剤で使用される改変された正味の特性を有する複数置換プロテアーゼ変異体
JP2000517050A Pending JP2001520308A (ja) 1997-10-23 1998-10-23 多置換プロテアーゼ変種を含有したクリーニング組成物
JP2000517049A Pending JP2001520307A (ja) 1997-10-23 1998-10-23 多置換プロテアーゼ変種を含有した漂白組成物
JP2000517089A Expired - Fee Related JP4346237B2 (ja) 1997-10-23 1998-10-23 複数置換プロテアーゼ変異体
JP2012138496A Expired - Fee Related JP5612026B2 (ja) 1997-10-23 2012-06-20 洗剤で使用される改変された正味の特性を有する複数置換プロテアーゼ変異体

Country Status (26)

Country Link
US (5) US6312936B1 (enExample)
EP (10) EP1025240B1 (enExample)
JP (7) JP4511025B2 (enExample)
KR (6) KR20010024554A (enExample)
CN (9) CN1198912C (enExample)
AR (10) AR016969A1 (enExample)
AT (9) ATE513479T1 (enExample)
AU (7) AU742632B2 (enExample)
BR (6) BRPI9813115B1 (enExample)
CA (9) CA2672492A1 (enExample)
CZ (6) CZ20001478A3 (enExample)
DE (7) DE69833015T2 (enExample)
DK (9) DK1624050T3 (enExample)
EG (2) EG21711A (enExample)
ES (7) ES2255189T3 (enExample)
HK (1) HK1039159B (enExample)
HU (3) HUP0104539A3 (enExample)
ID (4) ID25897A (enExample)
MA (2) MA25044A1 (enExample)
NO (3) NO326992B1 (enExample)
NZ (3) NZ520770A (enExample)
PL (1) PL191529B1 (enExample)
PT (8) PT1025239E (enExample)
TR (8) TR200101861T2 (enExample)
TW (2) TW585912B (enExample)
WO (5) WO1999020770A2 (enExample)

Families Citing this family (620)

* Cited by examiner, † Cited by third party
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MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
ATE385254T1 (de) * 1997-08-29 2008-02-15 Novozymes As Proteasevarianten und zusammensetzungen
MA25044A1 (fr) * 1997-10-23 2000-10-01 Procter & Gamble Compositions de lavage contenant des variants de proteases multisubstituees.
ES2368718T3 (es) * 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
DE69939538D1 (de) 1998-07-02 2008-10-23 Univ Toronto Mit einer kohlenhydratgruppe chemisch modifizierte proteine
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JP4932990B2 (ja) 1998-11-10 2012-05-16 ダニスコ・ユーエス・インコーポレイテッド 化学修飾突然変異体セリンヒドロラーゼは改良された触媒活性およびキラル選択性を示す
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JP2003516116A (ja) * 1999-07-22 2003-05-13 ザ、プロクター、エンド、ギャンブル、カンパニー 立体的に保護されたクリップ部位を有するプロテアーゼ抱合体
KR20020021398A (ko) * 1999-07-22 2002-03-20 데이비드 엠 모이어 정의된 에피토프 구역에 아미노산 치환을 가진 서브틸리신프로테아제 변이체
MXPA02002682A (es) * 1999-09-09 2002-07-30 Procter & Gamble Una composicion de detergente que contiene una proteasa.
KR100387166B1 (ko) * 1999-12-15 2003-06-12 닛뽄덴끼 가부시끼가이샤 유기 일렉트로 루미네선스 소자
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US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6902922B2 (en) * 2000-04-03 2005-06-07 Novozymes A/S Subtilisin variants
AU2001254623A1 (en) * 2000-04-28 2001-11-12 Novozymes A/S Production and use of protein variants having modified immunogenicity
AU2000260631A1 (en) * 2000-06-30 2002-01-14 The Procter & Gamble Company Detergent compositions comprising a maltogenic alpha-amylase enzyme
BR0017277A (pt) * 2000-06-30 2003-05-06 Procter & Gamble Composições detergentes compreendendo uma enzima ciclodextrina glicanotransferase
MXPA03000793A (es) 2000-07-28 2003-06-04 Henkel Kgaa Enzima amilolitica novedosa, extraida de bacilo sp a 7-7 (dsm 12368) y agentes de lavado y limpiadores que contienen esta enzima amilolitica novedosa.
US20020155574A1 (en) 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
US6893855B2 (en) * 2000-10-13 2005-05-17 Novozymes A/S Subtilase variants
US6803222B2 (en) 2000-11-22 2004-10-12 Kao Corporation Alkaline proteases
US7303907B2 (en) 2001-01-08 2007-12-04 Health Protection Agency Degradation and detection of TSE infectivity
EP1241112A3 (en) 2001-03-15 2003-02-26 The Procter & Gamble Company Flexible multiple compartment pouch
EP1373296B1 (en) * 2001-03-23 2011-10-05 Genencor International, Inc. Proteins producing an altered immunogenic response and methods of making and using the same
WO2002092797A2 (en) 2001-05-15 2002-11-21 Novozymes A/S Alpha-amylase variant with altered properties
DK200101090A (da) 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
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DE60330346D1 (de) * 2002-01-16 2010-01-14 Genencor Int Mehrfachsubstituierte proteasevarianten
BR0306956A (pt) * 2002-01-16 2006-04-11 Genencor Int variantes de protease multiplamente substituìdas
US20030180281A1 (en) * 2002-03-11 2003-09-25 Bott Richard R. Preparations for topical skin use and treatment
US7223386B2 (en) * 2002-03-11 2007-05-29 Dow Corning Corporation Preparations for topical skin use and treatment
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CN100399930C (zh) 2002-07-29 2008-07-09 安敏诺技术公司 肽/氨基酸的生产方法、通过所述方法生产的肽/氨基酸及其应用
US7082689B2 (en) * 2002-10-11 2006-08-01 Black & Decker Inc. Keyless shoe lock for reciprocating saw
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HUP0104539A3 (en) 2002-12-28

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