AR015977A1 - Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes - Google Patents

Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes

Info

Publication number
AR015977A1
AR015977A1 ARP980105279A ARP980105279A AR015977A1 AR 015977 A1 AR015977 A1 AR 015977A1 AR P980105279 A ARP980105279 A AR P980105279A AR P980105279 A ARP980105279 A AR P980105279A AR 015977 A1 AR015977 A1 AR 015977A1
Authority
AR
Argentina
Prior art keywords
protease
charge
precursor
amino acid
variant
Prior art date
Application number
ARP980105279A
Other languages
English (en)
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27420734&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR015977(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genencor Int filed Critical Genencor Int
Publication of AR015977A1 publication Critical patent/AR015977A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681—Chemically modified or immobilised enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G4/00—Chewing gum
    • A23G4/06—Chewing gum characterised by the composition containing organic or inorganic compounds
    • A23G4/12—Chewing gum characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
    • A23G4/123—Chewing gum characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins containing microorganisms, enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/39—Organic or inorganic per-compounds
    • C11D3/3902—Organic or inorganic per-compounds combined with specific additives
    • C11D3/3905—Bleach activators or bleach catalysts
    • C11D3/3907—Organic compounds
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/39—Organic or inorganic per-compounds
    • C11D3/3945—Organic per-compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/86—Products or compounds obtained by genetic engineering
    • C11D2111/12—
    • C11D2111/14—

Abstract

Se describen variantes de proteasa derivadas de secuencias de ADN de proteasasnaturales o recombinantes. Las proteasas variantes se obtienen en generalmediante la modificacion in vitro de una secuencia de ADN precursora que codifica la proteasanatur al o recombinante para generar la substitucion de unapluralidad de residuos aminoácidos en la secuencia de aminoácido de una proteasa precursora. Se proveen variantes de proteasa que contienen substituciones delos aminoácidos en una o másposiciones del residuo, de manera que la substitucion altera la carga en esta posicion para hacer que la carga sea más negativa omenos positiva en comparacion con una proteasa precursora, y por lo tanto que la variante sea más eficaz en un sistemade baja conce ntracion de detergente queuna proteasa precursora. Asimismo, se proveen variantes de proteasa que contienen substituciones de los aminoácidos en una o más posiciones del residuo demanera que la substitucion altera la carga en estaposicion para hacer que la carga sea más positiva o menos negativa en comparacion con una proteasaprecursora y por lo tanto la variante de proteasa es más eficaz en un sistema de alta concentracion de detergente que una proteasa precursora. Seproveenvariantes de prote asa que contienen substituciones de los aminoácidos en una o más posiciones de residuo, de manera que la substitucion altera la carga en estaposicion para hacer que la carga sea más negativa o menos positiva en comparacioncon una proteasa precursora , y por lo tanto la variante de proteasa es máseficaz en un sistema de concentracion de detergente intermedia que en una proteasa precursora. Asimismo, se proveen variantes de proteasa que contienensubstituciones de losaminoácidos en una o más posic iones del residuo, de manera que la substitucion altera la carga en esta posicion para hacer que la cargasea más positiva o menos negativa en comparacion con una proteasa precursora y por lo tanto la variante deproteasa es más eficaz en un sistema d econcentracion de detergente intermedia que una proteasa precursora. Además, se provee un método para producir una variante de proteasa que es más eficaz en un
ARP980105279A 1997-10-23 1998-10-22 Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes AR015977A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US95632497A 1997-10-23 1997-10-23
US95656497A 1997-10-23 1997-10-23
US95632397A 1997-10-23 1997-10-23

Publications (1)

Publication Number Publication Date
AR015977A1 true AR015977A1 (es) 2001-05-30

Family

ID=27420734

Family Applications (10)

Application Number Title Priority Date Filing Date
ARP980105280A AR016969A1 (es) 1997-10-23 1998-10-22 VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
ARP980105279A AR015977A1 (es) 1997-10-23 1998-10-22 Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
ARP980105303A AR013718A1 (es) 1997-10-23 1998-10-23 Composiciones blanqueadoras que contienen variantes de proteasa sustituidas en forma multiple.
ARP980105305A AR013719A1 (es) 1997-10-23 1998-10-23 Composiciones limpiadoras que contienen variantes de proteasa sustituida en forma multiple
ARP060100108A AR052353A2 (es) 1997-10-23 2006-01-11 Variante de proteasa, adn, vector de expresion, celula huesped, composicion de limpieza, alimento para animales y composicion para tratar textiles
ARP060100111A AR052354A2 (es) 1997-10-23 2006-01-11 Metodos para mejorar el desempeno de una subtilisina en sistemas de baja y alta concentracion de detergente, y metodo para producir una composicion detergente
ARP060100112A AR052355A2 (es) 1997-10-23 2006-01-11 Variante de proteasa, alimento para animales, adn, vector de expresion, celula huesped, composicion de limpieza, alimento para animales y composicion para tratar textiles
ARP060100107A AR052281A2 (es) 1997-10-23 2006-01-11 Composicion blanqueadora, metodo para la limpieza de telas, metodo para limpiar vajilla, metodo para la limpieza personal, metodo para pretratar una tela que necesita ser limpiada, composicion para limpieza, composicion para limpieza personal y metodo para pretratar una tela que necesita ser limpiad
ARP060100109A AR052282A2 (es) 1997-10-23 2006-01-11 Composicion para limpieza de telas y/o de vajilla y/o de superficies duras, metodo para la limpieza de telas, metodo para limpiar vajilla, composicion para limpieza personal, metodo para la limpieza personal, composicion para limpieza de telas y/o de vajilla y/o de superficies duras, composicion par
ARP060100110A AR052453A2 (es) 1997-10-23 2006-01-11 Composicion para limpieza de telas y/o de vajilla y/o de superficies duras, metodo para la limpieza de telas, metodo para limpiar vajilla, composicion para limpieza personal, metodo para la limpieza personal y metodo pretratar una tela que necesita ser limpiada

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP980105280A AR016969A1 (es) 1997-10-23 1998-10-22 VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL

Family Applications After (8)

Application Number Title Priority Date Filing Date
ARP980105303A AR013718A1 (es) 1997-10-23 1998-10-23 Composiciones blanqueadoras que contienen variantes de proteasa sustituidas en forma multiple.
ARP980105305A AR013719A1 (es) 1997-10-23 1998-10-23 Composiciones limpiadoras que contienen variantes de proteasa sustituida en forma multiple
ARP060100108A AR052353A2 (es) 1997-10-23 2006-01-11 Variante de proteasa, adn, vector de expresion, celula huesped, composicion de limpieza, alimento para animales y composicion para tratar textiles
ARP060100111A AR052354A2 (es) 1997-10-23 2006-01-11 Metodos para mejorar el desempeno de una subtilisina en sistemas de baja y alta concentracion de detergente, y metodo para producir una composicion detergente
ARP060100112A AR052355A2 (es) 1997-10-23 2006-01-11 Variante de proteasa, alimento para animales, adn, vector de expresion, celula huesped, composicion de limpieza, alimento para animales y composicion para tratar textiles
ARP060100107A AR052281A2 (es) 1997-10-23 2006-01-11 Composicion blanqueadora, metodo para la limpieza de telas, metodo para limpiar vajilla, metodo para la limpieza personal, metodo para pretratar una tela que necesita ser limpiada, composicion para limpieza, composicion para limpieza personal y metodo para pretratar una tela que necesita ser limpiad
ARP060100109A AR052282A2 (es) 1997-10-23 2006-01-11 Composicion para limpieza de telas y/o de vajilla y/o de superficies duras, metodo para la limpieza de telas, metodo para limpiar vajilla, composicion para limpieza personal, metodo para la limpieza personal, composicion para limpieza de telas y/o de vajilla y/o de superficies duras, composicion par
ARP060100110A AR052453A2 (es) 1997-10-23 2006-01-11 Composicion para limpieza de telas y/o de vajilla y/o de superficies duras, metodo para la limpieza de telas, metodo para limpiar vajilla, composicion para limpieza personal, metodo para la limpieza personal y metodo pretratar una tela que necesita ser limpiada

Country Status (26)

Country Link
US (5) US6312936B1 (es)
EP (10) EP1025240B1 (es)
JP (7) JP4346237B2 (es)
KR (6) KR20010031380A (es)
CN (9) CN100506963C (es)
AR (10) AR016969A1 (es)
AT (9) ATE399469T1 (es)
AU (7) AU1199699A (es)
BR (6) BRPI9813266B1 (es)
CA (9) CA2306894C (es)
CZ (6) CZ20001479A3 (es)
DE (7) DE69839057T2 (es)
DK (9) DK1027418T4 (es)
EG (2) EG22075A (es)
ES (7) ES2255189T3 (es)
HK (1) HK1039159B (es)
HU (3) HUP0104539A3 (es)
ID (4) ID28256A (es)
MA (2) MA25044A1 (es)
NO (3) NO327607B1 (es)
NZ (3) NZ520770A (es)
PL (1) PL191529B1 (es)
PT (8) PT1027418E (es)
TR (8) TR200001112T2 (es)
TW (2) TW585912B (es)
WO (5) WO1999020723A2 (es)

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