DE60330346D1 - Mehrfachsubstituierte proteasevarianten - Google Patents

Mehrfachsubstituierte proteasevarianten

Info

Publication number
DE60330346D1
DE60330346D1 DE60330346T DE60330346T DE60330346D1 DE 60330346 D1 DE60330346 D1 DE 60330346D1 DE 60330346 T DE60330346 T DE 60330346T DE 60330346 T DE60330346 T DE 60330346T DE 60330346 D1 DE60330346 D1 DE 60330346D1
Authority
DE
Germany
Prior art keywords
protease
amino acid
precursor
naturally
occurring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60330346T
Other languages
English (en)
Inventor
Richard R Bott
David A Estell
James Kellis Jr
Ayrookaran J Poulose
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Application granted granted Critical
Publication of DE60330346D1 publication Critical patent/DE60330346D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)
DE60330346T 2002-01-16 2003-01-16 Mehrfachsubstituierte proteasevarianten Expired - Lifetime DE60330346D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35022202P 2002-01-16 2002-01-16
PCT/US2003/001448 WO2003062381A2 (en) 2002-01-16 2003-01-16 Multiply-substituted protease variants

Publications (1)

Publication Number Publication Date
DE60330346D1 true DE60330346D1 (de) 2010-01-14

Family

ID=27613372

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60330346T Expired - Lifetime DE60330346D1 (de) 2002-01-16 2003-01-16 Mehrfachsubstituierte proteasevarianten

Country Status (13)

Country Link
US (8) US7306937B2 (de)
EP (1) EP1523553B1 (de)
JP (2) JP4703113B2 (de)
AT (1) ATE450606T1 (de)
AU (1) AU2003210552A1 (de)
BR (1) BRPI0306955B1 (de)
CA (2) CA2894330A1 (de)
DE (1) DE60330346D1 (de)
DK (1) DK1523553T3 (de)
ES (1) ES2336092T3 (de)
MX (1) MXPA04006809A (de)
PT (1) PT1523553E (de)
WO (1) WO2003062381A2 (de)

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CN103820423A (zh) * 2004-04-02 2014-05-28 诺维信公司 具有改变的免疫原性的枯草杆菌酶变体
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EP3045532A1 (de) * 2006-06-02 2016-07-20 President and Fellows of Harvard College Neugestaltung von proteinoberflächen
JP5785686B2 (ja) 2006-06-23 2015-09-30 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン 複数の性質をエンジニアリングするための部位評価ライブラリーを用いた、配列と活性との関係の系統的な評価
AU2007275864B2 (en) * 2006-07-18 2013-05-02 Danisco Us, Inc., Genencor Division Protease variants active over a broad temperature range
CN101778940B (zh) * 2007-06-06 2017-04-05 丹尼斯科美国公司 改进多种蛋白质性质的方法
WO2009134808A2 (en) * 2008-04-28 2009-11-05 President And Fellows Of Harvard College Supercharged proteins for cell penetration
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EP2362896A2 (de) * 2008-11-11 2011-09-07 Danisco US Inc. Bacillus subtilisin mit mindestens einer kombinierbaren mutation
BRPI0922084B1 (pt) * 2008-11-11 2020-12-29 Danisco Us Inc. variante de subtilisina isolada de uma subtilisina de bacillus e sua composição de limpeza
JP2012525146A (ja) 2009-04-28 2012-10-22 プレジデント アンド フェロウズ オブ ハーバード カレッジ 細胞透過のための過剰に荷電されたタンパク質
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US8728790B2 (en) 2009-12-09 2014-05-20 Danisco Us Inc. Compositions and methods comprising protease variants
CN105925555B (zh) * 2010-05-06 2020-12-22 丹尼斯科美国公司 包含枯草杆菌蛋白酶变体的组合物和方法
FI123942B (fi) 2010-10-29 2013-12-31 Ab Enzymes Oy Sieniperäisen seriiniproteaasin variantteja
DE102011007313A1 (de) 2011-04-13 2012-10-18 Henkel Ag & Co. Kgaa Expressionsverfahren
WO2012151480A2 (en) * 2011-05-05 2012-11-08 The Procter & Gamble Company Compositions and methods comprising serine protease variants
CN104011204A (zh) * 2011-12-20 2014-08-27 诺维信公司 枯草杆菌酶变体和编码它们的多核苷酸
CN108291179B (zh) 2015-11-25 2020-07-07 荷兰联合利华有限公司 液体洗涤剂组合物
EP3702452A1 (de) * 2019-03-01 2020-09-02 Novozymes A/S Waschmittelzusammensetzungen mit zwei proteasen
CN110846299B (zh) * 2019-11-22 2021-09-24 江南大学 一种前导肽突变体及其在角蛋白酶生产中的应用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

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Also Published As

Publication number Publication date
US20080124783A1 (en) 2008-05-29
AU2003210552A1 (en) 2003-09-02
US20090011489A1 (en) 2009-01-08
CA2472725C (en) 2015-08-11
US20050221461A1 (en) 2005-10-06
ATE450606T1 (de) 2009-12-15
WO2003062381A3 (en) 2005-02-24
JP2011041571A (ja) 2011-03-03
EP1523553A4 (de) 2006-06-21
ES2336092T3 (es) 2010-04-08
BRPI0306955B1 (pt) 2016-03-22
CA2472725A1 (en) 2003-07-31
US20080176313A1 (en) 2008-07-24
EP1523553B1 (de) 2009-12-02
EP1523553A2 (de) 2005-04-20
US20130171717A1 (en) 2013-07-04
BR0306955A (pt) 2006-12-19
US7306937B2 (en) 2007-12-11
PT1523553E (pt) 2010-03-03
CA2894330A1 (en) 2003-07-31
US20110091958A1 (en) 2011-04-21
MXPA04006809A (es) 2004-10-11
JP2005519592A (ja) 2005-07-07
US20110086412A1 (en) 2011-04-14
DK1523553T3 (da) 2010-04-19
JP4703113B2 (ja) 2011-06-15
US20110091959A1 (en) 2011-04-21
WO2003062381A2 (en) 2003-07-31

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