JP4473878B2 - 核酸を増幅および配列決定する方法 - Google Patents

核酸を増幅および配列決定する方法 Download PDF

Info

Publication number
JP4473878B2
JP4473878B2 JP2006532273A JP2006532273A JP4473878B2 JP 4473878 B2 JP4473878 B2 JP 4473878B2 JP 2006532273 A JP2006532273 A JP 2006532273A JP 2006532273 A JP2006532273 A JP 2006532273A JP 4473878 B2 JP4473878 B2 JP 4473878B2
Authority
JP
Japan
Prior art keywords
dna
sequencing
nucleic acid
beads
primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2006532273A
Other languages
English (en)
Japanese (ja)
Other versions
JP2007523627A (ja
JP2007523627A6 (ja
Inventor
ジョン エイチ． リーモン
ケントン ローマン
ジョナサン エム． ロスバーグ
マイケル ピー． ウェイナー
Original Assignee
４５４ コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32854649&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP4473878(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by ４５４ コーポレーション filed Critical ４５４ コーポレーション
Publication of JP2007523627A publication Critical patent/JP2007523627A/ja
Publication of JP2007523627A6 publication Critical patent/JP2007523627A6/ja
Application granted granted Critical
Publication of JP4473878B2 publication Critical patent/JP4473878B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/12—Measuring magnetic properties of articles or specimens of solids or fluids
    • G01R33/1269—Measuring magnetic properties of articles or specimens of solids or fluids of molecules labeled with magnetic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Photoreceptors In Electrophotography (AREA)
  • Colloid Chemistry (AREA)
JP2006532273A 2003-01-29 2004-01-28 核酸を増幅および配列決定する方法 Expired - Lifetime JP4473878B2 (ja)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US44347103P 2003-01-29 2003-01-29
US60/443,471 2003-01-29
US46507103P 2003-04-23 2003-04-23
US60/465,071 2003-04-23
US47650403P 2003-06-06 2003-06-06
US47660203P 2003-06-06 2003-06-06
US47631303P 2003-06-06 2003-06-06
US47659203P 2003-06-06 2003-06-06
US60/476,592 2003-06-06
US60/476,602 2003-06-06
US60/476,504 2003-06-06
US60/476,313 2003-06-06
US49798503P 2003-08-25 2003-08-25
US60/497,985 2003-08-25
PCT/US2004/002570 WO2005003375A2 (en) 2003-01-29 2004-01-28 Methods of amplifying and sequencing nucleic acids

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2009297745A Division JP2010142233A (ja) 2003-01-29 2009-12-28 核酸を増幅および配列決定する方法

Publications (3)

Publication Number Publication Date
JP2007523627A JP2007523627A (ja) 2007-08-23
JP2007523627A6 JP2007523627A6 (ja) 2008-02-14
JP4473878B2 true JP4473878B2 (ja) 2010-06-02

Family

ID=32854649

Family Applications (9)

Application Number Title Priority Date Filing Date
JP2006532273A Expired - Lifetime JP4473878B2 (ja) 2003-01-29 2004-01-28 核酸を増幅および配列決定する方法
JP2006503133A Expired - Lifetime JP4768598B2 (ja) 2003-01-29 2004-01-28 ビーズエマルジョン核酸増幅
JP2006503163A Pending JP2007525151A (ja) 2003-01-29 2004-01-28 一本鎖ｄｎａライブラリーの調製方法
JP2006503134A Expired - Fee Related JP4480715B2 (ja) 2003-01-29 2004-01-28 二重末端シーケンシング
JP2009297745A Pending JP2010142233A (ja) 2003-01-29 2009-12-28 核酸を増幅および配列決定する方法
JP2010053827A Expired - Lifetime JP5258822B2 (ja) 2003-01-29 2010-03-10 ビーズエマルジョン核酸増幅
JP2012181295A Expired - Lifetime JP5495399B2 (ja) 2003-01-29 2012-08-20 核酸を増幅および配列決定する方法
JP2012258136A Expired - Lifetime JP5726155B2 (ja) 2003-01-29 2012-11-27 ビーズエマルジョン核酸増幅
JP2012282220A Pending JP2013090635A (ja) 2003-01-29 2012-12-26 ビーズエマルジョン核酸増幅

Family Applications After (8)

Application Number Title Priority Date Filing Date
JP2006503133A Expired - Lifetime JP4768598B2 (ja) 2003-01-29 2004-01-28 ビーズエマルジョン核酸増幅
JP2006503163A Pending JP2007525151A (ja) 2003-01-29 2004-01-28 一本鎖ｄｎａライブラリーの調製方法
JP2006503134A Expired - Fee Related JP4480715B2 (ja) 2003-01-29 2004-01-28 二重末端シーケンシング
JP2009297745A Pending JP2010142233A (ja) 2003-01-29 2009-12-28 核酸を増幅および配列決定する方法
JP2010053827A Expired - Lifetime JP5258822B2 (ja) 2003-01-29 2010-03-10 ビーズエマルジョン核酸増幅
JP2012181295A Expired - Lifetime JP5495399B2 (ja) 2003-01-29 2012-08-20 核酸を増幅および配列決定する方法
JP2012258136A Expired - Lifetime JP5726155B2 (ja) 2003-01-29 2012-11-27 ビーズエマルジョン核酸増幅
JP2012282220A Pending JP2013090635A (ja) 2003-01-29 2012-12-26 ビーズエマルジョン核酸増幅

Country Status (10)

Country Link
US (15) US7244567B2 (enExample)
EP (8) EP1594950A4 (enExample)
JP (9) JP4473878B2 (enExample)
CN (2) CN101128601B (enExample)
AT (4) ATE437945T1 (enExample)
AU (4) AU2004254552B2 (enExample)
CA (6) CA2513541A1 (enExample)
DE (4) DE602004036672C5 (enExample)
ES (5) ES2396245T3 (enExample)
WO (4) WO2005003375A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9897986B2 (en) 2013-10-29 2018-02-20 Regal Beloit America, Inc. System and method for enabling a motor controller to communicate using multiple different communication protocols

Families Citing this family (1275)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
DK1019496T3 (da) 1997-07-07 2005-01-10 Medical Res Council In vitro-sorteringsmetode
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
CA2290731A1 (en) * 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
EP1356094B1 (en) * 2000-06-26 2010-01-13 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
CA2430329A1 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
NZ523167A (en) 2001-03-09 2006-08-31 Nugen Technologies Inc Methods, compositions and kits for amplifying RNA sequences of interest which employ an RNA/DNA composite primer
EP1401850A1 (en) * 2001-06-20 2004-03-31 Nuevolution A/S Nucleoside derivatives for library preparation
US20030096268A1 (en) * 2001-07-06 2003-05-22 Michael Weiner Method for isolation of independent, parallel chemical micro-reactions using a porous filter
DE10147074A1 (de) * 2001-09-25 2003-05-08 Beru Ag Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung
US20030108664A1 (en) * 2001-10-05 2003-06-12 Kodas Toivo T. Methods and compositions for the formation of recessed electrical features on a substrate
JP4192097B2 (ja) * 2001-10-25 2008-12-03 バル−イラン ユニバーシティ 相互作用型透明個別細胞バイオチッププロセッサー
GB0127564D0 (en) * 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7413854B2 (en) * 2002-03-15 2008-08-19 Nuevolution A/S Method for synthesising templated molecules
US20030217923A1 (en) * 2002-05-24 2003-11-27 Harrison D. Jed Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US10730906B2 (en) * 2002-08-01 2020-08-04 Nuevolutions A/S Multi-step synthesis of templated molecules
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
AU2003266932B2 (en) * 2002-09-27 2009-04-09 Mpm-Holding Aps Spatially encoded polymer matrix
EP1558744B1 (en) 2002-10-30 2011-06-15 Nuevolution A/S Enzymatic encoding
WO2004056994A2 (en) 2002-12-19 2004-07-08 Nuevolution A/S Quasirandom structure and function guided synthesis methods
CA2512071A1 (en) * 2002-12-30 2004-07-22 The Regents Of The University Of California Methods and apparatus for pathogen detection and analysis
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
EP1594950A4 (en) 2003-01-29 2007-07-25 454 Corp PRODUCT FOR THE PREPARATION OF SIMPLE STRANDED DNA BANKS
US20070026397A1 (en) 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
AU2004214891B2 (en) 2003-02-26 2010-01-07 Complete Genomics, Inc. Random array DNA analysis by hybridization
IL154677A0 (en) * 2003-02-27 2003-09-17 Univ Bar Ilan A method and apparatus for manipulating an individual cell
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) * 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
FR2856498B1 (fr) * 2003-06-19 2005-09-30 Goulven Jean Alain Vernois Distribution sous atmosphere controlee
US8597597B2 (en) * 2003-06-26 2013-12-03 Seng Enterprises Ltd. Picoliter well holding device and method of making the same
US9200245B2 (en) 2003-06-26 2015-12-01 Seng Enterprises Ltd. Multiwell plate
US7888110B2 (en) * 2003-06-26 2011-02-15 Seng Enterprises Ltd. Pico liter well holding device and method of making the same
CA2531105C (en) * 2003-07-05 2015-03-17 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
EP1670939B1 (en) * 2003-09-18 2009-11-04 Nuevolution A/S A method for obtaining structural information concerning an encoded molecule and method for selecting compounds
WO2005028109A2 (en) * 2003-09-19 2005-03-31 Applera Corporation Microplates useful for conducting thermocycled nucleotide amplification
WO2005054431A2 (en) * 2003-12-01 2005-06-16 454 Corporation Method for isolation of independent, parallel chemical micro-reactions using a porous filter
US7972994B2 (en) 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
EP2789383B1 (en) 2004-01-07 2023-05-03 Illumina Cambridge Limited Molecular arrays
WO2005073410A2 (en) 2004-01-28 2005-08-11 454 Corporation Nucleic acid amplification with continuous flow emulsion
WO2005078122A2 (en) * 2004-02-17 2005-08-25 Nuevolution A/S Method for enrichment involving elimination by mismatch hybridisation
EP2290106B1 (en) * 2004-03-08 2018-01-03 Rubicon Genomics, Inc. Method for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7622281B2 (en) * 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
WO2005118773A2 (en) 2004-05-28 2005-12-15 Wafergen, Inc. Apparatus and methods for multiplex analyses
US7799553B2 (en) * 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US7264934B2 (en) * 2004-06-10 2007-09-04 Ge Healthcare Bio-Sciences Corp. Rapid parallel nucleic acid analysis
US7692219B1 (en) 2004-06-25 2010-04-06 University Of Hawaii Ultrasensitive biosensors
US20080063251A1 (en) * 2004-07-07 2008-03-13 Mordechai Deutsch Method and Device for Identifying an Image of a Well in an Image of a Well-Bearing
EP1781404A2 (en) * 2004-08-25 2007-05-09 Seng Enterprises Limited Method and device for isolating cells
WO2006032044A2 (en) 2004-09-15 2006-03-23 Microchip Biotechnologies, Inc. Microfluidic devices
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060110764A1 (en) * 2004-10-25 2006-05-25 Tom Tang Large-scale parallelized DNA sequencing
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
US7485451B2 (en) * 2004-11-18 2009-02-03 Regents Of The University Of California Storage stable compositions of biological materials
JP2008522628A (ja) * 2004-12-11 2008-07-03 サイトジェニックス， インコーポレイテッド 高品質核酸のセルフリー生合成及びその使用
DE602005023534D1 (de) 2005-01-25 2010-10-21 Seng Entpr Ltd Mikrofluidische vorrichtung zur untersuchung zellen
EP2857523A1 (en) 2005-02-01 2015-04-08 Applied Biosystems, LLC Method for identifying a sequence in a polynucleotide
JP2006211984A (ja) * 2005-02-04 2006-08-17 Univ Nagoya エマルジョンを利用した核酸増幅方法、及び核酸増幅反応用キット
US8374887B1 (en) 2005-02-11 2013-02-12 Emily H. Alexander System and method for remotely supervising and verifying pharmacy functions
EP1848819A4 (en) * 2005-02-16 2010-01-06 Genetic Technologies Ltd GENETIC ANALYSIS METHODS COMPRISING THE AMPLIFICATION OF COMPLEMENTARY DUPLICONS
US7932081B2 (en) * 2005-03-10 2011-04-26 Gen-Probe Incorporated Signal measuring system for conducting real-time amplification assays
US7604940B1 (en) 2005-03-16 2009-10-20 Applied Biosystems, Llc Compositions and methods for analyzing isolated polynucleotides
WO2006099579A2 (en) * 2005-03-16 2006-09-21 Applera Corporation Compositions and methods for clonal amplification and analysis of polynucleotides
US20060228721A1 (en) 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
JP2008538496A (ja) * 2005-04-12 2008-10-30 ４５４ ライフ サイエンシーズ コーポレイション ウルトラディープ配列決定を用いて配列変異体を決定するための方法
CN101213311B (zh) * 2005-04-29 2013-02-13 合成基因组股份有限公司 利用滚环扩增扩增和克隆单个dna分子
DK1885882T3 (da) * 2005-05-10 2011-04-11 State Of Oregon Acting By & Through The State Board Of Higher Eduction On Behalf Of The University O Fremgangsmåder til kortlægning af polymorfier og polymorfi-mikroarray
CA2615323A1 (en) * 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
US20090233291A1 (en) * 2005-06-06 2009-09-17 454 Life Sciences Corporation Paired end sequencing
SI1910538T1 (sl) 2005-06-09 2011-09-30 Praecis Pharm Inc POSTOPKI ZA SINTEZO KODIRANIH KNJIĹľNIC
CA2611671C (en) 2005-06-15 2013-10-08 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US20090264299A1 (en) * 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
ES2387878T3 (es) 2005-06-23 2012-10-03 Keygene N.V. Estrategias para la identificación de alto rendimiento y la detección de polimorfismos
ATE465274T1 (de) * 2005-06-23 2010-05-15 Keygene Nv Verbesserte strategien zur sequenzierung komplexer genome unter verwendung von sequenziertechniken mit hohem durchsatz
US20070031865A1 (en) * 2005-07-07 2007-02-08 David Willoughby Novel Process for Construction of a DNA Library
GB0514935D0 (en) * 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10083273B2 (en) * 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
CA2619686A1 (en) * 2005-08-19 2007-02-22 Commomwealth Scientific And Industrial Research Organisation Arachnocampa luciferases
WO2007024800A2 (en) 2005-08-22 2007-03-01 Applera Corporation Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
JP2009508495A (ja) * 2005-09-16 2009-03-05 ４５４ ライフ サイエンシーズ コーポレイション ｃＤＮＡライブラリー調製
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
JP5237099B2 (ja) 2005-09-29 2013-07-17 キージーン ナムローゼ フェンノートシャップ 変異させた集団のハイスループットスクリーニング
CA2624896C (en) 2005-10-07 2017-11-07 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
WO2007133831A2 (en) 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
US20070141555A1 (en) * 2005-10-11 2007-06-21 Mordechai Deutsch Current damper for the study of cells
PT1948816E (pt) * 2005-10-24 2012-03-19 Univ Johns Hopkins Processos melhorados de focalização
KR100828714B1 (ko) 2005-10-25 2008-05-09 주식회사 엘지화학 리보핵산을 이용한 멀티플렉스 증폭방법
US7989395B2 (en) * 2005-10-28 2011-08-02 Glaxosmithkline Llc Methods for identifying compounds of interest using encoded libraries
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US8288120B2 (en) * 2005-11-03 2012-10-16 Seng Enterprises Ltd. Method for studying floating, living cells
JP2009515192A (ja) * 2005-11-08 2009-04-09 インコム，インコーポレイテッド 光ファイバ探査型マイクロスライド、マイクロスライドキット、およびその使用
AU2006312378B2 (en) * 2005-11-14 2012-02-16 Keygene N.V. Method for high throughput screening of transposon tagging populations and massive parallel sequence identification of insertion sites
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
EP2341140B1 (en) 2005-12-01 2017-07-19 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
DK1966394T3 (da) * 2005-12-22 2012-10-29 Keygene Nv Forbedrede strategier til transkriptprofilering under anvendelse af high throughput-sekventeringsteknologier
CN101374963B (zh) 2005-12-22 2014-06-04 凯津公司 用于基于aflp的高通量多态性检测的方法
EP1984738A2 (en) 2006-01-11 2008-10-29 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US20090203148A1 (en) * 2006-01-19 2009-08-13 Vera Gorfinkel Methods and Devices For Detection and Identification of Encoded Beads and Biological Molecules
CN101360819A (zh) * 2006-01-20 2009-02-04 凸版印刷株式会社 反应容器及dna的扩增反应方法
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
US7749365B2 (en) 2006-02-01 2010-07-06 IntegenX, Inc. Optimized sample injection structures in microfluidic separations
CA2641271A1 (en) 2006-02-03 2008-03-13 Microchip Biotechnologies, Inc. Microfluidic devices
DE102006005287B4 (de) * 2006-02-06 2012-12-27 Siemens Ag Verfahren zum Nachweis von Zielnukleinsäuren
CA2641851A1 (en) 2006-02-08 2007-08-16 Eric Hans Vermaas Method for sequencing a polynucleotide template
US9012184B2 (en) * 2006-02-08 2015-04-21 Illumina Cambridge Limited End modification to prevent over-representation of fragments
EP2002367B1 (en) 2006-02-16 2017-03-29 454 Life Sciences Corporation System and method for correcting primer extension errors in nucleic acid sequence data
US8364417B2 (en) * 2007-02-15 2013-01-29 454 Life Sciences Corporation System and method to correct out of phase errors in DNA sequencing data by use of a recursive algorithm
US8492098B2 (en) * 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
US11237171B2 (en) 2006-02-21 2022-02-01 Trustees Of Tufts College Methods and arrays for target analyte detection and determination of target analyte concentration in solution
US7766033B2 (en) * 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
EP4105644A3 (en) 2006-03-31 2022-12-28 Illumina, Inc. Systems and devices for sequence by synthesis analysis
JP5389638B2 (ja) 2006-04-04 2014-01-15 キージーン ナムローゼ フェンノートシャップ 制限断片に基づく分子マーカーのハイスループットな検出
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
CN101495654A (zh) * 2006-04-19 2009-07-29 阿普里拉股份有限公司 无凝胶珠基测序的试剂、方法和文库
US10522240B2 (en) 2006-05-03 2019-12-31 Population Bio, Inc. Evaluating genetic disorders
US7702468B2 (en) 2006-05-03 2010-04-20 Population Diagnostics, Inc. Evaluating genetic disorders
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
WO2007133710A2 (en) * 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
WO2007136736A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Methods for nucleic acid sorting and synthesis
US20080124726A1 (en) * 2006-05-26 2008-05-29 Althea Technologies, Inc. Biochemical analysis of partitioned cells
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8569416B2 (en) 2006-06-06 2013-10-29 Dow Corning Corporation Single phase silicone acrylate formulation
EP2024406B1 (en) * 2006-06-06 2012-08-01 Dow Corning Corporation A silicone acrylate hybrid composition
US8614278B2 (en) 2006-06-06 2013-12-24 Dow Corning Corporation Silicone acrylate hybrid composition and method of making same
US20080124707A1 (en) * 2006-06-09 2008-05-29 Agency For Science, Technology And Research Nucleic acid concatenation
EP2366801B1 (en) 2006-06-14 2016-10-19 Verinata Health, Inc Methods for the diagnosis of fetal abnormalities
US20080009007A1 (en) * 2006-06-16 2008-01-10 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
EP2821506A1 (en) 2006-07-12 2015-01-07 Keygene N.V. Identification of clones by sequencing of pool-specific adapters
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US9328378B2 (en) 2006-07-31 2016-05-03 Illumina Cambridge Limited Method of library preparation avoiding the formation of adaptor dimers
EP2077912B1 (en) 2006-08-07 2019-03-27 The President and Fellows of Harvard College Fluorocarbon emulsion stabilizing surfactants
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
WO2008039998A2 (en) * 2006-09-28 2008-04-03 President And Fellows Of Harvard College Methods for sequencing dna
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008045575A2 (en) * 2006-10-13 2008-04-17 J. Craig Venter Institute, Inc. Sequencing method
US8841116B2 (en) * 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
WO2008058018A2 (en) 2006-11-02 2008-05-15 Mayo Foundation For Medical Education And Research Predicting cancer outcome
CN103173346B (zh) * 2006-11-06 2017-04-19 科隆迪亚戈有限公司 使用结合元件用于分析的装置和方法
US20090111706A1 (en) * 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
EP2518162B1 (en) * 2006-11-15 2018-03-07 Biospherex LLC Multitag sequencing and ecogenomics analysis
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US20080145898A1 (en) * 2006-12-14 2008-06-19 Applera Corporation Sequencing methods
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
AU2007334393A1 (en) * 2006-12-14 2008-06-26 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
EP2109666A4 (en) * 2007-02-05 2011-09-14 Integenx Inc MICROFLUIDIC AND NANOFLUIDIC DEVICES, SYSTEMS, AND APPLICATIONS
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US9152150B1 (en) 2007-02-22 2015-10-06 Applied Biosystems, Llc Compositions, systems, and methods for immiscible fluid discrete volume manipulation
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US8617816B2 (en) 2007-03-16 2013-12-31 454 Life Sciences, A Roche Company System and method for detection of HIV drug resistant variants
US20100151465A1 (en) 2008-03-27 2010-06-17 Jingyue Ju Selective Capture and Release of Analytes
US20080243865A1 (en) * 2007-03-28 2008-10-02 Oracle International Corporation Maintaining global state of distributed transaction managed by an external transaction manager for clustered database systems
US20080239867A1 (en) * 2007-03-28 2008-10-02 Gilbert Donna J Adjustable stir
JP2008245612A (ja) * 2007-03-30 2008-10-16 Hitachi Ltd 試料調製法および装置
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US7883265B2 (en) * 2007-06-01 2011-02-08 Applied Biosystems, Llc Devices, systems, and methods for preparing emulsions
CN101720359A (zh) * 2007-06-01 2010-06-02 454生命科学公司 从多重混合物中识别个别样本的系统和方法
CN101802218A (zh) * 2007-06-28 2010-08-11 454生命科学公司 在核酸测序中用于自适应试剂控制的系统和方法
EP2395113A1 (en) * 2007-06-29 2011-12-14 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
US8454906B2 (en) * 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
EP2173909A1 (en) 2007-07-26 2010-04-14 Roche Diagnostics GmbH Target preparation for parallel sequencing of complex genomes
JP5020734B2 (ja) * 2007-07-31 2012-09-05 株式会社日立ハイテクノロジーズ 核酸解析方法及び装置
EP2201021A4 (en) 2007-08-29 2012-01-25 Applied Biosystems Llc METHOD OF ALTERNATING NUCLEIC ACID SEQUENCING
WO2009032167A1 (en) * 2007-08-29 2009-03-12 Illumina Cambridge Method for sequencing a polynucleotide template
ATE549419T1 (de) 2007-08-29 2012-03-15 Sequenom Inc Verfahren und zusammensetzungen für die universelle grössenspezifische polymerasekettenreaktion
WO2009029073A1 (en) 2007-08-30 2009-03-05 The Trustees Of Tufts College Methods for determining the concentration of an analyte in solution.
WO2009035623A1 (en) * 2007-09-11 2009-03-19 Arryx, Inc. Binding method and apparatus for sorting objects
WO2009036192A1 (en) * 2007-09-13 2009-03-19 Applied Precision, Inc. Method of dispersing immersion liquid on a specimen substrate for high resolution imaging and lithographie
EP2185261A4 (en) * 2007-09-13 2013-08-28 Arryx Inc METHODS AND APPARATUSES FOR SORTING OBJECTS IN MEDICO-LEGAL ANALYSIS OF RNA AND IN MEDICAL DIAGNOSES
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
EP2188386A2 (en) * 2007-09-17 2010-05-26 Universite De Strasbourg Method for detecting or quantifying a truncating mutation
US20090118129A1 (en) * 2007-09-28 2009-05-07 Pacific Biosciences Of California, Inc. Virtual reads for readlength enhancement
WO2009046149A1 (en) * 2007-10-01 2009-04-09 Applied Biosystems Inc. Chase ligation sequencing
US20100086914A1 (en) * 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
EP2053132A1 (en) 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
US20110015084A1 (en) * 2007-10-25 2011-01-20 Monsanto Technology Llc Methods for Identifying Genetic Linkage
US9975118B2 (en) * 2007-11-15 2018-05-22 Seng Enterprises Ltd. Device for the study of living cells
US9145540B1 (en) 2007-11-15 2015-09-29 Seng Enterprises Ltd. Device for the study of living cells
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
CN101946010B (zh) 2007-12-21 2014-08-20 哈佛大学 用于核酸测序的系统和方法
US8189186B2 (en) * 2007-12-27 2012-05-29 Lawrence Livermore National Security, Llc. Signal enhancement using a switchable magnetic trap
US20090181390A1 (en) * 2008-01-11 2009-07-16 Signosis, Inc. A California Corporation High throughput detection of micrornas and use for disease diagnosis
EP3699291B1 (en) * 2008-01-17 2024-08-07 Sequenom, Inc. Single molecule nucleic acid sequence analysis processes and compositions
EP2234916A4 (en) 2008-01-22 2016-08-10 Integenx Inc UNIVERSAL SPECIMEN PREPARATION SYSTEM AND ITS USE IN AN INTEGRATED ANALYSIS SYSTEM
US7767400B2 (en) * 2008-02-03 2010-08-03 Helicos Biosciences Corporation Paired-end reads in sequencing by synthesis
US20090203086A1 (en) * 2008-02-06 2009-08-13 454 Life Sciences Corporation System and method for improved signal detection in nucleic acid sequencing
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
WO2009102896A2 (en) * 2008-02-12 2009-08-20 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
US8709726B2 (en) * 2008-03-11 2014-04-29 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
BRPI0908734A2 (pt) 2008-03-17 2015-07-28 Expressive Res Bv Métodos para a identificação de dna genômico em uma amostra e para a indentificação de polimorfismos, e, kit
US10745740B2 (en) * 2008-03-19 2020-08-18 Qiagen Sciences, Llc Sample preparation
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
EP3045542B1 (en) * 2008-03-28 2016-11-16 Pacific Biosciences of California, Inc. Methods for nucleic acid sequencing
US8557742B2 (en) * 2008-04-04 2013-10-15 Novozymes A/S Method of enhancing enzyme activity
JP5227062B2 (ja) 2008-04-08 2013-07-03 株式会社日立製作所 Ｄｎａ分析装置
TWI460602B (zh) * 2008-05-16 2014-11-11 Counsyl Inc 廣用的懷孕前篩檢裝置
EP2291553A4 (en) 2008-05-28 2011-12-14 Genomedx Biosciences Inc SYSTEMS AND METHODS FOR THE EXPRESSION-BASED DISTINCTION OF DIFFERENT CLINICAL ILLICIT STADIAS IN PROSTATE CANCER
US10407731B2 (en) 2008-05-30 2019-09-10 Mayo Foundation For Medical Education And Research Biomarker panels for predicting prostate cancer outcomes
CN102203282B (zh) 2008-06-25 2014-04-30 生命技术公司 使用大规模fet阵列测量分析物的方法和装置
US7888034B2 (en) 2008-07-01 2011-02-15 454 Life Sciences Corporation System and method for detection of HIV tropism variants
WO2010003132A1 (en) 2008-07-02 2010-01-07 Illumina Cambridge Ltd. Using populations of beads for the fabrication of arrays on surfaces
WO2010009426A2 (en) * 2008-07-17 2010-01-21 Life Technologies Corporation Devices and methods for reagent delivery
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
EP2315850B1 (en) 2008-07-18 2015-02-25 TrovaGene, Inc. Methods for pcr-based detection of "ultra short" nucleic acid sequences
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US20100035252A1 (en) * 2008-08-08 2010-02-11 Ion Torrent Systems Incorporated Methods for sequencing individual nucleic acids under tension
US8808986B2 (en) 2008-08-27 2014-08-19 Gen9, Inc. Methods and devices for high fidelity polynucleotide synthesis
US8795961B2 (en) 2008-09-05 2014-08-05 Pacific Biosciences Of California, Inc. Preparations, compositions, and methods for nucleic acid sequencing
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US20100075862A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation High sensitivity determination of the concentration of analyte molecules or particles in a fluid sample
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US20100184045A1 (en) * 2008-09-23 2010-07-22 Helicos Biosciences Corporation Methods for sequencing degraded or modified nucleic acids
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US8222047B2 (en) * 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
US20100075439A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation Ultra-sensitive detection of molecules by capture-and-release using reducing agents followed by quantification
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
CA2738578C (en) 2008-09-23 2021-05-04 Quantalife, Inc. Droplet-based assay system
WO2011120006A1 (en) 2010-03-25 2011-09-29 Auantalife, Inc. A Delaware Corporation Detection system for droplet-based assays
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
US20100261189A1 (en) * 2008-10-03 2010-10-14 Roche Molecular Systems, Inc. System and method for detection of HLA Variants
US20100087325A1 (en) * 2008-10-07 2010-04-08 Illumina, Inc. Biological sample temperature control system and method
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
WO2010048337A2 (en) 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
EP2664678B1 (en) 2008-10-24 2014-10-08 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2010062546A1 (en) * 2008-10-27 2010-06-03 Qiagen Gaithersburg Inc. Fast results hybrid capture assay on an automated platform
CA2742298C (en) * 2008-11-03 2019-09-10 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
JP2010110262A (ja) * 2008-11-06 2010-05-20 Hitachi Maxell Ltd ウェルプレートを用いた核酸増幅法
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US8691510B2 (en) 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
CN102272327B (zh) 2008-11-07 2015-11-25 赛昆塔公司 通过序列分析监测状况的方法
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
CA2743473C (en) * 2008-11-17 2022-06-14 Veracyte, Inc. Methods and compositions of molecular profiling for disease diagnostics
US9495515B1 (en) 2009-12-09 2016-11-15 Veracyte, Inc. Algorithms for disease diagnostics
US10236078B2 (en) 2008-11-17 2019-03-19 Veracyte, Inc. Methods for processing or analyzing a sample of thyroid tissue
MX2011005872A (es) 2008-12-05 2011-08-17 Keygene Nv Farneseno sintasa.
EP3290531B1 (en) 2008-12-19 2019-07-24 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
US8236532B2 (en) * 2008-12-23 2012-08-07 Illumina, Inc. Multibase delivery for long reads in sequencing by synthesis protocols
US8672532B2 (en) 2008-12-31 2014-03-18 Integenx Inc. Microfluidic methods
ES2403312T3 (es) 2009-01-13 2013-05-17 Keygene N.V. Nuevas estrategias para la secuenciación del genoma
DK2387627T3 (en) 2009-01-15 2016-07-04 Adaptive Biotechnologies Corp Adaptive immunity profiling and methods for producing monoclonal antibodies
US20100179074A1 (en) * 2009-01-15 2010-07-15 Honeywell International Inc. Methods and apparatus for fluid delivery and removal of micron scale structures
CN102333891A (zh) * 2009-01-20 2012-01-25 利兰·斯坦福青年大学托管委员会 用于药靶诊断、预后和鉴别的单细胞基因表达
US20100331204A1 (en) 2009-02-13 2010-12-30 Jeff Jeddeloh Methods and systems for enrichment of target genomic sequences
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US9074258B2 (en) 2009-03-04 2015-07-07 Genomedx Biosciences Inc. Compositions and methods for classifying thyroid nodule disease
US9056299B2 (en) 2009-03-13 2015-06-16 President And Fellows Of Harvard College Scale-up of flow-focusing microfluidic devices
CN102428191A (zh) * 2009-03-18 2012-04-25 塞昆纳姆股份有限公司 热稳定性内切核酸酶在产生报道分子中的应用
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
GB0904957D0 (en) 2009-03-23 2009-05-06 Univ Erasmus Medical Ct Tumour gene profile
GB0904934D0 (en) 2009-03-23 2009-05-06 Geneseqe As Method and apparatus for detecting molecules
WO2010115100A1 (en) 2009-04-03 2010-10-07 L&C Diagment, Inc. Multiplex nucleic acid detection methods and systems
WO2010117457A2 (en) * 2009-04-08 2010-10-14 Applied Biosystems, Llc System comprising dual-sided thermal cycler and emulsion pcr in pouch
US9090663B2 (en) * 2009-04-21 2015-07-28 The Trustees Of Columbia University In The City Of New York Systems and methods for the capture and separation of microparticles
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
AU2010242073C1 (en) 2009-04-30 2015-12-24 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
EP2248914A1 (en) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The use of class IIB restriction endonucleases in 2nd generation sequencing applications
JP6078339B2 (ja) 2009-05-07 2017-02-08 ベラサイト インコーポレイテッド 甲状腺状態の診断のための方法および組成物
US8003329B2 (en) * 2009-05-12 2011-08-23 Becton Dickinson & Company Molecular counting by color-coded micelles
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
EP3663750B1 (en) 2009-05-29 2021-11-03 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8388908B2 (en) 2009-06-02 2013-03-05 Integenx Inc. Fluidic devices with diaphragm valves
EP2438016B1 (en) 2009-06-05 2021-06-02 IntegenX Inc. Universal sample preparation system and use in an integrated analysis system
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
RU2539032C2 (ru) 2009-06-25 2015-01-10 Фред Хатчинсон Кансэр Рисёч Сентер Способ измерения искусственного иммунитета
WO2011002319A2 (en) * 2009-07-02 2011-01-06 Zygem Corporation Limited Combined nucleic acid blocking, extraction, and detection in a single reaction vessel
JP5099455B2 (ja) * 2009-07-16 2012-12-19 独立行政法人科学技術振興機構 エマルジョンを用いたｒｎａの選択的増幅法
WO2011011175A2 (en) * 2009-07-24 2011-01-27 Illumina, Inc. Method for sequencing a polynucleotide template
NZ598226A (en) * 2009-07-29 2013-09-27 Pyrobett Pte Ltd Method and apparatus for conducting an assay
US9416409B2 (en) 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
SI2669387T1 (sl) 2009-08-25 2017-01-31 Illumina, Inc. Metode za izbiro in pomnoževanje polinukleotidov
WO2011028764A2 (en) 2009-09-02 2011-03-10 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
EP2473618B1 (en) 2009-09-02 2015-03-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
US10072287B2 (en) 2009-09-10 2018-09-11 Centrillion Technology Holdings Corporation Methods of targeted sequencing
CA2774618C (en) 2009-09-23 2020-10-27 Celmatix, Inc. Methods and devices for assessing infertility and/or egg quality
EP2473638B1 (en) 2009-09-30 2017-08-09 Natera, Inc. Methods for non-invasive prenatal ploidy calling
EP2486409A1 (en) 2009-10-09 2012-08-15 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
US8609339B2 (en) * 2009-10-09 2013-12-17 454 Life Sciences Corporation System and method for emulsion breaking and recovery of biological elements
CA2778816C (en) 2009-10-27 2018-07-31 President And Fellows Of Harvard College Droplet creation techniques
WO2011050981A2 (en) 2009-10-30 2011-05-05 Roche Diagnostics Gmbh Method for detecting balanced chromosomal aberrations in a genome
US8524450B2 (en) 2009-10-30 2013-09-03 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
WO2011056872A2 (en) * 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
GB2488289A (en) 2009-11-06 2012-08-22 Univ Leland Stanford Junior Non-invasive diagnosis of graft rejection in organ transplant patients
US20120276524A1 (en) * 2009-11-16 2012-11-01 Genomictree, Inc. Genotyping method
JP2013511991A (ja) * 2009-11-25 2013-04-11 クアンタライフ， インコーポレイテッド 遺伝子材料を検出する方法および組成物
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
ES2574055T3 (es) 2009-11-25 2016-06-14 Gen9, Inc. Métodos y aparatos para la reducción de errores en el ADN basada en un chip
US8584703B2 (en) * 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US10446272B2 (en) 2009-12-09 2019-10-15 Veracyte, Inc. Methods and compositions for classification of samples
WO2011071382A1 (en) 2009-12-10 2011-06-16 Keygene N.V. Polymorfphic whole genome profiling
WO2011074960A1 (en) 2009-12-17 2011-06-23 Keygene N.V. Restriction enzyme based whole genome sequencing
US20130004951A1 (en) 2009-12-18 2013-01-03 Keygene N.V. Bulked mutant analysis
EP2516680B1 (en) 2009-12-22 2016-04-06 Sequenom, Inc. Processes and kits for identifying aneuploidy
WO2011079176A2 (en) 2009-12-23 2011-06-30 Raindance Technologies, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
EP2848704B1 (en) 2010-01-19 2018-08-29 Verinata Health, Inc Sequencing methods for prenatal diagnoses
US20120100548A1 (en) 2010-10-26 2012-04-26 Verinata Health, Inc. Method for determining copy number variations
EP2531880B1 (en) 2010-02-01 2016-08-03 Illumina Inc. Focusing methods and optical systems and assemblies using the same
US20110195457A1 (en) * 2010-02-09 2011-08-11 General Electric Company Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US8575124B2 (en) 2010-02-18 2013-11-05 Anthony P. Shuber Compositions and methods for treating cancer
PL2539450T3 (pl) * 2010-02-25 2016-08-31 Advanced Liquid Logic Inc Sposób wytwarzania bibliotek kwasu nukleinowego
WO2011106629A2 (en) 2010-02-26 2011-09-01 Life Technologies Corporation Modified proteins and methods of making and using same
WO2011109379A1 (en) 2010-03-01 2011-09-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
US8415171B2 (en) * 2010-03-01 2013-04-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US9678068B2 (en) * 2010-03-01 2017-06-13 Quanterix Corporation Ultra-sensitive detection of molecules using dual detection methods
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
DE202011003570U1 (de) 2010-03-06 2012-01-30 Illumina, Inc. Systeme und Vorrichtungen zum Detektieren optischer Signale aus einer Probe
US20110311963A1 (en) * 2010-03-16 2011-12-22 Life Technologies Corporation Method and Apparatus for Addressable Flow Cells in Single Molecule Sequencing
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
EP2556170A4 (en) 2010-03-25 2014-01-01 Quantalife Inc TRAPPING TRANSPORT AND DETECTION SYSTEM
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
SI2556171T1 (sl) 2010-04-05 2016-03-31 Prognosys Biosciences, Inc. Prostorsko kodirane biološke analize
US11225655B2 (en) 2010-04-16 2022-01-18 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
US20110269194A1 (en) * 2010-04-20 2011-11-03 Swift Biosciences, Inc. Materials and methods for nucleic acid fractionation by solid phase entrapment and enzyme-mediated detachment
WO2011135041A1 (en) 2010-04-30 2011-11-03 Roche Diagnostics Gmbh System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus
US9930297B2 (en) 2010-04-30 2018-03-27 Becton, Dickinson And Company System and method for acquiring images of medication preparations
EP2388337B1 (en) * 2010-04-30 2014-07-02 Nxp B.V. Sensing device and manufacturing method thereof
SG10201503534RA (en) 2010-05-06 2015-06-29 Sequenta Inc Monitoring health and disease status using clonotype profiles
SG185438A1 (en) * 2010-05-06 2012-12-28 Ibis Biosciences Inc Integrated sample preparation systems and stabilized enzyme mixtures
CA2796822C (en) 2010-05-07 2021-10-05 The Board Of Trustees Of The Leland Standford Junior University Measurement and comparison of immune diversity by high-throughput sequencing
WO2011143194A2 (en) 2010-05-10 2011-11-17 Life Technologies Corporation System and method for processing a biological sample
EP2569626B1 (en) 2010-05-11 2019-11-27 Veracyte, Inc. Methods and compositions for diagnosing conditions
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US8825412B2 (en) 2010-05-18 2014-09-02 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20110287432A1 (en) 2010-05-21 2011-11-24 454 Life Science Corporation System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
EP3290529B1 (en) 2010-06-11 2019-05-22 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
CN103080739B (zh) 2010-06-30 2016-12-21 生命科技公司 用于测试isfet阵列的方法和装置
RU2587606C2 (ru) 2010-06-30 2016-06-20 БиДжиАй Дженомикс Ко., Лтд Новый способ пцр-секвенирования и его применение в генотипировании hla
AU2011226767B1 (en) 2010-06-30 2011-11-10 Life Technologies Corporation Ion-sensing charge-accumulation circuits and methods
US8487790B2 (en) 2010-06-30 2013-07-16 Life Technologies Corporation Chemical detection circuit including a serializer circuit
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
TWI527245B (zh) 2010-07-03 2016-03-21 生命技術公司 具有微摻雜汲極之化學感測器
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
WO2012008831A1 (en) 2010-07-13 2012-01-19 Keygene N.V. Simplified de novo physical map generation from clone libraries
CN103153466B (zh) 2010-07-22 2016-04-13 基因细胞生物系统有限公司 复合液体池
EP2601609B1 (en) 2010-08-02 2017-05-17 Population Bio, Inc. Compositions and methods for discovery of causative mutations in genetic disorders
US20130040375A1 (en) * 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20120034603A1 (en) * 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
EP2606154B1 (en) 2010-08-20 2019-09-25 Integenx Inc. Integrated analysis system
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
CA2810520C (en) 2010-09-10 2019-03-12 Bio-Rad Laboratories, Inc. Size selection of dna for chromatin analysis
EP2617061B1 (en) 2010-09-15 2021-06-30 Life Technologies Corporation Methods and apparatus for measuring analytes
US8685324B2 (en) 2010-09-24 2014-04-01 Life Technologies Corporation Matched pair transistor circuits
WO2012040387A1 (en) 2010-09-24 2012-03-29 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
WO2012050920A1 (en) 2010-09-29 2012-04-19 Illumina, Inc. Compositions and methods for sequencing nucleic acids
US20120077716A1 (en) 2010-09-29 2012-03-29 454 Life Sciences Corporation System and method for producing functionally distinct nucleic acid library ends through use of deoxyinosine
GB201016484D0 (en) 2010-09-30 2010-11-17 Geneseque As Method
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
CN103328981B (zh) 2010-10-04 2017-04-12 吉纳普赛斯股份有限公司 用于自动化可重复使用的平行生物反应的系统和方法
US20120322665A1 (en) 2010-10-08 2012-12-20 454 Life Sciences Corporation System and method for detection of hiv-1 clades and recombinants of the reverse transcriptase and protease regions
WO2012048341A1 (en) 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
US9689012B2 (en) 2010-10-12 2017-06-27 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements
US20150218639A1 (en) 2014-01-17 2015-08-06 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US10093981B2 (en) 2010-10-19 2018-10-09 Northwestern University Compositions and methods for identifying depressive disorders
US20150225792A1 (en) 2014-01-17 2015-08-13 Northwestern University Compositions and methods for identifying depressive disorders
US10233501B2 (en) 2010-10-19 2019-03-19 Northwestern University Biomarkers predictive of predisposition to depression and response to treatment
US8753816B2 (en) 2010-10-26 2014-06-17 Illumina, Inc. Sequencing methods
US10273540B2 (en) 2010-10-27 2019-04-30 Life Technologies Corporation Methods and apparatuses for estimating parameters in a predictive model for use in sequencing-by-synthesis
US9096899B2 (en) 2010-10-27 2015-08-04 Illumina, Inc. Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same
US9387476B2 (en) 2010-10-27 2016-07-12 Illumina, Inc. Flow cells for biological or chemical analysis
EP3141614B1 (en) 2010-10-27 2018-11-28 Life Technologies Corporation Predictive model for use in sequencing-by-synthesis
EP4016086A1 (en) 2010-11-01 2022-06-22 Bio-Rad Laboratories, Inc. System for forming emulsions
WO2012078312A2 (en) 2010-11-12 2012-06-14 Gen9, Inc. Methods and devices for nucleic acids synthesis
EP2637780B1 (en) 2010-11-12 2022-02-09 Gen9, Inc. Protein arrays and methods of using and making the same
US20130267443A1 (en) 2010-11-19 2013-10-10 The Regents Of The University Of Michigan ncRNA AND USES THEREOF
EP2643484A4 (en) 2010-11-22 2014-04-16 Univ California METHOD OF IDENTIFYING AN RNA TRANSCRIPTION OF CELLULAR ORIGIN
US20140011698A1 (en) 2010-12-01 2014-01-09 Morphosys Ag Simultaneous detection of biomolecules in single cells
US9727032B2 (en) 2010-12-14 2017-08-08 Life Technologies Corporation Systems and methods for run-time sequencing run quality monitoring
CA2821906C (en) 2010-12-22 2020-08-25 Natera, Inc. Methods for non-invasive prenatal paternity testing
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
WO2012118555A1 (en) 2010-12-29 2012-09-07 Life Technologies Corporation Time-warped background signal for sequencing-by-synthesis operations
BR112013016708B1 (pt) 2010-12-30 2021-08-17 Foundation Medicine, Inc Otimização de análise multigene de amostras de tumor
US10241075B2 (en) 2010-12-30 2019-03-26 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
WO2013025998A1 (en) 2011-08-18 2013-02-21 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
EP2658999B1 (en) 2010-12-30 2019-03-13 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US20130005591A1 (en) * 2011-01-13 2013-01-03 Halgen Corporation Method for parallel amplification of nucleic acids
CA2823815A1 (en) 2011-01-14 2012-07-19 Keygene N.V. Paired end random sequence based genotyping
WO2012100194A1 (en) 2011-01-20 2012-07-26 Ibis Biosciences, Inc. Microfluidic transducer
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
EP2670863B1 (en) 2011-01-31 2018-06-27 H. Hoffnabb-La Roche Ag Methods of identifying multiple epitopes in cells
WO2012104851A1 (en) 2011-01-31 2012-08-09 Yeda Research And Development Co. Ltd. Methods of diagnosing disease using overlap extension pcr
CA2824387C (en) 2011-02-09 2019-09-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
WO2012129187A1 (en) 2011-03-18 2012-09-27 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US20120244523A1 (en) 2011-03-25 2012-09-27 454 Life Sciences Corporation System and Method for Detection of HIV Integrase Variants
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
US9017993B2 (en) 2011-04-07 2015-04-28 Life Technologies Corporation System and methods for making and processing emulsions
US9121047B2 (en) * 2011-04-07 2015-09-01 Life Technologies Corporation System and methods for making and processing emulsions
EP3366782B1 (en) 2011-04-08 2021-03-10 Life Technologies Corporation Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
US20140302532A1 (en) 2011-04-12 2014-10-09 Quanterix Corporation Methods of determining a treatment protocol for and/or a prognosis of a patient's recovery from a brain injury
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
WO2012149042A2 (en) * 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
CA2834218C (en) 2011-04-29 2021-02-16 Sequenom, Inc. Quantification of a minority nucleic acid species using inhibitory oligonucleotides
WO2012158603A2 (en) 2011-05-13 2012-11-22 Bioo Scientific Corporation Pooled adapter strategy for reducing bias in small rna characterization
EP2710172B1 (en) * 2011-05-20 2017-03-29 Fluidigm Corporation Nucleic acid encoding reactions
BR112013029729A2 (pt) 2011-05-23 2017-01-24 Basf Se controle de emulsões, incluindo emulsões múltiplas
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
EP2714928B1 (en) * 2011-05-27 2017-08-02 Life Technologies Corporation Methods for manipulating biomolecules
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
WO2012169893A1 (en) 2011-06-10 2012-12-13 Stichting Technologisch Topinstituut Groene Genetica (Foundation Technological Top Institute Green Genetics) Transcription factor modulating terpene biosynthesis
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
CN103732744A (zh) 2011-06-15 2014-04-16 Gen9股份有限公司 制备性体外克隆的方法
CA2840929C (en) 2011-07-08 2020-03-24 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
US8920751B2 (en) 2011-07-08 2014-12-30 Life Technologies Corporation Automated enrichment for nucleic acid sequencing
WO2013009927A2 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based assays
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013019714A1 (en) 2011-07-29 2013-02-07 The Trustees Of Columbia University In The City Of New York Mems affinity sensor for continuous monitoring of analytes
WO2013022778A1 (en) 2011-08-05 2013-02-14 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
CA2846197C (en) 2011-08-23 2024-01-16 Foundation Medicine, Inc. Kif5b-ret fusion molecules and uses thereof
IL302248A (en) 2011-08-26 2023-06-01 Gen9 Inc Compositions and methods for high fidelity assembly of nucleic acids
US20150087537A1 (en) 2011-08-31 2015-03-26 Life Technologies Corporation Methods, Systems, Computer Readable Media, and Kits for Sample Identification
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
AU2012304328B2 (en) 2011-09-09 2017-07-20 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
WO2013044217A1 (en) * 2011-09-23 2013-03-28 The Trustees Of Columbia University In The City Of New York Isolation and enrichment of nucleic acids on microchip
WO2013044018A1 (en) 2011-09-23 2013-03-28 Illumina, Inc. Methods and compositions for nucleic acid sequencing
AU2012316218B2 (en) 2011-09-26 2016-03-17 Gen-Probe Incorporated Algorithms for sequence determinations
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
US11008611B2 (en) 2011-09-30 2021-05-18 Unm Rainforest Innovations Double gate ion sensitive field effect transistor
DE102011054101A1 (de) 2011-09-30 2013-04-04 Albert-Ludwigs-Universität Freiburg Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen
US10221454B2 (en) 2011-10-10 2019-03-05 The Hospital For Sick Children Methods and compositions for screening and treating developmental disorders
EP2766498B1 (en) 2011-10-14 2019-06-19 President and Fellows of Harvard College Sequencing by structure assembly
CN102329876B (zh) * 2011-10-14 2014-04-02 深圳华大基因科技有限公司 一种测定待检测样本中疾病相关核酸分子的核苷酸序列的方法
WO2013056178A2 (en) 2011-10-14 2013-04-18 Foundation Medicine, Inc. Novel estrogen receptor mutations and uses thereof
US9228233B2 (en) 2011-10-17 2016-01-05 Good Start Genetics, Inc. Analysis methods
GB2497838A (en) 2011-10-19 2013-06-26 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
HK1201565A1 (en) 2011-10-21 2015-09-04 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
DE102011085473A1 (de) 2011-10-28 2013-05-02 Albert-Ludwigs-Universität Freiburg Verfahren zur Identifikation von Aptameren
US11180807B2 (en) 2011-11-04 2021-11-23 Population Bio, Inc. Methods for detecting a genetic variation in attractin-like 1 (ATRNL1) gene in subject with Parkinson's disease
AU2012336040B2 (en) 2011-11-07 2015-12-10 Illumina, Inc. Integrated sequencing apparatuses and methods of use
CN104094266A (zh) 2011-11-07 2014-10-08 独创系统公司 用于识别原因性基因组变体的方法和系统
GB201120711D0 (en) 2011-12-01 2012-01-11 Univ Erasmus Medical Ct Method for classifying tumour cells
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
CN104105797B (zh) 2011-12-01 2016-08-31 吉纳普赛斯股份有限公司 用于高效电子测序与检测的系统和方法
WO2013085918A1 (en) * 2011-12-05 2013-06-13 The Regents Of The University Of California Methods and compostions for generating polynucleic acid fragments
WO2013085710A2 (en) 2011-12-09 2013-06-13 Illumina, Inc. Expanded radix for polymeric tags
EP2788509B1 (en) 2011-12-09 2018-07-11 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
KR101830778B1 (ko) 2011-12-09 2018-02-22 삼성전자주식회사 발열 입자를 포함하는 오일 층을 이용하는 핵산 증폭 장치 및 방법
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
US10513737B2 (en) 2011-12-13 2019-12-24 Decipher Biosciences, Inc. Cancer diagnostics using non-coding transcripts
US20130189679A1 (en) 2011-12-20 2013-07-25 The Regents Of The University Of Michigan Pseudogenes and uses thereof
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
WO2013096819A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
WO2013096838A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
EP4108782B1 (en) 2011-12-22 2023-06-07 President and Fellows of Harvard College Compositions and methods for analyte detection
US9334491B2 (en) 2011-12-22 2016-05-10 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
US9506113B2 (en) 2011-12-28 2016-11-29 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
US9823246B2 (en) 2011-12-28 2017-11-21 The Board Of Trustees Of The Leland Stanford Junior University Fluorescence enhancing plasmonic nanoscopic gold films and assays based thereon
WO2013102081A2 (en) 2011-12-29 2013-07-04 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
ES2665252T3 (es) 2011-12-30 2018-04-25 Abbott Molecular Inc. Purificación de ácido nucleico de microorganismos a partir de muestras del hospedador
JP2015503923A (ja) 2012-01-09 2015-02-05 オスロ ウニヴェルスィテーツスィーケフース ハーエフＯｓｌｏ Ｕｎｉｖｅｒｓｉｔｅｔｓｓｙｋｅｈｕｓ Ｈｆ 結腸直腸癌の解析のための方法およびバイオマーカー
WO2013106737A1 (en) 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
BR112014018187A8 (pt) 2012-01-25 2017-07-11 Gencell Biosystems Ltd Métodos para manusear líquidos de amostra contendo partículas magnéticas e um líquido de encapsulação, bem como sistema de manuseio de líquido
CA2862552A1 (en) * 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
WO2013116698A2 (en) 2012-02-02 2013-08-08 Invenra, Inc. High throughput screen for biologically active polypeptides
US8597882B2 (en) 2012-02-03 2013-12-03 Pyrobett Pte. Ltd. Method and apparatus for conducting an assay
WO2013117595A2 (en) * 2012-02-07 2013-08-15 Illumina Cambridge Limited Targeted enrichment and amplification of nucleic acids on a support
EP2812452B1 (en) 2012-02-09 2020-05-27 Population Bio, Inc. Methods and compositions for screening and treating developmental disorders
HK1205558A1 (en) 2012-02-21 2015-12-18 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
US20130217023A1 (en) 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
US9605313B2 (en) 2012-03-02 2017-03-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
ES2662128T3 (es) 2012-03-05 2018-04-05 Adaptive Biotechnologies Corporation Determinación de cadenas de receptor inmunitario emparejadas a partir de la frecuencia de subunidades coincidentes
NO2694769T3 (enExample) 2012-03-06 2018-03-03
WO2013132354A2 (en) 2012-03-06 2013-09-12 Oslo Universitetssykehus Hf Gene signatures associated with efficacy of postmastectomy radiotherapy in breast cancer
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
WO2013152114A1 (en) 2012-04-03 2013-10-10 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and crohn's disease
EP2834622B1 (en) 2012-04-03 2023-04-12 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
CN108485940B (zh) * 2012-04-12 2022-01-28 维里纳塔健康公司 拷贝数变异的检测和分类
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
CN114854832A (zh) 2012-04-19 2022-08-05 生命技术公司 核酸扩增
EP2839026B1 (en) 2012-04-19 2016-08-10 Life Technologies Corporation Nucleic acid amplification
WO2013163210A1 (en) * 2012-04-23 2013-10-31 Philip Alexander Rolfe Method and system for detection of an organism
AU2013251701A1 (en) 2012-04-24 2014-10-30 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
CN107617511B (zh) * 2012-04-30 2020-06-12 生命技术公司 离心机装置和系统
EP2844767A4 (en) 2012-05-02 2015-11-18 Ibis Biosciences Inc SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING
US9150905B2 (en) 2012-05-08 2015-10-06 Adaptive Biotechnologies Corporation Compositions and method for measuring and calibrating amplification bias in multiplexed PCR reactions
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
US10192024B2 (en) 2012-05-18 2019-01-29 454 Life Sciences Corporation System and method for generation and use of optimal nucleotide flow orders
WO2013177220A1 (en) * 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
CN102766574B (zh) * 2012-05-24 2013-12-25 中国科学院北京基因组研究所 用于dna测序仪的反应仓
US9617589B2 (en) * 2012-05-25 2017-04-11 The University Of North Carolina At Chapel Hill Microfluidic devices, solid supports for reagents and related methods
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ， インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
EP2864531B2 (en) 2012-06-25 2022-08-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
WO2014008448A1 (en) 2012-07-03 2014-01-09 Sloan Kettering Institute For Cancer Research Quantitative assessment of human t-cell repertoire recovery after allogeneic hematopoietic stem cell transplantation
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US20140093873A1 (en) 2012-07-13 2014-04-03 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
GB201212775D0 (en) * 2012-07-18 2012-08-29 Dna Electronics Ltd Sensing apparatus and method
WO2014015269A1 (en) 2012-07-19 2014-01-23 Ariosa Diagnostics, Inc. Multiplexed sequential ligation-based detection of genetic variants
JP6392222B2 (ja) * 2012-07-24 2018-09-19 ナテラ， インコーポレイテッド 高度多重ｐｃｒ法および組成物
HK1213044A1 (zh) 2012-08-03 2016-06-24 Foundation Medicine, Inc. 人乳头瘤病毒作为肿瘤预後的预测因子
JP6525872B2 (ja) 2012-08-08 2019-06-05 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 細胞中の複数のエピトープを同定するためのダイナミックレンジを高めること
CN110643681A (zh) 2012-08-13 2020-01-03 加利福尼亚大学董事会 用于检测生物组分的方法和系统
EP4397767A3 (en) * 2012-08-14 2024-07-31 10X Genomics, Inc. Microcapsule compositions and methods
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US20140378322A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20150005199A1 (en) * 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US20140378345A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20150005200A1 (en) * 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2885640B1 (en) 2012-08-16 2018-07-18 Genomedx Biosciences, Inc. Prostate cancer prognostics using biomarkers
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP2895620B1 (en) 2012-09-11 2017-08-02 Life Technologies Corporation Nucleic acid amplification
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
WO2014043140A1 (en) * 2012-09-12 2014-03-20 The Regents Of The University Of California Accurate genome sequencing of single cells by single-stranded amplification and sequencing
WO2014043519A1 (en) 2012-09-14 2014-03-20 Population Diagnostics Inc. Methods and compositions for diagnosing, prognosing, and treating neurological conditions
WO2014052855A1 (en) 2012-09-27 2014-04-03 Population Diagnostics, Inc. Methods and compositions for screening and treating developmental disorders
JP6449160B2 (ja) 2012-10-01 2019-01-09 アダプティブ バイオテクノロジーズ コーポレイション 適応免疫受容体多様性およびクローン性特性決定による免疫能力評価関連出願の相互参照
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
US9177098B2 (en) 2012-10-17 2015-11-03 Celmatix Inc. Systems and methods for determining the probability of a pregnancy at a selected point in time
US10162800B2 (en) 2012-10-17 2018-12-25 Celmatix Inc. Systems and methods for determining the probability of a pregnancy at a selected point in time
EP4592400A3 (en) 2012-10-17 2025-10-29 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
EP2912196B1 (en) * 2012-10-26 2018-08-08 Sysmex Corporation Emulsion systems for emulsion-based amplification of nucleic acid
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
EP2912468B1 (en) 2012-10-29 2018-09-12 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
WO2014071358A2 (en) 2012-11-05 2014-05-08 Foundation Medicine, Inc. Novel ntrk1 fusion molecules and uses thereof
AU2013337264B2 (en) 2012-11-05 2018-03-08 Foundation Medicine, Inc. Novel fusion molecules and uses thereof
KR20150090037A (ko) 2012-11-27 2015-08-05 젠셀 바이오시스템즈 리미티드 액체 샘플의 취급
HK1217518A1 (zh) * 2012-12-03 2017-01-13 以琳生物药物有限公司 核酸制备和分析的组合物和方法
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9836577B2 (en) 2012-12-14 2017-12-05 Celmatix, Inc. Methods and devices for assessing risk of female infertility
EP2746405B1 (en) 2012-12-23 2015-11-04 HS Diagnomics GmbH Methods and primer sets for high throughput PCR sequencing
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
WO2014113502A1 (en) 2013-01-15 2014-07-24 Quanterix Corporation Detection of dna or rna using single molecule arrays and other techniques
CN105190656B (zh) 2013-01-17 2018-01-16 佩索纳里斯公司 用于遗传分析的方法和系统
EP3939614A1 (en) 2013-01-18 2022-01-19 Foundation Medicine, Inc. Methods of treating cholangiocarcinoma
US9562269B2 (en) 2013-01-22 2017-02-07 The Board Of Trustees Of The Leland Stanford Junior University Haplotying of HLA loci with ultra-deep shotgun sequencing
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
CA3209385A1 (en) 2013-02-01 2014-08-07 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
CA2902099C (en) 2013-03-08 2020-06-02 F. Hoffmann-La Roche Ag Egfr mutation blood testing
EP3578666A1 (en) 2013-03-12 2019-12-11 President and Fellows of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
US20140272967A1 (en) 2013-03-13 2014-09-18 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
EP2971100A1 (en) 2013-03-13 2016-01-20 Sequenom, Inc. Primers for dna methylation analysis
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
WO2014164716A1 (en) * 2013-03-13 2014-10-09 President And Fellows Of Harvard College Methods of elongating dna
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
US20140287946A1 (en) 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
EP2971159B1 (en) 2013-03-14 2019-05-08 Molecular Loop Biosolutions, LLC Methods for analyzing nucleic acids
EP2971171A4 (en) 2013-03-14 2016-11-02 Abbott Molecular Inc SYSTEMS AND METHODS FOR MULTIPLEX AMPLIFICATION SPECIFIC TO METHYLATION
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
CN105378104A (zh) 2013-03-15 2016-03-02 威拉赛特公司 用于样品分类的方法和组合物
WO2014150910A1 (en) 2013-03-15 2014-09-25 Ibis Biosciences, Inc. Dna sequences to assess contamination in dna sequencing
CN105264366B (zh) 2013-03-15 2019-04-16 生命科技公司 具有一致传感器表面区域的化学传感器
US9890425B2 (en) 2013-03-15 2018-02-13 Abbott Molecular Inc. Systems and methods for detection of genomic copy number changes
WO2014142981A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Enzyme-linked nucleotides
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
CN105051214B (zh) 2013-03-15 2018-12-28 吉纳普赛斯股份有限公司 用于生物分析的系统和方法
GB2528205B (en) 2013-03-15 2020-06-03 Guardant Health Inc Systems and methods to detect rare mutations and copy number variation
US11976329B2 (en) 2013-03-15 2024-05-07 Veracyte, Inc. Methods and systems for detecting usual interstitial pneumonia
US20140274738A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
CA2905517C (en) 2013-03-15 2024-11-12 Lineage Biosciences, Inc. METHODS AND COMPOSITIONS FOR LABELING AND ANALYZING SAMPLES
EP3421613B1 (en) 2013-03-15 2020-08-19 The Board of Trustees of the Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
WO2014149779A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical device with thin conductive element
WO2014149778A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical sensors with consistent sensor surface areas
CN105392897B (zh) * 2013-03-19 2020-03-20 定向基因组学公司 靶序列的富集
EP2986741B1 (en) 2013-04-17 2019-07-24 Agency For Science, Technology And Research Method for generating extended sequence reads
WO2014172152A1 (en) * 2013-04-19 2014-10-23 Siemens Healthcare Diagnostics Inc. Non-contact micro droplet dispenser and method
RU2578009C2 (ru) * 2013-05-08 2016-03-20 Закрытое акционерное общество "ЕВРОГЕН" Способ идентификации нативных пар фрагментов днк или рнк, присутствующих в одних и тех же живых клетках
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
US10160995B2 (en) * 2013-05-13 2018-12-25 Qiagen Waltham, Inc. Analyte enrichment methods and compositions
WO2014190322A2 (en) 2013-05-24 2014-11-27 Quantapore, Inc. Nanopore-based nucleic acid analysis with mixed fret detection
EP3005200A2 (en) 2013-06-03 2016-04-13 Good Start Genetics, Inc. Methods and systems for storing sequence read data
SG10201710030QA (en) 2013-06-04 2018-01-30 Harvard College Rna-guided transcriptional regulation
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
KR101648252B1 (ko) * 2014-01-28 2016-08-16 연세대학교 산학협력단 염기서열 확인 과정에서 분리된 핵산 단편들을 회수하는 방법
US10526640B2 (en) 2013-06-19 2020-01-07 Industry-Academic Cooperation Foundation, Yonsei University Methods for retrieving sequence-verified nucleic acid fragments and apparatuses for amplifying sequence verified nucleic acid fragments
EP4219745B1 (en) 2013-06-25 2025-09-03 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
KR102177225B1 (ko) 2013-07-01 2020-11-10 일루미나, 인코포레이티드 촉매-무함유 표면 작용화 및 중합체 그라프팅
EP3241913B1 (en) 2013-07-03 2019-02-20 Illumina, Inc. System for sequencing by orthogonal synthesis
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
TWI707038B (zh) 2013-08-05 2020-10-11 美商扭轉生物科技有限公司 重新合成之基因庫
CA3091557C (en) 2013-08-08 2022-10-18 Illumina, Inc. Fluidic system for reagent delivery to a flow cell
US20150051117A1 (en) * 2013-08-16 2015-02-19 President And Fellows Of Harvard College Assembly of Nucleic Acid Sequences in Emulsions
EP2840148B1 (en) 2013-08-23 2019-04-03 F. Hoffmann-La Roche AG Methods for nucleic acid amplification
EP2848698A1 (en) 2013-08-26 2015-03-18 F. Hoffmann-La Roche AG System and method for automated nucleic acid amplification
CA2920390A1 (en) 2013-08-30 2015-03-05 Illumina, Inc. Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
EP4567682A3 (en) 2013-08-30 2025-09-03 Personalis, Inc. Methods for genomic analysis
JP6700173B2 (ja) * 2013-09-24 2020-05-27 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア ターゲット検出方法及びシステム
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
WO2015051275A1 (en) 2013-10-03 2015-04-09 Personalis, Inc. Methods for analyzing genotypes
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
WO2015051338A1 (en) 2013-10-04 2015-04-09 Life Technologies Corporation Methods and systems for modeling phasing effects in sequencing using termination chemistry
HUE045954T2 (hu) 2013-10-17 2020-01-28 Illumina Inc Eljárások és készítmények nukleinsavkönyvtárak elõállítására
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
WO2015057565A1 (en) 2013-10-18 2015-04-23 Good Start Genetics, Inc. Methods for assessing a genomic region of a subject
CN103540672B (zh) * 2013-10-29 2015-04-08 中国科学技术大学 一种亲和核酸分子的快速鉴定和分离方法
US10947587B2 (en) * 2013-11-05 2021-03-16 The Regents Of The University Of California Single-cell forensic short tandem repeat typing within microfluidic droplets
EP4130350A1 (en) 2013-11-07 2023-02-08 The Board of Trustees of the Leland Stanford Junior University Cell-free nucleic acids for the analysis of the human microbiome and components thereof
US20160279068A1 (en) 2013-11-08 2016-09-29 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9533240B2 (en) 2013-11-12 2017-01-03 Life Technologies Corporation System and method for emulsion breaking
DK2891722T3 (en) 2013-11-12 2019-01-07 Population Bio Inc METHODS AND COMPOSITIONS FOR DIAGNOSTICING, PROGRAMMING AND TREATMENT OF ENDOMETRIOSIS
EP3068883B1 (en) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
CN104630202A (zh) * 2013-11-13 2015-05-20 北京大学 一种能够减小微量核酸物质整体扩增时产生偏倚的扩增方法
CN110560187B (zh) 2013-11-18 2022-01-11 尹特根埃克斯有限公司 用于样本分析的卡盒和仪器
WO2015085268A1 (en) 2013-12-05 2015-06-11 Centrillion Technology Holdings Corporation Modified surfaces
EP3628747B1 (en) 2013-12-05 2022-10-05 Centrillion Technology Holdings Corporation Fabrication of patterned arrays
EP3077545B1 (en) 2013-12-05 2020-09-16 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
US10100355B2 (en) 2013-12-09 2018-10-16 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
EP3080300B1 (en) * 2013-12-11 2020-09-02 Genapsys Inc. Systems and methods for biological analysis and computation
CA2933387C (en) 2013-12-11 2023-05-02 The Regents Of The University Of California Methods for labeling dna fragments to reconstruct physical linkage and phase
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2015089438A1 (en) 2013-12-13 2015-06-18 Northwestern University Biomarkers for post-traumatic stress states
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015095355A2 (en) 2013-12-17 2015-06-25 The Brigham And Women's Hospital, Inc. Detection of an antibody against a pathogen
SG10201804519RA (en) 2013-12-28 2018-07-30 Guardant Health Inc Methods and systems for detecting genetic variants
WO2015103367A1 (en) 2013-12-31 2015-07-09 Raindance Technologies, Inc. System and method for detection of rna species
WO2015103225A1 (en) 2013-12-31 2015-07-09 Illumina, Inc. Addressable flow cell using patterned electrodes
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
CN106661612A (zh) 2014-01-27 2017-05-10 通用医疗公司 制备用于测序的核酸的方法
US9587268B2 (en) 2014-01-29 2017-03-07 Agilent Technologies Inc. Fast hybridization for next generation sequencing target enrichment
US9387451B2 (en) 2014-02-03 2016-07-12 International Business Machines Corporation Flow cell array and uses thereof
US10384187B2 (en) 2014-02-10 2019-08-20 Gencell Biosystems Ltd Composite liquid cell (CLC) mediated nucleic acid library preparation device, and methods for using the same
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
ES2741740T3 (es) 2014-03-05 2020-02-12 Adaptive Biotechnologies Corp Métodos que usan moléculas sintéticas que contienen segmentos de nucleótidos aleatorios
US11365447B2 (en) 2014-03-13 2022-06-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
JP6607846B2 (ja) * 2014-03-20 2019-11-20 ユニバーサル・バイオ・リサーチ株式会社 核酸増幅自動化装置、及び核酸増幅分析自動化装置
US11060139B2 (en) 2014-03-28 2021-07-13 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US20150284715A1 (en) * 2014-04-07 2015-10-08 Qiagen Gmbh Enrichment Methods
CA2943624A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US9822401B2 (en) 2014-04-18 2017-11-21 Genapsys, Inc. Methods and systems for nucleic acid amplification
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
EP3957749A1 (en) 2014-04-21 2022-02-23 Natera, Inc. Detecting tumour specific mutations in biopsies with whole exome sequencing and in cell-free samples
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
US10208332B2 (en) 2014-05-21 2019-02-19 Integenx Inc. Fluidic cartridge with valve mechanism
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
EP3151733B1 (en) 2014-06-06 2020-04-15 The Regents Of The University Of Michigan Compositions and methods for characterizing and diagnosing periodontal disease
WO2015191877A1 (en) 2014-06-11 2015-12-17 Life Technologies Corporation Systems and methods for substrate enrichment
US20150361481A1 (en) * 2014-06-13 2015-12-17 Life Technologies Corporation Multiplex nucleic acid amplification
US11155809B2 (en) 2014-06-24 2021-10-26 Bio-Rad Laboratories, Inc. Digital PCR barcoding
AU2015279617A1 (en) 2014-06-26 2017-01-12 10X Genomics, Inc. Analysis of nucleic acid sequences
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
EP3161700B1 (en) 2014-06-26 2023-03-29 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
EP3161160B1 (en) 2014-06-26 2021-10-13 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
JP2017521654A (ja) 2014-06-27 2017-08-03 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ ヒトペギウイルス２（ＨＰｇＶ−２）を検出するための組成物および方法
US10697007B2 (en) 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
US12297505B2 (en) 2014-07-14 2025-05-13 Veracyte, Inc. Algorithms for disease diagnostics
SG11201700303UA (en) 2014-07-17 2017-02-27 Celmatix Inc Methods and systems for assessing infertility and related pathologies
EP3191607B1 (en) 2014-07-24 2019-08-21 Abbott Molecular Inc. Compositions for the detection and analysis of mycobacterium tuberculosis
WO2016019360A1 (en) 2014-08-01 2016-02-04 Dovetail Genomics Llc Tagging nucleic acids for sequence assembly
WO2016022696A1 (en) 2014-08-05 2016-02-11 The Trustees Of Columbia University In The City Of New York Method of isolating aptamers for minimal residual disease detection
JP6803327B2 (ja) 2014-08-06 2020-12-23 ニューゲン テクノロジーズ， インコーポレイテッド 標的化されたシークエンシングからのデジタル測定値
GB201413929D0 (en) 2014-08-06 2014-09-17 Geneseque As Method
WO2016025452A1 (en) 2014-08-11 2016-02-18 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acids assays
CN106796212A (zh) 2014-08-12 2017-05-31 新生代吉恩公司 用于基于收集的体液而监测健康的系统和方法
WO2016025818A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
US10435685B2 (en) * 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
US9982250B2 (en) 2014-08-21 2018-05-29 Illumina Cambridge Limited Reversible surface functionalization
US10724096B2 (en) 2014-09-05 2020-07-28 Population Bio, Inc. Methods and compositions for inhibiting and treating neurological conditions
AU2015315351B2 (en) 2014-09-08 2018-06-21 Becton, Dickinson And Company Enhanced platen for pharmaceutical compounding
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
CN105400864B (zh) * 2014-09-12 2020-04-14 深圳华大基因股份有限公司 用于基于血液样品构建测序文库的方法及其在确定胎儿遗传异常中的用途
CN106715713B (zh) * 2014-09-12 2020-11-03 深圳华大智造科技有限公司 试剂盒及其在核酸测序中的用途
JP6483249B2 (ja) * 2014-09-12 2019-03-13 エムジーアイ テック カンパニー リミテッドＭＧＩ Ｔｅｃｈ Ｃｏ．， Ｌｔｄ． 単離されたオリゴヌクレオチドおよび核酸の配列決定におけるその使用
MX378730B (es) 2014-09-15 2025-03-11 Abvitro Llc Secuenciacion de bibliotecas de nucleotidos de alto rendimiento
ES2788692T3 (es) 2014-09-17 2020-10-22 Ibis Biosciences Inc Secuenciación por síntesis usando óptica de lectura por pulsos
JP6802154B2 (ja) 2014-09-18 2020-12-16 イラミーナ インコーポレーテッド 核酸シーケンシングデータを解析するための方法およびシステム
EP3224595A4 (en) 2014-09-24 2018-06-13 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
WO2016057829A1 (en) 2014-10-10 2016-04-14 Quantapore, Inc. Nanopore-based polymer analysis with mutually-quenching fluorescent labels
EP3208343B1 (en) * 2014-10-13 2022-01-05 MGI Tech Co., Ltd. Nucleic acid fragmentation method and sequence combination
WO2016060974A1 (en) 2014-10-13 2016-04-21 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
CN107106983B (zh) 2014-10-22 2021-04-16 尹特根埃克斯有限公司 用于样品制备、处理和分析的系统和方法
CA3001986C (en) 2014-10-22 2023-02-21 The Regents Of The University Of California High definition microdroplet printer
AU2015335616B2 (en) 2014-10-24 2019-09-12 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
EP3715455A1 (en) 2014-10-29 2020-09-30 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
MX2017005267A (es) 2014-10-29 2017-07-26 10X Genomics Inc Metodos y composiciones para la secuenciacion de acidos nucleicos seleccionados como diana.
WO2016070131A1 (en) 2014-10-30 2016-05-06 Personalis, Inc. Methods for using mosaicism in nucleic acids sampled distal to their origin
LT3212684T (lt) 2014-10-31 2020-04-10 Illumina Cambridge Limited Polimerai ir dnr kopolimero dangos
CN107109320B (zh) * 2014-11-04 2021-08-06 凸版印刷株式会社 核酸导入方法、核酸检测方法、生物体成分解析方法及试剂盒、生物体成分定量用阵列器件
US10000799B2 (en) * 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
WO2016073778A2 (en) 2014-11-05 2016-05-12 Nirmidas Biotech, Inc. Metal composites for enhanced imaging
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US20170335396A1 (en) 2014-11-05 2017-11-23 Veracyte, Inc. Systems and methods of diagnosing idiopathic pulmonary fibrosis on transbronchial biopsies using machine learning and high dimensional transcriptional data
WO2016073237A1 (en) 2014-11-05 2016-05-12 Illumina Cambridge Limited Reducing dna damage during sample preparation and sequencing using siderophore chelators
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
US11789906B2 (en) 2014-11-19 2023-10-17 Arc Bio, Llc Systems and methods for genomic manipulations and analysis
CN114606228B (zh) * 2014-11-20 2025-01-07 杭州智灵龙生物科技有限公司 用于核酸扩增的组合物和方法
US10233490B2 (en) 2014-11-21 2019-03-19 Metabiotech Corporation Methods for assembling and reading nucleic acid sequences from mixed populations
CA2968543C (en) 2014-11-25 2024-04-02 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
AU2015357573B2 (en) 2014-12-05 2022-04-07 Foundation Medicine, Inc. Multigene analysis of tumor samples
EP4354131A3 (en) 2014-12-18 2024-06-26 Life Technologies Corporation High data rate integrated circuit with transmitter configuration
KR20170097712A (ko) 2014-12-18 2017-08-28 라이프 테크놀로지스 코포레이션 대형 fet 어레이를 사용한 분석물 측정을 위한 방법과 장치
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
US20160177386A1 (en) 2014-12-18 2016-06-23 Life Technologies Corporation Calibration panels and methods for designing the same
WO2016105199A1 (en) 2014-12-24 2016-06-30 Keygene N.V. Backbone mediated mate pair sequencing
WO2016112073A1 (en) 2015-01-06 2016-07-14 Good Start Genetics, Inc. Screening for structural variants
CN107427808B (zh) 2015-01-12 2020-10-23 10X基因组学有限公司 用于制备核酸测序文库的方法和系统以及用其制备的文库
KR20170106979A (ko) 2015-01-13 2017-09-22 10엑스 제노믹스, 인크. 구조 변이 및 위상 조정 정보를 시각화하기 위한 시스템 및 방법
GB201501012D0 (en) * 2015-01-21 2015-03-04 Base4 Innovation Ltd Improved droplet sequencing apparatus and method
WO2016122582A1 (en) * 2015-01-30 2016-08-04 Hewlett Packard Enterprise Development Lp Relationship preserving projection of digital objects
CN107209120B (zh) 2015-02-02 2020-05-15 株式会社日立高新技术 多色荧光分析装置
WO2016125251A1 (ja) * 2015-02-03 2016-08-11 株式会社日立製作所 単一細胞解析用フローセルデバイス及び単一細胞解析装置
WO2016126871A2 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
EP3256606B1 (en) 2015-02-09 2019-05-22 10X Genomics, Inc. Systems and methods for determining structural variation
JP6755890B2 (ja) * 2015-02-11 2020-09-16 パラゴン ゲノミクス, インク.Paragon Genomics, Inc. 非特異的増幅産物を減少させる為の方法及び組成物
US10421993B2 (en) 2015-02-11 2019-09-24 Paragon Genomics, Inc. Methods and compositions for reducing non-specific amplification products
JP6773687B2 (ja) * 2015-02-17 2020-10-21 エムジーアイ テック カンパニー リミテッドＭＧＩ Ｔｅｃｈ Ｃｏ．， Ｌｔｄ． 制御された鎖置換を用いてのｄｎａ配列決定
WO2016134034A1 (en) 2015-02-17 2016-08-25 Dovetail Genomics Llc Nucleic acid sequence assembly
CN107407685B (zh) 2015-02-20 2021-08-03 宝生物工程（美国)有限公司 快速精确分配、可视化和分析单个细胞的方法
US11047008B2 (en) 2015-02-24 2021-06-29 Adaptive Biotechnologies Corporation Methods for diagnosing infectious disease and determining HLA status using immune repertoire sequencing
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
CN107614096A (zh) 2015-03-13 2018-01-19 哈佛学院院长及董事 使用扩增测定细胞
JP6567685B2 (ja) 2015-03-24 2019-08-28 イラミーナ インコーポレーテッド 生物学的又は化学的な解析用のサンプルを撮像する方法、キャリヤ組立体、及びシステム
WO2016154540A1 (en) 2015-03-26 2016-09-29 Dovetail Genomics Llc Physical linkage preservation in dna storage
WO2016160822A1 (en) * 2015-03-30 2016-10-06 Verily Life Sciences Llc Functionalized nanoparticles, methods and in vivo diagnostic system
US10301660B2 (en) 2015-03-30 2019-05-28 Takara Bio Usa, Inc. Methods and compositions for repair of DNA ends by multiple enzymatic activities
WO2016161273A1 (en) 2015-04-01 2016-10-06 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
JP2018509928A (ja) 2015-04-02 2018-04-12 ザ ジャクソン ラボラトリー 環状化メイトペアライブラリーおよびショットガン配列決定を用いて、ゲノム変異を検出するための方法
DK3901281T4 (da) 2015-04-10 2025-10-20 Illumina Inc Rumligt adskilt, multipleks nukleinsyreanalyse af biologiske prøver
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US11180522B2 (en) 2015-05-08 2021-11-23 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
CA3160383A1 (en) 2015-05-11 2016-11-17 Illumina, Inc. Platform for discovery and analysis of therapeutic agents
WO2016183478A1 (en) 2015-05-14 2016-11-17 Life Technologies Corporation Barcode sequences, and related systems and methods
EP3303584B1 (en) 2015-05-29 2019-10-09 Epicentre Technologies Corporation Methods of analyzing nucleic acids
EP3527672B1 (en) 2015-06-09 2022-10-05 Centrillion Technology Holdings Corporation Oligonucleotide arrays for sequencing nucleic acids
EP3307908B1 (en) 2015-06-09 2019-09-11 Life Technologies Corporation Methods for molecular tagging
US12157910B2 (en) * 2015-07-06 2024-12-03 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
EP3320399A1 (en) 2015-07-07 2018-05-16 Illumina, Inc. Selective surface patterning via nanoimprinting
US10392613B2 (en) 2015-07-14 2019-08-27 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides
WO2017011565A1 (en) 2015-07-14 2017-01-19 Abbott Molecular Inc. Compositions and methods for identifying drug resistant tuberculosis
ES2945607T3 (es) 2015-07-17 2023-07-04 Illumina Inc Láminas de polímero para aplicaciones de secuenciación
US10150994B2 (en) 2015-07-22 2018-12-11 Qiagen Waltham, Inc. Modular flow cells and methods of sequencing
IL294145B2 (en) 2015-07-30 2024-02-01 Illumina Inc Removal of orthogonal blocking of nucleotides
US11929149B2 (en) 2015-08-06 2024-03-12 Arc Bio, Llc Systems and methods for genomic analysis
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
CN108474805A (zh) 2015-08-24 2018-08-31 亿明达股份有限公司 用于生物和化学测定的线路内蓄压器和流量控制系统
EP3344389B1 (en) 2015-09-02 2020-06-10 Illumina Cambridge Limited Method of fixing defects in a hydrophobic surface of a droplet actuator
US9938572B1 (en) 2015-09-08 2018-04-10 Raindance Technologies, Inc. System and method for forming an emulsion
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
HK1258869A1 (zh) 2015-09-18 2019-11-22 Twist Bioscience Corporation 寡核酸变体文库及其合成
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
US10465232B1 (en) 2015-10-08 2019-11-05 Trace Genomics, Inc. Methods for quantifying efficiency of nucleic acid extraction and detection
CA3001679A1 (en) 2015-10-13 2017-04-20 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
CN108368542B (zh) 2015-10-19 2022-04-08 多弗泰尔基因组学有限责任公司 用于基因组组装、单元型定相以及独立于靶标的核酸检测的方法
CN108474022A (zh) 2015-11-03 2018-08-31 哈佛学院董事及会员团体 用于包含三维核酸的基质容积成像的设备和方法
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
US9895673B2 (en) 2015-12-01 2018-02-20 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
KR20240161696A (ko) 2015-12-04 2024-11-12 10엑스 제노믹스, 인크. 핵산 분석을 위한 방법 및 조성물
WO2017096110A1 (en) * 2015-12-04 2017-06-08 Siemens Healthcare Diagnostics Inc. Method and device for optimizing the process of identification of pathogens
CN108431223A (zh) 2016-01-08 2018-08-21 生物辐射实验室股份有限公司 每液滴分辨率下的多个珠
AU2017207259B2 (en) 2016-01-11 2019-06-27 Illumina Singapore Pte Ltd Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module
US10612089B2 (en) * 2016-01-12 2020-04-07 Bio-Rad Laboratories, Inc. Synthesizing barcoding sequences utilizing phase-shift blocks and uses thereof
US20190169602A1 (en) 2016-01-12 2019-06-06 Seqwell, Inc. Compositions and methods for sequencing nucleic acids
KR102641264B1 (ko) 2016-01-22 2024-02-26 퍼듀 리서치 파운데이션 하전된 질량 표지 시스템
JP6685138B2 (ja) 2016-01-27 2020-04-22 シスメックス株式会社 核酸増幅の精度管理方法、精度管理用試薬およびその試薬キット
EP3848457B1 (en) 2016-02-08 2025-08-13 Rgene, Inc. Multiple ligase compositions, systems, and methods
CN108779491B (zh) 2016-02-11 2021-03-09 10X基因组学有限公司 用于全基因组序列数据的从头组装的系统、方法和介质
JP2017143783A (ja) * 2016-02-17 2017-08-24 国立大学法人 筑波大学 並列反応用懸濁液、並列反応方法、スクリーニング方法および検査方法
EP3420108A4 (en) 2016-02-23 2019-11-06 Dovetail Genomics LLC GENERATION OF LIVE PHASES FOR THE GENERATION ARRANGEMENT AND HAPLOTYPE PHASE RULES
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
WO2017160788A2 (en) 2016-03-14 2017-09-21 RGENE, Inc. HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES
EP4574992A3 (en) 2016-03-22 2025-07-16 Myriad Women's Health, Inc. Combinatorial dna screening
CN116987777A (zh) 2016-03-25 2023-11-03 凯锐思公司 合成核酸掺入物
DK3377226T3 (da) 2016-03-28 2021-04-26 Illumina Inc Mikrorækker i flere niveauer
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
WO2017180909A1 (en) 2016-04-13 2017-10-19 Nextgen Jane, Inc. Sample collection and preservation devices, systems and methods
WO2017180871A1 (en) 2016-04-13 2017-10-19 Purdue Research Foundation Systems and methods for isolating a target in an ion trap
ITUA20162640A1 (it) * 2016-04-15 2017-10-15 Menarini Silicon Biosystems Spa Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo
JP7280044B2 (ja) 2016-04-15 2023-05-23 ナテラ， インコーポレイテッド 肺癌の検出方法
CA3022290A1 (en) 2016-04-25 2017-11-02 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
CN105821482B (zh) * 2016-04-29 2018-04-10 李星军 一种生化微反应体系、高通量测序的建库仪及应用
US10077459B2 (en) 2016-05-04 2018-09-18 General Electric Company Cell-free protein expression using rolling circle amplification product
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
KR102412442B1 (ko) 2016-05-13 2022-06-22 더브테일 제노믹스 엘엘씨 보존된 샘플로부터의 장범위 링키지 정보의 회수
AU2017267653B2 (en) 2016-05-18 2021-05-13 Illumina, Inc. Self assembled patterning using patterned Hydrophobic surfaces
US11299783B2 (en) 2016-05-27 2022-04-12 Personalis, Inc. Methods and systems for genetic analysis
WO2017214417A1 (en) * 2016-06-10 2017-12-14 Takara Bio Usa, Inc. Methods and compositions employing blocked primers
US11268117B2 (en) 2016-06-10 2022-03-08 Life Technologies Corporation Methods and compositions for nucleic acid amplification
JP2019528675A (ja) * 2016-06-14 2019-10-17 ベース４ イノベーション リミテッド ポリヌクレオチド分離法
US11091795B2 (en) 2016-07-11 2021-08-17 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
CN109790575A (zh) 2016-07-20 2019-05-21 吉纳普赛斯股份有限公司 用于核酸测序的系统和方法
WO2018017892A1 (en) 2016-07-21 2018-01-25 Takara Bio Usa, Inc. Multi-z imaging and dispensing with multi-well devices
WO2018031691A1 (en) 2016-08-10 2018-02-15 The Regents Of The University Of California Combined multiple-displacement amplification and pcr in an emulsion microdroplet
AU2017315252B2 (en) 2016-08-22 2023-03-16 Biolumic Limited System, device and methods of seed treatment
SG11201901563UA (en) 2016-08-22 2019-03-28 Twist Bioscience Corp De novo synthesized nucleic acid libraries
US11414708B2 (en) 2016-08-24 2022-08-16 Decipher Biosciences, Inc. Use of genomic signatures to predict responsiveness of patients with prostate cancer to post-operative radiation therapy
MX2019002376A (es) * 2016-08-31 2019-06-20 Harvard College Metodos de amplificacion digital del genoma completo.
CN109983125B (zh) 2016-08-31 2024-06-04 哈佛学院董事及会员团体 生成用于通过荧光原位测序检测的核酸序列文库的方法
CN118853848A (zh) 2016-08-31 2024-10-29 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
WO2018045162A1 (en) 2016-09-01 2018-03-08 Biogen Ma Inc. Biomarkers predictive of primary progressive multiple sclerosis and uses thereof
US20180073063A1 (en) * 2016-09-09 2018-03-15 Tl Biolabs Corp. Reusable microarray compositions and methods
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US11572555B2 (en) 2016-09-27 2023-02-07 Psomagen, Inc. Method and system for CRISPR-based library preparation and sequencing
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
CN111781139B (zh) 2016-10-14 2023-09-12 亿明达股份有限公司 夹盒组件
US10190155B2 (en) 2016-10-14 2019-01-29 Nugen Technologies, Inc. Molecular tag attachment and transfer
US11725232B2 (en) 2016-10-31 2023-08-15 The Hong Kong University Of Science And Technology Compositions, methods and kits for detection of genetic variants for alzheimer's disease
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10255990B2 (en) 2016-11-11 2019-04-09 uBiome, Inc. Method and system for fragment assembly and sequence identification
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
WO2018111728A1 (en) * 2016-12-12 2018-06-21 Slipchip Corporation Capsule containment of dried reagents
AU2017378492B2 (en) 2016-12-16 2022-06-16 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
EP3555290B1 (en) 2016-12-19 2022-11-02 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
EP3559269B1 (en) * 2016-12-20 2020-09-16 F. Hoffmann-La Roche AG Single stranded circular dna libraries for circular consensus sequencing
CA3047328A1 (en) 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11466318B2 (en) 2016-12-27 2022-10-11 Egi Tech (Shen Zhen) Co., Limited Single fluorescent dye-based sequencing method
RS63419B2 (sr) * 2017-01-04 2025-10-31 Mgi Tech Co Ltd Sekvenciranje nukleinske kiseline upotrebom afinitetnih reagenasa
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
US10100358B2 (en) 2017-01-10 2018-10-16 Paragon Genomics, Inc. Methods and compositions for reducing redundant molecular barcodes created in primer extension reactions
CA3050984A1 (en) 2017-01-20 2018-07-26 Decipher Biosciences, Inc. Molecular subtyping, prognosis, and treatment of bladder cancer
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
SG11201906442TA (en) 2017-02-01 2019-08-27 Illumina Inc System and method with fiducials responding to multiple excitation frequencies
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
US10240205B2 (en) 2017-02-03 2019-03-26 Population Bio, Inc. Methods for assessing risk of developing a viral disease using a genetic test
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US10894976B2 (en) 2017-02-21 2021-01-19 Natera, Inc. Compositions, methods, and kits for isolating nucleic acids
US11550939B2 (en) 2017-02-22 2023-01-10 Twist Bioscience Corporation Nucleic acid based data storage using enzymatic bioencryption
GB201703049D0 (en) * 2017-02-24 2017-04-12 Univ I Tromsø - Norges Arktiske Univ Single-strand binding protein
EP4218738B1 (en) * 2017-02-24 2024-10-16 The Regents of The University of California Particle-drop structures and methods for making and using the same
JP6931540B2 (ja) * 2017-02-27 2021-09-08 シスメックス株式会社 検体処理チップを用いた送液方法、検体処理チップの送液装置
WO2018165600A1 (en) 2017-03-09 2018-09-13 Genomedx Biosciences, Inc. Subtyping prostate cancer to predict response to hormone therapy
EP3595674A4 (en) 2017-03-15 2020-12-16 Twist Bioscience Corporation BANKS OF VARIANTS OF IMMUNOLOGICAL SYNAPSE AND THEIR SYNTHESIS
CN110446787A (zh) 2017-03-24 2019-11-12 生物辐射实验室股份有限公司 通用发夹引物
EP3607087A4 (en) 2017-04-04 2020-12-30 Omniome, Inc. FLUIDIC APPARATUS AND USEFUL METHODS FOR CHEMICAL AND BIOLOGICAL REACTIONS
FI3607065T3 (fi) * 2017-04-06 2023-06-16 Qing Wang Menetelmä ja tarvikesarja nukleiinihappokirjaston rakentamista varten
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
WO2018191563A1 (en) 2017-04-12 2018-10-18 Karius, Inc. Sample preparation methods, systems and compositions
CN109789167A (zh) 2017-04-14 2019-05-21 哈佛学院董事及会员团体 用于产生细胞衍生的微丝网络的方法
US20210371918A1 (en) 2017-04-18 2021-12-02 Dovetail Genomics, Llc Nucleic acid characteristics as guides for sequence assembly
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
JP2018183097A (ja) * 2017-04-26 2018-11-22 株式会社エンプラス 細胞由来の解析用液滴の単離方法、および細胞の解析方法
CA3062716A1 (en) 2017-05-12 2018-11-15 Decipher Biosciences, Inc. Genetic signatures to predict prostate cancer metastasis and identify tumor agressiveness
CN106957842B (zh) * 2017-05-15 2020-05-22 青岛安德贝生命科技有限公司 Bac克隆dna的提取方法
WO2018213774A1 (en) 2017-05-19 2018-11-22 10X Genomics, Inc. Systems and methods for analyzing datasets
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN110997934B (zh) * 2017-05-31 2025-02-28 生捷科技控股公司 具有电子检测系统的寡核苷酸探针阵列
CN110997711A (zh) 2017-06-08 2020-04-10 布里格姆妇女医院 用于鉴定表位的方法和组合物
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11001885B2 (en) * 2017-06-13 2021-05-11 Personal Genomics Taiwan, Inc. Apparatus for single molecular sequencing and method of sequencing nucleic acid molecules
EP3642363B1 (en) 2017-06-20 2025-12-24 Bio-Rad Laboratories, Inc. Mda using bead oligonucleotide
US12040053B2 (en) 2017-06-21 2024-07-16 Bluedot, Llc Methods for generating sequencer-specific nucleic acid barcodes that reduce demultiplexing errors
US11217329B1 (en) 2017-06-23 2022-01-04 Veracyte, Inc. Methods and systems for determining biological sample integrity
MX2019015877A (es) 2017-06-29 2020-08-06 Biolumic Ltd Método para mejorar el rendimiento o la calidad de los cultivos.
CN111133135B (zh) * 2017-07-17 2024-04-16 希昆斯生物科学公司 用于高通量测序的快速文库构建
CA3072061A1 (en) 2017-08-04 2019-02-07 The Regents Of The University Of Michigan Use of immune cell-specific gene expression for prognosis of prostate cancer and prediction of responsiveness to radiation therapy
IL319255A (en) 2017-08-07 2025-04-01 Univ Johns Hopkins Methods and materials for cancer assessment and treatment
CN111182972B (zh) 2017-08-15 2022-03-08 欧姆尼奥姆股份有限公司 用于检测化学和生物分析物的扫描装置和方法
WO2019038594A2 (en) 2017-08-21 2019-02-28 Biolumic Limited TRANSGENIC PLANTS WITH HIGH GROWTH AND HIGH RUSTICITY
EP3673064A4 (en) 2017-08-24 2021-05-26 Takara Bio USA, Inc. METHOD OF PRODUCING NUCLEIC ACIDS USING STIMULUS MODIFIED OLIGONUCLEOTIDES
AU2018327337A1 (en) * 2017-09-07 2020-04-30 Coopersurgical, Inc. Systems and methods for non-invasive preimplantation genetic diagnosis
GB2581620A (en) 2017-09-11 2020-08-26 Twist Bioscience Corp GPCR binding proteins and synthesis thereof
RU2020113600A (ru) 2017-09-20 2021-10-20 Ридженерон Фармасьютикалз, Инк. Способы иммунотерапии пациентов, опухоли которых характеризуются высокой нагрузкой пассажирскими генными мутациями
MX2020003113A (es) 2017-09-21 2020-09-07 Genapsys Inc Sistemas y metodos para secuenciar acido nucleico.
WO2019060716A1 (en) 2017-09-25 2019-03-28 Freenome Holdings, Inc. SAMPLE EXTRACTION METHODS AND SYSTEMS
ES2998086T3 (en) * 2017-09-29 2025-02-19 Univ California Method of generating monodisperse emulsions
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US11598780B2 (en) 2017-10-06 2023-03-07 The University Of Chicago Engineering lymphocytes with specific alpha and beta chains on their t-cell receptor
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
CA3079411C (en) 2017-10-19 2023-12-05 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
CA3079613A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019084165A1 (en) 2017-10-27 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR SAMPLE PREPARATION AND ANALYSIS
CN118186055A (zh) 2017-11-02 2024-06-14 生物辐射实验室股份有限公司 基于转座酶的基因组分析
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
AU2018372906B2 (en) 2017-11-22 2025-04-03 The Regents Of The University Of Michigan Compositions and methods for treating cancer
SG11202004649SA (en) 2017-11-29 2020-06-29 Xgenomes Corp Sequencing of nucleic acids by emergence
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
US11427867B2 (en) 2017-11-29 2022-08-30 Xgenomes Corp. Sequencing by emergence
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019118355A1 (en) 2017-12-12 2019-06-20 10X Genomics, Inc. Systems and methods for single cell processing
WO2019118926A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
CN111712579B (zh) 2017-12-22 2024-10-15 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
JP2021509024A (ja) 2018-01-02 2021-03-18 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン 多数の液滴の捕捉
GB2585506A (en) 2018-01-04 2021-01-13 Twist Bioscience Corp DNA-based digital information storage
US20190237163A1 (en) 2018-01-12 2019-08-01 Life Technologies Corporation Methods for flow space quality score prediction by neural networks
AU2019214956B2 (en) 2018-01-31 2025-06-26 Dovetail Genomics, Llc Sample prep for DNA linkage recovery
CN111699253B (zh) 2018-01-31 2025-01-14 生物辐射实验室股份有限公司 用于解卷积分区条码的方法和组合物
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US12398389B2 (en) 2018-02-15 2025-08-26 Natera, Inc. Methods for isolating nucleic acids with size selection
CN110184325A (zh) 2018-02-22 2019-08-30 张家港万众一芯生物科技有限公司 基于微孔阵列芯片的单分子文库pcr扩增的基因测序方法
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
CA3133334A1 (en) * 2018-03-13 2019-09-19 Inanc ORTAC Methods for single molecule sequencing
CN112166199B (zh) 2018-04-02 2025-04-29 埃努梅拉分子股份有限公司 用于对核酸分子进行计数的方法、系统和组合物
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
EP3553182A1 (en) 2018-04-11 2019-10-16 Université de Bourgogne Detection method of somatic genetic anomalies, combination of capture probes and kit of detection
US11746151B2 (en) 2018-04-13 2023-09-05 The Regents Of The University Of Michigan Compositions and methods for treating cancer
US12024738B2 (en) 2018-04-14 2024-07-02 Natera, Inc. Methods for cancer detection and monitoring
US20190338352A1 (en) 2018-04-19 2019-11-07 Omniome, Inc. Accuracy of base calls in nucleic acid sequencing methods
EP4234718A3 (en) 2018-04-26 2023-11-29 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
US12235273B2 (en) 2018-05-04 2025-02-25 Abbott Laboratories HBV diagnostic, prognostic, and therapeutic methods and products
CN108588200A (zh) * 2018-05-06 2018-09-28 湖南大地同年生物科技有限公司 一种R-Loop高通量测序文库构建方法
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
US12234377B2 (en) 2018-05-18 2025-02-25 The University Of North Carolina At Chapel Hill Compositions, devices, and methods for improving a surface property of a substrate
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
US11814750B2 (en) 2018-05-31 2023-11-14 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
US10801064B2 (en) 2018-05-31 2020-10-13 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
AU2019276719B2 (en) 2018-05-31 2025-11-13 Pacific Biosciences Of California, Inc. Increased signal to noise in nucleic acid sequencing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
WO2019241250A1 (en) 2018-06-11 2019-12-19 Foundation Medicine, Inc. Compositions and methods for evaluating genomic alterations
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CN112567048A (zh) 2018-07-24 2021-03-26 欧姆尼欧美公司 三元复合物种类的连续形成
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
WO2020028194A1 (en) 2018-07-30 2020-02-06 Readcoor, Inc. Methods and systems for sample processing or analysis
EP3830289A1 (en) 2018-08-03 2021-06-09 10X Genomics, Inc. Methods and systems to minimize barcode exchange
FI4417708T3 (fi) 2018-08-08 2025-12-04 Pml Screening Llc Menetelmiä virustaudin kehittymisen riskin arvioimiseksi geneettisen testin avulla
CN118406747A (zh) 2018-08-20 2024-07-30 生物辐射实验室股份有限公司 通过分区中条码珠共定位生成核苷酸序列
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020069424A1 (en) 2018-09-28 2020-04-02 Centrillion Technologies, Inc. Disulfide-linked reversible terminators
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
CN117778535A (zh) 2018-10-25 2024-03-29 Illumina公司 使用索引和条形码识别阵列上的配体的方法和组合物
WO2020102513A1 (en) 2018-11-14 2020-05-22 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for characterizing and treating cancer
WO2020101795A1 (en) 2018-11-15 2020-05-22 Omniome, Inc. Electronic detection of nucleic acid structure
EP3887545A4 (en) * 2018-11-29 2022-08-24 XGenomes Corp. COALESCENCE SEQUENCING
WO2020117653A1 (en) 2018-12-04 2020-06-11 Omniome, Inc. Mixed-phase fluids for nucleic acid sequencing and other analytical assays
JP2022509850A (ja) 2018-12-07 2022-01-24 オクタント，インク． タンパク質間相互作用スクリーニングのためのシステム
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020123320A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Imaging system hardware
AU2019398307A1 (en) 2018-12-13 2021-06-10 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
BR112020026667A2 (pt) * 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
US11041199B2 (en) 2018-12-20 2021-06-22 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11358137B2 (en) 2018-12-26 2022-06-14 Industrial Technology Research Institute Tubular structure for producing droplets and method for producing droplets
EP3902816A4 (en) 2018-12-26 2022-09-14 Twist Bioscience Corporation HIGHLY ACCURATE POLYNUCLEOTIDE DE NOVO SYNTHESIS
CN109738469A (zh) * 2018-12-29 2019-05-10 赛纳生物科技(北京)有限公司 一种fop表面微坑镀膜的致密性检测方法
US20220081714A1 (en) 2019-01-04 2022-03-17 Northwestern University Storing temporal data into dna
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020146740A1 (en) 2019-01-10 2020-07-16 Iovance Biotherapeutics, Inc. System and methods for monitoring adoptive cell therapy clonality and persistence
WO2020167656A1 (en) * 2019-02-11 2020-08-20 Ultima Genomics, Inc. Methods for nucleic acid analysis
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
EP3924513B1 (en) 2019-02-14 2023-04-12 Pacific Biosciences of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
CN113613787B (zh) 2019-02-20 2023-06-13 加利福尼亚太平洋生物科学股份有限公司 用于检测化学和生物分析物的扫描装置和方法
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
AU2020227802A1 (en) 2019-02-26 2021-10-14 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
JP2022521551A (ja) 2019-02-26 2022-04-08 ツイスト バイオサイエンス コーポレーション Ｇｌｐ１受容体の変異体核酸ライブラリ
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
EP3937780A4 (en) 2019-03-14 2022-12-07 InSilixa, Inc. METHODS AND SYSTEMS FOR TIMED FLUORESCENCE-BASED DETECTION
WO2020191389A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
EP3947718A4 (en) 2019-04-02 2022-12-21 Enumera Molecular, Inc. METHODS, SYSTEMS AND COMPOSITIONS FOR COUNTING NUCLEIC ACID MOLECULES
US11989216B2 (en) 2019-04-09 2024-05-21 University Of Washington Systems and methods for providing similarity-based retrieval of information stored in DNA
CN109853047A (zh) * 2019-04-10 2019-06-07 翌圣生物科技（上海）有限公司 一种基因组dna测序文库快速构建方法及配套试剂盒
BR112021006173A2 (pt) 2019-04-29 2021-11-16 Illumina Inc Métodos para a identificação e análise de micro-organismos viáveis e/ou proliferativos e para determinação da eficácia de um agente antimicrobiano na modulação do crescimento e da proliferação de micro-organismos em uma amostra
US20210017596A1 (en) * 2019-05-08 2021-01-21 Qiagen Sciences, Llc Sequential sequencing methods and compositions
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
AU2020280104A1 (en) 2019-05-22 2022-01-20 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
US20220213546A1 (en) * 2019-05-22 2022-07-07 Oxford Nanopore Technologies Plc Protocol for detecting interactions within one or more dna molecules within a cell
EP3976795A1 (en) 2019-05-28 2022-04-06 Octant, Inc. Transcriptional relay system
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2020247263A1 (en) 2019-06-06 2020-12-10 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
WO2020252186A1 (en) 2019-06-11 2020-12-17 Omniome, Inc. Calibrated focus sensing
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2020264185A1 (en) 2019-06-27 2020-12-30 Dovetail Genomics, Llc Methods and compositions for proximity ligation
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
US12496305B2 (en) 2019-07-30 2025-12-16 The Cleveland Clinic Foundation Detection for determining corticosteroid responsiveness
CN110499361B (zh) * 2019-07-31 2022-11-25 齐鲁工业大学 一种末端碱基流式荧光测序微球的制备方法及应用
EP4020484A4 (en) 2019-08-19 2023-08-30 Green Cross Genome Corporation METHOD FOR DETECTING A CHROMOSOME ABNORMALITY USING INFORMATION CONCERNING THE DISTANCE BETWEEN NUCLEIC ACID FRAGMENTS
JP7675700B2 (ja) * 2019-08-21 2025-05-13 ライフ テクノロジーズ コーポレーション シーケンシングのためのシステムおよび方法
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
US11180520B2 (en) 2019-09-10 2021-11-23 Omniome, Inc. Reversible modifications of nucleotides
WO2021055864A1 (en) 2019-09-20 2021-03-25 Illumina, Inc. Methods and compositions for identifying ligands on arrays using indexes and barcodes
EP4034564A4 (en) 2019-09-23 2023-12-13 Twist Bioscience Corporation VARIANT NUCLEIC ACID LIBRARIES FOR SINGLE-DOMAIN ANTIBODIES
US12091777B2 (en) 2019-09-23 2024-09-17 Twist Bioscience Corporation Variant nucleic acid libraries for CRTH2
CA3157359A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
CN118186063A (zh) 2019-10-18 2024-06-14 加利福尼亚太平洋生物科学股份有限公司 用于对核酸加帽的方法和组合物
CN114945987A (zh) 2019-11-05 2022-08-26 佩索纳里斯公司 从单一样品预估肿瘤纯度
CA3151629A1 (en) 2019-11-07 2021-05-14 Laura E. BENJAMIN Classification of tumor microenvironments
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
CN110951852B (zh) * 2019-11-25 2022-11-25 齐鲁工业大学 单碱基连续延伸流式靶向测序法
US20230028790A1 (en) 2019-11-29 2023-01-26 GC Genome Corporation Artificial intelligence-based chromosomal abnormality detection method
EP3839509A1 (en) * 2019-12-16 2021-06-23 Blink AG A library of prefabricated microparticles and precursors thereof
CN115038794A (zh) 2019-12-23 2022-09-09 10X基因组学有限公司 在基于分区的测定中使用固定生物样品的组合物和方法
EP4667585A2 (en) 2019-12-23 2025-12-24 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
AU2021208466A1 (en) 2020-01-13 2022-08-11 Fluent Biosciences Inc. Single cell sequencing
US11827936B2 (en) 2020-01-13 2023-11-28 Fluent Biosciences Inc. Methods and systems for single cell gene profiling
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
CA3167729A1 (en) 2020-01-13 2021-07-22 Fluent Biosciences Inc. Emulsion based drug screening
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US20230054204A1 (en) 2020-02-04 2023-02-23 Pacific Biosciences Of California, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
CN115349128A (zh) 2020-02-13 2022-11-15 齐默尔根公司 宏基因组文库和天然产物发现平台
EP4104179A1 (en) 2020-02-13 2022-12-21 10X Genomics, Inc. Systems and methods for joint interactive visualization of gene expression and dna chromatin accessibility
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
IL295297A (en) 2020-02-14 2022-10-01 Univ Johns Hopkins Methods and materials for assessing nucleic acids
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
CA3175931A1 (en) 2020-03-16 2021-09-23 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
EP4127058A4 (en) 2020-03-24 2024-04-24 Fluent Biosciences Inc. VIRAL DETECTION USING MATRIX EMULSIFICATION
CN111455469B (zh) * 2020-04-07 2023-08-18 深圳易倍科华生物科技有限公司 一种单链快速建库方法及建库仪器
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
EP4146822A1 (en) 2020-05-05 2023-03-15 Pacific Biosciences of California, Inc. Compositions and methods for modifying polymerase-nucleic acid complexes
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
WO2021236625A1 (en) 2020-05-19 2021-11-25 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
US10941453B1 (en) 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
CA3179313A1 (en) 2020-05-27 2021-12-02 Gillian Sue Dite Methods of assessing risk of developing a severe response to coronavirus infection
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
EP4600376A3 (en) 2020-06-02 2025-10-22 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4162075A4 (en) 2020-06-08 2024-06-26 The Broad Institute, Inc. Single cell combinatorial indexing from amplified nucleic acids
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
EP4165207B1 (en) 2020-06-10 2024-09-25 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
CN115956128A (zh) 2020-07-08 2023-04-11 豪夫迈·罗氏有限公司 在下一代测序文库的靶捕获过程中使用毒性引物对非靶文库分子进行靶向消耗
CA3189597A1 (en) 2020-07-15 2022-01-20 Fluent Biosciences Inc. Tiered ligation oligos
EP4185301A4 (en) 2020-07-24 2025-05-07 The Regents Of The University Of Michigan Compositions and methods for detecting and treating high grade subtypes of uterine cancer
EP4121557A4 (en) 2020-08-06 2024-05-01 Singular Genomics Systems, Inc. Spatial sequencing
US11492662B2 (en) 2020-08-06 2022-11-08 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
EP4491742A3 (en) 2020-09-18 2025-05-21 10x Genomics, Inc. Sample handling apparatus and image registration methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
IL301694A (en) 2020-10-15 2023-05-01 Univ Leland Stanford Junior Detection and analysis of structural variations in genomes
EP4240868A4 (en) * 2020-11-03 2024-11-13 Illumina, Inc. METHODS AND SYSTEMS FOR DETECTING PATHOGENIC MICROBES IN A PATIENT
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11959074B2 (en) * 2020-11-14 2024-04-16 Life Technologies Corporation System and method for automated repeat sequencing
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
KR102795708B1 (ko) 2020-11-27 2025-04-16 주식회사 지씨지놈 인공지능 기반 암 진단 및 암 종 예측방법
EP4257666A4 (en) * 2020-12-04 2024-11-20 Yamaguchi University Nucleic acid amplification method and thermal cycler
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
CA3204784A1 (en) 2021-01-13 2022-07-21 Alex Nemiroski Surface structuring with colloidal assembly
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
WO2022174054A1 (en) 2021-02-13 2022-08-18 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
EP4294571B8 (en) 2021-02-19 2024-07-10 10X Genomics, Inc. Method of using a modular assay support device
EP4298244A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
EP4301870B1 (en) 2021-03-18 2025-01-15 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
US20220333178A1 (en) 2021-03-22 2022-10-20 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
WO2022208171A1 (en) 2021-03-31 2022-10-06 UCL Business Ltd. Methods for analyte detection
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
US12176070B2 (en) * 2021-04-19 2024-12-24 University Of Utah Research Foundation Systems and methods for facilitating rapid genome sequence analysis
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2022240555A1 (en) 2021-05-14 2022-11-17 Pluton Biosciences, Inc. Chryseobacterium insect inhibitory microbial compositions and methods of making and using
KR20220160806A (ko) 2021-05-28 2022-12-06 주식회사 지씨지놈 세포유리 핵산단편 말단 서열 모티프 빈도 및 크기를 이용한 암 진단 및 암 종 예측방법
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
EP4355476A1 (en) 2021-06-15 2024-04-24 Illumina, Inc. Hydrogel-free surface functionalization for sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11535892B1 (en) 2021-06-17 2022-12-27 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
CN113174388B (zh) * 2021-06-28 2021-09-10 中国农业大学 一种功能核酸纳米杆与功能核酸纳米花的制备及形貌转换方法
WO2023003757A1 (en) 2021-07-19 2023-01-26 Illumina Software, Inc. Intensity extraction with interpolation and adaptation for base calling
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
CA3226451A1 (en) 2021-07-21 2023-01-26 Sam Reed Method and system comprising a cartridge for sequencing target polynucleotides
GB202110479D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
GB202110485D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
EP4373958A1 (en) 2021-07-23 2024-05-29 Illumina, Inc. Methods for preparing substrate surface for dna sequencing
CA3229536A1 (en) * 2021-08-20 2023-02-23 Florian OBERSTRASS Systems and methods for sample preparation for sequencing
EP4344443A1 (en) 2021-08-31 2024-04-03 Illumina, Inc. Flow cell with enhanced well imaging resolution
EP4196605B1 (en) 2021-09-01 2024-12-04 10X Genomics, Inc. Methods for blocking a capture probe on a spatial array
WO2023049215A1 (en) 2021-09-22 2023-03-30 Illumina, Inc. Compressed state-based base calling
WO2023059654A1 (en) 2021-10-05 2023-04-13 Personalis, Inc. Customized assays for personalized cancer monitoring
WO2023069927A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
WO2023070567A1 (zh) * 2021-10-29 2023-05-04 京东方科技集团股份有限公司 检测芯片及其制备方法
EP4426489A2 (en) * 2021-11-03 2024-09-11 Abbott Laboratories Systems and methods for sample analysis
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023108014A1 (en) 2021-12-07 2023-06-15 Luminex Corporation Methods and compositions for nucleic acid analysis
WO2023114190A1 (en) * 2021-12-13 2023-06-22 Cz Biohub Sf, Inc. Single-cell epigenomic profiling using fluidics and hydrogels
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
AU2022425440A1 (en) 2021-12-29 2024-07-11 Illumina, Inc. Automatically switching variant analysis model versions for genomic analysis applications
EP4463568A1 (en) 2022-01-13 2024-11-20 Oslo Universitetssykehus HF Prostate cancer markers and uses thereof
WO2023141154A1 (en) 2022-01-20 2023-07-27 Illumina Cambridge Limited Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
WO2023152568A2 (en) 2022-02-10 2023-08-17 Oslo Universitetssykehus Hf Compositions and methods for characterizing lung cancer
CA3223653A1 (en) * 2022-03-15 2023-09-21 Aathavan KARUNAKARAN Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection
US20230343414A1 (en) 2022-03-25 2023-10-26 Illumina, Inc. Sequence-to-sequence base calling
CN114592023B (zh) * 2022-03-31 2023-03-24 杭州优玛达生物科技有限公司 一种细胞裂解自组装多肽复合物、自组装方法、自组装多肽制剂及应用
EP4253550A1 (en) 2022-04-01 2023-10-04 GenCC GmbH 6 Co. KG Method for the manufacture of a viral system, a vector system or any transport system for cancer-specific crispr complexes
US12091715B2 (en) 2022-04-21 2024-09-17 Paragon Genomics, Inc. Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
US20230392201A1 (en) 2022-06-06 2023-12-07 Element Biosciences, Inc. Methods for assembling and reading nucleic acid sequences from mixed populations
CN119317964A (zh) 2022-06-09 2025-01-14 因美纳有限公司 碱基检出对流通池倾斜的依赖
WO2024050450A1 (en) 2022-08-31 2024-03-07 Gigamune, Inc. Engineered enveloped vectors and methods of use thereof
US20240133892A1 (en) 2022-09-27 2024-04-25 Nautilus Subsidiary, Inc. Polypeptide capture, in situ fragmentation and identification
US12373579B2 (en) 2022-11-09 2025-07-29 Bank Of America Corporation Data processing and storage using quantum and DNA computing
CN121127582A (zh) 2022-12-05 2025-12-12 特韦斯特生物科学公司 用于文库制备的酶
WO2024129672A1 (en) 2022-12-12 2024-06-20 The Broad Institute, Inc. Trafficked rnas for assessment of cell-cell connectivity and neuroanatomy
WO2024129969A1 (en) 2022-12-14 2024-06-20 Illumina, Inc. Systems and methods for capture and enrichment of clustered beads on flow cell substrates
WO2024220475A1 (en) 2023-04-21 2024-10-24 Twist Bioscience Corporation Polymerase variants
WO2024249961A1 (en) 2023-06-01 2024-12-05 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues
US20250140346A1 (en) 2023-10-30 2025-05-01 Myriad Women's Health, Inc. Sensitivity of tumor-informed minimal residual disease panels
US20250140343A1 (en) 2023-10-30 2025-05-01 Myriad Women's Health, Inc. Methods for improving minimal residual disease assays
US20250137038A1 (en) 2023-10-30 2025-05-01 Myriad Women's Health, Inc. Sensitivity and estimation of tumor-informed minimal residual disease panels
US20250137061A1 (en) 2023-10-30 2025-05-01 Myriad Women's Health, Inc. Methods for detection and quantitation of circulating tumor dna
US20250137064A1 (en) 2023-10-30 2025-05-01 Myriad Women's Health, Inc. Use of multi-nucleotide and structural variants for improved sensitivity and specificity of circulating tumor dna assays
WO2025104619A1 (en) 2023-11-13 2025-05-22 Oslo Universitetssykehus Hf Breast cancer markers and uses thereof
CN118086457B (zh) * 2024-02-22 2025-03-11 纳昂达(南京)生物科技有限公司 Dna文库的构建方法及应用
US20250320550A1 (en) 2024-04-12 2025-10-16 Robert Bosch Gmbh Sequencing by synthesis using electroactively labeled 3-oh-modified nucleotides
WO2025240460A2 (en) 2024-05-14 2025-11-20 Roche Molecular Systems, Inc. Assay for detection of mutations conferring resistance to treatment with an immunotherapeutic agent
WO2025240905A1 (en) 2024-05-17 2025-11-20 The Broad Institute, Inc. Imaging-free high-resolution spatial macromolecule abundance reconstruction

Family Cites Families (274)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4175054A (en) 1976-11-11 1979-11-20 Petrolite Corporation Use of hydrocarbon polymers in demulsification
US5821058A (en) 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US5171534A (en) 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801529A (en) * 1985-06-18 1989-01-31 Brandeis University Methods for isolating mutant microoganisms using microcapsules coated with indicator material
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US4811218A (en) 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
US5252494A (en) 1986-06-25 1993-10-12 Trustees Of Tufts College Fiber optic sensors, apparatus, and detection methods using controlled release polymers and reagent formulations held within a polymeric reaction matrix
US5254477A (en) 1986-06-25 1993-10-19 Trustees Of Tufts College Flourescence intramolecular energy transfer conjugate compositions and detection methods
US4822746A (en) 1986-06-25 1989-04-18 Trustees Of Tufts College Radiative and non-radiative energy transfer and absorbance modulated fluorescence detection methods and sensors
US5143853A (en) 1986-06-25 1992-09-01 Trustees Of Tufts College Absorbance modulated fluorescence detection methods and sensors
US4994372A (en) * 1987-01-14 1991-02-19 President And Fellows Of Harvard College DNA sequencing
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
JP2846018B2 (ja) 1988-01-21 1999-01-13 ジェネンテク，インコーポレイテッド 核酸配列の増幅および検出
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
SE8801070D0 (sv) * 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
AU3556789A (en) 1988-04-22 1989-11-24 Massachusetts Institute Of Technology Process for forming and using microdroplets
US5225332A (en) 1988-04-22 1993-07-06 Massachusetts Institute Of Technology Process for manipulation of non-aqueous surrounded microdroplets
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US6054270A (en) 1988-05-03 2000-04-25 Oxford Gene Technology Limited Analying polynucleotide sequences
GB8822228D0 (en) 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US4938876A (en) 1989-03-02 1990-07-03 Ohsol Ernest O Method for separating oil and water emulsions
US5629158A (en) * 1989-03-22 1997-05-13 Cemu Bitecknik Ab Solid phase diagnosis of medical conditions
JPH02299598A (ja) * 1989-04-14 1990-12-11 Ro Inst For Molecular Genetics & Geneteic Res 微視的サイズの別個の粒子と結合している核酸試料中のごく短い配列の全部または一部のオリゴヌクレチドプローブとのハイブリダイゼーションによる決定法
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5871928A (en) 1989-06-07 1999-02-16 Fodor; Stephen P. A. Methods for nucleic acid analysis
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
AU638762B2 (en) 1989-10-05 1993-07-08 Optein Inc Cell-free synthesis and isolation of novel genes and polypeptides
WO1991012342A1 (en) * 1990-02-16 1991-08-22 F. Hoffmann-La Roche Ag Improvements in the specificity and convenience of the polymerase chain reaction
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
JPH04223A (ja) * 1990-04-16 1992-01-06 Toshiba Corp 無線電話装置、その充電方法および無線電話充電システム
US5506100A (en) * 1990-10-11 1996-04-09 Indiana University Foundation Process and apparatus for fragmenting biomaterials
DK0552290T3 (da) * 1990-10-11 2000-05-29 Advanced Res & Tech Inst Fremgangsmåde og apparat til fragmentering af biologiske materialer
US5244636A (en) 1991-01-25 1993-09-14 Trustees Of Tufts College Imaging fiber optic array sensors, apparatus, and methods for concurrently detecting multiple analytes of interest in a fluid sample
US5320814A (en) 1991-01-25 1994-06-14 Trustees Of Tufts College Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample
US5250264A (en) 1991-01-25 1993-10-05 Trustees Of Tufts College Method of making imaging fiber optic sensors to concurrently detect multiple analytes of interest in a fluid sample
US5114984A (en) 1991-04-26 1992-05-19 Olin Corporation Process for producing an antimicrobially effective polyurethane
US5830663A (en) † 1991-08-10 1998-11-03 Medical Research Council In situ recombinant PCR within single cells
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
AU2674092A (en) * 1991-09-09 1993-04-05 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
EP0534858B2 (en) * 1991-09-24 2005-04-27 Keygene N.V. Selective restriction fragment amplification : a general method for DNA fingerprinting
US5270170A (en) 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
WO1993009668A1 (en) 1991-11-22 1993-05-27 Affymax Technology N.V. Combinatorial strategies for polymer synthesis
US5445971A (en) 1992-03-20 1995-08-29 Abbott Laboratories Magnetically assisted binding assays using magnetically labeled binding members
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
GB9210176D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
DE4223169C1 (de) 1992-07-10 1993-11-25 Ferring Arzneimittel Gmbh Verfahren zur Mikroverkapselung wasserlöslicher Wirkstoffe
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
US6114114A (en) 1992-07-17 2000-09-05 Incyte Pharmaceuticals, Inc. Comparative gene transcript analysis
RU2048522C1 (ru) 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
US5436143A (en) 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
IL104384A (en) 1993-01-13 1996-11-14 Yeda Res & Dev Method for screening catalytic non-enzyme polypeptides and proteins
US5298741A (en) 1993-01-13 1994-03-29 Trustees Of Tufts College Thin film fiber optic sensor array and apparatus for concurrent viewing and chemical sensing of a sample
IL108497A0 (en) 1993-02-01 1994-05-30 Seq Ltd Methods and apparatus for dna sequencing
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
EP0695368A4 (en) 1993-04-19 1996-05-08 Stuart A Kauffman RANDOM PROCESS FOR PRODUCING NEW CHEMICAL COMPOUNDS
NZ265818A (en) 1993-04-19 1997-09-22 Medisorb Technologies Internat Compositions of nucleic acids encapsulated with molecules that facilitate uptake and integration into living cells
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5482845A (en) 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
US5429807A (en) 1993-10-28 1995-07-04 Beckman Instruments, Inc. Method and apparatus for creating biopolymer arrays on a solid support surface
PT725682E (pt) * 1993-10-28 2002-09-30 Houston Advanced Res Ct Dispositivo poroso microfabricado de escoamento
AU8124694A (en) 1993-10-29 1995-05-22 Affymax Technologies N.V. In vitro peptide and antibody display libraries
GB9401833D0 (en) 1994-02-01 1994-03-30 Isis Innovation Method for discovering ligands
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
EP0804249A2 (en) 1994-03-15 1997-11-05 Brown University Research Foundation Polymeric gene delivery system
AU2187295A (en) * 1994-03-18 1995-10-09 General Hospital Corporation, The Cleaved amplified rflp detection methods
JP2556293B2 (ja) 1994-06-09 1996-11-20 日本電気株式会社 Ｍｏｓ ｏｔａ
US5512490A (en) 1994-08-11 1996-04-30 Trustees Of Tufts College Optical sensor, optical sensing apparatus, and methods for detecting an analyte of interest using spectral recognition patterns
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
WO1998053300A2 (en) 1997-05-23 1998-11-26 Lynx Therapeutics, Inc. System and apparaus for sequential processing of analytes
US6013445A (en) 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
FR2726286B1 (fr) 1994-10-28 1997-01-17 Genset Sa Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede
US5919673A (en) 1995-03-22 1999-07-06 The Scripps Research Institute One-pot enzymatic sulfation process using 3'-phosphoadenosine-5'-phosphosulfate and recycled phosphorylated adenosine intermediates
DE69621507T2 (de) * 1995-03-28 2003-01-09 Japan Science And Technology Corp., Kawaguchi Verfahren zur molekularen Indexierung von Genen unter Verwendung von Restriktionsenzymen
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
CA2219136A1 (en) 1995-04-24 1996-10-31 Chromaxome Corp. Methods for generating and screening novel metabolic pathways
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
DE19518505A1 (de) * 1995-05-19 1996-11-21 Max Planck Gesellschaft Verfahren zur Genexpressionsanalyse
US5690894A (en) 1995-05-23 1997-11-25 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
US5814444A (en) * 1995-06-07 1998-09-29 University Of Washington Methods for making and using single-chromosome amplfication libraries
US5910408A (en) 1995-06-07 1999-06-08 The General Hospital Corporation Catalytic DNA having ligase activity
US5989892A (en) 1995-06-14 1999-11-23 Tonen Corporation Microorganisms, demulsifiers and processes for breaking an emulsion
US5728529A (en) 1995-06-23 1998-03-17 Baylor College Of Medicine Alternative dye-labeled ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated DNA analysis
US6200737B1 (en) 1995-08-24 2001-03-13 Trustees Of Tufts College Photodeposition method for fabricating a three-dimensional, patterned polymer microstructure
US5843655A (en) 1995-09-18 1998-12-01 Affymetrix, Inc. Methods for testing oligonucleotide arrays
US5871697A (en) 1995-10-24 1999-02-16 Curagen Corporation Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
DE19646372C1 (de) 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Genotyp und Phänotyp koppelnde Verbindung
US5962228A (en) 1995-11-17 1999-10-05 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US5780231A (en) 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
ATE199572T1 (de) 1995-11-21 2001-03-15 Univ Yale Unimolekulare segmentamplifikation und bestimmung
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5633972A (en) 1995-11-29 1997-05-27 Trustees Of Tufts College Superresolution imaging fiber for subwavelength light energy generation and near-field optical microscopy
US5814524A (en) 1995-12-14 1998-09-29 Trustees Of Tufts College Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations
US5837196A (en) 1996-01-26 1998-11-17 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
US5851772A (en) 1996-01-29 1998-12-22 University Of Chicago Microchip method for the enrichment of specific DNA sequences
AU2264197A (en) * 1996-02-09 1997-08-28 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Restriction display (rd-pcr) of differentially expressed mrnas
AU2069597A (en) * 1996-03-04 1997-09-22 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
GB9608540D0 (en) 1996-04-25 1996-07-03 Medical Res Council Isolation of enzymes
US5712127A (en) 1996-04-29 1998-01-27 Genescape Inc. Subtractive amplification
US5770637A (en) * 1996-05-01 1998-06-23 Johnson & Johnson Vision Products, Inc. Anti-bacterial, UV absorbable, tinted, metal-chelating polymers
US6022688A (en) * 1996-05-13 2000-02-08 Sequenom, Inc. Method for dissociating biotin complexes
CA2256700A1 (en) * 1996-06-06 1997-12-11 Lynx Therapeutics, Inc. Sequencing by ligation of encoded adaptors
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
US6083693A (en) 1996-06-14 2000-07-04 Curagen Corporation Identification and comparison of protein-protein interactions that occur in populations
US5916524A (en) 1997-07-23 1999-06-29 Bio-Dot, Inc. Dispensing apparatus having improved dynamic range
GB9618050D0 (en) 1996-08-29 1996-10-09 Cancer Res Campaign Tech Global amplification of nucleic acids
US5846721A (en) 1996-09-19 1998-12-08 The Trustees Of Columbia University In The City Of New York Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
CA2236867A1 (en) 1996-09-27 1998-04-02 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
EP0937097B1 (en) 1996-11-06 2001-08-16 Sequenom, Inc. Compositions and methods for immobilizing nucleic acids to solid supports
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US6887665B2 (en) 1996-11-14 2005-05-03 Affymetrix, Inc. Methods of array synthesis
DE19648372A1 (de) 1996-11-22 1998-05-28 Serck Como Gmbh Verfahren und Vorrichtung zum Eindampfen von Abwässern
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US6310354B1 (en) * 1996-12-03 2001-10-30 Erkki Soini Method and a device for monitoring nucleic acid amplification reactions
US6060245A (en) * 1996-12-13 2000-05-09 Stratagene Methods and adaptors for generating specific nucleic acid populations
US20020172965A1 (en) * 1996-12-13 2002-11-21 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
KR100566859B1 (ko) 1997-01-21 2006-04-03 제너럴 하스피톨 코포레이션 Ｒｎａ-단백질 융합물을 이용한 단백질의 선별
CA2196496A1 (en) 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US6136543A (en) 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor
ATE273381T1 (de) 1997-02-12 2004-08-15 Eugene Y Chan Verfahren zur analyse von polymeren
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US5994068A (en) * 1997-03-11 1999-11-30 Wisconsin Alumni Research Foundation Nucleic acid indexing
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
AU6571598A (en) 1997-03-18 1998-10-12 Chromaxome Corporation Methods for screening compounds using encapsulated cells
US5891477A (en) * 1997-03-28 1999-04-06 Biohybrid Technologies, Inc. Non-steroidal anti-inflammatory agents inhibition of fibrotic response to an implanted device
EP0975802B1 (en) * 1997-04-01 2004-06-23 Manteia S.A. Method of nucleic acid sequencing
ATE364718T1 (de) * 1997-04-01 2007-07-15 Solexa Ltd Verfahren zur vervielfältigung von nukleinsäure
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6406845B1 (en) 1997-05-05 2002-06-18 Trustees Of Tuft College Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample
ES2163271T3 (es) * 1997-05-13 2002-01-16 Azign Bioscience As Procedimiento para clonar arnm y visualizar transcriptores diferencialmente expresados (dodets).
DK1019496T3 (da) * 1997-07-07 2005-01-10 Medical Res Council In vitro-sorteringsmetode
IL133411A (en) 1997-07-28 2003-09-17 Medical Biosystems Ltd Nucleic acid sequence analysis
JP3610231B2 (ja) * 1997-08-01 2005-01-12 キヤノン株式会社 反応場アレー、反応場アレーの製造方法、反応場アレーを用いた反応方法及び反応場アレーを用いた試料溶液中の物質の定量方法
EP0895083B1 (en) 1997-08-01 2009-09-23 Canon Kabushiki Kaisha Reaction site array, preparation of it, reaction process using it and quantitative determination method of substance in sample solution using it
EP0990049A1 (en) 1997-08-07 2000-04-05 Curagen Corporation Detection and confirmation of nucleic acid sequences by use of oligonucleotides comprising a subsequence hybridizing exactly to a known terminal sequence and a subsequence hybridizing to an unidentified sequence
US5961228A (en) * 1997-08-22 1999-10-05 Paxar Corporation Modular printer
US20010006630A1 (en) 1997-09-02 2001-07-05 Oron Yacoby-Zeevi Introducing a biological material into a patient
US6087099A (en) * 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6475722B1 (en) 1997-12-03 2002-11-05 Curagen Corporation Surface treatments for DNA processing devices
AU1623899A (en) 1997-12-04 1999-06-16 Packard Bioscience Company Methods of using probes for analyzing polynucleotide sequence
NL1007781C2 (nl) 1997-12-12 1999-06-15 Packard Instr Bv Microtiterplaat.
AU1926899A (en) 1997-12-19 1999-07-12 Affymetrix, Inc. Exploiting genomics in the search for new drugs
EP1600512B1 (en) 1997-12-22 2008-05-28 Hitachi Chemical Co., Ltd. mRNA purification on microplates
WO1999036576A1 (en) 1998-01-20 1999-07-22 Packard Bioscience Company Gel pad arrays and methods and systems for making them
KR100280219B1 (ko) * 1998-02-26 2001-04-02 이수빈 삼핵산 반복 서열을 이용한 신경정신 질환의 진단 방법 및 진단 시약
US5882874A (en) 1998-02-27 1999-03-16 The Trustees Of Columbia University In The City Of New York Reciprocal subtraction differential display
US6210910B1 (en) 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
US6378527B1 (en) 1998-04-08 2002-04-30 Chondros, Inc. Cell-culture and polymer constructs
WO1999053102A1 (en) 1998-04-16 1999-10-21 Packard Bioscience Company Analysis of polynucleotide sequence
AU3572799A (en) * 1998-04-24 1999-11-16 Genova Pharmaceuticals Corporation Function-based gene discovery
EP1428810A3 (en) 1998-05-15 2004-07-14 Oxford Gene Technology IP Limited Libraries of oligomers labelled with different tags
GB9811403D0 (en) 1998-05-27 1998-07-22 Isis Innovation Polynucleotide multimers and their use in hybridisation assays
GB9813216D0 (en) 1998-06-18 1998-08-19 Pyrosequencing Ab Reaction monitoring systems
CA2335951C (en) 1998-06-24 2013-07-30 Mark S. Chee Decoding of array sensors with microspheres
JP2981547B1 (ja) * 1998-07-02 1999-11-22 農林水産省食品総合研究所長 クロスフロー型マイクロチャネル装置及び同装置を用いたエマルションの生成または分離方法
WO2000004139A1 (en) 1998-07-17 2000-01-27 Mirus Corporation Micellar systems
DE69928265T3 (de) 1998-07-30 2013-11-28 Illumina Cambridge Ltd. Matrizen von biomolekülen und ihre verwendung in sequenzierung
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
GB9820185D0 (en) 1998-09-15 1998-11-11 Dynal As Method
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US6203989B1 (en) 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
CA2348696A1 (en) 1998-11-06 2000-05-18 Solexa Ltd. A method for reproducing molecular arrays
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
WO2000039343A1 (en) * 1998-12-31 2000-07-06 City Of Hope Method for detecting mutations in nucleic acids
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
GB9901475D0 (en) 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing
WO2000044491A2 (en) 1999-01-29 2000-08-03 Illumina, Inc. Centrifuging apparatus and method for separation of liquid phases and organic synthesis
US20020150909A1 (en) * 1999-02-09 2002-10-17 Stuelpnagel John R. Automated information processing in randomly ordered arrays
JP2002536665A (ja) 1999-02-09 2002-10-29 イルミナ インコーポレイテッド ランダムに並んだアレイにおける自動情報処理
WO2000048000A1 (en) 1999-02-09 2000-08-17 Illumina, Inc. Intrabead screening methods and compositions
AU779231B2 (en) * 1999-02-22 2005-01-13 Lynx Therapeutics, Inc. Polymorphic DNA fragments and uses thereof
US6255476B1 (en) 1999-02-22 2001-07-03 Pe Corporation (Ny) Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports
US6225061B1 (en) 1999-03-10 2001-05-01 Sequenom, Inc. Systems and methods for performing reactions in an unsealed environment
AU3174600A (en) * 1999-03-10 2000-09-28 Asm Scientific, Inc. A method for direct nucleic acid sequencing
JP2004512807A (ja) * 1999-03-18 2004-04-30 コンプリート　ゲノミックス　エイエス 可読情報を有するフラグメント鎖のクローニングおよび生成方法
GB9906477D0 (en) 1999-03-19 1999-05-12 Pyrosequencing Ab Liquid dispensing apparatus
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO2000058507A1 (en) 1999-03-30 2000-10-05 Solexa Ltd. Polynucleotide sequencing
GB9907813D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
GB9907812D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
US6284465B1 (en) * 1999-04-15 2001-09-04 Agilent Technologies, Inc. Apparatus, systems and method for locating nucleic acids bound to surfaces
ATE413467T1 (de) 1999-04-20 2008-11-15 Illumina Inc Detektion von nukleinsäurereaktionen auf kügelchen-arrays
US20030108867A1 (en) 1999-04-20 2003-06-12 Chee Mark S Nucleic acid sequencing using microsphere arrays
US6355431B1 (en) * 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
WO2000071992A1 (en) 1999-05-20 2000-11-30 Illumina, Inc. Method and apparatus for retaining and presenting at least one microsphere array to solutions and/or to optical imaging systems
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
EP2360270B1 (en) 1999-05-20 2016-11-09 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
US6300070B1 (en) * 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US6340589B1 (en) 1999-07-23 2002-01-22 Mj Research, Inc. Thin-well microplate and methods of making same
US6942968B1 (en) 1999-08-30 2005-09-13 Illumina, Inc. Array compositions for improved signal detection
GB9921155D0 (en) 1999-09-08 1999-11-10 Medical Res Council Selection system
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
GB9923324D0 (en) 1999-10-01 1999-12-08 Pyrosequencing Ab Separation apparatus and method
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
GB9929381D0 (en) 1999-12-10 2000-02-09 Pyrosequencing Ab A method of assessing the amount of nucleic acid in a sample
AU2438501A (en) 1999-12-21 2001-07-03 Government Of The United States Of America, As Represented By The Secretary Of The Navy, The Expression of proteins from amplified, immobilized nucleic acids
DE60041150D1 (de) 1999-12-23 2009-01-29 Illumina Inc Dekodierung von matrixsensoren mit mikrokugeln
JP4860869B2 (ja) * 1999-12-29 2012-01-25 オックスフォード ジーン テクノロジー アイピー リミティド 固相支持体上の複数のポリヌクレオチドを増幅し、検出する方法
WO2001053532A2 (en) 2000-01-20 2001-07-26 Rosetta Inpharmatics, Inc. Barcoded synthetic lethal screening to identify drug targets
CA2401962A1 (en) 2000-02-07 2001-08-09 Illumina, Inc. Nucleic acid detection methods using universal priming
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
EP1990428B1 (en) 2000-02-07 2010-12-22 Illumina, Inc. Nucleic acid detection methods using universal priming
WO2001059432A2 (en) 2000-02-10 2001-08-16 Illumina, Inc. Array of individual arrays as substrate for bead-based simultaneous processing of samples and manufacturing method therefor
EP1967595A3 (en) 2000-02-16 2008-12-03 Illumina, Inc. Parallel genotyping of multiple patient samples
US6714874B1 (en) * 2000-03-15 2004-03-30 Applera Corporation Method and system for the assembly of a whole genome using a shot-gun data set
JP3442338B2 (ja) 2000-03-17 2003-09-02 株式会社日立製作所 Ｄｎａ分析装置、ｄｎａ塩基配列決定装置、ｄｎａ塩基配列決定方法、および反応モジュール
CN1189159C (zh) * 2000-05-05 2005-02-16 欧莱雅 含水溶性美容活性组分水性核的微胶囊及含其的组合物
SE0001768D0 (sv) 2000-05-12 2000-05-12 Helen Andersson Mikrofluidisk flödescell för manipulering av partiklar
US6475736B1 (en) * 2000-05-23 2002-11-05 Variagenics, Inc. Methods for genetic analysis of DNA using biased amplification of polymorphic sites
US6808874B2 (en) 2000-06-13 2004-10-26 Cyclacel Ltd. Methods of monitoring enzyme activity
AU2001271662B2 (en) 2000-06-28 2006-07-27 Illumina, Inc. Composite arrays utilizing microspheres with a hybridization chamber
WO2002012897A2 (en) 2000-08-09 2002-02-14 Illumina, Inc. Automated information processing in randomly ordered arrays
WO2002016649A2 (en) 2000-08-25 2002-02-28 Illumina, Inc. Probes and decoder oligonucleotides
WO2002021128A2 (en) 2000-09-05 2002-03-14 Illumina, Inc. Cellular arrays comprising encoded cells
GB0021977D0 (en) 2000-09-07 2000-10-25 Pyrosequencing Ab Method of sequencing DNA
GB0022069D0 (en) * 2000-09-08 2000-10-25 Pyrosequencing Ab Method
GB0022458D0 (en) * 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
WO2002027029A2 (en) * 2000-09-27 2002-04-04 Lynx Therapeutics, Inc. Method for determining relative abundance of nucleic acid sequences
US6632610B2 (en) * 2000-10-12 2003-10-14 Gensat S.A. Methods of identification and isolation of polynucleotides containing nucleic acid differences
US20020172980A1 (en) * 2000-11-27 2002-11-21 Phan Brigitte Chau Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems
JP2002257070A (ja) 2001-02-28 2002-09-11 Toyota Industries Corp 真空ポンプにおける軸封構造
US6936264B2 (en) * 2001-03-05 2005-08-30 The Procter & Gamble Company Delivery of reactive agents via multiple emulsions for use in shelf stable products
US6996287B1 (en) 2001-04-20 2006-02-07 Adobe Systems, Inc. Method and apparatus for texture cloning
GB0114856D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selection by avidity capture
GB0114854D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
US20030096268A1 (en) * 2001-07-06 2003-05-22 Michael Weiner Method for isolation of independent, parallel chemical micro-reactions using a porous filter
US20030064400A1 (en) * 2001-08-24 2003-04-03 Li-Cor, Inc. Microfluidics system for single molecule DNA sequencing
US6956114B2 (en) 2001-10-30 2005-10-18 '454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6902921B2 (en) * 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
GB0127564D0 (en) * 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
EP2196544A1 (en) 2001-11-21 2010-06-16 Applied Biosystems, LLC Kit for ligation detection assays using codeable labels
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
JP4230364B2 (ja) * 2002-04-04 2009-02-25 バイオタージ アクチボラゲット 新しい方法
AU2003302770B2 (en) 2002-12-20 2007-07-12 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA
EP1583831A2 (en) * 2003-01-15 2005-10-12 Wyeth Novel high throughput method of generating and purifying labeled crna targets for gene expression analysis
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
EP1594950A4 (en) 2003-01-29 2007-07-25 454 Corp PRODUCT FOR THE PREPARATION OF SIMPLE STRANDED DNA BANKS
US8150627B2 (en) * 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
CA2531105C (en) * 2003-07-05 2015-03-17 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
WO2005073410A2 (en) 2004-01-28 2005-08-11 454 Corporation Nucleic acid amplification with continuous flow emulsion
US7785862B2 (en) 2005-04-07 2010-08-31 454 Life Sciences Corporation Thin film coated microwell arrays
US7682816B2 (en) 2005-04-07 2010-03-23 454 Life Sciences Corporation Thin film coated microwell arrays and methods of using same
CA2615323A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
EP2002367B1 (en) 2006-02-16 2017-03-29 454 Life Sciences Corporation System and method for correcting primer extension errors in nucleic acid sequence data
US8364417B2 (en) 2007-02-15 2013-01-29 454 Life Sciences Corporation System and method to correct out of phase errors in DNA sequencing data by use of a recursive algorithm
CN101506378A (zh) 2006-06-19 2009-08-12 约翰·霍普金斯大学 在油包水乳液中的微粒上的单分子pcr
WO2008011621A2 (en) 2006-07-21 2008-01-24 The Penn State Research Foundation Protein kinase c zeta inhibition to treat vascular permeability
US8617816B2 (en) 2007-03-16 2013-12-31 454 Life Sciences, A Roche Company System and method for detection of HIV drug resistant variants
EP3404009B1 (en) 2017-05-16 2019-12-25 Arkema France Method for manufacturing 1,4-bis(4-phenoxybenzoyl)benzene in supersaturation conditions

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9897986B2 (en) 2013-10-29 2018-02-20 Regal Beloit America, Inc. System and method for enabling a motor controller to communicate using multiple different communication protocols

Also Published As

Publication number Publication date
CA2513535C (en) 2012-06-12
WO2004070005B1 (en) 2005-01-20
JP2010142233A (ja) 2010-07-01
ATE546525T1 (de) 2012-03-15
AU2004209001B2 (en) 2007-10-11
US20050079510A1 (en) 2005-04-14
JP2007525151A (ja) 2007-09-06
ATE461291T1 (de) 2010-04-15
JP2010178744A (ja) 2010-08-19
ATE448302T1 (de) 2009-11-15
JP2006515522A (ja) 2006-06-01
EP1594950A2 (en) 2005-11-16
JP2013066475A (ja) 2013-04-18
US8012690B2 (en) 2011-09-06
EP2145955B1 (en) 2012-02-22
AU2004209426B2 (en) 2008-04-17
DE602004026033D1 (de) 2010-04-29
EP1594981A4 (en) 2008-03-05
WO2004070005A2 (en) 2004-08-19
DE602004024034D1 (de) 2009-12-24
EP1594981B1 (en) 2010-03-17
US20040185484A1 (en) 2004-09-23
EP2145955A2 (en) 2010-01-20
US20140162885A1 (en) 2014-06-12
EP2145955A3 (en) 2011-03-30
ES2338654T3 (es) 2010-05-11
AU2004209416B2 (en) 2007-08-02
CN101128601A (zh) 2008-02-20
US20160298175A1 (en) 2016-10-13
US8748102B2 (en) 2014-06-10
US20190203263A1 (en) 2019-07-04
US8765380B2 (en) 2014-07-01
US20110201526A1 (en) 2011-08-18
ES2338654T5 (es) 2017-12-11
JP4768598B2 (ja) 2011-09-07
CA2727850C (en) 2013-04-30
CA2513899A1 (en) 2005-01-13
EP1594980A4 (en) 2007-05-02
CA2513541A1 (en) 2004-08-19
ES2342665T3 (es) 2010-07-12
EP2159285A2 (en) 2010-03-03
WO2004070007A2 (en) 2004-08-19
CA2513535A1 (en) 2004-08-19
WO2004070007A3 (en) 2005-12-22
US20060134633A1 (en) 2006-06-22
US20110009275A1 (en) 2011-01-13
AU2004209416A1 (en) 2004-08-19
JP2007535892A (ja) 2007-12-13
US8158359B2 (en) 2012-04-17
CA2728746C (en) 2018-01-16
AU2004209426A1 (en) 2004-08-19
CA2728746A1 (en) 2005-01-13
JP2007523627A (ja) 2007-08-23
AU2004254552B2 (en) 2008-04-24
US20050130173A1 (en) 2005-06-16
US20080132693A1 (en) 2008-06-05
EP2159285B1 (en) 2012-09-26
CN102212614B (zh) 2013-12-25
ATE437945T1 (de) 2009-08-15
WO2004070005A3 (en) 2004-11-11
WO2005003375A2 (en) 2005-01-13
EP1997889A2 (en) 2008-12-03
EP2261372B1 (en) 2012-08-22
EP2261372A3 (en) 2011-04-06
CN101128601B (zh) 2011-06-08
JP5726155B2 (ja) 2015-05-27
WO2004069849A8 (en) 2007-04-19
DE602004022253D1 (de) 2009-09-10
JP2012231799A (ja) 2012-11-29
CA2513889A1 (en) 2004-08-19
AU2004209001A1 (en) 2004-08-19
EP1594980A2 (en) 2005-11-16
EP1997889A3 (en) 2009-09-23
US7323305B2 (en) 2008-01-29
JP2013090635A (ja) 2013-05-16
WO2005003375A3 (en) 2007-08-23
US20150099672A1 (en) 2015-04-09
US20130078638A1 (en) 2013-03-28
US10240192B2 (en) 2019-03-26
WO2004069849A3 (en) 2005-03-24
EP2159285A3 (en) 2011-06-29
US20120238475A1 (en) 2012-09-20
JP5495399B2 (ja) 2014-05-21
EP1594980B2 (en) 2017-08-16
EP1594981A2 (en) 2005-11-16
EP1590477A2 (en) 2005-11-02
WO2004069849A2 (en) 2004-08-19
US10982274B2 (en) 2021-04-20
US20090011959A1 (en) 2009-01-08
US8790876B2 (en) 2014-07-29
ES2380893T3 (es) 2012-05-21
CA2513899C (en) 2013-03-26
ES2330339T3 (es) 2009-12-09
EP1594980B1 (en) 2009-11-11
EP1590477A4 (en) 2008-04-09
JP4480715B2 (ja) 2010-06-16
ES2396245T3 (es) 2013-02-20
US20090048124A1 (en) 2009-02-19
EP1590477B1 (en) 2009-07-29
DE602004036672C5 (de) 2012-11-29
EP2261372A2 (en) 2010-12-15
US7842457B2 (en) 2010-11-30
AU2004254552A1 (en) 2005-01-13
JP5258822B2 (ja) 2013-08-07
US7244567B2 (en) 2007-07-17
CA2727850A1 (en) 2005-01-13
CN102212614A (zh) 2011-10-12
EP1594950A4 (en) 2007-07-25

Similar Documents

Publication Publication Date Title
JP4473878B2 (ja) 核酸を増幅および配列決定する方法
JP2007523627A6 (ja) 核酸を増幅および配列決定する方法
US7575865B2 (en) Methods of amplifying and sequencing nucleic acids
JP4727109B2 (ja) 核酸を配列決定する方法
JP4354184B2 (ja) 核酸の配列決定のための装置および方法
HK1118082A (en) Methods of amplifying and sequencing nucleic acids

Legal Events

Date Code Title Description
A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20090928

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20091228

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20100301

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20100305

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130312

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Ref document number: 4473878

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

S533 Written request for registration of change of name

Free format text: JAPANESE INTERMEDIATE CODE: R313533

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130312

Year of fee payment: 3

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term