ATE465274T1 - Verbesserte strategien zur sequenzierung komplexer genome unter verwendung von sequenziertechniken mit hohem durchsatz - Google Patents

Verbesserte strategien zur sequenzierung komplexer genome unter verwendung von sequenziertechniken mit hohem durchsatz

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Publication number
ATE465274T1
ATE465274T1 AT06757809T AT06757809T ATE465274T1 AT E465274 T1 ATE465274 T1 AT E465274T1 AT 06757809 T AT06757809 T AT 06757809T AT 06757809 T AT06757809 T AT 06757809T AT E465274 T1 ATE465274 T1 AT E465274T1
Authority
AT
Austria
Prior art keywords
sequence
fragments
primer
sequencing
genome
Prior art date
Application number
AT06757809T
Other languages
English (en)
Inventor
Eijk Michael Josephus Ther Van
Anker Preben Soerensen
Rene Cornelis Josephus Hogers
Original Assignee
Keygene Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Keygene Nv filed Critical Keygene Nv
Application granted granted Critical
Publication of ATE465274T1 publication Critical patent/ATE465274T1/de

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
AT06757809T 2005-06-23 2006-06-23 Verbesserte strategien zur sequenzierung komplexer genome unter verwendung von sequenziertechniken mit hohem durchsatz ATE465274T1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US69305305P 2005-06-23 2005-06-23
US71489705P 2005-09-08 2005-09-08
EP06075104 2006-01-16
US75903406P 2006-01-17 2006-01-17
PCT/NL2006/000312 WO2006137734A1 (en) 2005-06-23 2006-06-23 Improved strategies for sequencing complex genomes using high throughput sequencing technologies

Publications (1)

Publication Number Publication Date
ATE465274T1 true ATE465274T1 (de) 2010-05-15

Family

ID=36950154

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06757809T ATE465274T1 (de) 2005-06-23 2006-06-23 Verbesserte strategien zur sequenzierung komplexer genome unter verwendung von sequenziertechniken mit hohem durchsatz

Country Status (11)

Country Link
US (1) US20090142758A1 (de)
EP (1) EP1910563B1 (de)
JP (1) JP2008546405A (de)
CN (1) CN101278058A (de)
AT (1) ATE465274T1 (de)
AU (1) AU2006259990B2 (de)
CA (1) CA2613248A1 (de)
DE (1) DE602006013831D1 (de)
DK (1) DK1910562T3 (de)
ES (2) ES2344802T3 (de)
WO (1) WO2006137734A1 (de)

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DE602006018744D1 (de) 2005-06-23 2011-01-20 Keygene Nv Strategien mit hohem durchsatz zur identifizierung und zum nachweis von polymorphismen
CA2623539C (en) 2005-09-29 2015-12-15 Keygene N.V. High throughput screening of mutagenized populations
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
DK3404114T3 (da) 2005-12-22 2021-06-28 Keygene Nv Fremgangsmåde til detektering af AFLP-baseret polymorfisme med højt gennemløb
ES2394633T3 (es) * 2005-12-22 2013-02-04 Keygene N.V. Estrategias mejoradas para elaboración de perfiles de transcritos usando tecnologías de secuenciación de alto rendimiento
US7932029B1 (en) * 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
DK3239304T3 (da) 2006-04-04 2020-10-26 Keygene Nv Højgennemløbsdetektering af molekylære markører baseret på AFLP og sekventering med højt gennemløb
US8178300B2 (en) * 2006-07-12 2012-05-15 Keygene N.V. Method for the identification of the clonal source of a restriction fragment
WO2008093098A2 (en) * 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
AR066922A1 (es) 2007-06-08 2009-09-23 Monsanto Technology Llc Metodos de mejoramiento molecular del germoplasma de una planta por secuenciamiento dirigido
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
CN102272334B (zh) 2009-01-13 2014-08-20 关键基因股份有限公司 新基因组测序策略
EP2248914A1 (de) 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Die Verwendung von IIB-Restriktionsendonukleasen in Sequenzierungsanwendungen der zweiten Generation
CN101693918B (zh) * 2009-10-13 2012-01-04 东南大学 一种提高核酸内切酶v切割位置特异性的方法
WO2011071382A1 (en) * 2009-12-10 2011-06-16 Keygene N.V. Polymorfphic whole genome profiling
EP2333104A1 (de) 2009-12-11 2011-06-15 Lexogen GmbH RNA-Analyseverfahren
EP2354243A1 (de) 2010-02-03 2011-08-10 Lexogen GmbH Verfahren zur Komplexitätsminderung
JP5977234B2 (ja) * 2010-07-09 2016-08-24 セルゲンティス ビー.ブイ. 対象の3−dゲノム領域配列決定戦略
AU2012304328B2 (en) * 2011-09-09 2017-07-20 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
CA2848240C (en) 2011-09-16 2020-09-29 Lexogen Gmbh Nucleic acid transcription method
CN102373288B (zh) * 2011-11-30 2013-12-11 盛司潼 一种对目标区域进行测序的方法及试剂盒
CN103305503B (zh) * 2012-03-16 2016-05-11 江苏基谱生物科技发展有限公司 一种用于高通量测序的索引序列组
WO2014129894A1 (en) * 2013-02-19 2014-08-28 Cergentis B.V. Sequencing strategies for genomic regions of interest
CN104630202A (zh) * 2013-11-13 2015-05-20 北京大学 一种能够减小微量核酸物质整体扩增时产生偏倚的扩增方法
GB201409282D0 (en) * 2014-05-23 2014-07-09 Univ Sydney Tech Sequencing process
WO2016058121A1 (zh) * 2014-10-13 2016-04-21 深圳华大基因科技有限公司 一种核酸片段化方法和序列组合
CN107590362B (zh) * 2017-08-21 2019-12-06 武汉菲沙基因信息有限公司 一种基于长读序测序判断重叠组装正误的方法
CN110157781B (zh) * 2018-12-29 2020-08-21 广州凯普医药科技有限公司 一种重叠延伸PCR结合Sanger测序检测不连续多DNA位点的方法
WO2020157684A1 (en) * 2019-01-29 2020-08-06 Mgi Tech Co., Ltd. High coverage stlfr
CN114008213A (zh) * 2019-05-20 2022-02-01 阿瑞玛基因组学公司 用于增强基因组覆盖和保持空间邻近的邻接性的方法和组合物
CN111667883B (zh) * 2020-06-03 2021-01-22 四川大学 一种基于复合微单倍型焦磷酸测序图谱解析的法医学混合dna的分析方法

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EP0969102B1 (de) * 1991-09-24 2007-12-26 Keygene N.V. Primer, Sätze und Restriktions Fragmente und dessen Benutzung in selektive Restriktionsfragmentenamplifikation
CA2286864A1 (en) * 1997-01-10 1998-07-16 Pioneer Hi-Bred International, Inc. Hybridization-based genetic amplification and analysis
DE69801749T2 (de) * 1997-05-13 2002-07-04 Azign Bioscience As Kopenhagen Eine methode zum klonieren von mrna und darstellung von differentiell exprimierten transcripten (dodet)
CA2341157A1 (en) * 1998-08-31 2000-03-09 University Of Washington Stable isotope metabolic labeling for analysis of biopolymers
US6361947B1 (en) * 1998-10-27 2002-03-26 Affymetrix, Inc. Complexity management and analysis of genomic DNA
EP1141384A2 (de) * 1999-01-06 2001-10-10 Cornell Research Foundation, Inc. Verfahren zur beschleunigung der identifizierung von mononukleotid-polymorphismen und der ausrichtung von klonen bei genomischer sequenzierung
DE60038109T2 (de) * 1999-04-09 2008-11-27 Keygene N.V. Verfahren zur Analyse von AFLP Reaktionsmischungen unter Verwendung von Primer Verlängerungstechniken
US7217516B2 (en) * 2000-05-15 2007-05-15 Keygene N.V. Methods and kits comprising AFLP primers, and ramp primers with a part complementary to a compound microsatellite repeat and an anchor part complementary to nucleotides adjacent to the repeat
US20050064406A1 (en) * 2001-04-20 2005-03-24 Eugene Zabarovsky Methods for high throughput genome analysis using restriction site tagged microarrays
ES2342665T3 (es) * 2003-01-29 2010-07-12 454 Corporation Secuenciacion desde dos extremos.

Also Published As

Publication number Publication date
AU2006259990B2 (en) 2011-01-27
CN101278058A (zh) 2008-10-01
AU2006259990A1 (en) 2006-12-28
JP2008546405A (ja) 2008-12-25
ES2357549T3 (es) 2011-04-27
DE602006013831D1 (de) 2010-06-02
US20090142758A1 (en) 2009-06-04
EP1910563A1 (de) 2008-04-16
WO2006137734A1 (en) 2006-12-28
CA2613248A1 (en) 2006-12-28
ES2344802T3 (es) 2010-09-07
DK1910562T3 (da) 2011-03-21
EP1910563B1 (de) 2010-04-21

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