JP2016511243A - ポリヌクレオチドバーコード生成 - Google Patents

ポリヌクレオチドバーコード生成 Download PDF

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JP2016511243A
JP2016511243A JP2015557149A JP2015557149A JP2016511243A JP 2016511243 A JP2016511243 A JP 2016511243A JP 2015557149 A JP2015557149 A JP 2015557149A JP 2015557149 A JP2015557149 A JP 2015557149A JP 2016511243 A JP2016511243 A JP 2016511243A
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polynucleotide
library
sequence
partition
barcode
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JP2016511243A5 (enExample
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ベンジャミン・ハインドソン
ミアナ・ジャロスツ
ポール・ハーデンボル
マイケル・シュナール−レヴィン
ケヴィン・ネス
サージ・サクソノフ
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テンエックス・ジェノミクス・インコーポレイテッド
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Publication of JP2016511243A publication Critical patent/JP2016511243A/ja
Publication of JP2016511243A5 publication Critical patent/JP2016511243A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/5436—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2015557149A 2013-02-08 2014-02-07 ポリヌクレオチドバーコード生成 Pending JP2016511243A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2020206665A JP7686389B2 (ja) 2013-02-08 2020-12-14 ポリヌクレオチドバーコード生成

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201361762435P 2013-02-08 2013-02-08
US61/762,435 2013-02-08
US201361800223P 2013-03-15 2013-03-15
US61/800,223 2013-03-15
US201361840403P 2013-06-27 2013-06-27
US61/840,403 2013-06-27
US201361844804P 2013-07-10 2013-07-10
US61/844,804 2013-07-10
PCT/US2014/015427 WO2014124338A1 (en) 2013-02-08 2014-02-07 Polynucleotide barcode generation

Related Child Applications (2)

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JP2019074785A Division JP2019107035A (ja) 2013-02-08 2019-04-10 ポリヌクレオチドバーコード生成
JP2020206665A Division JP7686389B2 (ja) 2013-02-08 2020-12-14 ポリヌクレオチドバーコード生成

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JP2016511243A true JP2016511243A (ja) 2016-04-14
JP2016511243A5 JP2016511243A5 (enExample) 2017-04-06

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JP2015557149A Pending JP2016511243A (ja) 2013-02-08 2014-02-07 ポリヌクレオチドバーコード生成
JP2019074785A Pending JP2019107035A (ja) 2013-02-08 2019-04-10 ポリヌクレオチドバーコード生成
JP2020206665A Active JP7686389B2 (ja) 2013-02-08 2020-12-14 ポリヌクレオチドバーコード生成
JP2023034686A Pending JP2023063359A (ja) 2013-02-08 2023-03-07 ポリヌクレオチドバーコード生成

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JP2019074785A Pending JP2019107035A (ja) 2013-02-08 2019-04-10 ポリヌクレオチドバーコード生成
JP2020206665A Active JP7686389B2 (ja) 2013-02-08 2020-12-14 ポリヌクレオチドバーコード生成
JP2023034686A Pending JP2023063359A (ja) 2013-02-08 2023-03-07 ポリヌクレオチドバーコード生成

Country Status (10)

Country Link
US (8) US9388465B2 (enExample)
EP (4) EP3862435A1 (enExample)
JP (4) JP2016511243A (enExample)
KR (3) KR20230003659A (enExample)
CN (2) CN105102697A (enExample)
AU (1) AU2014214682B2 (enExample)
BR (1) BR112015019159A2 (enExample)
CA (2) CA2900543C (enExample)
DK (1) DK2954065T3 (enExample)
WO (2) WO2014124338A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019103415A (ja) * 2017-12-11 2019-06-27 株式会社日立製作所 単一細胞の網羅的３’末端遺伝子発現解析法
JP2025029115A (ja) * 2017-08-01 2025-03-05 イルミナ インコーポレイテッド ヌクレオチド配列決定のためのヒドロゲルビーズ

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