DE60040794D1 - Verfahren zur Immobilisierung von Oligonukleotiden auf festem Trägermaterial - Google Patents
Verfahren zur Immobilisierung von Oligonukleotiden auf festem TrägermaterialInfo
- Publication number
- DE60040794D1 DE60040794D1 DE60040794T DE60040794T DE60040794D1 DE 60040794 D1 DE60040794 D1 DE 60040794D1 DE 60040794 T DE60040794 T DE 60040794T DE 60040794 T DE60040794 T DE 60040794T DE 60040794 D1 DE60040794 D1 DE 60040794D1
- Authority
- DE
- Germany
- Prior art keywords
- solid support
- nucleic acid
- immobilization
- oligonucleotides
- support material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Hematology (AREA)
- General Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US15126799P | 1999-08-27 | 1999-08-27 | |
US17784400P | 2000-01-25 | 2000-01-25 | |
PCT/US2000/023627 WO2001016372A1 (en) | 1999-08-27 | 2000-08-28 | Methods of immobilizing ligands on solid supports and apparatus and methods of use therefor |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60040794D1 true DE60040794D1 (de) | 2008-12-24 |
Family
ID=26848471
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60040794T Expired - Lifetime DE60040794D1 (de) | 1999-08-27 | 2000-08-28 | Verfahren zur Immobilisierung von Oligonukleotiden auf festem Trägermaterial |
Country Status (7)
Country | Link |
---|---|
US (3) | US6492118B1 (de) |
EP (1) | EP1208238B1 (de) |
JP (1) | JP2003508763A (de) |
AT (1) | ATE414171T1 (de) |
CA (1) | CA2382121A1 (de) |
DE (1) | DE60040794D1 (de) |
WO (1) | WO2001016372A1 (de) |
Families Citing this family (84)
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US6673908B1 (en) * | 1999-02-22 | 2004-01-06 | Nuvelo, Inc. | Tumor necrosis factor receptor 2 |
US20020012971A1 (en) * | 2000-03-20 | 2002-01-31 | Mehta Tammy Burd | PCR compatible nucleic acid sieving medium |
WO2002027026A2 (en) * | 2000-09-28 | 2002-04-04 | Matrix Technologies Corporation | Improved support for solid phase hybridization assays |
CN100357450C (zh) * | 2000-10-23 | 2007-12-26 | 因哲尼控股公司 | 检测样品中核酸片段突变的方法和装置 |
EP1245272A1 (de) * | 2001-03-30 | 2002-10-02 | Ucb S.A. | Herstellung und Verwendung von Substraten |
US7297311B2 (en) * | 2001-06-29 | 2007-11-20 | Sysmex Corporation | Automatic smear preparing apparatus and automatic sample analysis system having the same |
EP1417337B1 (de) * | 2001-07-11 | 2009-03-04 | Baylor College of Medicine | Verfahren und vorrichtungen auf grundlage einer neuen form eines nukleinsäureduplex auf einer oberfläche |
US10272409B2 (en) | 2001-07-11 | 2019-04-30 | Michael E. Hogan | Methods and devices based upon a novel form of nucleic acid duplex on a surface |
ATE323885T1 (de) * | 2001-07-16 | 2006-05-15 | Dr Chip Biotechnology Inc | Immobilisierung von oligonukleotiden durch kovalente bindung |
US6995248B2 (en) | 2001-07-16 | 2006-02-07 | Dr. Chip Biotechnology, Inc. | Immobilization of nucleic acids |
RU2206575C2 (ru) * | 2001-07-25 | 2003-06-20 | Институт молекулярной биологии им. В.А. Энгельгардта РАН | Композиция для иммобилизации биологических макромолекул в гидрогелях, способ приготовления композиции, биочип, способ проведения пцр на биочипе |
WO2003078603A2 (en) * | 2002-03-15 | 2003-09-25 | Board Of Regents, The University Of Texas System | Strong gene sets for glioma classification |
US7371852B2 (en) * | 2003-01-22 | 2008-05-13 | Serenex, Inc. | Alkyl-linked nucleotide compositions |
SE0300624D0 (sv) * | 2003-03-05 | 2003-03-05 | Amersham Biosciences Ab | A method of preparing affinity ligands |
US20050112786A1 (en) * | 2003-11-25 | 2005-05-26 | Qing Wang | Method of immobilizing a substance of interest to a solid phase |
TW200521433A (en) * | 2003-12-22 | 2005-07-01 | We Gene Technologies Inc | Cell detection chip and fabricating method thereof and method of detecting cell |
US7693825B2 (en) * | 2004-03-31 | 2010-04-06 | Google Inc. | Systems and methods for ranking implicit search results |
GB0410448D0 (en) * | 2004-05-11 | 2004-06-16 | Hammersmith Imanet Ltd | Purification methods |
US7579224B2 (en) * | 2005-01-21 | 2009-08-25 | Semiconductor Energy Laboratory Co., Ltd. | Method for manufacturing a thin film semiconductor device |
US20070238163A1 (en) * | 2006-04-05 | 2007-10-11 | Golova Julia B | Covalent attachment of biomolecules to solid supports by a polymerization method |
US8241697B2 (en) | 2007-12-20 | 2012-08-14 | Abbott Point Of Care Inc. | Formation of immobilized biological layers for sensing |
US8268604B2 (en) * | 2007-12-20 | 2012-09-18 | Abbott Point Of Care Inc. | Compositions for forming immobilized biological layers for sensing |
JP2009156767A (ja) * | 2007-12-27 | 2009-07-16 | National Institute Of Advanced Industrial & Technology | タンパク質アレイ基板 |
RU2451010C1 (ru) * | 2011-01-11 | 2012-05-20 | Закрытое Акционерное Общество "Ива Фарм" | Палладиево-медные катализаторы гомогенного селективного окисления тиольных групп, комбинация и композиция на их основе и способ терапевтического воздействия |
US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US20150005199A1 (en) * | 2012-08-14 | 2015-01-01 | 10X Technologies, Inc. | Compositions and methods for sample processing |
US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
CA2881685C (en) | 2012-08-14 | 2023-12-05 | 10X Genomics, Inc. | Microcapsule compositions and methods |
US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US20140378349A1 (en) * | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US9567631B2 (en) | 2012-12-14 | 2017-02-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
CA2900543C (en) | 2013-02-08 | 2023-01-31 | 10X Genomics, Inc. | Partitioning and processing of analytes and other species |
WO2014138028A1 (en) * | 2013-03-04 | 2014-09-12 | The Scripps Research Institute | Liquid array platform for multiplexed analysis of molecule-protein interactions |
ES2537097B1 (es) * | 2013-10-30 | 2016-04-05 | Universitat Politècnica De València | Metodo de activación química superficial de un soporte sólido en base silicio mediante anclaje covalente directo de al menos una biomolécula de ácido nucleico. |
US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
CN106413896B (zh) | 2014-04-10 | 2019-07-05 | 10X基因组学有限公司 | 用于封装和分割试剂的流体装置、系统和方法及其应用 |
JP6838969B2 (ja) | 2014-06-26 | 2021-03-03 | 10エックス ジェノミクス, インコーポレイテッド | 個々の細胞または細胞集団由来の核酸の分析方法 |
US20160122817A1 (en) | 2014-10-29 | 2016-05-05 | 10X Genomics, Inc. | Methods and compositions for targeted nucleic acid sequencing |
US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
CN112126675B (zh) | 2015-01-12 | 2022-09-09 | 10X基因组学有限公司 | 用于制备核酸测序文库的方法和系统以及用其制备的文库 |
KR20170119710A (ko) | 2015-02-24 | 2017-10-27 | 10엑스 제노믹스, 인크. | 표적화된 핵산 서열 커버리지 방법 |
EP4286516A3 (de) | 2015-02-24 | 2024-03-06 | 10X Genomics, Inc. | Partitionsverarbeitungsverfahren und -systeme |
US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
SG11201804086VA (en) | 2015-12-04 | 2018-06-28 | 10X Genomics Inc | Methods and compositions for nucleic acid analysis |
JP6735348B2 (ja) | 2016-02-11 | 2020-08-05 | 10エックス ジェノミクス, インコーポレイテッド | 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体 |
WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
EP3545314A4 (de) * | 2016-11-28 | 2020-10-07 | Dana-Farber Cancer Institute, Inc. | Reagenzien und verfahren zur analyse von proteinen und metaboliten, die durch kovalente sonden angegriffen werden |
US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
WO2018140966A1 (en) | 2017-01-30 | 2018-08-02 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
US10844372B2 (en) | 2017-05-26 | 2020-11-24 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
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US10837047B2 (en) | 2017-10-04 | 2020-11-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
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Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5927900A (ja) | 1982-08-09 | 1984-02-14 | Wakunaga Seiyaku Kk | 固定化オリゴヌクレオチド |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
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-
2000
- 2000-08-28 US US09/649,637 patent/US6492118B1/en not_active Expired - Lifetime
- 2000-08-28 AT AT00957879T patent/ATE414171T1/de not_active IP Right Cessation
- 2000-08-28 DE DE60040794T patent/DE60040794D1/de not_active Expired - Lifetime
- 2000-08-28 WO PCT/US2000/023627 patent/WO2001016372A1/en active IP Right Grant
- 2000-08-28 EP EP00957879A patent/EP1208238B1/de not_active Expired - Lifetime
- 2000-08-28 JP JP2001520917A patent/JP2003508763A/ja active Pending
- 2000-08-28 CA CA002382121A patent/CA2382121A1/en not_active Abandoned
-
2002
- 2002-08-01 US US10/210,400 patent/US20030143570A1/en not_active Abandoned
- 2002-08-01 US US10/210,307 patent/US20030143569A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
JP2003508763A (ja) | 2003-03-04 |
US20030143569A1 (en) | 2003-07-31 |
WO2001016372A9 (en) | 2002-09-12 |
US20030143570A1 (en) | 2003-07-31 |
EP1208238B1 (de) | 2008-11-12 |
CA2382121A1 (en) | 2001-03-08 |
ATE414171T1 (de) | 2008-11-15 |
US6492118B1 (en) | 2002-12-10 |
WO2001016372A1 (en) | 2001-03-08 |
EP1208238A1 (de) | 2002-05-29 |
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