ATE351902T1 - Oberflächentransfektion und expressionsverfahren - Google Patents

Oberflächentransfektion und expressionsverfahren

Info

Publication number
ATE351902T1
ATE351902T1 AT01991560T AT01991560T ATE351902T1 AT E351902 T1 ATE351902 T1 AT E351902T1 AT 01991560 T AT01991560 T AT 01991560T AT 01991560 T AT01991560 T AT 01991560T AT E351902 T1 ATE351902 T1 AT E351902T1
Authority
AT
Austria
Prior art keywords
nucleic acids
cells
present
expression process
expression
Prior art date
Application number
AT01991560T
Other languages
English (en)
Inventor
Michael D Uhler
Original Assignee
Univ Michigan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/960,454 external-priority patent/US6897067B2/en
Application filed by Univ Michigan filed Critical Univ Michigan
Application granted granted Critical
Publication of ATE351902T1 publication Critical patent/ATE351902T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells
AT01991560T 2000-11-03 2001-11-02 Oberflächentransfektion und expressionsverfahren ATE351902T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US24589200P 2000-11-03 2000-11-03
US30555201P 2001-07-13 2001-07-13
US09/960,454 US6897067B2 (en) 2000-11-03 2001-09-21 Surface transfection and expression procedure
US10/002,802 US7056741B2 (en) 2000-11-03 2001-11-02 Surface transfection and expression procedure

Publications (1)

Publication Number Publication Date
ATE351902T1 true ATE351902T1 (de) 2007-02-15

Family

ID=46204296

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01991560T ATE351902T1 (de) 2000-11-03 2001-11-02 Oberflächentransfektion und expressionsverfahren

Country Status (9)

Country Link
US (1) US7056741B2 (de)
EP (1) EP1330516B1 (de)
JP (1) JP2005505231A (de)
KR (1) KR20030093185A (de)
AT (1) ATE351902T1 (de)
AU (1) AU2002231281B2 (de)
CA (1) CA2427916C (de)
DE (1) DE60126115T2 (de)
WO (1) WO2002042447A2 (de)

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US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6890556B1 (en) 2001-02-14 2005-05-10 Northwestern University Controlled surface-associated delivery of genes and oligonucleotides
US7029697B2 (en) 2001-02-14 2006-04-18 Northwestern University Controlled surface-associated delivery of genes and oligonucleotides
EP1497434A4 (de) * 2002-04-15 2005-08-24 Merck & Co Inc Matrixanalyse der genexpression in zellen (magec)
US7045281B2 (en) 2002-09-20 2006-05-16 Cell & Molecular Technologies, Inc. Method for using division arrested cells in screening assays
US20040138154A1 (en) 2003-01-13 2004-07-15 Lei Yu Solid surface for biomolecule delivery and high-throughput assay
EP2287341B1 (de) 2003-12-01 2013-02-13 Life Technologies Corporation Rekombinationsstellen enthaltende Nukleinsäuremoleküle und Verfahren zur Verwendung davon
CN101124316A (zh) * 2004-12-17 2008-02-13 日东电工株式会社 用于转染真核细胞的固定的可降解阳离子聚合物
KR100782578B1 (ko) * 2006-03-03 2007-12-06 연세대학교 산학협력단 세포 주기조절을 통한 세포 형질전환 효과 증진 방법
US20080193931A1 (en) * 2007-02-08 2008-08-14 Weiwei Li Method of rapidly quantifying global DNA methylation
US20110045058A1 (en) * 2007-11-20 2011-02-24 Beth Israel Deaconess Medical Center Transfection reagent
US9677078B2 (en) 2012-10-08 2017-06-13 Lipocalyx Gmbh Carboxylated polyamine derivatives as transfection reagents
US10760040B1 (en) 2014-07-03 2020-09-01 NanoCav, LLC Mechanical transfection devices and methods
US10081816B1 (en) 2014-07-03 2018-09-25 Nant Holdings Ip, Llc Mechanical transfection devices and methods
EP3199239A1 (de) * 2016-01-27 2017-08-02 Baden-Württemberg Stiftung gGmbH Verfahren und vorrichtung zur räumlich gesteuerten parallelen transfektion - schwimmende bohrlöcher
US10294454B2 (en) 2016-08-24 2019-05-21 General Electric Company Methods and kits for cell activation
EP3580556B1 (de) * 2017-02-13 2021-04-07 The Regents of The University of California Vorrichtung und verfahren zur intrazellulären freisetzung einer biomolekularen ladung durch schallwellenexposition

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US5352605A (en) * 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5583024A (en) * 1985-12-02 1996-12-10 The Regents Of The University Of California Recombinant expression of Coleoptera luciferase
AT401526B (de) * 1993-02-10 1996-09-25 Scheirer Winfried Reagenzlösung zur stabilisierung der lumineszenz bei der luciferasemessung
AU687961B2 (en) 1993-11-19 1998-03-05 Biotechnology Research And Development Corporation Chimeric regulatory regions and gene cassettes for expression of genes in plants
NZ279995A (en) * 1994-01-21 1998-04-27 Powderject Vaccines Inc Gas driven gene delivery instrument with a conical exit nozzle and a cartridge with an arcuate, linear passage
ES2316148T3 (es) * 1994-03-23 2009-04-01 Ohio University Acidos nucleicos compactados y su suministro a celulas.
US5837533A (en) * 1994-09-28 1998-11-17 American Home Products Corporation Complexes comprising a nucleic acid bound to a cationic polyamine having an endosome disruption agent
US5811274A (en) * 1994-12-09 1998-09-22 The Regents Of The University Of Michigan Methods, compositions and apparatus for cell transfection
EP0874910A4 (de) * 1995-06-07 1999-04-21 Life Technologies Inc Durch peptide erhöhte kationische lipid transfektionen
WO1997018317A1 (fr) * 1995-11-13 1997-05-22 Commissariat A L'energie Atomique Complexe proteique dodecaedrique adenoviral, composition le contenant et ses applications
AU2112697A (en) * 1996-02-09 1997-08-28 Pi-Wan Cheng Receptor ligand-facilitated delivery of biologically active molecules
CA2258154A1 (en) * 1996-06-24 1997-12-31 Prizm Pharmaceuticals, Inc. Heparinized medical devices containing heparin-binding growth factor conjugates
US5998136A (en) * 1996-08-19 1999-12-07 Arcaris, Inc. Selection systems and methods for identifying genes and gene products involved in cell proliferation
US5976796A (en) * 1996-10-04 1999-11-02 Loma Linda University Construction and expression of renilla luciferase and green fluorescent protein fusion genes
CN1181422A (zh) * 1996-10-31 1998-05-13 上海市肿瘤研究所 与生长因子受体结合的多肽所构建的基因转移载体
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
JP2002504812A (ja) 1997-05-21 2002-02-12 クローンテック ラボラトリーズ，インコーポレイテッド 核酸アレイ
US6074859A (en) * 1997-07-08 2000-06-13 Kikkoman Corporation Mutant-type bioluminescent protein, and process for producing the mutant-type bioluminescent protein
EP1068356B8 (de) 1998-04-03 2007-01-03 Adnexus Therapeutics, Inc. Adressierbare protein arrays
WO1999055886A1 (en) 1998-04-24 1999-11-04 Genova Pharmaceuticals Corporation Function-based gene discovery
US5965352A (en) * 1998-05-08 1999-10-12 Rosetta Inpharmatics, Inc. Methods for identifying pathways of drug action
US6534262B1 (en) 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
GB9815819D0 (en) 1998-07-22 1998-09-16 Secr Defence Transferring materials into cells and a microneedle array
US6544790B1 (en) * 1999-09-17 2003-04-08 Whitehead Institute For Biomedical Research Reverse transfection method

Also Published As

Publication number Publication date
EP1330516A2 (de) 2003-07-30
DE60126115D1 (de) 2007-03-08
CA2427916C (en) 2012-01-17
US7056741B2 (en) 2006-06-06
DE60126115T2 (de) 2007-08-23
EP1330516B1 (de) 2007-01-17
JP2005505231A (ja) 2005-02-24
CA2427916A1 (en) 2002-05-30
AU2002231281B2 (en) 2008-02-07
WO2002042447A3 (en) 2003-03-20
US20020197720A1 (en) 2002-12-26
WO2002042447A2 (en) 2002-05-30
KR20030093185A (ko) 2003-12-06

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