ATE191237T1 - Verfahren zur immobilisierung von nukleinsäuresonden an polystyroloberflächen - Google Patents

Verfahren zur immobilisierung von nukleinsäuresonden an polystyroloberflächen

Info

Publication number
ATE191237T1
ATE191237T1 AT93902855T AT93902855T ATE191237T1 AT E191237 T1 ATE191237 T1 AT E191237T1 AT 93902855 T AT93902855 T AT 93902855T AT 93902855 T AT93902855 T AT 93902855T AT E191237 T1 ATE191237 T1 AT E191237T1
Authority
AT
Austria
Prior art keywords
nucleic acid
polystyrene surfaces
acid probe
base
immobilizing nucleic
Prior art date
Application number
AT93902855T
Other languages
English (en)
Inventor
Patrick Sheridan
Chu-An Chang
Joyce Running
Original Assignee
Chiron Diagnostics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Diagnostics Corp filed Critical Chiron Diagnostics Corp
Application granted granted Critical
Publication of ATE191237T1 publication Critical patent/ATE191237T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT93902855T 1991-12-23 1992-12-22 Verfahren zur immobilisierung von nukleinsäuresonden an polystyroloberflächen ATE191237T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/813,338 US5747244A (en) 1991-12-23 1991-12-23 Nucleic acid probes immobilized on polystyrene surfaces
PCT/US1992/011343 WO1993013224A1 (en) 1991-12-23 1992-12-22 Process for immobilizing nucleic acid probes on polystyrene surfaces

Publications (1)

Publication Number Publication Date
ATE191237T1 true ATE191237T1 (de) 2000-04-15

Family

ID=25212094

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93902855T ATE191237T1 (de) 1991-12-23 1992-12-22 Verfahren zur immobilisierung von nukleinsäuresonden an polystyroloberflächen

Country Status (8)

Country Link
US (2) US5747244A (de)
EP (1) EP0620864B1 (de)
JP (2) JP3515781B2 (de)
AT (1) ATE191237T1 (de)
AU (1) AU3427693A (de)
CA (1) CA2125145A1 (de)
DE (1) DE69230864T2 (de)
WO (1) WO1993013224A1 (de)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100361933B1 (ko) 1993-09-08 2003-02-14 라 졸라 파마슈티칼 컴파니 화학적으로정의된비중합성결합가플랫폼분자및그것의콘주게이트
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6821727B1 (en) 1993-11-15 2004-11-23 Applera Corporation Hybridization assay using self-quenching fluorescence probe
US5853974A (en) 1995-06-07 1998-12-29 Chiron Corporation Enhancement of alkaline phosphatase with SDS in chemiluminescent substrates
US7825237B2 (en) 1996-05-03 2010-11-02 Applied Biosystems, Llc Oligonucleotides and analogs labeled with energy transfer dyes
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US7388092B2 (en) 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US5863727A (en) * 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5837466A (en) * 1996-12-16 1998-11-17 Vysis, Inc. Devices and methods for detecting nucleic acid analytes in samples
US5958696A (en) * 1997-07-17 1999-09-28 Boehringer Ingelheim Pharmaceuticals, Inc. Quantitative solid phase helicase assay
CA2313641A1 (en) * 1997-12-12 1999-06-24 Digene Corporation Universal collection medium
CA2321100A1 (en) * 1998-02-19 1999-08-26 Edvotek Articles of manufacture and methods for staining biomolecules
US6458953B1 (en) * 1998-12-09 2002-10-01 La Jolla Pharmaceutical Company Valency platform molecules comprising carbamate linkages
US6623919B1 (en) 1999-02-03 2003-09-23 Ortho-Clinical Diagnostics, Inc Oligonucleotide primers for efficient multiplex detection of hepatitis C virus (HCV) and human immunodeficiency virus (HIV) and methods of use thereof
US6638714B1 (en) * 1999-02-03 2003-10-28 Ortho-Clinical Diagnostics, Inc. Oligonucleotide primers for efficient detection of hepatitis C virus (HCV) and methods of use thereof
CN1762990A (zh) * 1999-06-08 2006-04-26 拉卓拉药物公司 包含氨基氧基的化合价平台分子
US6358684B1 (en) 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
US6218124B1 (en) 1999-08-27 2001-04-17 Pe Corporation Method for detecting oligonucleotides using UV light source
AU7078200A (en) 1999-08-27 2001-03-26 United States Of America, Represented By The Secretary, Department Of Health And Human Services, The Polypeptides, comprising il-6 ligand-binding receptor domains and related nucleic acids, antibodies, compositions, and methods of use
US6503736B1 (en) * 1999-11-12 2003-01-07 BIOMéRIEUX, INC. Antibodies to crosslinkers and methods for using the same
US7109024B2 (en) * 1999-11-15 2006-09-19 Dr. Chip Biotechnology Inc. Biomolecule-bound substrates
US20040146918A1 (en) * 2000-02-18 2004-07-29 Weiner Michael L. Hybrid nucleic acid assembly
US20030211496A1 (en) * 2000-03-29 2003-11-13 Getts Robert C Quality control reagents for nucleic acid microarrays
WO2001093914A2 (en) * 2000-06-08 2001-12-13 La Jolla Pharmaceutical Company Multivalent platform molecules comprising high molecular weight polyethylene oxide
US7601497B2 (en) 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
BR0106969A (pt) * 2000-07-05 2004-07-13 Cuno Inc Método de fabricação de um substrato multicelular e substrato multicelular
US20030219816A1 (en) * 2001-07-02 2003-11-27 Keith Solomon Composite microarray slides
US6890483B2 (en) * 2000-07-05 2005-05-10 Cuno Incorporated Non-luminescent substrate
GB0016836D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (1)
EP1360323A4 (de) * 2000-09-20 2007-07-18 Datascope Investment Corp Nukleinsäurenachweis durch dendrimermarkierung
JP2002125688A (ja) * 2000-10-30 2002-05-08 Tosoh Corp Ｃ型肝炎ウイルスの高感度検出のためのオリゴヌクレオチドおよび検出法
US8964604B2 (en) * 2000-12-26 2015-02-24 Polycom, Inc. Conference endpoint instructing conference bridge to dial phone number
US6927029B2 (en) 2001-12-03 2005-08-09 Agilent Technologies, Inc. Surface with tethered polymeric species for binding biomolecules
CA2480673A1 (en) * 2002-03-29 2003-10-09 Genentech, Inc. Methods and compositions for detection and quantitation of nucleic acid analytes
EP1387169B1 (de) * 2002-08-02 2006-05-24 Sony Deutschland GmbH Verfahren zur Bindung hydophiler Substanzen an hydrophile Makromoleküle und Immobilisierung derselben auf hydrophoben Oberflächen
WO2004015075A2 (en) * 2002-08-08 2004-02-19 Dharmacon, Inc. Short interfering rnas having a hairpin structure containing a non-nucleotide loop
US20040043478A1 (en) * 2002-08-29 2004-03-04 Li-Wei Hsu Plastic slides for the fabrication of biochips
EP1576351A4 (de) * 2002-11-26 2010-06-23 Univ Utah Res Found Mikroporöse materialien; verfahren undartikel zur lokalisierung und quantifizierung von analyten
US7597936B2 (en) * 2002-11-26 2009-10-06 University Of Utah Research Foundation Method of producing a pigmented composite microporous material
US7833714B1 (en) * 2003-04-11 2010-11-16 Affymetrix, Inc. Combinatorial affinity selection
US7943388B2 (en) * 2003-11-14 2011-05-17 3M Innovative Properties Company Acoustic sensors and methods
US7169933B2 (en) * 2003-11-14 2007-01-30 3M Innovative Properties Company N-sulfonylaminocarbonyl containing compounds
US7361767B2 (en) * 2003-11-14 2008-04-22 3M Innovative Properties Company N-sulfonyldicarboximide containing tethering compounds
WO2005049590A2 (en) * 2003-11-14 2005-06-02 3M Innovative Properties Company N-sulfonylaminocarbonyl containing compounds
US7423155B2 (en) * 2003-11-14 2008-09-09 3M Innovative Properties Company N-sulfonyldicarboximide containing tethering compounds
US7482122B1 (en) * 2004-10-19 2009-01-27 Hai Xing Chen Method of signal enhancement for measurement of nucleic acid sequences
US20060147940A1 (en) * 2004-12-30 2006-07-06 Affymetrix, Inc. Combinatorial affinity selection
US8632970B2 (en) 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
EP1880206B1 (de) * 2005-05-09 2012-11-14 Affymetrix, Inc. Multiplex-einfang von nukleinsäuren
US7803541B2 (en) * 2005-05-12 2010-09-28 Panomics, Inc. Multiplex branched-chain DNA assays
DK2500439T4 (da) 2005-06-20 2017-11-13 Advanced Cell Diagnostics Inc Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer
US7544754B2 (en) 2005-09-30 2009-06-09 3M Innovative Properties Company Crosslinked polymers with amine binding groups
US7544756B2 (en) * 2005-09-30 2009-06-09 3M Innovative Properties Company Crosslinked polymers with amine binding groups
WO2007044427A2 (en) * 2005-10-05 2007-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
WO2007058898A2 (en) 2005-11-10 2007-05-24 Panomics, Inc. Detection of nucleic acids through amplification of surrogate nucleic acids
JP2008043332A (ja) 2006-08-17 2008-02-28 Panomics Inc 組織スライドからの核酸定量
US7492312B2 (en) * 2006-11-14 2009-02-17 Fam Adly T Multiplicative mismatched filters for optimum range sidelobe suppression in barker code reception
US8080372B2 (en) * 2007-04-11 2011-12-20 Canon Kabushiki Kaisha Method for detecting nucleic acid in sample, method for designing probes, system for designing probes therefor
EP3136103B1 (de) 2007-10-05 2018-08-29 Affymetrix, Inc. Stark gebündelte assays
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US20090298709A1 (en) * 2008-05-28 2009-12-03 Affymetrix, Inc. Assays for determining telomere length and repeated sequence copy number
TWI419974B (zh) 2008-10-27 2013-12-21 Qiagen Gaithersburg Inc 於自動平台上之快速結果雜交捕捉法
ES2644516T3 (es) * 2009-01-28 2017-11-29 Qiagen Gaithersburg, Inc. Método y ensayo de preparación de muestras de gran volumen específico de secuencia
WO2010127228A1 (en) 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of rna splice-forms in a sample
JP5826752B2 (ja) 2009-09-14 2015-12-02 キアジェン ゲイサーズバーグ インコーポレイテッド 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法
WO2011038403A1 (en) 2009-09-28 2011-03-31 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
CA2787924A1 (en) * 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
EP2572001A2 (de) 2010-05-19 2013-03-27 QIAGEN Gaithersburg, Inc. Verfahren und zusammensetzungen für sequenzspezifische reinigung und multiplex-analyse von nukleinsäuren
US8658361B2 (en) 2010-10-21 2014-02-25 Advanced Cell Diagnostics, Inc. Ultra sensitive method for in situ detection of nucleic acids
WO2012116220A2 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
AT512416B1 (de) 2012-02-13 2013-10-15 Greiner Bio One Gmbh Anordnung und verfahren zum nachweis von mikroorganismen in einem kulturgefäss
US9783841B2 (en) 2012-10-04 2017-10-10 The Board Of Trustees Of The Leland Stanford Junior University Detection of target nucleic acids in a cellular sample
US9273349B2 (en) 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
JP6734011B2 (ja) * 2014-06-06 2020-08-05 古河電気工業株式会社 生体分子検出用試験キット、及びこれを用いた生体分子の検出方法、並びにこれらに用いられる生体分子検出用試験片及び生体分子検出用標識試薬
CN104569383A (zh) * 2015-01-13 2015-04-29 科蒂亚(新乡)生物技术有限公司 一种在聚乙烯微孔板上包被核酸的方法
EP3362462B1 (de) 2015-10-12 2021-07-21 Advanced Cell Diagnostics, Inc. In-situ-detektion von nukleotidvarianten bei proben mit hohem rauschen sowie zugehörige zusammensetzungen und verfahren
USD825184S1 (en) 2017-02-22 2018-08-14 Yeti Coolers, Llc Bag
US11076666B2 (en) 2017-03-08 2021-08-03 Yeti Coolers, Llc Container with magnetic closure
US10954055B2 (en) 2017-03-08 2021-03-23 Yeti Coolers, Llc Container with magnetic closure
DK3592173T3 (da) 2017-03-08 2024-09-23 Yeti Coolers Llc Beholder med magnetisk lukning
USD909063S1 (en) 2019-03-08 2021-02-02 Yeti Coolers, Llc Bag
WO2022076500A1 (en) 2020-10-08 2022-04-14 Ecolab Usa Inc. System and technique for detecting cleaning chemical usage to control cleaning efficacy
US11992104B2 (en) 2022-02-16 2024-05-28 Yeti Coolers, Llc Container with resealable closure
CN121613098B (zh) * 2026-02-02 2026-04-21 南昌大学 一种基于夹心法的聚苯乙烯微塑料快速检测方法及试剂盒

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0134307B1 (de) * 1983-07-29 1989-04-19 Henning Berlin GmbH Chemie und Pharmawerk Voraktivierte Kunststoffoberflächen zur Immobilisierung von organisch-chemischen und biologischen Materialien, Verfahren zur Herstellung und Verwendung derselben
US4748111A (en) * 1984-03-12 1988-05-31 Molecular Diagnostics, Inc. Nucleic acid-protein conjugate used in immunoassay
US4657853A (en) * 1984-09-14 1987-04-14 E. I. Du Pont De Nemours And Company Immunoassays utilizing covalent conjugates of polymerized enzyme and antibody
DE3524451A1 (de) * 1985-07-09 1987-03-12 Behringwerke Ag Mit ueberzuegen versehene formkoerper zur bindung von bioaffinen substanzen, verfahren zu ihrer herstellung sowie ihre verwendung
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
CA1339351C (en) * 1987-10-15 1997-08-26 Michael S. Urdea Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
CA2031659A1 (en) * 1990-01-26 1991-07-27 John B. Findlay Water-insoluble reagent, nucleic acid probe, test kit and diagnostic and purification methods
ES2116977T3 (es) * 1990-05-11 1998-08-01 Microprobe Corp Soportes solidos para ensayos de hibridacion de acidos nucleicos y metodos para inmovilizar oligonucleotidos de modo covalente.

Also Published As

Publication number Publication date
US5712383A (en) 1998-01-27
DE69230864D1 (de) 2000-05-04
EP0620864A1 (de) 1994-10-26
EP0620864B1 (de) 2000-03-29
US5747244A (en) 1998-05-05
JP3515781B2 (ja) 2004-04-05
JPH07502656A (ja) 1995-03-23
DE69230864T2 (de) 2000-11-09
CA2125145A1 (en) 1993-07-08
JP2004004087A (ja) 2004-01-08
WO1993013224A1 (en) 1993-07-08
AU3427693A (en) 1993-07-28

Similar Documents

Publication Publication Date Title
ATE191237T1 (de) Verfahren zur immobilisierung von nukleinsäuresonden an polystyroloberflächen
Gershoni et al. Protein blotting: principles and applications
ATE88762T1 (de) Methoden und zusammensetzungen fuer tests mit nukleinsaeuresonden.
ATE54028T1 (de) Hybridisierungstest fuer nukleinsaeure mittels anwendung von nachweisbaren antihybridantik¯rpern.
ATE66695T1 (de) Verfahren zur erhoehung der empfindlichkeit von nukleinsaeure-hybridisierungstesten.
DE3480487D1 (en) Nucleic acid hybridization assay
DE60040794D1 (de) Verfahren zur Immobilisierung von Oligonukleotiden auf festem Trägermaterial
ATE114334T1 (de) Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe.
DE68928853D1 (de) Befestigung von sequenzspezifischen proben
DE69434066D1 (de) Verfahren zur immobilisierung von nukleinsäure-molekülen
ATE154132T1 (de) Anionenaustauschtrennung von nukleinsäuren
ATE321879T1 (de) Verfahren und strukturen, in welchen chemisch markierte nichtradioaktive polynukleotidesonden benutzt werden
ES8900235A1 (es) Un procedimiento para preparar un estuche destinado a un ensayo de hibridacion sobre una muestra
ATE114726T1 (de) Verfahren zum schnellen nachweis von nukleinsäuren.
EP1231470A4 (de) Verfahren zum immobilisieren von substanzen
EP0351950A3 (de) Verwendung von Plasma zur Immobilisierung von Proteinen auf Polymeroberflächen
DE69825896D1 (de) Verfahren zur kovalenten immobilisierung von biomolekülen an einem träger mittels eines his-tags
DK0623177T3 (da) Tørret nucleinsyrehybridiseringsprobe bundet reversibelt til en fast bærer, fremgangsmåde, hvorved der anvendes en sådan probe, og kit egnet dertil
DE68925457D1 (de) Diagnoseverfahren für infektionskrankheiten sowie verfahren zum nachweis und identifizierung von mikroorganismen
DE3783399D1 (de) Verfahren fuer biologische reaktionen mittels an traegern immobilisierten zellen und vorrichtung zur durchfuehrung des verfahrens.
ATE118823T1 (de) Schnelles verfahren zur identifizierung einer spezifischen sequenz von nukleinsäure.
IL73578A (en) Method and reagent for the detection of polynucleotide sequence in sample of dna or rna by using a nucleic acid probe and contact of duplexes with immobilizing antibody
ATE98701T1 (de) Verfahren zur durchfuehrung von hybridisierungstests von nukleinsaeuren.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties