KR970707275A - 지질분해 활성을 갖는 효소(an enzyme with lipolytic activity) - Google Patents

지질분해 활성을 갖는 효소(an enzyme with lipolytic activity)

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Publication number
KR970707275A
KR970707275A KR1019970702748A KR19970702748A KR970707275A KR 970707275 A KR970707275 A KR 970707275A KR 1019970702748 A KR1019970702748 A KR 1019970702748A KR 19970702748 A KR19970702748 A KR 19970702748A KR 970707275 A KR970707275 A KR 970707275A
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KR
South Korea
Prior art keywords
dna sequence
enzyme
seq
dna
sequence encoding
Prior art date
Application number
KR1019970702748A
Other languages
English (en)
Inventor
토마스 샌달
사카리 카우피넨
랜 벤크 코포드
Original Assignee
안네 제케르
노보 노르디스크 아크티에 셀스카브
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Publication date
Application filed by 안네 제케르, 노보 노르디스크 아크티에 셀스카브 filed Critical 안네 제케르
Publication of KR970707275A publication Critical patent/KR970707275A/ko

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase

Abstract

본 발명은 특히 지질분해활성을 나타내는 효소를 암호화하는 DNA 서열로 구성된 DNA 구조체에 관한 것인데 이것은 a) 서열 1에 제시된 DNA 서열 또는 b) 서열 1에 제시된 DNA 서열의 유사체로 구성되며 이 유사체는 ⅰ) 서열 1에 제시된 DNA 서열과 상동하며 그리고/또는 ⅱ) 서열 1에 제시된 DNA 서열과 같은 올리고뉴클레오티드 프로브와 혼성화하며 그리고/또는 ⅲ) 서열 1에 제시된 서열로 구성된 DNA 서열에 의해 암호화되는 폴리펩티드와 상동한 폴리펩티드를 암호화하며 그리고/또는 ⅳ) 서열 1에 제시된 DNA 서열에 의해 암호화되거나 Humicola insolens DSM 1800으로부터 유래되는 정제된 지질분해 효소에 대해 발생하는 항체와 면역학적으로 반응하는 폴리펩티드를 암호화한다. 또한 이런 DNA 구조체에 의해 암호화되는 지질분해 효소와 이런 지질분해 효소로 구성된 효소제제, 세제 첨가제 및 세제 조성물이 기재되어 있다.

Description

지질분해 활성을 갖는 효소(AN ENZYME WITH LIPOLYTIC ACTIVITY)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 pH의 함수로서 본 발명에 따른 효소의 활성을 나타낸 것이다.

Claims (17)

  1. 지질분해활성을 나타내는 효소를 암호화하는 DNA 서열로 구성된 DNA 구조체에 있어서, DNA 서열이 a) 서열1에 제시된 DNA 서열 및/또는 Saccharomyces cerevisiae DSH 9975의 플라스미드로부터 얻을 수 있는 지질분해 효소를 암호화하는 DNA 서열 또는, b) 서열 1에 제시된 DNA 서열의 유사체 및/또는 Saccharomyces cerevisiae DSH 9975의 플라스미드로부터 얻을 수 있는 지질분해 효소를 암호화하는 DNA 서열의 유사체이며, 유사체가 ⅰ) 서열 1에 제시된 DNA 서열 및/또는 Saccharomyces cerevisiae DSH 9975의 플라스미드로부터 얻을 수 있는 지질분해 효소를 암호화하는 DNA 서열과 상동하며, 그리고/또는 ⅱ) 서열 1에 제시된 DNA 서열 및/또는 Saccharomyces cerevisiae DSH 9975의 플라스미드로부터 얻을 수 있는 지질분해 효소를 암호화하는 DNA 서열과 같은 올리고뉴클레오티드 프로브와 혼성화하며, 그리고/또는 ⅲ) 서열 1에 제시된 DNA 서열로 구성된 DNA 서열 및/또는 Saccharomyces cerevisiae DSH 9975의 플라스미드로부터 얻을 수 있는 지질분해 효소를 암호화하는 DNA 서열에 의해 암호화되는 폴리펩티드와 상동한 폴리펩티드를 암호화하며, 그리고/또는 ⅳ) 서열 1에 제시된 DNA 서열 및/또는 Saccharomyces cerevisiae DSH 9975의 플라스미드로부터 얻을 수 있고, 그리고/또는 Humicola insolens DSM 1800으로부터 유래되는 지질분해 효소를 암호화하는 DNA 서열에 의해 암호화되는 정제된 지질분해 효소에 대해 발생하는 항체와 면역학적으로 반응하는 폴리펩티드를 암호화하는 것을 특징으로 하는 DNA 구조체.
  2. 제1항에 있어서, 지질분해 활성을 나타내는 효소를 암호화하는 DNA 서열이 미생물로부터 얻어질 수 있는 것을 특징으로 하는 DNA 구조체.
  3. 제2항에 있어서, DNA 서열이 사상균 또는 효모로부터 얻어질 수 있는 것을 특징으로 하는 DNA 구조체.
  4. 제3항에 있어서, DNA 서열이 Thielavia Chaetomium, Gelasinospora, Neurospora, Podospora, Sodaria 또는 Humicola속 균주로부터 얻어질 수 있는 것을 특징으로 하는 DNA 구조체.
  5. 제4항에 있어서, DNA 서열이 Chaetomium 또는 Humicola속 균주 특히 H. insalens 균주로부터 얻어질 수 있는 것을 특징으로 하는 DNA 구조체.
  6. 제5항에 있어서, DNA 서열이 H. insolens DSM 1800의 DNA 라이브러리를 기초로 하여 분리되거나 생산되는 것을 특징으로 하는 DNA 구조체.
  7. 제1항 내지 제6항 중 어느 한 항에 따른 DNA 구조체로 구성된 재조합 발현 벡터.
  8. 제1항 내지 제6항 중 어느 한 항에 따른 DNA 구조체 또는 제7항에 따른 재조합 발현벡터로 구성되어 있는 세포.
  9. 제8항에 있어서, 진핵세포, 특히 효모세포 또는 사상균 세포같은 균세포인 것을 특징으로 하는 세포.
  10. 제9항에 있어서, Aspergillus 또는 Trichoderma속 균주, 특히 Aspergillus oryzae 또는 Aspergillus niger 균주에 속하는 것을 특징으로 하는 세포.
  11. 지질분해 활성을 나타내는 효소를 제조하는 방법에 있어서, 효소의 제조를 유도하는 조건에서 제8항 내지 제10항 중 어느 한 한에 따른 세포를 배양하고 그 배양물로부터 효소를 회수하는 거으로 구성된 것을 특징으로 하는 방법.
  12. 지질분해 활성을 나타내는 효소에 있어서, a) 제1항 내지 제6항 중 어느 한 항에 따른 DNA 구조체에 의해 암호화되며 그리고/또는 b) 제11항에 따른 방법에 의해 제조되며 그리고/또는 c) 서열1에 제시된 DNA 서열 및/또는 Saccharomyces cerevisiae DSM 9975의 플라스미드로부터 얻을 수 있고 그리고/또는 Humicola insolens DAM 1800으로부터 유래되는 지질분해 효소를 암호화하는 DNA 서열에 의해 암호화되는 정제된 지질 분해 효소에 대해 발생하는 항체와 면역학적으로 반응하는 것을 특징으로 하는 효소.
  13. 제12항에 따른 지질분해 효소로 구성된 효소제제.
  14. 제12항에 따른 지질분해 효소로 구성된 세제 첨가물.
  15. 제12항에 따른 지질분해 효소로 구성된 세제 조성물.
  16. 제15항에 있어서, 세탁세척용인 것을 특징으로 하는 세제 조성물.
  17. 제15항에 있어서, 식기세척용인 것을 특징으로 하는 세제 조성물.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019970702748A 1994-10-26 1995-10-26 지질분해 활성을 갖는 효소(an enzyme with lipolytic activity) KR970707275A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK124094 1994-10-26
DK1240/94 1994-10-26
PCT/DK1995/000427 WO1996013580A1 (en) 1994-10-26 1995-10-26 An enzyme with lipolytic activity

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KR970707275A true KR970707275A (ko) 1997-12-01

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Country Link
US (1) US5827719A (ko)
EP (1) EP0785994A1 (ko)
JP (1) JPH10507642A (ko)
KR (1) KR970707275A (ko)
CN (1) CN1167503A (ko)
AU (1) AU3697995A (ko)
BR (1) BR9509525A (ko)
CA (1) CA2203398A1 (ko)
FI (1) FI971749A (ko)
WO (1) WO1996013580A1 (ko)

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FI971749A0 (fi) 1997-04-24
AU3697995A (en) 1996-05-23
WO1996013580A1 (en) 1996-05-09
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BR9509525A (pt) 1995-10-26

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