DK2816112T3 - TAL effector-mediated DNA modification - Google Patents

TAL effector-mediated DNA modification Download PDF

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Publication number
DK2816112T3
DK2816112T3 DK14183521.5T DK14183521T DK2816112T3 DK 2816112 T3 DK2816112 T3 DK 2816112T3 DK 14183521 T DK14183521 T DK 14183521T DK 2816112 T3 DK2816112 T3 DK 2816112T3
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ala
leu
val
gin
pro
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DK14183521.5T
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Danish (da)
English (en)
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Daniel Voytas
Feng Zhang
Tomas Cermak
Clarice Lauer Schmidt
Erin Doyle
Adam J Bogdanove
Michelle Christian
Li Wang
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Univ Minnesota
Univ Iowa State Res Found Inc
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43825298&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK2816112(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Minnesota, Univ Iowa State Res Found Inc filed Critical Univ Minnesota
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    • A61K38/46—Hydrolases (3)
    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
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    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
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    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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DK14183521.5T 2009-12-10 2010-12-10 TAL effector-mediated DNA modification DK2816112T3 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US28532409P 2009-12-10 2009-12-10
US35210810P 2010-06-07 2010-06-07
US36668510P 2010-07-22 2010-07-22
EP10799163.0A EP2510096B2 (en) 2009-12-10 2010-12-10 Tal effector-mediated dna modification

Publications (1)

Publication Number Publication Date
DK2816112T3 true DK2816112T3 (en) 2018-11-19

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ID=43825298

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Application Number Title Priority Date Filing Date
DK14183521.5T DK2816112T3 (en) 2009-12-10 2010-12-10 TAL effector-mediated DNA modification
DK10799163.0T DK2510096T4 (en) 2009-12-10 2010-12-10 NUMBER EFFECTOR-MEDIATED DNA MODIFICATION

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Application Number Title Priority Date Filing Date
DK10799163.0T DK2510096T4 (en) 2009-12-10 2010-12-10 NUMBER EFFECTOR-MEDIATED DNA MODIFICATION

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US (10) US8586363B2 (OSRAM)
EP (3) EP3456826B1 (OSRAM)
JP (3) JP2013513389A (OSRAM)
KR (2) KR102110725B1 (OSRAM)
CN (2) CN102770539B (OSRAM)
AU (2) AU2010327998B2 (OSRAM)
CA (1) CA2783351C (OSRAM)
DK (2) DK2816112T3 (OSRAM)
ES (2) ES2696825T3 (OSRAM)
HU (1) HUE041436T2 (OSRAM)
IL (2) IL220234B (OSRAM)
NO (1) NO2510096T3 (OSRAM)
PL (2) PL2816112T3 (OSRAM)
PT (2) PT2816112T (OSRAM)
SG (1) SG181601A1 (OSRAM)
TR (1) TR201815882T4 (OSRAM)
WO (1) WO2011072246A2 (OSRAM)

Families Citing this family (848)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
EP2449135B1 (en) * 2009-06-30 2016-01-06 Sangamo BioSciences, Inc. Rapid screening of biologically active nucleases and isolation of nuclease-modified cells
NO2510096T3 (OSRAM) 2009-12-10 2015-03-21
US20110203012A1 (en) * 2010-01-21 2011-08-18 Dotson Stanton B Methods and compositions for use of directed recombination in plant breeding
WO2011100058A1 (en) * 2010-02-09 2011-08-18 Sangamo Biosciences, Inc. Targeted genomic modification with partially single-stranded donor molecules
EP2563918B1 (en) * 2010-04-26 2016-11-23 Sangamo BioSciences, Inc. Genome editing of a rosa locus using zinc-finger nucleases
CN103025344B (zh) 2010-05-17 2016-06-29 桑格摩生物科学股份有限公司 新型dna-结合蛋白及其用途
BR122021005283B1 (pt) 2010-06-02 2022-02-22 Evolva, Inc Hospedeiro recombinante que compreende genes recombinantes para produção deesteviol ou glicosídeo de esteviol, método para produzir esteviol, glicosídeo de esteviol ou composição de glicosídeo de esteviol e método para sintetizar esteviol ou glicosídeo de esteviol
EP2392208B1 (en) * 2010-06-07 2016-05-04 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Fusion proteins comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease and their use
EP2580331A4 (en) * 2010-06-14 2013-11-27 Univ Iowa State Res Found Inc NUCLEASE ACTIVITY OF THE TAL EFFECTOR AND FUSION PROTEIN FOKI
US8945868B2 (en) 2010-07-21 2015-02-03 Sangamo Biosciences, Inc. Methods and compositions for modification of a HLA locus
US20240397918A1 (en) * 2011-02-25 2024-12-05 Recombinetics, Inc. Non-meiotic allele introgression
US10920242B2 (en) 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
US9528124B2 (en) 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
CA2828239C (en) * 2011-02-25 2020-10-06 Recombinetics, Inc. Genetically modified animals and methods for making the same
KR101982360B1 (ko) * 2011-04-05 2019-05-24 셀렉티스 콤팩트 tale-뉴클레아제의 발생 방법 및 이의 용도
JP6158170B2 (ja) 2011-04-27 2017-07-12 アミリス， インコーポレイテッド ゲノム修飾のための方法
TR201802544T4 (tr) 2011-06-06 2018-03-21 Bayer Cropscience Nv Önceden seçilmiş bir bölgede bir bitki genomunu modifiye için yöntemler ve araçlar.
CA2841710C (en) * 2011-07-15 2021-03-16 The General Hospital Corporation Methods of transcription activator like effector assembly
EP2734621B1 (en) 2011-07-22 2019-09-04 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9161995B2 (en) 2011-07-25 2015-10-20 Sangamo Biosciences, Inc. Methods and compositions for alteration of a cystic fibrosis transmembrane conductance regulator (CFTR) gene
US9370551B2 (en) 2011-07-27 2016-06-21 The Broad Institute, Inc. Compositions and methods of treating head and neck cancer
EP2737066B1 (en) * 2011-07-29 2017-11-08 Cellectis High throughput method for assembly and cloning polynucleotides comprising highly similar polynucleotidic modules
WO2013019745A1 (en) * 2011-07-29 2013-02-07 Georgia Health Sciences University Methods and compositions for genetically modifiying cells
CN102787125B (zh) * 2011-08-05 2013-12-04 北京大学 一种构建tale重复序列的方法
BR122021015509B1 (pt) 2011-08-08 2022-03-29 Evolva Sa Método para produzir um glicosídeo de esteviol alvo
BR122014004140B8 (pt) 2011-08-22 2023-03-28 Bayer Cropscience Ag Vetor recombinante ou construção recombinante, bem como métodos para obter e produzir uma planta de algodão ou célula vegetal tolerante a um inibidor de hppd, e para cultivar um campo de plantas de algodão
EP3498833B1 (en) 2011-09-21 2023-08-16 Sangamo Therapeutics, Inc. Methods and compositions for regulation of transgene expression
WO2013050611A1 (en) 2011-10-07 2013-04-11 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2013050593A1 (en) 2011-10-07 2013-04-11 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2013050318A1 (en) 2011-10-07 2013-04-11 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2013053711A1 (en) 2011-10-10 2013-04-18 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
WO2013053686A1 (en) 2011-10-10 2013-04-18 Basf Plant Science Company Gmbh Method of producing plants having increased resistance to pathogens
BR112014008895A2 (pt) 2011-10-12 2019-09-24 Bayer Cropscience Ag plantas com atividade diminuída de uma enzima de desfosforilação do amido
BR112014008723A2 (pt) 2011-10-12 2019-09-24 Bayer Cropscience Ag plantas com diminuição da atividade de uma enzima de desforilação de àmido
US9513283B2 (en) 2011-10-21 2016-12-06 Kyushu University, National University Corporation Method for designing RNA binding protein utilizing PPR motif, and use thereof
CA2852955C (en) 2011-10-27 2021-02-16 Sangamo Biosciences, Inc. Methods and compositions for modification of the hprt locus
MX2014005212A (es) 2011-10-31 2014-11-25 Pioneer Hi Bred Int Mejoramiento de la tolerancia a la sequia, eficiencia de uso de nitrogeno y rendimiento de una planta.
CA2854819C (en) 2011-11-16 2022-07-19 Sangamo Biosciences, Inc. Modified dna-binding proteins and uses thereof
US8450107B1 (en) 2011-11-30 2013-05-28 The Broad Institute Inc. Nucleotide-specific recognition sequences for designer TAL effectors
US20130137173A1 (en) * 2011-11-30 2013-05-30 Feng Zhang Nucleotide-specific recognition sequences for designer tal effectors
US10801017B2 (en) 2011-11-30 2020-10-13 The Broad Institute, Inc. Nucleotide-specific recognition sequences for designer TAL effectors
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
MX354995B (es) 2011-12-05 2018-03-27 Factor Bioscience Inc Metodos y productos para transfeccion de celulas.
BR112014014105B1 (pt) 2011-12-16 2023-10-10 Targetgene Biotechnologies Ltd Método para modificar um sítio-alvo predeterminado no interior de um ácido nucleico genômico ou organelar alvo em uma célula hospedeira eucariótica por um complexo núcleoproteína
DE102011122267A1 (de) 2011-12-23 2013-06-27 Kws Saat Ag Neue aus Pflanzen stammende cis-regulatorische Elemente für die Entwicklung Pathogen-responsiver chimärer Promotoren
US9688997B2 (en) 2011-12-29 2017-06-27 Iowa State University Research Foundation, Inc. Genetically modified plants with resistance to Xanthomonas and other bacterial plant pathogens
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
EP2612918A1 (en) 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
WO2013136274A1 (en) 2012-03-13 2013-09-19 University Of Guelph Myb55 promoter and use thereof
CA2865531A1 (en) 2012-03-13 2013-09-19 University Of Guelph Methods of increasing tolerance to heat stress and amino acid content of plants
DK2825190T3 (en) * 2012-03-15 2017-02-27 Cellectis REPEAT VARIABLE DIRESTS FOR TARGETING NUCLEOTIDES
SG11201405956SA (en) 2012-03-23 2014-11-27 Cellectis Method to overcome dna chemical modifications sensitivity of engineered tale dna binding domains
CN102628037B (zh) * 2012-03-31 2013-07-24 西南大学 家蚕油蚕基因BmBlos2遗传改造系统及其制备方法和应用
US20130274129A1 (en) * 2012-04-04 2013-10-17 Geneart Ag Tal-effector assembly platform, customized services, kits and assays
WO2013158309A2 (en) 2012-04-18 2013-10-24 The Board Of Trustees Of The Leland Stanford Junior University Non-disruptive gene targeting
EP2841581B2 (en) 2012-04-23 2023-03-08 BASF Agricultural Solutions Seed US LLC Targeted genome engineering in plants
SG11201406547YA (en) 2012-04-25 2014-11-27 Regeneron Pharma Nuclease-mediated targeting with large targeting vectors
EP2841572B1 (en) 2012-04-27 2019-06-19 Duke University Genetic correction of mutated genes
JP6352250B2 (ja) 2012-05-02 2018-07-04 ダウ アグロサイエンシィズ エルエルシー リンゴ酸デヒドロゲナーゼの標的改変
CA2871524C (en) * 2012-05-07 2021-07-27 Sangamo Biosciences, Inc. Methods and compositions for nuclease-mediated targeted integration of transgenes
US11120889B2 (en) 2012-05-09 2021-09-14 Georgia Tech Research Corporation Method for synthesizing a nuclease with reduced off-site cleavage
US9834783B2 (en) 2012-05-25 2017-12-05 Eg Crop Science, Inc. Dirigent gene EG261 and its orthologs and paralogs and their uses for pathogen resistance in plants
DK3473707T3 (da) * 2012-05-25 2025-03-17 Cellectis Fremgangsmåder til engineering af allogen og immunsuppressiv resistent t-celle til immunoterapi
SI3401400T1 (sl) 2012-05-25 2019-10-30 Univ California Postopki in sestavki za RNA usmerjeno modifikacijo tarčne DNA in za RNA usmerjeno modulacijo prepisovanja
US9605274B2 (en) 2012-05-25 2017-03-28 Evolutionary Genomics, Inc. Dirigent gene EG261 and its orthologs and paralogs and their uses for pathogen resistance in plants
US9890364B2 (en) 2012-05-29 2018-02-13 The General Hospital Corporation TAL-Tet1 fusion proteins and methods of use thereof
WO2013182910A2 (en) * 2012-06-05 2013-12-12 Cellectis New transcription activator-like effector (tale) fusion protein
CA2875945A1 (en) 2012-06-07 2013-12-12 The Children's Hospital Of Philadelphia Controlled gene expression methods
EP2858486A4 (en) 2012-06-12 2016-04-13 Hoffmann La Roche METHOD AND COMPOSITIONS FOR GENERATING ALLELES WITH CONDITIONAL KNOCKOUT
BR112014031260A2 (pt) 2012-06-15 2019-08-20 Du Pont métodos e composições que envolvem variantes de als com preferência de substrato nativo
BR112014031891A2 (pt) 2012-06-19 2017-08-01 Univ Minnesota direcionamento genético nas plantas utilizando vírus de dna
AR091482A1 (es) * 2012-06-21 2015-02-04 Recombinetics Inc Celulas modificadas geneticamente y metodos par su obtencion
WO2013191769A1 (en) * 2012-06-22 2013-12-27 Mayo Foundation For Medical Education And Research Genome editing
AU2013285840A1 (en) 2012-07-06 2015-02-05 National Institute Of Agrobiological Sciences Wheat with increased grain-bearing number and method for producing same, and chemical for increasing grain-bearing number of wheat
CA2875618C (en) 2012-07-11 2021-04-27 Sangamo Biosciences, Inc. Methods and compositions for the treatment of lysosomal storage diseases
US20150104873A1 (en) * 2012-07-11 2015-04-16 University of Nevada, Las Vegas Genome Surgery with Paired, Permeant Endonuclease Construct
US10883119B2 (en) 2012-07-11 2021-01-05 Sangamo Therapeutics, Inc. Methods and compositions for delivery of biologics
EP2687605A1 (en) 2012-07-19 2014-01-22 Biogemma Method for performing homologous recombination
US20140230083A1 (en) 2012-07-24 2014-08-14 Cellectis Modular base-specific nucleic acid binding domains from burkholderia rhizoxinica proteins
CN105188767A (zh) * 2012-07-25 2015-12-23 布罗德研究所有限公司 可诱导的dna结合蛋白和基因组干扰工具及其应用
US10058078B2 (en) 2012-07-31 2018-08-28 Recombinetics, Inc. Production of FMDV-resistant livestock by allele substitution
SG11201500852WA (en) 2012-08-29 2015-04-29 Sangamo Biosciences Inc Methods and compositions for treatment of a genetic condition
EP2893016B1 (en) * 2012-09-03 2019-06-19 Cellectis Methods for modulating tal specificity
JP6442406B2 (ja) 2012-09-04 2018-12-19 ザ スクリプス リサーチ インスティテュート 標的結合特異性を有するキメラポリペプチド
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
HK1217732A1 (zh) 2012-09-07 2017-01-20 美国陶氏益农公司 Fad3性能基因座及相應的能夠誘導靶向斷裂的靶位點特異性結合蛋白
EP2895620B1 (en) 2012-09-11 2017-08-02 Life Technologies Corporation Nucleic acid amplification
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
EP3789405A1 (en) 2012-10-12 2021-03-10 The General Hospital Corporation Transcription activator-like effector (tale) - lysine-specific demethylase 1 (lsd1) fusion proteins
EP2914714A4 (en) * 2012-10-30 2016-09-21 Recombinetics Inc CONTROLLING GENDER TREATMENT IN ANIMALS
US20150291967A1 (en) 2012-10-31 2015-10-15 Luc Mathis Coupling herbicide resistance with targeted insertion of transgenes in plants
KR102315098B1 (ko) 2012-11-01 2021-10-21 팩터 바이오사이언스 인크. 세포에서 단백질을 발현시키는 방법들과 생성물들
US20140120578A1 (en) 2012-11-01 2014-05-01 Medicago Inc. Plants for production of therapeutic proteins
EP2922959A4 (en) * 2012-11-20 2016-04-20 Simplot Co J R TAL-MEDIATED TRANSFER DNA INTRODUCTION
EP2929017A4 (en) 2012-12-05 2016-09-28 Sangamo Biosciences Inc METHOD AND COMPOSITIONS FOR REGULATING METABOLISM DISEASES
EP3138911B1 (en) 2012-12-06 2018-12-05 Sigma Aldrich Co. LLC Crispr-based genome modification and regulation
WO2014093701A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
BR112015013784A2 (pt) 2012-12-12 2017-07-11 Massachusetts Inst Technology aplicação, manipulação e otimização de sistemas, métodos e composições para manipulação de sequência e aplicações terapêuticas
CN103013954B (zh) * 2012-12-17 2014-03-05 中国科学院遗传与发育生物学研究所 水稻基因badh2的定点敲除系统及其应用
US9708589B2 (en) 2012-12-18 2017-07-18 Monsanto Technology Llc Compositions and methods for custom site-specific DNA recombinases
EP2934097B1 (en) * 2012-12-21 2018-05-02 Cellectis Potatoes with reduced cold-induced sweetening
WO2014100525A2 (en) 2012-12-21 2014-06-26 Pioneer Hi-Bred International, Inc. Compositions and methods for auxin-analog conjugation
CN105051195B (zh) 2013-02-06 2019-09-06 埃沃尔瓦公司 用于提高莱鲍迪苷d和莱鲍迪苷m之产生的方法
EP2954042B1 (en) * 2013-02-07 2017-12-06 The General Hospital Corporation Tale transcriptional activators
MX2015010098A (es) 2013-02-11 2016-04-19 Evolva Sa Producción eficiente de glicosidos de esteviol en huéspedes recombinantes.
CN103319574A (zh) * 2013-02-16 2013-09-25 清华大学 分离的多肽及其应用
CN109913495B (zh) 2013-02-20 2022-11-25 瑞泽恩制药公司 大鼠的遗传修饰
US20150353885A1 (en) 2013-02-21 2015-12-10 Cellectis Method to counter-select cells or organisms by linking loci to nuclease components
EP2958996B1 (en) 2013-02-25 2019-10-16 Sangamo Therapeutics, Inc. Methods and compositions for enhancing nuclease-mediated gene disruption
US9393257B2 (en) * 2013-03-01 2016-07-19 Regents Of The University Of Minnesota TALEN-based gene correction
CN105473721A (zh) 2013-03-11 2016-04-06 先锋国际良种公司 采用磺酰脲类依赖型稳定化结构域的方法和组合物
BR112015023286A2 (pt) 2013-03-14 2018-03-06 Arzeda Corp polipeptídeo recombinante com atividade da dicamba descarboxilase, construto de polinucleotídeo, célula, método de produção de uma célula hospedeira compreendendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade da dicamba descarboxilase, método para descarboxilar dicamba, um derivado de dicamba ou um metabolito de dicamba, método para a detecção de um polipeptideo e método para a detecção da presença de um polinucleotideo que codifica um polipeptideo tendo atividade da dicamba descarboxilase
US20140315985A1 (en) 2013-03-14 2014-10-23 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
EP2970995A1 (en) 2013-03-14 2016-01-20 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
CN105246324B (zh) * 2013-03-15 2018-08-28 塞尔克蒂斯股份有限公司 通过靶向敲除fad2-1a/1b基因来改良大豆油成分
WO2014144155A1 (en) * 2013-03-15 2014-09-18 Regents Of The University Of Minnesota Engineering plant genomes using crispr/cas systems
US11039586B2 (en) 2013-03-15 2021-06-22 Monsanto Technology Llc Creation and transmission of megaloci
US10113162B2 (en) 2013-03-15 2018-10-30 Cellectis Modifying soybean oil composition through targeted knockout of the FAD2-1A/1B genes
WO2014144094A1 (en) * 2013-03-15 2014-09-18 J.R. Simplot Company Tal-mediated transfer dna insertion
US10793867B2 (en) 2013-03-15 2020-10-06 Monsanto Technology, Llc Methods for targeted transgene-integration using custom site-specific DNA recombinases
US9828582B2 (en) 2013-03-19 2017-11-28 Duke University Compositions and methods for the induction and tuning of gene expression
CN105121650A (zh) 2013-04-02 2015-12-02 拜尔作物科学公司 真核生物中的靶向基因组工程
UA121197C2 (uk) 2013-04-05 2020-04-27 Доу Агросайенсіс Ллс Нуклеаза "цинкові пальці" для модифікацїї гена ahas та спосіб її використання
CN105008536A (zh) * 2013-04-16 2015-10-28 深圳华大基因科技服务有限公司 分离的寡核苷酸及其用途
SG10201808935WA (en) 2013-04-16 2018-11-29 Regeneron Pharma Targeted modification of rat genome
SG11201508730TA (en) 2013-04-22 2015-11-27 Univ Kyushu Nat Univ Corp Dna-binding protein using ppr motif, and use thereof
EP2796558A1 (en) 2013-04-23 2014-10-29 Rheinische Friedrich-Wilhelms-Universität Bonn Improved gene targeting and nucleic acid carrier molecule, in particular for use in plants
CN103233004B (zh) * 2013-04-28 2015-04-29 新疆农垦科学院 一种人工dna分子及检测目标基因表达的方法
WO2014186435A2 (en) 2013-05-14 2014-11-20 University Of Georgia Research Foundation, Inc. Compositions and methods for reducing neointima formation
CN105531372A (zh) 2013-06-14 2016-04-27 塞尔克蒂斯股份有限公司 植物中非转基因基因组编辑方法
EP3011033B1 (en) 2013-06-17 2020-02-19 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof
EP3011030B1 (en) 2013-06-17 2023-11-08 The Broad Institute, Inc. Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation
DK3011031T3 (da) 2013-06-17 2020-12-21 Broad Inst Inc Fremføring og anvendelse af crispr-cas-systemerne, vektorer og sammensætninger til levermålretning og -terapi
EP2818867A1 (en) 2013-06-27 2014-12-31 INSERM (Institut National de la Santé et de la Recherche Médicale) Antibodies conjugated to at least one nucleic acid molecule and their use in multiplex immuno-detection assays
US20160369268A1 (en) * 2013-07-01 2016-12-22 The Board Of Regents Of The University Of Texas System Transcription activator-like effector (tale) libraries and methods of synthesis and use
US10563225B2 (en) 2013-07-26 2020-02-18 President And Fellows Of Harvard College Genome engineering
CA2920253A1 (en) 2013-08-02 2015-02-05 Enevolv, Inc. Processes and host cells for genome, pathway, and biomolecular engineering
US10006011B2 (en) 2013-08-09 2018-06-26 Hiroshima University Polypeptide containing DNA-binding domain
JP5931022B2 (ja) 2013-08-09 2016-06-08 国立大学法人広島大学 Ｄｎａ結合ドメインを含むポリペプチド
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
SI3041345T1 (sl) 2013-09-04 2024-08-30 KWS SAAT SE & Co. KGaA Rastlina, odporna na helminthosporium turcicum
DE102013014637A1 (de) 2013-09-04 2015-03-05 Kws Saat Ag HELMlNTHOSPORlUM TURClCUM-RESlSTENTE PFLANZE
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
IL312865B2 (en) 2013-09-11 2025-06-01 Eagle Biologics Inc Liquid protein formulations containing viscosity-lowering agents
US10526616B2 (en) * 2013-09-23 2020-01-07 Rensselaer Polytechnic Institute Nanoparticle-mediated gene delivery, genomic editing and ligand-targeted modification in various cell populations
CN105874062B (zh) 2013-10-18 2021-07-20 先锋国际良种公司 草甘膦-n-乙酰转移酶(glyat)序列以及使用方法
MX388563B (es) 2013-10-25 2025-03-20 Livestock Improvement Corporation Ltd Marcadores geneticos y usos de los mismos.
US10449254B2 (en) 2013-11-03 2019-10-22 The Regents Of The University Of California Ionic liquids for transdermal drug delivery
EP3862434A1 (en) 2013-11-04 2021-08-11 Dow AgroSciences LLC Optimal soybean loci
UA120503C2 (uk) 2013-11-04 2019-12-26 Дау Агросайєнсиз Елелсі Спосіб одержання трансгенної клітини рослини кукурудзи
MX362066B (es) 2013-11-04 2019-01-07 Dow Agrosciences Llc Loci óptimos de soya.
UY35812A (es) 2013-11-04 2015-05-29 Dow Agrosciences Llc ?loci de maíz óptimos?.
EP3067419B1 (en) 2013-11-06 2021-03-17 Hiroshima University Vector for nucleic acid insertion
WO2015070062A1 (en) * 2013-11-07 2015-05-14 Massachusetts Institute Of Technology Cell-based genomic recorded accumulative memory
EP2878667A1 (en) 2013-11-29 2015-06-03 Institut Pasteur TAL effector means useful for partial or full deletion of DNA tandem repeats
CN103710360A (zh) * 2013-12-03 2014-04-09 南方医科大学珠江医院 提高HepGL肝癌细胞氨基甲酰磷酸合成酶表达的方法
SMT201800653T1 (it) 2013-12-11 2019-01-11 Regeneron Pharma Metodi e composizioni per la modificazione indirizzata a bersaglio di un genoma
JP6174811B2 (ja) 2013-12-11 2017-08-02 リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． ゲノムの標的改変のための方法及び組成物
IL289736B2 (en) 2013-12-12 2025-09-01 Massachusetts Inst Technology Administration, use and therapeutic applications of CRISPR–Cas gene editing systems and gene editing preparations
KR20160089530A (ko) 2013-12-12 2016-07-27 더 브로드 인스티튜트, 인코퍼레이티드 Ｈｂｖ 및 바이러스 질병 및 질환을 위한 ｃｒｉｓｐｒ­ｃａｓ 시스템 및 조성물의 전달，용도 및 치료적 적용
WO2015089351A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders
WO2015089486A2 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems
WO2015089473A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
US20160333063A1 (en) 2013-12-13 2016-11-17 The General Hospital Corporation Soluble high molecular weight (hmw) tau species and applications thereof
WO2015092460A1 (en) 2013-12-18 2015-06-25 INSERM (Institut National de la Santé et de la Recherche Médicale) New gene mutations for the diagnosis of arthrogryposis multiplex congenita and congenital peripheral neuropathies disease
AU2014368982B2 (en) 2013-12-19 2021-03-25 Amyris, Inc. Methods for genomic integration
US10233456B2 (en) * 2014-01-30 2019-03-19 The Board Of Trustees Of The University Of Arkansas Method, vectors, cells, seeds and kits for stacking genes into a single genomic site
RU2714404C2 (ru) 2014-01-31 2020-02-14 Фэктор Байосайенс Инк. Способы и продукты для получения и доставки нуклеиновых кислот
DK3102673T3 (da) 2014-02-03 2020-07-06 Sangamo Therapeutics Inc Fremgangsmåder og sammensætninger til behandling af beta-talassæmi
CN104844696A (zh) * 2014-02-19 2015-08-19 北京大学 一种转录激活子样效应因子功能蛋白设计、合成及其应用
TW201538518A (zh) 2014-02-28 2015-10-16 Dow Agrosciences Llc 藉由嵌合基因調控元件所賦予之根部特異性表現
SG11201609211VA (en) * 2014-03-05 2016-12-29 Nat Univ Corp Univ Kobe Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
MX392639B (es) 2014-03-21 2025-03-24 Univ Leland Stanford Junior Vectores virales recombinantes para la integración de transgenes
JP6815986B2 (ja) 2014-03-26 2021-01-20 ユニバーシティ オブ メリーランド， カレッジ パーク 大型家畜の接合体における標的化ゲノム編集
WO2015153889A2 (en) * 2014-04-02 2015-10-08 University Of Florida Research Foundation, Incorporated Materials and methods for the treatment of latent viral infection
US9522936B2 (en) 2014-04-24 2016-12-20 Sangamo Biosciences, Inc. Engineered transcription activator like effector (TALE) proteins
BR112016024945A2 (pt) 2014-04-28 2017-10-24 Recombinetics Inc edição de gene multiplex em suínos
CN104611365B (zh) * 2014-07-17 2017-06-16 清华大学 利用tale转录抑制子在哺乳动物细胞中模块化构建合成基因线路
WO2015165275A1 (zh) * 2014-04-30 2015-11-05 清华大学 利用tale转录抑制子在哺乳动物细胞中模块化构建合成基因线路
WO2015165276A1 (zh) * 2014-04-30 2015-11-05 清华大学 利用tale转录抑制子在哺乳动物细胞中模块化构建合成基因线路的试剂盒
DE102014106327A1 (de) 2014-05-07 2015-11-12 Universitätsklinikum Hamburg-Eppendorf (UKE) TAL-Effektornuklease zum gezielten Knockout des HIV-Korezeptors CCR5
WO2015172149A1 (en) 2014-05-09 2015-11-12 Yale University Hyperbranched polyglycerol-coated particles and methods of making and using thereof
US11918695B2 (en) 2014-05-09 2024-03-05 Yale University Topical formulation of hyperbranched polymer-coated particles
WO2015175642A2 (en) 2014-05-13 2015-11-19 Sangamo Biosciences, Inc. Methods and compositions for prevention or treatment of a disease
MX385689B (es) 2014-06-06 2025-03-18 Regeneron Pharma Métodos y composiciones para modificar un locus dirigido.
WO2015188870A1 (en) 2014-06-12 2015-12-17 Sesvanderhave N.V. Use of selectable marker gene in sugar beet protoplasts transformation method and system
US20170121723A1 (en) 2014-06-12 2017-05-04 Sesvanderhave N.V. Transformation method of sugar beet protoplasts by talen platform technology
EP3158072B1 (en) 2014-06-20 2021-01-13 Cellectis Potatoes with reduced granule-bound starch synthase
US9738897B2 (en) 2014-06-23 2017-08-22 Regeneron Pharmaceuticals, Inc. Nuclease-mediated DNA assembly
WO2015200805A2 (en) 2014-06-26 2015-12-30 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modifications and methods of use
WO2016000237A1 (en) 2014-07-03 2016-01-07 Pioneer Overseas Corporation Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
BR112017002783A2 (pt) 2014-08-11 2017-12-19 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
CN107429241B (zh) 2014-08-14 2025-10-24 百奥赛图(北京)医药科技股份有限公司 Dna敲入系统
CN105367628B (zh) * 2014-08-19 2019-07-26 深圳华大基因农业控股有限公司 一对高效编辑水稻waxy基因的talen其识别打靶位点及其应用
CN105367631B (zh) * 2014-08-25 2019-05-14 深圳华大基因科技有限公司 一种转录激活子样效应因子核酸酶及其编码基因和应用
IL292575A (en) 2014-09-07 2022-06-01 Selecta Biosciences Inc Methods and preparations for weakening immune responses by modulating gene expression in an antiviral transfer system
US10612064B2 (en) 2014-09-09 2020-04-07 Evolva Sa Production of steviol glycosides in recombinant hosts
KR101528812B1 (ko) * 2014-09-18 2015-06-15 강원대학교산학협력단 SETDB1 HMTase 발현 저해방법
CN105440111B (zh) * 2014-09-30 2019-08-13 深圳华大基因研究院 一对转录激活子样效应因子核酸酶及其编码序列与应用
MX391067B (es) 2014-10-01 2025-03-21 Eagle Biologics Inc Formulaciones de polisacáridos y ácido nucleico que contienen agentes de reducción de viscosidad.
CN104357440B (zh) * 2014-10-09 2017-06-13 中山大学 一对靶向斑马鱼Forkhead box n1基因的Talen识别序列及其mRNA制备方法
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
EP3561052A1 (en) 2014-10-15 2019-10-30 Regeneron Pharmaceuticals, Inc. Methods and compositions for generating or maintaining pluripotent cells
WO2016066671A1 (en) 2014-10-29 2016-05-06 INSERM (Institut National de la Santé et de la Recherche Médicale) Method for treating resistant cancers using progastrin inhibitors
PT3216867T (pt) 2014-11-04 2020-07-16 Univ Kobe Nat Univ Corp Método para modificar a sequência de genoma para introduzir mutação específica a sequência de adn alvo por reação de remoção de bases, e complexo molecular nele utilizado
CN107532142A (zh) * 2014-11-11 2018-01-02 应用干细胞有限公司 使用同源重组改造间充质干细胞
WO2016077429A1 (en) 2014-11-12 2016-05-19 Recombinetics, Inc. Heterozygous modifications of tumor suppressor genes and swine model of neurofibromatosis type 1
DE102014016667B4 (de) 2014-11-12 2024-03-07 Kws Saat Se Haploideninduktoren
US20160138040A1 (en) * 2014-11-13 2016-05-19 Cellectis Brassica engineered to confer herbicide tolerance
EP3985115A1 (en) 2014-12-12 2022-04-20 The Broad Institute, Inc. Protected guide rnas (pgrnas)
US20170327834A1 (en) 2014-12-15 2017-11-16 Syngenta Participations Ag Pesticidal microrna carriers and use thereof
MX388784B (es) 2014-12-19 2025-03-20 Regeneron Pharma Métodos y composiciones para la modificación genética dirigida a través de direccionamiento múltiple de una sola etapa.
CA2969151A1 (en) 2014-12-23 2016-06-30 Syngenta Participations Ag Methods and compositions for identifying and enriching for cells comprising site specific genomic modifications
EP3702456A1 (en) 2014-12-24 2020-09-02 The Broad Institute, Inc. Crispr having or associated with destabilization domains
WO2016118560A1 (en) * 2015-01-20 2016-07-28 Mayo Foundation For Medical Education And Research Methods and materials for assembling nucleic acid constructs
CN114058582A (zh) 2015-01-26 2022-02-18 菲特治疗公司 用于诱导造血细胞分化的方法和组合物
CA2973674A1 (en) 2015-01-30 2016-08-04 Evolva Sa Production of steviol glycosides in recombinant hosts
CN107709551A (zh) 2015-02-02 2018-02-16 塞尔克蒂斯股份有限公司 无t‑dna整合的农杆菌介导的基因组修饰
WO2016124918A1 (en) 2015-02-03 2016-08-11 The Institute Of Genetics And Developmental Biology Plants with increased seed size
WO2016124920A1 (en) 2015-02-03 2016-08-11 The Institute Of Genetics And Developmental Biology Rice plants with altered seed phenotype and quality
WO2016130600A2 (en) 2015-02-09 2016-08-18 Duke University Compositions and methods for epigenome editing
US20180031579A1 (en) 2015-02-12 2018-02-01 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for predicting the responsiveness of a patient affected with malignant hematological disease to chemotherapy treatment and methods of treatment of such disease
CA2976376A1 (en) 2015-02-13 2016-08-18 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
JP6590333B2 (ja) * 2015-02-26 2019-10-16 学校法人東京理科大学 Ｄｎａ結合ドメイン組込み用ベクターおよびそのセット、融合タンパク質コーディングベクターおよびそのセットならびにその製造方法、デスティネーションベクター、植物細胞用発現ベクターおよびその製造方法、植物細胞用発現ベクター作製用キット、形質転換方法、ならびにゲノム編集方法
US11261466B2 (en) 2015-03-02 2022-03-01 Sinai Health System Homologous recombination factors
WO2016142427A1 (en) 2015-03-10 2016-09-15 INSERM (Institut National de la Santé et de la Recherche Médicale) Method ank kit for reprogramming somatic cells
US10604743B2 (en) 2015-03-16 2020-03-31 Dsm Ip Assets B.V. UDP-glycosyltransferases
WO2016155482A1 (zh) * 2015-03-16 2016-10-06 中国科学院遗传与发育生物学研究所 一种利用非遗传物质对植物基因组进行定点改造的方法
WO2016153305A1 (ko) * 2015-03-26 2016-09-29 한국생명공학연구원 표적 유전자 특이적 핵산 프로브 및 Fok Ι 제한효소 이량체를 이용하여 세포 내에서 표적 유전자를 특이적으로 편집하기 위한 조성물 및 이의 용도
US11046959B2 (en) * 2015-03-30 2021-06-29 The Board Of Regents Of The Nevada System Of Higher Education On Behalf Of The University Of Nevada, Las Vegas Compositions comprising TALENs and methods of treating HIV
AU2016243052C1 (en) 2015-04-03 2022-11-24 Dana-Farber Cancer Institute, Inc. Composition and methods of genome editing of B-cells
PT3289080T (pt) 2015-04-30 2021-11-19 Univ Columbia Terapia genética para doenças autossómicas dominantes
WO2016178207A1 (en) 2015-05-04 2016-11-10 Ramot At Tel-Aviv University Ltd. Methods and kits for fragmenting dna
EP3095870A1 (en) 2015-05-19 2016-11-23 Kws Saat Se Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom
WO2016205759A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
TWI813532B (zh) 2015-06-18 2023-09-01 美商博得學院股份有限公司 降低脱靶效應的ｃｒｉｓｐｒ酶突變
ES2594486B1 (es) 2015-06-19 2017-09-26 Biopraxis Research Aie Molécula de ácido nucleico, proteína de fusión y método para modificar el material genético de una célula
US11193131B2 (en) 2015-06-30 2021-12-07 Regents Of The University Of Minnesota Haploid inducer line for accelerated genome editing
AU2016295291A1 (en) 2015-07-17 2018-02-08 Institute Of Genetics And Developmental Biology Chinese Academy Of Sciences Wheat plants resistant to powdery mildew
US20190024073A1 (en) * 2015-07-23 2019-01-24 Mayo Foundation For Medical Education And Research Editing mitochondrial dna
CA2995067A1 (en) 2015-08-07 2017-02-16 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3337908A4 (en) 2015-08-18 2019-01-23 The Broad Institute, Inc. METHOD AND COMPOSITIONS FOR CHANGING THE FUNCTION AND STRUCTURE OF CHROMATIN GRINDING AND / OR DOMAINS
CA2995582A1 (en) * 2015-08-19 2017-02-23 Children's Research Institute, Children's National Medical Center Compositions and methods for treating graft versus host disease
US10767173B2 (en) 2015-09-09 2020-09-08 National University Corporation Kobe University Method for converting genome sequence of gram-positive bacterium by specifically converting nucleic acid base of targeted DNA sequence, and molecular complex used in same
US10837024B2 (en) 2015-09-17 2020-11-17 Cellectis Modifying messenger RNA stability in plant transformations
WO2017066497A2 (en) 2015-10-13 2017-04-20 Duke University Genome engineering with type i crispr systems in eukaryotic cells
US11207393B2 (en) 2015-10-16 2021-12-28 President And Fellows Of Harvard College Regulatory T cell PD-1 modulation for regulating T cell effector immune responses
IL258821B (en) 2015-10-23 2022-07-01 Harvard College Nucleobase editors and their uses
WO2017075261A1 (en) 2015-10-27 2017-05-04 Recombinetics, Inc. Engineering of humanized car t-cells and platelets by genetic complementation
EP3368157B1 (en) 2015-10-29 2022-06-29 Dana-Farber Cancer Institute, Inc. Methods for identification, assessment, prevention, and treatment of metabolic disorders using pm20d1 and n-lipidated amino acids
WO2017079428A1 (en) 2015-11-04 2017-05-11 President And Fellows Of Harvard College Site specific germline modification
ES2953925T3 (es) 2015-11-04 2023-11-17 Fate Therapeutics Inc Ingeniería genómica de células pluripotentes
CN115927199A (zh) 2015-11-04 2023-04-07 菲特治疗公司 用于诱导造血细胞分化的方法和组合物
US11001622B2 (en) 2015-11-19 2021-05-11 The Brigham And Women's Hospital, Inc. Method of treating autoimmune disease with lymphocyte antigen CD5-like (CD5L) protein
US11220693B2 (en) 2015-11-27 2022-01-11 National University Corporation Kobe University Method for converting monocot plant genome sequence in which nucleic acid base in targeted DNA sequence is specifically converted, and molecular complex used therein
ES2899176T3 (es) 2015-11-27 2022-03-10 Kws Saat Se & Co Kgaa Plantas tolerantes a las bajas temperaturas
AU2016362282B2 (en) 2015-11-30 2023-03-16 Duke University Therapeutic targets for the correction of the human dystrophin gene by gene editing and methods of use
AU2016369490C1 (en) 2015-12-18 2021-12-23 Sangamo Therapeutics, Inc. Targeted disruption of the T cell receptor
JP2018537112A (ja) 2015-12-18 2018-12-20 サンガモ セラピューティクス， インコーポレイテッド Ｍｈｃ細胞受容体の標的化破壊
DE102015016445A1 (de) 2015-12-21 2017-06-22 Kws Saat Se Restorer-Pflanze
US11840693B2 (en) 2015-12-21 2023-12-12 KWS SAAT SE & Co. KGaA Restorer plants
DE102015017161A1 (de) 2015-12-21 2017-06-22 Kws Saat Se Restorer-Pflanze
EP3901258A1 (en) 2016-01-11 2021-10-27 The Board of Trustees of the Leland Stanford Junior University Chimeric proteins and methods of immunotherapy
NZ743983A (en) 2016-01-11 2025-08-29 Univ Leland Stanford Junior Chimeric proteins and methods of regulating gene expression
EP4646936A3 (en) 2016-02-02 2025-11-19 Cellectis Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes
EP3416976A2 (en) 2016-02-16 2018-12-26 Yale University Compositions for enhancing targeted gene editing and methods of use thereof
WO2017143061A1 (en) 2016-02-16 2017-08-24 Yale University Compositions and methods for treatment of cystic fibrosis
CN116763942A (zh) 2016-02-18 2023-09-19 宾州研究基金会 脑内生成gaba能神经元
EP3417061B1 (en) 2016-02-18 2022-10-26 The Regents of the University of California Methods and compositions for gene editing in stem cells
US20190032088A1 (en) 2016-02-26 2019-01-31 Cellectis Micelle based system nuclease encapsulation for in-vivo gene editing
WO2023150553A1 (en) 2022-02-01 2023-08-10 University Of Rochester Gpr17 promoter-based targeting and transduction of glial progenitor cells
EP3429567B1 (en) 2016-03-16 2024-01-10 The J. David Gladstone Institutes Methods and compositions for treating obesity and/or diabetes and for identifying candidate treatment agents
KR20190080825A (ko) 2016-03-21 2019-07-08 다나-파버 캔서 인스티튜트 인크. T-세포 기능소실 상태-특이적 유전자 발현 조절인자 및 그 용도
AU2017237912B2 (en) 2016-03-23 2022-03-10 The Regents Of The University Of California Methods of treating mitochondrial disorders
US20190117799A1 (en) 2016-04-01 2019-04-25 The Brigham And Women's Hospital, Inc. Stimuli-responsive nanoparticles for biomedical applications
US20190127713A1 (en) 2016-04-13 2019-05-02 Duke University Crispr/cas9-based repressors for silencing gene targets in vivo and methods of use
WO2017180711A1 (en) 2016-04-13 2017-10-19 Editas Medicine, Inc. Grna fusion molecules, gene editing systems, and methods of use thereof
SG11201808856TA (en) 2016-04-13 2018-11-29 Evolva Sa Production of steviol glycosides in recombinant hosts
AU2017250295B2 (en) * 2016-04-14 2022-08-25 Fred Hutchinson Cancer Center Compositions and methods to program therapeutic cells using targeted nucleic acid nanocarriers
US10188749B2 (en) 2016-04-14 2019-01-29 Fred Hutchinson Cancer Research Center Compositions and methods to program therapeutic cells using targeted nucleic acid nanocarriers
EP3429633B1 (en) 2016-04-15 2021-02-24 Cellectis A method of engineering drug-specific hypersensitive t-cells for immunotherapy by gene inactivation
CN107312788B (zh) * 2016-04-26 2020-07-28 中国科学院动物研究所 一种tale重复序列载体的构建方法
US11730826B2 (en) 2016-05-02 2023-08-22 Massachusetts Institute Of Technology Amphiphilic nanoparticles for delivery of CRISPR based therapy
CN109312378A (zh) 2016-05-16 2019-02-05 埃沃尔瓦公司 在重组宿主中产生甜菊醇糖苷
EP4219731A3 (en) 2016-05-18 2023-08-09 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
CN105950623B (zh) * 2016-05-19 2018-11-30 电子科技大学 一种用于talen高效构建的双rvd单元模块库及talen构建方法
CN109476715B (zh) 2016-05-26 2023-06-27 纽海姆有限公司 产无籽果实的植物
US11293021B1 (en) 2016-06-23 2022-04-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
EP3474849B1 (en) 2016-06-27 2025-05-21 The Broad Institute, Inc. Compositions and methods for detecting and treating diabetes
US20190359992A1 (en) 2016-06-28 2019-11-28 Cellectis Altering expression of gene products in plants through targeted insertion of nucleic acid sequences
CN105949293B (zh) * 2016-06-28 2019-08-13 福建农林大学 植物源高效转录激活功能域sac3及应用
WO2018007263A1 (en) 2016-07-06 2018-01-11 Cellectis Sequential gene editing in primary immune cells
EP4321623A3 (en) 2016-07-15 2024-05-15 Salk Institute for Biological Studies Methods and compositions for genome editing in non-dividing cells
EP4275747A3 (en) 2016-07-19 2024-01-24 Duke University Therapeutic applications of cpf1-based genome editing
US10548302B2 (en) 2016-07-29 2020-02-04 Regeneron Pharmaceuticals, Inc. Fibrillin-1 mutations for modeling neonatal progeroid syndrome with congenital lipodystrophy
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
EP4056031A1 (en) 2016-08-11 2022-09-14 The Jackson Laboratory Methods and compositions relating to improved human red blood cell survival in genetically modified immunodeficient non-human animals
US10576167B2 (en) 2016-08-17 2020-03-03 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
UY40582A (es) 2016-08-17 2024-01-15 Monsanto Technology Llc MÉTODOS, COMPOSICIONES y PLANTAS MEDIANTE LA MANIPULACIÓN DEL METABOLISMO DE GIBERELINA PARA AUMENTAR EL RENDIMIENTO COSECHABLE
CA3034271A1 (en) 2016-08-18 2018-02-22 Cellectis Black-spot resistant potatoes with reduced tuber-specific polyphenol oxidase activity
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
IL264792B2 (en) 2016-08-24 2023-10-01 Sangamo Therapeutics Inc Engineered target-specific zinc-finger nucleases
KR102552848B1 (ko) 2016-08-29 2023-07-06 더 리전츠 오브 더 유니버시티 오브 캘리포니아 피부 치료를 위한 이온성 약제에 기초한 국소 제형
CA3035484A1 (en) 2016-09-01 2018-03-08 Cellectis Methods for altering amino acid content in plants
US10960085B2 (en) 2016-09-07 2021-03-30 Sangamo Therapeutics, Inc. Modulation of liver genes
JP7466308B2 (ja) 2016-09-20 2024-04-12 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド Ｕｓｐ１０バイオマーカーおよびモジュレーターを用いたａｍｌの同定、評価、予防および処置のための組成物および方法
US20180084767A1 (en) 2016-09-21 2018-03-29 Recombinetics, Inc. Animal models for cardiomyopathy
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
US12499971B2 (en) 2016-09-28 2025-12-16 The Broad Institute, Inc. Systematic screening and mapping of regulatory elements in non-coding genomic regions, methods, compositions, and applications thereof
JP6887492B6 (ja) 2016-10-04 2021-07-14 プレシジョン バイオサイエンシズ，インク． 遺伝子改変細胞で使用するための共刺激ドメイン
EP3523319B1 (en) 2016-10-10 2023-06-28 Limagrain Europe Nucleic acid encoding sm1 resistance to orange wheat blossom midge and method of use
WO2018069232A1 (en) 2016-10-10 2018-04-19 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for predicting the risk of having cardiac hypertrophy
JP7588390B2 (ja) 2016-10-14 2024-11-22 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸塩基エディターのａａｖ送達
GB201617559D0 (en) 2016-10-17 2016-11-30 University Court Of The University Of Edinburgh The Swine comprising modified cd163 and associated methods
WO2018073391A1 (en) 2016-10-19 2018-04-26 Cellectis Targeted gene insertion for improved immune cells therapy
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
WO2018081081A1 (en) 2016-10-24 2018-05-03 Javier Gil Humanes Multiplex gene targeting in plants
WO2018076335A1 (en) 2016-10-31 2018-05-03 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Compositions and methods for enhancing abiotic stress tolerance
CN109906030B (zh) 2016-11-04 2022-03-18 安健基因公司 用于产生仅重链抗体的经基因修饰的非人动物和方法
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts
US12227578B2 (en) 2016-11-11 2025-02-18 The Broad Institute, Inc. Modulation of intestinal epithelial cell differentiation, maintenance and/or function through T cell action
CA3042857A1 (en) 2016-11-16 2018-05-24 Cellectis Methods for altering amino acid content in plants through frameshift mutations
JP6948718B2 (ja) 2016-11-28 2021-10-13 国立大学法人大阪大学 ゲノム編集方法
US10519456B2 (en) 2016-12-02 2019-12-31 Syngenta Participations Ag Simultaneous gene editing and haploid induction
CA3045131A1 (en) 2016-12-14 2018-06-21 Ligandal, Inc. Methods and compositions for nucleic acid and protein payload delivery
US20200085758A1 (en) 2016-12-16 2020-03-19 The Brigham And Women's Hospital, Inc. Co-delivery of nucleic acids for simultaneous suppression and expression of target genes
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
WO2018123938A1 (ja) * 2016-12-27 2018-07-05 国立研究開発法人農業・食品産業技術総合研究機構 ゲノム編集植物の作出方法
CN110121336A (zh) 2017-01-05 2019-08-13 弗莱德哈钦森癌症研究中心 改善疫苗功效的系统和方法
GB201700380D0 (en) 2017-01-10 2017-02-22 Plant Bioscience Ltd Methods of increasing seed yield
RU2019126483A (ru) 2017-01-23 2021-02-24 Ридженерон Фармасьютикалз, Инк. Варианты 17-бета-гидроксистероиддегидрогеназы 13 (hsd17b13) и их применение
US12344826B2 (en) 2017-01-25 2025-07-01 The George Washington University, A Congressionally Chartered Not-For-Profit Corporation Apparatus and methods for in vitro preclinical human trials
WO2018146481A1 (en) 2017-02-09 2018-08-16 Fujian Agriculture And Forestry University Expression of a phosphate transporter for improving plant yield
JP2020508056A (ja) 2017-02-22 2020-03-19 クリスパー・セラピューティクス・アクチェンゲゼルシャフトＣＲＩＳＰＲ Ｔｈｅｒａｐｅｕｔｉｃｓ ＡＧ 遺伝子編集のための組成物および方法
EP3366778A1 (de) 2017-02-28 2018-08-29 Kws Saat Se Haploidisierung in sorghum
BR112019018200B1 (pt) 2017-03-03 2024-02-20 Pioneer Hi-Bred International, Inc Método para medir a quantidade de um sucrosil-oligossacarídeo,método para medir estaquiose, método para processar sementes de soja geneticamente modificadas
CN110662556A (zh) 2017-03-09 2020-01-07 哈佛大学的校长及成员们 癌症疫苗
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
JP7623785B2 (ja) 2017-03-21 2025-01-29 ザ ジャクソン ラボラトリー ヒトＡＰＯＥ４およびマウスＴｒｅｍ２ ｐ．Ｒ４７Ｈを発現する遺伝子改変されたマウス、ならびにその使用の方法
CA3057432C (en) 2017-03-22 2023-08-08 National University Corporation Kobe University Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted dna using cell endogenous dna modifying enzyme, and molecular complex used therein
BR112019019655A2 (pt) 2017-03-23 2020-04-22 Harvard College editores de nucleobase que compreendem proteínas de ligação a dna programáveis por ácido nucleico
US10980779B2 (en) 2017-03-24 2021-04-20 Inserm (Institut National De La Sante Et De La Recherche Medicale) GFI1 inhibitors for the treatment of hyperglycemia
CA3057759A1 (en) 2017-03-24 2018-09-27 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Methods for increasing grain yield
US12161694B2 (en) 2017-03-24 2024-12-10 The Broad Institute, Inc. Methods and compositions for regulating innate lymphoid cell inflammatory responses
US11542497B2 (en) 2017-03-30 2023-01-03 The University Of Tokyo Method for evaluating multiple different genes of interest
US11963966B2 (en) 2017-03-31 2024-04-23 Dana-Farber Cancer Institute, Inc. Compositions and methods for treating ovarian tumors
US20200113821A1 (en) 2017-04-04 2020-04-16 Yale University Compositions and methods for in utero delivery
US20200071773A1 (en) 2017-04-12 2020-03-05 Massachusetts Eye And Ear Infirmary Tumor signature for metastasis, compositions of matter methods of use thereof
WO2018191520A1 (en) 2017-04-12 2018-10-18 The Broad Institute, Inc. Respiratory and sweat gland ionocytes
EP3392339A1 (en) 2017-04-18 2018-10-24 Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen Improved genome editing in plant cells
CN111031790A (zh) 2017-04-20 2020-04-17 E开创生物技术股份有限公司 产生基因修改的动物的方法
WO2018195486A1 (en) 2017-04-21 2018-10-25 The Broad Institute, Inc. Targeted delivery to beta cells
HUE067206T2 (hu) 2017-04-25 2024-10-28 Cellectis Csökkent ligninösszetételû lucerna
ES2981244T3 (es) 2017-04-28 2024-10-07 Acuitas Therapeutics Inc Novedosas formulaciones de nanopartículas de lípidos de carbonilo y lípidos para la administración de ácidos nucleicos
CA3059793A1 (en) 2017-05-03 2018-11-08 Sangamo Therapeutics, Inc. Methods and compositions for modification of a cystic fibrosis transmembrane conductance regulator (cftr) gene
WO2018204777A2 (en) 2017-05-05 2018-11-08 The Broad Institute, Inc. Methods for identification and modification of lncrna associated with target genotypes and phenotypes
WO2018208837A1 (en) 2017-05-08 2018-11-15 Precision Biosciences, Inc. Nucleic acid molecules encoding an engineered antigen receptor and an inhibitory nucleic acid molecule and methods of use thereof
WO2018209324A2 (en) 2017-05-11 2018-11-15 The Broad Institute, Inc. Methods and compositions of use of cd8+ tumor infiltrating lymphocyte subtypes and gene signatures thereof
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
RU2019140867A (ru) 2017-05-12 2021-06-15 Зе Джексон Лаборатори Nsg мыши, не имеющие mhc класса i и класса ii
WO2018211032A1 (en) 2017-05-17 2018-11-22 Evolva Sa Production of steviol glycosides in recombinant hosts
CA3064817A1 (en) 2017-05-25 2018-11-29 Institute Of Genetics And Developmental Biology Chinese Academy Of Sciences Methods for increasing grain productivity
EP3409104A1 (en) 2017-05-31 2018-12-05 Vilmorin et Cie Tomato plant resistant to tomato yellow leaf curl virus, powdery mildew, and nematodes
EP3409106A1 (en) 2017-06-01 2018-12-05 Vilmorin et Cie Tolerance in plants of solanum lycopersicum to the tobamovirus tomato brown rugose fruit virus (tbrfv)
WO2020249996A1 (en) 2019-06-14 2020-12-17 Vilmorin & Cie Resistance in plants of solanum lycopersicum to the tobamovirus tomato brown rugose fruit virus
US11879128B2 (en) 2017-06-05 2024-01-23 Consejo Superior De Investigaciones Científicas (Csic)—Delegación Andalucía Targeting of gluten by genome editing
CN110997906B (zh) 2017-06-05 2024-05-07 雷杰纳荣制药公司 B4galt1变体及其用途
WO2018226685A2 (en) 2017-06-06 2018-12-13 Dana-Farber Cancer Institute, Inc. Methods for sensitizing cancer cells to t cell-mediated killing by modulating molecular pathways
WO2018231999A1 (en) 2017-06-13 2018-12-20 Regents Of The University Of Minnesota Materials and methods for increasing gene editing frequency
US11897953B2 (en) 2017-06-14 2024-02-13 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
US20200362355A1 (en) 2017-06-15 2020-11-19 The Regents Of The University Of California Targeted non-viral dna insertions
US11512287B2 (en) 2017-06-16 2022-11-29 Sangamo Therapeutics, Inc. Targeted disruption of T cell and/or HLA receptors
US20210139870A1 (en) 2017-06-19 2021-05-13 Cellectis Anti-hbv combination therapies involving specific endonucleases
PL3538645T3 (pl) 2017-06-20 2021-11-08 Institut Curie Komórki odpornościowe z defektem suv39h1
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
CA3067842C (en) 2017-06-23 2025-11-04 University Of Kentucky Research Foundation METHODS FOR MODULATING THE ALKALOID CONTENT OF A PLANT OR PART OF THAT PLANT
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
CA3064601A1 (en) 2017-06-26 2019-01-03 The Broad Institute, Inc. Crispr/cas-adenine deaminase based compositions, systems, and methods for targeted nucleic acid editing
WO2019005957A1 (en) 2017-06-30 2019-01-03 Precision Biosciences, Inc. GENETICALLY MODIFIED T CELLS COMPRISING A MODIFIED INTRON IN THE ALPHA T CELL RECEPTOR GENE
CA3066253C (en) 2017-06-30 2023-10-31 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
CN111094573A (zh) * 2017-07-12 2020-05-01 梅约医学教育与研究基金会 有效靶向敲入或基因置换的材料和方法
US12049643B2 (en) 2017-07-14 2024-07-30 The Broad Institute, Inc. Methods and compositions for modulating cytotoxic lymphocyte activity
US12105089B2 (en) 2017-07-17 2024-10-01 The Broad Institute, Inc. Cell atlas of the healthy and ulcerative colitis human colon
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
CN111278848B (zh) * 2017-08-04 2023-06-27 北京大学 特异性识别甲基化修饰dna碱基的tale rvd及其应用
US20190045761A1 (en) 2017-08-11 2019-02-14 Recombinetics, Inc. Inducible disease models methods of making them and use in tissue complementation
CN111263810A (zh) 2017-08-22 2020-06-09 纳匹基因公司 使用多核苷酸指导的核酸内切酶的细胞器基因组修饰
EP3447134B1 (en) 2017-08-22 2023-10-11 KWS SAAT SE & Co. KGaA Increased fungal resistance in crop plants
WO2019038417A1 (en) 2017-08-25 2019-02-28 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences METHODS FOR INCREASING GRAIN YIELD
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
HRP20250980T1 (hr) 2017-08-29 2025-10-24 KWS SAAT SE & Co. KGaA Unaprijeđeni sistem plavog aleurona i drugi sistemi segregacije
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
BR112020005217A2 (pt) 2017-09-18 2020-09-15 Amyris, Inc. métodos para a engenharia genética de células hospedeiras de kluyveromyces
BR112020005166A2 (pt) 2017-09-18 2020-09-24 Amyris, Inc. métodos para integração genômica para células hospedeiras de kluyveromyces
WO2019060759A1 (en) 2017-09-21 2019-03-28 Dana-Farber Cancer Institute, Inc. ISOLATION, PRESERVATION, COMPOSITIONS AND USES OF PLANT EXTRACTS JUSTICIA
EP3585162B1 (en) 2017-09-29 2023-08-30 Regeneron Pharmaceuticals, Inc. Rodents comprising a humanized ttr locus and methods of use
WO2019068022A1 (en) 2017-09-30 2019-04-04 Inscripta, Inc. CONTINUOUS FLOW ELECTROPORATION INSTRUMENTATION
US20200239544A1 (en) 2017-10-03 2020-07-30 Precision Biosciences, Inc. Modified epidermal growth factor receptor peptides for use in genetically-modified cells
US20200255828A1 (en) 2017-10-04 2020-08-13 The Broad Institute, Inc. Methods and compositions for altering function and structure of chromatin loops and/or domains
AU2018347583A1 (en) 2017-10-13 2020-05-21 Selecta Biosciences, Inc. Methods and compositions for attenuating anti-viral transfer vector IgM responses
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
AU2018353112B2 (en) 2017-10-19 2024-11-14 Cellectis Targeted gene integration of NK inhibitors genes for improved immune cells therapy
IL274179B2 (en) 2017-10-27 2024-02-01 Univ California Targeted replacement of endogenous T cell receptors
CA3080454A1 (en) 2017-10-31 2019-05-09 Vilmorin & Cie Wheat comprising male fertility restorer alleles
EP3704238B1 (en) 2017-11-01 2024-01-03 Precision Biosciences, Inc. Engineered nucleases that target human and canine factor viii genes as a treatment for hemophilia a
US12018080B2 (en) 2017-11-13 2024-06-25 The Broad Institute, Inc. Methods and compositions for treating cancer by targeting the CLEC2D-KLRB1 pathway
WO2019094955A1 (en) 2017-11-13 2019-05-16 The Broad Institute, Inc. Methods and compositions for targeting developmental and oncogenic programs in h3k27m gliomas
WO2019094984A1 (en) 2017-11-13 2019-05-16 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics during adult neurogenesis in single cells
EP3713644B1 (en) 2017-11-20 2024-08-07 University of Georgia Research Foundation, Inc. Compositions and methods for modulating hif-2a to improve muscle generation and repair
WO2019106163A1 (en) 2017-12-01 2019-06-06 Cellectis Reprogramming of genetically engineered primary immune cells
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
WO2019118893A1 (en) 2017-12-15 2019-06-20 Dana-Farber Cancer Institute, Inc. Stabilized peptide-mediated targeted protein degradation
EP3728587A4 (en) 2017-12-18 2022-01-26 Syngenta Participations Ag SITES FOR TARGETED INSERTION IN THE MAIZE GENOME
ES3037765T3 (en) 2017-12-22 2025-10-07 Fate Therapeutics Inc Enhanced immune effector cells and use thereof
EP3501268B1 (en) 2017-12-22 2021-09-15 KWS SAAT SE & Co. KGaA Regeneration of plants in the presence of histone deacetylase inhibitors
CN107868123B (zh) 2017-12-25 2020-05-12 中国农业科学院作物科学研究所 一种同时提高植物产量和抗性的基因及其应用
EP3508581A1 (en) 2018-01-03 2019-07-10 Kws Saat Se Regeneration of genetically modified plants
US11994512B2 (en) 2018-01-04 2024-05-28 Massachusetts Institute Of Technology Single-cell genomic methods to generate ex vivo cell systems that recapitulate in vivo biology with improved fidelity
BR112020014168A2 (pt) 2018-01-12 2020-12-08 Basf Se Proteína, ácido nucleico isolado, gene recombinante, vetor, célula hospedeira, planta, parte de planta ou semente de trigo, métodos para produzir, produto de trigo, farinha, farelo integral, amido, grânulos de amido ou farelo de trigo e métodos para identificar e/ou selecionar uma planta de trigo
CN111818794A (zh) 2018-02-14 2020-10-23 中国科学院遗传与发育生物学研究所 增加营养物利用效率的方法
BR112020015486A2 (pt) 2018-02-15 2020-12-08 Monsanto Technology Llc Métodos melhorados para a produção de semente de milho híbrido
UY38095A (es) 2018-02-15 2019-10-01 Monsanto Technology Llc Composiciones y métodos para mejorar los rendimientos de cultivos mediante el apilamiento de rasgos
KR20200139679A (ko) 2018-02-26 2020-12-14 안톨알엑스, 인크. 면역관용성 리포솜 및 그의 사용 방법
EP3765622B1 (en) 2018-03-12 2025-04-23 Pioneer Hi-Bred International, Inc. Methods for plant transformation
EP4186921A1 (en) 2018-03-23 2023-05-31 The Trustees of Columbia University in the City of New York Gene editing for autosomal dominant diseases
WO2019189147A1 (ja) 2018-03-26 2019-10-03 国立大学法人神戸大学 細胞の有する二本鎖ｄｎａの標的部位を改変する方法
EP3545756A1 (en) 2018-03-28 2019-10-02 KWS SAAT SE & Co. KGaA Regeneration of plants in the presence of inhibitors of the histone methyltransferase ezh2
EP3749762A1 (en) 2018-03-29 2020-12-16 Cellectis Tale-nucleases for allele-specific codon modification
AU2019241967A1 (en) 2018-03-29 2020-11-19 Inscripta, Inc. Automated control of cell growth rates for induction and transformation
CA3093020A1 (en) 2018-03-29 2019-10-03 Fate Therapeutics, Inc. Engineered immune effector cells and use thereof
WO2019195738A1 (en) 2018-04-06 2019-10-10 Children's Medical Center Corporation Compositions and methods for somatic cell reprogramming and modulating imprinting
US12103972B2 (en) 2018-04-06 2024-10-01 Dana-Farber Cancer Institute, Inc. KIR3DL3 as an HHLA2 receptor, anti-HHLA2 antibodies, and uses thereof
GB201805865D0 (en) 2018-04-09 2018-05-23 Innes John Centre Genes
US10376889B1 (en) 2018-04-13 2019-08-13 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US11981892B2 (en) 2018-04-16 2024-05-14 University Of Massachusetts Compositions and methods for improved gene editing
WO2019204643A2 (en) 2018-04-18 2019-10-24 Altius Institute For Biomedical Sciences Animal pathogen-derived polypeptides and uses thereof for genetic engineering
US10508273B2 (en) 2018-04-24 2019-12-17 Inscripta, Inc. Methods for identifying selective binding pairs
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
DK3560330T3 (da) 2018-04-24 2022-07-11 Kws Saat Se & Co Kgaa Planter med forbedret fordøjelighed og markørhaplotyper
TWI686477B (zh) * 2018-04-25 2020-03-01 國立成功大學 特異性造成植物葉綠體基因變異的轉殖載體、套組、方法及利用其產生之轉殖植物細胞與農桿菌
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
WO2019210268A2 (en) 2018-04-27 2019-10-31 The Broad Institute, Inc. Sequencing-based proteomics
CA3099102C (en) 2018-05-02 2024-05-14 Cellectis Engineering wheat with increased dietary fiber
US20210386829A1 (en) 2018-05-04 2021-12-16 The Broad Institute, Inc. Compositions and methods for modulating cgrp signaling to regulate innate lymphoid cell inflammatory responses
EP3567111B1 (en) 2018-05-09 2025-07-09 KWS SAAT SE & Co. KGaA Gene for resistance to a pathogen of the genus heterodera
EP3790629A1 (en) 2018-05-11 2021-03-17 CRISPR Therapeutics AG Methods and compositions for treating cancer
GB201808424D0 (en) 2018-05-23 2018-07-11 Lucite Int Uk Ltd Methods for producing BMA and MMA using genetically modified microorganisms
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
US20210371932A1 (en) 2018-06-01 2021-12-02 Massachusetts Institute Of Technology Methods and compositions for detecting and modulating microenvironment gene signatures from the csf of metastasis patients
GB201809273D0 (en) 2018-06-06 2018-07-25 Vib Vzw Novel mutant plant cinnamoyl-coa reductase proteins
CN112424365A (zh) 2018-06-07 2021-02-26 以色列国家农业部、农村发展农业研究组织·沃尔卡尼中心 核酸构建体及其使用方法
AU2019282761B2 (en) 2018-06-07 2024-01-04 The Brigham And Women's Hospital, Inc. Methods for generating hematopoietic stem cells
IL279271B2 (en) 2018-06-07 2025-05-01 The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo Methods for Cannabis Regeneration and Transformation
US12036240B2 (en) 2018-06-14 2024-07-16 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
AU2019285082B2 (en) 2018-06-15 2024-09-19 KWS SAAT SE & Co. KGaA Methods for enhancing genome engineering efficiency
CA3103500A1 (en) 2018-06-15 2019-12-19 KWS SAAT SE & Co. KGaA Methods for improving genome engineering and regeneration in plant ii
EP3806619A1 (en) 2018-06-15 2021-04-21 Nunhems B.V. Seedless watermelon plants comprising modifications in an abc transporter gene
CN112566924B (zh) 2018-06-15 2024-10-29 科沃施种子欧洲股份两合公司 改善植物中基因组工程化和再生的方法
WO2019246483A1 (en) 2018-06-21 2019-12-26 The Jackson Laboratory Genetically modified mouse models of alzheimer's disease
WO2020006112A1 (en) 2018-06-26 2020-01-02 Regents Of The University Of Minnesota Delivery of developmental regulators to plants for the induction of meristematic tissue with genetic alterations
EP3814493A4 (en) 2018-06-27 2022-03-30 Altius Institute For Biomedical Sciences NUCLEIC ACID BINDING DOMAINS AND METHODS OF USE THEREOF
US20210324398A1 (en) 2018-06-29 2021-10-21 Pioneer Hi-Bred International, Inc. Edited nac genes in plants
CA3105331A1 (en) 2018-07-09 2020-01-16 Cellectis Canola with high oleic acid
EP3831946A4 (en) 2018-07-31 2022-08-10 The University of Tokyo METHOD OF MEASUREMENT OF MEMBRANE PROTEIN ACTIVITY
WO2020028617A1 (en) 2018-08-01 2020-02-06 University Of Georgia Research Foundation, Inc. Compositions and methods for improving embryo development
GB201812603D0 (en) 2018-08-02 2018-09-19 British American Tobacco Investments Ltd Method
EP3833758A4 (en) 2018-08-07 2022-05-18 Modalis Therapeutics Corporation NOVEL TRANSCRIPTION ACTIVATOR
EP3607819A1 (en) 2018-08-10 2020-02-12 Vilmorin et Cie Resistance to xanthomonas campestris pv. campestris (xcc) in cauliflower
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
IL292273B2 (en) 2018-08-14 2023-10-01 Inscripta Inc Devices, modules and methods for improved detection of edited sequences in living cells
WO2020041380A1 (en) 2018-08-20 2020-02-27 The Broad Institute, Inc. Methods and compositions for optochemical control of crispr-cas9
US11834686B2 (en) 2018-08-23 2023-12-05 Sangamo Therapeutics, Inc. Engineered target specific base editors
CA3108922A1 (en) 2018-08-29 2020-03-05 Amyris, Inc. Cells and methods for selection based assay
BR112021003469A2 (pt) 2018-08-30 2021-05-18 The University Of North Carolina At Chapel Hill genes sintéticos de feedback ativado, cassetes de seed match alvo e seus usos
WO2020081149A2 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
CN109706155B (zh) * 2018-08-30 2022-04-19 南京农业大学 pOsHEN1::OsSPL14基因表达盒及其构建方法和应用
AU2019328326A1 (en) 2018-08-31 2021-03-18 Yale University Compositions and methods for enhancing triplex and nuclease-based gene editing
SG11202101975RA (en) 2018-08-31 2021-03-30 Univ Yale Compositions and methods for enhancing donor oligonucleotide-based gene editing
EP3623379A1 (en) 2018-09-11 2020-03-18 KWS SAAT SE & Co. KGaA Beet necrotic yellow vein virus (bnyvv)-resistance modifying gene
EP3849565A4 (en) 2018-09-12 2022-12-28 Fred Hutchinson Cancer Research Center REDUCTION OF CD33 EXPRESSION TO SELECTIVELY PROTECT THERAPEUTIC CELLS
JP2022500052A (ja) 2018-09-18 2022-01-04 サンガモ セラピューティクス， インコーポレイテッド プログラム細胞死１（ｐｄ１）特異的ヌクレアーゼ
JP7344300B2 (ja) 2018-09-18 2023-09-13 ブイエヌブイ ニューコ インク． Ａｒｃベースのカプシドおよびその使用
KR20210060561A (ko) * 2018-09-20 2021-05-26 사노피 인트론-기반 범용 클로닝 방법 및 조성물
IL281615B1 (en) 2018-09-21 2025-09-01 Acuitas Therapeutics Inc Systems and methods for producing lipid nanoparticles and liposomes
GB201815672D0 (en) 2018-09-26 2018-11-07 Innes John Centre Methods for altering starch granule size profile
WO2020071528A1 (ja) 2018-10-04 2020-04-09 株式会社カネカ 植物のゲノム編集に用いられるｄｎａ構築物
WO2020077236A1 (en) 2018-10-12 2020-04-16 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
WO2020079033A1 (en) 2018-10-15 2020-04-23 Fondazione Telethon Genome editing methods and constructs
BR112021007306A2 (pt) 2018-10-16 2021-07-27 Pioneer Hi-Bred International, Inc. mapeamento fino por edição genômica e identificação de gene causal
WO2020081730A2 (en) 2018-10-16 2020-04-23 Massachusetts Institute Of Technology Methods and compositions for modulating microenvironment
AU2019368215B2 (en) 2018-10-22 2023-05-18 Inscripta, Inc. Engineered enzymes
KR20210082205A (ko) 2018-10-22 2021-07-02 유니버시티 오브 로체스터 레트로바이러스 인테그라제-Cas9 융합 단백질을 이용한 유도된 비상동 DNA 삽입에 의한 게놈 편집
US11214781B2 (en) 2018-10-22 2022-01-04 Inscripta, Inc. Engineered enzyme
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
EP3873540A4 (en) 2018-10-31 2022-07-27 Mayo Foundation for Medical Education and Research METHODS AND MATERIALS FOR THE TREATMENT OF CANCER
EP3873500A4 (en) 2018-10-31 2023-01-11 Mayo Foundation for Medical Education and Research METHODS AND MATERIALS FOR THE TREATMENT OF CANCER
GB201817971D0 (en) 2018-11-02 2018-12-19 British American Tobacco Investments Ltd Method
ES3015004T3 (en) 2018-11-02 2025-04-28 Annexon Inc Compositions and methods for treating brain injury
KR20210089712A (ko) 2018-11-07 2021-07-16 크리스퍼 테라퓨틱스 아게 항-cd33 면역세포 암 치료법
WO2020095248A1 (en) 2018-11-07 2020-05-14 Crispr Therapeutics Ag Anti-ptk7 immune cell cancer therapy
JP7471289B2 (ja) 2018-11-07 2024-04-19 クリスパー セラピューティクス アクチェンゲゼルシャフト 抗ｌｉｖ１免疫細胞癌療法
US12165743B2 (en) 2018-11-09 2024-12-10 The Broad Institute, Inc. Compressed sensing for screening and tissue imaging
US12402610B2 (en) 2018-11-09 2025-09-02 The Broad Institute, Inc. Methods and compositions for modulating innate lymphoid cell pathogenic effectors
GB201818715D0 (en) 2018-11-16 2019-01-02 British American Tobacco Investments Ltd Method
US12227548B2 (en) 2018-11-19 2025-02-18 Dana-Farber Cancer Institute, Inc. Use of IRE1α-XBP1 signaling pathway biomarkers for modulating immune responses
BR112021010079A2 (pt) 2018-11-27 2021-11-23 Khona Scient Llc Armações multienzimáticas bidirecionais para a biossíntese de canabinoides
WO2020112195A1 (en) 2018-11-30 2020-06-04 Yale University Compositions, technologies and methods of using plerixafor to enhance gene editing
WO2020117526A1 (en) 2018-12-02 2020-06-11 Fate Therapeutics, Inc. IMMUNOTHERAPIES USING ENHANCED iPSC DERIVED EFFECTOR CELLS
BR112021010753A2 (pt) 2018-12-05 2021-09-21 Vertex Pharmaceuticals Incorporated Sistemas de edição de genes para editar um gene regulador de transmembrana de fibrose cística (cftr)
KR20200071198A (ko) 2018-12-10 2020-06-19 네오이뮨텍, 인코퍼레이티드 Nrf2 발현 조절 기반 T 세포 항암면역치료법
GB201820109D0 (en) 2018-12-11 2019-01-23 Vib Vzw Plants with a lignin trait and udp-glycosyltransferase mutation
US12274205B2 (en) 2018-12-12 2025-04-15 Monsanto Technology Llc Delayed harvest of short stature corn plants
US11384344B2 (en) 2018-12-17 2022-07-12 The Broad Institute, Inc. CRISPR-associated transposase systems and methods of use thereof
CN113423831B (zh) 2018-12-20 2023-03-10 瑞泽恩制药公司 核酸酶介导的重复扩增
WO2020132659A1 (en) 2018-12-21 2020-06-25 Precision Biosciences, Inc. Genetic modification of the hydroxyacid oxidase 1 gene for treatment of primary hyperoxaluria
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
AU2020205717B2 (en) 2019-01-11 2025-09-11 Acuitas Therapeutics, Inc. Lipids for lipid nanoparticle delivery of active agents
CA3145881A1 (en) 2019-01-15 2020-07-23 Seminis Vegetable Seeds, Inc. Green bean plants with improved resistance to sclerotinia sclerotiorum and genetic markers therefor
US12156877B1 (en) 2019-01-15 2024-12-03 The Regents Of The University Of California Methods of treating conditions related to a thiamine deficiency, a thiamine-dependent enzyme, or an associated cofactor
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
GB201900940D0 (en) 2019-01-23 2019-03-13 British American Tobacco Investments Ltd Method
EP3918080A1 (en) 2019-01-29 2021-12-08 The University Of Warwick Methods for enhancing genome engineering efficiency
JP7611151B2 (ja) 2019-02-06 2025-01-09 ヴィルモラン・エ・シエ 細胞質雄性不稔を司る新たな遺伝子
WO2020172343A2 (en) 2019-02-19 2020-08-27 Massachusetts Institute Of Technology Methods for treating injuries
WO2020178193A1 (en) 2019-03-01 2020-09-10 INSERM (Institut National de la Santé et de la Recherche Médicale) Method of treatment of sarcoidosis
EP3935179A4 (en) 2019-03-07 2022-11-23 The Trustees of Columbia University in the City of New York Rna-guided dna integration using tn7-like transposons
EP3708651A1 (en) 2019-03-12 2020-09-16 KWS SAAT SE & Co. KGaA Improving plant regeneration
US12419915B2 (en) 2019-03-13 2025-09-23 The Broad Institute, Inc. Microglial progenitors for regeneration of functional microglia in the central nervous system and therapeutics uses thereof
EP3942042A1 (en) 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US11001831B2 (en) 2019-03-25 2021-05-11 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
WO2020198174A1 (en) 2019-03-25 2020-10-01 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
PH12021500032A1 (en) 2019-04-03 2022-05-02 Regeneron Pharma Methods and compositions for insertion of antibody coding sequences into a safe harbor locus
IL299249B2 (en) 2019-04-03 2024-01-01 Prec Biosciences Inc Genetically-modified immune cells comprising a microrna-adapted shrna (shrnamir)
IL286917B (en) 2019-04-04 2022-09-01 Regeneron Pharma Methods for scarless introduction of targeted modifications into targeting vectors
CN117178959A (zh) 2019-04-04 2023-12-08 瑞泽恩制药公司 包括人源化凝血因子12基因座的非人动物
US20220204994A1 (en) 2019-04-05 2022-06-30 Precision Biosciences, Inc. Methods of preparing populations of genetically-modified immune cells
EP3950942A4 (en) 2019-04-05 2023-01-18 Osaka University PROCEDURE FOR MAKING A KNOCK-IN CELL
EP3956349A1 (en) 2019-04-17 2022-02-23 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
BR112021022411A2 (pt) 2019-05-13 2022-03-15 Kws Saat Se & Co Kgaa Tolerância à seca em milho
GB201906768D0 (en) 2019-05-14 2019-06-26 British American Tobacco Investments Ltd Method
PH12021553009A1 (en) 2019-05-23 2022-11-07 Univ Nanjing Agricultural Methods for improving rice grain yield
AR118995A1 (es) 2019-05-25 2021-11-17 Kws Saat Se & Co Kgaa Mejorador de la inducción de haploides
WO2020243371A1 (en) 2019-05-28 2020-12-03 Massachusetts Institute Of Technology Methods and compositions for modulating immune responses
AU2020284555A1 (en) 2019-05-28 2021-12-23 Selecta Biosciences, Inc. Methods and compositions for attenuated anti-viral transfer vector immune response
WO2020243661A1 (en) 2019-05-31 2020-12-03 The Broad Institute, Inc. Methods for treating metabolic disorders by targeting adcy5
AU2020286382A1 (en) 2019-06-04 2021-11-04 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized TTR locus with a beta-slip mutation and methods of use
WO2020244759A1 (en) 2019-06-05 2020-12-10 Klemm & Sohn Gmbh & Co. Kg New plants having a white foliage phenotype
AU2020288623A1 (en) 2019-06-06 2022-01-06 Inscripta, Inc. Curing for recursive nucleic acid-guided cell editing
MX2021015122A (es) 2019-06-07 2022-04-06 Regeneron Pharma Animales no humanos que comprenden un locus de albumina humanizado.
JP2022536364A (ja) 2019-06-13 2022-08-15 ザ ジェネラル ホスピタル コーポレイション 操作されたヒト内在性ウイルス様粒子および細胞への送達のためのその使用方法
ES3034102T3 (en) 2019-06-14 2025-08-13 Regeneron Pharma Models of tauopathy
EP3987024A4 (en) 2019-06-20 2023-11-01 University Of Massachusetts COMPOSITIONS AND METHODS FOR ENHANCED GENOMIC EDITING
AU2020297499A1 (en) 2019-06-21 2022-02-03 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli
WO2020254850A1 (en) 2019-06-21 2020-12-24 Vilmorin & Cie Improvement of quality and permanence of green color of peppers at maturity and over-maturity
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
MX2022000016A (es) 2019-06-27 2022-02-24 Crispr Therapeutics Ag Uso de linfocitos t con receptor de antigeno quimerico e inhibidores de linfocitos citoliticos naturales para el tratamiento del cancer.
EP3757219A1 (en) 2019-06-28 2020-12-30 KWS SAAT SE & Co. KGaA Enhanced plant regeneration and transformation by using grf1 booster gene
GB201909562D0 (en) 2019-07-03 2019-08-14 British American Tobacco Investments Ltd Method
CN114450265B (zh) 2019-07-03 2024-12-24 菲克特生物科学股份有限公司 阳离子脂质及其用途
GB201909563D0 (en) 2019-07-03 2019-08-14 British American Tobacco Investments Ltd Method
WO2021004938A1 (en) 2019-07-05 2021-01-14 Biogemma Method for increasing yield in plants
AU2020310877B2 (en) 2019-07-11 2024-05-23 Consejo Nacional De Investigaciones Científicas Y Técnicas Methods for improved regeneration of transgenic plants using Growth-Regulating Factor (GRF), GRF-Interacting Factor (GIF), or chimeric GRF-GIF genes and proteins
WO2021013950A1 (en) 2019-07-23 2021-01-28 Mnemo Therapeutics Immune cells defective for suv39h1
FR3099178A1 (fr) 2019-07-24 2021-01-29 Soltis Tournesol à teneur élevée en acide oléique et procédé d’obtention
EP4004216A1 (en) 2019-07-25 2022-06-01 Precision BioSciences, Inc. Compositions and methods for sequential stacking of nucleic acid sequences into a genomic locus
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
US12344850B2 (en) 2019-07-30 2025-07-01 Pairwise Plants Services, Inc. Morphogenic regulators and methods of using the same
WO2021019272A1 (en) 2019-07-31 2021-02-04 Vilmorin & Cie Tolerance to tolcndv in cucumber
EP3772542A1 (en) 2019-08-07 2021-02-10 KWS SAAT SE & Co. KGaA Modifying genetic variation in crops by modulating the pachytene checkpoint protein 2
JP2022544825A (ja) 2019-08-20 2022-10-21 プレシジョン バイオサイエンシズ，インク． 細胞免疫療法のためのリンパ球枯渇投与レジメン
WO2021035170A1 (en) 2019-08-21 2021-02-25 Precision Biosciences, Inc. Compositions and methods for tcr reprogramming using fusion proteins
US20220298501A1 (en) 2019-08-30 2022-09-22 The Broad Institute, Inc. Crispr-associated mu transposase systems
KR20220101073A (ko) 2019-08-30 2022-07-19 예일 유니버시티 핵산을 세포로 전달하기 위한 조성물 및 방법
BR112022003891A2 (pt) 2019-09-06 2022-05-31 Crispr Therapeutics Ag Células t geneticamente manipuladas possuindo persistência melhorada em cultura
AU2020355000A1 (en) 2019-09-23 2022-03-17 Omega Therapeutics, Inc. Compositions and methods for modulating apolipoprotein B (APOB) gene expression
JP2022548399A (ja) 2019-09-23 2022-11-18 オメガ セラピューティクス， インコーポレイテッド 肝細胞核因子４－アルファ（ＨＮＦ４α）遺伝子発現をモジュレートするための組成物および方法
SG10202008262UA (en) 2019-09-26 2021-04-29 Seminis Vegetable Seeds Inc Lettuce plants having resistance to nasonovia ribisnigri biotype nr:1
GB201914137D0 (en) 2019-10-01 2019-11-13 Univ Leeds Innovations Ltd Modified Plants
US11981922B2 (en) 2019-10-03 2024-05-14 Dana-Farber Cancer Institute, Inc. Methods and compositions for the modulation of cell interactions and signaling in the tumor microenvironment
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021074367A1 (en) 2019-10-17 2021-04-22 KWS SAAT SE & Co. KGaA Enhanced disease resistance of crops by downregulation of repressor genes
EP3808170A1 (en) 2019-10-17 2021-04-21 Bejo Zaden B.V. Lactuca sativa resistance to bremia lactucae
US20220411479A1 (en) 2019-10-30 2022-12-29 Precision Biosciences, Inc. Cd20 chimeric antigen receptors and methods of use for immunotherapy
CN112779266A (zh) 2019-11-06 2021-05-11 青岛清原化合物有限公司 在生物体内创制新基因的方法及应用
IL292605B2 (en) 2019-11-08 2025-09-01 Regeneron Pharma CRISPR and AAV strategies for treating childhood X-linked retinoschisis
JP7753203B2 (ja) 2019-11-12 2025-10-14 カー・ヴェー・エス ザート エス・エー ウント コー． カー・ゲー・アー・アー ヘテロデラ属の病原体に対する耐性遺伝子
EP4057802A1 (en) 2019-11-14 2022-09-21 Vilmorin & Cie Resistance to acidovorax valerianellae in corn salad
WO2021095847A1 (ja) 2019-11-14 2021-05-20 国立大学法人広島大学 抗原特異的受容体遺伝子を環状ｄｎａを用いてｔ細胞ゲノムに導入する方法
JP7448120B2 (ja) 2019-11-14 2024-03-12 国立研究開発法人農業・食品産業技術総合研究機構 プラズマを用いてゲノム編集酵素を植物細胞内に導入する方法
WO2021102059A1 (en) 2019-11-19 2021-05-27 Inscripta, Inc. Methods for increasing observed editing in bacteria
WO2021108363A1 (en) 2019-11-25 2021-06-03 Regeneron Pharmaceuticals, Inc. Crispr/cas-mediated upregulation of humanized ttr allele
AU2020391435A1 (en) 2019-11-27 2022-06-23 Cibus Europe B.V. Tal-effector nucleases for gene editing
US20230036065A1 (en) 2019-12-06 2023-02-02 Precision Biosciences, Inc. Methods for cancer immunotherapy
AU2020402526B2 (en) 2019-12-10 2025-01-23 Inscripta, Inc. Novel MAD nucleases
US10704033B1 (en) 2019-12-13 2020-07-07 Inscripta, Inc. Nucleic acid-guided nucleases
EP4074821A4 (en) 2019-12-13 2024-01-17 Chugai Seiyaku Kabushiki Kaisha SYSTEM FOR DETECTION OF EXTRACELLULAR PURINERGICAL RECEPTOR LIGAND AND NON-HUMAN ANIMAL TO WHICH THE LATTER WAS TRANSFERRED
EP3835309A1 (en) 2019-12-13 2021-06-16 KWS SAAT SE & Co. KGaA Method for increasing cold or frost tolerance in a plant
CN113061626B (zh) * 2019-12-16 2024-01-19 中国科学院脑科学与智能技术卓越创新中心 一种组织特异性敲除斑马鱼基因的方法及应用
IL292895A (en) 2019-12-18 2022-07-01 Inscripta Inc Cascade/dcas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells
GB201918902D0 (en) 2019-12-19 2020-02-05 Genome Res Ltd Cell differentiation
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
US12165747B2 (en) 2020-01-23 2024-12-10 The Broad Institute, Inc. Molecular spatial mapping of metastatic tumor microenvironment
AU2021213705A1 (en) 2020-01-27 2022-06-16 Inscripta, Inc. Electroporation modules and instrumentation
BR112022014799A2 (pt) 2020-01-31 2022-09-20 Calyxt Inc Planta de soja, parte de planta ou célula de planta, método para gerar uma planta de soja e composição de óleo de soja
EP4100525A1 (en) 2020-02-05 2022-12-14 Institut National de la Santé et de la Recherche Médicale (INSERM) Methods of treatment of cancer disease by targeting an epigenetic factor
WO2021158915A1 (en) 2020-02-06 2021-08-12 Precision Biosciences, Inc. Recombinant adeno-associated virus compositions and methods for producing and using the same
US20230091338A1 (en) 2020-02-24 2023-03-23 Pioneer Hi-Bred International, Inc. Intra-genomic homologous recombination
BR112022017197A2 (pt) 2020-02-28 2022-11-01 Pioneer Hi Bred Int Sistema de produção haploide duplicado de sorgo
CA3169128A1 (en) 2020-02-28 2021-09-02 Ling MENG Immature inflorescence meristem editing
CN115485385A (zh) 2020-03-04 2022-12-16 瑞泽恩制药公司 用于使肿瘤细胞对免疫疗法敏感的方法和组合物
WO2021183636A1 (en) 2020-03-10 2021-09-16 Calyxt, Inc. Transformation and regeneration of cannabaceae
WO2021183720A1 (en) 2020-03-11 2021-09-16 Omega Therapeutics, Inc. Compositions and methods for modulating forkhead box p3 (foxp3) gene expression
EP4129050A4 (en) 2020-03-26 2024-05-08 National Agriculture And Food Research Organization METHOD FOR PRODUCING A TEMPERATURE SENSITIVE MALE STERILE PLANT
CA3172449A1 (en) 2020-03-27 2021-09-30 Erik Hans MANTING Ex vivo use of modified cells of leukemic origin for enhancing the efficacy of adoptive cell therapy
CN115697044A (zh) 2020-03-31 2023-02-03 艾洛生物系统有限公司 西瓜和其他葫芦科中内源罗汉果苷途径基因的调控
WO2021202648A1 (en) 2020-03-31 2021-10-07 Calyxt, Inc. Agrobacterium-mediated infiltration of cannabaceae
JP2023521055A (ja) 2020-04-09 2023-05-23 アール・ジエイ・レイノルズ・タバコ・カンパニー ニコチアナ・タバカムにおけるニコチンレベルの調整方法
EP4135511A1 (en) 2020-04-14 2023-02-22 Academisch Ziekenhuis Leiden (h.o.d.n. LUMC) Methods for induction of endogenous tandem duplication events
NL2025344B1 (en) 2020-04-14 2021-10-26 Academisch Ziekenhuis Leiden Methods for induction of endogenous tandem duplication events
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
WO2021224416A1 (en) 2020-05-06 2021-11-11 Cellectis S.A. Methods to genetically modify cells for delivery of therapeutic proteins
EP4146797A1 (en) 2020-05-06 2023-03-15 Orchard Therapeutics (Europe) Limited Treatment for neurodegenerative diseases
EP4146812A1 (en) 2020-05-06 2023-03-15 Cellectis S.A. Methods for targeted insertion of exogenous sequences in cellular genomes
WO2021226558A1 (en) 2020-05-08 2021-11-11 The Broad Institute, Inc. Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
US20230183664A1 (en) 2020-05-11 2023-06-15 Precision Biosciences, Inc. Self-limiting viral vectors encoding nucleases
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
BR112022023443A2 (pt) 2020-05-29 2022-12-20 Kws Saat Se & Co Kgaa Indução de haploides em plantas
WO2021245435A1 (en) 2020-06-03 2021-12-09 Vilmorin & Cie Melon plants resistant to scab disease, aphids and powdery mildew
JP2023530234A (ja) 2020-06-05 2023-07-14 ザ・ブロード・インスティテュート・インコーポレイテッド 新生物を治療するための組成物および方法
IL298618A (en) 2020-06-05 2023-01-01 Vilmorin & Cie Tobrfv resistance of Solanum lycopersicum plants
AU2021292695A1 (en) 2020-06-19 2023-02-02 Fate Therapeutics, Inc. Combining iPSC-derived effector cell types for immunotherapy use
WO2021260139A1 (en) 2020-06-25 2021-12-30 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods of treatment and diagnostic of pathological conditions associated with intense stress
CN116133679A (zh) 2020-06-30 2023-05-16 门德斯有限公司 白血病衍生细胞在卵巢癌疫苗中的用途
EP4181664A1 (en) 2020-07-14 2023-05-24 KWS SAAT SE & Co. KGaA Methods for identifying and selecting maize plants with resistance to northern corn leaf blight
AU2021204717A1 (en) 2020-07-15 2022-02-03 Seminis Vegetable Seeds, Inc. Green Bean Plants with Improved Disease Resistance
CA3189338A1 (en) 2020-07-16 2022-01-20 Acuitas Therapeutics, Inc. Cationic lipids for use in lipid nanoparticles
WO2022020800A2 (en) 2020-07-24 2022-01-27 The General Hospital Corporation Enhanced virus-like particles and methods of use thereof for delivery to cells
EP4188395A1 (en) 2020-07-30 2023-06-07 Institut Curie Immune cells defective for socs1
EP4192875A1 (en) 2020-08-10 2023-06-14 Precision BioSciences, Inc. Antibodies and fragments specific for b-cell maturation antigen and uses thereof
EP4501951A3 (en) 2020-08-25 2025-04-30 Kite Pharma, Inc. T cells with improved functionality
JP2023544970A (ja) 2020-08-31 2023-10-26 イエール ユニバーシティ 細胞への核酸の送達のための組成物及び方法
CA3194019A1 (en) 2020-09-04 2022-03-10 National University Corporation Kobe University Miniaturized cytidine deaminase-containing complex for modifying double-stranded dna
US20230340149A1 (en) 2020-09-07 2023-10-26 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods of treatment of inflammatory bowel diseases
EP4214314A4 (en) 2020-09-15 2024-10-16 Inscripta, Inc. Crispr editing to embed nucleic acid landing pads into genomes of live cells
EP3971295A1 (en) 2020-09-16 2022-03-23 FRAUNHOFER-GESELLSCHAFT zur Förderung der angewandten Forschung e.V. Methods for the production of genome edited plants
KR20230074515A (ko) 2020-09-23 2023-05-30 크리스퍼 테라퓨틱스 아게 개선된 기능성 및 지속성을 갖는 붕괴된 레그나제-1 및/또는 tgfbrii를 지니는 유전자 조작된 t 세포
JP2023543803A (ja) 2020-09-24 2023-10-18 ザ ブロード インスティテュート，インコーポレーテッド プライム編集ガイドｒｎａ、その組成物、及びその使用方法
IL301846A (en) 2020-10-02 2023-06-01 Vilmorin & Cie A hotel with an extended shelf life
US20230365995A1 (en) 2020-10-07 2023-11-16 Precision Biosciences, Inc. Lipid nanoparticle compositions
CR20230175A (es) 2020-10-23 2023-07-26 Elo Life Systems Inc Métodos para producir plantas de vainilla con mejor sabor y producción agronómica
CN116406383A (zh) 2020-11-06 2023-07-07 日商基因编辑力股份有限公司 FokI核酸酶结构域的突变体
EP4240874A1 (en) 2020-11-06 2023-09-13 Institut National de la Santé et de la Recherche Médicale (INSERM) Methods for diagnosis and treating polycystic ovary syndrome (pcos)
US11512297B2 (en) 2020-11-09 2022-11-29 Inscripta, Inc. Affinity tag for recombination protein recruitment
WO2022103799A1 (en) 2020-11-10 2022-05-19 Industrial Microbes, Inc. Microorganisms capable of producing poly(hiba) from feedstock
WO2022103785A1 (en) 2020-11-10 2022-05-19 Mayo Foundation For Medical Education And Research Methods and materials for treating heart failure
WO2022109316A1 (en) 2020-11-20 2022-05-27 Revivicor, Inc. Multi-transgenic pigs with growth hormone receptor knockout for xenotransplantation
EP4251282A1 (en) 2020-11-27 2023-10-04 Institut National de la Santé et de la Recherche Médicale (INSERM) Methods for diagnosis and monitoring of toxic epidermal necrolysis
US20230416786A1 (en) 2020-11-30 2023-12-28 Cellectis Sa Use of aminoquinoline compounds for higher gene integration
US11661459B2 (en) 2020-12-03 2023-05-30 Century Therapeutics, Inc. Artificial cell death polypeptide for chimeric antigen receptor and uses thereof
CA3201621A1 (en) 2020-12-03 2022-06-09 Century Therapeutics, Inc. Genetically engineered cells and uses thereof
EP4255172A1 (en) 2020-12-03 2023-10-11 Vilmorin & Cie Tomato plants resistant to tobrfv, tmv, tomv and tommv and corresponding resistance genes
EP4019639A1 (en) 2020-12-22 2022-06-29 KWS SAAT SE & Co. KGaA Promoting regeneration and transformation in beta vulgaris
EP4019638A1 (en) 2020-12-22 2022-06-29 KWS SAAT SE & Co. KGaA Promoting regeneration and transformation in beta vulgaris
US20220193134A1 (en) 2020-12-23 2022-06-23 Crispr Therapeutics Ag Co-use of lenalidomide with car-t cells
US20240316090A1 (en) 2020-12-28 2024-09-26 Arcturus Therapeutics, Inc. Transcription activator-like effector nucleases (talens) targeting hbv
CA3204158A1 (en) 2021-01-04 2022-07-07 Juhan Kim Mad nucleases
US20240376451A1 (en) 2021-01-07 2024-11-14 Inscripta, Inc. Mad nucleases
WO2022150790A2 (en) 2021-01-11 2022-07-14 The Broad Institute, Inc. Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision
US20240318159A1 (en) 2021-01-12 2024-09-26 Mitolab Inc. Context-dependent, double-stranded dna-specific deaminases and uses thereof
CN116981697A (zh) 2021-01-14 2023-10-31 森迪生物科学公司 可分泌有效载荷调节
CA3203705A1 (en) 2021-01-22 2022-07-28 Erik Hans MANTING Methods of tumor vaccination
US20250127811A1 (en) 2021-01-28 2025-04-24 Precision Biosciences, Inc. Modulation of tgf beta signaling in genetically-modified eukaryotic cells
US11884924B2 (en) 2021-02-16 2024-01-30 Inscripta, Inc. Dual strand nucleic acid-guided nickase editing
EP4305153A1 (en) 2021-03-09 2024-01-17 CRISPR Therapeutics AG Genetically engineered t cells with ptpn2 knockout have improved functionality and anti-tumor activity
WO2022190058A1 (en) 2021-03-12 2022-09-15 Dcprime B.V. Methods of vaccination and use of cd47 blockade
WO2022198093A1 (en) 2021-03-18 2022-09-22 Calyxt, Inc. Producing albumin using plant cell matrices
WO2022198107A1 (en) 2021-03-18 2022-09-22 Calyxt, Inc. Producing betalain in cannabaceae plant parts
WO2022198094A1 (en) 2021-03-18 2022-09-22 Calyxt, Inc. Producing albumin in cannabaceae plant parts
WO2022198106A1 (en) 2021-03-18 2022-09-22 Calyxt, Inc. Producing betalains using plant cell matrices
US20240191245A1 (en) 2021-03-18 2024-06-13 Calyxt, Inc. Plant cell matrices and methods thereof
WO2022208489A1 (en) 2021-04-02 2022-10-06 Vilmorin & Cie Semi-determinate or determinate growth habit trait in cucurbita
US20220333074A1 (en) 2021-04-07 2022-10-20 Century Therapeutics, Inc. Compositions and Methods for Generating Alpha-Beta T Cells from Induced Pluripotent Stem Cells
CA3214661A1 (en) 2021-04-07 2022-10-13 Century Therapeutics, Inc. Combined artificial cell death/reporter system polypeptide for chimeric antigen receptor cell and uses thereof
WO2022216514A1 (en) 2021-04-07 2022-10-13 Century Therapeutics, Inc. Compositions and methods for generating gamma-delta t cells from induced pluripotent stem cells
WO2022218998A1 (en) 2021-04-13 2022-10-20 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for treating hepatitis b and d virus infection
US20240141311A1 (en) 2021-04-22 2024-05-02 North Carolina State University Compositions and methods for generating male sterile plants
AU2022261125A1 (en) 2021-04-23 2023-11-23 University Of Rochester Genome editing by directed non-homologous dna insertion using a retroviral integrase-cas fusion protein and methods of treatment
US20240197880A1 (en) 2021-04-30 2024-06-20 Cellectis S.A. New anti-muc1 cars and gene edited immune cells for solid tumors cancer immunotherapy
EP4337769A1 (en) 2021-05-10 2024-03-20 SQZ Biotechnologies Company Methods for delivering genome editing molecules to the nucleus or cytosol of a cell and uses thereof
WO2022251644A1 (en) 2021-05-28 2022-12-01 Lyell Immunopharma, Inc. Nr4a3-deficient immune cells and uses thereof
US20230052243A1 (en) 2021-06-02 2023-02-16 Lyell Immunopharma, Inc. Nr4a-deficient cells expressing c-jun and uses thereof
WO2022271548A2 (en) 2021-06-23 2022-12-29 Massachusetts Institute Of Technology Compositions, methods and systems for the delivery of gene editing material to cells
JP2024523759A (ja) 2021-07-07 2024-06-28 サイバス オイローペ ベスローテン ヴェンノーツハップ インテインと融合した転写活性化因子様エフェクター
WO2023283359A2 (en) 2021-07-07 2023-01-12 Omega Therapeutics, Inc. Compositions and methods for modulating secreted frizzled receptor protein 1 (sfrp1) gene expression
CA3221517A1 (en) 2021-07-30 2023-02-02 Monika KLOIBER-MAITZ Plants with improved digestibility and marker haplotypes
MX2024001624A (es) 2021-08-06 2024-04-30 Vilmorin & Cie Resistencia a leveillula taurica en el pimiento.
JP7125727B1 (ja) 2021-09-07 2022-08-25 国立大学法人千葉大学 核酸配列改変用組成物および核酸配列の標的部位を改変する方法
US20230128917A1 (en) 2021-09-14 2023-04-27 Crispr Therapeutics Ag Genetically engineered immune cells having a disrupted cd83 gene
CN118284700A (zh) 2021-09-17 2024-07-02 希博斯欧洲有限公司 转化大麻科细胞
EP4404740A1 (en) 2021-09-20 2024-07-31 Revivicor Inc. Multitransgenic pigs comprising ten genetic modifications for xenotransplantation
CN118284701A (zh) 2021-09-27 2024-07-02 孟山都技术公司 用于转化从单子叶植物种子切除的胚外植体的组合物和方法
GB202113933D0 (en) 2021-09-29 2021-11-10 Genome Res Ltd Methods for gene editing
US20230141563A1 (en) 2021-10-12 2023-05-11 Selecta Biosciences, Inc. Methods and compositions for attenuating anti-viral transfer vector igm responses
IL311962A (en) 2021-10-14 2024-06-01 Lonza Sales Ag Modified producer cells for extracellular vesicle production
EP4416292A2 (en) 2021-10-14 2024-08-21 Arsenal Biosciences, Inc. Immune cells having co-expressed shrnas and logic gate systems
WO2023064872A1 (en) 2021-10-14 2023-04-20 Precision Biosciences, Inc. Combinations of anti-bcma car t cells and gamma secretase inhibitors
WO2023070003A1 (en) 2021-10-19 2023-04-27 Precision Biosciences, Inc. Gene editing methods for treating alpha-1 antitrypsin (aat) deficiency
US20230133695A1 (en) 2021-10-20 2023-05-04 University Of Rochester Methods and compositions for rejuvenating cns glial populations by suppresion of transcription factors
CA3234404A1 (en) 2021-10-20 2023-04-27 Steven A. Goldman Treatment with genetically modified cells, and genetically modified cells per se, with increased competitive advantage and/or decreased competitive disadvantage
WO2023070043A1 (en) 2021-10-20 2023-04-27 Yale University Compositions and methods for targeted editing and evolution of repetitive genetic elements
JP2024540987A (ja) 2021-10-21 2024-11-06 バーテックス ファーマシューティカルズ インコーポレイテッド 低免疫細胞
JP2024540411A (ja) 2021-11-08 2024-10-31 プロジェントス・セラピューティクス・インコーポレイテッド 血小板由来成長因子受容体（ｐｄｇｆｒ）アルファ阻害剤及びその使用
EP4430206A1 (en) 2021-11-10 2024-09-18 Encodia, Inc. Methods for barcoding macromolecules in individual cells
WO2023091910A1 (en) 2021-11-16 2023-05-25 Precision Biosciences, Inc. Methods for cancer immunotherapy
CA3238700A1 (en) 2021-11-23 2022-11-23 Philippe Duchateau New tale protein scaffolds with improved on-target/off-target activity ratios
WO2023093862A1 (en) 2021-11-26 2023-06-01 Epigenic Therapeutics Inc. Method of modulating pcsk9 and uses thereof
US20250027097A1 (en) 2021-11-30 2025-01-23 Pioneer Hi-Bred International, Inc. High efficiency large scale chromosomal genome manipulation
GB202117314D0 (en) 2021-11-30 2022-01-12 Clarke David John Cyclic nucleic acid fragmentation
JP2024544013A (ja) 2021-12-03 2024-11-26 ザ ブロード インスティテュート，インコーポレーテッド 有効なｉｎ ｖｉｖｏ送達のための組成物および方法
US20250382334A1 (en) 2021-12-03 2025-12-18 The Broad Institute, Inc. Self-assembling virus-like particles for delivery of nucleic acid programmable fusion proteins and methods of making and using same
US20230193310A1 (en) 2021-12-10 2023-06-22 Seminis Vegetabe Seeds, Inc. Lettuce plants having resistance to downy mildew
GB202118058D0 (en) 2021-12-14 2022-01-26 Univ Warwick Methods to increase yields in crops
EP4450634A4 (en) 2021-12-15 2025-12-24 Nanjing Genscript Biotech Co Ltd INTEGRATION SITE DETECTION METHOD
WO2023111913A1 (en) 2021-12-15 2023-06-22 Crispr Therapeutics Ag Engineered anti-liv1 cell with regnase-1 and/or tgfbrii disruption
WO2023114943A2 (en) 2021-12-16 2023-06-22 Acuitas Therapeutics, Inc. Lipids for use in lipid nanoparticle formulations
US20250087303A1 (en) * 2021-12-17 2025-03-13 Altius Institute For Biomedical Sciences Nucleic Acid Sequences Encoding Repeated Sequences Resistant to Recombination in Viruses
WO2023119201A2 (en) 2021-12-22 2023-06-29 Crispr Therapeutics Ag Genetically engineered t cells with disrupted casitas b-lineage lymphoma proto-oncogene-b (cblb) and uses thereof
EP4456910A1 (en) 2021-12-28 2024-11-06 Mnemo Therapeutics Immune cells with inactivated suv39h1 and modified tcr
CA3243966A1 (en) 2021-12-29 2023-07-06 Century Therapeutics, Inc. Genetically modified cells with anti-CD19/anti-CD22 chimeric antigenic receptors, and their uses
EP4457342A1 (en) 2021-12-29 2024-11-06 Bristol-Myers Squibb Company Generation of landing pad cell lines
EP4472648A1 (en) 2022-02-01 2024-12-11 University of Rochester Methods of generating a population of neurons from human glial progenitor cells and genetic constructs for carrying out such methods
JP2025505600A (ja) 2022-02-03 2025-02-28 ニコベンチャーズ トレーディング リミテッド タバコ植物のアルカロイド含有量を調節する方法
WO2023148476A1 (en) 2022-02-03 2023-08-10 Nicoventures Trading Limited Method of modulating alkaloid content in tobacco plants
JP2025505606A (ja) 2022-02-04 2025-02-28 ニコベンチャーズ トレーディング リミテッド タバコ植物のアルカロイド含有量を調節する方法
US20250161413A1 (en) 2022-02-16 2025-05-22 Dana-Farber Cancer Institute, Inc. Methods for decreasing pathologic alpha-synuclein using agents that modulate fndc5 or biologically active fragments thereof
EP4486890A1 (en) 2022-03-01 2025-01-08 CRISPR Therapeutics AG Methods and compositions for treating angiopoietin-like 3 (angptl3) related conditions
US20230357437A1 (en) 2022-03-09 2023-11-09 Selecta Biosciences, Inc. Immunosuppressants in combination with anti-igm agents and related dosing
AU2023240501A1 (en) 2022-03-21 2024-10-31 Crispr Therapeutics Ag Methods and compositions for treating lipoprotein-related diseases
US20230303713A1 (en) 2022-03-23 2023-09-28 Crispr Therapeutics Ag Anti-cd19 car-t cells with multiple gene edits and therapeutic uses thereof
TW202346575A (zh) 2022-03-23 2023-12-01 瑞士商Ｃｒｉｓｐｒ治療公司 具有ｒｅｇｎａｓｅ－１及／或ｔｇｆｂｒｉｉ破壞的抗ｃｄ８３ ｃａｒ－ｔ細胞
EP4256950A1 (en) 2022-04-06 2023-10-11 Vilmorin et Cie Tolerance to cgmmv in cucumber
GB202205148D0 (en) 2022-04-07 2022-05-25 Nicoventures Trading Ltd Method
GB202205149D0 (en) 2022-04-07 2022-05-25 Nicoventures Trading Ltd Method
US20250222029A1 (en) 2022-04-08 2025-07-10 Fate Therapeutics, Inc. Chimeric antigen receptor for tumor targeting
CN119256075A (zh) 2022-04-08 2025-01-03 菲特治疗公司 具有实体瘤靶向骨架的细胞及其用途
GB202205562D0 (en) 2022-04-14 2022-06-01 Nicoventures Trading Ltd Method
GB202205561D0 (en) 2022-04-14 2022-06-01 Nicoventures Trading Ltd Method
GB202206107D0 (en) 2022-04-27 2022-06-08 Nicoventures Trading Ltd Method
GB202206109D0 (en) 2022-04-27 2022-06-08 Nicoventures Trading Ltd Method
JP2025515030A (ja) 2022-05-02 2025-05-13 フォンダジオン テレソン イーティーエス 遺伝子編集用の相同性非依存性標的組込み
CA3256953A1 (en) 2022-05-09 2023-11-16 Regeneron Pharmaceuticals, Inc. VECTORS AND METHODS FOR IN VIVO ANTIBODY PRODUCTION
CN120303407A (zh) 2022-05-17 2025-07-11 恩维洛普治疗有限责任公司 用于有效体内递送的组合物和方法
EP4525892A1 (en) 2022-05-19 2025-03-26 Lyell Immunopharma, Inc. Polynucleotides targeting nr4a3 and uses thereof
EP4279085A1 (en) 2022-05-20 2023-11-22 Mnemo Therapeutics Compositions and methods for treating a refractory or relapsed cancer or a chronic infectious disease
EP4278891A1 (en) 2022-05-20 2023-11-22 KWS SAAT SE & Co. KGaA Clubroot resistance and markers in brassica
GB202207774D0 (en) 2022-05-26 2022-07-13 Cambridge Entpr Ltd Modified plants
WO2023240212A2 (en) 2022-06-08 2023-12-14 Century Therapeutics, Inc. Genetically engineered cells having anti-cd133 / anti-egfr chimeric antigen receptors, and uses thereof
CA3257650A1 (en) 2022-06-08 2023-12-14 Century Therapeutics, Inc. Immunoeffector cells derived from genetically modified, multipotential stem cells with membrane IL-12 and their uses
CA3257424A1 (en) 2022-06-08 2023-12-14 Century Therapeutics, Inc. Genetically modified cells expressing variants of CD16 and NKG2D and their uses
AU2023290692A1 (en) 2022-06-17 2025-01-23 Crispr Therapeutics Ag LIPID NANOPARTICLES (LNPs)-BASED OCULAR DELIVERY
WO2023248145A1 (en) 2022-06-21 2023-12-28 Crispr Therapeutics Ag Compositions and methods for treating human immunodeficiency virus
US20230404003A1 (en) 2022-06-21 2023-12-21 Seminis Vegetable Seeds, Inc. Novel qtls conferring resistance to cucumber mosaic virus
WO2023248147A1 (en) 2022-06-21 2023-12-28 Crispr Therapeutics Ag Methods and compositions for in vivo editing of stem cells
CN119451992A (zh) 2022-06-29 2025-02-14 克里斯珀医疗股份公司 靶向gpc-3的嵌合抗原受体和表达其的免疫细胞用于治疗用途
GB2621813A (en) 2022-06-30 2024-02-28 Univ Newcastle Preventing disease recurrence in Mitochondrial replacement therapy
WO2024020360A1 (en) 2022-07-18 2024-01-25 Pairwise Plants Services, Inc. Mustard green plants named 'pwrg-1', 'pwrg-2,' and 'pwsgc'
WO2024020597A1 (en) 2022-07-22 2024-01-25 The Johns Hopkins University Dendrimer-enabled targeted intracellular crispr/cas system delivery and gene editing
WO2024023801A2 (en) 2022-07-29 2024-02-01 Crispr Therapeutics Ag Genetically engineered immune cells having disrupted transporter associated with antigen processing-1 (tap-1) gene
WO2024023804A2 (en) 2022-07-29 2024-02-01 Crispr Therapeutics Ag Genetically engineered immune cells having disrupted transporter associated with antigen processing binding protein (tapbp) gene
CN120659627A (zh) 2022-07-29 2025-09-16 瑞泽恩制药公司 用于转铁蛋白受体（tfr）介导的脑和肌肉递送的组合物和方法
WO2024023802A2 (en) 2022-07-29 2024-02-01 Crispr Therapeutics Ag Genetically engineered immune cells having disrupted transporter associated with antigen processing-2 (tap-2) gene
LU502613B1 (en) 2022-08-01 2024-02-01 Plant Bioscience Ltd Methods of altering the starch granule profile in plants
CA3261296A1 (en) 2022-08-05 2024-02-08 Regeneron Pharmaceuticals, Inc. TDP-43 VARIANTS RESISTANT TO AGGREGATION
WO2024042199A1 (en) 2022-08-26 2024-02-29 KWS SAAT SE & Co. KGaA Use of paired genes in hybrid breeding
WO2024062388A2 (en) 2022-09-20 2024-03-28 Crispr Therapeutics Ag Genetically engineered immune cells expressing chimeric antigen receptor targeting cd20
WO2024062138A1 (en) 2022-09-23 2024-03-28 Mnemo Therapeutics Immune cells comprising a modified suv39h1 gene
WO2024064958A1 (en) 2022-09-23 2024-03-28 Lyell Immunopharma, Inc. Methods for culturing nr4a-deficient cells
WO2024064952A1 (en) 2022-09-23 2024-03-28 Lyell Immunopharma, Inc. Methods for culturing nr4a-deficient cells overexpressing c-jun
GB202214410D0 (en) 2022-09-30 2022-11-16 Ivy Farm Tech Limited genetically modified cells
WO2024077174A1 (en) 2022-10-05 2024-04-11 Lyell Immunopharma, Inc. Methods for culturing nr4a-deficient cells
WO2024079157A1 (en) 2022-10-11 2024-04-18 KWS SAAT SE & Co. KGaA Virus and insect resistance and markers in barley
JP2025536260A (ja) 2022-10-11 2025-11-05 イェール ユニバーシティー 細胞透過性抗体の組成物および使用方法
JP2025534904A (ja) 2022-10-21 2025-10-21 キージーン ナムローゼ フェンノートシャップ 修飾ｒｎａによる植物細胞へのｒｎａトランスフェクション
WO2024102838A1 (en) 2022-11-09 2024-05-16 Century Therapeutics, Inc. Engineered interleukin-7 receptors and uses thereof
CN120435498A (zh) 2022-11-10 2025-08-05 世纪治疗股份有限公司 具有抗-nectin4嵌合抗原受体的基因工程化细胞及其用途
JP2025538220A (ja) 2022-11-14 2025-11-26 リジェネロン・ファーマシューティカルズ・インコーポレイテッド アストロサイトへの線維芽細胞増殖因子受容体３媒介送達のための組成物および方法
EP4619515A1 (en) 2022-11-17 2025-09-24 The Broad Institute, Inc. Prime editor delivery by aav
EP4626477A1 (en) 2022-12-01 2025-10-08 Yale University Stimuli-responsive traceless engineering platform for intracellular payload delivery
WO2024124044A1 (en) 2022-12-07 2024-06-13 The Brigham And Women’S Hospital, Inc. Compositions and methods targeting sat1 for enhancing anti¬ tumor immunity during tumor progression
WO2024121354A1 (en) 2022-12-08 2024-06-13 Keygene N.V. Duplex sequencing with covalently closed dna ends
WO2024138087A2 (en) 2022-12-23 2024-06-27 The Broad Institute, Inc. Methods and compositions for modulating cellular factors to increase prime editing efficiencies
EP4642225A1 (en) 2022-12-29 2025-11-05 Vilmorin & Cie Tomato plants resistant to resistance-breaking tswv strains and corresponding resistance genes
GB202219829D0 (en) 2022-12-29 2023-02-15 Ivy Farm Tech Limited Genetically manipulated cells
EP4652272A1 (en) 2023-01-18 2025-11-26 The Broad Institute Inc. Prime editing-mediated readthrough of premature termination codons (pert)
WO2024155745A1 (en) 2023-01-18 2024-07-25 The Broad Institute, Inc. Base editing-mediated readthrough of premature termination codons (bert)
GB202300905D0 (en) 2023-01-20 2023-03-08 Nicoventures Trading Ltd Method
EP4658061A1 (en) 2023-02-01 2025-12-10 United Beet Seeds Beet yellows virus resistance
WO2024170505A1 (en) 2023-02-13 2024-08-22 Institut National de la Santé et de la Recherche Médicale Methods of treatment of iron overload associated diseases
TW202440938A (zh) 2023-02-15 2024-10-16 美商喬木生物技術公司 用於抑制微管解聚蛋白2(stmn2)轉錄物中異常剪接的基因編輯方法
GB202303077D0 (en) 2023-03-02 2023-04-19 Univ Oslo Brassica plants with improved seed retention
WO2024192108A1 (en) 2023-03-14 2024-09-19 Evolveimmune Therapeutics, Inc. Genetically modified car t cells and methods of making and using the same
US12419265B2 (en) 2023-03-27 2025-09-23 Redsea Science And Technology Inc. Tomato plant designated ‘X21-02’
WO2024206821A1 (en) 2023-03-31 2024-10-03 Briacell Therapeutics Corp. Methods for enhancing the immunogenicity of cellular vaccines
WO2024209000A1 (en) 2023-04-04 2024-10-10 Keygene N.V. Linkers for duplex sequencing
MX2024004405A (es) 2023-04-14 2024-11-08 Seminis Vegetable Seeds Inc Métodos y composiciones de resistencia a peronospora en la espinaca
WO2024216116A1 (en) 2023-04-14 2024-10-17 Precision Biosciences, Inc. Muscle-specific expression cassettes
WO2024216118A1 (en) 2023-04-14 2024-10-17 Precision Biosciences, Inc. Muscle-specific expression cassettes
IT202300007968A1 (it) 2023-04-21 2024-10-21 Fond Telethon Ets Metodi di editing genomico e costrutti
WO2024226499A1 (en) 2023-04-24 2024-10-31 The Broad Institute, Inc. Compositions and methods for modifying fertility
WO2024223402A1 (en) 2023-04-24 2024-10-31 Institut National de la Santé et de la Recherche Médicale Biallelic gene mutations for the diagnosis of neonatal systemic hypertension
WO2024223900A1 (en) 2023-04-28 2024-10-31 Vilmorin & Cie Genetic determinants conferring improved tobrfv resistance
WO2024236547A1 (en) 2023-05-18 2024-11-21 Inceptor Bio, Llc Modified phagocytic cells expressing chimeric antigen receptors comprising a herpes virus entry mediator (hvem) co-stimulatory domain and uses thereof
WO2024243438A2 (en) 2023-05-23 2024-11-28 Omega Therapeutics, Inc. Compositions and methods for reducing cxcl9, cxcl10, and cxcl11 gene expression
WO2025003305A1 (en) 2023-06-28 2025-01-02 Vilmorin & Cie Pepper resistance to meloidogyne enterolobii
WO2025003461A1 (en) 2023-06-30 2025-01-02 Institut National de la Santé et de la Recherche Médicale Methods of treatment of metabolic disorders
WO2025022290A1 (en) 2023-07-21 2025-01-30 Crispr Therapeutics Ag Modulating expression of alas1 (5'-aminolevulinate synthase 1) gene
WO2025021893A1 (en) 2023-07-24 2025-01-30 Vilmorin & Cie Resistance to cysdv in melons
WO2025046513A1 (en) 2023-08-29 2025-03-06 Inceptor Bio, Llc Methods of manufacturing myeloid-derived cells from hematopoietic stem cells and compositions and uses thereof
EP4520334A1 (en) 2023-09-07 2025-03-12 Mnemo Therapeutics Methods and compositions for improving immune response
WO2025052001A1 (en) 2023-09-07 2025-03-13 Mnemo Therapeutics Methods and compositions for improving immune response
WO2025059215A1 (en) 2023-09-12 2025-03-20 Aadigen, Llc Methods and compositions for treating or preventing cancer
WO2025064408A1 (en) 2023-09-18 2025-03-27 The Broad Institute, Inc. Compositions and methods for treating cardiovascular disease
WO2025064678A2 (en) 2023-09-20 2025-03-27 The Broad Institute, Inc. Prime editing-mediated readthrough of frameshift mutations (perf)
GB202314578D0 (en) 2023-09-22 2023-11-08 Univ Manchester Methods of producing homoplasmic modified plants or parts thereof
WO2025101938A2 (en) 2023-11-10 2025-05-15 Century Therapeutics, Inc. Genetically engineered cells having multi-transmembrane domain chimeric antigen receptors utilizing g protein-coupled receptor scaffolds, and uses thereof
WO2025106626A1 (en) 2023-11-15 2025-05-22 Century Therapeutics, Inc. Genetically engineered cells expressing c-x-c chemokine receptor type 4, and uses thereof
WO2025128981A1 (en) 2023-12-14 2025-06-19 Sohm, Inc. Compositions and methods for genome editing
WO2025163107A1 (en) 2024-02-01 2025-08-07 Institut Gustave Roussy Immune cells defective for znf217 and uses thereof
WO2025186284A1 (en) 2024-03-05 2025-09-12 Vilmorin & Cie Increased yield in cucumber
WO2025186726A1 (en) 2024-03-05 2025-09-12 Crispr Therapeutics Ag Modulating expression of agt (angiotensinogen) gene
WO2025207798A1 (en) 2024-03-26 2025-10-02 Century Therapeutics, Inc. Genetically engineered cells having anti-cd123 chimeric antigen receptors, and uses thereof
WO2025207795A1 (en) 2024-03-26 2025-10-02 Juno Therapeutics, Inc. Genetically engineered cells having anti-cd33 / anti-cd123 chimeric antigen receptors, and uses thereof
WO2025215354A1 (en) 2024-04-08 2025-10-16 Ivy Farm Technologies Limited Method of culturing an animal cell
WO2025217398A1 (en) 2024-04-10 2025-10-16 Lyell Immunopharma, Inc. Methods for culturing cells with improved culture medium
US20250332260A1 (en) 2024-04-10 2025-10-30 Garuda Therapeutics, Inc. Immune compatible cells for allogeneic cell therapies to cover global, ethnic, or disease-specific populations
WO2025224715A1 (en) 2024-04-26 2025-10-30 King Abdullah Univeristy Of Science And Technology Methods for improving precise genome modification and reducing unwanted mutations by crispr-cas editing
WO2025235388A1 (en) 2024-05-06 2025-11-13 Regeneron Pharmaceuticals, Inc. Transgene genomic identification by nuclease-mediated long read sequencing
WO2025247951A1 (en) 2024-05-28 2025-12-04 Institut Gustave Roussy Fyn kinase inhibitors, combinations and uses thereof
US20250382627A1 (en) 2024-06-13 2025-12-18 J.R. Simplot Company Engineering remontant flowering in rosaceae

Family Cites Families (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4179337A (en) * 1973-07-20 1979-12-18 Davis Frank F Non-immunogenic polypeptides
US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US4761373A (en) 1984-03-06 1988-08-02 Molecular Genetics, Inc. Herbicide resistance in plants
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
ATE57390T1 (de) 1986-03-11 1990-10-15 Plant Genetic Systems Nv Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen.
US4975374A (en) * 1986-03-18 1990-12-04 The General Hospital Corporation Expression of wild type and mutant glutamine synthetase in foreign hosts
US5276268A (en) * 1986-08-23 1994-01-04 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US5273894A (en) 1986-08-23 1993-12-28 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US5013659A (en) * 1987-07-27 1991-05-07 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5006333A (en) 1987-08-03 1991-04-09 Ddi Pharmaceuticals, Inc. Conjugates of superoxide dismutase coupled to high molecular weight polyalkylene glycols
US5162602A (en) 1988-11-10 1992-11-10 Regents Of The University Of Minnesota Corn plants tolerant to sethoxydim and haloxyfop herbicides
US5501967A (en) * 1989-07-26 1996-03-26 Mogen International, N.V./Rijksuniversiteit Te Leiden Process for the site-directed integration of DNA into the genome of plants
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5767366A (en) 1991-02-19 1998-06-16 Louisiana State University Board Of Supervisors, A Governing Body Of Louisiana State University Agricultural And Mechanical College Mutant acetolactate synthase gene from Ararbidopsis thaliana for conferring imidazolinone resistance to crop plants
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5792640A (en) * 1992-04-03 1998-08-11 The Johns Hopkins University General method to clone hybrid restriction endonucleases using lig gene
US5487994A (en) * 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5356802A (en) * 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5591616A (en) 1992-07-07 1997-01-07 Japan Tobacco, Inc. Method for transforming monocotyledons
JPH08510375A (ja) 1993-02-12 1996-11-05 ザ・ジョンズ−ホプキンス・ユニバーシティー フラボバクテリウム・オケアノコイテス（ｆｏｋｉ）制限エンドヌクレアーゼの機能ドメイン
US6808904B2 (en) 1994-06-16 2004-10-26 Syngenta Participations Ag Herbicide-tolerant protox genes produced by DNA shuffling
US7285416B2 (en) * 2000-01-24 2007-10-23 Gendaq Limited Regulated gene expression in plants
US6326166B1 (en) * 1995-12-29 2001-12-04 Massachusetts Institute Of Technology Chimeric DNA-binding proteins
US5824497A (en) 1995-02-10 1998-10-20 Mcmaster University High efficiency translation of mRNA molecules
US5853973A (en) * 1995-04-20 1998-12-29 American Cyanamid Company Structure based designed herbicide resistant products
US6084155A (en) * 1995-06-06 2000-07-04 Novartis Ag Herbicide-tolerant protoporphyrinogen oxidase ("protox") genes
JPH10117776A (ja) * 1996-10-22 1998-05-12 Japan Tobacco Inc インディカイネの形質転換方法
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
GB9819693D0 (en) * 1998-09-10 1998-11-04 Zeneca Ltd Glyphosate formulation
US7013219B2 (en) * 1999-01-12 2006-03-14 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6534261B1 (en) * 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7070934B2 (en) * 1999-01-12 2006-07-04 Sangamo Biosciences, Inc. Ligand-controlled regulation of endogenous gene expression
CA2361191A1 (en) * 1999-02-03 2000-08-10 The Children's Medical Center Corporation Gene repair involving the induction of double-stranded dna cleavage at a chromosomal target site
US6451732B1 (en) 1999-06-04 2002-09-17 Syngenta, Limited Herbicidal compositions of glyphosate trimesium
ATE353361T1 (de) * 2000-04-28 2007-02-15 Sangamo Biosciences Inc Gezielten modifikation der chromatinstruktur
US6368227B1 (en) 2000-11-17 2002-04-09 Steven Olson Method of swinging on a swing
CA2435394C (en) * 2001-01-22 2018-01-09 Sangamo Biosciences, Inc. Modified zinc finger binding proteins
US7262054B2 (en) * 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
WO2009095742A1 (en) 2008-01-31 2009-08-06 Cellectis New i-crei derived single-chain meganuclease and uses thereof
AU2003218382B2 (en) * 2002-03-21 2007-12-13 Sangamo Therapeutics, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
US7361635B2 (en) * 2002-08-29 2008-04-22 Sangamo Biosciences, Inc. Simultaneous modulation of multiple genes
WO2004067753A2 (en) * 2003-01-28 2004-08-12 Cellectis Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof.
US7888121B2 (en) * 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US7189691B2 (en) * 2004-04-01 2007-03-13 The Administrators Of The Tulane Educational Fund Methods and compositions for treating leukemia
US7833769B2 (en) 2004-05-03 2010-11-16 New England Biolabs, Inc. McrA variants and uses thereof
WO2006042145A2 (en) * 2004-10-07 2006-04-20 Cornell Research Foundation, Inc. THE RICE BACTERIAL BLIGHT DISEASE RESISTANCE GENE xa5
WO2007060495A1 (en) 2005-10-25 2007-05-31 Cellectis I-crei homing endonuclease variants having novel cleavage specificity and use thereof
WO2007102618A1 (en) 2006-03-08 2007-09-13 Kyoto University Nucleic acid cleaving agent
WO2008010009A1 (en) * 2006-07-18 2008-01-24 Cellectis Meganuclease variants cleaving a dna target sequence from a rag gene and uses thereof
US8563314B2 (en) * 2007-09-27 2013-10-22 Sangamo Biosciences, Inc. Methods and compositions for modulating PD1
CN101815722B (zh) 2007-09-28 2015-11-25 双刃基金会 Bs3抗性基因和使用方法
WO2010054348A2 (en) * 2008-11-10 2010-05-14 Two Blades Foundation Pathogen-inducible promoters and their use in enhancing the disease resistance of plants
EP2206726A1 (en) * 2009-01-08 2010-07-14 Universite Joseph Fourier Non-invasive tools for detecting vulnerable atherosclerotic plaques
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
US20110239315A1 (en) * 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
US8772008B2 (en) * 2009-05-18 2014-07-08 Sangamo Biosciences, Inc. Methods and compositions for increasing nuclease activity
US20120178647A1 (en) 2009-08-03 2012-07-12 The General Hospital Corporation Engineering of zinc finger arrays by context-dependent assembly
KR20120038020A (ko) * 2009-08-11 2012-04-20 상가모 바이오사이언스 인코포레이티드 표적화 변형에 대한 동형접합성 유기체
KR101813981B1 (ko) * 2009-10-22 2018-01-04 다우 아그로사이언시즈 엘엘씨 지방산 생합성에 포함된 식물 유전자를 표적하는 조작된 징크 핑거 단백질
US8956828B2 (en) * 2009-11-10 2015-02-17 Sangamo Biosciences, Inc. Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
EP2504429B1 (en) * 2009-11-27 2016-04-20 BASF Plant Science Company GmbH Chimeric endonucleases and uses thereof
CN102762726A (zh) * 2009-11-27 2012-10-31 巴斯夫植物科学有限公司 嵌合内切核酸酶及其用途
BR112012012588B1 (pt) * 2009-11-27 2019-03-26 Basf Plant Science Company Gmbh Endonuclease, método para recombinação homóloga de polinucleotídeos e método para mutação direcionada de polinucleotídeos
NO2510096T3 (OSRAM) * 2009-12-10 2015-03-21
US20110203012A1 (en) * 2010-01-21 2011-08-18 Dotson Stanton B Methods and compositions for use of directed recombination in plant breeding
CA2788560A1 (en) 2010-02-08 2011-08-11 Sangamo Biosciences, Inc. Engineered cleavage half-domains
WO2011100058A1 (en) 2010-02-09 2011-08-18 Sangamo Biosciences, Inc. Targeted genomic modification with partially single-stranded donor molecules
EP2563918B1 (en) * 2010-04-26 2016-11-23 Sangamo BioSciences, Inc. Genome editing of a rosa locus using zinc-finger nucleases
CN103025344B (zh) * 2010-05-17 2016-06-29 桑格摩生物科学股份有限公司 新型dna-结合蛋白及其用途
EP2392208B1 (en) 2010-06-07 2016-05-04 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Fusion proteins comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease and their use
EP2580331A4 (en) * 2010-06-14 2013-11-27 Univ Iowa State Res Found Inc NUCLEASE ACTIVITY OF THE TAL EFFECTOR AND FUSION PROTEIN FOKI
AU2018251150B2 (en) * 2017-04-13 2024-05-09 Albert-Ludwigs-Universität Freiburg New sequence specific reagents targeting CCR5 in primary hematopoietic cells

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