PT3216867T - Método para modificar a sequência de genoma para introduzir mutação específica a sequência de adn alvo por reação de remoção de bases, e complexo molecular nele utilizado - Google Patents
Método para modificar a sequência de genoma para introduzir mutação específica a sequência de adn alvo por reação de remoção de bases, e complexo molecular nele utilizadoInfo
- Publication number
- PT3216867T PT3216867T PT158573253T PT15857325T PT3216867T PT 3216867 T PT3216867 T PT 3216867T PT 158573253 T PT158573253 T PT 158573253T PT 15857325 T PT15857325 T PT 15857325T PT 3216867 T PT3216867 T PT 3216867T
- Authority
- PT
- Portugal
- Prior art keywords
- base
- molecular complex
- specific mutation
- complex used
- removal reaction
- Prior art date
Links
- 108091028043 Nucleic acid sequence Proteins 0.000 title 1
- 230000035772 mutation Effects 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
- C12Y302/02027—Uracil-DNA glycosylase (3.2.2.27)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2014224745 | 2014-11-04 |
Publications (1)
Publication Number | Publication Date |
---|---|
PT3216867T true PT3216867T (pt) | 2020-07-16 |
Family
ID=55909124
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PT158573253T PT3216867T (pt) | 2014-11-04 | 2015-11-02 | Método para modificar a sequência de genoma para introduzir mutação específica a sequência de adn alvo por reação de remoção de bases, e complexo molecular nele utilizado |
Country Status (8)
Country | Link |
---|---|
US (2) | US10920215B2 (pt) |
EP (2) | EP3216867B1 (pt) |
JP (1) | JP6153180B2 (pt) |
DK (1) | DK3216867T3 (pt) |
ES (1) | ES2800168T3 (pt) |
PL (1) | PL3216867T3 (pt) |
PT (1) | PT3216867T (pt) |
WO (1) | WO2016072399A1 (pt) |
Families Citing this family (43)
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JP6261500B2 (ja) | 2011-07-22 | 2018-01-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ヌクレアーゼ切断特異性の評価および改善 |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
US9840699B2 (en) | 2013-12-12 | 2017-12-12 | President And Fellows Of Harvard College | Methods for nucleic acid editing |
ES2752175T3 (es) | 2014-03-05 | 2020-04-03 | Univ Kobe Nat Univ Corp | Método de modificación de la secuencia genómica para convertir específicamente bases de ácido nucleico de una secuencia de ADN seleccionada como diana, y complejo molecular para su uso en el mismo |
WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
US10767173B2 (en) | 2015-09-09 | 2020-09-08 | National University Corporation Kobe University | Method for converting genome sequence of gram-positive bacterium by specifically converting nucleic acid base of targeted DNA sequence, and molecular complex used in same |
US20190225955A1 (en) | 2015-10-23 | 2019-07-25 | President And Fellows Of Harvard College | Evolved cas9 proteins for gene editing |
CN108495932B (zh) | 2015-11-27 | 2022-08-09 | 国立大学法人神户大学 | 用于特异性转换靶向dna序列的核酸碱基的单子叶植物的基因组序列的转换方法、及其使用的分子复合体 |
CA3021281C (en) * | 2016-04-21 | 2021-07-27 | National University Corporation Kobe University | Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor |
KR102547316B1 (ko) | 2016-08-03 | 2023-06-23 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
AU2017308889B2 (en) | 2016-08-09 | 2023-11-09 | President And Fellows Of Harvard College | Programmable Cas9-recombinase fusion proteins and uses thereof |
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US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
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US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
EP3592777A1 (en) * | 2017-03-10 | 2020-01-15 | President and Fellows of Harvard College | Cytosine to guanine base editor |
EP3604519A4 (en) * | 2017-03-22 | 2021-01-06 | National University Corporation Kobe University | METHOD OF NUCLEIC ACID SEQUENCE CONVERSION OF A CELL WITH SPECIFIC NUCLEIC ACID BASE CONVERSION OF TARGETED DNA USING ENDOGENIC DNA-MODIFYING ENZYME AND MOLECULAR COMPLEXES USED IN IT |
US11268082B2 (en) | 2017-03-23 | 2022-03-08 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
WO2019023680A1 (en) | 2017-07-28 | 2019-01-31 | President And Fellows Of Harvard College | METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE) |
WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
JP7328695B2 (ja) | 2017-11-22 | 2023-08-17 | 国立大学法人神戸大学 | 安定で副作用の少ないゲノム編集用複合体及びそれをコードする核酸 |
WO2019177684A1 (en) * | 2018-03-15 | 2019-09-19 | Massachusetts Institute Of Technology | Methods of quantifying rna and dna variants through sequencing employing phosphorothioates |
SG11202009319YA (en) * | 2018-03-26 | 2020-10-29 | Univ Kobe Nat Univ Corp | Method for modifying target site in double-stranded dna in cell |
KR20210041008A (ko) * | 2018-08-03 | 2021-04-14 | 빔 테라퓨틱스, 인크. | 핵산 표적 서열을 변형시키기 위한 다중-이펙터 핵염기 편집기 및 이를 이용하는 방법 |
US20210348178A1 (en) * | 2018-08-21 | 2021-11-11 | Pioneer Hi-Bred International, Inc. | Compositions and methods for modifying maturity in rice plants |
CA3130488A1 (en) | 2019-03-19 | 2020-09-24 | David R. Liu | Methods and compositions for editing nucleotide sequences |
WO2020229241A1 (en) | 2019-05-10 | 2020-11-19 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
US20220333133A1 (en) | 2019-09-03 | 2022-10-20 | Voyager Therapeutics, Inc. | Vectorized editing of nucleic acids to correct overt mutations |
EP4028530A1 (en) | 2019-09-12 | 2022-07-20 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
WO2021069387A1 (en) | 2019-10-07 | 2021-04-15 | Basf Se | Regulatory nucleic acid molecules for enhancing gene expression in plants |
CA3159805A1 (en) | 2019-12-03 | 2021-06-10 | Frank Meulewaeter | Regulatory nucleic acid molecules for enhancing gene expression in plants |
US20230040261A1 (en) * | 2020-03-11 | 2023-02-09 | North Carolina State University | Compositions, methods, and systems for genome editing technology |
GB2614813A (en) | 2020-05-08 | 2023-07-19 | Harvard College | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
WO2022158561A1 (ja) * | 2021-01-22 | 2022-07-28 | 国立大学法人東京大学 | 植物ゲノムの編集方法 |
JPWO2022181796A1 (pt) | 2021-02-26 | 2022-09-01 | ||
WO2024048599A1 (ja) * | 2022-08-31 | 2024-03-07 | 国立大学法人東京農工大学 | タンパク質-核酸複合体、これを用いた物質の検出キット及び検出方法 |
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GB9600384D0 (en) | 1996-01-09 | 1996-03-13 | Nyfotek As | Dna glycosylases |
WO1999010510A2 (en) * | 1997-08-26 | 1999-03-04 | Ariad Gene Therapeutics, Inc. | Fusion proteins comprising a dimerization, trimerization or tetramerization domain and an additional heterologous transcription activation, transcription repression, dna binding or ligand binding domain |
US8106255B2 (en) | 2002-01-23 | 2012-01-31 | Dana Carroll | Targeted chromosomal mutagenasis using zinc finger nucleases |
CN104531672B (zh) | 2007-03-02 | 2020-01-10 | 杜邦营养生物科学有限公司 | 具有改善的噬菌体抗性的培养物 |
BR112012014080A2 (pt) | 2009-12-10 | 2015-10-27 | Univ Iowa State Res Found | método para modificação do material genético, método para geração de um ácido nucleico, monômero de endonuclease efetora tal, método para geração de um aninal, método para geração de uma planta, método para recombinação genética direcionada, ácido nucleico, cassete de expressão e célula hospedeira |
JP2013128413A (ja) | 2010-03-11 | 2013-07-04 | Kyushu Univ | Pprモチーフを利用したrna結合性蛋白質の改変方法 |
US9040239B2 (en) * | 2012-03-15 | 2015-05-26 | New England Biolabs, Inc. | Composition and methods of oxygenation of nucleic acids containing 5-methylpyrimidine |
WO2014127287A1 (en) * | 2013-02-14 | 2014-08-21 | Massachusetts Institute Of Technology | Method for in vivo tergated mutagenesis |
RU2723130C2 (ru) * | 2013-04-05 | 2020-06-08 | ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи | Способы и композиции для встраивания экзогенной последовательности в геном растений |
JP2014224745A (ja) | 2013-05-16 | 2014-12-04 | 株式会社ミツトヨ | 原点信号発生装置及び原点信号発生システム |
US20150037790A1 (en) * | 2013-07-05 | 2015-02-05 | The University Of Southampton | Cytosine variant detection |
US9840699B2 (en) * | 2013-12-12 | 2017-12-12 | President And Fellows Of Harvard College | Methods for nucleic acid editing |
ES2752175T3 (es) | 2014-03-05 | 2020-04-03 | Univ Kobe Nat Univ Corp | Método de modificación de la secuencia genómica para convertir específicamente bases de ácido nucleico de una secuencia de ADN seleccionada como diana, y complejo molecular para su uso en el mismo |
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2015
- 2015-11-02 DK DK15857325.3T patent/DK3216867T3/da active
- 2015-11-02 WO PCT/JP2015/080958 patent/WO2016072399A1/ja active Application Filing
- 2015-11-02 EP EP15857325.3A patent/EP3216867B1/en active Active
- 2015-11-02 PL PL15857325T patent/PL3216867T3/pl unknown
- 2015-11-02 PT PT158573253T patent/PT3216867T/pt unknown
- 2015-11-02 JP JP2016516997A patent/JP6153180B2/ja active Active
- 2015-11-02 EP EP20168727.4A patent/EP3739047A1/en active Pending
- 2015-11-02 ES ES15857325T patent/ES2800168T3/es active Active
- 2015-11-02 US US15/523,939 patent/US10920215B2/en active Active
-
2021
- 2021-02-12 US US17/175,245 patent/US20210171935A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US20210171935A1 (en) | 2021-06-10 |
EP3216867A1 (en) | 2017-09-13 |
WO2016072399A1 (ja) | 2016-05-12 |
US20170321210A1 (en) | 2017-11-09 |
US10920215B2 (en) | 2021-02-16 |
DK3216867T3 (da) | 2020-06-22 |
ES2800168T3 (es) | 2020-12-28 |
EP3216867A4 (en) | 2018-04-04 |
EP3216867B1 (en) | 2020-04-15 |
PL3216867T3 (pl) | 2020-11-02 |
EP3739047A1 (en) | 2020-11-18 |
JP6153180B2 (ja) | 2017-06-28 |
JPWO2016072399A1 (ja) | 2017-04-27 |
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