DK3359671T3 - Dna-vektorer, transposoner og transposaser til eukaryot genommodifikation - Google Patents
Dna-vektorer, transposoner og transposaser til eukaryot genommodifikation Download PDFInfo
- Publication number
- DK3359671T3 DK3359671T3 DK16854354.4T DK16854354T DK3359671T3 DK 3359671 T3 DK3359671 T3 DK 3359671T3 DK 16854354 T DK16854354 T DK 16854354T DK 3359671 T3 DK3359671 T3 DK 3359671T3
- Authority
- DK
- Denmark
- Prior art keywords
- eukaryot
- transposases
- transposons
- dna vectors
- genome modification
- Prior art date
Links
- 241000206602 Eukaryota Species 0.000 title 1
- 102000008579 Transposases Human genes 0.000 title 1
- 108010020764 Transposases Proteins 0.000 title 1
- 230000004048 modification Effects 0.000 title 1
- 238000012986 modification Methods 0.000 title 1
- 239000013598 vector Substances 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/165—Yeast isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/007—Vector systems having a special element relevant for transcription cell cycle specific enhancer/promoter combination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/40—Vector systems having a special element relevant for transcription being an insulator
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/84—Pichia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562239109P | 2015-10-08 | 2015-10-08 | |
US201662325872P | 2016-04-21 | 2016-04-21 | |
US201662373422P | 2016-08-11 | 2016-08-11 | |
PCT/US2016/055824 WO2017062668A2 (en) | 2015-10-08 | 2016-10-06 | Dna vectors, transposons and transposases for eukaryotic genome modification |
Publications (1)
Publication Number | Publication Date |
---|---|
DK3359671T3 true DK3359671T3 (da) | 2021-09-20 |
Family
ID=58488526
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK16854354.4T DK3359671T3 (da) | 2015-10-08 | 2016-10-06 | Dna-vektorer, transposoner og transposaser til eukaryot genommodifikation |
Country Status (8)
Country | Link |
---|---|
US (6) | US10435696B2 (da) |
EP (2) | EP3359671B1 (da) |
CA (1) | CA3001312A1 (da) |
DK (1) | DK3359671T3 (da) |
ES (1) | ES2891087T3 (da) |
HU (1) | HUE056009T2 (da) |
LT (1) | LT3359671T (da) |
WO (1) | WO2017062668A2 (da) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10041077B2 (en) | 2014-04-09 | 2018-08-07 | Dna2.0, Inc. | DNA vectors, transposons and transposases for eukaryotic genome modification |
EP3359671B1 (en) | 2015-10-08 | 2021-08-18 | Dna Twopointo Inc. | Dna vectors, transposons and transposases for eukaryotic genome modification |
EP3612623B1 (en) | 2017-05-06 | 2024-07-03 | Upside Foods, Inc. | Compositions and methods for increasing the culture density of a cellular biomass within a cultivation infrastructure |
US11479792B2 (en) | 2017-07-13 | 2022-10-25 | Upside Foods, Inc. | Compositions and methods for increasing the efficiency of cell cultures used for food production |
JP2022520283A (ja) * | 2019-02-19 | 2022-03-29 | ヨーロピアン モレキュラー バイオロジー ラボラトリー | 細胞透過性トランスポザーゼ |
AU2020232850A1 (en) | 2019-03-07 | 2021-10-07 | The Trustees Of Columbia University In The City Of New York | RNA-guided DNA integration using Tn7-like transposons |
EP3953480A4 (en) * | 2019-04-08 | 2023-01-11 | Dna Twopointo Inc. | TRANSPOSITION OF NUCLEIC ACIDS INTO EUKARYOTIC GENOMES WITH A TRANSPOSASE FROM HELIOTHIS |
AU2020272667B2 (en) | 2019-04-08 | 2023-07-06 | Dna Twopointo Inc. | Transposition of nucleic acid constructs into eukaryotic genomes with a transposase from amyelois |
KR20210143897A (ko) * | 2019-04-08 | 2021-11-29 | 디앤에이 투포인토 인크. | 오리지아스로부터의 트랜스포사제를 이용한 핵산 작제물의 진핵세포로의 통합 |
EP4107272A2 (en) * | 2020-02-19 | 2022-12-28 | Wuxi Biologics Ireland Limited | Enhanced expression system and methods of use thereof |
CA3173889A1 (en) | 2020-05-04 | 2021-11-11 | Saliogen Therapeutics, Inc. | Transposition-based therapies |
JP7419168B2 (ja) * | 2020-06-10 | 2024-01-22 | 株式会社東芝 | 改変型piggyBacトランスポゼースのポリペプチド、それをコードするポリヌクレオチド、導入キャリア、キット、細胞のゲノムに目的配列を組込む方法及び細胞製造方法 |
WO2024055013A1 (en) * | 2022-09-08 | 2024-03-14 | Metagenomi, Inc. | Systems and methods for transposing cargo nucleotide sequences |
Family Cites Families (46)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ZA973367B (en) | 1996-04-19 | 1997-11-18 | Innogenetics Nv | Method for typing and detecting HBV. |
US6136597A (en) | 1997-09-18 | 2000-10-24 | The Salk Institute For Biological Studies | RNA export element |
US6897066B1 (en) | 1997-09-26 | 2005-05-24 | Athersys, Inc. | Compositions and methods for non-targeted activation of endogenous genes |
DE19848017A1 (de) * | 1998-10-17 | 2000-04-20 | Multigene Biotech Gmbh | Episomal replizierender Vektor zur Expression eines Transgens in Säugerzellen |
CA2394142A1 (en) | 1999-12-06 | 2001-06-07 | Institut Pasteur | Isolated polynucleotides having a reduced or an increased content of epigenetic control motifs and uses thereof |
US20030150007A1 (en) | 2000-03-21 | 2003-08-07 | Charalambos Savakis | Method of generating transgenic organisms using transposons |
WO2002002783A2 (en) | 2000-07-03 | 2002-01-10 | Gala Design, Inc. | Expression vectors |
US20030143740A1 (en) * | 2001-10-15 | 2003-07-31 | Christine Wooddell | Processes for transposase mediated integration into mammalian cells |
FR2832424B1 (fr) | 2001-11-20 | 2004-09-24 | Genethon Iii | Plasmide chimere comprenant un genome retroviral replicatif et utilisations |
AU2003278695A1 (en) * | 2002-05-28 | 2004-02-02 | Maxygen, Inc. | Nucleic acid vectors |
WO2004065581A2 (en) * | 2003-01-15 | 2004-08-05 | Discovery Genomics, Inc. | Transposon-insulator element delivery systems |
US8005620B2 (en) | 2003-08-01 | 2011-08-23 | Dna Twopointo Inc. | Systems and methods for biopolymer engineering |
TW200613554A (en) | 2004-06-17 | 2006-05-01 | Wyeth Corp | Plasmid having three complete transcriptional units and immunogenic compositions for inducing an immune response to HIV |
US7910101B2 (en) * | 2004-10-25 | 2011-03-22 | Centocor, Inc. | Melanocortin receptor binding mimetibodies, compositions, methods and uses |
WO2006096989A2 (en) | 2005-03-17 | 2006-09-21 | National Research Council Of Canada | Expression vectors containing a truncated epstein barr nuclear antigen 1 lacking the gly-gly-ala domain for enhanced transient gene expression |
WO2006122442A1 (en) | 2005-05-14 | 2006-11-23 | Fudan University | Piggybac as a tool for genetic manipulation and analysis in vertebrates |
WO2006124001A1 (en) * | 2005-05-17 | 2006-11-23 | Temasek Life Sciences Laboratory Limited | Transposition of maize ac/ds elements in vertebrates |
TWI300441B (en) | 2005-09-21 | 2008-09-01 | Univ Chung Yuan Christian | A polynucleotide with ires activity |
US20070204356A1 (en) | 2006-02-28 | 2007-08-30 | University Of Notre Dame Du Lac | PiggyBac constructs in vertebrates |
CA2656598A1 (en) | 2006-08-07 | 2008-02-21 | Avigenics, Inc. | Hybrid promoters |
EP2520935A3 (en) * | 2006-08-09 | 2013-02-13 | Homestead Clinical Corporation | Organ-specific proteins and methods of their use |
US8546643B2 (en) * | 2007-03-07 | 2013-10-01 | Aarhus Universitet | Pig model for atherosclerosis |
US20110130444A1 (en) | 2007-05-04 | 2011-06-02 | Stefan Moisyadi | Methods and compositions for targeted delivery of gene therapeutic vectors |
US20090042297A1 (en) | 2007-06-01 | 2009-02-12 | George Jr Alfred L | Piggybac transposon-based vectors and methods of nucleic acid integration |
US9228180B2 (en) * | 2007-07-04 | 2016-01-05 | Max-Delbruck-Centrum Fur Molekulare Medizin | Polypeptide variants of sleeping beauty transposase |
US8309791B2 (en) * | 2008-07-16 | 2012-11-13 | Recombinectics, Inc. | Method for producing a transgenic pig using a hyper-methylated transposon |
GB0903207D0 (en) | 2009-02-25 | 2009-04-08 | Ucb Pharma Sa | Method for expressing multimeric proteins |
WO2010099301A2 (en) * | 2009-02-25 | 2010-09-02 | The Johns Hopkins University | Piggybac transposon variants and methods of use |
WO2010099296A1 (en) * | 2009-02-26 | 2010-09-02 | Transposagen Biopharmaceuticals, Inc. | Hyperactive piggybac transposases |
US8592211B2 (en) * | 2009-03-20 | 2013-11-26 | The Rockefeller University | Enhanced PiggyBac transposon and methods for transposon mutagenesis |
WO2011016588A1 (en) | 2009-08-07 | 2011-02-10 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
US9790489B2 (en) | 2009-10-26 | 2017-10-17 | Chang Gung University | Method for performing genetic modification under a drug-free environment and components thereof |
WO2011101696A1 (en) * | 2010-02-18 | 2011-08-25 | Cellectis | Improved meganuclease recombination system |
EP3118316A1 (en) * | 2010-09-02 | 2017-01-18 | Université de Mons | Agents useful in treating facioscapulohumeral muscular dystrophy |
WO2012074758A1 (en) * | 2010-11-16 | 2012-06-07 | Transposagen Bioharmaceuticals, Inc. | Hyperactive piggybac transposases |
US9783790B2 (en) * | 2011-07-15 | 2017-10-10 | The Johns Hopkins University | Trichoplusia ni piggybac transposases with reduced integration activity |
WO2013173635A1 (en) * | 2012-05-16 | 2013-11-21 | Rana Therapeutics, Inc. | Compositions and methods for modulating gene expression |
CN113151179A (zh) | 2012-07-31 | 2021-07-23 | 阿格克斯治疗有限公司 | Hla g修饰的细胞及方法 |
US8975042B2 (en) * | 2012-12-21 | 2015-03-10 | Dna Twopointo, Inc. | Fluorescent and colored proteins and methods for using them |
US20160002330A1 (en) * | 2013-02-13 | 2016-01-07 | Laboratoire Francais Du Fractionnement Et Des Biotechnologies | Cetuximab with modified glycosylation and uses thereof |
WO2014140218A1 (en) | 2013-03-13 | 2014-09-18 | Fundación Pública Andaluza Progreso Y Salud | Insulator to improve gene transfer vectors |
AU2014315287A1 (en) * | 2013-09-03 | 2015-03-12 | Moderna Therapeutics, Inc. | Chimeric polynucleotides |
CA2939541C (en) * | 2014-02-14 | 2022-11-08 | Symvivo Corporation | Hybrid proteins and uses thereof |
US10041077B2 (en) | 2014-04-09 | 2018-08-07 | Dna2.0, Inc. | DNA vectors, transposons and transposases for eukaryotic genome modification |
DK3129487T3 (da) | 2014-04-09 | 2020-11-30 | Dna Twopointo Inc | Forbedrede nukleinsyrekonstruktioner til eukaryot genekspression |
EP3359671B1 (en) | 2015-10-08 | 2021-08-18 | Dna Twopointo Inc. | Dna vectors, transposons and transposases for eukaryotic genome modification |
-
2016
- 2016-10-06 EP EP16854354.4A patent/EP3359671B1/en active Active
- 2016-10-06 HU HUE16854354A patent/HUE056009T2/hu unknown
- 2016-10-06 CA CA3001312A patent/CA3001312A1/en active Pending
- 2016-10-06 EP EP21191240.7A patent/EP3974524A1/en active Pending
- 2016-10-06 ES ES16854354T patent/ES2891087T3/es active Active
- 2016-10-06 WO PCT/US2016/055824 patent/WO2017062668A2/en active Application Filing
- 2016-10-06 DK DK16854354.4T patent/DK3359671T3/da active
- 2016-10-06 LT LTEPPCT/US2016/055824T patent/LT3359671T/lt unknown
-
2018
- 2018-05-24 US US15/989,124 patent/US10435696B2/en active Active
- 2018-09-24 US US16/140,433 patent/US20190010505A1/en not_active Abandoned
-
2019
- 2019-02-20 US US16/280,676 patent/US20190169622A1/en not_active Abandoned
- 2019-06-20 US US16/447,778 patent/US20190323018A1/en not_active Abandoned
- 2019-12-23 US US16/726,163 patent/US20200190524A1/en active Pending
-
2021
- 2021-01-15 US US17/150,878 patent/US20210139919A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US20200190524A1 (en) | 2020-06-18 |
WO2017062668A3 (en) | 2017-08-03 |
US20190169622A1 (en) | 2019-06-06 |
US10435696B2 (en) | 2019-10-08 |
CA3001312A1 (en) | 2017-04-13 |
WO2017062668A2 (en) | 2017-04-13 |
EP3359671A2 (en) | 2018-08-15 |
HUE056009T2 (hu) | 2022-01-28 |
ES2891087T3 (es) | 2022-01-26 |
US20190323018A1 (en) | 2019-10-24 |
US20180258436A1 (en) | 2018-09-13 |
EP3359671B1 (en) | 2021-08-18 |
EP3974524A1 (en) | 2022-03-30 |
EP3359671A4 (en) | 2019-03-20 |
US20210139919A1 (en) | 2021-05-13 |
LT3359671T (lt) | 2021-12-10 |
US20190010505A1 (en) | 2019-01-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK3359671T3 (da) | Dna-vektorer, transposoner og transposaser til eukaryot genommodifikation | |
HK1248284A1 (zh) | 多重乳液核酸擴增 | |
IL261327A (en) | Trans duplication of RNA | |
DK3348636T3 (da) | Fremgangsmåde til modificering af en genomsekvens, der specifikt omdanner nukleobasen af en targetteret DNA-sekvens, og molekylær sekvens anvendt i fremgangsmåden | |
DK3456846T3 (da) | Systemer og fremgangsmåder til at stregkodemarkere nukleinsyre | |
DK3325669T3 (da) | Sammensætninger og fremgangsmåder til RNA-analyse | |
DK3299923T3 (da) | Fremgangsmåde til at styre gåstilling af tobenet robot og indretning | |
DK3215601T3 (da) | Fremgangsmåder til transduktion og celleforarbejdning | |
DK3108009T3 (da) | Fremgangsmåder og sammensætninger til DNA-profilering | |
DK3161751T3 (da) | System, indretning og fremgangsmåde til robotiseret objekthåndtering | |
DK3122870T3 (da) | Fremgangsmåder og genetiske systemer til celleteknik | |
GB201519584D0 (en) | Nucleic acids, peptides and methods | |
SG10201913820TA (en) | Nucleic Acid Analysis Apparatus | |
ZA201801947B (en) | Compounds as dna probes, methods and applications thereof | |
DK3234160T3 (da) | Sammensætninger og fremgangsmåder til hjælper-stamme-medieret svampegenommodifikation | |
GB201510723D0 (en) | High throughput isothermal nucleic acid amplification | |
SG10201600867XA (en) | Nucleic acid extracting device | |
DK3222717T3 (da) | Fremgangsmåde til at amplificere cirkulært dna | |
GB201408841D0 (en) | Nucleic acid processing | |
DK3350343T3 (da) | Fremgangsmåder og sammensætninger til genommålberigelse og selektiv dna-sekventering | |
HK1250682A1 (zh) | 核酸擴增 | |
GB201717445D0 (en) | Method for determining nucleotide sequence | |
GB201517241D0 (en) | DNA modification | |
GB201415674D0 (en) | Nucleic acid analysis | |
DK3225616T3 (da) | Alpha-asary-laldehydester, fremgangsmåde til fremstilling deraf og anvendelse deraf |