DK3222717T3 - Fremgangsmåde til at amplificere cirkulært dna - Google Patents
Fremgangsmåde til at amplificere cirkulært dna Download PDFInfo
- Publication number
- DK3222717T3 DK3222717T3 DK15860324.1T DK15860324T DK3222717T3 DK 3222717 T3 DK3222717 T3 DK 3222717T3 DK 15860324 T DK15860324 T DK 15860324T DK 3222717 T3 DK3222717 T3 DK 3222717T3
- Authority
- DK
- Denmark
- Prior art keywords
- procedure
- circular dna
- amplifying circular
- amplifying
- dna
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2014233563 | 2014-11-18 | ||
PCT/JP2015/082356 WO2016080424A1 (ja) | 2014-11-18 | 2015-11-18 | 環状dnaの増幅方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
DK3222717T3 true DK3222717T3 (da) | 2020-06-29 |
Family
ID=56013959
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK15860324.1T DK3222717T3 (da) | 2014-11-18 | 2015-11-18 | Fremgangsmåde til at amplificere cirkulært dna |
Country Status (7)
Country | Link |
---|---|
US (2) | US10301672B2 (da) |
EP (1) | EP3222717B1 (da) |
JP (1) | JP6262877B2 (da) |
CN (1) | CN107002069A (da) |
DK (1) | DK3222717T3 (da) |
ES (1) | ES2788726T3 (da) |
WO (1) | WO2016080424A1 (da) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6262877B2 (ja) | 2014-11-18 | 2018-01-24 | 国立研究開発法人科学技術振興機構 | 環状dnaの増幅方法 |
EP3460058B1 (en) * | 2016-05-17 | 2024-03-06 | Moderna Enzymatics Co., Ltd. | Method for amplifying cyclic dna |
AU2018228154A1 (en) | 2017-02-28 | 2019-09-26 | OriCiro Genomics, Inc. | Method for replicating or amplifying circular DNA |
CA3068615C (en) * | 2017-07-05 | 2023-09-05 | OriCiro Genomics, Inc. | Dna production method and dna fragment-joining kit |
US10174295B1 (en) * | 2017-08-01 | 2019-01-08 | The Charles Stark Draper Laboratory, Inc. | Composition of matter: engineering of Escherichia coli phage K1E |
RU2766717C1 (ru) * | 2018-07-30 | 2022-03-15 | Орикиро Дженомикс, Инк. | Способ редактирования днк в бесклеточной системе |
US20220025460A1 (en) * | 2020-07-21 | 2022-01-27 | The University Of Tokyo | Method and kit for determining neuromuscular disease in subject |
TW202338094A (zh) * | 2022-01-19 | 2023-10-01 | 日商奥利希羅基因組學有限公司 | 功能性dna卡匣及質體 |
WO2023164650A1 (en) | 2022-02-24 | 2023-08-31 | The Jackson Laboratories | Ori-c mediated extrachromosomal dna amplication and sequencing for diagnosing, screening and treating diseases |
WO2023191034A1 (ja) * | 2022-03-31 | 2023-10-05 | モデルナ・エンザイマティクス株式会社 | 配列エラーの減少した二本鎖dnaの製造方法 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
US6355450B1 (en) | 1995-04-21 | 2002-03-12 | Human Genome Sciences, Inc. | Computer readable genomic sequence of Haemophilus influenzae Rd, fragments thereof, and uses thereof |
US7279313B2 (en) * | 1995-09-15 | 2007-10-09 | Centelion | Circular DNA molecule having a conditional origin of replication, process for their preparation and their use in gene therapy |
US5925545A (en) * | 1996-09-09 | 1999-07-20 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
EP1165054A4 (en) | 1999-04-06 | 2005-02-09 | Pharmaquest Ltd | PHARMACEUTICAL DOSAGE FORM FOR INTERMITTENT DELIVERY OF METHYLPHENIDATE |
KR20020011139A (ko) | 1999-06-18 | 2002-02-07 | 게리 디. 스트리트, 스티븐 엘. 네스비트 | 저 복제수 플라스미드의 클로닝 및 발현을 개선시키기위한 벡터 |
AU6731300A (en) * | 1999-10-05 | 2001-05-10 | Agene Research Institute Co., Ltd. | Nuclear localized recq5 type dna helicase |
JP4214230B2 (ja) | 2004-02-23 | 2009-01-28 | 独立行政法人農業・食品産業技術総合研究機構 | 環状dnaへのランダム変異導入方法 |
EP1759008A4 (en) * | 2004-04-26 | 2008-08-06 | Replidyne Inc | SYSTEMS AND METHODS FOR BACTERIAL REPLICATION |
US20080096258A1 (en) | 2006-10-24 | 2008-04-24 | Christian Korfhage | Rolling circle amplification of circular genomes |
US8921072B2 (en) | 2008-09-02 | 2014-12-30 | General Electric Compnay | Methods to generate DNA mini-circles |
JP6262877B2 (ja) | 2014-11-18 | 2018-01-24 | 国立研究開発法人科学技術振興機構 | 環状dnaの増幅方法 |
EP3460058B1 (en) | 2016-05-17 | 2024-03-06 | Moderna Enzymatics Co., Ltd. | Method for amplifying cyclic dna |
AU2018228154A1 (en) | 2017-02-28 | 2019-09-26 | OriCiro Genomics, Inc. | Method for replicating or amplifying circular DNA |
-
2015
- 2015-11-18 JP JP2016560254A patent/JP6262877B2/ja active Active
- 2015-11-18 US US15/527,560 patent/US10301672B2/en active Active
- 2015-11-18 CN CN201580063334.6A patent/CN107002069A/zh active Pending
- 2015-11-18 EP EP15860324.1A patent/EP3222717B1/en active Active
- 2015-11-18 DK DK15860324.1T patent/DK3222717T3/da active
- 2015-11-18 ES ES15860324T patent/ES2788726T3/es active Active
- 2015-11-18 WO PCT/JP2015/082356 patent/WO2016080424A1/ja active Application Filing
-
2019
- 2019-04-01 US US16/371,766 patent/US11319579B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
ES2788726T3 (es) | 2020-10-22 |
EP3222717A4 (en) | 2018-04-25 |
CN107002069A (zh) | 2017-08-01 |
JP6262877B2 (ja) | 2018-01-24 |
WO2016080424A1 (ja) | 2016-05-26 |
EP3222717A1 (en) | 2017-09-27 |
US20190249236A1 (en) | 2019-08-15 |
EP3222717B1 (en) | 2020-04-22 |
JPWO2016080424A1 (ja) | 2017-09-07 |
US10301672B2 (en) | 2019-05-28 |
US11319579B2 (en) | 2022-05-03 |
US20170321263A1 (en) | 2017-11-09 |
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