SG11202009319YA - Method for modifying target site in double-stranded dna in cell - Google Patents

Method for modifying target site in double-stranded dna in cell

Info

Publication number
SG11202009319YA
SG11202009319YA SG11202009319YA SG11202009319YA SG11202009319YA SG 11202009319Y A SG11202009319Y A SG 11202009319YA SG 11202009319Y A SG11202009319Y A SG 11202009319YA SG 11202009319Y A SG11202009319Y A SG 11202009319YA SG 11202009319Y A SG11202009319Y A SG 11202009319YA
Authority
SG
Singapore
Prior art keywords
double
cell
target site
stranded dna
modifying target
Prior art date
Application number
SG11202009319YA
Inventor
Keiji Nishida
Original Assignee
Univ Kobe Nat Univ Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kobe Nat Univ Corp filed Critical Univ Kobe Nat Univ Corp
Publication of SG11202009319YA publication Critical patent/SG11202009319YA/en

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11202009319YA 2018-03-26 2019-03-26 Method for modifying target site in double-stranded dna in cell SG11202009319YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2018059073 2018-03-26
PCT/JP2019/012807 WO2019189147A1 (en) 2018-03-26 2019-03-26 Method for modifying target site in double-stranded dna in cell

Publications (1)

Publication Number Publication Date
SG11202009319YA true SG11202009319YA (en) 2020-10-29

Family

ID=68061818

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202009319YA SG11202009319YA (en) 2018-03-26 2019-03-26 Method for modifying target site in double-stranded dna in cell

Country Status (9)

Country Link
US (1) US11041169B2 (en)
EP (1) EP3660152A4 (en)
JP (3) JP6727680B2 (en)
KR (1) KR102287880B1 (en)
CN (1) CN111148833B (en)
BR (1) BR112020019301A2 (en)
CA (1) CA3095291C (en)
SG (1) SG11202009319YA (en)
WO (1) WO2019189147A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4209589A1 (en) 2020-09-04 2023-07-12 National University Corporation Kobe University Miniaturized cytidine deaminase-containing complex for modifying double-stranded dna
CN115725650A (en) * 2021-08-26 2023-03-03 华东师范大学 Base editing system for realizing A to C and/or A to T base mutation and application thereof

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003087341A2 (en) 2002-01-23 2003-10-23 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
WO2010012077A1 (en) 2008-07-28 2010-02-04 Mount Sinai Hospital Compositions, methods and kits for reprogramming somatic cells
HUE041436T2 (en) 2009-12-10 2019-05-28 Univ Minnesota Tal effector-mediated DNA modification
JP2013128413A (en) 2010-03-11 2013-07-04 Kyushu Univ Method for modifying rna-binding protein using ppr motif
TR201806812T4 (en) * 2012-05-25 2018-06-21 Charpentier Emmanuelle Methods and compositions for RNA-directed target DNA modification and for RNA-directed transcription modification.
US20150044772A1 (en) 2013-08-09 2015-02-12 Sage Labs, Inc. Crispr/cas system-based novel fusion protein and its applications in genome editing
DK3115457T3 (en) 2014-03-05 2019-11-04 Univ Kobe Nat Univ Corp PROCEDURE FOR MODIFYING GENE SEQUENCE TO SPECIFICALLY CONVERT THE NUCLEIC ACID BASES OF TARGETED DNA SEQUENCE AND MOLECULAR COMPLEX TO USE IN SAME
ES2800168T3 (en) 2014-11-04 2020-12-28 Univ Kobe Nat Univ Corp Procedure for modifying a genomic sequence to introduce a specific mutation in a target DNA sequence by base elimination reaction, and the molecular complex used in it
DK3348636T3 (en) 2015-09-09 2022-02-28 Univ Kobe Nat Univ Corp Method for modifying a genome sequence that specifically converts the nucleobase of a targeted DNA sequence and molecular sequence used in the method
US10767173B2 (en) 2015-09-09 2020-09-08 National University Corporation Kobe University Method for converting genome sequence of gram-positive bacterium by specifically converting nucleic acid base of targeted DNA sequence, and molecular complex used in same
WO2017053312A1 (en) 2015-09-21 2017-03-30 The Regents Of The University Of California Compositions and methods for target nucleic acid modification
IL310721A (en) * 2015-10-23 2024-04-01 Harvard College Nucleobase editors and uses thereof
BR112018010681A8 (en) 2015-11-27 2019-02-26 Univ Kobe Nat Univ Corp method for modifying a targeted site, nucleic acid modification enzyme complex, and nucleic acid.
JP6907856B2 (en) 2016-10-04 2021-07-21 信越化学工業株式会社 Copolymer of (meth) acrylic acid triisopropylsilyl and (meth) acrylic acid derivative and method for producing the same
KR102541398B1 (en) 2017-06-08 2023-06-07 고꾸리쯔 다이가꾸 호우징 오사까 다이가꾸 Method for producing DNA-edited eukaryotic cells and kits used in the method
JP7212943B2 (en) * 2017-06-16 2023-01-26 国立大学法人 長崎大学 Transcriptional regulatory regions of oncogenes

Also Published As

Publication number Publication date
CN111148833B (en) 2021-04-02
KR102287880B1 (en) 2021-08-09
JP2023063448A (en) 2023-05-09
US20200270631A1 (en) 2020-08-27
EP3660152A4 (en) 2020-07-15
US11041169B2 (en) 2021-06-22
JP2020191879A (en) 2020-12-03
JPWO2019189147A1 (en) 2020-04-30
BR112020019301A2 (en) 2021-01-05
CN111148833A (en) 2020-05-12
EP3660152A1 (en) 2020-06-03
CA3095291C (en) 2022-10-11
CA3095291A1 (en) 2019-10-03
JP6727680B2 (en) 2020-07-22
JP7250349B2 (en) 2023-04-03
KR20200123255A (en) 2020-10-28
WO2019189147A1 (en) 2019-10-03

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