HK1253544A1 - 通過特異性地轉化靶dna序列的核碱基轉化革蘭氏陽性細菌的基因組序列的方法,以及用於該方法的分子複合物 - Google Patents
通過特異性地轉化靶dna序列的核碱基轉化革蘭氏陽性細菌的基因組序列的方法,以及用於該方法的分子複合物Info
- Publication number
- HK1253544A1 HK1253544A1 HK18112746.2A HK18112746A HK1253544A1 HK 1253544 A1 HK1253544 A1 HK 1253544A1 HK 18112746 A HK18112746 A HK 18112746A HK 1253544 A1 HK1253544 A1 HK 1253544A1
- Authority
- HK
- Hong Kong
- Prior art keywords
- converting
- gram
- nucleic acid
- same
- acid base
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title 1
- 108091028043 Nucleic acid sequence Proteins 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04005—Cytidine deaminase (3.5.4.5)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015178022 | 2015-09-09 |
Publications (1)
Publication Number | Publication Date |
---|---|
HK1253544A1 true HK1253544A1 (zh) | 2019-06-21 |
Family
ID=58239975
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HK18112746.2A HK1253544A1 (zh) | 2015-09-09 | 2018-10-08 | 通過特異性地轉化靶dna序列的核碱基轉化革蘭氏陽性細菌的基因組序列的方法,以及用於該方法的分子複合物 |
Country Status (9)
Country | Link |
---|---|
US (1) | US10767173B2 (zh) |
EP (1) | EP3348638B1 (zh) |
JP (1) | JP6664693B2 (zh) |
CN (1) | CN108271384B (zh) |
BR (1) | BR112018004636A2 (zh) |
DK (1) | DK3348638T3 (zh) |
ES (1) | ES2938623T3 (zh) |
HK (1) | HK1253544A1 (zh) |
WO (1) | WO2017043656A1 (zh) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2853829C (en) | 2011-07-22 | 2023-09-26 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
GB201315475D0 (en) | 2013-08-30 | 2013-10-16 | Green Biologics Ltd | Solvent production |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
IL294014B2 (en) | 2015-10-23 | 2024-07-01 | Harvard College | Nucleobase editors and their uses |
DK3382019T3 (da) | 2015-11-27 | 2022-05-30 | Univ Kobe Nat Univ Corp | Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri |
DK3447139T3 (da) * | 2016-04-21 | 2022-07-04 | Univ Kobe Nat Univ Corp | Fremgangsmåde til øgning af mutationsindføringseffektivitet i genomsekvensmodificeringsteknik, og molekylært kompleks til anvendelse dertil |
IL308426A (en) | 2016-08-03 | 2024-01-01 | Harvard College | Adenosine nuclear base editors and their uses |
US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
EP3592777A1 (en) | 2017-03-10 | 2020-01-15 | President and Fellows of Harvard College | Cytosine to guanine base editor |
JP7191388B2 (ja) | 2017-03-23 | 2022-12-19 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸によってプログラム可能なdna結合蛋白質を含む核酸塩基編集因子 |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
SG11202009319YA (en) | 2018-03-26 | 2020-10-29 | Univ Kobe Nat Univ Corp | Method for modifying target site in double-stranded dna in cell |
JP7126403B2 (ja) * | 2018-08-06 | 2022-08-26 | 株式会社日本触媒 | 遺伝子改変クロストリジウム・サッカロパーブチルアセトニカム種微生物 |
KR20210040985A (ko) * | 2018-08-07 | 2021-04-14 | 가부시키가이샤 모달리스 | 신규 전사 액티베이터 |
WO2020191243A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
CN110343689B (zh) * | 2019-08-23 | 2021-11-05 | 四川大学 | 一种链霉菌胰蛋白酶gm2938及其在枯草芽孢杆菌中的异源表达 |
US20220333133A1 (en) | 2019-09-03 | 2022-10-20 | Voyager Therapeutics, Inc. | Vectorized editing of nucleic acids to correct overt mutations |
DE112021002672T5 (de) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz |
Family Cites Families (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2003251286B2 (en) | 2002-01-23 | 2007-08-16 | The University Of Utah Research Foundation | Targeted chromosomal mutagenesis using zinc finger nucleases |
TR201905633T4 (tr) | 2007-03-02 | 2019-05-21 | Dupont Nutrition Biosci Aps | İyileştirilmiş faj direnci olan kültürler. |
WO2010132092A2 (en) | 2009-05-12 | 2010-11-18 | The Scripps Research Institute | Cytidine deaminase fusions and related methods |
US20110104787A1 (en) * | 2009-11-05 | 2011-05-05 | President And Fellows Of Harvard College | Fusion Peptides That Bind to and Modify Target Nucleic Acid Sequences |
US8586363B2 (en) * | 2009-12-10 | 2013-11-19 | Regents Of The University Of Minnesota | TAL effector-mediated DNA modification |
JP2013128413A (ja) | 2010-03-11 | 2013-07-04 | Kyushu Univ | Pprモチーフを利用したrna結合性蛋白質の改変方法 |
EP2784157B1 (en) | 2011-10-21 | 2019-09-18 | Kyushu University, National University Corporation | Design method for rna-binding protein using ppr motif, and use thereof |
GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
EP2828384B1 (en) * | 2012-03-23 | 2018-05-09 | Cellectis | Method to overcome dna chemical modifications sensitivity of engineered tale dna binding domains |
CN104471067B (zh) * | 2012-05-07 | 2020-08-14 | 桑格摩生物治疗股份有限公司 | 用于核酸酶介导的转基因靶向整合的方法和组合物 |
DK2800811T3 (en) | 2012-05-25 | 2017-07-17 | Univ Vienna | METHODS AND COMPOSITIONS FOR RNA DIRECTIVE TARGET DNA MODIFICATION AND FOR RNA DIRECTIVE MODULATION OF TRANSCRIPTION |
US20150315576A1 (en) | 2012-11-01 | 2015-11-05 | Massachusetts Institute Of Technology | Genetic device for the controlled destruction of dna |
EP4286402A3 (en) | 2012-12-12 | 2024-02-14 | The Broad Institute, Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
KR20150105633A (ko) | 2012-12-12 | 2015-09-17 | 더 브로드 인스티튜트, 인코퍼레이티드 | 서열 조작을 위한 시스템, 방법 및 최적화된 가이드 조성물의 조작 |
KR102192599B1 (ko) | 2013-04-05 | 2020-12-18 | 다우 아그로사이언시즈 엘엘씨 | 식물의 게놈 내의 외인성 서열의 통합을 위한 방법 및 조성물 |
US9873907B2 (en) | 2013-05-29 | 2018-01-23 | Agilent Technologies, Inc. | Method for fragmenting genomic DNA using CAS9 |
US9737604B2 (en) * | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
CN111471675A (zh) | 2014-03-05 | 2020-07-31 | 国立大学法人神户大学 | 特异性转变靶向dna序列的核酸碱基的基因组序列的修饰方法、及其使用的分子复合体 |
JP6153180B2 (ja) | 2014-11-04 | 2017-06-28 | 国立大学法人神戸大学 | 脱塩基反応により標的化したdna配列に特異的に変異を導入する、ゲノム配列の改変方法、並びにそれに用いる分子複合体 |
JP6780860B2 (ja) | 2015-09-09 | 2020-11-04 | 国立大学法人神戸大学 | 標的化したdna配列の核酸塩基を特異的に変換するゲノム配列の改変方法及びそれに用いる分子複合体 |
IL294014B2 (en) | 2015-10-23 | 2024-07-01 | Harvard College | Nucleobase editors and their uses |
DK3382019T3 (da) | 2015-11-27 | 2022-05-30 | Univ Kobe Nat Univ Corp | Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri |
-
2016
- 2016-09-09 JP JP2017538558A patent/JP6664693B2/ja active Active
- 2016-09-09 CN CN201680065297.7A patent/CN108271384B/zh not_active Expired - Fee Related
- 2016-09-09 US US15/757,243 patent/US10767173B2/en active Active
- 2016-09-09 EP EP16844517.9A patent/EP3348638B1/en active Active
- 2016-09-09 BR BR112018004636-1A patent/BR112018004636A2/pt active Search and Examination
- 2016-09-09 ES ES16844517T patent/ES2938623T3/es active Active
- 2016-09-09 WO PCT/JP2016/076711 patent/WO2017043656A1/ja active Application Filing
- 2016-09-09 DK DK16844517.9T patent/DK3348638T3/da active
-
2018
- 2018-10-08 HK HK18112746.2A patent/HK1253544A1/zh unknown
Also Published As
Publication number | Publication date |
---|---|
US10767173B2 (en) | 2020-09-08 |
BR112018004636A2 (pt) | 2018-10-30 |
US20190203198A1 (en) | 2019-07-04 |
WO2017043656A1 (ja) | 2017-03-16 |
JPWO2017043656A1 (ja) | 2018-06-21 |
DK3348638T3 (da) | 2023-02-13 |
EP3348638B1 (en) | 2022-12-14 |
JP6664693B2 (ja) | 2020-03-13 |
ES2938623T3 (es) | 2023-04-13 |
EP3348638A4 (en) | 2019-03-13 |
EP3348638A1 (en) | 2018-07-18 |
CN108271384A (zh) | 2018-07-10 |
CN108271384B (zh) | 2022-04-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
HK1253544A1 (zh) | 通過特異性地轉化靶dna序列的核碱基轉化革蘭氏陽性細菌的基因組序列的方法,以及用於該方法的分子複合物 | |
HK1256888A1 (zh) | 用於特異性轉換靶向dna序列的核酸碱基的單子葉植物的基因組序列的轉換方法、及其中使用的分子複合體 | |
HK1253540A1 (zh) | 用於特異性轉變靶向dna序列的核酸碱基的基因組序列的修飾方法、及其使用的分子複合體 | |
SG11201609211VA (en) | Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same | |
PT3216867T (pt) | Método para modificar a sequência de genoma para introduzir mutação específica a sequência de adn alvo por reação de remoção de bases, e complexo molecular nele utilizado | |
IL273147B (en) | Polynucleotides DNA/RNA hybrids crispr and methods of use | |
IL259788A (en) | Adapted nucleic acid sequences, preparations containing them and methods for determining duplex sequence using them | |
IL254120A0 (en) | Methods for targeted coverage of sequences | |
EP3359691A4 (en) | DEVICES, SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING | |
EP3470529A4 (en) | METHOD FOR ISOTHERMAL NUCLEIC ACID SELF-REINFORCEMENT | |
EP3450439B8 (en) | Method for producing monomer for single-stranded nucleic acid molecule | |
IL246184A0 (en) | Single chain il-12 nucleic acids, polypeptides and their uses | |
GB2537077A (en) | Methods for sequencing nucleic acids | |
ZA201801947B (en) | Compounds as dna probes, methods and applications thereof | |
GB201510723D0 (en) | High throughput isothermal nucleic acid amplification | |
DK3359671T3 (da) | Dna-vektorer, transposoner og transposaser til eukaryot genommodifikation | |
EP3696269A4 (en) | SINGLE STRAND NUCLEIC ACID MOLECULE AND ITS PRODUCTION PROCESS | |
EP3283630A4 (en) | Oligonucleotide fragment, and method as well as application of selective amplifiction of variant of target nucleic acid sequence using the same | |
SG11201705820UA (en) | Gene expression system using stealthy rna, and gene introduction/expression vector including said rna | |
EP3447139A4 (en) | METHOD FOR INCREASING THE MUTATION INTRODUCTION EFFICIENCY IN A GENOMSEQUENCE MODIFICATION METHOD AND MOLECULAR COMPLEX FOR USE THEREOF | |
ZA201707935B (en) | Herbicide-tolerant maize plant dbn9858, and nucleotide sequence and method for detecting same | |
EP3103884A4 (en) | Nucleic acid detection and assay method using mask oligonucleotide, and device for same | |
EP3467835C0 (en) | IMPROVED ALIGNMENT METHOD FOR NUCLEIC ACID SEQUENCES | |
EP3387431A4 (en) | Systems, methods and compositions for improving the specificity of nucleic acid hybridization | |
HK1210906A2 (zh) | 全自動核酸分子雜交儀 |