DK3447139T3 - Fremgangsmåde til øgning af mutationsindføringseffektivitet i genomsekvensmodificeringsteknik, og molekylært kompleks til anvendelse dertil - Google Patents
Fremgangsmåde til øgning af mutationsindføringseffektivitet i genomsekvensmodificeringsteknik, og molekylært kompleks til anvendelse dertil Download PDFInfo
- Publication number
- DK3447139T3 DK3447139T3 DK17786061.6T DK17786061T DK3447139T3 DK 3447139 T3 DK3447139 T3 DK 3447139T3 DK 17786061 T DK17786061 T DK 17786061T DK 3447139 T3 DK3447139 T3 DK 3447139T3
- Authority
- DK
- Denmark
- Prior art keywords
- genomic sequence
- molecular complex
- modification techniques
- sequence modification
- insertion efficiency
- Prior art date
Links
- 238000000034 method Methods 0.000 title 2
- 230000037431 insertion Effects 0.000 title 1
- 238000003780 insertion Methods 0.000 title 1
- 238000012986 modification Methods 0.000 title 1
- 230000004048 modification Effects 0.000 title 1
- 230000035772 mutation Effects 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04005—Cytidine deaminase (3.5.4.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2016085631 | 2016-04-21 | ||
| PCT/JP2017/016105 WO2017183724A1 (ja) | 2016-04-21 | 2017-04-21 | ゲノム配列改変技術における変異導入効率の向上方法、及びそれに用いる分子複合体 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3447139T3 true DK3447139T3 (da) | 2022-07-04 |
Family
ID=60116870
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17786061.6T DK3447139T3 (da) | 2016-04-21 | 2017-04-21 | Fremgangsmåde til øgning af mutationsindføringseffektivitet i genomsekvensmodificeringsteknik, og molekylært kompleks til anvendelse dertil |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20200377910A1 (da) |
| EP (1) | EP3447139B1 (da) |
| JP (1) | JP7001272B2 (da) |
| KR (1) | KR102116200B1 (da) |
| CN (2) | CN109312329B (da) |
| BR (1) | BR112018071376A2 (da) |
| CA (1) | CA3021281C (da) |
| DK (1) | DK3447139T3 (da) |
| ES (1) | ES2919961T3 (da) |
| SG (1) | SG11201809242VA (da) |
| WO (1) | WO2017183724A1 (da) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK3382019T3 (da) | 2015-11-27 | 2022-05-30 | Univ Kobe Nat Univ Corp | Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri |
| KR20200103769A (ko) * | 2018-01-23 | 2020-09-02 | 기초과학연구원 | 연장된 단일 가이드 rna 및 그 용도 |
| CN110607320B (zh) * | 2018-11-23 | 2023-05-12 | 电子科技大学 | 一种植物基因组定向碱基编辑骨架载体及其应用 |
| WO2020241869A1 (ja) * | 2019-05-30 | 2020-12-03 | 国立大学法人東京大学 | ２種の核酸塩基変換酵素が融合されたＣａｓタンパク質を利用したゲノム編集システム |
| US20220333133A1 (en) | 2019-09-03 | 2022-10-20 | Voyager Therapeutics, Inc. | Vectorized editing of nucleic acids to correct overt mutations |
| IL292117A (en) * | 2019-10-17 | 2022-06-01 | Pairwise Plants Services Inc | Variants of cas12a nuclei and methods of production and use thereof |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003251286B2 (en) | 2002-01-23 | 2007-08-16 | The University Of Utah Research Foundation | Targeted chromosomal mutagenesis using zinc finger nucleases |
| JP2013128413A (ja) | 2010-03-11 | 2013-07-04 | Kyushu Univ | Ｐｐｒモチーフを利用したｒｎａ結合性蛋白質の改変方法 |
| JP2015527889A (ja) * | 2012-07-25 | 2015-09-24 | ザ ブロード インスティテュート， インコーポレイテッド | 誘導可能なｄｎａ結合タンパク質およびゲノム撹乱ツール、ならびにそれらの適用 |
| CN111471675A (zh) * | 2014-03-05 | 2020-07-31 | 国立大学法人神户大学 | 特异性转变靶向dna序列的核酸碱基的基因组序列的修饰方法、及其使用的分子复合体 |
| JP6153180B2 (ja) * | 2014-11-04 | 2017-06-28 | 国立大学法人神戸大学 | 脱塩基反応により標的化したｄｎａ配列に特異的に変異を導入する、ゲノム配列の改変方法、並びにそれに用いる分子複合体 |
| WO2017009073A1 (de) * | 2015-07-13 | 2017-01-19 | Huf Hülsbeck & Fürst Gmbh & Co. Kg | Türaussengriff für ein fahrzeug |
| JP6780860B2 (ja) * | 2015-09-09 | 2020-11-04 | 国立大学法人神戸大学 | 標的化したｄｎａ配列の核酸塩基を特異的に変換するゲノム配列の改変方法及びそれに用いる分子複合体 |
| JP6664693B2 (ja) * | 2015-09-09 | 2020-03-13 | 国立大学法人神戸大学 | 標的化したｄｎａ配列の核酸塩基を特異的に変換する、グラム陽性菌のゲノム配列の変換方法、及びそれに用いる分子複合体 |
| IL294014B2 (en) * | 2015-10-23 | 2024-07-01 | Harvard College | Nucleobase editors and their uses |
| DK3382019T3 (da) * | 2015-11-27 | 2022-05-30 | Univ Kobe Nat Univ Corp | Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri |
-
2017
- 2017-04-21 DK DK17786061.6T patent/DK3447139T3/da active
- 2017-04-21 WO PCT/JP2017/016105 patent/WO2017183724A1/ja active Application Filing
- 2017-04-21 JP JP2018513234A patent/JP7001272B2/ja active Active
- 2017-04-21 EP EP17786061.6A patent/EP3447139B1/en active Active
- 2017-04-21 CN CN201780038364.0A patent/CN109312329B/zh active Active
- 2017-04-21 US US16/094,587 patent/US20200377910A1/en active Pending
- 2017-04-21 ES ES17786061T patent/ES2919961T3/es active Active
- 2017-04-21 BR BR112018071376-7A patent/BR112018071376A2/pt active Search and Examination
- 2017-04-21 CA CA3021281A patent/CA3021281C/en active Active
- 2017-04-21 KR KR1020187032638A patent/KR102116200B1/ko active IP Right Grant
- 2017-04-21 CN CN202311476665.5A patent/CN117925730A/zh active Pending
- 2017-04-21 SG SG11201809242VA patent/SG11201809242VA/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20200377910A1 (en) | 2020-12-03 |
| EP3447139B1 (en) | 2022-06-08 |
| CN109312329A (zh) | 2019-02-05 |
| SG11201809242VA (en) | 2018-11-29 |
| CN109312329B (zh) | 2023-11-21 |
| ES2919961T3 (es) | 2022-07-29 |
| CA3021281C (en) | 2021-07-27 |
| BR112018071376A2 (pt) | 2019-04-24 |
| JPWO2017183724A1 (ja) | 2019-02-28 |
| EP3447139A1 (en) | 2019-02-27 |
| CN117925730A (zh) | 2024-04-26 |
| EP3447139A4 (en) | 2019-10-02 |
| JP7001272B2 (ja) | 2022-01-19 |
| KR20180132862A (ko) | 2018-12-12 |
| CA3021281A1 (en) | 2017-10-26 |
| WO2017183724A1 (ja) | 2017-10-26 |
| KR102116200B1 (ko) | 2020-05-27 |
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