DK3234117T3 - Sammensætninger og fremgangsmåder til effektiv genredigering i e.coli ved hjælp af guide-rna/cas-endonukleasesystemer i kombination med cirkelformede polynukleotidmodifikations-templates - Google Patents

Sammensætninger og fremgangsmåder til effektiv genredigering i e.coli ved hjælp af guide-rna/cas-endonukleasesystemer i kombination med cirkelformede polynukleotidmodifikations-templates Download PDF

Info

Publication number
DK3234117T3
DK3234117T3 DK15816605.8T DK15816605T DK3234117T3 DK 3234117 T3 DK3234117 T3 DK 3234117T3 DK 15816605 T DK15816605 T DK 15816605T DK 3234117 T3 DK3234117 T3 DK 3234117T3
Authority
DK
Denmark
Prior art keywords
templication
editring
coli
compositions
efficient
Prior art date
Application number
DK15816605.8T
Other languages
English (en)
Inventor
Ryan L Frisch
Ethel Noland Jackson
Original Assignee
Dupont Us Holding Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dupont Us Holding Llc filed Critical Dupont Us Holding Llc
Application granted granted Critical
Publication of DK3234117T3 publication Critical patent/DK3234117T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DK15816605.8T 2014-12-17 2015-12-02 Sammensætninger og fremgangsmåder til effektiv genredigering i e.coli ved hjælp af guide-rna/cas-endonukleasesystemer i kombination med cirkelformede polynukleotidmodifikations-templates DK3234117T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462092914P 2014-12-17 2014-12-17
PCT/US2015/063434 WO2016099887A1 (en) 2014-12-17 2015-12-02 Compositions and methods for efficient gene editing in e. coli using guide rna/cas endonuclease systems in combination with circular polynucleotide modification templates

Publications (1)

Publication Number Publication Date
DK3234117T3 true DK3234117T3 (da) 2021-06-07

Family

ID=55024260

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15816605.8T DK3234117T3 (da) 2014-12-17 2015-12-02 Sammensætninger og fremgangsmåder til effektiv genredigering i e.coli ved hjælp af guide-rna/cas-endonukleasesystemer i kombination med cirkelformede polynukleotidmodifikations-templates

Country Status (12)

Country Link
US (1) US20170369866A1 (da)
EP (1) EP3234117B1 (da)
JP (1) JP6839082B2 (da)
KR (1) KR102424626B1 (da)
CN (1) CN107250363B (da)
AU (1) AU2015363113B2 (da)
BR (1) BR112017012765A2 (da)
CA (1) CA2971391C (da)
DK (1) DK3234117T3 (da)
ES (1) ES2865268T3 (da)
MX (1) MX2017007907A (da)
WO (1) WO2016099887A1 (da)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2012333134B2 (en) 2011-07-22 2017-05-25 John Paul Guilinger Evaluation and improvement of nuclease cleavage specificity
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9068179B1 (en) 2013-12-12 2015-06-30 President And Fellows Of Harvard College Methods for correcting presenilin point mutations
AU2015217208B2 (en) 2014-02-11 2018-08-30 The Regents Of The University Of Colorado, A Body Corporate CRISPR enabled multiplexed genome engineering
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
WO2017070632A2 (en) 2015-10-23 2017-04-27 President And Fellows Of Harvard College Nucleobase editors and uses thereof
SG11201807025SA (en) * 2016-02-26 2018-09-27 Lanzatech New Zealand Ltd Crispr/cas systems for c-1 fixing bacteria
AU2017280353B2 (en) 2016-06-24 2021-11-11 Inscripta, Inc. Methods for generating barcoded combinatorial libraries
CA3032699A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harvard College Adenosine nucleobase editors and uses thereof
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
CN110114461A (zh) 2016-08-17 2019-08-09 博德研究所 新型crispr酶和系统
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
EP3526320A1 (en) 2016-10-14 2019-08-21 President and Fellows of Harvard College Aav delivery of nucleobase editors
JP7182545B2 (ja) * 2016-12-14 2022-12-02 ヴァーヘニンゲン ユニヴェルシテット 熱安定性ｃａｓ９ヌクレアーゼ
CN110312803B (zh) * 2016-12-21 2024-04-30 许景焜 编辑核酸序列的组合物及方法
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
KR102687373B1 (ko) 2017-03-23 2024-07-23 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
WO2018204777A2 (en) 2017-05-05 2018-11-08 The Broad Institute, Inc. Methods for identification and modification of lncrna associated with target genotypes and phenotypes
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
WO2018226853A1 (en) * 2017-06-07 2018-12-13 The Rockefeller University Enhancement of crispr gene editing or target destruction by co-expression of heterologous dna repair protein
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
CN109295054B (zh) * 2017-07-25 2024-02-06 广州普世利华科技有限公司 用于靶向病原体基因RNA的gRNA及基于C2c2的病原体基因的检测方法及试剂盒
EP3658573A1 (en) 2017-07-28 2020-06-03 President and Fellows of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace)
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
CN111757937A (zh) 2017-10-16 2020-10-09 布罗德研究所股份有限公司 腺苷碱基编辑器的用途
CN109971778B (zh) * 2017-12-27 2022-11-18 北京蓝晶微生物科技有限公司 一种在盐单胞菌中快速基因编辑的载体组合及其应用
AU2019236210A1 (en) 2018-03-14 2020-09-10 Arbor Biotechnologies, Inc. Novel CRISPR DNA targeting enzymes and systems
CA3109083A1 (en) * 2018-08-09 2020-02-13 G+Flas Life Sciences Compositions and methods for genome engineering with cas12a proteins
IL292273B2 (en) 2018-08-14 2023-10-01 Inscripta Inc Devices, modules and methods for improved detection of edited sequences in living cells
US11965154B2 (en) * 2018-08-30 2024-04-23 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
WO2020191249A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
CA3136114A1 (en) 2019-04-05 2020-10-08 Danisco Us Inc. Methods for integrating a donor dna sequence into the genome of bacillus using linear recombinant dna constructs and compositions thereof
CA3136113A1 (en) 2019-04-05 2020-10-08 Danisco Us Inc. Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof
WO2020236967A1 (en) 2019-05-20 2020-11-26 The Broad Institute, Inc. Random crispr-cas deletion mutant
US20220298501A1 (en) 2019-08-30 2022-09-22 The Broad Institute, Inc. Crispr-associated mu transposase systems
MX2022014008A (es) 2020-05-08 2023-02-09 Broad Inst Inc Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo.
CN118541475A (zh) * 2021-12-27 2024-08-23 亘喜生物科技（上海）有限公司 用于细胞修饰的系统和方法
WO2024118881A1 (en) 2022-12-01 2024-06-06 Genencor International Bv Iterative muliplex genome engineering in microbial cells using a bidirectional selection marker system
WO2024118882A1 (en) 2022-12-01 2024-06-06 Genencor International Bv Iterative multiplex genome engineering in microbial cells using a selection marker swapping system
WO2024118876A1 (en) 2022-12-01 2024-06-06 Genencor International Bv Iterative multiplex genome engineering in microbial cells using a recombinant self-excisable selection marker system
WO2024145743A1 (zh) * 2023-01-03 2024-07-11 哈尔滨工业大学 一类rna引导的内切酶系统及其基因编辑应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2419322C (en) * 2000-08-14 2012-10-23 Donald L. Court Enhanced homologous recombination mediated by lambda recombination proteins
WO2005056786A2 (en) * 2003-12-05 2005-06-23 Wisconsin Alumni Research Foundation Sugar kinases with expanded substrate specificity and their use
US20140113376A1 (en) * 2011-06-01 2014-04-24 Rotem Sorek Compositions and methods for downregulating prokaryotic genes
DK2800811T3 (da) * 2012-05-25 2017-07-17 Univ Vienna Fremgangsmåder og sammensætninger til rna-rettet target-dna-modificering og til rna-rettet modulering af transkription
EP3825401A1 (en) * 2012-12-12 2021-05-26 The Broad Institute, Inc. Crispr-cas component systems, methods and compositions for sequence manipulation
US20150132263A1 (en) * 2013-11-11 2015-05-14 Radiant Genomics, Inc. Compositions and methods for targeted gene disruption in prokaryotes

Also Published As

Publication number Publication date
CA2971391C (en) 2023-05-09
AU2015363113B2 (en) 2021-03-11
JP2017538422A (ja) 2017-12-28
US20170369866A1 (en) 2017-12-28
AU2015363113A1 (en) 2017-06-29
BR112017012765A2 (pt) 2018-01-16
MX2017007907A (es) 2017-09-18
CN107250363A (zh) 2017-10-13
ES2865268T3 (es) 2021-10-15
EP3234117A1 (en) 2017-10-25
EP3234117B1 (en) 2021-03-03
KR20170087959A (ko) 2017-07-31
CN107250363B (zh) 2021-03-30
KR102424626B1 (ko) 2022-07-25
JP6839082B2 (ja) 2021-03-03
WO2016099887A1 (en) 2016-06-23
CA2971391A1 (en) 2016-06-23

Similar Documents

Publication Publication Date Title
DK3234117T3 (da) Sammensætninger og fremgangsmåder til effektiv genredigering i e.coli ved hjælp af guide-rna/cas-endonukleasesystemer i kombination med cirkelformede polynukleotidmodifikations-templates
HK1243464B (zh) 使用crispr-cas系統的多核苷酸擴增
DK3134130T3 (da) Sammensætninger og fremgangsmåder til behandling af hæmoglobinopatier
DK3108009T3 (da) Fremgangsmåder og sammensætninger til DNA-profilering
DK3245225T3 (da) Sammensætninger og fremgangsmåder til behandling og detektering af cancere
BR112016018521A2 (pt) composição, e, kit.
DK3206497T3 (da) Sammensætninger og fremgangsmåder til behandling af meibomsk kirtel-dysfunktion
DK3102673T3 (da) Fremgangsmåder og sammensætninger til behandling af beta-talassæmi
ZA201704293B (en) Selective bace1 inhibitors
DK3140967T3 (da) Systemer og fremgangsmåder til forbedringer af træningsfeltdesign for forøgede symbolvarigheder
DK3146035T3 (da) Biofilmmedier, behandlingssystem og behandlingsfremgangsmåde
DK3200815T3 (da) Fremgangsmåder og sammensætninger til behandling af cancer
PL3977996T3 (pl) Leczenie zespołu tourette’a
DK3204386T3 (da) Substituerede aminopurinforbindelser, sammensætninger deraf og fremgangsmåder til behandling dermed
DK3456330T3 (da) Cortexolon-17alpha-valerat til anvendelse ved behandling af tumorer
PL3276029T3 (pl) Stal nierdzewna o doskonałej podatności na lutowanie twarde
DK3110446T3 (da) Fremgangsmåder og sammensætninger til behandling af Siglec-8-associerede sygdomme
BR112017015618A2 (pt) inibidores de mir-92 e usos destes.
DK3122349T3 (da) Sammensætninger til behandling af autodigestion
IL249708B (en) Discs for ventilation and a method for using them
DK3273951T3 (da) Sammensætninger og fremgangsmåder til behandling af psoriasis
ZA201703771B (en) Compositions and methods for treating hyperkalemia
DK3340993T3 (da) Forbindelse til anvendelse i fremgangsmåder til behandling af polycystisk nyresygdom
BR112017024755A2 (pt) sistemas e métodos de dessalinização de humidificação-desumidificação.
HK1251252A1 (zh) 多核苷酸組裝的組合物和方法